CN212741302U - Improved column type positive phage enrichment device - Google Patents
Improved column type positive phage enrichment device Download PDFInfo
- Publication number
- CN212741302U CN212741302U CN202021207247.8U CN202021207247U CN212741302U CN 212741302 U CN212741302 U CN 212741302U CN 202021207247 U CN202021207247 U CN 202021207247U CN 212741302 U CN212741302 U CN 212741302U
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- cylindrical barrel
- welded
- pipe
- phage enrichment
- threaded connection
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 238000004140 cleaning Methods 0.000 claims abstract description 13
- 238000007789 sealing Methods 0.000 claims description 20
- 239000004005 microsphere Substances 0.000 claims description 18
- 239000000945 filler Substances 0.000 claims description 17
- 230000002093 peripheral effect Effects 0.000 claims description 2
- 239000007921 spray Substances 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 19
- 239000011324 bead Substances 0.000 abstract description 4
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 11
- 235000017491 Bambusa tulda Nutrition 0.000 description 11
- 241001330002 Bambuseae Species 0.000 description 11
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 11
- 239000011425 bamboo Substances 0.000 description 11
- 239000012528 membrane Substances 0.000 description 10
- 230000002349 favourable effect Effects 0.000 description 7
- 239000004793 Polystyrene Substances 0.000 description 6
- 229920002223 polystyrene Polymers 0.000 description 6
- 238000005507 spraying Methods 0.000 description 5
- 230000001276 controlling effect Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 229940125644 antibody drug Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002823 phage display Methods 0.000 description 2
- DGZSVBBLLGZHSF-UHFFFAOYSA-N 4,4-diethylpiperidine Chemical compound CCC1(CC)CCNCC1 DGZSVBBLLGZHSF-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011012 sanitization Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model provides an improved column type positive phage enrichment device, which comprises a cylindrical barrel, a stop block, a water through groove, an adjustable throttle valve, an inner wall cleaning cover structure, a plug-in type phage enrichment frame structure, an adjustable collection vessel placing frame structure, a mounting plate, a connecting rod, a fixed pipe, a clamping bolt, a bearing and an arc-shaped plate, wherein the stop block is welded at the periphery of the lower part of the inner side of the cylindrical barrel; the water passing groove is formed in one side, close to the cylindrical barrel, of the inner part of the stop block; the adjustable throttle valve is in threaded connection with the middle position of the lower part of the cylindrical barrel; the inner wall cleaning cover structure is in threaded connection with the upper part of the inner side of the cylindrical barrel. The utility model has the advantages that: through the setting of connection stay tube and sprinkler bead, this equipment uses up the inner wall of a cylinder to wash, will remain and wash the chemical residue on cylinder inside, prevents that the chemical from remaining and producing the reaction with the chemical when using next time to influence the enrichment result next time.
Description
Technical Field
The utility model belongs to the technical field of it is biological, especially, relate to an improved generation positive phage enrichment device of post formula.
Background
The development of antibody drugs is a hotspot in the field of international biomedical research, and the phage display technology gradually becomes one of important technologies along with the development of the technology in the development of antibody drugs, and the phage display technology has many advantages, can screen out positive antibodies by utilizing a synthetic library under the condition of not passing through body immunity, and can also screen out high-affinity antibodies and the like by utilizing a natural library or a semi-synthetic library.
However, the existing column-type positive phage enrichment device also has the problems that the chemical residues in the equipment are inconvenient to clean after the equipment is used up, the polystyrene microsphere filler is inconvenient to replace by original equipment, and the collecting bottle is inconvenient to position when the collecting bottle is placed, so that the chemicals flow onto the desktop.
