CN213209856U - Cell absolute counting sample loading structure, sample loading device and flow cytometer - Google Patents
Cell absolute counting sample loading structure, sample loading device and flow cytometer Download PDFInfo
- Publication number
- CN213209856U CN213209856U CN202021952169.4U CN202021952169U CN213209856U CN 213209856 U CN213209856 U CN 213209856U CN 202021952169 U CN202021952169 U CN 202021952169U CN 213209856 U CN213209856 U CN 213209856U
- Authority
- CN
- China
- Prior art keywords
- flow channel
- absolute
- flow
- parallel plate
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000003990 capacitor Substances 0.000 claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 238000001514 detection method Methods 0.000 claims abstract description 17
- 239000012530 fluid Substances 0.000 claims description 13
- 238000002347 injection Methods 0.000 claims description 7
- 239000007924 injection Substances 0.000 claims description 7
- 239000002699 waste material Substances 0.000 claims description 4
- 238000004891 communication Methods 0.000 claims description 3
- 230000006641 stabilisation Effects 0.000 claims 2
- 238000011105 stabilization Methods 0.000 claims 2
- 239000013558 reference substance Substances 0.000 abstract 1
- 230000000087 stabilizing effect Effects 0.000 description 11
- 238000005259 measurement Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000010453 quartz Substances 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 239000004005 microsphere Substances 0.000 description 2
- 230000002572 peristaltic effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000032683 aging Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000012634 optical imaging Methods 0.000 description 1
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model provides a structure of appearance on cell absolute count, including electric capacity detection unit, runner and sheath liquid runner. Wherein, the sheath liquid flow channel is communicated with the flow channel; part of the flow channel is embedded in a parallel plate capacitor of the capacitance detection unit, and two pole plates of the parallel plate capacitor are close to the outer wall of the flow channel. The sample loading structure measures the number and types of cells in a sample through the capacitance detection unit, determines the volume of the sample through the structure of the flow channel, and realizes high-precision absolute counting of the cells under the condition of no external reference substance, thereby simplifying sample loading operation and reducing the requirement on a sample loading device. And simultaneously, the utility model also provides a device and flow cytometer of appearance are gone up to the absolute count of cell that has this structure of appearance is gone up to the absolute count of cell.
Description
Technical Field
The utility model belongs to the technical field of the cell absolute count technique and specifically relates to a structure of appearance is gone up in cell absolute count to and contain device and the flow cytometer of appearance is gone up in cell absolute count of this structure.
Background
Currently, absolute counts are considered important in clinical applications, such as HIV detection using absolute counts of CD4+ cells to guide disease conditions and drug use. The absolute counting technical scheme of the flow cytometer adopted by the existing clinical end comprises two types: one method is to add absolute counting microspheres, namely microspheres with known concentration are used as reference, and the detection target concentration is obtained through calculation, so that the method has high cost, fussy operation and large error; and secondly, the flow cytometer is used for measuring the volume, and the target concentration is calculated by measuring the volume of the detected sample and the obtained target number. When the second method is used for sample loading, three modes, namely a syringe pump, a high-precision peristaltic pump, a flow sensor and the like, are generally adopted. However, the three sample loading modes have self errors or errors caused by easy deformation, aging and the like.
Disclosure of Invention
To the circumstances, for overcoming prior art's defect, the utility model provides a sample structure on cell absolute count passes through the number and the type of electric capacity detecting element cell in the measurement sample to confirm the sample volume by runner self structure, under the condition of no plus control, realize high accuracy cell absolute count, thereby simplified the operation of advancing kind, and reduced the requirement to sampling device. And simultaneously, the utility model also provides a device and flow cytometer of appearance are gone up to the absolute count of cell that has this structure of appearance is gone up to the absolute count of cell.
The utility model provides a structure, a device and a flow cytometer of appearance are gone up to cell absolute count in appearance structure, a cell absolute count.
