CN212433186U - Furacilin, furazolidone and chloramphenicol immunofluorescence chromatography triple rapid detection card - Google Patents
Furacilin, furazolidone and chloramphenicol immunofluorescence chromatography triple rapid detection card Download PDFInfo
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- CN212433186U CN212433186U CN202023155601.XU CN202023155601U CN212433186U CN 212433186 U CN212433186 U CN 212433186U CN 202023155601 U CN202023155601 U CN 202023155601U CN 212433186 U CN212433186 U CN 212433186U
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Abstract
The utility model belongs to the technical field of detect, it discloses a furacilin, furazolidone and chloramphenicol immunofluorescence chromatography trigeminy short-term test card, include box body down, be equipped with the furacilin detecting element that three platoon was arranged down on the box body, furaciidone detecting element, chloramphenicol detecting element have realized the detection to furacilin, furacizolidone and chloramphenicol effectively, and its sensitivity is high, and the testing result is reliable, and is more directly perceived, quick to the observation of furacilin, furacizolinone and chloramphenicol testing result.
Description
Technical Field
The utility model relates to a detect technical field, specifically be a furacilin, furazolidone and chloramphenicol immunofluorescence chromatography trigeminy short-term test card.
Background
In the prior art, veterinary drugs are used in the breeding industry, and the veterinary drugs which are easy to cause the residual quantity of the veterinary drugs to exceed the standard in animal-derived foods mainly comprise antibiotics, sulfonamides, furans, antiparasitics and hormones drugs, can be transmitted to human beings through a food chain, can cause various diseases after being taken for a long time, and have side effects of carcinogenesis, teratogenesis and the like on the human bodies.
Wherein, furans such as furacilin and furazolidone are commonly used in the feed of pigs or chickens for preventing diseases, and the furans have zero residue in animal-derived food, namely cannot be detected and are banned veterinary drugs for food animals in China;
whereas chloramphenicol, among the antibiotics, can cause severe aplastic anemia (granulocytes or whole blood cells) and occurs regardless of dosage and frequency of use, humans are more sensitive to chloramphenicol and more toxic than animals;
therefore, the residue detection of furacilin, furazolidone and chloramphenicol is particularly important.
The utility model discloses a patent publication No. CN206945709U, it discloses a aquatic animal remedy residue allies oneself with quick detection device more, including detecting the card, detect the card and include the bottom plate, locate the water absorption pad of bottom plate, and locate the liquid-proof pad of water absorption pad, the protruding bulge that is equipped with of one end of water absorption pad forms the recess, the liquid-proof pad is held in the recess at least partially, detect the card and still include along its first end to the direction of second end paste in proper order in the sample pad and the colloidal gold combination pad of liquid-proof pad, detect the card still include locate the nitrocellulose membrane of liquid-proof pad and bulge, overlap the blotter of locating the sample pad; the nitrocellulose membrane is sequentially provided with a first detection line, a second detection line, a third detection line, a fourth detection line, a fifth detection line, a sixth detection line, a seventh detection line, an eighth detection line and a quality control line along the direction from the first end to the second end, and the quality control line is coated with goat anti-rabbit IgG.
In foretell a scheme of aquatic products animal remedy remains and allies oneself with quick detection device more, its setting through first detection line, second detection line, third detection line, fourth detection line, fifth detection line, sixth detection line, seventh detection line, eighth detection line comes to remain to detect aquatic products animal remedy.
The problem that the prior art exists is that a plurality of detection lines detect different parameters to be detected simultaneously, the detection line and the parameters to be detected need to be compared one by one to obtain the specific detection result of which parameter, the observation of the detection results of furacilin, furazolidone and chloramphenicol is not intuitive enough, and whether furacilin, furazolidone and chloramphenicol still remain or not can not be judged quickly.
Therefore, the technical problem to be solved by the application is as follows: how to provide a rapid detection card for detecting furacilin, furazolidone and chloramphenicol, which can more intuitively and rapidly observe the detection result.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a furacilin, furazolidone and chloramphenicol immunofluorescence chromatography trigeminy short-term test card, its sensitivity is high, and the testing result is reliable, and is more directly perceived, quick to the observation of furacilin, furazolidone and chloramphenicol testing result.
