CN212713484U - Filling device for culture medium of contact disc - Google Patents

Abstract

The utility model provides a contact dish culture medium filling device, include: the device comprises an injector, a communication pipeline, an opening and closing mechanism and an air pump, wherein the injector is used for sucking a culture medium; the syringe includes: the injection cylinder is internally provided with a slidable piston, and the piston is fixedly provided with a push rod extending outwards; the connecting pipeline is arranged on the push rod and is used for realizing the detachable communication between the inner cavity of the injection tube and the output end of the air pump; and the opening and closing mechanism is arranged at the joint of the communicating pipeline and the push rod and is used for opening the communicating pipeline when the second communicating pipeline is communicated with the air pump and closing the second communicating pipeline when the communicating pipeline is separated from the air pump. Contact dish culture medium filling device solves among the prior art because of directly pouring the culture medium into the contact dish and lead to the extravagant and the culture medium is difficult to form the problem of water pearl in the contact dish.

Description

Filling device for culture medium of contact disc

Technical Field

The utility model relates to an equipment for biological cultivation, concretely relates to contact dish culture medium filling device.

Background

The preparation method of the contact dish comprises the following steps: the agar medium is poured directly into the contact dish, and the operation steps have the following disadvantages:

1) the dosage of the agar culture medium is not easy to control, the culture medium is easy to waste too much, and the culture medium is easy to overflow when the dosage is too much;

2) after the culture medium is poured into the contact dish, the culture medium cannot form a convex surface (namely the culture medium cannot form a water bead) due to a small amount of the culture medium and cannot be used;

3) the direct pouring culture medium is easy to spill and adhere to the contact dish, and the loss of the contact dish prepared by the method reaches two tenths.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide a contact dish culture medium filling device solves among the prior art because of directly pouring the culture medium into the contact dish and lead to the culture medium extravagant and the culture medium is difficult to form the beaded problem of water in the contact dish.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a contact dish culture medium filling device, include: the device comprises an injector, a communication pipeline, an opening and closing mechanism and an air pump, wherein the injector is used for sucking a culture medium; the syringe includes: the injection cylinder is internally provided with a slidable piston, and the piston is fixedly provided with a push rod extending outwards; the connecting pipeline is arranged on the push rod and is used for realizing the detachable communication between the inner cavity of the injection tube and the output end of the air pump; and the opening and closing mechanism is arranged at the joint of the communicating pipeline and the push rod and is used for opening the communicating pipeline when the second communicating pipeline is communicated with the air pump and closing the second communicating pipeline when the communicating pipeline is separated from the air pump.

Preferably, the communication line includes: the output end of the air pump is fixedly communicated with one end of an intubation tube, the other end of the intubation tube is inserted into an insertion hole formed in the top of the push rod, the outer wall of the insertion pipe is in buckling fit with the inner wall of the insertion hole, one end of the insertion pipe inserted into the push rod is inserted into one end of the rotating pipe, a first step tightly pressed on the end face of the rotating pipe is formed on the inner wall of the insertion pipe, the other end of the rotating pipe is communicated with one end of the conduction pipe, the other end of the conduction pipe is communicated with the push rod, the conduction pipe cannot rotate relative to the push rod, the conduction pipe and the rotating pipe can move in the vertical direction relative to the push rod, the rotating pipe cannot move in the vertical direction relative to the conduction pipe, and the rotating pipe can rotate, a clamping block protrudes on the outer wall of the rotating pipe, a sliding groove for the clamping block to move in the vertical direction is formed in the push rod, the top of the sliding groove is communicated with an arc-shaped groove, and the clamping block rotates along with the rotating pipe through the arc-shaped groove; the switching mechanism is installed at the conduction pipe low end, and the fixture block is located the spout when intubate and jack inner wall buckle cooperation, and switching mechanism closes conduction pipe low end and the fixture block is located the arc wall after intubate breaks away from with the jack.

Preferably, the opening and closing mechanism includes: first sealing washer, the sunken recess that forms the confession first sealing washer of putting into of push rod bottom surface, first sealing washer is installed at the conduction pipe low side, and conduction pipe low side lateral wall is offered and is used for the intercommunicating pore that will conduct pipe and injection tube inner chamber intercommunication, and first sealing washer is located the intercommunicating pore below, compresses tightly first sealing washer on the recess inner wall with intercommunicating pore and injection tube inner chamber keep apart, and the fixture block is located the arc wall.

Preferably, the inner wall of the groove is fixed to one end of a spring, the other end of the spring is fixed relative to the conduction pipe wall, and the first sealing ring can be kept pressed on the inner wall of the groove under the elastic force of the spring.