Wherein,
the utility model provides a pair of sample structure includes on cell absolute count: the capacitance detection unit comprises a parallel plate capacitor and is connected with an external power supply: the flow channel is partially embedded in an insulating medium layer of the parallel plate capacitor, and the flow guide sample flows in the parallel plate capacitor in a single direction; a sheath fluid flow channel vertically communicated with the flow channel; wherein, the runner and the sheath fluid runner are positioned on the same horizontal plane; the two polar plates of the parallel plate capacitor are respectively parallel to and close to the outer wall of the flow channel.
The utility model provides a structure of appearance on cell absolute count adopts electric capacity detection unit to replace current optical imaging unit to measure the interior cell of sample, based on electric capacity detection technique, confirms the sample volume by runner self structure, under the condition of no plus control thing, realizes the absolute count of high accuracy cell.
Further, the flow channel embedded in the parallel plate capacitor insulating dielectric layer in the cell absolute counting sample loading structure has a determined cross-sectional area.
The utility model discloses owing to adopt electric capacity detection unit to carry out the electric capacity measurement to sample in the runner, through the runner cross sectional area who injects the electric capacity measuring range, can be by this sample loading structure self confirm the sample volume that corresponds.
Further, the flow channel of the cell absolute counting loading structure is in a straight tube shape. The calculation of the cross-sectional area is facilitated.
Further, the flow channel of the cell absolute counting loading structure comprises: the buffer cavity is used for sample injection; one end of the steady flow cavity is communicated with the buffer cavity, and only single cells are allowed to pass through the steady flow cavity.
Further, the sheath fluid flow channel of the cell absolute counting sample loading structure is communicated with the steady flow cavity close to the buffer cavity.
The utility model discloses the sheath liquid that flows in the well sheath liquid runner can disperse the cell in the sample, makes the sample cell flow through in proper order after the separation and measures the region.
Further, the two electrode plates of the parallel plate capacitor of the cell absolute counting sample loading structure are arranged on two sides of the current stabilizing cavity.
Further, the other end of the steady flow cavity of the cell absolute counting and loading structure is provided with a necking structure.
The utility model discloses a throat structure that the stationary flow chamber discharge end of runner set up cushions the outflow of stationary flow intracavity sample liquid, supplementary continuity and the stability of guaranteeing stationary flow intracavity liquid flow.
In addition to this, the present invention is,
the utility model also provides a cell absolute counting sample loading device, which comprises the cell absolute counting sample loading structure and a shell, wherein, the flow channel and the sheath fluid flow channel are fixed in the shell along the horizontal direction of the shell; two polar plates of the parallel plate capacitor are fixed in the shell along the vertical direction of the shell; the two ends of the flow channel are matched and communicated with a sample inlet and a waste liquid outlet which are arranged on the side wall of the shell; the end part of the sheath liquid flow channel is communicated with a sheath liquid inlet arranged on the side wall of the shell.
The sample loading structure is packaged by the shell in absolute cell counting, the sample loading structure can be effectively isolated from the external environment, the influence of the environment on the measurement precision of the capacitance detection unit is avoided, the sample loading structure can be effectively protected, and the service life of the sample loading structure is prolonged.
At the same time, the user can select the desired position,
the utility model also provides a flow cytometer that has aforementioned cell absolute counting and goes up appearance structure, or adopts aforementioned cell absolute counting to go up appearance device.
The utility model discloses an use the structure of appearance on the absolute count of cell in the flow cytometer, utilize its ability that need not add reference object and self confirm the sample volume, simplified the operation of appearance of going up, expanded the chooseing for use scope to sampling device.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
Fig. 1 is a schematic top view of the structure of the present invention in embodiment 1.
Fig. 2 is a schematic perspective view of the present invention in embodiment 1.
Fig. 3 is a schematic view of a connection structure of the flow channel and the sheath fluid flow channel according to the present invention in embodiment 1.
