CN213037687U - Device for extracting flavone from dendrobium officinale leaves - Google Patents
Device for extracting flavone from dendrobium officinale leaves Download PDFInfo
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- CN213037687U CN213037687U CN202021855007.9U CN202021855007U CN213037687U CN 213037687 U CN213037687 U CN 213037687U CN 202021855007 U CN202021855007 U CN 202021855007U CN 213037687 U CN213037687 U CN 213037687U
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Abstract
The utility model relates to a device for extracting flavone from dendrobium officinale leaves, which comprises an extraction system, an alcohol precipitation system, a distillation system, a sample loading system and a resin column system which are sequentially communicated through a pipeline, and further comprises a control system which is respectively in communication control connection with the extraction system, the alcohol precipitation system, the distillation system, the sample loading system and the resin column system, wherein a temperature control jacket in communication connection with the control system is sleeved outside both the extraction system and the distillation system which are arranged on a weighing device; the utility model provides an extract flavone's device in dendrobii officmalis caulis leaf provides a dendrobii officmalis caulis leaf flavone industrial preparation device.
Description
Technical Field
The utility model relates to a natural active ingredient separation and purification technical field among the medicinal plant especially relates to a device that draws flavone in dendrobii officmalis caulis leaf.
Background
From the end of the nineties of the last century, plant extracts are more and more concerned by the medical and food fields, become an important link for promoting the modernization of traditional Chinese medicines and the technical innovation of traditional Chinese medicines, are also an effective way for traditional Chinese medicines to enter the international market, and the plant extract industry has become one of the fastest-developing industries in China.
The traditional Chinese medicine plant extract belongs to an intermediate product in general, has wide application at present, and is mainly used as a raw material or an auxiliary material of health care products, functional foods, tobaccos and cosmetics. Dendrobium officinale is a rare traditional Chinese medicine, has a long history of medication, and is often used as a medicine by a stem. The leaves of Dendrobii herba belong to non-medicinal part, and most of the leaves are discarded. Researches in recent years show that the dendrobium officinale leaves are rich in natural plant active ingredients such as flavone, polysaccharide, alkaloid and the like. And the flavonoid compounds in the dendrobium have obvious anti-inflammatory, antibacterial and antioxidant activities, and have certain effects on preventing atherosclerosis and inhibiting cancer cell proliferation and canceration. Has better application potential in the fields of medicines, health products, cosmetics and the like. The related flavonoid extraction methods reported at present include a solvent extraction method, a microwave extraction method, an enzymatic hydrolysis method, a supercritical fluid extraction method, a two-aqueous phase extraction method, a semi-bionic extraction method, an ultrasonic method and the like, which are basically tests at a laboratory level, and no examples of large-scale production of the dendrobium leaf flavonoid are available so far. The extraction scale of a common laboratory is small, the automation degree is low, continuous preparation cannot be realized, and a certain distance is left from scale and productization. The extraction process has more influencing factors, and the quality uniformity and stability of each batch of extracted products are difficult to ensure only by manual control.
To solve the problems, the utility model provides a device for extracting flavone from dendrobium officinale leaves, which solves the problem that the existing equipment and process are not beneficial to the scale and quality of product preparation.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model aims at providing an extract flavone device in dendrobii officmalis caulis leaf suitable for industrial production.
The utility model discloses a device for extracting flavone from dendrobium officinale leaves, which comprises an extraction system, an alcohol precipitation system, a distillation system, a sample loading system and a resin column system which are sequentially communicated through a pipeline, and further comprises a control system which is respectively in communication control connection with the extraction system, the alcohol precipitation system, the distillation system, the sample loading system and the resin column system, wherein a temperature control jacket which is in communication connection with the control system is sleeved outside the extraction system and the distillation system which are arranged on a weighing device;
a first stirring device is arranged in an extraction tank in the extraction system, a plurality of first liquid outlets provided with filter plates are further formed in the bottom surface of the extraction tank, and a group of ultrasonic transducers positioned in the extraction tank are further arranged on the bottom surface of the extraction tank;
the alcohol precipitation system comprises at least two alcohol precipitation tanks connected in parallel, and the bottom surface of each alcohol precipitation tank is provided with a plurality of second liquid outlets provided with filter plates;
the top of a distillation tank in the distillation system is communicated with a condensing device, the top of the condensing device is provided with a vacuum pumping interface communicated with a vacuum pump, and the bottom of the condensing device is also provided with a condensate outlet communicated with an ethanol recovery tank;
the sample loading system comprises at least two sample loading tanks connected in parallel, and the bottom surface of each sample loading tank is provided with a first pH sensor;
the resin column system comprises a resin column and a sample loading pump with a plurality of liquid inlet valve ports.
