CN212681043U

CN212681043U - Rotatable multichannel pipettor

Info

Publication number: CN212681043U
Application number: CN202021160084.2U
Authority: CN
Inventors: 王逸舒; 朱书平; 杨勇; 齐道鑫
Original assignee: Weifang Medical University
Current assignee: Weifang Medical University
Priority date: 2020-06-19
Filing date: 2020-06-19
Publication date: 2021-03-12
Anticipated expiration: 2030-06-19

Abstract

The utility model discloses a rotatable formula multichannel pipettor, include: the shell comprises an inner support structure and an outer sheath, and the outer sheath is sleeved outside the inner support structure; the inner supporting structure at least comprises a top plate, a first side plate, a bottom plate and a second side plate; the top plate and the bottom plate are formed by mutually hinging a plurality of parts, so that the shell can be bent towards one side; the connecting frame is formed by mutually hinging a plurality of parts, the middle part of the connecting frame is connected with one end of the pressure lever, and the other end of the pressure lever extends to the interface; the connecting rod of each liquid sucker is connected with the connecting frame; the rotating rod is hollow, a through groove is formed in the side wall of the rotating rod, and the rotating rod is sleeved outside the pressing rod; one end of the rotating rod is positioned at the interface; the knob is sleeved outside the interface, a through groove is formed in the side wall of the interface, and the knob is connected with the rotating rod through the through groove; a clamping piece is arranged between the knob and the outer wall of the interface; and the metal sheets are symmetrically arranged, one end of each metal sheet is connected with the rotating rod, and the other end of each metal sheet is connected with the side plate of the inner support structure.

Description

Rotatable multichannel pipettor

Technical Field

The utility model belongs to the technical field of the microbiological equipment technique and specifically relates to a rotatable formula multichannel pipettor.

Background

In some medical experiments, liquid samples are generally required to be pipetted by using a pipetting sample injector, the conventional pipetting sample injectors are of various types, such as a micro-dispenser, a multi-channel micro-sample injector and the like, the multi-channel micro-sample injector can simultaneously suck and move a plurality of liquid samples, the experimental process can be effectively simplified, and the multi-channel micro-sample injector is widely used.

The use of current multichannel pipettor is generally the porous application of sample to same kind of liquid, because multichannel pipettor can install a plurality of liquid-transfering gun heads simultaneously, and the rifle head becomes one row of setting mostly for the multichannel pipettor has great length, is subject to the opening size that current reagent or cell culture liquid hold the container, and the multichannel pipettor is difficult to directly inhale the appearance from holding the container (like culture dish or major diameter centrifuging tube), or only partial rifle head can stretch into and hold the appearance in the container, influences and move liquid efficiency. In this case, the reagent is poured into a special elongated sample addition well to be pipetted. This increases the number of transfers of liquid and thus the risk of contamination of the reagent.

In addition, since the reagent or the cell culture solution is poured into the sample addition well for sample suction, the volume of the liquid poured into the sample addition well is inevitably larger than the required volume of the liquid, and the reagent is wasted.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem existing in the prior art, the utility model aims at providing a rotatable multichannel pipettor.

In order to realize the purpose of the utility model, one or more embodiments of the utility model disclose the following technical solutions:

a rotatable multichannel pipettor comprising:

the shell comprises an inner support structure and an outer sheath, and the outer sheath is sleeved outside the inner support structure; the inner supporting structure at least comprises a top plate, a first side plate, a bottom plate and a second side plate, and the four parts are connected end to end; the top plate and the bottom plate are formed by mutually hinging a plurality of parts, so that the shell can be bent towards one side; the middle part of the top plate is provided with an interface;

the liquid aspirators are arranged in a straight line, each liquid aspirator comprises a piston part and a connecting rod, the piston part is fixed on the bottom plate, a piston is arranged in the piston part, one end of the connecting rod is connected with the piston, the piston moves to generate vacuum degree in the piston part, and the piston part is provided with a gun head connector;

