CN212527831U - Quick rubber cutting ruler - Google Patents

Abstract

A quick rubber cutting ruler relates to the field of experimental appliances. Comprises a rubber ruler body, a supporting seat and a movable shearing device; the two supporting seats are respectively positioned at the two ends of the bottom of the rubber ruler body; the rubber ruler body is provided with a connecting groove which is communicated up and down along the length direction, and the movable shearing device is arranged on the rubber ruler body in a sliding manner; the movable shearing device comprises a blade, a movable block, a pressing unit and a return spring; the inside of movable block is equipped with a hollow cavity, and the lower part of pressing the unit is located the cavity, and reset spring overlaps the lower part of locating the unit of pressing, and reset spring's one end top supports the unit of pressing, and reset spring's other end top supports the movable block, presses the bottom of unit and connects the blade, and wherein press the unit and press down, makes reset spring compress, and the blade moves down and stretches out cavity and spread groove, loosens after pressing the unit, and reset spring rebounds, and the blade is withdrawed to the cavity. Simple structure, convenient operation can cut the glue fast through pressing the button and removing, conveniently tailors the PVDF membrane.

Description

Quick rubber cutting ruler

Technical Field

The utility model relates to an experimental apparatus field especially relates to a quick rubber cutting chi.

Background

Western immunoblotting (Western Blot) is a commonly used experimental procedure in molecular biology, biochemistry and immunogenetics. Western blotting is a method in which the electrophoretically separated fractions are transferred from a gel to a solid support (NC membrane or PVDF membrane) and an antigen-antibody immunoreaction is carried out with an epitope of a target protein attached to the solid support using an antibody as a probe. The method can be used for identifying and semi-quantitatively analyzing a specific protein in a protein mixture (namely, a total protein mixture) extracted from cells or tissues, and aims to identify the type (such as subtype, polymer, splicing body and the like) of the specific protein and the change of the protein expression quantity.

Western blotting is roughly divided into the following 7 steps: protein extraction, protein quantification, SDS-PAGE electrophoresis, electrotransformation, blocking, antigen-antibody immunoreaction and protein detection, wherein the electrotransformation is a crucial step. Upon electroporation, the electric field lines are perpendicular to the SDS-PAGE gel and the membrane. Therefore, the proteins in the SDS-PAGE gel are transferred to the membrane closely attached to the gel under the action of the electric field, and are finally adsorbed on the surface of the membrane. Before membrane transfer, SDS-PAGE gel needs to be processed, namely the position of the target protein is judged according to the position of a marker, and the adhesive tape where the target protein is located is cut off. Generally, a plurality of target proteins to be detected exist in a Western Blot experiment, and if the molecular weight difference of the target proteins is large, the membrane transfer time of the proteins with different molecular weights is different, so that the gel is cut according to the molecular weight of the target proteins, and the membrane transfer time is determined. In practical situations, the cutting of the gel is usually performed by using a gel cutting ruler, but the gel cutting ruler is short, the gel cannot be cut at one time, and the cutting of the gel in several times can lead to the wrong cutting and even the target protein is cut off. And the glue cutting ruler is slightly long in glue cutting time, so that the SDS-PAGE glue is easy to crack, and the experiment fails. Therefore, how to rapidly and accurately process SDS-PAGE gel is a problem to be solved urgently in immunoblotting tests.

Disclosure of Invention

An object of the utility model is to solve the above-mentioned problem among the prior art, provide a quick rubber cutting chi, it is fast to cut the jiao, and can accurately cut the jiao.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a quick glue cutting ruler comprises a glue ruler body, a supporting seat and a movable shearing device; the two supporting seats are respectively positioned at two ends of the bottom of the rubber ruler body so as to support the rubber ruler body to be away from the horizontal plane of the bottom by a certain distance; the rubber ruler body is provided with a connecting groove which is communicated up and down along the length direction, and the movable shearing device is arranged on the rubber ruler body in a sliding manner; the movable shearing device comprises a blade, a movable block, a pressing unit and a return spring; the inside of movable block is equipped with a hollow cavity, the lower part of pressing the unit is located the cavity, reset spring cover locates the lower part of pressing the unit, and reset spring's one end top supports the unit of pressing, and reset spring's other end top supports the movable block, presses the bottom of unit and connects the blade, and wherein presses the unit and press down, makes reset spring compress, and the blade moves down and stretches out cavity and spread groove, loosens after pressing the unit, and reset spring rebounds, and the blade is withdrawed to the cavity.

