CN212955064U

CN212955064U - Embryonic stem cell separator

Info

Publication number: CN212955064U
Application number: CN202020858318.4U
Authority: CN
Inventors: 刘洋; 王飞清; 李艳菊
Original assignee: Affiliated Hospital of Guizhou Medical University; First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine
Current assignee: Affiliated Hospital of Guizhou Medical University; First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine
Priority date: 2020-05-21
Filing date: 2020-05-21
Publication date: 2021-04-13
Anticipated expiration: 2030-05-21

Abstract

The utility model discloses an embryonic stem cell separator, relating to the technical field of separation, which comprises a shell, the side wall of the upper end of the shell is rotationally connected with a cover plate, the side wall of the shell is provided with a fixed groove, the side wall of the cover plate is fixedly connected with a locking mechanism, the side wall of the locking mechanism is propped against the side wall of the fixed groove, a motor groove is arranged on the side wall of the lower end of the shell, a driving motor is fixedly arranged on the side wall of the motor groove, the whole separating mechanism can rotate at high speed by the matching use of the driving motor, the rotating shaft, the clamping plate, the clamping groove, the limiting groove and the limiting block, so as to generate certain centrifugal force, under the effect of centrifugal force, realized the even separation to stem cell among the separating mechanism, avoided adopting motor drive (mixing) shaft and stirring vane to carry out the even separation among the traditional separation mode, and the condition that causes the damage to the cell takes place.

Description

Embryonic stem cell separator

Technical Field

The utility model relates to a separation technical field especially relates to an embryonic stem cell separator.

Background

The embryonic stem cell is a kind of cell separated from early embryo or primitive gonad, it has the characteristics of in vitro culture unlimited proliferation, self-renewal and multidirectional differentiation, the embryonic stem cell can be induced to differentiate into almost all cell types of organism no matter in vitro or in vivo environment, because the number of stem cell is very small, it needs to do non-differentiation proliferation to the stem cell in vitro;

traditional stem cell separator is when using, in order to solve because the weight difference of organizing liquid and the inhomogeneity problem through the filter screen, adopts motor drive (mixing) shaft and stirring vane to stir, and this kind of mechanical stirring causes the damage to the cell easily, influences the quality of separation, and the practicality is relatively poor, for this reason we have proposed an embryonic stem cell separator, solve above problem.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects in the prior art and providing an embryonic stem cell separator.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides an embryonic stem cell separator, includes the casing, the upper end lateral wall of casing rotates and is connected with the apron, the fixed slot has been seted up to the lateral wall of casing, the lateral wall fixed connection latched device of apron, latched device's lateral wall offsets with the lateral wall of fixed slot, the motor groove has been seted up to the lower extreme lateral wall of casing, the lateral wall fixed mounting in motor groove has driving motor, driving motor's the terminal fixedly connected with pivot of output shaft, the lateral wall fixed mounting of pivot has a plurality of screens boards, the inside wall fixed mounting of casing has the buffering circle, the lateral wall sliding connection of buffering circle has separating mechanism, the draw-in groove has been seted up to separating mechanism's lower extreme lateral wall, pivot, screens board all with the lateral wall sliding connection of draw.

Preferably, the locking mechanism comprises a fixing plate fixedly mounted on the side wall of the cover plate, a pulling groove is formed in the side wall of the fixing plate, a convex block is fixedly mounted on the side wall of the fixing plate, and the side wall of the convex block abuts against the side wall of the fixing groove.

Preferably, separating mechanism includes the first bucket that changes of sliding connection on the buffering circle lateral wall, a plurality of spouts and spacing groove have been seted up to the lateral wall of first bucket that changes, the inside wall sliding connection of first bucket that changes has the second to change the bucket, the lateral wall fixed mounting that the bucket was changeed to the second has a plurality of stoppers, the stopper rather than the lateral wall sliding connection of the spacing groove that corresponds, the lateral wall that the second changes the bucket runs through and has seted up a plurality of filter tanks, the equal fixed mounting of filter tank lateral wall has the filter screen.

Preferably, the fixing plate and the bump are both made of elastic plastic materials.

Preferably, the filter screen is made of stainless steel, and the specification of the filter screen is 100-300 meshes.

Preferably, the rotating shaft and the clamping plate are attached to the side wall of the clamping groove.