Further, all seted up pan feeding mouth, fluid infusion mouth/CIP and compressed air mouth on the top surface of drawing jar, alcohol precipitation jar, retort and the last appearance jar, the fluid infusion mouth/CIP who sets up on the top surface of drawing jar and last appearance jar still establishes ties there is sour/alkali lye to add the mouth, still include with fluid infusion mouth/CIP intercommunication and stretch into jar internal mechanism that sprays, each the equal fixed mounting in tank bottoms of drawing jar, alcohol precipitation jar, retort and last appearance jar has a tank bottoms valve, each all be equipped with the second agitating unit on the bottom surface of alcohol precipitation jar, retort and last appearance jar tank bottoms.
Furthermore, the first stirring device or the second stirring device is a paddle type or inclined blade type stirring device, the first stirring device comprises a stirring motor, a stirring shaft and a stirring head, one end of the stirring shaft penetrates through the top of the extraction tank and is connected with the stirring motor, the other end of the stirring shaft is connected with the stirring head positioned in the extraction tank, the spraying mechanism comprises two spraying heads respectively communicated with the liquid supplementing port/CIP, and a resin column in the resin column system is communicated with a sample loading system through a sample loading pump with at least five liquid inlet valve ports.
Still further, draw intercommunication between jar and two parallelly connected alcohol precipitation jars and pass through connecting tube and connect first liquid outlet and pan feeding mouth, the intercommunication between two parallelly connected alcohol precipitation jars and the retort passes through connecting tube and connects second liquid outlet and pan feeding mouth, intercommunication between retort and two parallelly connected sample loading jars passes through connecting tube and connects the tank bottoms valve and the pan feeding mouth of retort bottom, each all be equipped with on the connecting tube with the automatic valve that the control system communication is connected.
Further, the control system comprises a controller and a computer in communication control connection with the controller.
Furthermore, an online liquid alcohol concentration detector extending into the distillation retort is arranged on the bottom surface of the distillation retort.
Further, the top surface of drawing the jar has still been seted up and has been looked the mirror mouth, the bottom surface of drawing the jar is inlayed and is stretched into draw the inside second pH sensor and the temperature sensor of jar, it is equipped with the sample valve to inlay on the lateral wall of appearance jar.
Furthermore, the aperture of the plurality of filter plates positioned on the bottom surface of the extraction tank is between 80 and 120 microns, and the aperture of the plurality of filter plates positioned on the bottom surface of the alcohol precipitation tank is between 10 and 50 microns.
Borrow by above-mentioned scheme, the utility model discloses at least, have following advantage:
1. all the equipment instruments and the valves of the utility model can be controlled by the computer-controlled controller, namely PLC, in a unified way, so that the automatic operation of the whole process flow can be realized, the artificial participation is greatly reduced, and the product quality and the batch consistency can be ensured;
2. the utility model has the advantages of controllable preparation process, high extraction efficiency, environmental friendliness, and can realize the requirement of large-scale continuous preparation of flavonoid compounds, and better serve the industrial production in the future of the fine development of traditional Chinese medicines;
to sum up, adopt this device to carry out the extraction preparation of dendrobii officmalis caulis leaf flavone, degree of automation is high, and preparation process is simple effective, and the preparation process is controllable, and extraction efficiency is high, and is friendly to the environment, can realize the demand of flavonoid large-scale continuous preparation, has solved traditional extraction in-process simultaneously to a certain extent, and intensity of labour is big, artifical too many batch product purity inequality scheduling problem.
