CN212925016U - Protease purification device - Google Patents

Abstract

The utility model discloses a protease purification device relates to enzymology technical field. The protease purification device comprises a support frame, a shell fixed on the support frame and a first cylinder fixed on the shell; a centrifugal filtering mechanism for performing centrifugal filtration on the solution containing the protease is arranged in the first cylinder; a centrifugal power mechanism connected with the centrifugal filtering mechanism is arranged in the shell; a fine filtering mechanism is also arranged in the shell; a liquid power mechanism is further arranged in the shell and used for sending liquid centrifugally filtered in the first cylinder into the fine filtering mechanism; the top of first barrel is provided with the top cap, and is equipped with the inside feed liquor pipe of intercommunication first barrel on the top cap, and is equipped with first valve on the feed liquor pipe. This protease purification device can provide good filter effect for protease purification process, and the structure is ingenious, and the filter screen is removable, and the filter screen is changed portably.

Description

Protease purification device

Technical Field

The utility model relates to an enzymology technical field especially relates to a protease purification device.

Background

In the extraction process of the protease, firstly, the biological tissue is crushed, then, clear liquid is taken and added with a medicament, then, the liquid is filled into enzyme extraction equipment, and the liquid with high protease content is obtained by filtering the liquid through the equipment and filtering out impurities. The existing enzyme extraction equipment has poor filtering effect, and after the enzyme extraction equipment is used, impurities are easy to adhere to a filter screen inside the enzyme extraction equipment, and the enzyme extraction equipment is inconvenient to replace.

SUMMERY OF THE UTILITY MODEL

In order to solve the problem of the existence, the utility model provides a protease purification device, this protease purification device can provide good filter effect for protease purification process, and the structure is ingenious, and the filter screen is removable, and the filter screen is changed portably.

In order to achieve the above object, the present invention adopts the following scheme, a protease purification device, comprising a support frame, a housing fixed on the support frame, and a first cylinder fixed on the housing; a centrifugal filtering mechanism for performing centrifugal filtration on the solution containing the protease is arranged in the first cylinder; a centrifugal power mechanism connected with the centrifugal filtering mechanism is arranged in the shell; a fine filtering mechanism is also arranged in the shell; a liquid power mechanism is further arranged in the shell and used for sending liquid centrifugally filtered in the first cylinder into the fine filtering mechanism; the top of first barrel is provided with the top cap, and is equipped with the inside feed liquor pipe of intercommunication first barrel on the top cap, and is equipped with first valve on the feed liquor pipe.

In order to further realize the quick replacement of the filter screen, the centrifugal filter mechanism comprises a round base, a round tray with threads on the outer ring surface and a filter cylinder screwed on the round tray; the round tray is fixed on the round base; the filter cartridge comprises a first circular ring, a plurality of support rods, a second circular ring and an annular first filter screen, wherein the inner ring surface of the first circular ring is provided with threads, two ends of each support rod are respectively arranged on the first circular ring and the second circular ring, the upper edge of the first filter screen is fixed with the second circular ring, and the lower edge of the first filter screen is fixed with the first circular ring; the first circular ring is screwed on the outer circular surface of the circular tray.

Further, the downward vertical projection of the liquid inlet pipe is positioned in the filter cylinder.

Further, the centrifugal power mechanism comprises a motor, a plurality of connecting rods and a bearing; an output shaft of the motor penetrates through a top plate of the shell, the upper end of the connecting rod is fixed with the bottom surface of the round tray, and the lower end of the connecting rod is fixed with the output shaft of the motor; the output shaft of the motor is sleeved with a bearing, and the bearing is installed in a top plate of the shell.

Further, the liquid power mechanism comprises a pump body, a liquid pumping pipe and a liquid sending pipe, wherein a liquid inlet of the pump body is communicated with one end of the liquid pumping pipe, and a liquid outlet of the pump body is communicated with one end of the liquid sending pipe; the other end of the liquid pumping pipe is communicated with the first cylinder; the other end of the liquid delivery pipe is communicated with the fine filtering mechanism.

