CN212741313U - Kit for second-generation sequencing nucleic acid extraction - Google Patents
Kit for second-generation sequencing nucleic acid extraction Download PDFInfo
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- CN212741313U CN212741313U CN202021451759.9U CN202021451759U CN212741313U CN 212741313 U CN212741313 U CN 212741313U CN 202021451759 U CN202021451759 U CN 202021451759U CN 212741313 U CN212741313 U CN 212741313U
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- box body
- adhesive film
- setting adhesive
- barrel
- nucleic acid
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Abstract
The utility model discloses a reagent box for second-generation sequencing nucleic acid extraction, which comprises a box body with a base, wherein the upper surface of the box body is provided with a plurality of reagent grooves which are arranged at intervals and extend downwards to form a recess; the left and right sides of box body is equipped with multiplexing closing cap device, and multiplexing closing cap device is including setting up the section of thick bamboo and the rolling section of thick bamboo that unreel in the box body upper end left and right sides, puts and is left right side parallel arrangement between section of thick bamboo and the rolling section of thick bamboo, the utility model discloses a non-setting adhesive film that this department was pressed down makes its upper surface of pasting at the box body, like this alright carry out the shutoff to the reagent groove, puncture non-setting adhesive film alright realize taking a sample through the syringe needle of pipettor during the sample, after the sample, owing to be in sterile environment, consequently rotate the rolling section of thick bamboo and carry out the rolling switching to the non-setting adhesive film of punctureing, press down non-setting adhesive film again afterwards and make its upper surface of pasting at the box body, thereby.
Description
Technical Field
The utility model relates to a biological processing field especially relates to a kit that is used for two take place ofs sequencing nucleic acid to draw.
Background
Most of the existing reagent kits for extracting the second-generation sequencing nucleic acid are of disposable structures, and can not be used up in a single time, but the existing reagent kits are of single-time unsealing structures and have no secondary sealing structures on the whole, so that the waste situation is easy to occur in the using process.
SUMMERY OF THE UTILITY MODEL
The utility model discloses an it is not enough to overcome above-mentioned condition, aims at providing the technical scheme that can solve above-mentioned problem.
A reagent kit for second-generation sequencing nucleic acid extraction comprises a kit body with a base, wherein the upper surface of the kit body is provided with a plurality of reagent grooves which are arranged at intervals and extend downwards to form a recess, the reagent grooves are used for storing reagents, and the reagents are quantitatively extracted and used through a liquid-transferring gun when in use;
the reusable cover sealing device comprises an unwinding barrel and a winding barrel which are arranged on the left side and the right side of the upper end of the box body, the unwinding barrel and the winding barrel are arranged in parallel left and right between the unwinding barrel and the winding barrel, the unwinding barrel and the winding barrel both comprise hollow barrels and rotating shafts, the rotating shafts are arranged on the inner side of the barrels and are arranged at intervals with the barrels, bearings are sleeved at the front end and the rear end of each rotating shaft, the outer rings of the bearings are fixedly clamped with the barrels, linearly extending notches are formed in the side surfaces of the barrels, and the notches are located at positions above the upper surface of the box body; the non-setting adhesive film with the non-setting adhesive coated on the lower surface is wound on the rotating shaft in the winding drum, the free edge of the non-setting adhesive film penetrates out of the notch of the winding drum and penetrates into the notch of the winding drum and is wound on the rotating shaft in the winding drum, the winding of the non-setting adhesive film can be realized by rotating the rotating shaft of the winding drum, and the non-setting adhesive film positioned between the winding drum and the winding drum is positioned right above the box body; make it paste the upper surface at the box body through the non-setting adhesive film of this department of pushing down, alright carry out the shutoff to the reagent groove like this, puncture the non-setting adhesive film alright realize taking a sample through the syringe needle of pipettor during the sample, after the sample, owing to be in sterile environment, consequently rotate the winding cylinder and carry out the rolling switching to the non-setting adhesive film who punctures, push down the non-setting adhesive film once more afterwards and make its paste the upper surface at the box body, thereby realize sealed, whole can be with comparatively convenient realization sealing process, thereby realize the multiplexing of reagent.