The above description is only an overview of the technical solution of the present invention, and in order to make the technical means of the present invention clearer and can be implemented according to the content of the description, the following detailed description is made with reference to the preferred embodiments of the present invention and accompanying drawings.
Drawings
Fig. 1 is a schematic structural diagram of the device for extracting flavone from dendrobium officinale leaves of the utility model.
Detailed Description
The following detailed description of the embodiments of the present invention is provided with reference to the accompanying drawings and examples. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.
The device for extracting flavone from dendrobium officinale leaves shown in fig. 1 comprises an extraction system 1, an alcohol precipitation system 2, a distillation system 3, a sample loading system 4 and a resin column system 5 which are sequentially communicated through a pipeline, and further comprises a control system 6 which is respectively in communication control connection with the extraction system 1, the alcohol precipitation system 2, the distillation system 3, the sample loading system 4 and the resin column system 5, wherein temperature control jackets 8 which are in communication connection with the control system 6 are respectively sleeved outside the extraction system 1 and the distillation system 3 which are arranged on a weighing device 7;
a first stirring device 1-2 is arranged in an extraction tank 1-1 in the extraction system 1, a plurality of first liquid outlets 1-3 provided with filter plates are also arranged on the bottom surface of the extraction tank 1-1, and a group of ultrasonic transducers 1-4 positioned in the extraction tank 1-1 are also arranged on the bottom surface of the extraction tank 1-1;
the alcohol precipitation system 2 comprises at least two alcohol precipitation tanks 2-1 which are connected in parallel, and a plurality of second liquid outlets 2-2 provided with filter plates are formed in the bottom surface of each alcohol precipitation tank 2-1;
the top of a distillation tank 3-1 in the distillation system 3 is communicated with a condensing device 3-2, the top of the condensing device 3-2 is provided with a vacuum pumping interface 3-4 communicated with a vacuum pump 3-3, and the bottom of the condensing device 3-2 is also provided with a condensate outlet 3-6 communicated with an ethanol recovery tank 3-5;
the sample loading system 4 comprises at least two sample loading tanks 4-1 which are connected in parallel, and the bottom surface of each sample loading tank 4-1 is provided with a first pH sensor 4-2;
the resin column system 5 comprises a resin column 5-1 and a sample loading pump 5-2 with a plurality of liquid inlet valve ports.
In the embodiment, the top surfaces of the extraction tank 1-1, the alcohol precipitation tank 2-1, the distillation tank 3-1 and the sample loading tank 4-1 are respectively provided with a material inlet 9, a liquid supplementing port/CIP 10 and a compressed air port 12, the liquid supplementing port/CIP 10 arranged on the top surfaces of the extraction tank 1-1 and the sample loading tank 4-1 are also connected in series with an acid/alkali liquor adding port 11, so that the feeding, supplementing solution or solvent or cleaning solution, the acid/alkali liquor adjusting addition and pressurization control discharging can be respectively realized through the material inlet 9, the liquid supplementing port/CIP 10, the acid/alkali liquor adding port 11 and the compressed air port 12, the spraying mechanism communicated with the liquid supplementing port/CIP 10 and extending into the tank body is further included, the inner wall of the tank body can be fully cleaned through the spraying mechanism when the cleaning solution is introduced, and each extraction tank 1-1, the alcohol precipitation tank 1, the distillation tank 1 and the sample loading tank 4-1, The tank bottoms of the alcohol precipitation tank 2-1, the distillation tank 3-1 and the sample loading tank 4-1 are fixedly provided with tank bottom valves 13, waste materials can be discharged through the tank bottom valves 13 or the tank body feeding of the next step of the process is realized, and the bottom surfaces of the tank bottoms of each of the alcohol precipitation tank 2-1, the distillation tank 3-1 and the sample loading tank 4-1 are provided with second stirring devices 14, so that materials accumulated at the tank bottoms can be stirred fully and uniformly.
