CN212504855U - Stem cell separation device - Google Patents
Stem cell separation device Download PDFInfo
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- CN212504855U CN212504855U CN201921970226.9U CN201921970226U CN212504855U CN 212504855 U CN212504855 U CN 212504855U CN 201921970226 U CN201921970226 U CN 201921970226U CN 212504855 U CN212504855 U CN 212504855U
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Abstract
The invention discloses a stem cell separation device, and relates to the field of stem cells. The method comprises the following steps: shell, shell top one side is provided with the discharge gate, discharge gate top level is provided with the stem cell storage cylinder, stem cell storage cylinder top is provided with the feed inlet, the feed inlet top is provided with the rubber buffer, stem cell storage cylinder inner chamber is provided with the piston, the outside of piston is provided with the piston rod, the outside of piston rod is provided with pushes away the handle, the top center department of shell is provided with the motor, the motor bottom is provided with the axis of rotation, the axis of rotation extends to the inner chamber of shell always, the spiral stirring leaf is around being provided with in the axis of rotation, the axis of rotation below is provided with filtration membrane one, filtration membrane one bottom is provided with the spray tank, the spray tank bottom. Has the advantages that: the liquid medicine is uniformly sprayed out through the spray header and the spray holes below the spray header and is uniformly mixed with the substances below the spray header, so that the separation efficiency is improved.
Description
Technical Field
The utility model relates to a stem cell field particularly, relates to a stem cell separator.
Background
Stem cells are primitive cells having self-renewal and multipotential differentiation potential, which are cells of origin of the body and can differentiate into one or more cells constituting human tissues or organs under specific conditions. For mammals, stem cells fall into two broad categories: embryonic stem cells and adult stem cells. The stem cell can repair damaged tissue cells, replace the functions of the damaged cells through division and differentiation, stimulate the regeneration function of the body cells through secreting protein factors, can be used for treating various diseases including blood immune system diseases, nervous system diseases, cardiovascular and cerebrovascular diseases, tumors and the like, and is an important resource foundation of regenerative medicine.
At present, the shearing process of partial stem cells is carried out manually, a large amount of cells are damaged, the survival rate of the stem cells is reduced, and meanwhile, blood is wasted. Most traditional separator procedures are complicated, can't accomplish fine separation and recovery to stem cells, and the separation effect is relatively poor, and the device also can't accomplish stage nature and orderliness simultaneously, and taking out and clearing up of material after the separation is more difficult, has influenced the effective utilization to stem cells greatly.
An effective solution to the problems in the related art has not been proposed yet.
Disclosure of Invention
To solve the problems in the related art, the present invention provides a stem cell separation device to overcome the above technical problems in the related art.
The technical scheme of the utility model is realized like this:
a stem cell separation device comprises a shell, wherein a discharge port is formed in one side of the top end of the shell, a stem cell storage cylinder is horizontally arranged at the top end of the discharge port, a feed inlet is formed in the top end of the stem cell storage cylinder, a rubber plug is arranged at the top end of the feed inlet, a piston is arranged in an inner cavity of the stem cell storage cylinder, a piston rod is arranged on the outer side of the piston, a push handle is arranged on the outer side of the piston rod, a motor is arranged at the center of the top end of the shell, a rotating shaft is arranged at the bottom end of the motor and extends to the inner cavity of the shell all the time, spiral stirring blades are arranged on the rotating shaft in a surrounding mode, a first filtering membrane is arranged below the rotating shaft, a first filtering membrane is arranged at the bottom end of the first filtering membrane, the filtering membrane II is provided with a liquid outlet below, a liquid collecting device is arranged below the liquid outlet, one side of the shell is provided with a first pipeline between the rotating shaft and the filtering membrane I, the same side of the shell is provided with a second pipeline between the spray head and the filtering membrane II, a first collecting box is arranged outside the first pipeline, and a second collecting box is arranged at the bottom end of the first collecting box and connected with the second pipeline.
Furthermore, the first pipeline and the second pipeline are provided with fans.
Furthermore, control valves are arranged on the first pipeline and the second pipeline.
Furthermore, one section of the discharge hole is provided with a one-way valve.
Further, the shell is of a double-layer hollow structure.
The beneficial effects of this utility do: the use of the stem cells is ordered by utilizing the one-way valve; the crushed cells are filtered by a filter screen to separate suspended matters from liquid, and the cells are convenient to clean; the liquid medicine is uniformly sprayed out through the spray header and the spray holes below the spray header and is uniformly mixed with the substances below the spray header, so that the separation efficiency is improved; the fan and the collecting box can effectively collect separated substances, and meanwhile, required substances can be conveniently collected and taken out.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without inventive efforts.
