CN213148947U - Colloidal gold detection reagent card - Google Patents

Abstract

The utility model relates to the field of medical equipment, especially, relate to a colloidal gold detection reagent card. The reagent card includes lid and membrane strip, colloidal gold and connecting piece, and membrane strip, colloidal gold and connecting piece are all established in the lid, the lid is equipped with first application of sample hole and second application of sample hole, first application of sample hole is corresponding with the membrane strip, second application of sample hole corresponds with the colloidal gold, the connecting piece is used for connecting membrane strip and colloidal gold pad. This openly through design this kind will first application of sample hole corresponds with the membrane strip, second application of sample hole corresponds with the colloid metallography, and the sample can not be through the structure of colloidal gold, directly reachs the membrane strip, detects with this kind of reagent card, can reduce sample use amount widely.

Description

Colloidal gold detection reagent card

Technical Field

The utility model relates to the field of medical equipment, especially, relate to a colloidal gold detection reagent card.

Background

The immunochromatography technology is a rapid clinical detection technology which is developed rapidly in the medical field at home and abroad in recent years, and has the characteristics of rapid detection, simple operation, low cost and the like. The test paper strip is the main part that detects liquid, and the function of current reagent card shell is mainly for the test paper strip provides the space of placing, keeps the cleanness of test paper strip.

In the chromatographic detection, a reagent card is mainly used for detecting sample liquid. The detection reagent card of prior art includes upper cover, base and test paper, covers on and is equipped with the application of sample hole and reads the window, is equipped with the test paper groove on the base, and test paper locates the test paper inslot, and the upper cover is connected with the base. However, the existing detection reagent card needs a large amount of samples in the using process, is mostly not suitable for detecting samples which are easy to generate background interference, such as whole blood samples, and simultaneously needs a long time and has low detection efficiency.

SUMMERY OF THE UTILITY MODEL

In order to overcome the deficiencies of the prior art, the embodiments of the present invention provide a detection reagent card with less sample size requirement.

In a first aspect, an embodiment of the present disclosure provides a reagent card, the reagent card includes lid and membrane strip, colloidal gold and connecting piece, and membrane strip, colloidal gold and connecting piece are all established in the lid, the lid is equipped with first application of sample hole and second application of sample hole, first application of sample hole is corresponding with the membrane strip, the second application of sample hole corresponds with the colloidal gold, the connecting piece is used for connecting membrane strip and colloidal gold pad.

In one embodiment, the liquid passing through the colloidal gold pad flows onto the membrane strip through the connecting piece.

In one embodiment, the position of the colloidal gold pad is higher than that of the membrane strip.

In one embodiment, the connecting piece is of a membrane strip structure, the colloidal gold pad is placed on one side of the connecting piece, and the membrane strip is arranged on the other side of the connecting piece.

In some embodiments, the connecting member is made of the same material as the membrane strip.

In some embodiments, the connector is integrally formed with the membrane strip.

In some embodiments, a sample pad is further included; the colloidal gold pad is placed at one end of the membrane strip, one end of the sample pad is abutted to the membrane strip, and the other end of the sample pad corresponds to the first sample adding hole.

In some embodiments, the reagent card includes a plurality of membrane strips, the cover has a plurality of observation windows corresponding to the membrane strips, and the membrane strips are connected to each other.

In some embodiments, the first loading aperture corresponds to at least two membrane strips.

In some embodiments, at least two membrane strips are connected to each other, the connection point of the interconnected membrane strips is a first connection center, and the first sample application hole corresponds to the position of the first connection center.

In some embodiments, the joints of all of the interconnected membrane strips are the same.

In some embodiments, all of the membrane strips are connected to each other.

In some embodiments, the test strip structure differs in the type of at least two membrane strips.

In some embodiments, the coating on at least two of the film strips is different.

In some embodiments, the coating is different on all of the membrane strips.

In some embodiments, one membrane strip corresponds to one colloidal gold.

In some embodiments, one colloidal gold corresponds to a plurality of membrane strips.

