CN213223538U - Simple device for washing latex coupled antibody - Google Patents
Simple device for washing latex coupled antibody Download PDFInfo
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- CN213223538U CN213223538U CN202022033207.2U CN202022033207U CN213223538U CN 213223538 U CN213223538 U CN 213223538U CN 202022033207 U CN202022033207 U CN 202022033207U CN 213223538 U CN213223538 U CN 213223538U
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Abstract
The utility model discloses a simple and easy device of latex coupling antibody washing, comprising a base plate, the first spliced pole of the equal fixedly connected with in upper surface both sides of bottom plate, both sides the upper end fixedly connected with mounting panel of first spliced pole, just the equal fixedly connected with second spliced pole in upper surface both sides of mounting panel, both sides the upper end fixedly connected with installing frame of second spliced pole, the upper end fixedly connected with washing liquid reserve tank of installing frame, just the outside fixedly connected with sample liquid reserve tank of washing liquid reserve tank. The device has the advantages of simple structure, convenient operation, high washing efficiency and the like, can wash the solution after the polyclonal antibody is coupled with the polystyrene latex by using the device, can save the links of high-speed centrifugation, ultrafiltration treatment, desalination and the like after coupling, greatly shortens the treatment time, can treat a large amount of liquid, and improves the washing efficiency.
Description
Technical Field
The utility model relates to a latex coupling antibody washing technical field specifically is a simple and easy device of latex coupling antibody washing.
Background
The traditional microsphere latex washing is to obtain a precipitate through centrifugation, and then the obtained precipitate is resuspended by using a buffer solution, the process needs to be repeated for many times, the speed is low, an expensive high-speed centrifuge device is needed, and the uniform monodisperse system is difficult to form through repeated washing.
The device which is compatible with a large amount of liquid change, simple to operate and high in washing efficiency is urgently needed to be developed in the field.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a simple and easy device of latex coupling antibody washing to solve the problem that proposes among the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme:
the simple device for washing the latex coupling antibody comprises a bottom plate, wherein two sides of the upper surface of the bottom plate are fixedly connected with first connecting columns, two sides of the upper end of each first connecting column are fixedly connected with a mounting plate, two sides of the upper surface of the mounting plate are fixedly connected with second connecting columns, two sides of the upper end of each second connecting column are fixedly connected with a mounting frame, the upper end of each mounting frame is fixedly connected with a washing liquid storage tank, and the outer side of each washing liquid storage tank is fixedly connected with a sample liquid storage tank;
the installation cavity has been seted up to the inside of installation frame, install the peristaltic pump in the installation cavity, just the one end of peristaltic pump is connected with first connecting pipe and second connecting pipe respectively, the upper surface fixed connection of the upper end of first connecting pipe and second connecting pipe and installation cavity, both sides be provided with the separation post between the second connecting pipe, the upper end and first connecting pipe and the second connecting pipe lower extreme fixed connection of separation post, both sides be provided with the fraction collector between the first connecting post, be connected through the third connecting pipe between fraction collector and the separation post.
As a further aspect of the present invention: connecting channels are respectively arranged between the upper end of the mounting frame and the washing liquid storage tank and between the upper end of the mounting frame and the sample liquid storage tank, and the first connecting pipe and the second connecting pipe are respectively communicated with the washing liquid storage tank and the sample liquid storage tank through two sides.
As a further aspect of the present invention: the upper end of washing liquid reserve tank is provided with the second filling opening, just the upper end of sample liquid reserve tank is the circumference array and has seted up first filling opening.
As a further aspect of the present invention: and the separation column is internally filled with sephadex G-25.
As a further aspect of the present invention: the model of the peristaltic pump is RL 6000317.
As a further aspect of the present invention: the type of the fraction collector is DBS-100.