the connecting frame is formed by mutually hinging a plurality of parts, the middle part of the connecting frame is connected with one end of the pressure lever, and the other end of the pressure lever extends to the interface; the connecting rod of each liquid sucker is connected with the connecting frame;

the rotating rod is hollow, a through groove is formed in the side wall of the rotating rod, and the rotating rod is sleeved outside the pressing rod; one end of the rotating rod is positioned at the interface;

the knob is sleeved outside the interface, a through groove is formed in the side wall of the interface, and the knob is connected with the rotating rod through the through groove; a clamping piece is arranged between the knob and the outer wall of the interface;

the metal sheets are even in number, every two metal sheets are in a group, the two metal sheets in the group are arranged in a central symmetry mode relative to the central shaft of the rotating rod, one end of each metal sheet is connected with the rotating rod, the other end of each metal sheet is connected with the side plate of the inner supporting structure, and the metal sheets in different groups are distributed at different positions of the rotating rod.

Compared with the prior art, the utility model discloses an above one or more technical scheme have gained following beneficial effect:

this roof of pipettor, the bottom plate, the link is formed by a plurality of parts are articulated each other, the rotary rod passes through the sheetmetal and is connected with the curb plate of pipettor, when the rotary rod is rotatory towards a direction, drive the sheetmetal motion, make the sheetmetal take place the spiral deformation, and then the curb plate that drives the casing moves towards inboard, make the casing take place the spiral deformation, and then drive the rifle head and connect and assemble to the middle part, the length of pipettor has been reduced, make it can be in the reagent container (like the culture dish, heavy-calibre centrifuging tube) direct sampling, can reduce the number of times that uses the application of sample groove, and then reduce the contaminated risk of reagent, can save reagent.

The clamping piece between the knob and the outer wall of the interface can limit the knob, so that the knob is prevented from rotating, and the smooth sampling can be effectively guaranteed.

Each aspirator all includes piston portion and connecting rod, and the link of every aspirator is realized through the link to the depression bar, and then realizes the independent setting of every aspirator for can select whole aspirators or select some aspirators to carry out the imbibition.

The through grooves are formed in the rotating rods, so that the continuity of the connecting frame is convenient to realize, and the pressure rod transmits force to each liquid absorber through the connecting frame.

Drawings

The accompanying drawings, which form a part of the specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention without unduly limiting the scope of the invention.

Fig. 1 is a schematic view of an internal structure of a pipette according to an embodiment of the present invention;

fig. 2 is a schematic structural diagram of another embodiment of the present invention;

fig. 3 is a schematic view of a pipette according to an embodiment of the present invention in an expanded state;

fig. 4 is a schematic structural diagram of a screw state of a pipette according to an embodiment of the present invention;

fig. 5 is a cross-sectional view of a metal sheet according to an embodiment of the present invention.

The gun head comprises a connector 1, a connector 2, a limiting block 3, a sliding plate 4, a clamping piece 5, a knob 6, a return spring 7, a positioning plate 8, an outer sheath 9, a top plate 10, a metal sheet 11, a connecting frame 12, a connecting rod 13, a piston part 14, a gun head joint 15, a rotating rod 16, a pressure rod 17, a pressing head 18, a rotary regulator 19 and a pressing block.

Detailed Description

It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments in accordance with the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.

A rotatable multichannel pipettor comprising:

the liquid aspirators are arranged in a straight line, each liquid aspirator comprises a piston part and a connecting rod, the piston part is fixed on the bottom plate, a piston is arranged in the piston part, one end of the connecting rod is connected with the piston, the piston moves to generate vacuum degree in the piston part, and the piston part is provided with a gun head connector; the size of the piston part can be adjusted according to the liquid transferring volume of the liquid transferring device.

the metal sheets are even in number, every two metal sheets are in a group, the two metal sheets in the group are arranged in a central symmetry mode relative to the central shaft of the rotating rod, one end of each metal sheet is connected with the rotating rod, the other end of each metal sheet is connected with the side plate of the inner supporting structure, and the metal sheets in different groups are distributed at different positions of the rotating rod. The cross section of the metal sheet is arc-shaped, the whole metal sheet is strip-shaped, the outer convex surface of the metal sheet is used for being in contact with the rotating rod, and when the rotating rod rotates, acting force is applied to the outer convex surface of the rotating rod, so that the metal sheet deforms.