Therefore, the invention of an improved column-type positive phage enrichment device is necessary.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides an improved generation positive phage enrichment device of column type to solve current positive phage enrichment device of column type inconvenient after the equipment use up remain the sanitization with the inside chemistry of equipment, former equipment is inconvenient to change polystyrene microballon packing and inconvenient when placing the receiving flask lead to the problem that chemical flows to the desktop to the receiving flask location. An improved cylindrical positive phage enrichment device comprises a cylindrical barrel, a stop block, a water through groove, an adjustable throttle valve, an inner wall cleaning cover structure, a plug-in type phage enrichment frame structure, an adjustable collection dish placing frame structure, a mounting plate, a connecting rod, a fixed pipe, a clamping bolt, a bearing and an arc-shaped plate, wherein the stop block is welded at the periphery of the lower part of the inner side of the cylindrical barrel; the water passing groove is formed in one side, close to the cylindrical barrel, of the inner part of the stop block; the adjustable throttle valve is in threaded connection with the middle position of the lower part of the cylindrical barrel; the inner wall cleaning cover structure is in threaded connection with the upper part of the inner side of the cylindrical barrel; the plug-in type phage enrichment frame structure is plugged in the upper part of the inner side of the cylindrical barrel; the adjustable collecting vessel placing frame structure is connected to the lower part of the cylindrical barrel through bolts; the mounting plate is connected to the upper part of the right side of the cylindrical barrel through a bolt; the connecting rod is welded in the middle of the right side of the mounting plate; the fixed pipe is welded on the right side of the connecting rod; the clamping bolt is in threaded connection with the middle position of the right side inside the fixed pipe; the inner ring of the bearing is connected to the left side of the clamping bolt in an interference manner, and the outer ring is embedded in the middle position of the right side in the arc-shaped plate; the arc-shaped plate is arranged on the right side in the fixed pipe; the inner wall cleaning cover structure comprises a cylinder cover, a threaded connecting pipe, a threaded sealing cover, a circular groove, a waterproof sealing plate, a connecting supporting pipe and a water spraying head, wherein the threaded connecting pipe is welded in the middle of the upper part of the inner side of the cylinder cover; the thread sealing cover is in threaded connection with the upper part of the thread connecting pipe; the circular groove is arranged in the middle of the lower part of the inner side of the cylinder cover; the waterproof sealing plate is connected to the lower part of the inner side of the circular groove through a screw; the connecting supporting pipes are respectively in threaded connection with the periphery of the lower part of the inner side of the waterproof sealing plate; the sprinkler head is welded at the lower part of the connecting support pipe.
Preferably, the plug-in type phage enrichment frame structure comprises a cylindrical frame, a rubber pad, an edge ring, an upper layer of support membrane, a lower layer of support membrane and microsphere fillers, wherein the rubber pad is respectively glued at the periphery of the upper part and the lower part of the cylindrical frame; the edge ring is welded on the periphery of the upper part of the inner side of the cylindrical frame; the upper layer of support film is glued to the lower part of the inner side of the cylindrical frame; the lower layer supporting film is glued at the lower part of the inner side of the cylindrical frame and is arranged at the lower part of the upper layer supporting film; the microsphere filler is arranged between the upper layer support film and the lower layer support film.
Preferably, the adjustable collecting vessel placing frame structure comprises a placing plate, a limiting ring, an adjusting pipe, a fixing rod and an adjusting bolt, wherein the limiting ring is welded at the middle position of the upper part of the placing plate; the adjusting pipes are respectively welded on the left side and the right side of the upper part of the placing plate; the fixed rod is inserted at the upper part of the inner side of the adjusting pipe; the adjusting bolt is in threaded connection with the connecting part of the adjusting pipe and the fixed rod.
Preferably, the periphery of the lower part of the cylinder cover is provided with external threads, and the cylinder cover is in threaded connection with the upper part of the inner side of the cylindrical cylinder.
Preferably, the lower part of the inner side of the threaded connecting pipe is communicated with the middle position of the upper part of the inner side of the cylinder cover.
Preferably, the included angle between the connecting supporting pipe and the water spraying head is set to be seventy-five degrees to eighty degrees, and the lower part of the water spraying head inclines towards the outer side of the cylinder cover.
Preferably, the rubber pads are respectively inserted into the upper part and the lower part of the inner side of the cylindrical barrel, and the cylindrical frame is placed on the upper part of the stop block.
Preferably, the fixing rods are respectively bolted on the left side and the right side of the lower part of the cylindrical barrel.
Preferably, the microsphere filler is polystyrene microsphere filler.
Compared with the prior art, the beneficial effects of the utility model are that:
1. the utility model discloses in, the setting of joint stay tube and sprinkler bead, be favorable to this equipment to wash the inner wall of a cylinder section of thick bamboo after using up, will remain the chemistry on the cylinder section of thick bamboo is remained and washes, prevent that the chemistry from remaining and producing the reaction with the chemical when using next time to influence the enrichment result next time.
2. The utility model discloses in, the setting of cover and thread sealing lid, be favorable to sealing up the inside of a cylinder section of thick bamboo when depositing the device, thereby prevent to influence the device's normal use because the inside production microorganism of an external factor result in a cylinder section of thick bamboo.