Compared with the prior art, the beneficial effects of the utility model are that:
the device has the advantages of simple structure, convenient operation, high washing efficiency and the like, can wash the solution after the polyclonal antibody is coupled with the polystyrene latex by using the device, can save the links of high-speed centrifugation, ultrafiltration treatment, desalination and the like after coupling, greatly shortens the treatment time, can treat a large amount of liquid, and improves the washing efficiency.
Drawings
FIG. 1 is a schematic front view of a simple device for washing latex coupled with antibody.
FIG. 2 is a schematic cross-sectional view of a simple apparatus for washing latex coupled with antibody.
FIG. 3 is a schematic top view of a simple apparatus for washing latex-coupled antibody.
In the figure: the device comprises a bottom plate 1, a first connecting column 2, a mounting plate 3, a fraction collector 4, a second connecting column 5, a separating column 6, a peristaltic pump 7, a mounting frame 8, a first connecting pipe 9, a second connecting pipe 10, a sample liquid storage tank 11, a washing liquid storage tank 12, a connecting channel 13, a mounting cavity 14, a first liquid adding port 15, a second liquid adding port 16 and a third connecting pipe 17.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1 to 3, in the embodiment of the present invention, a simple device for latex-coupled antibody washing comprises a bottom plate 1, wherein both sides of the upper surface of the bottom plate 1 are fixedly connected with first connecting columns 2, the upper ends of the first connecting columns 2 are fixedly connected with a mounting plate 3, both sides of the upper surface of the mounting plate 3 are fixedly connected with second connecting columns 5, the upper ends of the second connecting columns 5 are fixedly connected with a mounting frame 8, the upper end of the mounting frame 8 is fixedly connected with a washing liquid storage tank 12, the outer side of the washing liquid storage tank 12 is fixedly connected with a sample storage tank 11, a mounting cavity 14 is formed inside the mounting frame 8, a peristaltic pump 7 is installed in the mounting cavity 14, one end of the peristaltic pump 7 is respectively connected with a first connecting pipe 9 and a second connecting pipe 10, the upper ends of the first connecting pipe 9 and the second connecting pipe 10 are fixedly connected with the upper surface of the mounting cavity 14, a separating column 6 is arranged, the upper end and the first connecting pipe 9 of separation column 6 and the lower extreme fixed connection of second connecting pipe 10 are provided with fraction collector 4 between the first connecting column 2 of both sides, are connected through third connecting pipe 17 between fraction collector 4 and the separation column 6, carry the inside washing separation that carries out of separation column 6 through first connecting pipe 9 and second connecting pipe 10 respectively with washing liquid and sample in washing liquid reserve tank 12 and the sample liquid reserve tank 11 through peristaltic pump 7 respectively.
Referring to fig. 2, a connection channel 13 is respectively disposed between the upper end of the mounting frame 8 and the washing liquid storage tank 12 and the sample storage tank 11, and the first connection pipe 9 and the second connection pipe 10 are respectively communicated with the washing liquid storage tank 12 and the sample storage tank 11 through the connection channels 13 at two sides.
Referring to fig. 3, a second liquid adding opening 16 is disposed at the upper end of the washing liquid storage tank 12, and first liquid adding openings 15 are circumferentially arranged at the upper end of the sample storage tank 11, so that liquid is added into the washing liquid storage tank 12 through the second liquid adding opening 16, and a sample is added into the sample storage tank 11 through the first liquid adding opening 15.
Referring to FIG. 1, Sephadex G-25 and Sephadex G-25, which have a water absorption of 2.5mL/G dry gel, are packed in a column 6, and the Sephadex is a beaded gel containing a large amount of hydroxyl groups and easily swells in water and an electrolyte solution. The dextran gels of the G-type have various degrees of cross-linking and therefore their swelling and fractionation ranges are different. The swelling capacity of the sephadex is not substantially affected by the presence of salts and detergents, sephadex G-25, separation range: 1000-5000Mr, particle size [ mu ] m: 50-150, mainly used for industrial desalination and buffer exchange.