Fig. 1 is a perspective view according to an embodiment of the present invention;
FIG. 2 is a cross-sectional view according to an embodiment of the present invention;
fig. 3 is a diagram of an explosive device according to an embodiment of the present invention;
fig. 4 is a partial view of a feeding portion according to an embodiment of the present invention.
In the figure:
1. a housing; 2. a discharge port; 3. a one-way valve; 4. a stem cell storage cylinder; 5. a feed inlet; 6. a rubber plug; 7. a piston; 8. a piston rod; 9. a push handle; 10. an electric motor; 11. a rotating shaft; 12. a spiral stirring blade; 13. a first filtering membrane; 14. a liquid medicine box; 15. a shower head; 16. spraying holes; 17. a second filtering membrane; 18. a liquid outlet; 19. a liquid collection device; 20. a first pipeline; 21. a second pipeline; 22. A fan; 23. a first collecting box; 24. and a second collecting box.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application belong to the scope of protection of the present application.
According to an embodiment of the present invention, there is provided a stem cell separation device.
As shown in fig. 1-4, a stem cell separation device according to an embodiment of the present invention includes a housing 1, and is characterized in that a discharge port 2 is disposed on one side of a top end of the housing 1, a stem cell storage cylinder 4 is horizontally disposed on a top end of the discharge port 2, a feed port 5 is disposed on a top end of the stem cell storage cylinder 4, a rubber plug 6 is disposed on a top end of the feed port 5, a piston 7 is disposed in an inner cavity of the stem cell storage cylinder 4, a piston rod 8 is disposed on an outer side of the piston 7, a push handle 9 is disposed on an outer side of the piston rod 8, a motor 10 is disposed at a center of a top end of the housing 1, a rotation shaft 11 is disposed at a bottom end of the motor, the rotation shaft 11 extends to the inner cavity of the housing 1, a spiral stirring blade 12 is disposed around the rotation shaft 11, a first filtering membrane, 14 bottoms of spray tank are provided with shower head 15, there are a plurality of spray holes 16 in the 15 bottom surfaces of shower head, shower head 15 below is provided with filtration membrane two 17, the below of filtration membrane two 17 is provided with liquid outlet 18, liquid outlet 18 below is provided with liquid collection device 19, one side of shell 1 just the axis of rotation 11 with be provided with pipeline one 20 between the filtration membrane one 13, shell 1 with one side just be in the shower head 15 with be provided with pipeline two 21 between the filtration membrane two 17, the pipeline one 20 outside is provided with collecting box one 23, collecting box one 23 bottom just what pipeline two 21 connect is provided with collecting box two 24.
In one embodiment, the first pipe 20 and the second pipe 21 are provided with the fan 22, so that when the separated substances do not pass through the first filtering membrane 13 and the second filtering membrane 17, the separated substances can be quickly sucked into the first pipe 20 and the second pipe 21 by the fan 22 and then quickly collected into the first collecting box 23 and the second collecting box 24, and the separation and recovery effects are achieved. In addition, in a specific application, it should be noted that the blower 22 should be turned on after one separation, otherwise, substances which are not completely separated are sucked by mistake, and the effect cannot be achieved.
In one embodiment, the first pipeline 20 and the second pipeline 21 are provided with control valves, so that when the fan 22 is not operated, other substances are prevented from entering the first pipeline 20 and the second pipeline 21, and the separation effect is effectively achieved. In addition, in a specific application, whether the control valve is closed or not should be checked in advance before the device is used.
In one embodiment, one section of the outlet 2 is provided with a one-way valve 3, so that when stem cells enter the stem cell storage cylinder 4, the stem cells can only enter the device through the outlet 2, and cannot flow back to the stem cell storage cylinder 4 when the piston 7 is pulled outwards, thereby further ordering the process. In addition, in the specific application, it should be noted that the check valve 3 enters the discharge port 2 from the stem cell storage cylinder 4, so as to avoid reverse installation.
In one embodiment, the housing 1 has a double-layered hollow structure, thereby effectively insulating heat and preventing external factors from affecting the inside of the device, so that the device can be effectively operated, and the activity of cells can be maintained without a large temperature change. In addition, in specific applications, the regular inspection of the casing 1 should be taken care of to prevent the occurrence of damages and the like, which would cause serious damage to the heat insulation effect of the device.