3. The utility model discloses in, the setting of cylinder frame and edge ring, be favorable to supporting membrane, lower floor's support membrane and microballon with the upper strata and pack to pull down from a cylinder section of thick bamboo, and then support membrane, lower floor's support membrane and microballon to the upper strata of damage and pack and change.
4. The utility model discloses in, the setting of rubber pad, be favorable to playing sealed effect when inserting a cylinder section of thick bamboo with the cylinder frame, prevent to flow into in the gap of cylinder frame and a cylinder section of thick bamboo to the chemical in the cylinder section of thick bamboo.
5. The utility model discloses in, the setting of placing board and spacing ring, be favorable to placing the collecting bottle in the lower part of adjustable throttle valve accurately, prevent that the opening of collecting bottle is less on the chemical flows the desktop.
6. The utility model discloses in, the setting of regulating tube and dead lever, be favorable to adjusting the distance of placing between board and the adjustable throttle valve lower part according to the size of receiving flask, make it can place multiple receiving flask.
7. The utility model discloses in, the setting of water service tank, be favorable to when the inner wall of a cylinder section of thick bamboo is washd with clear water at joint stay tube and sprinkler bead, with leading-in cylinder section of thick bamboo 1 of clear water and lower part, prevent to have the remaining water of chemistry to stay in the contained angle department of a cylinder section of thick bamboo and dog.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic structural diagram of the inner wall cleaning cover structure of the present invention.
FIG. 3 is a schematic structural diagram of the structure of the plug-in phage enrichment frame of the present invention.
Fig. 4 is a schematic structural view of the adjustable structure of the collecting dish placing rack of the present invention.
In the figure:
1. a cylindrical barrel; 2. a stopper; 3. a water trough; 4. an adjustable throttle valve; 5. an inner wall cleaning cover structure; 51. a cylinder cover; 52. a threaded connection pipe; 53. a thread sealing cover; 54. a circular groove; 55. a waterproof sealing plate; 56. connecting the supporting tube; 57. a sprinkler head; 6. a plug-in phage enrichment scaffold structure; 61. a cylindrical frame; 62. a rubber pad; 63. an edge ring; 64. an upper support film; 65. a lower support film; 66. filling microspheres; 7. an adjustable collecting vessel placing rack structure; 71. placing the plate; 72. a limiting ring; 73. an adjusting tube; 74. fixing the rod; 75. adjusting the bolt; 8. mounting a plate; 9. a connecting rod; 10. a fixed tube; 11. clamping the bolt; 12. a bearing; 13. an arc-shaped plate.
Detailed Description
The invention is further described below with reference to the accompanying drawings:
example (b):
as shown in the attached drawings 1 and 2, an improved column type positive phage enrichment device comprises a cylindrical barrel 1, a stop dog 2, a water trough 3, an adjustable throttle valve 4, an inner wall cleaning cover structure 5, a plug-in type phage enrichment rack structure 6, an adjustable collection dish placing rack structure 7, a mounting plate 8, a connecting rod 9, a fixed pipe 10, a clamping bolt 11, a bearing 12 and an arc-shaped plate 13, wherein the stop dog 2 is welded at the periphery of the lower part of the inner side of the cylindrical barrel 1; the water trough 3 is arranged at one side of the inner part of the stop block 2 close to the cylindrical barrel 1; the adjustable throttle valve 4 is in threaded connection with the middle position of the lower part of the cylindrical barrel 1; the inner wall cleaning cover structure 5 is in threaded connection with the upper part of the inner side of the cylindrical barrel 1; the plug-in type phage enrichment frame structure 6 is plugged in the upper part of the inner side of the cylindrical barrel 1; the adjustable collecting vessel placing frame structure 7 is connected to the lower part of the cylinder 1 through bolts; the mounting plate 8 is connected to the upper part of the right side of the cylindrical barrel 1 through a bolt; the connecting rod 9 is welded in the middle of the right side of the mounting plate 8; the fixed pipe 10 is welded at the right side of the connecting rod 9; the clamping bolt 11 is in threaded connection with the middle position of the right side inside the fixed pipe 10; the inner ring of the bearing 12 is connected to the left side of the clamping bolt 11 in an interference manner, and the outer ring is embedded in the middle position of the right side in the arc-shaped plate 13; the arc-shaped plate 13 is arranged at the right side inside the fixed pipe 10; the inner wall cleaning cover structure 5 comprises a cylinder cover 51, a threaded connecting pipe 52, a threaded sealing cover 53, a circular groove 54, a waterproof sealing plate 55, a connecting supporting pipe 56 and a water spraying head 57, wherein the threaded connecting pipe 52 is welded at the middle position of the upper part of the inner side of the cylinder cover 51; the thread sealing cover 53 is connected to the upper part of the thread connecting pipe 52 in a thread way; the round groove 54 is arranged at the middle position of the inner lower part of the cylinder cover 51; the waterproof sealing plate 55 is connected to the lower part of the inner side of the circular groove 54 through screws; the connection support pipes 56 are respectively in threaded connection with the periphery of the lower part of the inner side of the waterproof sealing plate 55; the sprinkler head 57 is welded at the lower part of the connecting support pipe 56; through the setting of connection stay tube 56 and sprinkler bead 57, this equipment is used up and is washed the inner wall of cylinder 1, will remain and wash the chemical residue on cylinder 1 inside, prevents that chemical residue from producing the reaction with the chemical when using next time to influence the enrichment result next time.
