CN213113358U

CN213113358U - Biological culture unit device

Info

Publication number: CN213113358U
Application number: CN202020324207.5U
Authority: CN
Inventors: 欧阳宏伟; 张靖微; 廖友国; 吴心宇
Original assignee: Zhejiang University ZJU
Current assignee: Zhejiang University ZJU
Priority date: 2020-03-16
Filing date: 2020-03-16
Publication date: 2021-05-04
Anticipated expiration: 2030-03-16

Abstract

The utility model provides a novel biological culture unit device, including cultivateing the chamber, the oral area of cultivateing the chamber is equipped with the shutoff part, be equipped with on the shutoff part and stretch into cultivate the pipe in chamber, be equipped with at least one deck diaphragm in the cavity of cultivateing the chamber, be equipped with at least one via hole on the diaphragm, can allow the pipe passes. It is possible to provide some means for solving the problems in the background art. The utility model discloses a novel biological culture unit device can be used for carrying out the biological process operation of cultivateing the class such as cell culture, and this biological culture unit device can regard as the independent culture unit that is used for cultivateing the cell among many cell reaction unit, can also set up at least one deck in this biological culture unit device and cultivate the diaphragm, keeps apart into different cavities with the cell culture unit for found suitable cultivation environment, cultivate respectively or compare the cultivation.

Description

Biological culture unit device

Technical Field

The utility model relates to the field of biotechnology, especially, relate to a device for biological cultivation.

Background

Biological cultivation is widely used in biotechnology field, generally speaking, a biological cultivation device needs to have at least one experimental container capable of accommodating biomass for growth, propagation and other operations, and this experimental container may also perform some other cultivation operations according to experimental settings under required conditions, such as centrifugation, mixing, sampling, freezing and the like.

Disclosure of Invention

The utility model aims to solve the technical problem of providing a novel biological culture unit device, which can provide some solving ways aiming at the problems in the background technology.

The utility model provides a technical scheme that above-mentioned technical problem adopted is:

a biological growth unit device comprising a growth chamber including at least one membrane separating the growth chamber into at least two growth areas, the membrane being generally disposed within the growth chamber and being relatively fixed in position within the growth chamber.

Further, the membrane sheet comprises a semi-permeable membrane, and the membrane sheet can be a semi-permeable membrane, a fully permeable membrane or an impermeable membrane, and can also be a combination of the membranes.

Furthermore, the membrane is provided with a plurality of through holes which are distributed on the membrane, and the aperture of each through hole can be 30 μm-1mm, so that the membrane can form a semi-permeable membrane state, can allow a part of substances to permeate through the membrane, and simultaneously block a part of substances.

Further, the culture chamber adopts a biosafety container and/or a biochip. In some modes, the culture cavity can adopt a container meeting the biosafety requirement, such as a glass culture dish, a centrifuge tube and the like, and in some modes, the culture cavity can adopt a biochip which is more commonly used at present, so that the culture cavity is better in adaptability.

Further, the culture regions can be independent or in communication with each other. In some embodiments, the membranes may be impermeable to separate the culture regions to form separate culture chambers, in other embodiments, the membranes may be semi-permeable to partially separate the culture regions, or in other embodiments, the membranes may be permeable to reduce the flow rate of the culture medium or culture medium through the pores of the membranes.

Further, the culture cavity comprises a liquid inlet and a liquid outlet, and can be used for inputting and outputting culture solution and/or culture substances.

Further, the culture cavity includes the liquid taking port, and in some modes, can get the liquid through the liquid taking pipe, then the liquid taking port can include the liquid taking pipe this moment, and the liquid taking port can stretch into the culture cavity for sampling when carrying out the cultivation etc. in some modes, the liquid taking port can directly set up on certain cultivation region.

Further, the membrane comprises a membrane support for supporting, the membrane support can be used for supporting and fixing the membrane, the membrane support can also be made of a biological safety material, such as biological safety plastic or plastic material, and the like, the membrane support can clamp and fix the membrane, and then the membrane support is further fixed on the inner wall of the culture cavity, so that the membrane is fixed.

Further, the membrane support comprises at least one area for installing the membrane, and in some modes, the membrane support can be divided into a plurality of areas, so that on one hand, the fixation of the membrane can be reinforced, on the other hand, the expansibility of the membrane can be better, for example, different types of membranes can be respectively placed in different areas of the membrane support to be matched for use, and the effect of comprehensive utilization can be achieved.

Further, the culture cavity comprises a blocking component which is used for closing the culture cavity to form a closed culture space, so that pollution is avoided, and liquid leakage is prevented.

