CN212741399U

CN212741399U - Cell culture dish

Info

Publication number: CN212741399U
Application number: CN202020913628.1U
Authority: CN
Inventors: 仲志婷; 唐隽
Original assignee: 901th Hospital of the Joint Logistics Support Force of Pla
Current assignee: 901th Hospital of the Joint Logistics Support Force of Pla
Priority date: 2020-05-25
Filing date: 2020-05-25
Publication date: 2021-03-19
Anticipated expiration: 2030-05-25

Abstract

The utility model belongs to the technical field of the culture dish, a cell culture dish is provided, including the external ware body, the internal ware body and ware lid, still include: first sealing plug, a plurality of reset spring, first permanent magnet, knob, pivot, second permanent magnet and cell filter equipment, the utility model provides a cell culture dish is convenient for change the culture medium.

Description

Cell culture dish

Technical Field

The utility model relates to a culture dish technical field, concretely relates to cell culture dish.

Background

A petri dish is a laboratory vessel used for the cultivation of microorganisms or cells, generally consisting of a bottom and a lid of a disc, mainly made of plastic and glass.

However, the conventional cell culture dish is inconvenient for replacing the culture medium, and the dish cover needs to be opened every time of replacement, so that the inside of the culture dish is easily polluted, and the culture of cells is influenced.

SUMMERY OF THE UTILITY MODEL

To the defect among the prior art, the utility model aims at providing a cell culture dish to change the culture medium.

In order to achieve the above object, the utility model provides a cell culture dish, including the external ware body, the internal ware body and ware lid, still include: a first sealing plug, a plurality of reset springs, a first permanent magnet, a knob, a rotating shaft, a second permanent magnet and a cell filtering device,

the inner peripheral surface of the outer vessel body is step-shaped, the inner vessel body is arranged in the outer vessel body and keeps sealed with the outer vessel body, the vessel cover is arranged at the top of the inner vessel body and keeps sealed with the inner vessel body,

a first channel for adding culture medium is arranged in the middle of the inner dish body, the top of the first channel extends out of the dish cover and keeps sealed with the dish cover, a first sealing cover is arranged at the top of the first channel, a sub-channel is arranged on the side wall of the first channel and is communicated with the inner dish body and the first channel,

the bottom of the inner ware body is provided with a first groove, the axial lead of the first groove is superposed with the axial lead of the first channel, the inner diameter of the first groove is larger than the outer diameter of the first channel, the first sealing plug is arranged at the first groove and keeps sealing with the first groove,

the inner ware body is uniformly provided with a plurality of mounting holes around the axial lead of the first channel, a plurality of reset springs are in one-to-one correspondence with the mounting holes and are respectively arranged in the mounting holes, and both ends of the reset springs are respectively fixedly connected with the inner ware body and the first sealing plug,

the first permanent magnet is embedded at the bottom of the first sealing plug,

a second groove is arranged on the periphery of the external utensil body, the knob is rotatably arranged in the second groove,

the rotating shaft is arranged in the outer vessel body and is rotationally connected with the outer vessel body, one end of the rotating shaft is fixedly connected with the knob,

the middle part of the rotating shaft is provided with a mounting frame which is positioned below the first permanent magnet, the second permanent magnet is arranged in the mounting frame,

the bottom of the inner vessel body is provided with a first through hole which is positioned between the inner diameter of the first groove and the outer diameter of the first channel and is communicated with the inner vessel body and the outer vessel body,

the cell filtering device is arranged at the first through hole.

Further, the bottom of the first channel is communicated with the external utensil body,

the sub-channel is arranged in the middle of the first channel,

the first sealing plug is step-shaped, one end with the smaller diameter extends upwards into the first channel, is positioned below the sub-channel and keeps sealed with the first channel, and the other end with the larger diameter is positioned in the first groove and keeps sealed with the first groove.

Furthermore, the number of the sub-channels is multiple, and the multiple sub-channels are uniformly distributed around the axis line of the first channel.

Furthermore, the number of the first through holes is multiple, and the multiple first through holes are uniformly distributed around the axis line of the first channel.

Further, the first sealing cover comprises a cover body, a bacterium filtering device and a second sealing plug, the cover body is provided with a hollow inner cavity, the inner diameter of the inner cavity is matched with the outer diameter of the first channel, the bacterium filtering device is arranged in the middle of the inner cavity, and the second sealing plug is arranged at the top of the cover body.