In this embodiment, the first stirring device 1-2 or the second stirring device 14 is a paddle type or inclined blade type stirring device, the first stirring device 1-2 includes a stirring motor 1-2-1, a stirring shaft 1-2-2 and a stirring head 1-2-3, one end of the stirring shaft 1-2-2 passes through the top of the extraction tank 1-1 and is connected with the stirring motor 1-2-1, the other end of the stirring shaft 1-2-2 is connected with the stirring head 1-2-3 in the extraction tank 1-1, the spraying mechanism includes two spraying heads 15 respectively communicated with a liquid supplementing port/CIP 10, the resin column 5-1 in the resin column system 5 is communicated with the sample loading system 4 through a sample loading pump 5-2 with at least five liquid inlet ports, so that flow rate adjustment and pressure adjustment can be performed through the sample loading pump 5-2 with at least five liquid inlet valves And (6) detecting the force.
In the present embodiment, the control system 6 includes a controller 6-1 and a computer 6-2 in communication control connection with the controller 6-1 for automation.
In this embodiment, in order to strictly control the ethanol concentration in the distillation tank 3-1, an online liquid ethanol concentration detector 16 extending into the distillation tank 3-1 is disposed on the bottom surface of the distillation tank 3-1, and a sampling valve 4-3 is embedded on the side wall of the sample loading tank 4-1.
In the embodiment, in order to facilitate real-time observation of various conditions and conditions in the extraction tank body, the top surface of the extraction tank 1-1 is further provided with a viewing mirror opening 17, and the bottom surface of the extraction tank 1-1 is embedded with a second pH sensor 18 and a temperature sensor 19 which extend into the extraction tank 1-1.
In the embodiment, in order to discharge waste materials through the tank bottom valve 13 at the bottom of the extraction tank 1-1 and the alcohol precipitation tank 2-1, the waste materials are filtered through the filter plates at the first liquid outlet 1-3 and the second liquid outlet 2-2 and then fed into the tank body in the next process flow, the communication between the extraction tank 1-1 and the two parallel alcohol precipitation tanks 2-1 is connected with the first liquid outlet 1-3 and the material inlet 9 through a connecting pipeline, the communication between the two parallel alcohol precipitation tanks 2-1 and the distillation tank 3-1 is connected with the second liquid outlet 2-2 and the material inlet 9 through a connecting pipeline, the communication between the distillation tank 3-1 and the two parallel sample loading tanks 4-1 is connected with the tank bottom valve 13 and the material inlet 9 at the bottom of the distillation tank 3-1 through a connecting pipeline, and each connecting pipeline is provided with an automatic valve 20 in communication connection with the control system 6, in this way, automation can be achieved by the control system 6.
In the embodiment, in order to respectively realize coarse filtration and fine filtration, the pore diameters of a plurality of filter plates positioned on the bottom surface of the extraction tank 1-1 are between 80 and 120 μm, and the pore diameters of a plurality of filter plates positioned on the bottom surface of the alcohol precipitation tank 2-1 are between 10 and 50 μm.
The utility model provides a principle of extracting flavone device in dendrobii officmalis caulis leaf is as follows:
1. first, a crude extract was prepared by the extraction system 1:
firstly, adding crushed dendrobium leaf powder of 40-80 meshes into an extraction tank 1-1 from a feeding port 9 of a material on the top surface of the extraction tank 1-1, feeding back the weight of the material in the extraction tank 1-1 to a control system 6 according to a weighing device 7 outside the extraction system 1, opening a liquid supplementing port/CIP 10, adding an extracting agent (45% ethanol) according to the material-liquid ratio (1g/20ml), starting a first stirring device 1-2 in the extraction tank 1-1 for stirring, starting a temperature control jacket 8 for controlling the temperature, feeding back the pH value of the material liquid in the extraction tank 1-1 by a second pH sensor 18 when the temperature in the extraction tank 1-1 reaches a set temperature of 50 ℃, comparing the pH value with the set value of pH 6, and controlling the opening of an automatic valve 20 on a connecting pipeline for adding alkali liquid into an acid/alkali liquid adding port 11 or an automatic valve 20 on the connecting pipeline for adding acid liquid, after the pH value is stabilized to a set value and stabilized for a period of time, a 1% complex enzyme preparation is added through a fluid infusion port/CIP 10, wherein the cellulase: the method comprises the following steps of 1.5:1 of papain, carrying out enzymolysis for 60-90 min, enabling a tank body of an extraction tank 1-1 to enter an enzymolysis stage, automatically closing a temperature control jacket 8 after the enzymolysis time is finished, starting a group of ultrasonic transducers 1-4 to enter an ultrasonic extraction stage, carrying out ultrasonic crushing with the ultrasonic power of 120W and the ultrasonic time of 10 min.