In the above embodiment, as shown in fig. 3, specifically, the plug-in type phage enrichment rack structure 6 includes a cylindrical rack 61, a rubber pad 62, an edge ring 63, an upper support film 64, a lower support film 65 and a microsphere filler 66, wherein the rubber pad 62 is respectively glued to the upper and lower peripheral positions of the cylindrical rack 61; the edge ring 63 is welded at the periphery of the upper part of the inner side of the cylindrical frame 61; the upper layer support film 64 is glued on the lower part of the inner side of the cylindrical frame 61; the lower layer supporting film 65 is glued to the lower part of the inner side of the cylindrical frame 61 and is arranged at the lower part of the upper layer supporting film 64; the microsphere filler 66 is arranged between the upper layer support film 64 and the lower layer support film 65; through the setting of cylinder frame 61 and edge ring 63, pull down upper strata support membrane 64, lower floor's support membrane 65 and microballon packing 66 from the cylinder 1, and then change upper strata support membrane 64, lower floor's support membrane 65 and microballon packing 66 that damage.
As shown in fig. 4, in the above embodiment, specifically, the adjustable collecting dish placing frame structure 7 includes a placing plate 71, a limiting ring 72, an adjusting tube 73, a fixing rod 74 and an adjusting bolt 75, where the limiting ring 72 is welded at an upper middle position of the placing plate 71; the adjusting pipes 73 are respectively welded at the left side and the right side of the upper part of the placing plate 71; the fixed rod 74 is inserted in the upper part of the inner side of the adjusting pipe 73; the adjusting bolt 75 is in threaded connection with the connecting position of the adjusting pipe 73 and the fixed rod 74; by the arrangement of the placing plate 71 and the stopper ring 72, the collection bottle is accurately placed at the lower portion of the adjustable throttle valve 4, and chemicals are prevented from flowing onto a table when the opening of the collection bottle is small.
In the above embodiment, specifically, the periphery of the lower portion of the cylinder cover 51 is provided with external threads, and the cylinder cover 51 is screwed on the upper portion of the inner side of the cylinder 1.
In the above embodiment, specifically, the inner lower portion of the threaded connection pipe 52 communicates with the inner upper middle portion of the cylinder cover 51.
In the above embodiment, specifically, the angle between the connection support pipe 56 and the sprinkler head 57 is set to seventy-five degrees to eighty degrees, and the lower portion of the sprinkler head 57 is inclined toward the outer side of the cylinder cover 51.
In the above embodiment, specifically, the rubber pads 62 are respectively inserted into the upper and lower portions of the inner side of the cylinder 1, and the cylinder frame 61 is placed on the upper portion of the stopper 2.
In the above embodiment, the fixing rods 74 are respectively bolted to the left and right sides of the lower portion of the cylindrical barrel 1.
In the above embodiment, specifically, the microsphere filler 66 specifically uses polystyrene microsphere filler.
