CN213222230U - Micro-fluidic device containing probe array and capillary tube with side channel thereof - Google Patents
Micro-fluidic device containing probe array and capillary tube with side channel thereof Download PDFInfo
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- CN213222230U CN213222230U CN202022037260.XU CN202022037260U CN213222230U CN 213222230 U CN213222230 U CN 213222230U CN 202022037260 U CN202022037260 U CN 202022037260U CN 213222230 U CN213222230 U CN 213222230U
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Abstract
The utility model provides a contain probe array micro-fluidic device and take capillary of side passageway thereof, relate to external diagnostic equipment technical field, including main capillary passageway and a plurality of side capillary passageway, a plurality of side capillary passageways are all connected in the lateral wall of main capillary passageway and the interval sets up and are used for leading-in and derive probe solution, the biological probe layer has been laid to the inside wall that main capillary passageway and side capillary passageway correspond, be equipped with the clearance between the adjacent biological probe layer, through connecting a plurality of side capillary passageways at main capillary passageway lateral wall, the realization detects a plurality of indexes simultaneously, and the operation of punching has been avoided, the influence of breach to the detection has also been avoided, simultaneously because the setting of side capillary passageway, make when sealing side capillary passageway blocking piece apart from the detection position relatively far away, the effectual requirement of having reduced to the blocking piece selection material.
Description
Technical Field
The application relates to the technical field of in-vitro diagnostic equipment, in particular to a micro-fluidic device containing a probe array and a capillary tube with a side channel thereof.
Background
The first step in modern medicine is the quantitative diagnosis of disease using scientific techniques, i.e., in vitro diagnostic techniques. Among them, immunodiagnosis technology and molecular diagnosis technology play an important role. Immunodiagnosis realizes the detection of indexes in a sample to be detected through the specific binding of an antigen and an antibody, and the detection process of the most typical double-antibody sandwich method generally comprises four main steps, namely antibody coating, specific binding of the antigen and the antibody, specific binding of the antigen and a fluorescence-labeled secondary antibody, and detection. Nucleic acid detection is a widely used molecular diagnostic technique, and has the advantages of high speed, high sensitivity, good specificity and the like. Nucleic acid detection generally involves three main steps, namely extraction, amplification and detection of nucleic acids. In the current nucleic acid detection process, the problems of large workload, long time consumption and high cost exist, so that the nucleic acid detection cannot be better applied to clinical detection.
Microfluidic technology refers to science and technology involved in systems for processing or manipulating minute volumes of fluids (nanoliters to attoliters in volume) using network structures of microchannels and microstructures (tens to hundreds of microns in size), featuring miniaturization, integration, and portability. Microfluidics is now considered to have great potential for development in biomedical research and has been widely used in immunodiagnosis and molecular diagnostics. The micro-fluidic chip usually uses materials such as silicon chip, glass and high polymer as a substrate, and various micro-channels and microstructures are processed on the substrate material by micro-processing technology. The technical difficulty is high, the processing cost is high, and serious obstacles are brought to the development and application of microfluidic chip products. Compared with the traditional microfluidic chip, the capillary tube as the microchannel carrier has the advantages of small inner diameter, easy processing, low cost and the like, and is widely applied to the fields of chemical analysis, in-vitro diagnosis and the like.
Most of the existing capillary-based immunodiagnosis and molecular diagnosis are single-index detection, and when the capillary-based immunodiagnosis and molecular diagnosis are applied to a sample to be detected with a multi-index detection requirement, the detection workload can be increased. It has been reported that a probe array is fabricated in a capillary tube to simultaneously detect a plurality of indexes, but these methods all have certain disadvantages, such as tedious operation, time-consuming, easy cross contamination, difficult batch fabrication, etc. For example, chinese utility model patent application CN201921324359.9, a microfluidic chip and a capillary tube with holes, which adopts a mode of punching on the side wall of the capillary tube to lay a biological probe layer on the inner wall of the capillary tube, thereby solving the problem of simultaneous testing of multiple indexes, but the punching operation on the capillary tube is very complicated, and the notch caused by the hole opening can generate a certain influence on the detection result.