Fig. 4 is a schematic top view of the structure of the present invention in embodiment 2.
Fig. 5 is a schematic perspective view of the present invention in embodiment 2.
Reference numerals: 1. the device comprises a flow channel, 11 buffer cavities, 12 current stabilizing cavities, 2 sheath liquid flow channels, 3 parallel plate capacitors, 31 cathode plates, 32 anode plates, 33 insulating medium layers and 4 shells.
Detailed Description
In the following, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the embodiments of the present invention. Accordingly, the drawings and description are to be regarded as illustrative in nature, and not as restrictive.
In the description of the embodiments of the present invention, it should be understood that the terms "upper", "lower", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only used for convenience in describing the embodiments of the present invention and simplifying the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the embodiments of the present invention.
In the embodiments of the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly, e.g., as fixed or detachable connections or as an integral part; the connection can be mechanical connection, electrical connection or communication; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the embodiments of the present invention can be understood by those skilled in the art according to specific situations.
In embodiments of the invention, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features through another feature not in direct contact. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly above and obliquely above the second feature, or simply meaning that the first feature is at a lesser level than the second feature.
The following disclosure provides many different embodiments or examples for implementing different configurations of embodiments of the invention. In order to simplify the disclosure of embodiments of the present invention, the components and arrangements of specific examples are described below. Of course, they are merely examples and are not intended to limit embodiments of the present invention. Furthermore, embodiments of the present invention may repeat reference numerals and/or reference letters in the various examples for purposes of simplicity and clarity and do not in themselves dictate a relationship between the various embodiments and/or arrangements discussed.
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
Example 1
As shown in fig. 1 and fig. 2, the present invention provides a sample loading structure for absolute cell count, including: the device comprises a capacitance detection unit, a flow channel 1 and a sheath liquid flow channel 2.
Wherein,
the capacitance detection unit comprises a parallel plate capacitor 3, two pole plates of which are respectively connected with an external constant voltage power supply to form a cathode plate 31 and an anode plate 32, and an insulating medium layer 33 is filled between the two pole plates.
As shown in fig. 3, the flow channel 1 is in a straight tube shape and includes a buffer cavity 11 and a steady flow cavity 12 which are communicated with each other. Wherein, the inner diameter of the buffer cavity 11 is larger, and the end part is used for sample injection; the flow stabilizing cavity 12 is small in inner diameter and only allows single cells to pass through. The end of the flow-stabilizing cavity 12 has a necking structure from which the sample flows out.
The sheath liquid flow channel 2 is also in a straight tube shape, is positioned on the same horizontal plane with the flow channel 1, and is vertically communicated with the flow channel 1. The junction of the sheath liquid flow channel 2 and the flow channel 1 is positioned at a steady flow cavity 12 close to the buffer cavity 11.
Part of the current stabilizing cavity is embedded in the insulating medium layer 33, and the cathode plate 31 and the anode plate 32 are respectively close to the outer wall of the current stabilizing cavity.
During manufacturing, firstly, the length of a current stabilizing cavity embedded in the flat capacitor is determined to determine the cross-sectional area of the current stabilizing cavity, and then the whole flow channel, the sheath fluid flow channel and the parallel plate capacitor are designed. According to the determined design index, PDMS material is respectively adopted to manufacture the flow channel and the sheath fluid flow channel, and the gold electrode and the quartz layer are adopted to manufacture the parallel plate capacitor. And (3) adopting laser to open a through hole in the quartz layer, wherein the through hole is superposed with the central axis of the quartz layer as much as possible. And a flow stabilizing cavity part with a determined length of the flow channel is embedded into the through hole and fixed.
When in use, the gold electrode is connected with an external constant voltage power supply and a bridge tester. And respectively connecting a sample and sheath liquid at the end part of the buffer cavity and the end part of the sheath liquid flow channel. The sample flows from the buffer cavity to the flow stabilizing cavity and is mixed with the sheath fluid in the flow stabilizing cavity. The cells in the sample are dispersed and flow as single cells into the parallel plate capacitor.