After the ultrasound is finished, the PLC controller sends out an instruction to open an automatic valve 20 at a first liquid outlet 1-3 of the filter plate arranged on the bottom surface of the extraction tank 1-1, filling compressed air into the extracting tank 1-1 through a compressed air port 12, pressing the extracting solution into the alcohol precipitation tank 2-1 through a connecting pipeline connecting the extracting tank 1-1 and the alcohol precipitation tank 2-1, after a weighing device 7 outside the extracting tank 1-1 feeds back the weight of the extracting tank 1-1 to be constant, closing the automatic valve 20 at the compressed air port 12, closing the automatic valve 20 at the first liquid outlet 1-3, opening the automatic valve 20 at the opening of the liquid supplementing port/CIP 10 again, adding the extracting agent according to the material-liquid ratio (1g/10ml), entering the ultrasonic stage again, repeating the process, and pressing the secondary extracting solution into the alcohol precipitation tank 2-1. When the alcohol precipitation tank 2-1 reaches 1/3 with a set volume, the extract from the extraction tank 2-1 automatically switches to flow into another alcohol precipitation tank 2-1 connected in parallel by controlling the opening and closing of the automatic valve 20 by the PLC controller 6-1, at this time, the alcohol precipitation tank 2-1 goes to the next step 2, after the extraction of the extraction tank 1-1 is completed for two times, the tank bottom valve 13 at the bottom of the extraction tank 1-1 is opened, and the stages of residue discharge and tank body cleaning are carried out.
2. Secondly, carrying out alcohol precipitation and impurity removal through an alcohol precipitation system 2:
after the step 1 is finished, an online alcohol concentration detector 16 in an alcohol precipitation tank 2-1 detects the concentration of the ethanol in the extracting solution, an automatic valve 20 at a liquid supplementing port/CIP 10 at the top of the alcohol precipitation tank 2-1 is opened, a second stirring device 14 is started to stir, so that the concentration of the ethanol in the extracting solution is not lower than 80%, each valve and the second stirring device 14 are closed, standing is not less than 6h, after the standing is finished, a PLC controller controls to open the automatic valve 20 at a second liquid outlet 2-2 of which the bottom surface is provided with a filter plate, injecting compressed air into the tank body of the alcohol precipitation tank 2-1 through a compressed air port 12, pressing the extracting solution into the distillation tank 3-1 through a connecting pipeline connecting the alcohol precipitation tank 2-1 and the distillation tank 3-1, the automatic valves 20 at the compressed air port 12 and at the second liquid outlet 2-2 are closed.
3. Then, the alcohol is distilled off by a distillation system 3:
after the step 2 is finished, starting the vacuumizing operation of a vacuumizing interface 3-4 which is arranged at the top of the condensing device 3-2 and communicated with the vacuum pump 3-3, enabling the negative pressure in the tank to be 0.1Mpa, operating the condensing device 3-2, starting the second stirring device 14, controlling the temperature of the temperature control jacket 8 to be 50-60 ℃, detecting the volume of the solution in the tank by the weighing device 7, sequentially executing temperature control closing of the temperature control jacket 8 when the solution is condensed to 1/6-1/7 of the original volume, closing the vacuumizing vacuum pump 3-3, closing the second stirring device 14, closing the condensing device 3-2, controlling to open a tank bottom valve 13 at the bottom of the distilling tank 3-1 by a PLC (programmable logic controller) after standing for a period of time, opening compressed air to fill the tank 3-1 with compressed air through a compressed air port 12, and pressing the concentrated solution into the sample loading tank 4-plus material through a connecting pipeline connecting the distilling tank 3-1 and the sample loading tank 4-1 1, after the weighing device 7 outside the distillation tank 3-1 feeds back the weight of the distillation tank 3-1 to be constant, closing the automatic valve 20 at the compressed air port 12 and closing the tank bottom valve 13.
