CN212925053U

CN212925053U - Cell proliferation and toxicity detection kit

Info

Publication number: CN212925053U
Application number: CN202021369011.4U
Authority: CN
Inventors: 常滨滨; 杨泽正
Original assignee: Tianjin Yifan Biotechnology Co ltd
Current assignee: Tianjin Yifan Biotechnology Co ltd
Priority date: 2020-07-13
Filing date: 2020-07-13
Publication date: 2021-04-09
Anticipated expiration: 2030-07-13

Abstract

The utility model provides a cell proliferation and toxicity detect reagent box belongs to cell culture and detects technical field. The cell proliferation and toxicity detection kit comprises a housing mechanism and a box cover mechanism, wherein the housing mechanism comprises a box body component, a culture component, a motor, a first threaded rod, a supporting component and a spring, the box body component comprises a box body, the culture component comprises a culture plate, a sealing cover and a first handle, the supporting component comprises a supporting plate, the motor is connected to the inner wall of the box body, and the first threaded rod is installed on an output shaft of the motor. The utility model discloses a glass lid, cultivation board, culture tank, bull stick, second threaded rod, briquetting, sealed lid, motor, first threaded rod, backup pad and spring's effect to reached the purpose of being convenient for take out reagent, improved current kit and be not convenient for take out reagent, reagent often places in the kit bottom, and people are not convenient for change and abluent problem to reagent.

Description

Cell proliferation and toxicity detection kit

Technical Field

The utility model relates to a cell culture detection area particularly, relates to a cell proliferation and toxicity detect reagent box.

Background

The cell proliferation and toxicity detection kit is a rapid high-sensitivity detection kit which is based on WST-8 and widely applied to cell proliferation and cytotoxicity, the WST-8 is a compound similar to MTT, under the condition that an electronic coupling reagent 1-MethoxyPMS exists, orange yellow water-soluble formazan can be reduced and generated, the more the cell proliferation is, the darker the color is, the more the cytotoxicity is, the lighter the color is, and for the same cells, the shade of the color and the number of the cells are in a linear relationship.

SUMMERY OF THE UTILITY MODEL

In order to make up for the above deficiency, the utility model provides a cell proliferation and toxicity test kit, aims at improving the problem that is not convenient for take out the reagent.

The utility model discloses a realize like this:

the utility model provides a cell proliferation and toxicity detection kit, including shell mechanism and lid mechanism.

The shell mechanism comprises a box body assembly, a culture assembly, a motor, a first threaded rod, a supporting assembly and a spring, the box body assembly comprises a box body, the culture assembly comprises a culture plate, a sealing cover and a first handle, the supporting assembly comprises a supporting plate, the motor is connected with the inner wall of the box body, the first threaded rod is installed on an output shaft of the motor, threads of the first threaded rod penetrate through the supporting plate, two ends of the spring are respectively connected with one side of the supporting plate and one side of the culture plate, a second groove is formed in one side of the culture plate, the sealing cover is arranged in the second groove, the first handle is installed on one side of the sealing cover, a culture groove is formed in one side of the culture plate, the culture groove is arranged at equal intervals, the sealing cover is of a transparent structure, the box cover mechanism comprises a, Briquetting and bull stick, the glass lid rotate connect in box body one side, second threaded rod screw thread run through in the glass lid, the bull stick connect in second threaded rod one end, the briquetting install in the second threaded rod other end.

In an embodiment of the kit for detecting cell proliferation and toxicity of the present invention, the inner surface of the box body is provided with a second threaded hole, and the second threaded hole is in threaded connection with the second threaded rod.

In an embodiment of the kit for detecting cell proliferation and toxicity of the present invention, the inner wall of the box body is fixedly connected with a third sealing ring, and the second threaded rod is threaded through the third sealing ring.

The utility model discloses an in cell proliferation and toxicity detect reagent box embodiment, box body one side fixedly connected with bead, the bead is located box body bottom week side, first recess has been seted up to box body one side, the first sealing washer of first recess inner wall fixedly connected with, the bead with first recess clearance fit, first sealing washer can seal the bead with first groove connection department.

In an embodiment of the kit for detecting cell proliferation and toxicity of the present invention, the inner surface of the supporting plate has a first threaded hole, and the first threaded rod is threaded through the first threaded hole.

In the embodiment of the utility model of a cell proliferation and toxicity test kit, the through-hole has been seted up to the backup pad internal surface, the inside fixedly connected with bracing piece of box body, bracing piece one end fixedly connected with gag lever post, the bracing piece with through-hole clearance fit.

In an embodiment of the kit for detecting cell proliferation and toxicity of the present invention, the inner wall of the second groove is fixedly connected with a second sealing ring.