When the arc-shaped metal sheet is completely straightened, the arc-shaped metal sheet can provide a larger supporting effect for the side plates of the shell, so that the shape of the shell is kept.

The radius of the circle where the arc of the section of the metal sheet is located is 4-7cm, the central angle corresponding to the arc is 30-50 degrees, the curvature of the metal sheet is small, a certain good supporting effect can be achieved on the side plate of the shell, and acting force applied to the knob is moderate when the shell is flattened. In addition, the metal sheet has better elasticity, and all the existing metal or alloy materials with better elasticity can be used for preparing the metal sheet.

The hinge portion of the base plate should be located away from the piston portion.

In some embodiments, a sliding plate, a positioning plate and a return spring are arranged in the interface, the return spring is positioned between the sliding plate and the positioning plate, the positioning rod is fixedly arranged, a through hole is formed in the middle of the positioning rod, and the end part of the pressure rod penetrates through the through hole, is sleeved in the return spring and is connected with the sliding plate.

The joint is connected with a matched liquid-transferring gun, the liquid-transferring gun and the joint can be in threaded connection or can be spliced, as long as the connection between the liquid-transferring gun and the joint can be realized, and the pressing of the liquid-transferring gun can not be influenced, the liquid-transferring gun can be moved downwards according to the volume of liquid to be transferred, the distance for pressing down the liquid-transferring gun is determined, the liquid-transferring gun is pressed downwards, a certain acting force is applied to the sliding plate, the sliding plate is moved downwards, and further the pressing rod is driven to move downwards, the pressing rod drives the piston of each liquid absorber to move downwards for a certain distance in the piston part through the connecting frame, then the gun head is stretched into a reagent, the liquid-transferring gun is loosened, the pressing rod is reset under the elasticity of the reset spring.

During flowing back, loosen the fastener between knob and the joint outer wall, the reverse rotation knob makes the pipettor flatten, then puts into the hole of 96 orifice plates with the rifle head, presses the pipette rifle, can arrange inspiratory reagent down.

Further, the sliding plate is abutted against the inner wall of the interface. To ensure the movement of the slide under pressure.

Furthermore, the inner wall of the interface is also provided with a limiting block, and the limiting block and the reset spring are respectively positioned at two sides of the sliding plate. The limiting block is used for limiting the sliding plate and preventing the sliding plate from sliding out of the interface under the elastic action of the reset spring.

In some embodiments, the through slots on the interface side walls correspond to a central angle of 90-220 °. The knob can be fully rotated to ensure that the shell of the pipettor is fully screwed, and on the other hand, the strength of the interface is required to be ensured.

In some embodiments, one end of the rotating rod abuts against the positioning plate, and the other end of the rotating rod extends to the bottom plate.

Furthermore, a first annular groove is formed in the positioning plate, and the end of the rotating rod extends into the first annular groove and is abutted against the bottom of the first annular groove;

and a second annular groove is formed in the bottom plate, and the other end of the rotating rod extends into the second annular groove and is abutted against the bottom of the second annular groove.

First annular groove and second annular groove can carry on spacingly to the rotary rod, guarantee that the rotary rod is normal rotatory under the effort of knob, prevent the dislocation.

In some embodiments, the clamping piece comprises a sliding block and a clamping pin, the sliding block is movably mounted on a sliding rail on the outer wall of the interface, and the clamping pin is arranged on the sliding block;

the end of the knob is provided with a plurality of grooves, the grooves are positioned on the same circumference, and the grooves and the clamping needles are correspondingly arranged.