Furthermore, the fine filtering mechanism comprises a second cylinder with a closed left end and a thread on the outer ring surface of the right end, a third cylinder with a closed right end and a thread on the inner ring surface of the left end, a third ring, a circular second filter screen, a dirt collecting hopper, a blow-down valve, a liquid outlet pipe and a second valve, the other end of the liquid delivery pipe is communicated with the left end of the second cylinder, and the right end of the second cylinder penetrates through the shell; the third circular ring is arranged at the right end of the second cylinder, and the outer diameter of the third circular ring is equal to the inner diameter of the second cylinder; the inner ring surface of a third ring at the edge of the second filter screen is fixed; the left end of the third cylinder body is screwed at the right end of the second cylinder body, the upper end of the sewage collection hopper is communicated with the second cylinder body, the sewage collection hopper is close to the left edge of the third circular ring, and the lower end of the sewage collection hopper is provided with a drain valve; the liquid outlet pipe is communicated with the right end of the third cylinder, and the second valve is arranged on the liquid outlet pipe.

Furthermore, the right-hand end inner ring surface of the second cylinder body is provided with a first annular bulge, the left-hand end inner ring surface of the third cylinder body is provided with a second annular bulge, and the third ring is located between the first annular bulge and the second annular bulge.

Further, the inner diameter of the first annular bulge and the inner diameter of the second annular bulge are both smaller than the outer diameter of the third circular ring.

When the centrifugal filter is used, the motor and the pump body are started, liquid to be filtered is connected into the liquid inlet pipe, the liquid to be filtered flows into the filter cylinder, the rotating filter cylinder centrifugally filters the liquid, impurities are filtered out through the first filter screen and are left in the filter cylinder, the liquid after impurity removal flows into the first cylinder, and then the liquid is sent into the second cylinder through the pump body; the impurities filtered out by the second filter screen can be precipitated in the sewage collecting hopper and can be discharged by opening the drain valve; the liquid after fine filtration by the second filter screen flows into the third cylinder and can be discharged from the liquid outlet pipe.

The utility model has the advantages that: 1) the protease purification device can carry out centrifugal filtration and fine filtration on liquid containing protease, thereby improving the purification degree of the protease; 2) in the centrifugal filtering mechanism, the filter cartridge is in threaded connection with the round tray, when the first filter screen needs to be replaced, the filter cartridge with the first filter screen can be directly detached in a rotating mode, and a new filter cartridge can be mounted in a rotating mode, so that the centrifugal filtering mechanism is convenient and rapid; 3) when the second filter screen needs to be replaced, the second cylinder and the third cylinder are separated in a rotating mode, and a new second filter screen with a third circular ring is directly replaced.

Drawings

FIG. 1 is a schematic sectional view of the protease purification apparatus of the present invention.

Fig. 2 is a schematic structural diagram of the filter cartridge of the present invention.

Fig. 3 is a schematic structural diagram of the fine filtering mechanism of the present invention.

In the figure: 1-a support frame; 2-shell, 21-centrifugal power mechanism, 211-motor, 212-connecting rod, 213-bearing, 22-liquid power mechanism, 221-pump body, 222-liquid suction pipe, 223-liquid sending pipe, 23-fine filtering mechanism, 231-second cylinder, 2311-first annular bulge, 232-third cylinder, 2321-second annular bulge, 233-third ring, 234-second filter screen, 235-dirt collecting hopper, 236-dirt discharging valve, 237-liquid outlet pipe, 238-second valve; 3-first cylinder, 31-centrifugal filter mechanism, 311-round base, 312-round tray, 313-filter cartridge, 3131-first ring, 3132-support bar, 3133-second ring, 3134-first filter screen, 32-top cover, 33-liquid inlet pipe, 331-first valve.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.

It is to be noted that unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the present invention belongs.

In the description of the present application, it is to be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience of description and simplicity of description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting.

Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

In this application, unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can include, for example, fixed connections, removable connections, or integral parts; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In this application, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through intervening media. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

As shown in fig. 1 to 3, a protease purification apparatus includes a support frame 1, a housing 2 fixed to the support frame 1, and a first barrel 3 fixed to the housing 2; a centrifugal filtering mechanism 31 is arranged in the first cylinder 3, and can be used for carrying out centrifugal filtering on the solution containing the protease to filter out certain impurities; a centrifugal power mechanism 21 is arranged in the shell 2 and can provide rotary power for the centrifugal filtering mechanism 31; a fine filtering mechanism 23 is further arranged in the shell 2 and is used for carrying out secondary filtering on the liquid after centrifugal filtering, so that the impurity removal effect is improved; a liquid power mechanism 22 is further arranged in the shell 2, and the liquid power mechanism 22 sends the liquid centrifugally filtered in the first cylinder 3 to a fine filtering mechanism 23; the top of first barrel 3 is provided with top cap 32, and is equipped with the inside feed liquor pipe 33 of intercommunication first barrel 3 on the top cap 32, and is equipped with first valve 331 on the feed liquor pipe 33.

In order to further realize the quick replacement of the filter screen, the centrifugal filter mechanism 31 comprises a round base 311, a round tray 312 with threads on the outer annular surface, and a filter cartridge 313 screwed on the round tray 312; the round tray 312 is fixed on the round base 311; the filter cartridge 313 includes a first ring 3131 with threads on an inner circumferential surface, a plurality of support rods 3132, a second ring 3133, and a first ring 3134, wherein two ends of the support rod 3132 are respectively disposed on the first ring 3131 and the second ring 3133, an upper edge of the first filter mesh 3134 is fixed to the second ring 3133, and a lower edge of the first filter mesh 3134 is fixed to the first ring 3131; the first ring 3131 is screwed on the outer circumferential surface of the circular tray 312. The first ring 3131, the plurality of support rods 3132, and the second ring 3133 form an integral body, and the edge of the first filter mesh 3134 may be adhered to the first ring 3131 and the second ring 3133. The centrifugal filter mechanism 31 rotates, and the liquid to be filtered entering the centrifugal filter mechanism 31 from the liquid inlet pipe 33 rotates under the centrifugal action, so as to achieve the effect of rapid filtration, the impurities are blocked in the filter cartridge 313 by the first filter mesh 3134, and the liquid flows into the first cylinder 3.

Here, the diameter of the circular base 311 is larger than that of the circular tray 312, so that the threaded section of the filter cartridge 313 is prevented from being disengaged from the threaded section of the circular tray 312 during the tightening of the filter cartridge 313. In addition, when the first filter screen 3134 needs to be replaced, the top cover 32 may be directly opened, the filter cartridge 313 may be unscrewed and removed, and finally a new first filter screen 3134 may be replaced, and the filter cartridge 313 may be screwed to the circular tray 312; for ease of replacement of the filter cartridge 313, preparation of a plurality of filter cartridges 313 for replacement may be achieved.

In order to prevent the filter cartridge 313 and the round tray 312 from being separated from each other during rotation, right-handed threads are formed on the round tray 312, and the centrifugal filter mechanism 31 rotates counterclockwise; the circular tray 312 is provided with left-handed threads, and the centrifugal filter mechanism 31 rotates clockwise.

Further, the downward vertical projection of the liquid inlet pipe 33 is located in the filter cartridge 313, so that the liquid flowing out of the liquid inlet pipe 33 can directly enter the filter cartridge 313.

The centrifugal power mechanism 21 provides rotary power for the centrifugal filtering mechanism 31, and the centrifugal power mechanism 21 comprises a motor 211, a plurality of connecting rods 212 and a bearing 213; an output shaft of the motor 211 penetrates through a top plate of the shell 2, the upper end of the connecting rod 212 is fixed with the bottom surface of the round tray 312, and the lower end of the connecting rod 212 is fixed with the output shaft of the motor 211; the output shaft of the motor 211 is sleeved with a bearing 213, and the bearing 213 is installed in the top plate of the housing 2. Here, the bearing 213 is a watertight seal bearing.