In some embodiments, the reagent card further comprises a bibulous pad.

In some embodiments, the reagent card further comprises a PVC plate.

In some embodiments, the colloidal gold pad, the membrane strip and the absorbent pad are sequentially laid on the PVC plate, the absorbent pad is placed at one end of the membrane strip, and the colloidal gold pad is placed at the other end of the membrane strip.

In some embodiments, the reagent card is further covered with a waterproof membrane.

In some embodiments, the waterproofing membrane is a PE membrane.

In some embodiments, the PE film is covered over the film strip and the colloidal gold pad.

In some embodiments, one end of the PE film is 2-3mm beyond one end of the colloidal gold pad, and the other end of the PE film is flush with the other end of the colloidal gold pad.

In some embodiments, the reagent card further comprises a blood filter pad disposed within the cover body.

In some embodiments, the blood filter pad is positioned below the first well and abuts the sample pad.

In some embodiments, the cover includes an upper cover and a lower cover.

In some embodiments, the upper cover is provided with a first protrusion portion, the observation window and the first sample adding hole are arranged on the upper cover, the first protrusion portion is located at one end of the observation window, and at two sides of the first sample adding hole, and the first protrusion portion is used for placing the sample pad.

In some embodiments, the sample pad has an area larger than the area of the blood filter pad and is superposed on the blood filter pad.

In some embodiments, the lower cover is provided with a clamping position for placing the membrane strip and the colloidal gold.

In some embodiments, the size of the screens matches the size of the membrane strip and colloidal gold.

In some embodiments, a second protrusion is disposed between the two blocking positions near the first sample adding hole, and is used for separating the colloidal gold and the membrane strip.

In some embodiments, the lower cover is provided with 1 or more grooves corresponding to the second sample application holes.

In some embodiments, more than 2 grooves are provided, including a first groove and a second groove, the first groove is provided right below the second sample adding hole, and the second groove is provided between the first groove and the clamping position.

In yet another aspect, the present disclosure provides a method of detecting a sample, comprising: adding the first solution to the membrane strip;

adding the second solution to the colloidal gold pad;

the second solution brings the colloidal gold in the colloidal gold pad to the membrane strip.

In some embodiments, the addition of the second solution is followed by the addition of the first solution.

In some embodiments, the first solution is added 2-10min before the second solution is added, with a time of 8-20 mm.

In some embodiments, the first solution is different from the second solution.

In some embodiments, the first solution is a test sample.

In some embodiments, the test sample is selected from saliva, whole blood, interphalangeal blood, serum, or urine.

Some embodiments include a reagent card, the reagent card including a cover and a strip structure, the strip structure being disposed within the cover, the strip structure including a membrane strip and a colloidal gold pad.

In some embodiments, the method of detection comprises using the reagent card.

It will be appreciated that any of the methods of the second aspect may be applied to the reagent card of any of the embodiments of the first aspect, or to other structures.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure.

The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical means of the present invention more clearly understood, the present invention may be implemented in accordance with the content of the description, and in order to make the above and other objects, features, and advantages of the present invention more clearly understandable, the following preferred embodiments are described in detail with reference to the accompanying drawings.

Drawings

Fig. 1 is a schematic diagram of the overall structure of a reagent card according to an exemplary embodiment.

Fig. 2 is a schematic diagram of the overall structure of a reagent card according to an exemplary embodiment.

FIG. 3 is a schematic diagram of the structure of the reagent card cover according to an exemplary embodiment.

FIG. 4 is a schematic view of a reagent card bottom cover according to an exemplary embodiment.

Fig. 5 is a schematic structural diagram of a test strip according to an exemplary embodiment.

