CN212877338U

CN212877338U - Freeze and deposit pipe

Info

Publication number: CN212877338U
Application number: CN202021864466.3U
Authority: CN
Inventors: 高爽; 靳新; 王娟; 刘帅; 常德华
Original assignee: BOE Technology Group Co Ltd; BOE Regenerative Medicine Technology Co Ltd
Current assignee: BOE Technology Group Co Ltd; BOE Regenerative Medicine Technology Co Ltd
Priority date: 2020-08-31
Filing date: 2020-08-31
Publication date: 2021-04-06
Anticipated expiration: 2030-08-31

Abstract

The utility model provides a cryopreserved pipe specifically can include: a pipe body, a pipe cap and a floating piece; wherein, an accommodating cavity is arranged in the tube body; the pipe cap is connected with the pipe body in a matching way; the float piece cup joints outside the body, and can follow the body slides to switch between primary importance and second place primary importance, float piece's the side of going up with the pipe cap is connected secondary importance, float piece's downside with the bottom parallel and level of body. The embodiment of the utility model provides an in, through the piece that floats not only can avoid water to get into the body and cause the pollution, has reduced the operating time that the cell was come back to life or consciousness, can also improve freeze and deposit the stability of managing to place on the operation panel, prevent freeze and deposit the phenomenon that the pipe appears empting.

Description

Freeze and deposit pipe

Technical Field

The utility model relates to a cell freezes deposits equipment technical field, especially relates to a freezes deposits pipe.

Background

The cell cryopreservation process needs to use a cryopreservation tube to contain cell suspension, and the cryopreservation tube is usually formed by connecting a tube cap and a tube body through threads. When the cell is recovered, the freezing tube needs to be soaked in warm water so as to melt the cell suspension from a solid state to a liquid state.

In the prior art, when cells are recovered, the cryopreservation tube is clamped frequently, or other floating tools are used to enable the tube cap of the cryopreservation tube to be higher than the water surface, so that water is prevented from entering the tube body to cause pollution. So operate not only to increase operating time to can't avoid water to contact the junction of body and pipe cap completely. Moreover, when the cell suspension is filled into the freezing tube or the cell suspension in the freezing tube is taken out, the phenomenon that the freezing tube is inclined is easy to occur because the contact area between the bottom of the freezing tube and the operating platform is small.

SUMMERY OF THE UTILITY MODEL

In view of the above, the present invention has been developed to provide a cryopreservation tube that overcomes or at least partially solves the above problems.

In order to solve the problem, the utility model discloses a cryopreserved pipe, include: a pipe body, a pipe cap and a floating piece; wherein the content of the first and second substances,

an accommodating cavity is formed in the tube body;

the pipe cap is connected with the pipe body in a matching way;

the float piece cup joints outside the body, and can follow the body slides to switch between primary importance and second place primary importance, float piece's the side of going up with the pipe cap is connected secondary importance, float piece's downside with the bottom parallel and level of body.

Optionally, the outer diameters of the pipe bodies increase in sequence from the top surface to the bottom surface of the pipe body;

float and be provided with first through-hole on the piece, first through-hole cup joints outside the body, follow the side to the direction of downside of going up of floating the piece, the internal diameter of first through-hole increases progressively in proper order, first through-hole is in the internal diameter of downside is less than the external diameter of the bottom surface of body.

Optionally, the tube body is a cylinder, and a protrusion is arranged on the outer wall of the tube body close to the bottom;

the utility model discloses a pipe body, including the pipe body, the pipe body is provided with the downside, float and be provided with the second through-hole on the piece, the second through-hole cup joints outside the pipe body, the inner wall of second through-hole is close to the position of downside is provided with the recess, the shape of recess with bellied shape matches float the piece slide extremely under the condition of second position, the recess with protruding joint.

Optionally, the cryopreservation tube further comprises: the weight piece, the weight piece set up in the bottom of body.