SUMMERY OF THE UTILITY MODEL
The first aim at of this application provides a capillary of area side passageway that contains probe array micro-fluidic device, it is through connecting a plurality of side capillary channels at main capillary channel lateral wall, realize detecting a plurality of indexes simultaneously, and avoided the operation of punching, the influence of breach to detecting has also been avoided, simultaneously because the setting of side capillary channel, blocking piece distance detection position is relatively far away when making closed side capillary channel, the effectual requirement of having reduced to the blocking piece selection material.
A second objective of the present application is to provide a micro-fluidic device with a probe array, which uses the above capillary with a side channel of the micro-fluidic device with a probe array to detect multiple indexes simultaneously.
The embodiment of the utility model discloses a realize through following technical scheme:
the utility model provides a capillary that contains probe array micro-fluidic device's area side passageway, includes main capillary passageway and a plurality of side capillary passageway, a plurality of side capillary passageways all connect in the lateral wall and the interval setting of main capillary passageway are used for leading-in and derive probe solution, and the biological probe layer has been laid with the inside wall that side capillary passageway corresponds to main capillary passageway, and is adjacent be equipped with the clearance between the biological probe layer.
Furthermore, one end, far away from the main capillary channel, of the side capillary channel is detachably connected with a plugging block.
Further, the main capillary channel and the plurality of side capillary channels are integrally formed.
Furthermore, the main capillary channel and the side capillary channel are made of glass, quartz, silicon, metal, organic polymer materials or biological polymer materials.
Further, the inner diameters of the main capillary channel and the side capillary channel range from 0.001mm to 10 mm.
Further, the biological probe of the biological probe layer is nucleic acid, polypeptide, protein, antigen, polysaccharide, ligand or receptor.
Furthermore, the side capillary channel is located main capillary channel one side and along the radial setting of main capillary.
Furthermore, a biological probe layer is laid on the inner wall of the side capillary channel.
The micro-fluidic device with the probe array comprises a shell and a plurality of capillaries with side channels of the micro-fluidic device with the probe array, wherein the capillaries with the side channels of the micro-fluidic device with the probe array are packaged in the shell, and the capillaries with the side channels of the micro-fluidic device with the probe array are mutually connected in series or in parallel.
The utility model discloses technical scheme has following advantage and beneficial effect at least:
the utility model has reasonable design and simple structure, can fix biological probes with different types, quantities and concentrations according to the needs by connecting a plurality of side capillary channels on the side wall of the main capillary channel, and has simple and convenient fixing process; the carrier used is a capillary tube with low price, the cost is extremely low, and the simultaneous detection of a plurality of indexes is realized; the micro-processing method is not needed for processing the micro-channel and the micro-structure, the chip bonding is not needed, the processing cost and the difficulty are greatly reduced, the punching operation is avoided, the influence of the notch on the detection is also avoided, meanwhile, due to the arrangement of the side capillary channel, the distance between the blocking block and the detection position is relatively far when the side capillary channel is closed, and the requirement on material selection of the blocking block is effectively reduced.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
Fig. 1 is a schematic structural diagram of a capillary tube with a side channel of a microfluidic device containing a probe array according to embodiment 1 of the present invention;
icon: 1-main capillary channel, 2-side capillary channel, 3-biological probe layer and 4-blocking block.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it should be noted that, if the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship which is usually placed when the product of this application is used, the description is only for convenience of description and simplification, but the indication or suggestion that the device or element to be referred must have a specific orientation, be constructed in a specific orientation and be operated is not to be construed as limiting the present invention.