The utility model has the advantages that: the utility model discloses a novel biological culture unit device can be used for carrying out the biological process operation of cultivateing the class such as cell culture, and this biological culture unit device can regard as the independent culture unit that is used for cultivateing the cell among many cell reaction unit, can also set up at least one deck in this biological culture unit device and cultivate the diaphragm, keeps apart into different cavities with the cell culture unit for cultivate respectively or compare the cultivation.

Drawings

Fig. 1 is an overall configuration diagram of embodiment 1.

Fig. 2 is a schematic view of the first carriage ring of embodiment 1.

Fig. 3 is a schematic view of a second stent ring of embodiment 1.

Fig. 4 is a schematic view of the retainer ring of embodiment 1.

Fig. 5 is a partially enlarged view of the diaphragm of example 1.

FIG. 6 is a schematic view of the entire structure of embodiment 2.

FIG. 7 is a schematic view of the entire structure of embodiment 3.

FIG. 8 is a schematic view of the entire structure of embodiment 4.

Reference numbers in the figures:

example 1: culture chamber 1, culture area 101, shutoff part 2, feed liquor pipe 3, drain pipe 4, sampling tube 5, diaphragm 6, via hole 61, pore 7, support ring 8, retainer plate 9, pipe support 10, support frame 11, pore position 12, support position 13, space 14.

Example 2: liquid inlet layer 17, liquid inlet 171, culture layer 18, liquid outlet layer 19 and liquid outlet 191.

Example 3: a liquid inlet 15, a liquid outlet 16, a first culture area 102, a second culture area 103 and a third culture area 104.

Example 4: first culture vessel 106, second culture vessel 107, third culture vessel 108, and cover plate 109.

Detailed Description

The following detailed description of the embodiments of the present invention will be made with reference to the accompanying drawings, and it should be noted that the embodiments are only for illustrating the present invention in detail, and the purpose thereof is to enable those skilled in the art to better understand the technical solution of the present invention, and should not be construed as limiting the present invention.

Example 1, a tube culture chamber was centrifuged, see FIGS. 1-5.

As shown in the figure, the utility model provides a biological culture unit device, including cultivateing chamber 1, cultivate chamber 1 and can adopt the centrifuging tube as shown in the figure, also can be ordinary test tube or have the similar testing device of certain pipe diameter, cultivate the transparent body that chamber 1 can adopt glass or plastic materials to make.

Cultivate the oral area in chamber 1 and be equipped with plugging member 2, plugging member 2 can adopt cork or rubber buffer, be equipped with on plugging member 2 and stretch into cultivate the inside pipe in chamber 1, the pipe includes feed liquor pipe 3, drain pipe 4 and sampling tube 5, feed liquor pipe 3 stretches into and is close cultivate the position of the bottom in chamber, generally speaking, feed liquor pipe 3 is close the bottom better more, but do not touch the bottom, will leave 1-5mm play liquid distance, avoid blockking up, the oral area of drain pipe 4 is close to the bottom of plugging member 2, is close the position of the last liquid level of culture solution, generally speaking, perhaps breaks the certain time after the cultivation is accomplished, carries out the flowing back. The sampling tube 5 extends into the middle part of the culture cavity 1 and is used for extracting culture substances.

The culture cavity 1 is internally provided with a diaphragm 6, in the embodiment of taking the centrifugal tube as the culture cavity 1, preferably, the diaphragm 6 is perpendicular to the height direction of the culture cavity 1, and simply, the diaphragm 6 can be transversely placed in the culture cavity 1 to separate the culture cavity 1 into different culture spaces. Preferably, an impermeable membrane, a semi-permeable membrane or a fully permeable membrane can be placed on the membrane, and the membrane is selected as required, when the fully permeable membrane is placed, the culture chamber 1 can be divided into different culture areas 101, in some cases, scales can be arranged on the wall of the culture chamber 1, the placement position of the membrane 6 can be adjusted, and the culture space can be determined according to the scales; when the semipermeable membrane is placed, selective permeation can be realized, and culture of different levels can be realized; when the perm-membrane is placed, the flow rate of the culture solution into the next culture space can be adjusted. For example, as shown in fig. 5, a plurality of through holes 61 may be disposed on the membrane 6, and the through holes 61 may be distributed on the membrane 6, and the aperture of the through holes 61 may be 30 μm to 1mm, so that the membrane 6 may form a semi-permeable membrane state, which allows a portion of the substance to permeate therethrough, and blocks a portion of the substance.