When the knob rotates to a certain position, the sliding block moves downwards to drive the clamping needle to move, the clamping needle is inserted into the groove of the knob, the knob can be positioned, and then the metal sheet spiral degree is positioned. When the shell needs to be unfolded, the sliding block is moved upwards, the clamping needle is pulled out from the groove, and the knob is rotated reversely.

In some embodiments, the connecting frame comprises at least a double-layer structure. So as to improve the rigidity of the connecting frame and further ensure that the connecting rod of the liquid suction device moves synchronously along with the pressure rod.

Furthermore, the connecting frame is connected with the side plate of the shell in a sliding mode.

Namely, the end part of the connecting frame is provided with a bulge, the side plate of the shell is provided with a groove, and the bulge is in sliding fit with the groove. The end part of the connecting frame is in sliding connection with the side plate of the shell, and the side plate of the shell plays a good supporting role for the end part of the connecting frame, so that the connecting rod of the liquid suction device can be ensured to move synchronously along with the pressing rod.

In some embodiments, the outer sheath is made of an elastic material. The oversheath can play certain guard action to the inside part of pipettor, avoids the pollutant of environment to get into the pipettor inside.

In some embodiments, the number of aspirators is 4-12.

Examples

As shown in fig. 1, a rotatable multichannel pipette, comprising:

the shell comprises an inner support structure and an outer sheath, and the outer sheath is sleeved outside the inner support structure; the inner supporting structure at least comprises a top plate 9, a first side plate, a bottom plate and a second side plate, and the four parts are connected end to end; the top plate and the bottom plate are formed by mutually hinging a plurality of parts, so that the shell can be bent towards one side; the middle part of the top plate is provided with a connector 1, and the connector 1 is fixedly connected with the top plate 9;

8 liquid absorbers which are arranged in a straight line, wherein each liquid absorber comprises a piston part 13 and a connecting rod 12, the piston part 13 is fixed on the bottom plate, the piston part 13 comprises a cylindrical cavity and a piston, the piston is arranged in the cylindrical cavity, one end of the connecting rod is connected with the piston, the piston moves to generate vacuum degree in the piston part, and the piston part is provided with a gun head joint 14;

the connecting frame 11 is formed by mutually hinging a plurality of parts, the middle part of the connecting frame is connected with one end of a pressure lever 16, and the other end of the pressure lever 16 extends to the interface 1; the connecting rod 16 of each liquid sucker is connected with the connecting frame 11;

the rotating rod 15 is hollow, a through groove is formed in the side wall of the rotating rod, and the rotating rod is sleeved outside the pressing rod; one end of the rotating rod is positioned at the interface; the through grooves on the side walls of the rotating rods 15 are arranged in a matched mode with the connecting frames, and the connecting frames penetrate through the through grooves and can be connected with the pressing rods and the connecting rods of the liquid absorbers.

The knob 5 is sleeved outside the interface 1, a through groove is formed in the side wall of the interface 1, the knob is connected with the rotary rod through the through groove, the through groove is an arc-shaped through groove, and the central angle corresponding to the through groove is 180 degrees; a clamping piece 4 is arranged between the knob 5 and the outer wall of the interface;

the metal sheets are 10 and 2, are arranged in a central symmetry mode relative to the central axis of the rotating rod, one end of each metal sheet is connected with the rotating rod, and the other end of each metal sheet is connected with a side plate of the inner supporting structure. The side of the metal sheet applies pulling force to the side plate, so that the side plate is arranged on the metal sheet; the shell moves inwards under the tension force, so that the shell is spirally deformed. On the other hand, when the case is unfolded, the metal sheet may exert a certain outward supporting force to the side plate, so that the case retains its shape to some extent. The cross section of the metal sheet is arc-shaped, the whole metal sheet is strip-shaped, the outer convex surface of the metal sheet is used for being contacted with the rotating rod, and when the rotating rod rotates, acting force is applied to the outer convex surface of the metal sheet, so that the metal sheet deforms.