In the description of the present invention, it should be further noted that unless otherwise explicitly stated or limited, the terms "disposed," "mounted," "connected," and "connected" should be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Example 1
As shown in fig. 1, this example provides a side channel-equipped capillary tube of a microfluidic device containing a probe array, which is characterized in that: the biological probe fixing device comprises a main capillary channel 1 and a plurality of side capillary channels 2, wherein the side capillary channels 2 are connected to the side wall of the main capillary channel 1 and arranged at intervals for leading in and leading out probe solution, biological probe layers 3 are laid on the inner side wall of the main capillary channel 1 corresponding to the side capillary channels 2, gaps are formed between every two adjacent biological probe layers 3, the side wall of the main capillary channel 1 is connected with the side capillary channels 2, biological probes of different types, quantities and concentrations can be fixed according to needs, and the fixing process is simple and convenient; the carrier used is a capillary tube with low price, the cost is extremely low, and the simultaneous detection of a plurality of indexes is realized; the micro-processing method is not needed for processing the micro-channel and the micro-structure, the chip bonding is not needed, the processing cost and the difficulty are greatly reduced, the punching operation is avoided, the influence of the notch on the detection is also avoided, meanwhile, due to the arrangement of the side capillary channel 2, the distance between the blocking block 4 and the detection position is relatively far when the side capillary channel 2 is closed, and the requirement on material selection of the blocking block 4 is effectively reduced.
In this embodiment, side capillary passageway 2 is kept away from 1 one end detachable of main capillary passageway and is connected with shutoff piece 4, adopts above design, can improve the accuracy that detects to because shutoff piece 4 is far away from the check point, the effectual material requirement that has reduced 4 to shutoff piece has reduced the cost of whole device.
More optimized, main capillary passageway 1 and a plurality of side capillary passageway 2 integrated into one piece also can adopt the mode combination of concatenation to form certainly in other embodiments, and it does not influence the utility model discloses a realization, the mode that only adopts integrated into one piece can reduce the complexity of making this device.
In the present embodiment, the internal diameters of the main capillary channel 1 and the side capillary channel 2 range from 0.001mm to 10mm, the lengths of the main capillary channel 1 and the side capillary channel 2 are not limited, the lengths of the main capillary channel 1 and the side capillary channel 2 are selected according to actual needs, the length of the main capillary channel 1 is usually between 0.01mm and 10000mm, and the length of the side capillary channel 2 is usually between 0.001mm and 100 mm. The main capillary channel 1 and the side capillary channels 2 are made of glass, quartz, silicon, metal, organic polymer material (such as plastic) or biopolymer material.
The biological probes of the biological probe layer are nucleic acids, polypeptides, proteins (such as antibodies), antigens, polysaccharides, ligands or receptors, and several of the biological probes mentioned above are selected and combined according to the actual needs of the detection.
In this embodiment, the side capillary passage is located main capillary passage one side and along the radial setting of main capillary passage, adopts above design, can reduce the volume that this device occupied effectively.
A preparation and use method of a capillary tube with a side channel of a micro-fluidic device containing a probe array comprises the following specific steps:
it should be noted that the capillary tube used in the method is a glass capillary tube, the inner diameter of the glass capillary tube is 0.4mm, the length of the main capillary tube channel is 10cm, and the glass capillary tube comprises 4 side capillary tube channels.