The utility model has the advantages that: in the utility model, the whole separating mechanism can rotate at high speed by matching the driving motor, the rotating shaft, the clamping plate, the clamping groove, the limiting groove and the limiting block, so that a certain centrifugal force is generated, the stem cells in the separating mechanism are uniformly separated under the action of the centrifugal force, and the condition that the stem cells are damaged due to the uniform separation of the stem cells in the traditional separating mode by adopting the motor-driven stirring shaft and the stirring blades is avoided;

the cover plate, the fixing plate, the convex block and the fixing groove are matched for use, so that the opening and closing of the shell and the fixing function of the cover plate are realized, the condition that liquid is polluted due to the entering of external pollutants during separation is avoided, and the condition that the separation mechanism collides with the inner side wall of the shell in the high-speed rotating process to damage the device can be prevented through the arrangement of the buffer ring;

drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is an enlarged view of a portion a of the present invention.

Fig. 3 is an enlarged view of part B of the present invention.

Fig. 4 is an enlarged view of the portion C of the present invention.

Fig. 5 is a perspective view of a part of the structure of the present invention.

Fig. 6 is a bottom view of the first rotating barrel of the present invention.

Reference numbers in the figures: the device comprises a shell 1, a cover plate 2, a buffer ring 3, a fixing groove 4, a fixing plate 5, a pulling groove 6, a protruding block 7, a motor groove 8, a driving motor 9, a rotating shaft 10, a clamping plate 11, a first rotating barrel 12, a sliding groove 13, a limiting groove 14, a second rotating barrel 15, a limiting block 16, a filter tank 17, a filter screen 18 and a clamping groove 19.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-6, an embryonic stem cell separator comprises a housing 1, a cover plate 2 is rotatably connected to the upper side wall of the housing 1, a fixing groove 4 is formed on the side wall of the housing 1, a locking mechanism is fixedly connected to the side wall of the cover plate 2, the side wall of the locking mechanism abuts against the side wall of the fixing groove 4, the locking mechanism comprises a fixing plate 5 fixedly mounted on the side wall of the cover plate 2, a pulling groove 6 is formed on the side wall of the fixing plate 5, a projection 7 is fixedly mounted on the side wall of the fixing plate 5, the side wall of the projection 7 abuts against the side wall of the fixing groove 4, the fixing plate 5 and the projection, through the matching use among the cover plate 2, the fixing plate 5, the convex block 7 and the fixing groove 4, the opening and closing of the shell 1 and the fixing function of the cover plate 2 are realized, and the condition that external pollutants enter to pollute liquid during separation is avoided;

the side wall of the lower end of the shell 1 is provided with a motor groove 8, the side wall of the motor groove 8 is fixedly provided with a driving motor 9, the tail end of an output shaft of the driving motor 9 is fixedly connected with a rotating shaft 10, the side wall of the rotating shaft 10 is fixedly provided with a plurality of clamping plates 11, the inner side wall of the shell 1 is fixedly provided with a buffer ring 3, the side wall of the buffer ring 3 is connected with a separating mechanism in a sliding manner, and the separating mechanism can be prevented from colliding with the inner side wall of the shell 1 in the high-speed rotating process and damaging the device by the arrangement of the buffer;

the separating mechanism comprises a first rotating barrel 12 connected to the side wall of the buffer ring 3 in a sliding manner, a plurality of sliding grooves 13 and limiting grooves 14 are formed in the side wall of the first rotating barrel 12, a second rotating barrel 15 is connected to the inner side wall of the first rotating barrel 12 in a sliding manner, a plurality of limiting blocks 16 are fixedly mounted on the side wall of the second rotating barrel 15, the limiting blocks 16 are connected with the side wall of the corresponding limiting grooves 14 in a sliding manner, a plurality of filtering grooves 17 are formed in the side wall of the second rotating barrel 15 in a penetrating manner, filtering screens 18 are fixedly mounted on the side walls of the filtering grooves 17, the filtering screens 18 are made of stainless steel and have the specification of 100 meshes and 300 meshes, a clamping groove 19 is formed in the side wall of the lower end of the separating mechanism, the rotating shaft 10 and the clamping plate 11 are connected with the side wall of the clamping groove 19 in a sliding manner, the rotating shaft 10 and the clamping plate 11 are attached to the side wall of the clamping groove, can make whole separating mechanism rotate at a high speed to this produces certain centrifugal force, under the effect of centrifugal force, has realized the homogeneous separation to stem cell among the separating mechanism, has avoided adopting motor drive (mixing) shaft and stirring vane to stir in order to solve among the traditional separation mode because the weight difference of organizing liquid and the inhomogeneous problem through the filter screen, and the condition that causes the damage to the cell takes place.