Preferably, the membrane 6 may be supported by a membrane support, the membrane support includes a support ring 8 and a fixing ring 9, the support ring 8 is made of plastic or plastic material, the support ring 8 includes a tube support 10 and a support frame 11, the tube support 10 is provided with the at least one tube hole 7, the support frame 11 is in accordance with the shape of the inner wall of the culture chamber 1, for example, when the culture chamber 1 has a circular cross section, the support frame 11 has a circular shape, when the culture chamber 1 has a square shape, the support frame 11 has a square shape, so as to ensure that the support frame 11 can be supported on the cross section of the culture chamber 1, and the periphery of the support frame 11 may be sleeved with a sealing ring for fixing and sealing the periphery.

Preferably, the pipe bracket 10 includes a pipe hole portion 12 and a bracket portion 13, the pipe hole 7 is provided in the pipe hole portion 12, and a sealing ring may be fitted around a pipe passing through the pipe hole 7 to seal a gap between the pipe hole 7 and an outer wall of the pipe to prevent leakage. The supporting portions 13 are diverged or uniformly distributed between the tube hole portion 12 and the supporting frame 11 for connecting the two, for example, in the embodiment, the supporting portions 13 are cross-shaped supporting portions and uniformly distributed in the space between the tube hole portion 12 and the supporting frame 11, accordingly, a gap 14 is formed between the supporting portions 13, and this gap 14 can be filled by the above-mentioned impermeable film, semi-permeable film or fully-permeable film, for example, when the supporting frame is manufactured, the impermeable film, semi-permeable film or fully-permeable film is adhered to the supporting portions 13 and the supporting frame 11, and in some modes, different types of membranes can be filled in different gaps 14 according to needs.

Preferably, the retainer plate 9 can adopt the silica gel material to make, the retainer plate 9 can be established by the cover the periphery of support frame 11, and the retainer plate that makes with the silica gel material can adsorb on the inner wall of cultivateing the chamber through frictional force to fix the diaphragm on the inner wall of cultivateing the chamber, in some preferred modes, the retainer plate 9 is fixed in the periphery of support frame 11 through the elastic force of self, and in some preferred modes, support frame 11 peripheral upper and lower both ends can set up the arch, with the spacing wherein of retainer plate 9.

Preferably, as shown in this embodiment, the membrane 6 may comprise two pieces, and when the membrane is two pieces, they are disposed in the culture chamber 1 at a certain distance, so that the culture chamber 1 can be divided into more spaces.

Preferably, generally, there are more through holes on the upper membrane than on the lower membrane, for example, when there are two through holes on the upper membrane, the lower membrane may have only one through hole, the two through holes of the upper membrane may be used for passing through the liquid inlet pipe and the sampling pipe, and the through hole of the lower membrane only needs to pass through the liquid inlet pipe, in this case, we may refer to the bracket ring in the upper membrane as the first bracket ring, there are two pipe holes 7 on the through hole portion 13 of the first bracket ring for passing through the liquid inlet pipe 3 and the sampling pipe 5, we may refer to the bracket ring in the lower membrane as the second bracket ring, there is one pipe hole 7 on the through hole portion 13 of the second bracket ring for passing through the sampling pipe 5. Of course, the utility model discloses also do not get rid of the condition of a plurality of feed liquor pipes, a plurality of sampling tubes, multilayer diaphragm, can set up as required, for example can adopt a plurality of sampling tubes, stretch into respectively and take a sample in the middle of the different cultivation layers.

Example 2, biochip, see FIG. 6.

As shown in fig. 6, the culture chamber 1 may be a biochip, which includes a liquid inlet layer 17, a culture layer 18, and a liquid outlet layer 19, wherein the culture layer 18 is located between the liquid inlet layer 17 and the liquid outlet layer 19, wherein a plurality of liquid inlets 171 (e.g., 3 in the figure) are disposed on the liquid inlet layer 17, and the liquid inlet layer 171 can be filled with liquid for culture, and the like, at least one liquid outlet 191 (e.g., 2 in the figure) is disposed on the liquid outlet layer 19, and membranes 6 may be disposed in the liquid inlet layer 17, the culture layer 18, and the liquid outlet layer 19, in this embodiment, the membranes 6 may not employ membrane holders.

Other embodiments of this example are the same as example 1.

Example 3, biosafety container 1, see figure 7.