Further, still include: and the bottom of the external utensil body is provided with a second through hole, the second through hole is communicated with the external utensil body and the outside, and the third sealing plug is arranged at the second through hole and keeps sealing with the second through hole.

The utility model has the advantages that:

the utility model provides a cell culture dish when changing the internal culture medium of interior ware, rotates 180 with the knob, and the knob drives the pivot and rotates 180, and the pivot drives the second permanent magnet and rotates 180, and at this moment the opposite one side of magnetism of second permanent magnet and first permanent magnet is relative, according to the principle that opposite poles attract, and the suction of first permanent magnet and second permanent magnet is greater than reset spring energetically. Under the action of the attraction force of the first permanent magnet and the second permanent magnet, the first sealing plug moves downwards to open the first through hole. Under the action of the cell filtering device, the cells cultured in the inner dish body are left in the inner dish body, and the culture medium flows into the outer dish body through the first channel.

When the culture medium in the inner dish body is drained, the knob is rotated for 180 degrees again, and the knob drives the second permanent magnet to rotate for 180 degrees again. At this time, the second permanent magnet is opposite to the side with the same magnetism of the first permanent magnet, and a repulsive force is generated between the first permanent magnet and the second permanent magnet according to the principle that like poles repel each other. Under the action of the tension of the return spring and the magnetic force of the first permanent magnet and the second permanent magnet. The first sealing plug rises to seal the first through hole.

Then, the first sealing cover is opened, the culture medium is added into the first channel, and the culture medium flows into the sub-channel along the first channel and then flows into the inner dish body through the sub-channel. And after the culture medium is added, covering the first sealing cover. In the process, the dish cover is not opened, and the outer dish body is not opened, so that the pollution to the inner dish body and the outer dish body is reduced. And when the culture medium is replaced, the culture medium in the inner dish body is directly replaced, so that the waste of the culture medium is avoided.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the technical solutions in the prior art will be briefly described below. Throughout the drawings, like elements or portions are generally identified by like reference numerals. In the drawings, elements or portions are not necessarily drawn to scale.

FIG. 1 is a perspective view of a cell culture dish according to an embodiment of the present invention;

FIG. 2 is a front view of the cell culture dish of FIG. 1;

FIG. 3 is a cross-sectional view taken along line A-A of FIG. 2;

FIG. 4 is an enlarged view at B shown in FIG. 3;

FIG. 5 is an enlarged view at C shown in FIG. 3;

FIG. 6 is a perspective view of the rotating shaft of the cell culture dish shown in FIG. 1.

Reference numerals:

100-outer vessel body, 110-second groove, 120-second through hole, 200-inner vessel body, 210-first channel, 220-first sealing cover, 221-cover body, 222-bacteria filtering device, 223-second sealing plug, 224-inner cavity, 230-sub channel, 240-first groove, 250-mounting hole, 260-first through hole, 300-vessel cover, 400-first sealing plug, 410-first permanent magnet, 420-reset spring, 500-knob, 600-rotating shaft, 610-mounting frame, 700-second permanent magnet, 800-cell filtering device and 900-third sealing plug.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.

It is to be noted that unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the present invention belongs.

In the description of the present application, it is to be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience of description and simplicity of description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting.

Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

In this application, unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can include, for example, fixed connections, removable connections, or integral parts; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In this application, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through intervening media. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

As shown in fig. 1-6, the utility model provides a cell culture dish, which comprises an outer dish body 100, an inner dish body 200 and a dish cover 300.

The cell culture dish further comprises: a first sealing plug 400, a first permanent magnet 410, a plurality of return springs 420, a knob 500, a rotating shaft 600, a second permanent magnet 700, and a cell filtering device 800.

The inner peripheral surface of the vessel body 100 is stepped, the inner vessel body 200 is mounted in the vessel body 100 and sealed with the vessel body 100, and the vessel lid 300 is mounted on the top of the inner vessel body 200 and sealed with the inner vessel body 200.

The middle part of the inner dish body 200 is provided with a first channel 210 for adding culture medium, and the top of the first channel 210 extends out of the dish cover 300 and keeps sealed with the dish cover 300. The first sealing cover 220 is arranged at the top of the first channel 210, and preferably, the bottom of the first sealing cover 220 is abutted against the dish cover 300, so that the combining force of the dish cover 300 and the inner dish body 200 can be increased. The side wall of the first channel 210 is provided with a sub-channel 230, the sub-channel 230 is communicated with the inner vessel body 200 and the first channel 210, and preferably, the sub-channel 230 is inclined downwards, so that when a culture medium is added into the inner vessel body 200, the culture medium is not or less remained in the first channel 210.