The names of the components identified in the figures are as follows:

the lower cover 1, a first groove 101, a second groove 107, a clamp 102, a clamp 103, a fixed column 104 and a second limiting part 105; the second boss 106;

the device comprises an upper cover 2, a first observation window 201, a second observation window 202, a first sample adding hole 203, a second sample adding hole 204, a first boss 205, a first limit part 206, a third limit part 207 and a mounting rod 208;

test paper strip 3, quality control line 301; detection line 302, detection line 303, detection line 304, detection line 305, detection line 306; test strip 4, quality control line 401; detection line 402, detection line 403, detection line 404, detection line 405, detection line 406;

the test paper strip, the PVC plate 311, the absorbent pad 312, the membrane strip 313 and the colloidal gold pad 314;

and a label 9.

Detailed Description

To further illustrate the technical means and effects of the present invention adopted to achieve the intended purpose of the present invention, the following detailed description of the embodiments, structures and features according to the present invention will be made with reference to the accompanying drawings and preferred embodiments.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted", "connected" and "placed" are to be interpreted broadly, for example, as being either fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements.

The term "communicate" is also to be understood broadly, i.e., may be direct or indirect via an intermediary. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Furthermore, the technical features mentioned in the different embodiments of the invention described below can be combined with each other as long as they do not conflict with each other.

As used in this disclosure and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any and all possible combinations of one or more of the associated listed items.

The terms "plurality" and "a plurality" in the present disclosure and appended claims refer to two or more than two unless otherwise specified.

In this disclosure, first application of sample hole is corresponding to the membrane strip and includes that first application of sample hole sets up with the membrane strip is relative promptly, perhaps sets up the pipe between first application of sample hole and the membrane strip, pipe one end and first application of sample hole intercommunication, the other end sets up with the membrane strip is relative. Or a sample pad is disposed between the first well and the membrane strip. The corresponding arrangement mode is various, and the disclosure is not limited in any way as long as the liquid flows onto the membrane strip after passing through the first sample adding hole.

In the present disclosure, the developing solution functions to chromatographe the gold colloid in the colloidal gold pad on the membrane strip by contacting the gold colloid pad.

In some embodiments, since the developing solution is different based on different purposes, for example, the reagent card of the present disclosure is used to detect allergens (pollen, mites, peanuts, etc.), the developing solution may be composed of: 0.2301g of disodium hydrogen phosphate, 0.0456g of sodium dihydrogen phosphate and 0.9g of sodium chloride, and ultrapure water is added to the solution until the volume is 100 mL. However, the composition of the developing solution is not limited at all.

In some embodiments, the reagent card includes a cover body (the cover body includes an upper cover and a lower cover), and a test strip structure (the test strip structure includes a colloidal gold pad, a membrane strip and a connecting member), the test strip structure is disposed between the upper cover and the lower cover, the test strip structure includes a plurality of test strips, a plurality of observation windows corresponding to the test strips one to one are disposed on the upper cover, the test strips are connected to each other, a first sample adding hole is disposed on the upper cover, and the first sample adding hole corresponds to a connection portion of the test strips. According to the reagent card, one sample adding hole can correspond to a plurality of test strips, so that the purpose of simultaneously detecting the plurality of test strips can be achieved through one sample adding hole, the sample amount can be saved, and the detection efficiency can be improved. It should be understood that although the test paper is in the form of a strip in the present embodiment, it is not limited to a strip-shaped structure and may be square, circular, or the like.

In some embodiments, the upper cover is provided with a plurality of sample adding holes, wherein a part of the sample adding holes correspond to a part of the test strips; the other part of the sample adding holes corresponds to the other part of the test strips. For example, there are 1 well for 4 of the strips and the other 1 well for the other 5 strips. Therefore, 1 reagent card disclosed by the invention can be used for detecting various types of samples simultaneously or 1 reagent card can be used for detecting multiple same samples simultaneously, so that the detection efficiency is greatly improved. The various types of samples include, for example, pollen allergens, peanut allergens, mite allergens, and the like, simultaneously.