As shown in FIG. 7, the culture chamber 1 may be a container made of biosafety material, the volume of the container may be 10 μ L-500 mL, the upper portion of the container is provided with a liquid inlet 15, the lower portion of the container is provided with a liquid outlet 16, and in some other ways, the positions of the liquid inlet 15 and the liquid outlet 16 may be interchanged according to the connection manner of the liquid inlet and the liquid outlet.

Three different culture areas 101 are arranged in the container, the culture areas 101 can be separated by membranes 6, the liquid inlet 15 is communicated with a first culture area 102, the first culture area 102 is cylindrical as a whole and has a smaller inner diameter, preferably, a layer of membrane 6 can be arranged between the liquid inlet 15 and the first culture area 102 for filtering or separation, preferably, a transition area with gradually-increased caliber is arranged between the liquid inlet 15 and the first culture area 102 for introducing liquid, the first culture area 102 is respectively communicated with a plurality of (3 in the embodiment) second culture areas 103 in different directions after one section, the membranes 6 can be arranged between the second culture areas 103 and the first culture area 102 for separating different areas, and the membranes 6 can be selected from semipermeable membranes, impermeable membranes or completely permeable membranes according to requirements, a third culture area 104 is arranged below the second culture area 103, a plurality of second culture areas 103 can be structurally communicated with the third culture area 104, a diaphragm 6 can be arranged at the junction of the second culture areas 103 and the third culture areas 104, similarly, the diaphragm 6 can also be a semi-permeable membrane, an impermeable membrane or a full-permeable membrane according to needs, and the lower part of the third culture area 104 is communicated with the liquid outlet 16, thus forming the integral structure of the whole culture cavity 1.

It should be noted that, the diaphragm installation mode in the culture chamber in this embodiment can be realized with reference to the mode in embodiment 1, and the culture chamber in this embodiment can be horizontally placed, vertically placed or obliquely placed as required, the present invention is not limited to its direction and application scenario.

Other embodiments of this example are the same as those of the example.

Example 4, biosafety container 1, see figure 8.

As shown in FIG. 8, the culture chamber 1 may be a container made of a biosafety material, the container may have a volume of 10. mu.L-500 mL, the container may include a plurality of culture sections independent of each other, for example, a split design, the container may include a first culture container 106 at the bottom, a second culture container 107 capable of being accommodated in the first culture container 106, and a third culture container 108 on the second culture container 107, the second culture container 107 and the third culture container 108 may be connected integrally and internally, and a cover plate 109 may be provided outside the second culture container 107 and the third culture container 108, and when the second culture container 107 is put into the first culture container 106, the cover plate 109 may cover the mouth of the first culture container 106 to close the first culture container 106.

Furthermore, the first culture vessel 106 and the third culture vessel 108 are provided with a liquid inlet 15 and a liquid outlet 16, respectively, and the liquid inlet 15 and the liquid outlet 16 are separated from the culture spaces of the first culture vessel 106 and the third culture vessel 108 by a membrane 6, respectively.

In this embodiment, the diaphragm 6 may have the same inner diameter as the liquid inlet 15 and the liquid outlet 16, and the diaphragm 6 may have the structure of embodiment 1.

Other embodiments of this example are the same as example 1.

The above embodiments are only for the purpose of illustrating the different application modes of the present invention, and should not be considered as limiting the present invention, it should be noted that the specific embodiments of the present invention can also have other application modes except the several modes described by the embodiments, and all the scope covered by the claims of the present invention should be considered as the protection scope of the present invention.

Claims

1. The utility model provides a biological culture unit device, is characterized by including cultivateing the chamber, it includes at least one deck diaphragm to cultivate the chamber, the diaphragm can with it is regional for two at least cultivates that the chamber is kept apart to cultivate, the diaphragm includes the pellicle.

2. A biological growth cell arrangement according to claim 1, characterised in that the membrane has a through-going hole.

3. A biological growth cell arrangement according to claim 1, wherein the growth chamber is a biosafety container and/or biochip.

4. A biological culture unit arrangement according to claim 1, characterised in that the culture zones are independent or in communication with each other.

5. A biological growth element assembly according to claim 1, characterised in that the growth chamber includes a liquid inlet and a liquid outlet.

6. A biological growth cell device according to claim 1, characterised in that the growth chamber includes a liquid withdrawal port.

7. A biological culture unit according to claim 1, characterised in that the membrane comprises a membrane support for supporting.

8. A biological culture unit according to claim 7, characterised in that the membrane support includes at least one region for mounting a membrane sheet.

9. A biological culture unit arrangement according to claim 1, characterised in that the culture chamber comprises a closure member for closing the culture chamber.