In order to achieve the above object, the utility model provides a following technical scheme:
an immunofluorescence chromatography triple rapid detection card for furacilin, furazolidone and chloramphenicol comprises a lower box body, wherein the lower box body is provided with three furacilin detection units, furazolidone detection units and chloramphenicol detection units which are arranged in parallel,
the nitrofurazone detection unit comprises a first bottom plate, a first sample pad, a first combination pad, a first nitrocellulose membrane and a first water absorption pad are sequentially connected to the first bottom plate from left to right, and a mixture of a nitrofurazone monoclonal antibody marked by fluorescent microspheres and goat anti-chicken IgY is loaded on the first combination pad; the first nitrocellulose membrane is provided with a first quality control line and a first detection line in parallel, the first quality control line is coated with chicken IgY, and the first detection line is coated with furacilin-BSA;
the furazolidone detection unit comprises a second bottom plate, a second sample pad, a second combination pad, a second nitrocellulose membrane and a second water absorption pad are sequentially connected to the second bottom plate from left to right, a mixture of a furazolidone monoclonal antibody marked by fluorescent microspheres and goat anti chicken IgY is loaded on the second combination pad, a second quality control line and a second detection line are arranged in parallel on the second nitrocellulose membrane, the chicken IgY is coated on the second quality control line, and furazolidone-OVA is coated on the second detection line;
the chloramphenicol detection unit comprises a third bottom plate, a third sample pad, a third combination pad, a third nitrocellulose membrane and a third water absorption pad are sequentially connected to the third bottom plate from left to right, a mixture of a chloramphenicol monoclonal antibody marked by fluorescent microspheres and goat anti-chicken IgY is loaded on the third combination pad, a third quality control line and a third detection line are arranged in parallel on the third nitrocellulose membrane, the chicken IgY is coated on the third quality control line, and chloramphenicol-BSA is coated on the third detection line.
In foretell furacilin, furazolidone and chloramphenicol immunofluorescence chromatography trigeminy short-term test card, still include the lid of connecting box body under can dismantling, box body and lid cooperation form the installation cavity down, and three detecting element all sets up in the installation cavity.
In the furacilin, furazolidone and chloramphenicol three-way rapid detection card for immunofluorescence chromatography, the cover body is provided with sample adding holes which are in one-to-one correspondence with the sample pads in each detection unit and observation windows which are in one-to-one correspondence with the nitrocellulose membranes in each detection unit.
In the furacilin, furazolidone and chloramphenicol immunofluorescence chromatography triple rapid detection card, the sample addition hole and the observation window are conical holes.
In the furacilin, furazolidone and chloramphenicol immunofluorescence chromatography triple rapid detection card, the cover body is also provided with air holes which are in one-to-one correspondence with the water absorption pads in each detection unit.
In the furacilin, furazolidone and chloramphenicol immunofluorescence chromatography triple rapid detection card, the inner side of the lower box body is provided with an installation mechanism for installing three detection units, and one detection unit is fixed in each installation mechanism.
In the furacilin, furazolidone and chloramphenicol immunofluorescence chromatography triple rapid detection card, the mounting mechanism comprises a U-shaped mounting block and a first limiting plate, the U-shaped mounting block is arranged on the lower box body, and the first limiting plate is arranged corresponding to the open end of the U-shaped mounting block.
In the furacilin, furazolidone and chloramphenicol immunofluorescence chromatography triple rapid detection card, the mounting mechanism further comprises a second limiting plate and a third limiting plate which are arranged between the U-shaped mounting block and the first limiting plate, and the second limiting plate and the third limiting plate are equally divided and arranged on two sides of the detection unit and are two.
In the furacilin, furazolidone and chloramphenicol three-way rapid detection card for immunofluorescence chromatography, elastic salient points are arranged on the inner wall opposite to the U-shaped mounting block, the side walls opposite to the two second limiting plates and the side walls opposite to the two third limiting plates.