After the sample cell enters the parallel plate capacitor, a change in the capacitance of the capacitor results. The capacitance value is recorded by the bridge tester. The DNA content varies from cell to cell, resulting in a change in capacitance. According to the following formula:
wherein C represents capacitance, Q represents charge amount, UA-UBRepresenting the pressure difference between the two plates, epsilonrThe dielectric constant is shown, S represents the cross-sectional area of a current stabilizing cavity embedded in the flat capacitor, k represents the constant of the electrostatic force, and d represents the distance between two polar plates.
The Q value calculated by the above formula is the cell number, which is represented by epsilonrThe values can determine the cell type distribution.
Therefore, when the absolute cell counting sample loading structure is used for counting, no contrast is needed to be added, and the sample injection volume can be determined by the absolute cell counting sample loading structure without external measurement, so that the absolute cell counting sample loading structure is used for sample detection, the operation before sample injection can be simplified, and the requirement of a sample injection device can be reduced.
Example 2
As shown in fig. 4 and 5, the present embodiment provides a cell absolute count loading device.
The cell absolute count loading device adopts a shell 4 to encapsulate the cell absolute count loading structure provided in example 1.
The flow channel 1 and the sheath liquid flow channel 2 are horizontally fixed in the shell 4, and two electrodes of the parallel plate capacitor 3 are vertically fixed in the shell 4.
And a sheath liquid inlet, a sample inlet and a waste liquid outlet are formed in the side wall of the shell 4 and are respectively communicated with the buffer cavity end part and the steady flow cavity end part of the sheath liquid flow channel 2 and the flow channel 1 and are used for connecting an external control pipeline to realize one-way stable flow guide of the sheath liquid and the sample.
The housing 4 is made of a coating, a grounding material or a structure, and provides an electric shield for the parallel plate capacitor 3 to prevent the external environment from interfering with the measurement result. As a preferred scheme, a temperature and humidity control structure or device can be added in the shell, so that the accuracy of the measurement structure is further ensured.
Preferably, a socket set connected with the electrode plate can be arranged on the surface of the shell 4 for quick connection of an external power supply or a testing device with the electrode plate.
When the device is used, the shell sheath fluid inlet, the sample inlet and the waste fluid outlet are respectively connected with corresponding pipelines. The front end of the connecting pipeline at the sample inlet can select a contact type sample feeding device such as the prior injection pump, a non-contact type sample feeding device such as a peristaltic pump and other various sample feeding devices suitable for micro-sample feeding.
Example 3
The present embodiments provide a flow cytometer.
The flow cytometer has the cell absolute count loading structure provided in example 1, or the cell absolute count loading device provided in example 2 is used.
When the absolute cell counting and sample loading device provided by the embodiment 2 is adopted, the shell can be made of a light-transmitting material, so that the absolute cell counting and sample loading device can be used together with an existing optical detection system of a flow cytometer, and the detection efficiency and the detection precision are further improved.
Claims (10)
1. An absolute cell count loading structure, comprising:
the capacitance detection unit comprises a parallel plate capacitor and is connected with an external power supply:
the flow channel is partially embedded in an insulating medium layer of the parallel plate capacitor, and a guide sample flows in the parallel plate capacitor in a single direction;
a sheath fluid flow channel vertically communicating with the flow channel;
wherein the flow channel and the sheath fluid flow channel are positioned on the same horizontal plane; and two polar plates of the parallel plate capacitor are respectively parallel to and close to the outer wall of the flow channel.
2. The absolute cell count loading structure of claim 1 wherein the flow channel embedded within the parallel plate capacitor insulating dielectric layer has a defined cross-sectional area.