Preferably, the rotating pipe wall is provided with an annular groove for inserting the conduction pipe, the inner wall of the annular groove is sunken to form an accommodating groove, a coil spring is convoluted in the accommodating groove, the inner end of the coil spring is fixed with the inner wall of the accommodating groove, the outer end of the coil spring is fixed with the outer wall of the conduction pipe, and the clamping block can be kept in the arc-shaped groove under the elasticity of the coil spring.

Preferably, a second sealing ring is mounted on the first step, and the second sealing ring is pressed against the first step by the rotary pipe.

Preferably, the outer wall of the insertion tube is provided with a clamping hole, the inner wall of the insertion hole protrudes with a clamping protrusion, and the clamping protrusion is matched with the inner wall of the clamping hole in a clamping and buckling manner.

Compared with the prior art, the utility model discloses following beneficial effect has:

through setting up the syringe, realized absorbing the culture medium ration, then inject quantitative culture medium to bank up on the contact surface, because be the injection, consequently culture medium base easily forms the water pearl form on the contact surface, provides a convex surface, conveniently inserts biological species. In order to avoid liquid remaining in the injection tube before using the injector, a communicating pipeline and an air pump are designed, so that the air pump blows air into the injection tube, the remaining liquid is blown away, and the injection amount of the culture medium is prevented from being influenced. The arrangement of the communicating pipeline can influence the suction and injection of the injector, so that the opening and closing mechanism is designed, and the communicating pipeline is separated from the inner cavity of the injection tube after the air pump is detached. And realized also communicating the communicating pipe way with the injection tube inner chamber after the air pump connects, also disconnected communicating pipe way and injection tube inner chamber after the air pump dismantles, made things convenient for the operation, improved operating efficiency.

Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.

Drawings

FIG. 1 is a cross-sectional view of a media filling apparatus for a contact plate;

fig. 2 is an enlarged view of a point a in fig. 1.

Reference numerals: the syringe comprises a syringe 1, a syringe barrel 11, a piston 12, a push rod 13, a second step 130, a communication pipeline 2, a rotary pipe 21, a clamping block 22, a conducting pipe 23, a spring 24, a second sealing ring 25, a coil spring 26, a first step 27, an opening and closing mechanism 3, a cannula 4 and a second step 40.

Detailed Description

In order to make the utility model realize that technical means, creation characteristics, achievement purpose and effect are clearer and easily understand, it is right to combine below the figure and the detailed implementation mode the utility model discloses do further explanation:

as shown in fig. 1 and fig. 2, the utility model provides a contact dish culture medium filling device, include: an injector 1, a communication pipeline 2, an opening and closing mechanism 3 and an air pump (not shown), wherein the injector 1 is used for sucking the culture medium; the syringe 1 comprises: the injection device comprises an injection cylinder 11, a piston 12 and a push rod 13, wherein the injection cylinder 11 is internally provided with the piston 12 capable of sliding, and the piston 12 is fixedly provided with the push rod 13 extending outwards; the communicating pipeline 2 is arranged on the push rod 13, and the communicating pipeline 2 is used for realizing the detachable communication between the inner cavity of the injection tube 11 and the output end of the air pump; an opening and closing mechanism 3 is installed at the connection position of the communication pipeline 2 and the push rod 13, and the opening and closing mechanism 3 is used for opening the communication pipeline 2 when the second communication pipeline 2 is communicated with the air pump and closing the second communication pipeline 2 when the communication pipeline 2 is separated from the air pump.

In order to design a simple structure and provide a basis for the function realization of the opening and closing mechanism 3, the communication pipeline 2 includes: the rotary tube 21, the fixture block 22 and the conduction tube 23, the output end of the air pump is fixedly communicated with one end of an insertion tube 4, the other end of the insertion tube 4 is inserted into a jack formed at the top of the push rod 13, the outer wall of the insertion tube 4 is in snap fit with the inner wall of the jack, the end part of the insertion tube 4 inserted into the push rod 13 is used for inserting one end of the rotary tube 21, the inner wall of the insertion tube 4 is provided with a first step 27 pressed on the end surface of the rotary tube 21, the other end of the rotary tube 21 is communicated with one end of the conduction tube 23, the other end of the conduction tube 23 is used for being communicated with the push rod 13, the conduction tube 23 cannot rotate relative to the push rod 13, the conduction tube 23 and the rotary tube 21 can move in the vertical direction relative to the push rod 13, the rotary tube 21 cannot move in the vertical direction relative to the conduction tube 23, the rotary tube 21 can rotate relative, the top of the sliding groove is communicated with an arc-shaped groove, and the arc-shaped groove is used for enabling the clamping block 22 to rotate along with the rotating pipe 21; the opening and closing mechanism 3 is arranged at the lower end of the conduction pipe 23, the clamping block 22 is positioned in the sliding groove when the insertion pipe 4 is in buckling fit with the inner wall of the insertion hole, and the opening and closing mechanism 3 closes the lower end of the conduction pipe 23 and the clamping block 22 is positioned in the arc-shaped groove after the insertion pipe 4 is separated from the insertion hole. The bottom surface of the conduction pipe 23 is closed.