The bottom of the inner vessel body 200 is provided with a first groove 240, the axial lead of the first groove 240 coincides with the axial lead of the first channel 210, the inner diameter of the first groove 240 is larger than the outer diameter of the first channel 210, and the first sealing plug 400 is installed at the first groove 240 and keeps sealing with the first groove 240.

The inner vessel body 200 is uniformly provided with a plurality of mounting holes 250 around the axial lead of the first channel 210, the plurality of reset springs 420 are in one-to-one correspondence with the plurality of mounting holes 250 and are respectively mounted in the plurality of mounting holes 250, and two ends of the reset springs 420 are respectively fixedly connected with the inner vessel body 200 and the first sealing plug 400. Naturally, the return spring 420 is under tension to keep the first sealing plug 400 seated in the first recess 240 and sealed with the first recess 240.

The first permanent magnet 410 is embedded in the bottom of the first sealing plug 400.

The periphery of the external utensil body 100 is provided with a second groove 110, the knob 500 is rotatably arranged in the second groove 110,

the rotating shaft 600 is installed in the vessel body 100 and is rotatably connected with the vessel body 100, and one end of the rotating shaft 600 is fixedly connected with the knob 500.

The middle part of the rotating shaft 600 is provided with a mounting frame 610, the mounting frame 610 is positioned below the first permanent magnet 410, and the second permanent magnet 700 is fixedly mounted in the mounting frame 610. In a natural state, the same magnetic sides of the first permanent magnet 410 and the second permanent magnet 700 are opposite, and according to the principle that like poles repel each other, a repulsive force is generated between the first permanent magnet 410 and the second permanent magnet 700, and the first sealing plug 400 cannot descend.

The bottom of the inner vessel body 200 is provided with a first through hole 260, the first through hole 260 is used for placing a culture medium in the inner vessel body 200, the first through hole 260 is located between the inner diameter of the first groove 240 and the outer diameter of the first channel 210, and the first through hole 260 is communicated with the inner vessel body 200 and the outer vessel body 100.

The cell filtering device 800 is installed at the first through hole 260 and is used for filtering the cells in the inner vessel body 200 and preventing the cells from flowing out of the first through hole 260 along with the culture medium.

In one embodiment, the bottom of the first channel 210 is in communication with the capsule body 100.

The sub-channel 230 is disposed at the middle of the first channel 210.

The first sealing plug 400 is stepped with the smaller diameter end extending up into the first passage 210 and below the sub-passage 230 and remaining sealed to the first passage 210. This facilitates the guiding of the first sealing stopper 400, and no or little medium remains in the first channel 210 when medium is added to the vessel body 200. The larger diameter end of which is located within the first groove 240 and remains sealed to the first groove 240.

In one embodiment, the number of sub-channels 230 is multiple, and the multiple sub-channels 230 are evenly distributed around the axis of the first channel 210. This accelerates the flow of the culture medium in the first channel 210 into the vessel body 200.

In one embodiment, the number of the first through holes 260 is plural, and the plural first through holes 260 are uniformly distributed around the axial center line of the first channel 210. This accelerates the flow of the culture medium in the inner vessel body 200 into the outer vessel body 100.

In one embodiment, the first sealing cover 220 includes a cover 221, a bacteria filter 222 and a second sealing plug 223, the cover 221 defines a hollow inner cavity 224, an inner diameter of the inner cavity is adapted to an outer diameter of the first channel 210, and specifically, both ends of the cover 221 are connected to the outside. The bacteria filter 222 is fixedly installed in the middle of the inner cavity 224 for filtering out contaminants such as bacteria in the air. A second sealing plug 223 is fixedly mounted on the top of the cover 221 for sealing the cover 221.

When the cultivation needs to be carried out, the second sealing plug 223 is taken down, air enters the first passage 210 through the inner cavity 224, pollutants such as bacteria in the air are filtered out under the action of the bacteria filtering device 222, and clean air passes through the first passage 210 and enters the inner vessel body 200 through the sub-passage 230. When anaerobic culture is performed, the second sealing plug 223 is closed, so that the sealing of the inner vessel body 200 can be maintained. This structure helps to perform aerobic and anaerobic culture in the inner vessel body 200, thereby improving the adaptability of the culture dish, and simultaneously reducing the pollution to the inner vessel body 200 under the action of the bacteria filtering device 222.