In the above furacilin, furazolidone and chloramphenicol immunofluorescence chromatography triple rapid detection card, the detection card further comprises a positioning part, wherein the positioning part comprises first concave parts which are symmetrically arranged on two side edges of the lower box body and second concave parts which are arranged on the side edge of the cover body and are matched with the first concave parts.
Compared with the prior art, the beneficial effects of the utility model are that:
in the utility model, the detection of nitrofurazone, furazolidone and chloramphenicol is realized by arranging 3 detection units on the lower box body, and compared with the prior art, the detection device can more intuitively and quickly observe the corresponding detection results;
meanwhile, only one parameter is detected by a single detection unit, so that the accuracy of a detection result is ensured, and the influence of other parameters on the single detection result is reduced.
Drawings
Fig. 1 is a structure diagram of the cooperation of the lower box body and each detection unit in embodiment 1 of the present invention;
FIG. 2 is a structural diagram of a furacilin detection unit of example 1 of the present invention;
fig. 3 is a perspective view of embodiment 1 of the present invention;
fig. 4 is a bottom view of the cover according to embodiment 1 of the present invention;
fig. 5 is a structural view of a lower case according to embodiment 1 of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example 1
Referring to fig. 1 to 5, a triple rapid detection card for immunofluorescence chromatography of furacilin, furazolidone and chloramphenicol comprises a lower box body 1, wherein the lower box body 1 is provided with three furacilin detection units a, furazolidone detection units B and chloramphenicol detection units C which are arranged in parallel,
the furacilin detection unit A comprises a first base plate 11, wherein a first sample pad 12, a first combination pad 13, a first nitrocellulose membrane 14 and a first water absorption pad 15 are sequentially connected to the first base plate 11 from left to right, and a mixture of a furacilin monoclonal antibody marked by fluorescent microspheres and goat anti-chicken IgY is loaded on the first combination pad 13; the first nitrocellulose membrane 14 is provided with a first quality control line C1 and a first detection line T1 in parallel, the first quality control line C1 is coated with chicken IgY, and the first detection line T1 is coated with furacilin-BSA;
the furazolidone detection unit B comprises a second bottom plate (not shown in the figure), a second sample pad 21, a second combination pad 22, a second nitrocellulose membrane 23 and a second water absorption pad 24 are sequentially connected to the second bottom plate from left to right, a mixture of a furazolidone monoclonal antibody marked by fluorescent microspheres and goat anti-chicken IgY is loaded on the second combination pad 22, a second quality control line C2 and a second detection line T2 are arranged in parallel on the second nitrocellulose membrane 23, the chicken IgY is coated on the second quality control line C2, and the furazolidone-OVA is coated on the second detection line T2;
the chloramphenicol detecting unit C comprises a third base plate (not shown in the figure), a third sample pad 31, a third binding pad 32, a third nitrocellulose membrane 33 and a third absorbent pad 34 are sequentially connected to the third base plate from left to right, a mixture of a chloramphenicol monoclonal antibody labeled with fluorescent microspheres and goat anti-chicken IgY is loaded on the third binding pad 32, a third quality control line C3 and a third detection line T3 are arranged in parallel on the third nitrocellulose membrane 33, the chicken IgY is coated on the third quality control line C3, and chloramphenicol-BSA is coated on the third detection line T3.
In practical application, the detection of nitrofurazone, furazolidone and chloramphenicol is realized by arranging the three detection units on the lower box body 1, and compared with the prior art, the detection device can more intuitively and quickly observe corresponding detection results;
meanwhile, only one parameter is detected by a single detection unit, so that the accuracy of a detection result is ensured, and the influence of other parameters on the single detection result is reduced;
referring to fig. 2, the second bottom plate, the second sample pad 21, the second conjugate pad 22, the second nitrocellulose membrane 23, and the second absorbent pad 24 of the furazolidone detecting unit B have the same structure as that of the furacilin detecting unit a; the structures of the third bottom plate, the third sample pad 31, the third combination pad 32, the third nitrocellulose membrane 33 and the third absorbent pad 34 of the chloramphenicol detecting unit C are the same as those of the furacilin detecting unit a.