In order to accelerate the filtering speed of the fine filtering mechanism 23, the liquid power mechanism 22 includes a pump body 221, a liquid pumping pipe 222 and a liquid sending pipe 223, a liquid inlet of the pump body 221 is communicated with one end of the liquid pumping pipe 222, and a liquid outlet of the pump body 221 is communicated with one end of the liquid sending pipe 223; the other end of the liquid pumping pipe 222 is communicated with the first barrel 3; the other end of the liquid delivery pipe 223 is communicated with the fine filter mechanism 23. The pump body 221 transports the liquid in the first cylinder 3 to the fine filter mechanism 23.

Further, the fine filtering mechanism 23 includes a second cylinder 231 with a closed left end and a threaded outer ring surface at the right end, a third cylinder 232 with a closed right end and a threaded inner ring surface at the left end, a third ring 233, a circular second filter screen 234, a dirt collecting hopper 235, a dirt discharging valve 236, a liquid outlet pipe 237 and a second valve 238, the other end of the liquid feeding pipe 223 is communicated with the left end of the second cylinder 231, and the right end of the second cylinder 231 passes through the housing 2; the third ring 233 is arranged at the right end of the second cylinder 231, and the outer diameter of the third ring 233 is equal to the inner diameter of the second cylinder 231; the inner ring surface of the third ring 233 at the edge of the second filter screen 234 is fixed; the left end of the third cylinder 232 is screwed at the right end of the second cylinder 231, the upper end of the dirt collecting hopper 235 is communicated with the second cylinder 231, the dirt collecting hopper 235 is close to the left edge of the third ring 233, and the lower end of the dirt collecting hopper 235 is provided with a drain valve 236; the liquid outlet pipe 237 is communicated with the right end of the third cylinder 232, and the second valve 238 is arranged on the liquid outlet pipe 237. The liquid is sent into the second cylinder 231 by the pump 221, and due to the pressurization of the pump 221 on the liquid, the second filter screen 234 can rapidly filter the liquid, the filtered liquid can be discharged from the liquid outlet tube 237, and the impurities can be internally blocked at the left side of the second filter screen 234 and deposited in the dirt collecting hopper 235, and the impurities in the dirt collecting hopper 235 can be discharged by opening the dirt discharge valve 236.

When the second filter 234 needs to be replaced, the third cylinder 232 is simply unscrewed from the second cylinder 231, and the second filter 234 and the third ring 233 can be taken out for replacement.

In order to ensure that the third ring 233 does not move in the second cylinder 231, a first annular protrusion 2311 is arranged on the inner ring surface of the right end of the second cylinder 231, a second annular protrusion 2321 is arranged on the inner ring surface of the left end of the third cylinder 232, and the third ring 233 is located between the first annular protrusion 2311 and the second annular protrusion 2321. The inner diameter of the first annular protrusion 2311 and the inner diameter of the second annular protrusion 2321 are both smaller than the outer diameter of the third ring 233.

When the device is used, the motor 211 and the pump body 221 are started, liquid to be filtered is connected into the liquid inlet pipe 33, the liquid to be filtered flows into the filter cylinder 313, the rotating filter cylinder 313 centrifugally filters the liquid, impurities are filtered out through the first filter screen 3134 and are left in the filter cylinder 313, the liquid after impurity removal flows into the first cylinder 3 and then is sent into the second cylinder 231 through the pump body 221; the impurities filtered out through the second screen 234 may be settled in the dirt collection hopper 235 and may be discharged by opening the drain valve 236; the liquid fine-filtered by the second filter 234 flows into the third cylinder 232 and can be discharged from the liquid outlet tube 237.

Claims (8)

1. A protease purification device, its characterized in that: the device comprises a support frame (1), a shell (2) fixed on the support frame (1) and a first cylinder (3) fixed on the shell (2); a centrifugal filtering mechanism (31) for carrying out centrifugal filtration on the solution containing the protease is arranged in the first cylinder body (3); a centrifugal power mechanism (21) connected with a centrifugal filtering mechanism (31) is arranged in the shell (2); a fine filtering mechanism (23) is also arranged in the shell (2); a liquid power mechanism (22) is further arranged in the shell (2), and the liquid power mechanism (22) sends the liquid centrifugally filtered in the first cylinder (3) to a fine filtering mechanism (23); the top of first barrel (3) is provided with top cap (32), and is equipped with the inside feed liquor pipe (33) of first barrel (3) of intercommunication on top cap (32), and is equipped with first valve (331) on feed liquor pipe (33).