The radius of the circle where the arc of the section of the metal sheet is located is 4cm, the central angle corresponding to the arc is 30 degrees, as shown in fig. 5, the metal sheet is made of spring steel, the thickness of the metal sheet is 0.5mm, and the length of the metal sheet is 4 cm.

In order to achieve the consistency of the degree of spiraling of the top plate, the connecting frame and the bottom plate, the hinge positions of the top plate, the connecting frame and the bottom plate are consistent, as shown in fig. 1.

A sliding plate 3, a positioning plate 7 and a return spring 6 are arranged in the joint, the return spring 6 is located between the sliding plate 3 and the positioning plate 7, the positioning plate is fixedly arranged, a through hole is formed in the middle of the positioning plate 7, and the end portion of a pressing rod 16 penetrates through the through hole, is sleeved in the return spring 6 and is connected with the sliding plate 3. The slide plate 3 abuts against the inner wall of the mouthpiece 1. The inner wall of the interface 1 is also provided with a limiting block 2, and the limiting block 2 and the reset spring 6 are respectively positioned at two sides of the sliding plate 3.

The joint is connected with a matched liquid transfer gun, the downward pressing distance of the liquid transfer gun is determined according to the liquid transfer volume required, the liquid transfer gun is pressed downwards, certain acting force is applied to the sliding plate, the sliding plate moves downwards, and then the pressing rod is driven to move downwards, the pressing rod drives the piston of each liquid absorber to move downwards for a certain distance in the piston part through the connecting frame, then the gun head is stretched into a reagent, the liquid transfer gun is loosened, the pressing rod resets under the elastic force of the reset spring, the piston of each liquid absorber resets, and then the reagent is sucked into the gun head, so that liquid absorption is realized.

One end of the rotating rod 15 is abutted against the positioning plate 7, and the other end extends to the bottom plate. A first annular groove is formed in the positioning plate 7, and the end of the rotating rod 15 extends into the first annular groove and is abutted against the bottom of the first annular groove; and a second annular groove is formed in the bottom plate, and the other end of the rotating rod 15 extends into the second annular groove and is abutted against the bottom of the second annular groove. Adopt first annular groove and second annular groove to carry on spacingly to the rotary rod, guarantee that the rotary rod is normal rotatory under the effort of knob, prevent the dislocation.

The clamping piece 4 comprises a sliding block and a clamping needle, the sliding block is movably arranged on a sliding rail on the outer wall of the interface 1, and the clamping needle is arranged on the sliding block; the end part of the knob 5 is provided with a plurality of grooves which are positioned on the same circumference and are arranged corresponding to the clamping needles. When the knob 5 rotates to a certain position, the sliding block moves downwards to drive the clamping needle to move, the clamping needle is inserted into the groove of the knob, the knob can be positioned, and then the metal sheet spiral degree is positioned. When the shell needs to be unfolded, the sliding block is moved upwards, the clamping needle is pulled out from the groove, and the knob is rotated reversely.

The connecting frame 11 is a double-layer structure, each layer of structure is formed by hinging a plurality of parts, and the two layers of structures are connected with each other. The connecting frame is connected with the shell side plate in a sliding mode. Namely, the end part of the connecting frame is provided with a bulge, the side plate of the shell is provided with a groove, and the bulge is in sliding fit with the groove. The end part of the connecting frame is in sliding connection with the side plate of the shell, and the side plate of the shell plays a good supporting role for the end part of the connecting frame, so that the connecting rod of the liquid suction device can be ensured to move synchronously along with the pressing rod.

The oversheath is made by rubber, plays certain guard action to the inside part of pipettor, avoids the pollutant of environment to get into the pipettor inside.

In order to achieve pipetting with this pipette, a push pipetting structure is added in this embodiment, and the overall operation principle is explained in detail as shown in fig. 2.

The pressing liquid-transferring structure is in threaded connection with the interface of the liquid-transferring device.

According to the volume of the reagent to be transferred, the position of the rotary regulator 18 on the pressing rod (the pressing rod is provided with a thread which is matched with the internal thread of the rotary regulator 18) is adjusted, so as to adjust the distance that the pressing liquid transfer structure can be pressed down when in pressing.