Fig. 1 shows the reagent card for colloidal gold assay according to a preferred embodiment of the present invention, which utilizes the chromatography of the test strip to detect the properties of the tested liquid, and thus the disease condition can be obtained from the test result. The colloidal gold detection reagent card comprises an upper cover 2, a lower cover 1 connected with the upper cover 2 in a matching way, and a test strip 3 and a test strip 4 which are arranged between the upper cover 2 and the lower cover 1. The types of the test strips 3 and 4 can be the same or different. The upper cover 2 is provided with two observation windows, namely a first observation window 201 and a second observation window 202. The first observation window 201 and the second observation window 202 are used for observing the flowing condition and the chromatography result of the tested liquid sample. The test strip 3 is provided with 5 detection lines and a quality control line which are a quality control line 301 in sequence; detection line 302, detection line 303, detection line 304, detection line 305, detection line 306. The test strip 4 is provided with 5 detection lines and a quality control line which are respectively a quality control line 401; detection line 402, detection line 403, detection line 404, detection line 405, and detection line 406. After the upper cover 2 and the lower cover 1 are tightly closed, the test strip 3 and the test strip 4 are clamped inside, and accurate detection results can be obtained.

The colloidal gold detection reagent card also comprises a first sample adding hole 203 for adding a sample to be detected; the first sample application hole 203 is located between the first observation window 201 and the second observation window 202, at a position close to one end of the detection line 306 or the detection line 406, and at a position far from one end of the quality control line 301 or the quality control line 401. The first well 203 is positioned to ensure that the sample introduced therefrom can flow to the strip 3 and the strip 4, respectively. After the sample is added into the first sample adding hole 203, the sample can flow to the test strip 3 and the test strip 4 simultaneously, and gradually passes through the detection line and the quality control line through chromatography, so that the color is correspondingly developed, and the purpose of detection is achieved.

In another embodiment, as shown in fig. 2, the reagent card for gold assay further comprises a second well 204, the second well 204 is aligned with the axis of the first well 203, and the second well 204 is located at the lower end of the first well 203. The liquid added from the second sample hole 204 can flow through the first sample hole 203 to reach the test strip 3 and the test strip 4, and color development is carried out through chromatography, so that the purpose of detection is achieved. The upper cover 2 also comprises a label 9 which can be used for marking sample information and the like. In one embodiment, as shown in fig. 3, the inner surface of the upper cover 2 of the gold colloid detection reagent card is further provided with two protrusions, namely a protrusion 205 and a protrusion 206, which are respectively adjacent to the lower edges of the first observation window 201 and the second observation window 202, and are located at two sides of the first sample adding hole 203, and can be used for placing a sample pad. The protrusions 205 and 206 may be square in shape and separated into 6 compartments by a cross bar, respectively. A first limiting part 206 is arranged on one end of the inner surface of the upper cover 2, which is far away from the second sampling hole 204, along two sides of the length direction of the observation window; the first limiting portion 206 may be composed of a limiting rod, a limiting plate or a limiting block, and the number of the first limiting portion 206 is not limited, and the first limiting portion 206 is used for fixing the position of the test strip. The periphery of the first sample adding hole 203 is further provided with a third limiting portion 207, the third limiting portion 207 may be composed of a limiting rod, a limiting plate or a limiting block, and the number is not limited, for example, the number may be 6. The third stopper 207 serves to fix the position of the blood filter pad. The inner surface of the upper cover 2 is provided with a plurality of mounting rods 208; the mounting rods 208 may be respectively disposed on both sides of the inner surface of the upper cover 2 and between the first observation window 201 and the second observation window 202, and the number is not limited. The mounting rod 210 is used for fixing and assembling with the lower cover 1.

The lower cover 1 corresponding to the upper cover 2 of the colloidal gold test reagent card is shown in fig. 4. The lower cap 1 is provided with a first recess 101 corresponding to the second well 204 in the upper cap 2, and the first recess 101 can be used to store the storage liquid fed from the well 204. The grooves may be plural, for example, further including the second groove 107. The size of the first groove 101 facing the second well 204 is larger than the size of the second groove 107. The small size of the second recess 107 will provide a buffer for the incoming developing liquid, thereby preventing excess liquid from flowing into the entire card and affecting the detection result. The lower cover 1 is further provided with a detent 103 and a detent 102 corresponding to the first observation window 201 and the second observation window 202. The lower cover 1 is further provided with a fixing column 104 matched with the mounting rod 210, the fixing column 104 is a hollow column, and the mounting rod 210 is inserted into the fixing column 104 to buckle the upper cover and the lower cover. The periphery of the block 102 is provided with a second limiting part 105, which can fix the test strip in the block.