Optionally, a gasket is disposed in the cap on a side close to the accommodating cavity, and the gasket is made of at least one of polyurethane, halogenated butyl rubber, polyisoprene and silicone rubber.

Optionally, the pipe cap and the pipe body are in threaded connection, or the pipe cap and the pipe body are in clamping connection.

Optionally, the inner diameter of the lower end of the pipe cap is equal to the outer diameter of the upper end of the pipe body, or the inner diameter of the lower end of the pipe cap is equal to the inner diameter of the upper end of the pipe body.

Optionally, the material of the floating member is selected from: at least one of polyurethane foam, polystyrene foam, polyethylene foam and polyvinyl chloride foam.

Optionally, the floating member is a hollow plastic member or a glass member.

Optionally, the cryopreservation tube further comprises: a stopper located between the tube body and the cap, the stopper having an outer diameter greater than an outer diameter of the tube body;

in the first position, the upper side of the float is in abutment with the stop member.

The utility model discloses a following advantage:

the embodiment of the utility model provides an in, the cryopreserving pipe can include the float, the float cup joints outside the body, and can follow the body slides to switch between primary importance and second place. Need use when freezing and depositing the pipe and carry out cell recovery, can with float and slide extremely the primary importance, the side of going up of float can with be connected with the pipe cap float under the buoyancy of float, the body can float in aqueous perpendicularly, the pipe cap can be located the surface of water, avoids water to get into the body and causes the pollution, has reduced operating time. When need toward freezing intraductal filling cell suspension of depositing or will freeze the intraductal cell suspension of depositing and take out, can with float slide extremely the second position, float the downside with the bottom parallel and level of body, float the downside can with the bottom of body contacts in the operation panel together, the increase freeze the area of contact of depositing pipe and operation panel, improve freeze the stability of depositing the pipe and placing on the operation panel, prevent freeze the phenomenon that the pipe appears empting.

Drawings

FIG. 1 is a schematic structural view of a freezing pipe in a first position according to the present invention;

FIG. 2 is a schematic structural view of the freezer pipe of FIG. 1 in a second position;

FIG. 3 is a schematic top view of the freezing pipe shown in FIG. 1;

FIG. 4 is a schematic structural view of another cryopreservation tube of the present invention;

FIG. 5 is a schematic structural view of another cryopreservation tube of the present invention;

description of reference numerals: 10-tube body, 101-containing cavity, 102-bulge, 11-tube cap, 111-external thread structure, 12-floating piece, 13-blocking piece and 14-counterweight piece.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description.

The embodiment of the utility model provides a cryopreserving pipe, cryopreserving pipe can be used for preserving or shifting the cell suspension. Specifically, the cell suspension can be placed in the freezing tube for freezing preservation, when cell recovery is needed, the freezing tube can be soaked in warm water to melt the cell suspension from a solid state to a liquid state, and after cell recovery, the freezing tube can be placed on an operation table to take out the recovered cells. In practical application, when cell resuscitation is performed, attention needs to be paid to avoid water from entering the freezing pipe through the joint of the pipe cap and the pipe body, and when cell suspension is filled into the freezing pipe or the cell suspension in the freezing pipe is taken out, the freezing pipe needs to be prevented from toppling.

Referring to fig. 1, a schematic structural diagram of a freezer pipe in a first position according to the present invention is shown, referring to fig. 2, a schematic structural diagram of a freezer pipe in a second position shown in fig. 1 is shown, referring to fig. 3, a schematic structural diagram of a top view of a freezer pipe shown in fig. 1 is shown, the freezer pipe may specifically include: a pipe body 10, a pipe cap 11 and a floating member 12; wherein, an accommodating cavity 101 is arranged in the tube body 10, and the accommodating cavity 101 can be used for accommodating cell suspension; the pipe cap 11 is connected with the pipe body 10 in a matching way; the float 12 is sleeved outside the tubular body 10 and is slidable along the tubular body 10 to switch between a first position shown in fig. 1, in which the upper side of the float 12 is connected to the cap 11, and a second position shown in fig. 2, in which the lower side of the float 12 is flush with the bottom of the tubular body 10.