In this embodiment, referring to fig. 3, the detection device further includes a cover 4 detachably connected to the lower box body 1, the lower box body 1 and the cover 4 cooperate to form a mounting cavity, and the three detection units are all disposed in the mounting cavity; the cover body 4 is provided with sample adding holes 41 corresponding to the sample pads in the detection units one by one, and observation windows 42 corresponding to the nitrocellulose membranes in the detection units one by one.
When the detection of the sample is realized, the sample is added into the sample adding hole 41, and the sample reacts at the position of the nitrocellulose membrane; the detection result of furacilin is obtained by comparing a first detection line T1 with a first quality control line C1, the detection result of furazolidone is obtained by comparing a second detection line T2 with a second quality control line C2, and the detection result of chloramphenicol is obtained by comparing a third detection line T3 with a third quality control line C3.
Preferably, the sampling hole 41 and the observation window 42 are both tapered holes.
As a preferred specific implementation of this embodiment, the sampling hole 41 and the observation window 42 may be tapered holes spatially complementary to a circular truncated cone, or may also be tapered holes spatially complementary to a four-cone frustum, which is not limited in this embodiment, and as a specific implementation of this embodiment, the sampling hole 41 and the observation window 42 are tapered holes spatially complementary to a four-cone frustum;
specifically, the sample adding hole 41 which is a tapered hole complementary to the four-frustum in shape in space is beneficial to adding a sample and plays a role in flow guiding; the observation window 42 is provided with a transparent observation plate, and the observation window 42 with the shape of a conical hole which is complementary with the four frustum in space facilitates the installation of the observation plate.
Preferably, the cover 4 is further provided with air holes 43 corresponding to the absorbent pads in the respective detection units one by one. The air in the installation cavity is timely discharged, the detection process is further accelerated, the detection efficiency is improved, and in the embodiment, the air holes 43 are formed by uniformly arranging a plurality of small holes.
In the present embodiment, referring to fig. 5, the lower case 1 is provided inside with mounting mechanisms 5 for mounting three detecting units, and one detecting unit is fixed inside each mounting mechanism 5.
Preferably, the mounting mechanism 5 comprises a U-shaped mounting block 51 and a first limiting plate 52 arranged on the lower box body 1, the first limiting plate 52 is arranged corresponding to the open end of the U-shaped mounting block 51, and the distance between the U-shaped mounting block 51 and the first limiting plate 52 is matched with the length of the first bottom plate 11, the second bottom plate and the third bottom plate, so that the first bottom plate 11, the second bottom plate and the third bottom plate can be conveniently mounted and dismounted;
of course, the mounting mechanism 5 may also be mounting grooves adapted to the first bottom plate 11, the second bottom plate, and the third bottom plate, which is not limited in this embodiment.
In order to improve the stability of the installation of the detection unit, more preferably, the installation mechanism 5 further includes a second limiting plate 53 and a third limiting plate 54 disposed between the U-shaped installation block 51 and the first limiting plate 52, where the second limiting plate 53 and the third limiting plate 54 are respectively disposed on two sides of the detection unit, and both sides of the detection unit are limited in the length direction.
More preferably, the opposite inner walls of the U-shaped mounting block 51, the opposite side walls of the two second limiting plates 53, and the opposite side walls of the two third limiting plates 54 are all provided with elastic bumps 6, which play a role in fastening the detection unit, and further improve the stability of the detection unit mounting.