4. Then, the concentration and the pH value of the concentrated solution after distillation are adjusted through a sample loading system 4:
after the step 3 is finished, sampling and measuring the total flavone concentration of the solution in the sample loading tank 4-1 through a sampling valve 4-3 on the tank body wall of the sample loading tank 4-1, supplementing the solution or adding purified water to dilute the solution to a target concentration value through an automatic valve 20 at a solution supplementing port/CIP 10, slowly adjusting the measured concentration value to the target concentration value of 2.2g/ml, performing real-time feedback through a first pH sensor 4-2 in the sample loading tank 4-1, and adding acid solution or alkali solution into the sample loading tank 4-1 through an acid/alkali solution adding port 11 to adjust the pH value to 2.4.
5. Finally, the concentrated solution with the adjusted pH value and the target concentration passes through a resin column system 5:
pretreatment of a resin column 5-1, namely a macroporous resin column, in the resin column system 5: soaking D101 macroporous adsorbent resin in 95% ethanol for 24 hr, loading into column, continuously washing the column with 95% ethanol, collecting effluent, and measuring absorbance of the effluent at 254nm until the absorbance is identical to 95% ethanol absorbance value, which indicates that the resin has been treated.
After the step 4 is finished, injecting the obtained sample loading liquid into a resin column 5-1 through a sample loading pump 5-2 connected with a bottom valve 13 of a sample loading tank 4-1, and entering a treated macroporous resin column for separation and purification, wherein the macroporous resin purification conditions comprise: the volume of the sample loading liquid is 16BV, the sample loading rate is 3BV/h, the water elution volume is 4BV, the 80 percent ethanol elution rate is 1BV/h, and the elution volume is 3.25 BV.
Vacuum freeze drying or spray drying the eluate to obtain refined herba Dendrobii leaf flavone powder containing quercetin, rutin, apigenin and vitexin as main ingredients.
A method for extracting flavone from dendrobium officinale leaves is characterized by comprising the following steps:
1. extraction: adding the crushed dendrobium officinale leaves into an extraction tank, adding an extracting agent, adjusting the temperature in the extraction tank to be 45-60 ℃, adjusting the pH to be 5-7, adding a compound enzyme for enzymolysis for 60-90 min, and finally performing ultrasonic extraction for 5-15 min;
2. alcohol precipitation: discharging the extract obtained in the step 1 through a filter plate, conveying the extract to an alcohol precipitation tank through a connecting pipeline, adding industrial ethanol into the alcohol precipitation tank to enable the ethanol concentration of the extract to reach more than 80%, and standing;
3. and (3) distillation and concentration: discharging the alcohol precipitation solution obtained in the step 2 through a filter plate, conveying the liquid to a distillation retort through a connecting pipe, and carrying out reduced pressure distillation and concentration in the distillation retort to remove ethanol in the extracting solution;
4. adjusting the pH value: conveying the concentrated solution obtained in the step (3) to a sample loading tank through a tank bottom valve and a connecting pipe at the bottom of a distillation tank, and adjusting the concentration and the pH value of the extracting solution;
5. separation and purification: and (4) enabling the sample loading liquid obtained in the step (4) to pass through a resin column through a sample loading pump, separating and purifying through macroporous resin in the resin column, and then performing vacuum freeze drying or spray drying to obtain refined dendrobium leaf flavone powder.