Specifically, when cell resuscitation needs to be performed by using the cryopreservation tube, the floating member 12 can be slid to the first position, in the first position, the upper side surface of the floating member 12 can be connected with the tube cap 11, the tube body 10 can vertically float in water under the buoyancy action of the floating member 12, the tube cap 11 can be located on the water surface, the water is prevented from entering the tube body 10 to cause pollution, and the operation time is shortened. When need add cell suspension or take out the cell suspension in the cryopreserving pipe toward cryopreserving intraductal, can slide showy piece 12 extremely the second position to the downside that makes showy piece 12 and the bottom parallel and level of body 10, the downside that showy piece 12 can contact in the operation panel with the bottom of body 10 together, the increase cryopreserving pipe and the area of contact of operation panel, improve cryopreserving pipe places the stability on the operation panel, prevents the phenomenon that the cryopreserving pipe appears empting.

For example, the pipe cap 11 and the pipe body 10 may be screwed, or the pipe cap 11 and the pipe body 10 may be clamped, or the pipe cap 11 may be plugged into the pipe body 10 in a plug manner, so as to achieve the connection between the pipe cap 11 and the pipe body 10. The embodiment of the utility model provides a can not do the restriction to the connected mode between pipe cap 11 and body 10.

As shown in fig. 2, in order to facilitate the threaded connection of the cap 11 and the pipe body 10, an external thread structure 111 may be provided on the cap 11, and an internal thread structure may be correspondingly provided on the pipe body 10, and the internal thread structure may be engaged with the external thread structure 111 to achieve the threaded connection between the cap 11 and the pipe body 10.

Alternatively, the cap 11 and the tube 10 may be connected by an outward rotation connection, that is, the inner diameter of the lower end of the cap 11 may be equal to the outer diameter of the upper end of the tube 10, or the cap 11 and the tube 10 may be connected by an inward rotation connection, that is, the inner diameter of the lower end of the cap 11 is equal to the inner diameter of the upper end of the tube 10. The utility model discloses do not limit to this.

The embodiment of the utility model provides an in, be close to the one side that holds chamber 101 in the pipe cap 11 and can be provided with packing ring (not shown in the figure) to leakproofness when improving pipe cap 11 and body 10 and being connected avoids the phenomenon of weeping appears in the cryopreserving pipe. Specifically, the material of the gasket may be at least one selected from polyurethane, halogenated butyl rubber, polyisoprene, and silicone rubber, and the embodiment of the present invention is not particularly limited to the material of the gasket.

For example, the material of the tube body 10 and the tube cap 11 may be selected from: at least one of polypropylene, polyethylene, polycarbonate and polystyrene. The material of the tube body 10 and the material of the tube cap 11 may be the same or different.

In order to make the shrinkage of the tube body 10 and the tube cap 11 consistent and avoid a gap between the tube body 10 and the tube cap 11 in the cryogenic environment, the tube body 10 and the tube cap 11 should be made of the same material as much as possible.

Specifically, the shape of the accommodating cavity 101 in the tube 10 may be conical or cylindrical, and the embodiment of the present invention may not be limited to the specific shape of the accommodating cavity 101. The bottom surface of the receiving cavity 101 may be a sharp bottom or a round bottom. In order to reduce local ice crystal nucleation growth at the time of cooling down due to the non-smooth edge, which may damage cells in the receiving chamber 101, the bottom surface of the receiving chamber 101 should preferably be rounded.

In practical application, the volume of the accommodating cavity 101 in the tube body 10 is the actual volume of the cryopreservation tube, and in order to avoid the increase of the volume of the cell suspension in the accommodating cavity 101 after freezing and solidification and damage to the cryopreservation tube, the nominal volume of the cryopreservation tube should be less than or equal to 0.8 times of the actual volume of the accommodating cavity 101.