2. The protease purification apparatus according to claim 1, wherein: the centrifugal filtering mechanism (31) comprises a round base (311), a round tray (312) with threads on the outer ring surface and a filter cartridge (313) screwed on the round tray (312); the round tray (312) is fixed on the round base (311); the filter cartridge (313) comprises a first circular ring (3131) with threads on the inner annular surface, a plurality of support rods (3132), a second circular ring (3133) and a first annular filter screen (3134), wherein two ends of each support rod (3132) are respectively arranged on the first circular ring (3131) and the second circular ring (3133), the upper edge of the first filter screen (3134) is fixed with the second circular ring (3133), and the lower edge of the first filter screen (3134) is fixed with the first circular ring (3131); the first ring (3131) is screwed on the outer ring surface of the circular tray (312).

3. The protease purification apparatus according to claim 2, wherein: the downward vertical projection of the liquid inlet pipe (33) is positioned in the filter cartridge (313).

4. The protease purification apparatus according to claim 2, wherein: the centrifugal power mechanism (21) comprises a motor (211), a plurality of connecting rods (212) and a bearing (213); an output shaft of the motor (211) penetrates through a top plate of the shell (2), the upper end of the connecting rod (212) is fixed with the bottom surface of the round tray (312), and the lower end of the connecting rod (212) is fixed with the output shaft of the motor (211); the output shaft of the motor (211) is sleeved with a bearing (213), and the bearing (213) is installed in the top plate of the shell (2).

5. The protease purification apparatus according to claim 1, wherein: the liquid power mechanism (22) comprises a pump body (221), a liquid extracting pipe (222) and a liquid sending pipe (223), wherein a liquid inlet of the pump body (221) is communicated with one end of the liquid extracting pipe (222), and a liquid outlet of the pump body (221) is communicated with one end of the liquid sending pipe (223); the other end of the liquid extracting pipe (222) is communicated with the first cylinder (3); the other end of the liquid sending pipe (223) is communicated with the fine filtering mechanism (23).

6. The protease purification apparatus according to claim 5, wherein: the fine filtering mechanism (23) comprises a second cylinder (231) with a closed left end and a thread on the outer ring surface of the right end, a third cylinder (232) with a closed right end and a thread on the inner ring surface of the left end, a third ring (233), a circular second filter screen (234), a dirt collecting hopper (235), a drain valve (236), a liquid outlet pipe (237) and a second valve (238), the other end of the liquid conveying pipe (223) is communicated with the left end of the second cylinder (231), and the right end of the second cylinder (231) penetrates through the shell (2); the third circular ring (233) is arranged at the right end of the second cylinder (231), and the outer diameter of the third circular ring (233) is equal to the inner diameter of the second cylinder (231); the inner ring surface of a third ring (233) at the edge of the second filter screen (234) is fixed; the left end of the third cylinder (232) is screwed at the right end of the second cylinder (231), the upper end of the dirt collecting hopper (235) is communicated with the second cylinder (231), the dirt collecting hopper (235) is close to the left edge of the third circular ring (233), and the lower end of the dirt collecting hopper (235) is provided with a drain valve (236); the liquid outlet pipe (237) is communicated with the right end of the third cylinder (232), and the second valve (238) is arranged on the liquid outlet pipe (237).

7. The protease purification apparatus according to claim 6, wherein: the inner ring surface of the right end of the second cylinder (231) is provided with a first annular bulge (2311), the inner ring surface of the left end of the third cylinder (232) is provided with a second annular bulge (2321), and the third ring (233) is located between the first annular bulge (2311) and the second annular bulge (2321).

8. The protease purification apparatus according to claim 7, wherein: the inner diameter of the first annular bulge (2311) and the inner diameter of the second annular bulge (2321) are both smaller than the outer diameter of the third circular ring (233).

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