The pressing unit comprises a guide post, the guide post is positioned in the cavity, the reset spring is sleeved on the guide post, a stopping part extending outwards is arranged at the upper part of the guide post, and one end of the reset spring abuts against the stopping part; the lower part of the cavity of the moving block is provided with a stop part extending towards the center of the cavity, and the other end of the return spring abuts against the stop part.

The top of the cavity of the moving block is provided with a limiting part extending towards the center of the cavity, so that the limiting part abuts against the stopping part to prevent the pressing unit from being separated from the cavity.

The rubber ruler is characterized in that track grooves are formed in the top of the rubber ruler body along two sides of the length direction, and inserting plates inserted into the track grooves extend outwards from the bottom of the moving block, so that the moving block can move back and forth along the track grooves of the rubber ruler body.

The top of the pressing unit is provided with a button, and the top surface of the button is provided with anti-skidding convex points or anti-skidding stripes.

The supporting seat is fixedly connected with the rubber ruler body or integrally formed.

The blade is detachably connected with the bottom of the pressing unit.

The rubber ruler body is marked with length scales.

The length of the graduated scale of the rubber ruler body is 10 cm.

The height of the supporting seat is 1 cm.

Compared with the prior art, the utility model discloses technical scheme obtains beneficial effect is:

the utility model has simple structure and convenient operation, can cut the glue rapidly by pressing the button and moving, and the glue ruler is provided with scales, so that the size of the glue can be measured while cutting the glue, the PVDF film can be cut conveniently, the experimental time is further shortened, and the experimental efficiency is improved; in addition, the rubber cutting ruler can be retracted when not used, and the safety is high.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is an enlarged view of portion A of FIG. 1;

fig. 3 is a schematic structural diagram of a mobile shearing device.

Reference numerals: the rubber ruler comprises a rubber ruler body 1, a supporting seat 2, a blade 3, a moving block 4, a pressing unit 5, a return spring 6, a connecting groove 11, a track groove 12, a limiting part 41, an inserting plate 42, a stop part 43, a button 51, a guide column 52 and a stop part 521.

Detailed Description

In order to make the technical problem, technical solution and beneficial effects to be solved by the present invention clearer and more obvious, the following description is made in detail with reference to the accompanying drawings and embodiments.

As shown in fig. 1 to 3, the present embodiment includes a tape measure body 1, a support base 2, and a movable shearing device;

the two supporting seats 2 are respectively positioned at two ends of the bottom of the rubber ruler body 1 to support the rubber ruler body 1 to be away from the horizontal plane of the bottom by a certain distance;

the rubber ruler body 1 is provided with a connecting groove 11 which is communicated up and down along the length direction;

the movable shearing device is arranged on the rubber ruler body 1 in a sliding mode and comprises a blade 3, a moving block 4, a pressing unit 5 and a return spring 6;

a hollow cavity is arranged inside the moving block 4; the pressing unit 5 includes a guide post 52 and a button 51; the button 51 is arranged on the top of the guide post 52, and anti-skid bumps or anti-skid stripes are arranged on the top surface of the button 51; the guide post 52 is positioned in the cavity, the reset spring 6 is sleeved on the guide post 52, a stopping portion 521 extending outwards is arranged at the upper part of the guide post 52, and one end of the reset spring 6 abuts against the stopping portion 521; the lower part of the cavity of the moving block 4 is provided with a stop part 43 extending towards the center of the cavity, and the other end of the return spring 6 abuts against the stop part 43; the bottom of the pressing unit 5 is connected with the blade 3;

wherein, pressing unit 5 pushes down, makes reset spring 6 compress, and blade 3 moves down and stretches out cavity and spread groove 11, loosens pressing unit 5 after, reset spring 6 rebounds, and blade 3 withdraws to the cavity.

More specifically, the top of the cavity of the moving block 4 is provided with a limiting portion 41 extending toward the center of the cavity, so that the limiting portion 41 abuts against the stopping portion 521 to prevent the pressing unit 5 from coming off the cavity.