Principle of operation
The utility model discloses a theory of operation: when the device is used, firstly, a stand column on a centrifuge tube rack is inserted into the inner side of the fixed tube 10, then the clamping bolt 11 is rotated, the clamping bolt 11 is enabled to move leftwards, the arc-shaped plate 13 is pushed leftwards at the same time, the stand column on the centrifuge tube rack is enabled to be close to a position between the left side of the inner part of the fixed tube 10 and the left side of the arc-shaped plate 13, then the tube cover 51 is unscrewed from the upper part of the cylindrical tube 1, the extending length of the fixed rod 74 on the inner side of the adjusting tube 73 is adjusted according to the height of the collecting bottle, so that the chemical medicine after collecting reaction of the collecting bottle is not influenced, then the adjusting bolt 75 is screwed to fix the relative position of the fixed rod 74 and the adjusting tube 73, then the collecting bottle is placed in the limiting ring 72, the bottle mouth of the collecting bottle is enabled to be opposite to the lower part of the adjustable throttle valve 4, the adjustable throttle valve 4 is firstly, combining the antigen with the polystyrene microsphere filler 66, adding PBS buffer solution into the cylindrical frame 61, adjusting the opening size of the adjustable throttle valve 4, controlling the flow rate of chemicals, washing off the antigen which is not combined with the polystyrene microsphere filler 66, adding the prepared phage antibody display library into the cylindrical frame 61, adjusting the opening size of the adjustable throttle valve 4, controlling the flow rate of chemicals, combining the antibody with the antigen surface combined with the surface of the microsphere filler 66, adding a PBS buffer solution into the cylindrical frame 61, adjusting the opening size of the adjustable throttle valve 4, controlling the flow rate of chemicals, washing off phage which are not combined with the microsphere filler 66, finally adding a Tris glycine buffer solution into the cylindrical frame 61, adjusting the opening size of the adjustable throttle valve 4, controlling the flow rate of chemicals, washing off positive phage, and completing the enrichment of positive phage; when the inner side of the equipment needs to be cleaned, the threaded sealing cover 53 is firstly screwed off, an external water source is in threaded connection with the upper part of the threaded connecting pipe 52, external clean water enters the circular groove 54 through the threaded connecting pipe 52, then precipitation is uniformly sprayed on the inner wall of the cylindrical barrel 1 through the connecting supporting pipe 56 and the water spraying head 57, the water flows downwards along the cylindrical barrel 1 through the water passing groove 3, chemical residues are washed clean, when the upper supporting film 64, the lower supporting film 65 and the microsphere filler 66 need to be cleaned or replaced, the cylindrical frame 61 is pulled out from the cylindrical barrel 1, and the upper supporting film 64, the lower supporting film 65 and the microsphere filler 66 are cleaned or replaced.
Utilize technical scheme, or technical personnel in the field are in the utility model discloses under technical scheme's the inspiration, design similar technical scheme, and reach above-mentioned technological effect, all fall into the utility model discloses a protection scope.
Claims (8)
1. The improved column type positive phage enrichment device is characterized by comprising a cylindrical barrel (1), a stop block (2), a water through groove (3), an adjustable throttle valve (4), an inner wall cleaning cover structure (5), a plug-in type phage enrichment rack structure (6), an adjustable collection dish placing rack structure (7), a mounting plate (8), a connecting rod (9), a fixing pipe (10), a clamping bolt (11), a bearing (12) and an arc-shaped plate (13), wherein the stop block (2) is welded at the periphery of the lower part of the inner side of the cylindrical barrel (1); the water trough (3) is arranged at one side of the inner part of the stop block (2) close to the cylindrical barrel (1); the adjustable throttle valve (4) is in threaded connection with the middle position of the lower part of the cylindrical barrel (1); the inner wall cleaning cover structure (5) is in threaded connection with the upper part of the inner side of the cylindrical barrel (1); the plug-in type phage enrichment frame structure (6) is plugged in the upper part of the inner side of the cylindrical barrel (1); the adjustable collecting vessel placing frame structure (7) is connected to the lower part of the cylindrical barrel (1) through bolts; the mounting plate (8) is connected to the upper part of the right side of the cylindrical barrel (1) through a bolt; the connecting rod (9) is welded in the middle of the right side of the mounting plate (8); the fixed pipe (10) is welded on the right side of the connecting rod (9); the clamping bolt (11) is in threaded connection with the middle position of the right side in the fixed pipe (10); the inner ring of the bearing (12) is connected to the left side of the clamping bolt (11) in an interference manner, and the outer ring is embedded in the middle position of the right side in the arc-shaped plate (13); the arc-shaped plate (13) is arranged on the right side of the inside of the fixed pipe (10); the inner wall cleaning cover structure (5) comprises a cylinder cover (51), a threaded connecting pipe (52), a threaded sealing cover (53), a circular groove (54), a waterproof sealing plate (55), a connecting supporting pipe (56) and a water spray head (57), wherein the threaded connecting pipe (52) is welded in the middle of the upper part of the inner side of the cylinder cover (51); the thread sealing cover (53) is in threaded connection with the upper part of the thread connecting pipe (52); the round groove (54) is arranged in the middle of the lower part of the inner side of the cylinder cover (51); the waterproof sealing plate (55) is connected to the lower part of the inner side of the circular groove (54) through screws; the connecting supporting pipes (56) are respectively in threaded connection with the periphery of the lower part of the inner side of the waterproof sealing plate (55); the sprinkler head (57) is welded at the lower part of the connecting support pipe (56).
