CN212533016U

CN212533016U - Device for preparing cell suspension from autologous skin tissue

Info

Publication number: CN212533016U
Application number: CN202021092177.6U
Authority: CN
Inventors: 王忠坤
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-06-12
Filing date: 2020-06-12
Publication date: 2021-02-12
Anticipated expiration: 2030-06-12

Abstract

The utility model discloses a cell suspension device is prepared to autologous skin tissue, including preparation cell suspension device body and apron, the top of preparation cell suspension device body is equipped with the thermometer, the bilateral symmetry of preparation cell suspension device body is equipped with first connecting plate, first connecting plate and preparation cell suspension device body fixed connection, the bilateral symmetry of apron is equipped with the second connecting plate, second connecting plate and apron fixed connection, the quad slit has been seted up to one side of first connecting plate, one side fixedly connected with pinion rack of second connecting plate, quad slit and pinion rack cooperate. A autologous skin tissue preparation cell suspension device, change the traditional connected mode between preparation cell suspension device body and the apron for apron and preparation cell suspension device body pass through the inseparable link of sealing ring and are in the same place, have improved the leakproofness of whole device.

Description

Device for preparing cell suspension from autologous skin tissue

Technical Field

The utility model relates to a technical field is prepared to the cell suspension, in particular to autologous skin tissue preparation cell suspension device.

Background

The device for preparing cell suspension from autologous skin tissue is a disposable autologous cell collection device powered by a battery. It comprises 4 parts: temperature controlled containers, buffer containers, filters and a platform for manipulation containing a specially prepared enzymatic cell dissociation and activation solution, as well as buffers, sterile surgical instruments and actuators. Adding special enzyme solution into the temperature control container, and decomposing epidermis at 37 deg.C. The buffer container is used for containing a neutralization reagent. The operation platform is used for preparing the epidermal cell suspension. The filter is used for filtering substances such as scurf and the like in the cell suspension. The filtered cell suspension is extracted and can be immediately applied to the prepared wound surface. The cell suspension contains a mixed cell group decomposed from a donor skin chip, mainly comprising keratinocytes and fibroblasts, however, if the package is damaged or has damage signs, the cell suspension preparation device for autologous skin tissues cannot be used, the requirement on the sealing performance of the device is higher, the cell suspension preparation device and the cover plate are generally connected through a buckle, although the sealing performance of the device is improved by the outer package, the sealing performance of the device is poor.

SUMMERY OF THE UTILITY MODEL

The main object of the utility model is to provide a cell suspension device is prepared to autologous skin tissue, can effectively solve the problem in the background art.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a cell suspension device prepared from autologous skin tissues comprises a cell suspension device body and a cover plate, wherein a thermometer is arranged at the top end of the cell suspension device body, first connecting plates are symmetrically arranged on two sides of the cell suspension device body and fixedly connected with the cell suspension device body, second connecting plates are symmetrically arranged on two sides of the cover plate and fixedly connected with the cover plate, a square hole is formed in one side of each first connecting plate, a toothed plate is fixedly connected to one side of each second connecting plate and matched with the square hole, a driving cavity and a movable cavity are formed in the first connecting plate, the inner wall of one side of the driving cavity is communicated with the inner wall of one side of the movable cavity, a rotating shaft is arranged in the driving cavity, and one end of the rotating shaft is connected with a bearing positioned in the movable cavity, the outside of pivot is equipped with the teeth of a cogwheel with pinion rack matched with, just one side inner wall of drive cavity is linked together with one side inner wall of quad slit, first recess has been seted up to one side of first connecting plate, one side inner wall of drive cavity is linked together with one side inner wall of first recess, be equipped with the gear in the first recess, the gear passes through elastic component with the pivot and is connected, top one side of preparation cell suspension device body is equipped with reddish yellow green pilot lamp and aseptic technique platform, the top of preparation cell suspension device body is equipped with purpose-made enzyme hole, buffer solution hole and filter hole.

Preferably, the elastic assembly comprises a second groove arranged at one end of the rotating shaft, a pull rod is arranged in the second groove, one end of the pull rod is fixedly connected with one side of the gear, and the other end of the pull rod is connected with the inner wall of one side of the second groove through a spring.

Preferably, the inner walls of the two sides of the second groove are provided with sliding grooves, sliding blocks are arranged in the sliding grooves, and one side of each sliding block is fixedly connected with one side of each pull rod.

Preferably, the inner wall of the first groove is provided with gear teeth matched with the gear, and one side of the gear, far away from the first connecting plate, is fixedly connected with a pull ring.

Preferably, one side of the cover plate is fixedly connected with a sealing ring.