In some embodiments, the lower cover 1 has a second protrusion 106 between the two detents 102 and 103 facing the first well for separating the two strip test strip structures; and has a certain supporting function for the sample pad.

In one embodiment, the structure of the test strip 3 or 4 is as shown in fig. 5, the test strip 3 or 4 includes a water absorption pad 312, a membrane strip 313, a colloidal gold pad 314 and a PVC plate 311, the water absorption pad 312 is attached above the PVC plate 311, such that the upper side of the water absorption pad 312 is flush with the upper edge of the PVC plate 311, and the lower edge of the water absorption pad 312 is pressed to the membrane strip 3132 mm; the colloidal gold pad 314 is stuck below the PVC plate 311, the upper film pressing strip 3133 mm of the colloidal gold pad 314 protrudes below the PVC plate 3115 mm.

Certainly, the structure of the test strip 3 or the test strip 4 is not limited to the structure shown in fig. 5, and may also be some other existing test strip structures, or only include a membrane strip and a colloidal gold pad, and the test strips may share the colloidal gold pad or each test strip has a separately configured colloidal gold pad. The specific specification of the test strip is not limited to this, and can be adjusted according to actual needs.

In some embodiments, the membrane strip 313 is a nitrocellulose membrane.

In some embodiments, the test strip further comprises a PE film, wherein the upper edge of the PE film is attached to the nitrocellulose membrane along 3mm above the colloidal gold pad, and the lower side of the PE film is flush with the colloidal gold pad. The PE film has the waterproof function besides the function of connecting the colloidal gold pad, the nitrocellulose film and the colloidal gold pad.

In one embodiment, the assembly method of the colloidal gold detection reagent card comprises the following steps: a test strip structure (e.g., a test strip 3 or 4 according to the present disclosure) is matingly placed over detents 101 and 102 of lower cover 1, wherein one end of the test strip (the colloidal gold pad end) is flush with (abuts against) one end of second raised portion 106, contacting the recess. A blood filter pad is placed at the first sample adding hole 203 of the upper cover 2, and a sample pad is placed above the blood filter pad, so that in the assembled reagent card, the sample pad is stacked on the test strip and covers the part positions of the colloidal gold pad 314 and the membrane strip 313 on the test strip, wherein one end of the sample pad, which is in contact with the membrane strip 313, protrudes 2mm out of the PE membrane covered on the membrane strip 313 and is abutted to the membrane strip 313 (the position which is not covered by the PE) in the test strip. And then the upper cover 1 and the lower cover 2 are buckled together. Wherein, because of the existence of the PE film, the liquid in the sample pad can directly chromatographically flow on the film strip, and the liquid can not touch the colloidal gold pad in the process, thereby achieving the purpose of detection.

In some embodiments, the reagent card is used for detecting an allergen, and the specific operation is to add a sample from the first sample adding hole 203, for example, the sample is finger blood, which is filtered through a blood filter pad after being added from the first sample adding hole 203, and then the sample flows through the sample pad; because the sample pad abuts the membrane strip in the test strip, the sample flows to the membrane strip. The developing solution is added from the second sample adding hole 204, and the colloidal gold compound flows to the membrane strip through the end part of the colloidal gold pad; finally, an antigen-antibody complex is formed on the membrane strip, thereby achieving the purpose of detection. It should be understood that there may be one gold gel per membrane strip during the arrangement, and as shown in fig. 3 in particular, the developing solution is added from the second sample adding hole 204, falls into the first groove 101, then slowly flows to the left and right slits through the first groove, and then contacts with the gold gel pads arranged on the left and right membrane strips after passing through the slits, so as to bring the gold gel compound into the membrane strips. Or a plurality of membrane strips correspond to a colloidal gold phase during setting, specifically, the developing solution falls into the first groove 101 after being added from the second sample adding hole 204, and then the liquid flows into or permeates into the colloidal gold through the pipeline or the paper strip to capture the colloidal gold compound therein and then flows to each membrane strip through the pipeline or the paper strip.