In an embodiment of the utility model, the housing mechanism further comprises a power supply module, the power supply module comprises a battery box, a storage battery and a charging port, the battery box is connected to the inner wall of the box body, the storage battery is installed on the inner wall of the battery box, the charging port is arranged on one side of the storage battery.

In an embodiment of the kit for detecting cell proliferation and toxicity of the present invention, the charging port extends through the exterior of the case.

In an embodiment of the kit for detecting cell proliferation and toxicity of the present invention, a second handle is fixedly connected to one side of the cover of the glass box.

The utility model has the advantages that: the utility model discloses a cell proliferation and toxicity test kit that obtains through above-mentioned design, when using, open the glass lid through the second handle, put the cell that needs to cultivate into the culture tank on the culture plate of box body inside, put into the culture tank simultaneously with cell toxicity test reagent, close the glass lid, through rotating the bull stick, the bull stick can drive the second threaded rod and rotate, the second threaded rod can drive the briquetting through the second screw hole and move down, the briquetting can make sealed lid compress tightly the culture plate downwards, because glass lid and sealed lid can be transparent, close the glass lid and also can observe the colour of cell toxicity test reagent, the cell proliferation is more fast, the colour is darker, the cell toxicity is bigger, the colour is lighter, can judge toxicity, after having detected, open the glass lid, then take off sealed lid from the culture plate through the first handle, open the motor, the motor can drive first threaded rod and rotate, and first threaded rod can drive the backup pad rebound through first screw hole, and the backup pad can drive culture plate rebound through the spring, makes things convenient for the staff to change and wash to reached and be convenient for take out the purpose of reagent, improved current kit and be not convenient for take out reagent, reagent often is placed in the kit bottom, and people are not convenient for change and abluent problem to reagent.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a schematic structural diagram of a cell proliferation and toxicity detection kit according to an embodiment of the present invention;

fig. 2 is a schematic structural diagram of a housing mechanism according to an embodiment of the present invention;

FIG. 3 is a schematic structural view of a culture assembly according to an embodiment of the present invention;

fig. 4 is a schematic structural diagram of a support assembly according to an embodiment of the present invention;

fig. 5 is a schematic structural diagram of a power supply module according to an embodiment of the present invention;

fig. 6 is a schematic structural view of a box cover mechanism according to an embodiment of the present invention.

In the figure: 10-a housing mechanism; 110-a cartridge body assembly; 111-a cartridge; 112-a first groove; 113-a first seal ring; 120-a culture assembly; 121-growth plate; 122-a second groove; 123-a second sealing ring; 124-culture tank; 125-a sealing cover; 126-a first handle; 130-a motor; 140-a first threaded rod; 150-a support assembly; 151-support plate; 152-a first threaded hole; 153-through holes; 160-a spring; 170-support rods; 180-a limiting rod; 190-power supply components; 191-a battery box; 192-a battery; 193-charging port; 20-a box cover mechanism; 210-glass box cover; 220-ribs; 230-a second handle; 240-a second threaded rod; 250-briquetting; 260-rotating rod; 270-a third seal ring; 280-second threaded hole.

Detailed Description

To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings of the embodiments of the present invention are combined to clearly and completely describe the technical solutions of the embodiments of the present invention, and obviously, the described embodiments are some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

Examples

Referring to fig. 1, the present invention provides a cell proliferation and toxicity detection kit, which includes a housing mechanism 10 and a box cover mechanism 20.

Wherein, lid mechanism 20 one end is rotated and is connected in shell mechanism 10 one end, and shell mechanism 10 is used for cell proliferation and toxicity test, and lid mechanism 20 is used for sealed shell mechanism 10.

Referring to fig. 1, 2, 3 and 4, the housing mechanism 10 includes a box body assembly 110, a culturing assembly 120, a motor 130, a first threaded rod 140, a supporting assembly 150 and a spring 160, the box body assembly 110 includes a box body 111, the culturing assembly 120 includes a culturing plate 121, a sealing cover 125 and a first handle 126, the supporting assembly 150 includes a supporting plate 151, the motor 130 is connected to the inner wall of the box body 111, specifically, the motor 130 is fixedly connected to the inner wall of the box body 111 through a screw, the first threaded rod 140 is mounted on an output shaft of the motor 130, specifically, the first threaded rod 140 is fixedly mounted on an output shaft of the motor 130 through welding, the first threaded rod 140 is threaded through the supporting plate 151, two ends of the spring 160 are respectively connected to one side of the supporting plate 151 and one side of the culturing plate 121, specifically, two ends of the spring 160 are respectively fixedly connected to one side of the supporting plate 151 and, the sealing cover 125 is disposed in the second groove 122, the first handle 126 is disposed on one side of the sealing cover 125, specifically, the first handle 126 is fixedly disposed on one side of the sealing cover 125 by welding, the culture tank 124 is disposed on one side of the culture plate 121, the culture tank 124 is disposed in an equidistant arrangement, the sealing cover 125 is a transparent structure, specifically, the sealing cover 125 is a glass or plastic transparent structure, the culture tank 124 is a place for cell proliferation and toxicity detection, the sealing cover 125 is used for covering the culture tank 124, the motor 130, the first threaded rod 140 and the spring 160 are used for driving the culture plate 121 to move up and down, and the first handle 126 is used for moving the sealing cover.