In this embodiment, track grooves 12 are formed in the top of the rubber ruler body 1 along two sides of the length direction, and a plug board 42 inserted into the track grooves 12 is arranged at the bottom of the moving block 4 in an outward extending manner, so that the moving block 4 moves back and forth along the track grooves 12 of the rubber ruler body 1.

The blade 3 is detachably connected with the bottom of the pressing unit 5 so as to replace the blade 3.

The utility model discloses a whole product material is ordinary plastics material, glue and be marked with the length scale on the chi body 1, the scale is long for 10cm, and the width of gluing chi body 1 is 2cm, and thickness is 0.2 cm. The supporting seat 2 is a cuboid, and is 0.5cm long, 2cm wide and 1cm high in size. The supporting seat 2 and the rubber ruler body 1 can be fixedly connected or integrally formed.

The utility model discloses a use method as follows:

when SDS-PAGE glue is processed, the position of target protein is determined according to a marker, the glue ruler is placed at the position needing to be cut, the blade 3 extends out of the glue ruler body 1 by pressing the button 51, the moving block 4 is slid at the same time, the blade 3 starts to cut the glue, and the blade 3 is retracted after the button 51 is released. And vertically placing the rubber ruler after the transverse cutting is finished, and vertically cutting the rubber. Use the utility model discloses surely glue and can guarantee to cut fast, accurately and glue to glue and have the scale on the chi, just can measure gluey size when cutting gluey, conveniently tailor the PVDF membrane, further shorten the experimental time.

Claims (10)

1. The utility model provides a cut rubber ruler fast which characterized in that: comprises a rubber ruler body, a supporting seat and a movable shearing device; the two supporting seats are respectively positioned at two ends of the bottom of the rubber ruler body so as to support the rubber ruler body to be away from the horizontal plane of the bottom by a certain distance; the rubber ruler body is provided with a connecting groove which is communicated up and down along the length direction, and the movable shearing device is arranged on the rubber ruler body in a sliding manner; the movable shearing device comprises a blade, a movable block, a pressing unit and a return spring; the inside of movable block is equipped with a hollow cavity, the lower part of pressing the unit is located the cavity, reset spring cover locates the lower part of pressing the unit, and reset spring's one end top supports the unit of pressing, and reset spring's other end top supports the movable block, presses the bottom of unit and connects the blade, and wherein presses the unit and press down, makes reset spring compress, and the blade moves down and stretches out cavity and spread groove, loosens after pressing the unit, and reset spring rebounds, and the blade is withdrawed to the cavity.

2. A quick cutting rule according to claim 1, wherein: the pressing unit comprises a guide post, the guide post is positioned in the cavity, the reset spring is sleeved on the guide post, a stopping part extending outwards is arranged at the upper part of the guide post, and one end of the reset spring abuts against the stopping part; the lower part of the cavity of the moving block is provided with a stop part extending towards the center of the cavity, and the other end of the return spring abuts against the stop part.

3. A quick cutting rule according to claim 2, wherein: the top of the cavity of the moving block is provided with a limiting part extending towards the center of the cavity, so that the limiting part abuts against the stopping part to prevent the pressing unit from being separated from the cavity.

4. A quick cutting rule according to claim 1, wherein: the rubber ruler is characterized in that track grooves are formed in the top of the rubber ruler body along two sides of the length direction, and inserting plates inserted into the track grooves extend outwards from the bottom of the moving block, so that the moving block can move back and forth along the track grooves of the rubber ruler body.

5. A quick cutting rule according to claim 1, wherein: the top of the pressing unit is provided with a button, and the top surface of the button is provided with anti-skidding convex points or anti-skidding stripes.

6. A quick cutting rule according to claim 1, wherein: the supporting seat is fixedly connected with the rubber ruler body or integrally formed.

7. A quick cutting rule according to claim 1, wherein: the blade is detachably connected with the bottom of the pressing unit.

8. A quick cutting rule according to claim 1, wherein: the rubber ruler body is marked with length scales.

9. A quick cutting rule according to claim 8, wherein: the length of the graduated scale of the rubber ruler body is 10 cm.

10. A quick cutting rule according to claim 1, wherein: the height of the supporting seat is 1 cm.

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