In summary, according to the above technical solution of the present invention, a user first closes the control valve, opens the rubber plug 6, puts stem cells into the stem cell storage cylinder 4 through the feed inlet 5, drives the piston rod 8 by the push handle 9, so that the piston 7 compresses the stem cells in the stem cell storage cylinder 4, and slowly puts them into the housing 1 through the one-way valve 3 and the discharge outlet 2, at this time, opens the switch, the motor 10 starts to operate, the rotating shaft 11 starts to rotate, the spiral stirring blade 12 starts to repeatedly cut the stem cells, the separated substances with too large diameter are separated by the first filtering membrane 13, the rest of the substances enter the next part through the first filtering membrane 13, at this time, the liquid medicine stored in the liquid medicine tank 14 is uniformly sprinkled on the substances through the sprinkling head 15 by the sprinkling holes 16, so that the stem cells are fully immersed and separated, and then effectively separated by the second filtering membrane 17, so that the liquid enters the liquid collecting device through the liquid outlet 18, at the moment, the control valve is opened, the fan 22 is opened, and the substances remained on the first filtering membrane 13 and the second filtering membrane 17 enter the first collecting box 23 and the second collecting box 24 through the first pipeline 20 and the second pipeline 21, so that the separation effect is achieved.
Has the advantages that: 1. the one-way valve 3 is utilized to ensure that the stem cells are used more orderly; 2. the crushed cells are filtered by the first filter screen 13 and the second filter screen 17, so that suspended matters and liquid are separated, and the cells are convenient to clean; 3. the liquid medicine is uniformly sprayed out through the spray header 15 and the spray holes 16 below, and is uniformly mixed with the substances below, so that the separation efficiency is improved; 4. the fan 22, the first collection box 23 and the second collection box 24 can effectively collect separated substances, and meanwhile, required substances can be conveniently collected and taken out.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the present invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (5)
1. A stem cell separation device, comprising: shell (1), its characterized in that, shell (1) top one side is provided with discharge gate (2), discharge gate (2) top level is provided with stem cell storage cylinder (4), stem cell storage cylinder (4) top is provided with feed inlet (5), feed inlet (5) top is provided with rubber buffer (6), stem cell storage cylinder (4) inner chamber is provided with piston (7), the outside of piston (7) is provided with piston rod (8), the outside of piston rod (8) is provided with pushes away handle (9), the top center department of shell (1) is provided with motor (10), the motor bottom is provided with axis of rotation (11), axis of rotation (11) extend to the inner chamber of shell (1) always, it is provided with spiral stirring leaf (12) to encircle in axis of rotation (11), axis of rotation (11) below is provided with filtration membrane (13), a liquid medicine box (14) is arranged at the bottom end of the first filtering membrane (13), a spray header (15) is arranged at the bottom end of the liquid medicine box (14), a plurality of spray holes (16) are arranged on the bottom surface of the spray head (15), a second filtering membrane (17) is arranged below the spray head (15), a liquid outlet (18) is arranged below the second filtering membrane (17), a liquid collecting device (19) is arranged below the liquid outlet (18), a first pipeline (20) is arranged on one side of the shell (1) and between the rotating shaft (11) and the first filtering membrane (13), a second pipeline (21) is arranged on the same side of the outer shell (1) and between the spray header (15) and the second filtering membrane (17), a first collecting box (23) is arranged on the outer side of the first pipeline (20), and a second collecting box (24) is arranged at the bottom end of the first collecting box (23) and connected with the second pipeline (21).
2. The stem cell separation device according to claim 1, wherein a blower (22) is provided on the first pipe (20) and the second pipe (21).
3. The stem cell separation device according to claim 1, wherein the first pipe (20) and the second pipe (21) are provided with control valves.
4. A stem cell separation device according to claim 1, wherein a section of the outlet (2) is provided with a one-way valve (3).
5. A stem cell separation device according to claim 1, wherein the housing (1) has a double-layer hollow structure.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201921970226.9U CN212504855U (en) | 2019-11-15 | 2019-11-15 | Stem cell separation device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201921970226.9U CN212504855U (en) | 2019-11-15 | 2019-11-15 | Stem cell separation device |
Publications (1)
Publication Number | Publication Date |
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CN212504855U true CN212504855U (en) | 2021-02-09 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201921970226.9U Active CN212504855U (en) | 2019-11-15 | 2019-11-15 | Stem cell separation device |
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CN (1) | CN212504855U (en) |
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2019
- 2019-11-15 CN CN201921970226.9U patent/CN212504855U/en active Active
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