Referring to fig. 1, peristaltic pump 7 is model number RL 6000317.
Referring to FIG. 1, the fraction collector 4 is of the type DBS-100.
The utility model discloses a theory of operation is:
when the device is used, the washing liquid and the samples in the washing liquid storage tank 12 and the sample storage tank 11 are respectively conveyed to the interior of the separation column 6 through the first connecting pipe 9 and the second connecting pipe 10 for washing and separation through the peristaltic pump 7, and then enter the fraction collector 4 through the third connecting pipe 17 for automatic collection, so that the links of high-speed centrifugation, ultrafiltration treatment, desalination and the like after coupling can be omitted, the treatment time is greatly shortened, a large amount of liquid can be treated, and the washing efficiency is improved;
liquid is added into the washing liquid storage box 12 through the second liquid adding port 16, and a sample is added into the sample storage box 11 through the first liquid adding port 15.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (6)
1. The utility model provides a simple and easy device of latex coupling antibody washing, includes bottom plate (1), its characterized in that: the washing machine is characterized in that two sides of the upper surface of the bottom plate (1) are fixedly connected with first connecting columns (2), the upper ends of the first connecting columns (2) on two sides are fixedly connected with a mounting plate (3), two sides of the upper surface of the mounting plate (3) are fixedly connected with second connecting columns (5), the upper ends of the second connecting columns (5) on two sides are fixedly connected with a mounting frame (8), the upper end of the mounting frame (8) is fixedly connected with a washing liquid storage box (12), and the outer side of the washing liquid storage box (12) is fixedly connected with a sample liquid storage box (11);
installation cavity (14) have been seted up to the inside of installing frame (8), install peristaltic pump (7) in installation cavity (14), just the one end of peristaltic pump (7) is connected with first connecting pipe (9) and second connecting pipe (10) respectively, the upper end of first connecting pipe (9) and second connecting pipe (10) is connected with the last fixed surface of installation cavity (14), both sides be provided with between second spliced pole (5) split column (6), the upper end and first connecting pipe (9) and second connecting pipe (10) lower extreme fixed connection of split column (6), both sides be provided with fraction collector (4) between first spliced column (2), be connected through third connecting pipe (17) between fraction collector (4) and split column (6).
2. The simple apparatus for latex-conjugated antibody washing according to claim 1, characterized in that: connecting channel (13) have been seted up respectively between the upper end of installing frame (8) and washing liquid storage tank (12) and sample liquid storage tank (11), first connecting pipe (9) and second connecting pipe (10) are respectively through both sides connecting channel (13) and washing liquid storage tank (12) and sample liquid storage tank (11) inside intercommunication.
3. The simple apparatus for latex-conjugated antibody washing according to claim 1, characterized in that: the upper end of the washing liquid storage tank (12) is provided with a second liquid adding port (16), and the upper end of the sample storage tank (11) is provided with a first liquid adding port (15) in a circumferential array.
4. The simple apparatus for latex-conjugated antibody washing according to claim 1, characterized in that: and a Sephadex G-25 (Sephadex G-25) is filled in the separation column (6).
5. The simple apparatus for latex-conjugated antibody washing according to claim 1, characterized in that: the model of the peristaltic pump (7) is RL 6000317.
6. The simple apparatus for latex-conjugated antibody washing according to claim 1, characterized in that: the type of the fraction collector (4) is DBS-100.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022033207.2U CN213223538U (en) | 2020-09-16 | 2020-09-16 | Simple device for washing latex coupled antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022033207.2U CN213223538U (en) | 2020-09-16 | 2020-09-16 | Simple device for washing latex coupled antibody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213223538U true CN213223538U (en) | 2021-05-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022033207.2U Active CN213223538U (en) | 2020-09-16 | 2020-09-16 | Simple device for washing latex coupled antibody |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213223538U (en) |
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2020
- 2020-09-16 CN CN202022033207.2U patent/CN213223538U/en active Active
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