In order to design the opening/closing mechanism 3 to be engaged with the communication pipe 2, the opening/closing mechanism 3 includes: first sealing washer, the sunken recess that forms the confession first sealing washer of putting into in push rod 13 bottom surface, first sealing washer is installed at conduction pipe 23 low side, and conduction pipe 23 low side lateral wall is offered and is used for the intercommunicating pore that is used for communicating conduction pipe 23 and 11 inner chambers of injection tube, and first sealing washer is located the intercommunicating pore below, compresses tightly first sealing washer on the recess inner wall with intercommunicating pore and 11 inner chambers of injection tube keep apart, and fixture block 22 is located the arc wall.

In order to realize that the first sealing ring can rise automatically and enter into the groove when the insertion pipe 4 is pulled out, the communication between the conduction pipe 23 and the push rod 13 is cut off when the insertion pipe 4 is separated from the insertion hole, the problem that the conduction pipe 23 is sealed by the rising of the insertion pipe 4 needing to be manually matched and the operation is troublesome is avoided, the inner wall of the groove is fixed to one end of a spring 24, the other end of the spring 24 is relatively fixed with the conduction pipe 23, and the first sealing ring can be kept pressed on the inner wall of the groove under the elastic force of the spring 24.

In order to realize that the fixture block 22 automatically rotates to a state of keeping the opening and closing mechanism 3 to cut off the communication between the conduction pipe 23 and the push rod 13, an annular groove for inserting the conduction pipe 23 is formed in the wall of the rotation pipe 21, an accommodating groove is formed in the inner wall of the annular groove in a concave mode, a coil spring 26 is convoluted in the accommodating groove, the inner end of the coil spring 26 is fixed with the inner wall of the accommodating groove, the outer end of the coil spring 26 is fixed with the outer wall of the conduction pipe 23, and the fixture block.

In order to maintain the sealing property after the cannula 4 and the rotary tube 21 are communicated, the second seal ring 25 is mounted on the first step 27, and the second seal ring 25 is pressed against the first step 27 by the rotary tube 21.

In order to realize the buckling fit of the outer wall of the insertion tube 4 and the inner wall of the insertion hole, the outer wall of the insertion tube 4 is provided with a clamping hole, the inner wall of the insertion hole protrudes to form a clamping protrusion, and the clamping protrusion is buckled and matched with the inner wall of the clamping hole.

The first sealing ring and the second sealing ring 25 are both made of elastic rubber materials.

The inner cavity of the syringe 11 is enclosed by the piston 12 and the inner wall of the syringe 11.

The working principle of the embodiment is as follows:

when the air pump is connected, the intubation tube 4 is sleeved outside the rotating tube 21, and the rotating tube 21 extends out of the top of the push rod 13 for a sufficient length, so that the intubation tube 4 is inserted and removed, and the second sealing ring 25 on the first step 27 can be pressed on the rotating tube 21; then, the insertion tube 4 is rotated, because the outer shape of the rotating tube 21 is a cuboid structure, the inner wall of the insertion tube 4 is matched with the rotating tube 21 in shape, the insertion tube 4 cannot rotate relative to the rotating tube 21 after the insertion tube 4 is sleeved outside the rotating tube 21, so that the clamping block 22 is aligned with the sliding groove, and the insertion tube 4 can be inserted into the insertion hole formed in the push rod 13; finally, continue to impel intubate 4 towards the jack in, fixture block 22 slides in the spout, spout inner wall restriction fixture block 22 is rotatory in push rod 13, the cooperation of final card arch and the downthehole wall buckle of draw-in hole, play spacing effect, first sealing washer breaks away from with the recess, through-hole and push rod 13 intercommunication on the conduction tube 23, spring 24 is pulled up, can open the air pump and pump gas in to injection tube 11 this moment, gas blows the interior liquid of injection tube 11, can guarantee injection tube 11 internal drying, avoid leading to the quantity of syringe 1 to push into the interior culture medium of contact dish because of there is liquid to remain, it is needs to have guaranteed that syringe 1 pushes into the culture medium quantity in the contact dish, the condition that the quantity is too little has been avoided appearing.