In some specific embodiments, the inner surface of the box 111 is provided with a second threaded hole 280, the second threaded hole 280 is in threaded connection with a second threaded rod 240, the inner wall of the box 111 is fixedly connected with a third sealing ring 270, specifically, the inner wall of the box 111 is fixedly connected with the third sealing ring 270 by gluing, the second threaded rod 240 is in threaded connection with the third sealing ring 270, the inner surface of the supporting plate 151 is provided with a first threaded hole 152, the first threaded rod 140 is in threaded connection with the first threaded hole 152, the inner surface of the supporting plate 151 is provided with a through hole 153, the inner part of the box 111 is fixedly connected with the supporting rod 170 by welding, one end of the supporting rod 170 is fixedly connected with a limiting rod 180, specifically, one end of the supporting rod 170 is fixedly connected with the limiting rod 180 by welding, the supporting rod 170 is in clearance fit with the through hole 153, the, specifically, the inner wall of the second groove 122 is fixedly connected with a second sealing ring 123 through glue joint, the third sealing ring 270 is used for sealing the joint of the second threaded rod 240 and the glass box cover 210, and the second sealing ring 123 is used for sealing the joint of the sealing cover 125 and the culture plate 121, so that the sealing performance of the kit is improved.

Referring to fig. 1, 2 and 6, the cover mechanism 20 includes a glass cover 210, a second threaded rod 240, a pressing block 250 and a rotating rod 260, the glass cover 210 is rotatably connected to one side of the box body 111, specifically, the glass cover 210 is rotatably connected to one side of the box body 111 through a hinge, the second threaded rod 240 is threaded through the glass cover 210, the rotating rod 260 is connected to one end of the second threaded rod 240, specifically, the rotating rod 260 is fixedly connected to one end of the second threaded rod 240 through welding, the pressing block 250 is mounted to the other end of the second threaded rod 240, specifically, the pressing block 250 is fixedly mounted to the other end of the second threaded rod 240 through welding, one side of the box body 111 is fixedly connected with a rib 220, specifically, one side of the box body 111 is fixedly connected with the rib 220 through welding, the rib 220 is located on the bottom periphery side of the box body 111, one side, specifically, the inner wall of the first groove 112 is fixedly connected with a first sealing ring 113 through glue joint, the rib 220 is in clearance fit with the first groove 112, the joint of the rib 220 and the first groove 112 can be sealed by the first sealing ring 113, one side of the glass box cover 210 is fixedly connected with a second handle 230, specifically, one side of the glass box cover 210 is fixedly connected with a second handle 230 through glue joint, and the second threaded rod 240, the pressing block 250 and the rotating rod 260 are used for enabling the sealing cover 125 to compress the culture plate 121.

Referring to fig. 2 and 5, the housing mechanism 10 further includes a power supply assembly 190, the power supply assembly 190 includes a battery case 191, a storage battery 192 and a charging port 193, the battery case 191 is connected to an inner wall of the case 111, specifically, the battery case 191 is fixedly connected to the inner wall of the case 111 by welding, the storage battery 192 is mounted on the inner wall of the battery case 191, the charging port 193 is disposed on one side of the storage battery 192, the charging port 193 penetrates and extends to the outside of the case 111, and the storage battery 192 and the charging port 193 are used for supplying power to the.