In one embodiment, further comprising: and a third sealing plug 900, wherein the bottom of the external vessel body 100 is provided with a second through hole 120, the second through hole 120 is communicated with the external vessel body 100 and the outside, and the third sealing plug 900 is arranged at the second through hole 120 and keeps sealed with the second through hole 120. This facilitates the removal of the culture medium from the vessel body 100 without removing the inner culture medium, thereby reducing contamination of the inner culture medium.

The working principle of the utility model is as follows:

when the culture medium container is used, when a culture medium in the inner container body 200 needs to be replaced, the knob 500 is rotated 180 degrees, the knob 500 drives the rotating shaft 600 to rotate 180 degrees, the rotating shaft 600 drives the second permanent magnet 700 to rotate 180 degrees, at the moment, the opposite sides of the second permanent magnet 700 and the first permanent magnet 410 are opposite, and according to the principle that opposite poles attract each other, the attraction force of the first permanent magnet 410 and the second permanent magnet 700 is larger than the large force of the reset spring 420. Under the suction force of the first and second

permanent magnets

410 and 700, the first sealing plug 400 moves downward, opening the first through hole 260. Under the action of the cell filtration device 800, the cells cultured in the inner vessel body 200 are retained in the inner vessel body 200, and the culture medium flows into the outer vessel body 100 through the first channel 210.

When the culture medium in the inner vessel body 200 is exhausted, the knob 500 is rotated 180 degrees again, and the knob 500 drives the second permanent magnet 700 to rotate 180 degrees again. At this time, the second permanent magnet 700 is opposite to the same side of the first permanent magnet 410, and a repulsive force is generated between the first permanent magnet 410 and the second permanent magnet 700 according to the principle that like poles repel each other. Under the tensile force of the return spring 420 and the magnetic force of the first permanent magnet 410 and the second permanent magnet 700. The first sealing plug 400 rises to seal the first through hole 260.

Then, the first sealing cover 220 is opened, the culture medium is added into the first channel 210, flows into the sub-channel 230 along the first channel 210, and then flows into the inner vessel body 200 through the sub-channel 230. After the culture medium is added, the first sealing cover 220 is covered. In this process, the capsule 300 is not opened, and the capsule 100 is not opened, thereby reducing contamination of the inner capsule 200 and the outer capsule 100. In addition, when the culture medium is replaced, the culture medium in the inner dish body 200 is directly replaced, so that the waste of the culture medium is avoided.

After the culture medium in the inner dish body 200 is replaced, the third sealing plug 900 can be directly opened, so that the culture medium in the outer dish body 100 flows out from the second through hole 120, and the third sealing plug 900 can be opened after the culture medium in the outer dish body 100 is accumulated to a certain extent.

In the specification of the present invention, a large number of specific details are explained. It is understood, however, that embodiments of the invention may be practiced without these specific details. In some instances, well-known methods, structures and techniques have not been shown in detail in order not to obscure an understanding of this description.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not substantially depart from the scope of the embodiments of the present invention, and are intended to be covered by the claims and the specification.

Claims

1. The utility model provides a cell culture dish, includes the external ware body, the internal ware body and ware lid, its characterized in that: further comprising: a first sealing plug, a plurality of reset springs, a first permanent magnet, a knob, a rotating shaft, a second permanent magnet and a cell filtering device,

the first permanent magnet is embedded at the bottom of the first sealing plug,

the cell filtering device is arranged at the first through hole.

2. A cell culture dish according to claim 1, wherein:

the bottom of the first channel is communicated with the external utensil body,

the sub-channel is arranged in the middle of the first channel,

3. A cell culture dish according to claim 2, wherein: the number of the sub-channels is multiple, and the multiple sub-channels are uniformly distributed around the axial lead of the first channel.

4. A cell culture dish according to claim 3, wherein: the number of the first through holes is multiple, and the first through holes are uniformly distributed around the axial lead of the first channel.

5. A cell culture dish according to claim 4, wherein: the first sealing cover comprises a cover body, a bacterium filtering device and a second sealing plug, the cover body is provided with a hollow inner cavity, the inner diameter of the inner cavity is matched with the outer diameter of the first channel, the bacterium filtering device is arranged in the middle of the inner cavity, and the second sealing plug is arranged at the top of the cover body.

6. A cell culture dish according to any of claims 1-5, wherein: further comprising: and the bottom of the external utensil body is provided with a second through hole, the second through hole is communicated with the external utensil body and the outside, and the third sealing plug is arranged at the second through hole and keeps sealing with the second through hole.