In this embodiment, the particle size of the dendrobium officinale leaf powder in step 1 is 60-80 meshes, the extractant is an ethanol solution with the volume concentration of 35-50%, the complex enzyme comprises cellulase and papain, the mass ratio of the complex enzyme to the dendrobium officinale leaves is 1-2%, and the cellulase: papain is 1-2: 1, the ultrasonic power is 100-125W, and the aperture of a filter plate at the bottom of the extraction tank is 80-120 mu m;
in the step 2, the concentration of the industrial ethanol is 95 percent or 99 percent, and the standing time is not less than 6 hours;
in the step 3, the aperture of a filter plate at the bottom of the alcohol precipitation tank is 10-50 mu m, the reduced pressure distillation condition is-1 bar, the temperature is 50-60 ℃, and the concentration volume is 1/5-1/6 of the original volume;
in the step 4, adjusting the concentration of the extracting solution to be 1.4-2.6 g/ml, and adjusting the pH value of the extracting solution to be 1.8-3.0;
the conditions for passing through the macroporous resin in the step 5 comprise that the volume of a sample loading solution is 12-16 BV, the volume of an eluent is 80% ethanol, and the elution volume is 3.0-3.5 BV.
The utility model provides an extract flavone device in dendrobii officmalis caulis leaf can reach 1.86% to the extraction rate of flavonoid in dendrobii officmalis caulis leaf, finally obtains flavonoid content and can reach 193.95 mg/g.
In the description of the present invention, it is to be understood that CIP refers to the abbreviation CLEAN IN PLACE, and that CIP cleaning is introduced into almost all food, beverage and pharmaceutical plants, both without disassembling the production equipment and with a safe and automatic cleaning system with simple operating methods. CIP cleaning not only cleans the machine, but also controls microorganisms. CIP cleaning technology has been widely used in advanced food industry, such as Angel yeast in the leading enterprise of yeast industry, where a full set of CIP cleaning procedures is used to clean pipes and tanks.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
The above is only the preferred embodiment of the present invention, and is not intended to limit the present invention, it should be noted that, for those skilled in the art, a plurality of improvements and modifications can be made without departing from the technical principle of the present invention, and such improvements and modifications should also be considered as the protection scope of the present invention.
Claims (8)
1. The utility model provides an extract device of flavone in dendrobii officmalis caulis leaf which characterized in that: the device comprises an extraction system (1), an alcohol precipitation system (2), a distillation system (3), a sample loading system (4) and a resin column system (5) which are sequentially communicated through a pipeline, and further comprises a control system (6) which is respectively in communication control connection with the extraction system (1), the alcohol precipitation system (2), the distillation system (3), the sample loading system (4) and the resin column system (5), wherein temperature control jackets (8) which are in communication connection with the control system (6) are respectively sleeved outside the extraction system (1) and the distillation system (3) which are arranged on a weighing device (7);
a first stirring device (1-2) is arranged in an extraction tank (1-1) in the extraction system (1), a plurality of first liquid outlets (1-3) provided with filter plates are further formed in the bottom surface of the extraction tank (1-1), and a group of ultrasonic transducers (1-4) located in the extraction tank (1-1) are further arranged on the bottom surface of the extraction tank (1-1);
the alcohol precipitation system (2) comprises at least two alcohol precipitation tanks (2-1) connected in parallel, and a plurality of second liquid outlets (2-2) provided with filter plates are formed in the bottom surface of each alcohol precipitation tank (2-1);
the top of a distillation tank (3-1) in the distillation system (3) is communicated with a condensing device (3-2), the top of the condensing device (3-2) is provided with a vacuum pumping interface (3-4) communicated with a vacuum pump (3-3), and the bottom of the condensing device (3-2) is also provided with a condensate outlet (3-6) communicated with an ethanol recovery tank (3-5);
the sample loading system (4) comprises at least two sample loading tanks (4-1) connected in parallel, and the bottom surface of each sample loading tank (4-1) is provided with a first pH sensor (4-2);
the resin column system (5) comprises a resin column (5-1) and a sample loading pump (5-2) with a plurality of liquid inlet valve ports.
2. The device for extracting flavone from dendrobium officinale leaves as claimed in claim 1, wherein: the top surfaces of the extraction tank (1-1), the alcohol precipitation tank (2-1), the distillation tank (3-1) and the sample loading tank (4-1) are all provided with a feeding port (9), a liquid supplementing port/CIP (CIP) (10) and a compressed air port (12), draw liquid supplementing mouth/CIP (10) of seting up on the top surface of jar (1-1) and the appearance jar of going up (4-1) and still establish ties there is sour/alkali lye to add mouthful (11), still include with liquid supplementing mouth/CIP (10) intercommunication and stretch into jar internal spraying mechanism, each draw jar (1-1), alcohol precipitation jar (2-1), retort (3-1) and the equal fixed mounting in tank bottoms of the appearance jar of going up (4-1) tank bottoms has tank bottoms valve (13), each all be equipped with second agitating unit (14) on the bottom surface of alcohol precipitation jar (2-1), retort (3-1) and the appearance jar of going up (4-1) tank bottoms.