The working principle is as follows: when the device is used, the fixing plate 5 is pulled outwards by pulling the groove 6, so that the bump 7 is separated from the side wall of the fixing groove 4, the cover plate 2 can be rotated, the liquid to be separated is injected into the second rotating barrel 15 after being opened, the cover plate 2 is closed, the bump 7 is abutted against the side wall of the fixing groove 4 again, and the liquid is prevented from being polluted due to the fact that external pollutants enter during separation;

the driving motor 9 is started to make the rotating shaft 10 start to rotate at high speed, the second rotating barrel 15 is fixed on the inner side wall of the first rotating barrel 12 under the limiting action of the limiting groove 14 and the limiting block 16, because the side wall of the lower end of the first rotating barrel 12 is provided with the clamping groove 19, and the rotating shaft 10 and the clamping plate 11 are attached to the side wall of the clamping groove 19, when the rotating shaft 10 rotates at high speed, the first rotating barrel 12 and the second rotating barrel 15 in the first rotating barrel 12 are driven to rotate at high speed synchronously, under the action of centrifugal force, the liquid to be separated in the second rotating barrel 15 is uniformly thrown to the inner side wall of the second rotating barrel 15, because the side wall of the second rotating barrel 15 is provided with a plurality of filtering grooves 17 in a penetrating way, and the filtering net 18 is fixedly arranged on the side wall of the filtering groove 17, through the filtering of the filtering net 18, the waste liquid can be thrown out of the second rotating barrel 15 and can slide down to the inner bottom of the first rotating barrel 12 through the sliding groove, after the separation is completed, the cover plate 2 can be opened, and the buffer ring 3 is slidably connected with the first rotary barrel 12, and the side wall of the clamping groove 19 is slidably connected with the rotating shaft 10 and the clamping plate 11, so that the first rotary barrel 12 and the second rotary barrel 15 can be taken out from the shell 1 together, and the subsequent operation is convenient.

In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. An embryonic stem cell separator comprises a shell (1) and is characterized in that a cover plate (2) is rotatably connected to the side wall of the upper end of the shell (1), a fixed groove (4) is formed in the side wall of the shell (1), a locking mechanism is fixedly connected to the side wall of the cover plate (2), the side wall of the locking mechanism is abutted to the side wall of the fixed groove (4), a motor groove (8) is formed in the side wall of the lower end of the shell (1), a driving motor (9) is fixedly installed on the side wall of the motor groove (8), a rotating shaft (10) is fixedly connected to the tail end of an output shaft of the driving motor (9), a plurality of clamping plates (11) are fixedly installed on the side wall of the rotating shaft (10), a buffer ring (3) is fixedly installed on the inner side wall of the shell (1), a separating mechanism is slidably connected to the side wall of the buffer ring (3), the rotating shaft (10) and the clamping plate (11) are both connected with the side wall of the clamping groove (19) in a sliding manner.

2. The embryonic stem cell separator as claimed in claim 1, wherein the locking mechanism comprises a fixing plate (5) fixedly mounted on the side wall of the cover plate (2), the side wall of the fixing plate (5) is provided with a pulling groove (6), the side wall of the fixing plate (5) is fixedly provided with a projection (7), and the side wall of the projection (7) is abutted against the side wall of the fixing groove (4).

3. The embryonic stem cell separator according to claim 1, wherein the separating mechanism comprises a first rotating barrel (12) slidably connected to the side wall of the buffer ring (3), the side wall of the first rotating barrel (12) is provided with a plurality of sliding grooves (13) and limiting grooves (14), the inner side wall of the first rotating barrel (12) is slidably connected with a second rotating barrel (15), the side wall of the second rotating barrel (15) is fixedly provided with a plurality of limiting blocks (16), the limiting blocks (16) are slidably connected with the corresponding side wall of the limiting grooves (14), the side wall of the second rotating barrel (15) is provided with a plurality of filtering grooves (17) in a penetrating manner, and the side walls of the filtering grooves (17) are both fixedly provided with filtering nets (18).

4. The embryonic stem cell separator as claimed in claim 2, wherein the fixing plate (5) and the protrusion (7) are made of elastic plastic material.

5. The embryonic stem cell separator as claimed in claim 3, wherein the filter screen (18) is made of stainless steel and has a specification of 100-300 mesh.

6. The embryonic stem cell separator as claimed in claim 1, wherein the shaft (10) and the retaining plate (11) are attached to the side wall of the slot (19).