In some embodiments, the assembly method of the colloidal gold detection reagent card is as follows: a blood filter pad is placed in the first well 203 of the upper lid 2, and a sample pad is placed above the blood filter pad so that the sample pad abuts against the lower end of the gold gel pad 314 in the assembled reagent card. So that the liquid in the sample pad can be directly chromatographed on the colloidal gold pad 314, and then continuously chromatographed in the membrane strip through the colloidal gold pad, thereby achieving the purpose of detection. The use of a blood filter pad can be applied to the detection of samples such as interphalangeal blood and venous blood that are prone to background interference.

In some examples, two first bosses 205 may be covered by one sample pad at the same time, with the blood filter pad in the center of the sample pad. The test strip 3 or the test strip 4 is placed on the screens 102 of the lower cover 1. After all the materials are put, the lower cover 1 is buckled on the upper cover 2, and the two are tightly pressed, so that a complete test paper card is formed.

In some embodiments, the reagent card is used to detect allergens by adding a sample from a first well 203 and a developing solution from a second well. For example, the sample is interphalangeal blood, which is filtered through a blood filter pad after being introduced through the first introduction hole 203, and then the sample flows through the sample pad. Because the sample pad abuts against the end of the colloidal gold pad 314 in the test strip, the sample flows from the sample pad to the colloidal gold pad in the test strip and then to the membrane strip, thereby achieving the purpose of detection. Depending on the test strips and the particular requirements, a liquid, such as a developing liquid, may be added to the second well 204 after the sample has been reacted through the first well 203 for a period of time.

In one embodiment, in testing a sample with a reagent card of the present disclosure, the steps of operating include:

(1) adding 100uL of fingertip blood, whole blood or serum to be detected into a first sample adding hole 203 of the reagent card, and timing for 5 min;

(2) adding 0.5mL of the development solution into the second sample adding hole 204, and timing for 15 min;

(3) and after the reaction time of the reagent card is over, the reagent card is placed in a colloidal gold card reader to read the numerical value, and the sample allergic concentration is automatically calculated and classified by the instrument according to the batch of kit standard curves.

Of course, the test reagent card of the present disclosure is not limited to the detection of allergens. Can be used for detecting virus, drugs and the like. The test sample is not limited to interphalangeal blood, and the test sample can be selected from saliva, whole blood, interphalangeal blood, serum or urine.

The reagent card can simultaneously detect a plurality of test strips through one sample adding hole, can save the sample using amount and improve the detection efficiency, and can be suitable for detecting samples which are easy to generate background interference, such as interphalangeal blood and venous blood, and has better practicability because the blood filtering pad is arranged in the reagent card.

In addition, the reagent card disclosed by the invention can also comprise a plurality of sample adding holes, so that one reagent card can be used for simultaneously detecting a plurality of groups of samples, and the detection efficiency is greatly improved.

The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention cannot be limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are all within the protection scope of the present invention.

Claims (34)

1. The utility model provides a reagent card, its characterized in that, includes lid and membrane strip, colloidal gold and connecting piece, and membrane strip, colloidal gold and connecting piece are all established in the lid is internal, the lid is equipped with first application of sample hole and second application of sample hole, first application of sample hole is corresponding with the membrane strip, second application of sample hole corresponds with the colloidal gold, the connecting piece is used for connecting membrane strip and colloidal gold pad.

2. The reagent card of claim 1, wherein liquid passing through the colloidal gold pad flows onto the membrane strip through the connector.

