CN213012781U

CN213012781U - DNA amplification device

Info

Publication number: CN213012781U
Application number: CN202020777690.2U
Authority: CN
Inventors: 李军委; 李姗姗; 潘峰; 孟冀豫
Original assignee: Hebei University of Technology
Current assignee: Hebei University of Technology
Priority date: 2020-05-12
Filing date: 2020-05-12
Publication date: 2021-04-20
Anticipated expiration: 2030-05-12

Abstract

The utility model relates to a DNA amplification device, the test platform comprises a rack, be fixed with a plurality of heating plates on the rack, a plurality of heating plates are the annular and arrange, and one side of every heating plate is fixed with temperature sensor, and the upper surface of every heating plate is fixed with the conducting strip respectively, has placed the chip on a plurality of conducting strips, and the inside spiral of chip has arranged many rings of microtubes.

Description

DNA amplification device

Technical Field

The utility model relates to a micro-fluidic technology field especially relates to a DNA amplification device.

Background

The principle of DNA amplification is:

the double-stranded DNA can be decomposed into two single-stranded DNA molecules at about 95 ℃, the single-stranded DNA and the primer are subjected to semi-reserved replication under the action of DNA polymerase at about 60 ℃, double DNA molecules can be obtained in one period, the temperature is controlled to be repeatedly circulated at 95 ℃ and 60 ℃, and the number of the DNA molecules can be exponentially increased. However, in the conventional DNA amplification, a temperature control module is usually used for controlling, a reagent containing DNA is placed in a container, and the temperature of the reagent in the container is repeatedly raised and lowered by controlling the temperature control module, so that the method is complicated and low in efficiency. DNA amplification is also partially realized by a microfluidic chip device with a special structure, but the device is of a constant section and cannot overcome the pressure drop existing in liquid flow.

SUMMERY OF THE UTILITY MODEL

The technical problem to be solved by the utility model is to overcome the defects existing in the prior art and provide a DNA amplification device.

The utility model discloses a realize through following technical scheme:

the utility model provides a DNA amplification device, includes the rack, be fixed with a plurality of heating plates on the rack, a plurality of heating plates are the annular and arrange, and one side of every heating plate is fixed with temperature sensor, and the upper surface of every heating plate is fixed with the conducting strip respectively, has placed the chip on a plurality of conducting strips, and the inside spiral of chip has arranged many circles microtubes.

Furthermore, the heat conducting fins are in a fan-shaped ring shape.

Furthermore, the cross sectional area of the outer ring of the micro pipeline is smaller than that of the inner ring of the micro pipeline.

The utility model has the advantages that:

inputting liquid containing DNA molecules from an inlet of the outer ring of the micro-pipeline through a liquid feeding device, and strictly controlling the flow feeding speed, wherein the micro-pipeline with the variable cross section is spirally arranged for N circles, and can be divided into four temperature zones which can be divided into four temperature zones, wherein the first temperature zone and the third temperature zone are denaturation zones, a temperature sensor is controlled in the denaturation zones to enable a heating piece unit to be stabilized at 95 ℃, and double-stranded DNA molecules are denatured and decomposed into two single-stranded DNA molecules in the denaturation zones; the second temperature zone and the fourth temperature zone are annealing zones, the temperature sensor is controlled in the annealing zones to enable the heating sheet unit to be stable at 60 ℃, in the annealing zones, single-stranded DNA molecules decomposed from double-stranded DNA molecules in the first temperature zone are combined with the primers, and half-retention replication is carried out under the action of DNA polymerase, so that the number of the DNA molecules is four times that of the original DNA molecules after one circle of liquid flows, and the DNA molecules can be cracked → combined → cracked → combined after one circle of liquid flows.

The temperature is stable and the temperature information of the heating plate is transmitted to the multi-channel temperature controller by the temperature sensor, so that the temperature of the heating plate is controlled to be realized, the trouble of repeated operation of temperature rise and temperature reduction is avoided as long as the preset temperature is stabilized, and the inflow speed is controlled.

Drawings

Fig. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic view showing the structure of the heat-conducting fin of the present invention;

FIG. 3 is a schematic view of the heating plate of the present invention;

FIG. 4 is a schematic diagram of the structure of the internal flow channel of the display chip of the present invention;

FIG. 5 is a schematic top view of the flow channel inside the chip of the present invention;

in the figure: 1. a chip; 2. a heat conductive sheet; 3. a heating plate; 4. a rack; 5. a temperature sensor; 6. A microchannel.