Compared with the prior art, the utility model discloses following beneficial effect has: through the cooperation that sets up first connecting plate, second connecting plate, quad slit, pinion rack, drive chamber, activity cavity, pivot, bearing, first recess, gear, pull ring, sealing ring and elastic component, change the connected mode between traditional preparation cell suspension device body and the apron for apron and preparation cell suspension device body pass through the inseparable connection of sealing ring and are in the same place, have improved the leakproofness of whole device.

Drawings

FIG. 1 is a schematic diagram of the overall structure of a device for preparing cell suspension from autologous skin tissue according to the present invention;

FIG. 2 is a schematic view of the combination structure of the cell suspension preparing device body and the cover plate of the cell suspension preparing device for autologous skin tissue of the present invention;

FIG. 3 is a schematic structural view of the combination of the toothed plate and the square hole of the device for preparing cell suspension from autologous skin tissue of the present invention;

fig. 4 is a partially enlarged schematic view of a portion a of fig. 3.

In the figure: 1. preparing a cell suspension device body; 2. a cover plate; 3. a thermometer; 4. a first connecting plate; 5. a second connecting plate; 6. a square hole; 7. a toothed plate; 8. a drive chamber; 9. a movable chamber; 10. a rotating shaft; 11. a bearing; 12. a first groove; 13. a gear; 14. a pull rod; 15. a pull ring; 16. a second groove; 17. a spring; 18. a chute; 19. a slider; 20. a seal ring; 21. specially preparing enzyme holes; 22. a red, yellow and green indicator light; 23. a buffer liquid hole; 24. an aseptic worktop; 25. a filter aperture.

Detailed Description

In order to make the technical means, creation features, achievement purposes and functions of the present invention easy to understand, the present invention is further described below with reference to the following embodiments.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front end", "rear end", "both ends", "one end", "the other end" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element to which the reference is made must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, and for example, "connected" may be either fixedly connected or detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

As shown in FIGS. 1 to 4, a device for preparing cell suspension from autologous skin tissue comprises a device body 1 for preparing cell suspension and a cover plate 2, wherein a thermometer 3 is arranged at the top end of the device body 1 for preparing cell suspension, first connecting plates 4 are symmetrically arranged on two sides of the device body 1 for preparing cell suspension, the first connecting plates 4 are fixedly connected with the device body 1 for preparing cell suspension, second connecting plates 5 are symmetrically arranged on two sides of the cover plate 2, the second connecting plates 5 are fixedly connected with the cover plate 2, a square hole 6 is arranged on one side of the first connecting plate 4, a toothed plate 7 is fixedly connected with one side of the second connecting plate 5, the square hole 6 is matched with the toothed plate 7, a driving chamber 8 and a movable chamber 9 are arranged in the first connecting plate 4, the inner wall of one side of the driving chamber 8 is communicated with the inner wall of one side of the movable chamber 9, a rotating shaft 10 is arranged in the driving chamber 8, one, gear teeth matched with the toothed plate 7 are arranged outside the rotating shaft 10, the inner wall of one side of the driving cavity 8 is communicated with the inner wall of one side of the square hole 6, one side of the first connecting plate 4 is provided with a first groove 12, the inner wall of one side of the driving cavity 8 is communicated with the inner wall of one side of the first groove 12, a gear 13 is arranged in the first groove 12, the gear 13 is connected with the rotating shaft 10 through an elastic component, one side of the top end of the cell suspension preparation device body 1 is provided with a red, yellow and green indicator lamp 22 and a sterile operating table 24, and the top end of the cell suspension preparation device body 1 is provided with a specially-made enzyme hole 21, a buffer solution hole;

the elastic component comprises a second groove 16 arranged at one end of the rotating shaft 10, a pull rod 14 is arranged in the second groove 16, one end of the pull rod 14 is fixedly connected with one side of the gear 13, and the other end of the pull rod 14 is connected with the inner wall of one side of the second groove 16 through a spring 17; the inner walls of the two sides of the second groove 16 are both provided with sliding grooves 18, sliding blocks 19 are arranged in the sliding grooves 18, and one side of each sliding block 19 is fixedly connected with one side of the pull rod 14; the inner wall of the first groove 12 is provided with gear teeth matched with the gear 13, and one side of the gear 13 far away from the first connecting plate 4 is fixedly connected with a pull ring 15; a sealing ring 20 is fixedly connected to one side of the cover plate 2.