As a further aspect of the present invention: the side position is installed through the hinge is articulated at the upper surface rear side of box body and is lapped, and integrated into one piece has the hasp on the front edge of apron, and the side position is equipped with the discount that cooperatees the setting with the hasp before the upper surface of box body, utilizes flipping of apron to push down and realizes that the closing cap pushes down the laminating to the non-setting adhesive film simultaneously.
As a further aspect of the present invention: and a hexagonal linkage jack is processed at one end of the rotating shaft of the winding drum and is used for connecting an external rotating device, so that the rotating shaft is driven to rotate for winding.
As a further aspect of the present invention: the non-setting adhesive film is made of a PP material with a single-side coated with glue.
The utility model has the advantages that: the utility model discloses a non-setting adhesive film that pushes down this department makes its upper surface of pasting at the box body, like this alright carry out the shutoff to the reagent groove, puncture non-setting adhesive film alright realize the sample through the syringe needle of pipettor during the sample, after the sample, owing to be in sterile environment, consequently rotate a rolling section of thick bamboo and carry out the rolling to the non-setting adhesive film who punctures and switch, push down non-setting adhesive film once more afterwards and make its upper surface of pasting at the box body, thereby the realization is sealed, wholly can be with comparatively convenient realization sealing process, thereby realize the multiplexing of reagent.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive exercise.
FIG. 1 is a top view of the present invention;
fig. 2 is a schematic front view of the partial structure of the present invention.
In the figure: 1-box body, 2-base, 3-reagent groove, 4-cover plate, 5-hasp, 6-hasp head, 7-reel, 8-reel, 9-self-adhesive film, 10-rotary shaft and 11-linkage jack.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-2, in an embodiment of the present invention, a kit for second generation sequencing nucleic acid extraction includes a box body 1 with a base 2, a plurality of reagent grooves 3 are disposed on an upper surface of the box body 1 at intervals and extend downwards, the reagent grooves 3 are used for storing reagents, and when in use, the reagents are quantitatively extracted and used through a pipette;
the reusable cover sealing device is arranged on the left side and the right side of the box body 1 and comprises an unwinding cylinder 7 and a winding cylinder 8 which are arranged on the left side and the right side of the upper end of the box body 1, the unwinding cylinder 7 and the winding cylinder 8 are arranged in parallel left and right, the unwinding cylinder 7 and the winding cylinder 8 both comprise hollow cylinders and rotating shafts 10, the rotating shafts 10 are arranged on the inner sides of the cylinders and are arranged at intervals with the cylinders, bearings are sleeved at the front end and the rear end of each rotating shaft 10, the outer rings of the bearings are fixedly clamped with the cylinders, linearly extending notches are formed in the side surfaces of the cylinders, and the notches are positioned above the upper surface of the box body 1; a self-adhesive film 9 with the lower surface coated with self-adhesive is wound on a rotating shaft 10 in the unwinding roll 7, the free edge of the self-adhesive film 9 penetrates out of the notch of the unwinding roll 7 and penetrates into the notch of the winding roll 8 and is wound on the rotating shaft 10 in the winding roll 8, at the moment, the self-adhesive film 9 can be wound by rotating the rotating shaft 10 of the winding roll 8, and the self-adhesive film 9 positioned between the unwinding roll 7 and the winding roll 8 is positioned right above the box body 1; make it paste the upper surface at box body 1 through the non-setting adhesive film 9 of pushing down this department, alright carry out the shutoff to reagent groove 3 like this, puncture non-setting adhesive film 9 through the syringe needle of pipettor during the sample alright realize the sample, after the sample, owing to be in sterile environment, consequently rotate take-up reel 8 and carry out the rolling switching to the non-setting adhesive film 9 who punctures, it makes its paste the upper surface at box body 1 to push down non-setting adhesive film 9 once more afterwards, thereby realize sealedly, whole can be with comparatively convenient realization sealing process, thereby realize the multiplexing of reagent.