In this embodiment, the detachable connection between the lower case 1 and the cover 4 may be realized by a bolt connection, and as a specific implementation of this embodiment, the detachable connection between the lower case 1 and the cover 4 is realized by a way of matching the plug-in body 8 with the plug-in hole 9, which has a simple structure and is convenient to install and disassemble;
in this embodiment, a plurality of columns are uniformly arranged on the lower box body 1, the columns are provided with insertion holes 9, the cover body 4 is provided with insertion bodies 8 matched with the insertion holes 9, of course, the insertion holes 9 and the insertion bodies 8 may also be arranged on the lower box body 1 and the cover body 4 in an intersecting manner, which is not limited in this embodiment;
as a specific implementation of this embodiment, referring to fig. 4 to 5, along the length direction of the lower box body 1, a first inserting hole 81, a second inserting hole 82, a third inserting hole 83 and a first inserting body 91 are respectively disposed on two sides of the lower box body 1, and the first inserting hole 81, the second inserting hole 82, the third inserting hole 83 and the first inserting body 91 are uniformly disposed;
the cover 4 is provided with a second plug-in body 92 adapted to the first plug-in hole 81, a third plug-in body 93 adapted to the second plug-in hole 82, a fourth plug-in body 94 adapted to the third plug-in hole 83, and a fourth plug-in hole 84 adapted to the first plug-in body 91, so that the stability of the installation of the lower box body 1 and the cover 4 is effectively improved.
Be equipped with first annular surrounding edge 10 down on the contact surface of box body 1 and lid 4, the medial surface of first annular surrounding edge 10 and the inner wall parallel and level of box body 1 down, be equipped with the second annular surrounding edge 40 with first annular surrounding edge 10 on the contact surface of lid 4 and lower box body 1, the lateral surface of second annular surrounding edge 40 and the outer wall parallel and level of lid 4, the lateral surface of first annular surrounding edge 10 and the inside wall cooperation of second annular surrounding edge 40 further improve the stability of box body 1 and lid 4 installation down, and it also can play the positioning action of both installations.
Preferably, the box body further comprises a positioning part 7, wherein the positioning part 7 comprises first concave parts 71 which are symmetrically arranged on two side edges of the lower box body 1 and second concave parts 72 which are arranged on the side edges of the cover body 4 and matched with the first concave parts 71, so that an operator can conveniently install the lower box body 1 and the cover body 4;
specifically, the number of the first concave portions 71 and the second concave portions 72 may be 1, 2 or more, which is not limited in the present embodiment; as a specific implementation of this embodiment, the first recesses 71 are 2 recesses symmetrically disposed on both sides of the lower case 1 in the longitudinal direction, and the second recesses 72 are 2 recesses symmetrically disposed on both sides of the cover 4 in the longitudinal direction.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (10)
1. A furacilin, furazolidone and chloramphenicol immunofluorescence chromatography triple rapid detection card comprises a lower box body (1), and is characterized in that the lower box body (1) is provided with three furacilin detection units (A), furazolidone detection units (B) and chloramphenicol detection units (C) which are arranged in parallel;
the furacilin detection unit (A) comprises a first base plate (11), a first sample pad (12), a first combination pad (13), a first nitrocellulose membrane (14) and a first water absorption pad (15) are sequentially connected to the first base plate (11) from left to right, and a mixture of a furacilin monoclonal antibody marked by fluorescent microspheres and goat anti-chicken IgY is loaded on the first combination pad (13); the first nitrocellulose membrane (14) is provided with a first quality control line (C1) and a first detection line (T1) in parallel, the first quality control line (C1) is coated with chicken IgY, and the first detection line (T1) is coated with furacilin-BSA;
the furazolidone detection unit (B) comprises a second bottom plate, a second sample pad (21), a second combination pad (22), a second nitrocellulose membrane (23) and a second water absorption pad (24) are sequentially connected to the second bottom plate from left to right, a mixture of a furazolidone monoclonal antibody marked by fluorescent microspheres and goat anti-chicken IgY is loaded on the second combination pad (22), a second quality control line (C2) and a second detection line (T2) are arranged in parallel on the second nitrocellulose membrane (23), the chicken IgY is coated on the second quality control line (C2), and furazolidone-OVA is coated on the second detection line (T2);
the chloramphenicol detection unit (C) comprises a third bottom plate, a third sample pad (31), a third binding pad (32), a third nitrocellulose membrane (33) and a third water absorption pad (34) are sequentially connected to the third bottom plate from left to right, a mixture of a chloramphenicol monoclonal antibody labeled by fluorescent microspheres and goat anti-chicken IgY is loaded on the third binding pad (32), a third quality control line (C3) and a third detection line (T3) are arranged in parallel on the third nitrocellulose membrane (33), the chicken IgY is coated on the third quality control line (C3), and chloramphenicol-BSA is coated on the third detection line (T3).