When the air pump is disassembled, the cannula 4 is pulled out, the clamping protrusion is separated from the clamping hole, the cannula 4 is loosened, the rotating pipe 21 and the conduction pipe 23 are lifted under the elastic force of the spring 24, the clamping block 22 slides in the sliding groove, finally, the clamping block 22 is aligned to the arc-shaped groove, the first sealing ring is tightly pressed on the second step 40130 formed on the inner wall of the groove, so that the first sealing ring can be tightly pressed with the inner wall of the groove, the accommodating space can be provided for the spring 24, subsequently, the cannula 4 is continuously pulled out from the rotating pipe 21, the clamping block 22 rotates under the elastic force of the coil spring 26 after the cannula 4 is separated from the rotating pipe 21, the clamping block 22 is screwed into the arc-shaped groove, the state that the first sealing ring is tightly pressed on the second step 40130 is kept, sealing is realized, and the suction function and the condition that the syringe 1 is influenced by the fact that the syringe 11 is.

Finally, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the present invention can be modified or replaced by other means without departing from the spirit and scope of the present invention, which should be construed as limited only by the appended claims.

Claims (7)

1. A contact plate media filling apparatus, comprising: the device comprises an injector, a communication pipeline, an opening and closing mechanism and an air pump, wherein the injector is used for sucking a culture medium;

the syringe includes: the injection cylinder is internally provided with a slidable piston, and the piston is fixedly provided with a push rod extending outwards;

the connecting pipeline is arranged on the push rod and is used for realizing the detachable communication between the inner cavity of the injection tube and the output end of the air pump;

and the opening and closing mechanism is arranged at the joint of the communicating pipeline and the push rod and is used for opening the communicating pipeline when the second communicating pipeline is communicated with the air pump and closing the second communicating pipeline when the communicating pipeline is separated from the air pump.

2. The apparatus of claim 1, wherein the communication line comprises: the output end of the air pump is fixedly communicated with one end of an intubation tube, the other end of the intubation tube is inserted into an insertion hole formed in the top of the push rod, the outer wall of the insertion pipe is in buckling fit with the inner wall of the insertion hole, one end of the insertion pipe inserted into the push rod is inserted into one end of the rotating pipe, a first step tightly pressed on the end face of the rotating pipe is formed on the inner wall of the insertion pipe, the other end of the rotating pipe is communicated with one end of the conduction pipe, the other end of the conduction pipe is communicated with the push rod, the conduction pipe cannot rotate relative to the push rod, the conduction pipe and the rotating pipe can move in the vertical direction relative to the push rod, the rotating pipe cannot move in the vertical direction relative to the conduction pipe, and the rotating pipe can rotate, a clamping block protrudes on the outer wall of the rotating pipe, a sliding groove for the clamping block to move in the vertical direction is formed in the push rod, the top of the sliding groove is communicated with an arc-shaped groove, and the clamping block rotates along with the rotating pipe through the arc-shaped groove; the switching mechanism is installed at the conduction pipe low end, and the fixture block is located the spout when intubate and jack inner wall buckle cooperation, and switching mechanism closes conduction pipe low end and the fixture block is located the arc wall after intubate breaks away from with the jack.

3. A contact plate media filling apparatus as claimed in claim 2, wherein the opening and closing mechanism comprises: first sealing washer, the sunken recess that forms the confession first sealing washer of putting into of push rod bottom surface, first sealing washer is installed at the conduction pipe low side, and conduction pipe low side lateral wall is offered and is used for the intercommunicating pore that will conduct pipe and injection tube inner chamber intercommunication, and first sealing washer is located the intercommunicating pore below, compresses tightly first sealing washer on the recess inner wall with intercommunicating pore and injection tube inner chamber keep apart, and the fixture block is located the arc wall.

4. A media filling apparatus according to claim 3, wherein the inner wall of the recess is secured to one end of a spring, the other end of the spring being secured against the wall of the conduit, the spring being arranged to maintain the first sealing ring pressed against the inner wall of the recess.

5. The media filling apparatus as claimed in claim 4, wherein the wall of the rotating tube has an annular groove for the insertion of the conduit, the inner wall of the annular groove is recessed to form a receiving groove, the receiving groove is convoluted with a coil spring, the inner end of the coil spring is fixed to the inner wall of the receiving groove, the outer end of the coil spring is fixed to the outer wall of the conduit, and the clamping block is held in the arc-shaped groove under the elastic force of the coil spring.

6. A media filling apparatus as claimed in claim 5, wherein a second sealing ring is mounted on the first step, the second sealing ring being compressed against the first step by the spin tube.

7. The media filling apparatus of claim 6, wherein the outer wall of the insertion tube has a locking hole, and the inner wall of the insertion hole has a locking protrusion, and the locking protrusion is engaged with the inner wall of the locking hole.

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