For example, the nominal volume of the cryopreservation tube may be 0.1ml to 10ml, for example, 0.5ml, 1ml, 1.5ml, 2ml, 3ml, 5ml, 10ml, and the like, and the embodiments of the present invention are not particularly limited to the nominal volume of the cryopreservation tube.

In an optional embodiment of the present invention, the cryopreservation tube may further include: a stopper 13, the stopper 13 being located between the pipe body 10 and the pipe cap 11, an outer diameter of the stopper 13 being larger than an outer diameter of the pipe body 10; in said first position, the upper side of the float 12 abuts against the stop 13 to prevent the float 12 from falling out of the upper end of the tubular body 10.

In practical application, the blocking member 13 may be a blocking sheet independent from the pipe body 10 and the pipe cap 11, and may also be a sheet structure fixed on the pipe body 10 or the pipe cap 11, and the embodiment of the present invention may not be limited to the specific form of the blocking member 13.

As shown in fig. 1 and 2, the pipe 10 may have a circular truncated cone shape. That is, the outer diameters of the pipe bodies 10 are sequentially increased from the top surface to the bottom surface of the pipe body 10; be provided with first through-hole 121 on the float 12, first through-hole 121 cup joints outside body 10, and from the side of going up to the direction of downside of float 12, the internal diameter of first through-hole 12 increases progressively in proper order to first through-hole 12 slope that make can with the slope adaptation of body 10 outer wall, be convenient for float 12 and slide from top to bottom along body 10. The first through-hole 121 has an inner diameter at the lower side of the floating member 12 smaller than an outer diameter of the bottom surface of the tubular body 10 to prevent the floating member 12 from being separated from the bottom of the tubular body 10.

In practical application, first through-hole 121 is less than the external diameter of body 10 bottom surface at the internal diameter of float 12 downside, specifically can do, and first through-hole 121 slightly is lighter than the external diameter of body 10 bottom surface at the internal diameter of float 12 downside, and like this, float 12 can pass through the frictional force card in the bottom of body 10, float 12 downside can with the bottom parallel and level of body 10, float 12 downside can contact in the operation panel with the bottom of body 10 together, increase freeze the area of contact of depositing pipe and operation panel, improve freeze the stability of depositing the pipe and placing on the operation panel, prevent freeze the phenomenon that the pipe appears empting.

Referring to fig. 4, which is a schematic structural view illustrating another cryopreservation tube of the present invention, as shown in fig. 4, the tube body 10 is a cylinder, and a protrusion 102 is disposed on an outer wall of the tube body 10 near the bottom; be provided with the second through-hole on the float 12, the second through-hole cup joints outside body 10, the position that the inner wall of second through-hole is close to float 12 downside is provided with the recess, the shape of recess matches with protruding 102's shape, slides extremely at float 12 under the condition of second position, recess and protruding 102 joint to avoid float 12 to break away from the bottom of body 10.

It should be noted that, in practical applications, the blocking member 13 disposed on the top of the tube 10 may also adopt a form similar to the form of the groove and the protrusion 102 in fig. 4, so as to prevent the floating member 12 from coming off from the top of the tube 10, which is not limited by the embodiment of the present invention.

Referring to fig. 5, a schematic structural diagram of another cryopreservation tube of the present invention is shown, as shown in fig. 5, the cryopreservation tube may further include: and the counterweight 14 is arranged at the bottom of the pipe body, and the counterweight 14 is arranged at the bottom of the pipe body. The weight member 14 may be embodied as a metal block or the like embedded in the bottom of the pipe body 10 in advance, and the weight member 14 may be used to weight the pipe body 10.

Specifically, in the case of cell resuscitation using the cryopreservation tube, in order that the cryopreservation tube filled with the frozen cell suspension can be vertically suspended in water, the center of gravity of the cryopreservation tube should be located below the height of 1/2. Through set up balancing weight 14 in body 10 bottom, can reduce the focus of whole freezing pipe of depositing, so that it can suspend in aqueous perpendicularly to freeze to deposit the pipe, avoids water to pass through the entering freeze and deposit intraductal to cause the pollution to the cell suspension.

In an alternative embodiment of the present invention, the material of the floating member 12 may be selected from: at least one of polyurethane foam, polystyrene foam, polyethylene foam and polyvinyl chloride foam. In practice, the floating member 12 made of the above material may have a large buoyancy because of the advantage of the low density of the above material.

In an alternative embodiment of the present invention, the floating member 12 may be a hollow plastic or glass member. In practical applications, the floating member 12 is designed to be hollow, so that the overall density of the floating member 12 can be reduced, and the floating member 121 has a larger buoyancy.

In practice, one skilled in the art may choose to use a low density material for the float 12, or may prepare the float 12 as a hollow structure, or a combination thereof. The embodiment of the utility model provides a do not limit to this.

Although the floating member 12 is not limited to a specific material or a specific structure, the floating member 12 and the tube 10 should generate a buoyancy force greater than the weight of the cryopreservation tube after the cryopreservation tube is filled with the cell suspension, that is, the weight of water corresponding to the volume of the floating member 12 and the tube 10 should be greater than the weight of the cryopreservation tube after the cryopreservation tube is filled with the cell suspension. Thus, cell recovery using the cryopreservation tube is performed only when the cryopreservation tube is filled with a cell suspension and then can be vertically suspended in water.

To sum up, the embodiment of the present invention provides a freezing pipe at least can include following advantage:

Finally, it should also be noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or terminal that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or terminal. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other like elements in a process, method, article, or terminal that comprises the element.

The cryopreservation tube provided by the utility model is introduced in detail, and the principle and the implementation mode of the utility model are explained by applying a specific example, and the explanation of the above embodiment is only used for helping to understand the method and the core idea of the utility model; meanwhile, for the general technical personnel in the field, according to the idea of the present invention, there are changes in the specific implementation and application scope, to sum up, the content of the present specification should not be understood as the limitation of the present invention.

Claims

1. A cryopreservation tube, comprising: a pipe body, a pipe cap and a floating piece; wherein the content of the first and second substances,

an accommodating cavity is formed in the tube body;

the pipe cap is connected with the pipe body in a matching way;

2. The cryopreservation tube of claim 1, wherein the outer diameters of the tube bodies increase in sequence from the top surface to the bottom surface of the tube bodies;

3. The cryopreservation tube of claim 1, wherein the tube body is a cylinder, and a protrusion is arranged on the outer wall of the tube body near the bottom;

4. The cryopreservation tube of claim 1, further comprising: the weight piece, the weight piece set up in the bottom of body.

5. The cryopreservation tube of claim 1, wherein a gasket is arranged at one side of the tube cap close to the containing cavity, and the gasket is made of at least one of polyurethane, halogenated butyl rubber, polyisoprene and silicon rubber.

6. The cryopreservation tube of claim 1, wherein the tube cap and the tube body are in threaded connection, or in clamping connection.

7. The cryopreservation tube of claim 1, wherein the inner diameter of the lower end of the cap is equal to the outer diameter of the upper end of the tube body, or the inner diameter of the lower end of the cap is equal to the inner diameter of the upper end of the tube body.

8. The cryopreservation tube of claim 1, wherein the floating member is made of a material selected from the group consisting of: at least one of polyurethane foam, polystyrene foam, polyethylene foam and polyvinyl chloride foam.

9. The cryopreservation tube of claim 1, wherein the floating member is a hollow plastic or glass member.

10. The cryopreservation tube of any one of claims 1 to 9, further comprising: a stopper located between the tube body and the cap, the stopper having an outer diameter greater than an outer diameter of the tube body;