It should be noted that, the utility model relates to a cell suspension device is prepared from autologous skin tissue, when using, the pinion rack 7 aligns square hole 6, and then drive pinion rack 7 to insert in square hole 6, when pinion rack 7 contacts with pivot 10, pulling pull ring 15, make gear 13 break away from first recess 12, and then pull rod 14 slides in second recess 16, spring 17 is in the tensile state, through rotatory gear 13, and then pull rod 14 drives pivot 10 to rotate through slider 19, through the meshing of pivot 10 and pinion rack 7, drive pinion rack 7 moves down, and then second connecting plate 5 drives apron 2 to be close to preparation cell suspension device body 1 and move, finally preparation cell suspension device body 1 and apron 2 closely laminating together through sealing ring 20, after the rotation, loosen pull ring 15, and then spring 17 drives pull rod 14 to move, pull rod 14 drive gear 13 slides into first recess 12, through the meshing of first recess 12 and gear 13, avoid pull rod 14 rotatory, and then the position of the spacing pivot 10 of pull rod 14, avoid pivot 10 to rotate, and then inject the position of pinion rack 7 for apron 2 can not change the position of preparing cell suspension device body 1 relatively, and then avoid the leakproofness to descend, when concrete experiment operation, takes off the small bottle top cap of mark for the enzyme, can see the injection diaphragm. The membrane was wiped with sterile alcohol and dried. A sharp sterile needle was attached to a 10 ml syringe and filled with sterile water. The bottle filled with the sterile water was filled with the whole amount of the enzyme. No buffer was used at this step, otherwise the enzyme activity could be inhibited. The vial was gently tilted to mix the solution until completely dissolved. Do not shake and use care to avoid foaming. The dissolved enzyme was aspirated back into the syringe. Using a sterile procedure, the enzyme in the syringe is completely injected into the purpose-made enzyme well 21. The cap of the vial labeled buffer was removed. The vial septum was wiped with sterile alcohol and air dried. A sharp sterile needle was attached to a new 10 ml syringe and filled with sterile water, the buffer in the syringe was filled into the buffer hole 23, and the cell strainer filter hole 25 was checked for integrity.

The basic principles and the main features of the invention and the advantages of the invention have been shown and described above. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A cell suspension device is prepared to autologous skin tissue which characterized in that: comprises a cell suspension preparation device body (1) and a cover plate (2), wherein a thermometer (3) is arranged at the top end of the cell suspension preparation device body (1), first connecting plates (4) are symmetrically arranged on two sides of the cell suspension preparation device body (1), the first connecting plates (4) are fixedly connected with the cell suspension preparation device body (1), second connecting plates (5) are symmetrically arranged on two sides of the cover plate (2), the second connecting plates (5) are fixedly connected with the cover plate (2), square holes (6) are formed in one side of the first connecting plates (4), toothed plates (7) are fixedly connected with one side of the second connecting plates (5), the square holes (6) are matched with the toothed plates (7), a driving cavity (8) and a movable cavity (9) are arranged in the first connecting plates (4), and the inner wall of one side of the driving cavity (8) is communicated with the inner wall of one side of the movable cavity (9), be equipped with pivot (10) in drive cavity (8), the one end of pivot (10) is connected with bearing (11) that are located movable cavity (9), the outside of pivot (10) is equipped with the teeth of a cogwheel with pinion rack (7) matched with, just one side inner wall of drive cavity (8) is linked together with one side inner wall of quad slit (6), first recess (12) have been seted up to one side of first connecting plate (4), one side inner wall of drive cavity (8) is linked together with one side inner wall of first recess (12), be equipped with gear (13) in first recess (12), gear (13) are connected through elastic component with pivot (10), top one side of preparation cell suspension device body (1) is equipped with reddish yellow green pilot lamp (22) and aseptic operation platform (24), the top of preparation cell suspension device body (1) is equipped with purpose-made enzyme hole (21), A buffer hole (23) and a filter hole (25).

2. The device for preparing the cell suspension from the autologous skin tissue according to claim 1, wherein: elastic component is including offering second recess (16) in pivot (10) one end, be equipped with pull rod (14) in second recess (16), one side fixed connection of one end and gear (13) of pull rod (14), the other end of pull rod (14) passes through spring (17) with one side inner wall of second recess (16) and is connected.

3. The device for preparing the cell suspension from the autologous skin tissue according to claim 2, wherein: sliding grooves (18) are formed in the inner walls of the two sides of the second groove (16), a sliding block (19) is arranged in each sliding groove (18), and one side of each sliding block (19) is fixedly connected with one side of each pull rod (14).

4. The device for preparing the cell suspension from the autologous skin tissue according to claim 1, wherein: the inner wall of the first groove (12) is provided with gear teeth matched with the gear (13), and one side, far away from the first connecting plate (4), of the gear (13) is fixedly connected with a pull ring (15).

5. The device for preparing the cell suspension from the autologous skin tissue according to claim 1, wherein: and one side of the cover plate (2) is fixedly connected with a sealing ring (20).