After the regulation finishes, move fastener 4 upwards, make the card needle extract from knob 5, rotatory knob 5 stirs the rotary rod 15 and rotates, and the one end that drives sheetmetal 10 is rotatory, forms the heliciform, and two curb plates that the rotation of sheetmetal 10 drove the casing move to the inboard, and roof, link and bottom plate take place the spiral deformation under the pressure of curb plate, and then drive the aspirator spiral and arrange. After the adjustment is finished, the clamping piece 4 is moved downwards, the clamping needle is inserted into the groove in the knob 5, the knob 5 is positioned, the metal sheet 10 is kept in a spiral state, the overall shape of the pipette is shown in fig. 4, and the maximum distance between the tips of the pipettes in the spiral state is 5 cm.

A gun head is installed on an adjusted liquid transfer device, a pressing head 17 is pressed downwards, the pressing head 17 applies pressure to a sliding plate 3 through a pressing block 19, the sliding plate moves downwards and drives a pressing rod 16 to move downwards, the pressing rod 16 moves downwards under the action of the sliding plate 3 and applies downward acting force to connecting rods of 8 liquid aspirators through a connecting frame 11, so that the connecting rods of the liquid aspirators move downwards and press pistons to move downwards. Then, 8 gun heads simultaneously extend into a culture dish with the diameter of 6cm and containing cell culture solution, the pressing head 17 is loosened, the sliding plate 3 is reset under the action of a reset spring, the pistons in the liquid aspirators are reset, and then reagents with certain volume are aspirated into the gun heads.

When the sucked reagent needs to be discharged into the 96-well plate, the clamping piece 4 is slid upwards to pull the clamping needle out of the knob 5, the knob 5 is rotated reversely to reset the metal sheet 10, the whole pipettor is unfolded, as shown in fig. 3, then the clamping piece 4 is slid downwards to insert the clamping needle into the corresponding groove of the knob, and the position of the knob is located. And finally, respectively placing the gun heads corresponding to the holes of the 96-hole plate, pressing the pressing head 17 downwards, and discharging the liquid in the gun heads into the holes.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A rotatable multichannel pipettor, its characterized in that: the method comprises the following steps:

2. The rotatable multichannel pipette of claim 1, wherein: the connector is internally provided with a sliding plate, a positioning plate and a return spring, the return spring is positioned between the sliding plate and the positioning plate, the positioning rod is fixedly arranged, the middle part of the positioning rod is provided with a through hole, and the end part of the pressure rod penetrates through the through hole, is sleeved in the return spring and is connected with the sliding plate.

3. The rotatable multichannel pipette of claim 2, wherein: the sliding plate is abutted against the inner wall of the interface.

4. The rotatable multichannel pipette of claim 2, wherein: the inner wall of the interface is also provided with a limiting block, and the limiting block and the reset spring are respectively positioned at two sides of the sliding plate.

5. The rotatable multichannel pipette of claim 1, wherein: the central angle corresponding to the through groove on the side wall of the interface is 90-180 degrees.

6. The rotatable multichannel pipette of claim 1, wherein: one end of the rotating rod is abutted against the positioning plate, and the other end of the rotating rod extends to the bottom plate.

7. The rotatable multichannel pipette of claim 6, wherein: a first annular groove is formed in the positioning plate, and the end of the rotating rod extends into the first annular groove and is abutted against the bottom of the first annular groove;

8. The rotatable multichannel pipette of claim 1, wherein: the clamping piece comprises a sliding block and a clamping needle, the sliding block is movably arranged on the sliding rail on the outer wall of the interface, and the clamping needle is arranged on the sliding block;

9. The rotatable multichannel pipette of claim 1, wherein: the connecting frame at least comprises a double-layer structure.

10. The rotatable multichannel pipette of claim 9, wherein: the connecting frame is connected with the shell side plate in a sliding mode.