Detailed Description

In order to make those skilled in the art better understand the technical solution of the present invention, the present invention will be further described in detail with reference to the accompanying drawings and preferred embodiments.

As shown in the figure, the utility model discloses a rack 4, be fixed with a plurality of heating plates 3 on the rack 4, take four as examples in this embodiment, be N180 degrees between the central line at two adjacent heating plates 3 and the line at rack 4 center, one side of every heating plate 3 is fixed with temperature sensor 5, and the upper surface of every heating plate 3 is fixed with conducting strip 2 respectively, has placed chip 1 on four conducting strips 2, and the inside spiral of chip has arranged many rings of microtubes 6, conducting strip 2 is fan-shaped ring shape, conducting strip 2 is made by high coefficient of thermal conductivity material (like copper, aluminium, graphite alkene etc.).

The variable cross section micro pipeline 6 is spirally arranged for N circles, and can be divided into four temperature areas, wherein the first temperature area and the third temperature area are denaturation areas, the temperature sensor 5 is controlled in the denaturation areas to stabilize the unit of the heating plate 3 at 95 ℃, and double-stranded DNA molecules are denatured and decomposed into two single-stranded DNA molecules in the denaturation areas; the second temperature zone and the fourth temperature zone are annealing zones, the temperature sensor 5 is controlled in the annealing zones to enable the unit of the heating plate 3 to be stabilized at 60 ℃, in the annealing zones, double-stranded DNA molecules in the first temperature zone are decomposed into single-stranded DNA molecules which are combined with primers, and half-retention replication is carried out under the action of DNA polymerase, so that the number of the DNA molecules flowing along with liquid for one circle is four times that of the original DNA molecules.

The liquid contains DNA molecules, primers, a DNA digesting enzyme and a DNA polymerase, and the activity of the DNA digesting enzyme is activated in the denaturation region, but the activity of the DNA polymerase is not activated; on the other hand, the annealing region activates the activity of DNA polymerase, but does not activate the activity of DNA lyase.

The variable cross-section micro-pipeline 6 can be obtained by photoetching glass sheets, and in order to ensure that the processes of DNA denaturation and polymerization of the outer ring and the inner ring can be completed on time under constant inflow speed, the cross-sectional area of the outer ring micro-pipeline 6 is smaller than that of the inner ring micro-pipeline 6. The advantages of this flow channel are: firstly, the heating plate 3 in the shape of a fan ring can divide the heating plate into a plurality of fan-shaped areas, the time for liquid to flow through different circles of flow passages in a certain fan-shaped area is the same, and based on the flow passage with the special structure, as long as the temperature of the fan-shaped area is controlled, the liquid flows through one circle, DNA molecules can realize the process of cracking → combining → cracking → combining, and more fan-shaped areas can be divided to realize more times of circulation.

The micro-pipeline 6 is spirally arranged for N circles, a micro-channel with a rectangular cross section is carved on one glass sheet by using a photoetching technology and then is bonded with the other glass sheet, the shape of the flow channel is designed, two spiral lines can be taken to determine the track of the flow channel, and the polar coordinate equations are respectively as follows: r is₁＝ar(θ)，

(where r (θ) is a function of θ, and a and b are parameters), a, b, and r are appropriately selected₁The width of the flow channel is as large as 100-200 μm.

The utility model has the advantages that:

firstly, temperature information of the heating plate 3 is transmitted to a multi-path temperature controller by a temperature sensor 5 for temperature stabilization, and then the temperature of the heating plate 3 is controlled to realize the temperature stabilization, so that the trouble of repeated operation of temperature rise and temperature reduction is avoided as long as the preset temperature is stabilized, and only the inflow speed needs to be controlled; and the flow channel with a special structure is adopted, the time for liquid to flow through each section of flow channel in the sector ring area correspondingly divided by the flow channel is equal, in short, "the outer ring is narrow and the inner ring is wide", so that the space size can be reduced, and the pressure drop can be properly reduced.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. A DNA amplification apparatus, characterized in that: the heating rack comprises a rack, be fixed with a plurality of heating plates on the rack, a plurality of heating plates are the annular and arrange, and one side of every heating plate is fixed with temperature sensor, and the upper surface of every heating plate is fixed with the conducting strip respectively, has placed the chip on a plurality of conducting strips, and the inside spiral of chip has arranged many circles of microtubes.

2. The DNA amplification apparatus according to claim 1, wherein: the heat conducting fins are in a fan ring shape.

3. The DNA amplification apparatus according to claim 1, wherein: the cross-sectional area of the outer ring of the micro-pipeline is smaller than that of the inner ring of the micro-pipeline.