S0. cleaning the capillary tube, including acid washing and alkali washing, to obtain clean capillary tube;
s1, preparing primary antibodies, antigens and fluorescence-labeled secondary antibodies corresponding to three myocardial markers (cTnI, Myo and CKMB) and preparing a solution for later use;
s2, respectively introducing anti-solutions of cTnI, Myo and CKMB into the side capillary channels to the inner walls of the opposite sides of the main capillary channels in a one-to-one correspondence manner;
s3, placing the capillary tube at a constant temperature of 37 ℃ and carrying out shaking incubation for 0.5 h;
s4, extracting the probe solutions from the side capillary channels respectively;
s5, introducing cleaning fluid from the inlet end of the main capillary channel to clean and dry the whole microchannel;
s6, introducing a casein solution from the inlet end of the main capillary channel and filling the whole capillary;
s7, placing the capillary tube at a constant temperature of 37 ℃ and carrying out shaking incubation for 0.5 h;
s8, discharging the casein solution from the outlet ends of the main capillary channels respectively;
s9, introducing cleaning fluid from the inlet end of the main capillary channel to clean and dry the whole microchannel;
s10, sealing all inlets of the side capillary channels;
s11, mixing antigen solutions of cTnI, Myo and CKMB with a fluorescence labeling secondary antibody solution, and carrying out warm bath for 10min to combine the antigens with corresponding fluorescence labeling secondary antibodies;
s12, introducing a mixed solution of an antigen and a fluorescence-labeled secondary antibody from the inlet end of the main capillary channel and filling the capillary channel with the mixed solution, wherein the antigen and the fluorescence-labeled secondary antibody conjugate in the mixed solution are combined to corresponding primary antibodies;
s13, placing the capillary tube at a constant temperature of 37 ℃ and carrying out shaking incubation for 10 min;
s14, discharging the mixed solution of the antigen and the fluorescence labeling secondary antibody in the capillary from the outlet end of the main capillary channel;
s15, introducing cleaning fluid from the inlet end of the main capillary channel to clean and dry the whole microchannel;
and S16, carrying out fluorescence detection on the biological probe layer at the joint of the capillary side channel and the main channel.
Example 2
The embodiment provides a microfluidic device containing a probe array, which comprises a shell and a plurality of capillaries containing side channels of the microfluidic device containing the probe array provided in embodiment 1, wherein the capillaries containing the side channels of the microfluidic device containing the probe array are packaged in the shell, and the capillaries containing the side channels of the microfluidic device containing the probe array are mutually connected in series or in parallel.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. A side channel-containing capillary tube for a probe array microfluidic device, comprising: including main capillary passageway and a plurality of side capillary passageway, a plurality of side capillary passageways all connect in the lateral wall and the interval setting of main capillary passageway are used for leading-in and derive probe solution, and the biological probe layer has been laid with the inside wall that side capillary passageway corresponds to main capillary passageway, and is adjacent be equipped with the clearance between the biological probe layer.
2. The capillary with side channel of a microfluidic device containing a probe array according to claim 1, wherein: one end of the side capillary channel, which is far away from the main capillary channel, is detachably connected with a plugging block.
3. The capillary with side channel of a microfluidic device containing a probe array according to claim 1, wherein: the main capillary channel and the plurality of side capillary channels are integrally formed.
4. The capillary with side channel of a microfluidic device containing a probe array according to claim 1, wherein: the main capillary channel and the side capillary channels are made of glass, quartz, silicon, metal, organic polymer materials or biological polymer materials.
5. The capillary with side channel of a microfluidic device containing a probe array according to claim 1, wherein: the inner diameters of the main capillary channel and the side capillary channel range from 0.001mm to 10 mm.
6. The capillary with side channel of a microfluidic device containing a probe array according to claim 1, wherein: the biological probe of the biological probe layer is nucleic acid, polypeptide, protein, antigen, polysaccharide, ligand or receptor.
7. The capillary with side channel of a microfluidic device containing a probe array according to claim 1, wherein: the side capillary passage is located main capillary passage one side and along the radial setting of main capillary passage.
8. The capillary with side channel of a microfluidic device containing a probe array according to claim 1, wherein: and a biological probe layer is laid on the inner wall of the side capillary channel.
9. A microfluidic device containing a probe array, characterized in that: the microfluidic device with the probe array comprises a shell and a plurality of the capillaries with the side channels of the microfluidic device with the probe array as claimed in any one of claims 1 to 8, wherein the capillaries with the side channels of the microfluidic device with the probe array are packaged in the shell, and the capillaries with the side channels of the microfluidic device with the probe array are mutually connected in series or in parallel.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202022037260.XU CN213222230U (en) | 2020-09-16 | 2020-09-16 | Micro-fluidic device containing probe array and capillary tube with side channel thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202022037260.XU CN213222230U (en) | 2020-09-16 | 2020-09-16 | Micro-fluidic device containing probe array and capillary tube with side channel thereof |
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| CN213222230U true CN213222230U (en) | 2021-05-18 |
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