The side position is installed through the hinge is articulated apron 4 behind the upper surface of box body 1, and integrated into one piece has hasp 5 on the front edge of apron 4, and the side position is equipped with the discount 6 that sets up with hasp 5 cooperatees before the upper surface of box body 1, utilizes the apron 4 to turn to push down and realizes the closing cap and push down the laminating to non-setting adhesive film 9 simultaneously.
One end of the rotating shaft 10 of the winding drum 8 is provided with a hexagonal linkage jack 11, and the linkage jack 11 is used for connecting an external rotating device, so that the rotating shaft 10 is driven to rotate and wind.
The self-adhesive film 9 is made of a PP material with a single-side coated with glue.
The utility model discloses a theory of operation is: the self-adhesive film 9 positioned between the unwinding cylinder 7 and the winding cylinder 8 is positioned right above the box body 1; make it paste the upper surface at box body 1 through the non-setting adhesive film 9 of pushing down this department, alright carry out the shutoff to reagent groove 3 like this, puncture non-setting adhesive film 9 through the syringe needle of pipettor during the sample alright realize the sample, after the sample, owing to be in sterile environment, consequently rotate take-up reel 8 and carry out the rolling switching to the non-setting adhesive film 9 who punctures, it makes its paste the upper surface at box body 1 to push down non-setting adhesive film 9 once more afterwards, thereby realize sealedly, whole can be with comparatively convenient realization sealing process, thereby realize the multiplexing of reagent.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (4)
1. A reagent kit for second-generation sequencing nucleic acid extraction comprises a box body with a base, wherein a plurality of reagent grooves which are arranged at intervals and extend downwards are formed in the upper surface of the box body;
the reusable cover sealing device is characterized in that the left side and the right side of the box body are provided with reusable cover sealing devices, each reusable cover sealing device comprises an unwinding barrel and a winding barrel which are arranged on the left side and the right side of the upper end of the box body, the unwinding barrels and the winding barrels are arranged in parallel left and right, each unwinding barrel and each winding barrel comprise a hollow barrel body and a rotating shaft, the rotating shafts are arranged on the inner sides of the barrel bodies and are arranged at intervals with the barrel bodies, bearings are sleeved at the front ends and the rear ends of the rotating shafts, the outer rings of the bearings are fixedly clamped with the barrel bodies, linearly extending notches are formed in the side surfaces of the barrel bodies, and the notches are located; the rotary shaft in the winding drum is wound with a non-setting adhesive film coated with non-setting adhesive on the lower surface, and the free edge of the non-setting adhesive film passes through the notch of the winding drum and penetrates through the notch of the winding drum and is wound on the rotary shaft in the winding drum.
2. The kit for second-generation sequencing nucleic acid extraction according to claim 1, wherein a cover plate is hinged to a rear side position of the upper surface of the box body through a hinge, a buckle is integrally formed on the front edge of the cover plate, and a buckle head matched with the buckle is arranged at a front side position of the upper surface of the box body.
3. The kit for second generation sequencing nucleic acid extraction according to claim 1, wherein a hexagonal linkage jack is processed at one end of the rotating shaft of the winding drum.
4. The kit for second-generation sequencing nucleic acid extraction according to claim 1, wherein the self-adhesive film is made of a PP material with a single-sided adhesive.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021451759.9U CN212741313U (en) | 2020-07-22 | 2020-07-22 | Kit for second-generation sequencing nucleic acid extraction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021451759.9U CN212741313U (en) | 2020-07-22 | 2020-07-22 | Kit for second-generation sequencing nucleic acid extraction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212741313U true CN212741313U (en) | 2021-03-19 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021451759.9U Active CN212741313U (en) | 2020-07-22 | 2020-07-22 | Kit for second-generation sequencing nucleic acid extraction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212741313U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113636195A (en) * | 2021-07-16 | 2021-11-12 | 极研(福建)信息科技有限公司 | Packaging box for embedded intelligent chip |
-
2020
- 2020-07-22 CN CN202021451759.9U patent/CN212741313U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113636195A (en) * | 2021-07-16 | 2021-11-12 | 极研(福建)信息科技有限公司 | Packaging box for embedded intelligent chip |
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