3. The reagent card of claim 1, wherein the colloidal gold pad is positioned higher than the membrane strip.

4. The reagent card of claim 1, wherein the connecting member is a membrane strip structure, the gold gel pad is disposed on one side of the connecting member, and the membrane strip is disposed on the other side of the connecting member.

5. The reagent card of claim 1 wherein the connector is the same material as the membrane strip.

6. The reagent card of claim 1 wherein the connector is integrally formed with the membrane strip.

7. The reagent card of claim 4, further comprising a sample pad; the colloidal gold pad is placed at one end of the membrane strip, one end of the sample pad is abutted to the membrane strip, and the other end of the sample pad corresponds to the first sample adding hole.

8. The reagent card of claim 7, wherein the reagent card comprises a plurality of membrane strips, the cover has a plurality of viewing windows in one-to-one correspondence with the membrane strips, and the membrane strips are connected to each other.

9. The reagent card of claim 4, wherein the first loading well corresponds to at least two membrane strips.

10. The reagent card of claim 1, wherein at least two membrane strips are connected to each other, the connection of the interconnected membrane strips being a first connection center, the first well corresponding to the position of the first connection center.

11. The reagent card of claim 10 wherein the junctions of all the interconnected membrane strips are the same.

12. The reagent card of claim 1, wherein all of the membrane strips are connected to each other.

13. The reagent card of claim 10 wherein at least two of said membrane strips are of different types.

14. The reagent card of claim 10 wherein the coating on at least two of the membrane strips is different.

15. The reagent card of claim 10 wherein the coating is different on all of the membrane strips.

16. The reagent card of claim 1, wherein one membrane strip corresponds to one colloidal gold.

17. The reagent card of claim 1, wherein one colloidal gold corresponds to a plurality of membrane strips.

18. The reagent card of claim 1, further comprising a bibulous pad.

19. The reagent card of claim 18, further comprising a PVC plate.

20. The reagent card of claim 18, wherein the colloidal gold pad, the membrane strip and the absorbent pad are sequentially laid on the PVC plate, the absorbent pad is placed at one end of the membrane strip, and the colloidal gold pad is placed at the other end of the membrane strip.

21. The reagent card of claim 20, wherein the reagent card is further covered with a waterproof membrane.

22. The reagent card of claim 21, wherein the waterproof membrane is a PE membrane.

23. The reagent card of claim 22, wherein the PE film is covered over the film strip and the colloidal gold pad.

24. The reagent card of claim 22, wherein one end of the PE film is 2-3mm beyond one end of the colloidal gold pad and the other end of the PE film is flush with the other end of the colloidal gold pad.

25. The reagent card of claim 7, further comprising a blood filter pad disposed within the cover.

26. The reagent card of claim 25, wherein the blood filter pad is positioned below the first well and abuts the sample pad.

27. The reagent card of claim 8, wherein the cover comprises an upper cover and a lower cover.

28. The reagent card of claim 27, wherein the upper cover is provided with a first protrusion, the observation window and the first well are provided on the upper cover, the first protrusion is located at one end of the observation window, and the first protrusion is located at both sides of the first well, and the first protrusion is used for placing the sample pad.

29. The reagent card of claim 25, wherein the sample pad has an area larger than the area of the blood filter pad and is placed on the blood filter pad.

30. The reagent card of claim 27, wherein the lower cover is provided with a detent for placing the membrane strip and the colloidal gold.

31. The reagent card of claim 30, wherein the size of the card matches the size of the membrane strip and colloidal gold.

32. The reagent card of claim 30, wherein a second protrusion is provided between the two screens at an end near the first well for separating the membrane strip and the gold gel at both sides.

33. The reagent card of claim 27, wherein the lower cover is provided with more than 1 groove corresponding to the second well, and the groove is connected with the colloidal gold through a pipe or a paper slip.

34. The reagent card of claim 33, wherein there are more than 2 recesses, including a first recess disposed directly below the second well and a second recess disposed between the first recess and the card.

Images