3. The absolute cell count loading structure according to claim 1, wherein said flow channel is a straight tube.
4. The absolute cell count loading structure of claim 1, wherein the flow channel comprises:
the buffer cavity is used for sample injection;
and one end of the steady flow cavity is communicated with the buffer cavity, and only single cells are allowed to pass through the steady flow cavity.
5. The absolute cell count loading structure of claim 4, wherein the sheath fluid flow channel is in communication with a flow stabilization chamber adjacent to the buffer chamber.
6. The absolute cell count loading structure according to claim 4, wherein the two plates of the parallel plate capacitor are disposed on both sides of the flow stabilization chamber.
7. The absolute cell count loading structure according to claim 4, wherein the other end of the flow-stabilizing chamber has a constriction structure.
8. A cell absolute count loading device, comprising: the absolute cell count loading structure of any one of claims 1 to 7 and a housing,
wherein,
the flow channel and the sheath liquid flow channel are fixed inside the shell along the horizontal direction of the shell;
the two polar plates of the parallel plate capacitor are fixed in the shell along the vertical direction of the shell;
the two ends of the flow channel are in matched communication with a sample inlet and a waste liquid outlet which are arranged on the side wall of the shell;
the end part of the sheath liquid flow channel is communicated with a sheath liquid inlet arranged on the side wall of the shell.
9. A flow cytometer comprising the cell absolute count loading structure according to any one of claims 1 to 7.
10. A flow cytometer comprising the cell absolute count loading apparatus of claim 8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021952169.4U CN213209856U (en) | 2020-09-09 | 2020-09-09 | Cell absolute counting sample loading structure, sample loading device and flow cytometer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021952169.4U CN213209856U (en) | 2020-09-09 | 2020-09-09 | Cell absolute counting sample loading structure, sample loading device and flow cytometer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213209856U true CN213209856U (en) | 2021-05-14 |
Family
ID=75845007
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021952169.4U Active CN213209856U (en) | 2020-09-09 | 2020-09-09 | Cell absolute counting sample loading structure, sample loading device and flow cytometer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213209856U (en) |
-
2020
- 2020-09-09 CN CN202021952169.4U patent/CN213209856U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8230736B2 (en) | Level sensor for conductive liquids | |
| US8535499B2 (en) | Microfabricated liquid-junction reference electrode | |
| CN101248350B (en) | Hydrogen quantity sensor and hydrogen storage device using the same | |
| US10234435B2 (en) | Conductivity detector and ion chromatography system including the same | |
| AU2022218568B2 (en) | Electrochemical sensor with opening between solid elements | |
| US3322662A (en) | Electrolytic oxygen sensor | |
| US8608925B2 (en) | Multiple-electrode ionic probe | |
| CN213209856U (en) | Cell absolute counting sample loading structure, sample loading device and flow cytometer | |
| US20160230285A1 (en) | Plating apparatus and sensing device using same | |
| CN213875514U (en) | Measuring device for membrane ion migration number | |
| EP2932249B1 (en) | An arrangement for an electrochemical measurement | |
| CN201043962Y (en) | Oxygen sensor | |
| US4973395A (en) | Humidified high sensitivity oxygen detector | |
| US5053116A (en) | Device for humidifying gases without changing oxygen content | |
| EP4407721A1 (en) | Sensor for measuring state of charge of a redox flow battery | |
| JP6831839B2 (en) | Measurement of electrical signals to detect the presence or flow of electrically active species in solution | |
| JP6216689B2 (en) | Reference electrode | |
| CN201477068U (en) | Salt electrolysis cell | |
| CN221326408U (en) | Oxygen analyzer | |
| JP6279389B2 (en) | Measuring system | |
| JPH089638Y2 (en) | Flow cell of electrolyte measuring device | |
| CN217133028U (en) | Electrochemical gas sensor | |
| WO2024027668A1 (en) | Sample test card | |
| CN202372426U (en) | Underground fluid density measuring device | |
| TW202406199A (en) | Flow battery state of health indicator |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |