CN212770732U - Liquid transfer device for cell culture solution - Google Patents
Liquid transfer device for cell culture solution Download PDFInfo
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- CN212770732U CN212770732U CN202020511042.2U CN202020511042U CN212770732U CN 212770732 U CN212770732 U CN 212770732U CN 202020511042 U CN202020511042 U CN 202020511042U CN 212770732 U CN212770732 U CN 212770732U
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- pipetting
- cell culture
- hose
- storage container
- pipette
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Abstract
The utility model discloses a cell culture liquid is with moving liquid device, include: a first storage container for storing a cell culture solution; a second storage container for receiving a cell culture solution; the pipetting hose comprises 2 interface ends, wherein one interface end is communicated with the first storage container, and the other interface end is opened towards the opening of the second storage container; a pipetting drive element for driving the cell culture fluid in the pipetting hose to flow inside the pipetting hose by applying pressure to the side wall of the pipetting hose; and the control handle is in communication connection with the pipetting driving element and is used for sending a signal to the pipetting driving element so as to control the pipetting driving element to be switched on and off. Through the utility model provides an among the prior art cell culture liquid big, the high problem of pollution risk of application of sample error that exists when moving the liquid.
Description
Technical Field
The utility model belongs to biological cultivation equipment field, concretely relates to cell and microbial cultivation in-process's cell culture liquid shifts liquid-transfering device for operation.
Background
In the cell culture process, the conventional pipetting method mainly adopts an electric suction aid combined with a long pipetting tube with scales, the pipetting tube is a glass piece, and the liquid suction and liquid discharge switch of the suction aid is controlled by fingers to perform pipetting operation. The method is characterized in that before liquid removal, one end of a pipette is inserted into an aspirator to be connected with the aspirator, the pipette is inserted into a culture medium container containing cell culture liquid, a liquid suction button of the aspirator is controlled by a finger to suck the liquid into the pipette, then the pipette is moved to a target position, the liquid discharge button of the aspirator is pressed by the finger to discharge the liquid in the pipette, and liquid transfer is realized. The pipette is inserted into the suction aid, so that the problem of loose insertion and matching with the suction aid can be caused, when the pipette is used for pipetting, cell culture solution can be leaked, liquid drops can splash on a table top, potential pollution hidden dangers can be caused, the pipette is long in length and inserted into the pipette, the hand is easy to shake during operation, sundries are easy to collide, and the pollution risk is high;
the cell culture liquid volume that shifts out from the culture medium when needs are big, when need the partial shipment to a plurality of different storage containers, then need insert the culture medium in at the pipette many times and get the liquid, the pipette can frequent business turn over culture medium container, especially under the cell culture scene of great scale, and work load is very big, and is very inconvenient, easy fatigue, easily makes mistakes to the volume of cell culture liquid mainly relies on naked eye visual measurement scale, and the application of sample error is big.
SUMMERY OF THE UTILITY MODEL
The utility model discloses to the problem that the application of sample error that cell culture liquid exists when moving liquid is big, the pollution risk is high among the prior art, provide a move liquid device for cell culture liquid is used, can reduce the infection risk, reduce the application of sample error.
In order to realize the purpose of the utility model, the utility model adopts the following technical scheme to realize:
a pipette device for cell culture solution for transferring cell culture solution from a first storage container to a second storage container, the pipette device comprising:
the pipetting hose comprises 2 interface ends, wherein one interface end is communicated with the first storage container, and the other interface end is opened towards the opening of the second storage container;
a pipetting drive element for driving the cell culture fluid in the pipetting hose to flow inside the pipetting hose by applying pressure to the side wall of the pipetting hose;
and the control handle is in communication connection with the pipetting driving element and is used for sending a signal to the pipetting driving element so as to control the pipetting driving element to be switched on and off.
Furthermore, the end part of the liquid transfer hose is provided with a detachable pipe joint communicated with the liquid transfer hose.
Further, an interface end at the second storage container end is located above the second storage container.
Furthermore, the device also comprises a bracket, and the pipetting hose is detachably arranged on the bracket.
Further, the liquid transfer hose and the pipe joint are disposable consumables.
Furthermore, the control handle comprises a main controller, and the main controller is in communication connection with the pipetting driving element.
Furthermore, a wireless communication module which can be communicated with each other is arranged between the control handle and the pipetting drive element.
Further, the pipetting drive element is a peristaltic pump.
Furthermore, the control handle also comprises a handle.
Compared with the prior art, the utility model discloses an advantage is with positive effect:
the utility model provides a liquid-transfering device for cell culture liquid, when using, accessible control handle sends control signal to move liquid drive arrangement, and control moves liquid drive arrangement and moves, exerts pressure to moving liquid hose for cell culture liquid moves in moving liquid hose, in transferring the cell culture liquid ration in the first storage container to the second storage container, because move liquid drive arrangement for through the pressure effect move liquid hose realize cell culture liquid transfer, consequently, move liquid hose and move liquid no connection between the liquid drive arrangement, can not produce the problem that cell culture liquid reveals in the liquid hose, reduced the infection risk;
when a large amount of cell culture solution is extracted, the liquid transfer driving device can be controlled to continuously work to extract the solution, if a plurality of second storage containers are required to place the cell culture solution, only the interface end of the liquid transfer hose needs to be moved into another second storage container from one second storage container, the culture medium containers do not need to be frequently accessed, time and labor are saved, mistakes are not easy to make, and the sample adding precision is improved;
the volume of getting of adding of cell culture liquid mainly realizes through the control handle control pipetting drive element switch, need not to watch the scale through human eye and realizes, has improved the precision of application of sample.
Other features and advantages of the present invention will become more apparent from the following detailed description of the invention when read in conjunction with the accompanying drawings.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
Fig. 1 is a schematic structural view of an embodiment of a pipette device for a cell culture solution according to the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings and embodiments.
It should be noted that in the description of the present invention, the terms of direction or positional relationship indicated by the terms "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, which are merely for convenience of description, and do not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The present invention provides an embodiment of a liquid-transfering device for cell culture liquid, which is used for transferring cell culture liquid from a first storage container 100 to a second storage container 200, as shown in fig. 1. Specifically, the first storage container 100 is used for storing a cell culture solution, and the first storage container 100 may be a cell culture medium container in which the cell culture solution is stored.
The number of the second storage containers 200 is 1 or more, and when multiple collections of the cell culture fluid in the first storage container 100 are needed, multiple second storage containers 200 are needed, and no specific limitation is made herein.
A pipetting hose 300 comprising 2 interface ends 310, one interface end 310 being in communication with the first storage container 100, and the other interface end 310 being open to the opening of the second storage container 200;
a pipetting driving element 400 for driving the cell culture fluid in the pipetting hose 300 to flow inside the pipetting hose 300 by applying pressure to the side wall of the pipetting hose 300; preferably, the pipetting drive element 400 is a peristaltic pump.
The pipetting hose 300 is preferably made of silicone, has certain flexibility, can be compressed and not damaged when being acted by the pipetting driving element 400, and can automatically return to the initial state after the pressure on the pipetting hose 300 is eliminated so as to ensure that the pipetting hose 300 can normally work.
A control handle 500, communicatively connected to the pipetting driving element 400, for sending a signal to the pipetting driving element 400 to control the pipetting driving element 400 to open and close.
Further, a detachable pipe joint 600 communicating with the pipette hose 300 is provided at the end of the pipette hose 300.
The function of the pipe joint 600 is: the liquid outlet end of the liquid transfer hose 300 is protected from contacting other objects, other pollution is prevented, and if the pipe joint 600 accidentally touches other objects, the pipe joint 600 can be replaced without replacing the whole liquid transfer hose 300.
Further, an interface end 310 at the end of the second storage container 200 is located above the second storage container 200. Compared with the conventional pipette, the pipette hose 300 has the mouth end 310 with a downward opening, so that liquid can be effectively prevented from being splashed to the outside.
Further, a support 700 is included, and the pipetting hose 300 is detachably arranged on the support 700. The pipette hose 300 can be fixed to the holder 700 after installation when the operator performs other operations, so that the pipette hose 300 can be temporarily stored and taken out at any time, and the risk of contamination is reduced. The specific pipetting hose 300 can be clamped on the support 700 through a buckle, or a hook is arranged on the support 700, and the pipetting hose 300 can be directly hung on the support 700, so that the situation that the pipe joint 600 at the end of the pipetting hose 300 contacts other articles to cause pollution is avoided.
Preferably, the liquid-transferring hose 300 and the pipe joint 600 in this embodiment are disposable consumables, and both are sterilized and pyrogen-removed when used, so that the liquid-transferring hose 300 and the pipe joint 600 can be thrown away directly after the cell culture solution in one of the cell culture medium containers is extracted, and can be disposed as disposable consumables, thereby avoiding the cross infection due to repeated use.
In order to realize the communication with the pipetting driving element 400, a main controller is further arranged in the control handle 500 in the embodiment, the main controller is in communication connection with the pipetting driving element 400, and in order to facilitate the communication between the control handle 500 and the pipetting driving element 400, a wireless communication module capable of communicating with each other is further arranged between the control handle 500 and the pipetting driving element 400. The wireless communication module may be a bluetooth communication module or a WIFI wireless communication module, and is not limited herein.
Of course, the master controller and the pipetting driving element 400 in this embodiment can be directly connected through a wired communication line to realize the communication between the master controller and the pipetting driving element.
In specific control, the pipetting driving element 400 can be controlled to be turned on or off by a main controller positioned in the control handle 500, so that quantitative pipetting effect is realized.
For convenience of operation, the control handle 500 in this embodiment further includes a grip 510 convenient for a user to hold, so that the operator can hold the control handle 500 and the interface end 310 of the liquid-transferring hose 300 to be discharged with one hand to perform operation, thereby facilitating operation and use.
Specifically, when performing a pipetting operation, one of the mouthpiece ends 310 of the pipetting hose 300 is inserted into the first storage container 100, and then the control handle 500 is held by hand. At the same time, the interface end 310 on the liquid outlet side of the pipette hose 300 is held so as to be open to the opening of the second storage container 200, and when the interface end is set, a control program for controlling the pipetting amount of the pipetting driving element 400 is provided in the pipetting driving element 400, the pipetting driving element 400 is controlled to be turned on by the control handle 500, and the interface end is turned on when the pipetting driving device receives a signal from the control handle 500, and starts operation to transfer the cell culture liquid in the first storage container 100 to the second storage container 200.
The pipetting driving element 400 enables the movement of the cell culture fluid in the pipetting hose 300 by applying pressure to the pipetting hose 300, and the pipetting hose 300 and the pipetting driving element 400 do not need to be connected and do not need to be in contact with the cell culture fluid, so that the problem of leakage of the cell culture fluid is avoided, and the risk of infection is reduced.
Control through control handle 500 and move liquid drive element 400 and open and close to the control moves the control that the volume of the cell culture liquid that flows through and then realized the quantitative transfer of fine bubble culture liquid in the drive element 400, need not to obtain the transfer volume of cell culture liquid through the scale, need not artifical observation, has improved the precision of moving the liquid.
In addition, a plurality of second storage containers 200 may be correspondingly provided in this embodiment and arranged in sequence, and when the cell culture solution is transferred, the cell culture solution may be transferred from the first storage container 100 to one of the second storage containers 200, and when a desired amount is reached, the mouthpiece 310 with the adapter 600 is put into another second storage container 200, and the cell culture solution is continuously circulated in sequence, and the cell culture solution is supplied to all the second storage containers 200.
When the cell culture solution of the second storage containers 200 is transferred, the transfer can be realized only by one pipette, and the pipette does not need to frequently enter and exit the first storage container 100 when the solution is pumped, so that the operation is more convenient and labor-saving, the labor intensity is reduced, and the solution transferring efficiency is improved.
If the pipe joint 600 in the pipette is infected when moving to contact other articles in the plurality of second storage containers 200, the pipe joint 600 can be directly detached for replacement, and the whole pipette does not need to be detached, so that the use is convenient.
The above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or that equivalents may be substituted for elements thereof; such modifications and substitutions do not depart from the spirit and scope of the present invention, which is claimed.
Claims (9)
1. A pipette device for a cell culture solution, the pipette device being used for transferring a cell culture solution from a first storage container to a second storage container, characterized by comprising:
the pipetting hose comprises 2 interface ends, wherein one interface end is communicated with the first storage container, and the other interface end is opened towards the opening of the second storage container;
a pipetting drive element for driving the cell culture fluid in the pipetting hose to flow inside the pipetting hose by applying pressure to the side wall of the pipetting hose;
and the control handle is in communication connection with the pipetting driving element and is used for sending a signal to the pipetting driving element so as to control the pipetting driving element to be switched on and off.
2. A pipette device for a cell culture solution according to claim 1, wherein a detachable tube adapter communicating with the pipette hose is provided at an end of the pipette hose.
3. A pipette device for cell culture liquid according to claim 1, wherein the mouthpiece on the side of the second storage container is located above the second storage container.
4. A pipette device for a cell culture solution according to claim 1, further comprising a holder, and the pipette hose is detachably attached to the holder.
5. A pipetting device for cell culture liquid according to claim 2 characterized in that the pipetting hose and the tube adapter are disposable consumables.
6. A pipetting device as recited in claim 1 wherein the control handle includes a master controller that is communicatively connected to the pipetting drive element.
7. A pipetting device for a cell culture solution according to claim 6 characterized in that a wireless communication module capable of communicating with each other is further provided between the control handle and the pipetting drive member.
8. A pipetting device for cell culture liquids according to claim 1, characterized in that the pipetting drive element is a peristaltic pump.
9. A pipetting device as recited in claim 6 wherein the control handle further comprises a grip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020511042.2U CN212770732U (en) | 2020-04-09 | 2020-04-09 | Liquid transfer device for cell culture solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020511042.2U CN212770732U (en) | 2020-04-09 | 2020-04-09 | Liquid transfer device for cell culture solution |
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| Publication Number | Publication Date |
|---|---|
| CN212770732U true CN212770732U (en) | 2021-03-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202020511042.2U Active CN212770732U (en) | 2020-04-09 | 2020-04-09 | Liquid transfer device for cell culture solution |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023098791A1 (en) * | 2021-12-01 | 2023-06-08 | 南京金斯瑞生物科技有限公司 | Control system for cell therapy and control method therefor |
-
2020
- 2020-04-09 CN CN202020511042.2U patent/CN212770732U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023098791A1 (en) * | 2021-12-01 | 2023-06-08 | 南京金斯瑞生物科技有限公司 | Control system for cell therapy and control method therefor |
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Inventor after: Yan Chengxia Inventor after: Zhou Feng Inventor after: Liu Rundong Inventor after: Cheng Lihui Inventor after: Teng Peikun Inventor after: Zhang Lu Inventor after: Wu Songjie Inventor after: Liu Yu Inventor after: Qin Wenqiang Inventor after: Liu Ying Inventor after: Guo Fanfan Inventor before: Zhang Lu Inventor before: Wu Songjie Inventor before: Teng Peikun Inventor before: Liu Rundong |