2. The improved column type positive phage enrichment device of claim 1, wherein the plug-in type phage enrichment structure (6) comprises a cylindrical frame (61), rubber pads (62), an edge ring (63), an upper support film (64), a lower support film (65) and microsphere fillers (66), wherein the rubber pads (62) are respectively glued at the peripheral positions of the upper part and the lower part of the cylindrical frame (61); the edge ring (63) is welded at the periphery of the upper part of the inner side of the cylindrical frame (61); the upper layer supporting film (64) is glued to the lower part of the inner side of the cylindrical frame (61); the lower layer supporting film (65) is glued to the lower part of the inner side of the cylindrical frame (61) and is arranged at the lower part of the upper layer supporting film (64); the microsphere filler (66) is arranged between the upper layer support film (64) and the lower layer support film (65).
3. The improved column type positive phage enrichment device of claim 1, wherein the adjustable collection dish placing frame structure (7) comprises a placing plate (71), a limiting ring (72), an adjusting tube (73), a fixing rod (74) and an adjusting bolt (75), the limiting ring (72) is welded at the middle position of the upper part of the placing plate (71); the adjusting pipes (73) are respectively welded at the left side and the right side of the upper part of the placing plate (71); the fixed rod (74) is inserted into the upper part of the inner side of the adjusting pipe (73); the adjusting bolt (75) is in threaded connection with the connecting position of the adjusting pipe (73) and the fixing rod (74).
4. The improved column type positive phage enrichment device of claim 1, characterized in that the lower part of the cylinder cover (51) is provided with external threads at the periphery, and the cylinder cover (51) is connected to the upper part of the inner side of the cylinder (1) in a threaded manner.
5. The improved column type positive phage enrichment device of claim 1, wherein the inner lower part of the threaded connection tube (52) is connected to the middle position of the inner upper part of the cover (51).
6. The improved column type positive phage enrichment device of claim 1, wherein the angle between the connection support tube (56) and the sprinkler head (57) is set to seventy-five degrees to eighty degrees, and the lower part of the sprinkler head (57) is inclined toward the outside of the cylinder cover (51).
7. The improved column-type positive phage enrichment device of claim 2, wherein the rubber pads (62) are respectively inserted into the upper and lower parts of the inner side of the cylindrical barrel (1), and the cylindrical frame (61) is placed on the upper part of the stopper (2).
8. The improved column-type positive phage enrichment apparatus of claim 3, wherein the fixing rods (74) are respectively bolted to the left and right sides of the lower part of the cylindrical barrel (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021207247.8U CN212741302U (en) | 2020-06-28 | 2020-06-28 | Improved column type positive phage enrichment device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021207247.8U CN212741302U (en) | 2020-06-28 | 2020-06-28 | Improved column type positive phage enrichment device |
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| Publication Number | Publication Date |
|---|---|
| CN212741302U true CN212741302U (en) | 2021-03-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021207247.8U Active CN212741302U (en) | 2020-06-28 | 2020-06-28 | Improved column type positive phage enrichment device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212741302U (en) |
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2020
- 2020-06-28 CN CN202021207247.8U patent/CN212741302U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: An improved column positive phage enrichment device Effective date of registration: 20211013 Granted publication date: 20210319 Pledgee: Qingdao Jinling Shengtai Financing Guarantee Co.,Ltd. Pledgor: QINGDAO RUNDA BIOTECHNOLOGY Co.,Ltd. Registration number: Y2021370010100 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20221208 Granted publication date: 20210319 Pledgee: Qingdao Jinling Shengtai Financing Guarantee Co.,Ltd. Pledgor: QINGDAO RUNDA BIOTECHNOLOGY Co.,Ltd. Registration number: Y2021370010100 |
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| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: An Improved Column Positive Phage Enrichment Device Effective date of registration: 20221212 Granted publication date: 20210319 Pledgee: Qingdao Jinling Shengtai Financing Guarantee Co.,Ltd. Pledgor: QINGDAO RUNDA BIOTECHNOLOGY Co.,Ltd. Registration number: Y2022370010165 |
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