The working principle of the cell proliferation and toxicity detection kit is as follows: when the cell culture box is used, the glass box cover 210 is opened through the second handle 230, cells to be cultured are placed in the culture groove 124 on the culture plate 121 in the box body 111, meanwhile, a cell toxicity detection reagent is placed in the culture groove 124, the glass box cover 210 is closed, the rotating rod 260 is rotated to drive the second threaded rod 240 to rotate, the second threaded rod 240 drives the pressing block 250 to move downwards through the second threaded hole 280, the pressing block 250 downwards seals the cover 125 to press the culture plate 121, the glass box cover 210 and the sealing cover 125 are transparent, the color of the cell toxicity detection reagent can be observed by closing the glass box cover 210, the color is darker when the proliferation of the cells is faster, the cytotoxicity is higher, the color is lighter, the toxicity can be judged, after the detection is finished, the glass box cover 210 is opened, the sealing cover 125 is taken down from the culture plate 121 through the first handle 126, the motor 130 is opened, and the motor 130 drives the first threaded rod 140 to rotate, first threaded rod 140 can drive backup pad 151 rebound through first screw hole 152, and backup pad 151 can drive culture plate 121 rebound through spring 160, makes things convenient for the staff to change and wash to reached the purpose of being convenient for take out reagent, improved current kit and be not convenient for take out reagent, reagent often is placed in the kit bottom, people are not convenient for change and abluent problem to reagent.

It should be noted that the specific model specifications of the motor 130 and the storage battery 192 need to be determined by model selection according to the actual specification of the device, and the specific model selection calculation method adopts the prior art, so detailed description is omitted.

The power supply of the electric motor 130 by the accumulator 192 and its principle will be clear to the skilled person and will not be described in detail here.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A kit for detecting cell proliferation and toxicity is characterized by comprising

The shell mechanism (10), the shell mechanism (10) includes a box body component (110), a culture component (120), a motor (130), a first threaded rod (140), a support component (150) and a spring (160), the box body component (110) includes a box body (111), the culture component (120) includes a culture plate (121), a sealing cover (125) and a first handle (126), the support component (150) includes a support plate (151), the motor (130) is connected to the inner wall of the box body (111), the first threaded rod (140) is installed on the output shaft of the motor (130), the first threaded rod (140) is threaded through the support plate (151), two ends of the spring (160) are respectively connected to one side of the support plate (151) and one side of the culture plate (121), a second groove (122) is formed in one side of the culture plate (121), the sealing cover (125) is arranged in the second groove (122), the first handle (126) is arranged on one side of the sealing cover (125), a culture tank (124) is arranged on one side of the culture plate (121), the culture tanks (124) are arranged at equal intervals, and the sealing cover (125) is of a transparent structure;

lid mechanism (20), lid mechanism (20) include glass lid (210), second threaded rod (240), briquetting (250) and bull stick (260), glass lid (210) rotate connect in box body (111) one side, second threaded rod (240) screw thread run through in glass lid (210), bull stick (260) connect in second threaded rod (240) one end, briquetting (250) install in second threaded rod (240) the other end.

2. The kit for detecting cell proliferation and toxicity of claim 1, wherein the inner surface of the box body (111) is provided with a second threaded hole (280), and the second threaded hole (280) is in threaded connection with the second threaded rod (240).

3. The kit for detecting cell proliferation and toxicity according to claim 1, wherein a third sealing ring (270) is fixedly connected to the inner wall of the box body (111), and the second threaded rod (240) is threaded through the third sealing ring (270).

4. The kit for detecting cell proliferation and toxicity according to claim 1, wherein a rib (220) is fixedly connected to one side of the case (111), the rib (220) is located on the bottom periphery of the case (111), a first groove (112) is formed in one side of the case (111), a first sealing ring (113) is fixedly connected to the inner wall of the first groove (112), the rib (220) is in clearance fit with the first groove (112), and the first sealing ring (113) can seal the joint of the rib (220) and the first groove (112).

5. The kit for detecting cell proliferation and toxicity of claim 1, wherein the supporting plate (151) has a first threaded hole (152) formed on an inner surface thereof, and the first threaded rod (140) is threaded through the first threaded hole (152).

6. The cell proliferation and toxicity detection kit according to claim 1, wherein a through hole (153) is formed in the inner surface of the support plate (151), a support rod (170) is fixedly connected to the inside of the box body (111), a limit rod (180) is fixedly connected to one end of the support rod (170), and the support rod (170) is in clearance fit with the through hole (153).

7. The kit for detecting cell proliferation and toxicity according to claim 1, wherein a second sealing ring (123) is fixedly connected to the inner wall of the second groove (122).

8. The kit for detecting cell proliferation and toxicity according to claim 1, wherein the housing means (10) further comprises a power supply unit (190), the power supply unit (190) comprises a battery case (191), a battery (192) and a charging port (193), the battery case (191) is connected to the inner wall of the case (111), the battery (192) is mounted on the inner wall of the battery case (191), and the charging port (193) is disposed on the side of the battery (192).

9. The cell proliferation and toxicity test kit according to claim 8, wherein the charging port (193) extends through to the exterior of the cassette (111).

10. The kit for detecting cell proliferation and toxicity according to claim 1, wherein a second handle (230) is fixedly connected to one side of the cover glass (210).