3. The device for extracting flavone from dendrobium officinale leaves as claimed in claim 2, wherein: the first stirring device (1-2) or the second stirring device (14) is a paddle type or inclined blade type stirring device, the first stirring device (1-2) comprises a stirring motor (1-2-1), a stirring shaft (1-2-2) and a stirring head (1-2-3), one end of the stirring shaft (1-2-2) penetrates through the top of the extraction tank (1-1) and is connected with the stirring motor (1-2-1), the other end of the stirring shaft (1-2-2) is connected with the stirring head (1-2-3) positioned in the extraction tank (1-1), the spraying mechanism comprises two spraying heads (15) respectively communicated with the liquid supplementing port/CIP (10), and a resin column (5-1) in the resin column system (5) is connected with a sample loading pump with at least five liquid inlet ports And (5-2) is communicated with the sample loading system (4).
4. The device for extracting flavone from dendrobium officinale leaves as claimed in claim 1, wherein: the control system (6) comprises a controller (6-1) and a computer (6-2) in communication control connection with the controller (6-1).
5. The device for extracting flavone from dendrobium officinale leaves as claimed in claim 1, wherein: an online liquid alcohol concentration detector (16) extending into the distillation retort (3-1) is arranged on the bottom surface of the distillation retort (3-1).
6. The device for extracting flavone from dendrobium officinale leaves as claimed in claim 1, wherein: the top surface of drawing jar (1-1) has still seted up and has looked mirror mouth (17), the bottom surface of drawing jar (1-1) is inlayed and is stretched into draw jar (1-1) inside second pH sensor (18) and temperature sensor (19), it is equipped with sample valve (4-3) to inlay on the lateral wall of appearance jar (4-1).
7. The device for extracting flavone from dendrobium officinale leaves as claimed in claim 2, wherein: draw intercommunication between jar (1-1) and two parallelly connected alcohol precipitation jar (2-1) and pass through connecting tube and connect first liquid outlet (1-3) and pan feeding mouth (9), intercommunication between two parallelly connected alcohol precipitation jar (2-1) and retort (3-1) passes through connecting tube and connects second liquid outlet (2-2) and pan feeding mouth (9), intercommunication between retort (3-1) and two parallelly connected sample loading jar (4-1) is connected through connecting tube tank bottoms valve (13) and pan feeding mouth (9) of retort (3-1) bottom, each all be equipped with on the connecting tube with automatic valve (20) that control system (6) communication is connected.
8. The device for extracting flavone from dendrobium officinale leaves as claimed in claim 1, wherein: the aperture of the plurality of filter plates positioned on the bottom surface of the extraction tank (1-1) is between 80 and 120 mu m, and the aperture of the plurality of filter plates positioned on the bottom surface of the alcohol precipitation tank (2-1) is between 10 and 50 mu m.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021855007.9U CN213037687U (en) | 2020-08-31 | 2020-08-31 | Device for extracting flavone from dendrobium officinale leaves |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021855007.9U CN213037687U (en) | 2020-08-31 | 2020-08-31 | Device for extracting flavone from dendrobium officinale leaves |
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| CN213037687U true CN213037687U (en) | 2021-04-23 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111909125A (en) * | 2020-08-31 | 2020-11-10 | 苏州市海崴生物科技有限公司 | Device and method for extracting flavone from dendrobium officinale leaves |
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2020
- 2020-08-31 CN CN202021855007.9U patent/CN213037687U/en not_active Withdrawn - After Issue
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111909125A (en) * | 2020-08-31 | 2020-11-10 | 苏州市海崴生物科技有限公司 | Device and method for extracting flavone from dendrobium officinale leaves |
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