2. The furacilin, furazolidone and chloramphenicol immune fluorescence chromatography triple rapid detection card as claimed in claim 1, which is characterized by further comprising a cover body (4) detachably connected with the lower box body (1), wherein the lower box body (1) and the cover body (4) are matched to form an installation cavity, and the three detection units are all arranged in the installation cavity.
3. The furacilin, furazolidone and chloramphenicol three-linked rapid detection card for immunofluorescence chromatography according to claim 2, wherein the cover body (4) is provided with sample adding holes (41) corresponding to the sample pads in each detection unit one by one, and observation windows (42) corresponding to the nitrocellulose membranes in each detection unit one by one.
4. The furacilin, furazolidone and chloramphenicol immune fluorescent chromatography triple rapid detection card as claimed in claim 3, characterized in that the sample adding hole (41) and the observation window (42) are conical holes.
5. The furacilin, furazolidone and chloramphenicol three-linked rapid detection card for immunofluorescence chromatography according to claim 2, wherein the cover body (4) is further provided with air holes (43) corresponding to the water absorption pads in the detection units one by one.
6. The furacilin, furazolidone and chloramphenicol three-way immunofluorescence chromatography rapid detection card as claimed in claim 2, wherein the inner side of the lower box body (1) is provided with an installation mechanism (5) for installing three detection units, and one detection unit is fixed in each installation mechanism (5).
7. The furacilin, furazolidone and chloramphenicol three-component rapid detection card for immunofluorescence chromatography according to claim 6, wherein the installation mechanism (5) comprises a U-shaped installation block (51) arranged on the lower box body (1) and a first limit plate (52), and the first limit plate (52) is arranged corresponding to the open end of the U-shaped installation block (51).
8. The furacilin, furazolidone and chloramphenicol three-way rapid detection card for immunofluorescence chromatography according to claim 7, wherein the mounting mechanism (5) further comprises a second limiting plate (53) and a third limiting plate (54) which are arranged between the U-shaped mounting block (51) and the first limiting plate (52), the second limiting plate (53) and the third limiting plate (54) are respectively arranged on two sides of the detection unit, and the number of the second limiting plate and the third limiting plate is two.
9. The furacilin, furazolidone and chloramphenicol three-way rapid detection card for immunofluorescence chromatography according to claim 7, wherein the opposite inner walls of the U-shaped mounting block (51), the opposite side walls of the two second limiting plates (53) and the opposite side walls of the two third limiting plates (54) are provided with elastic bumps (6).
10. The furacilin, furazolidone and chloramphenicol immune fluorescence chromatography triple rapid detection card as claimed in claim 2, which is characterized by further comprising a positioning part (7), wherein the positioning part (7) comprises a first concave part (71) which is arranged on two sides of the lower box body (1) symmetrically, and a second concave part (72) which is arranged on the side edge of the cover body and is matched with the first concave part (71).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023155601.XU CN212433186U (en) | 2020-12-24 | 2020-12-24 | Furacilin, furazolidone and chloramphenicol immunofluorescence chromatography triple rapid detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023155601.XU CN212433186U (en) | 2020-12-24 | 2020-12-24 | Furacilin, furazolidone and chloramphenicol immunofluorescence chromatography triple rapid detection card |
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| CN212433186U true CN212433186U (en) | 2021-01-29 |
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| CN202023155601.XU Active CN212433186U (en) | 2020-12-24 | 2020-12-24 | Furacilin, furazolidone and chloramphenicol immunofluorescence chromatography triple rapid detection card |
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| CN (1) | CN212433186U (en) |
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