CN212388046U

CN212388046U - Adipose-derived stem cell exosome culture device

Info

Publication number: CN212388046U
Application number: CN202020783834.5U
Authority: CN
Inventors: 田剑; 傅亚宁; 李泽辉
Original assignee: Tianjin City Jinhua Biotechnology Co ltd
Current assignee: Tianjin City Jinhua Biotechnology Co ltd
Priority date: 2020-05-13
Filing date: 2020-05-13
Publication date: 2021-01-22
Anticipated expiration: 2030-05-13

Abstract

The utility model discloses an adipose-derived stem cell exosome culture device belongs to the cell culture field, including the incubator, the thermocouple is installed in incubator inside wall left side, the heater strip is installed on the inside wall right side of incubator, the input of heater strip and the output electric connection of thermocouple, temperature controller is installed to the lateral wall of incubator, the fixed block is installed at the inside wall top of incubator, the spring is installed to the lateral wall of fixed block, the spring has two sets of the symmetry of being to install the lateral wall at the fixed block, U type piece is installed to the other end of spring. The utility model discloses a thereby temperature controller, thermocouple and heater strip mutually support can effectual control incubator in to can improve adipose stem cell's quality, rethread CCD camera mutually supports with image processor, is fit for being extensively promoted and used.

Description

Adipose-derived stem cell exosome culture device

Technical Field

The utility model relates to a cell culture technical field especially relates to an adipose-derived stem cell exosome culture device.

Background

The adipose-derived stem cells are stem cells with the potential of multi-directional differentiation separated from adipose tissues in recent years, mainly restore the repairing function of tissue cells, promote the regeneration of cells, restore the young face, fully improve the body functions, effectively improve diseases such as sub-health, premature senility and the like, and really and effectively resist aging from inside to outside.

Stem cells are multipotent cells with self-renewal ability, which can be differentiated into various functional cells or tissues and organs under certain conditions, and are called universal cells in the medical field, the stem cells refer to cells which are not differentiated, exist in early embryos, placentas, accessories thereof, bone marrow, peripheral blood and adult tissues, and can be cultured into more than two hundred human cells, tissues and organs such as muscles, bones, nerves and the like.

Patent number CN discloses a novel adipose-derived stem cell exosome culture device, the on-line screen storage device comprises a base, the upper surface of base is seted up flutedly, the fixed plate has been placed to the inside of recess, the case is placed to the last fixed surface of fixed plate is connected with, the culture dish has been placed to the inside of placing the case, the last fixed surface of placing the case is connected with the second rubber pad, the upper surface of second rubber pad and the bottom surface fixed connection of culture dish, place the inside wall fixedly connected with cardboard of case, the spout has been seted up to a side that the cardboard is close to each other, the inside joint of spout has the slide, the top fixedly connected with apron of slide, a side fixedly connected with connecting plate of apron. The utility model relates to a rational in infrastructure, it can pull out the apron through the handle, carries out cell culture to the inside of putting into the culture dish fat stem cell culture solution after that, by the meticulous cultivation to fat stem cell of a plurality of culture dishes, improves the efficiency of cultivateing the cell.

At present, the technology has certain defects: 1. the quality of the cultured adipose-derived stem cells is reduced due to the unsatisfactory temperature control of the culture medium during the culture of the adipose-derived stem cells; 2. no comparison of cultured adipose stem cells resulted in a decrease in the efficiency of the study.

SUMMERY OF THE UTILITY MODEL

The utility model provides an adipose stem cell exosome culture device mutually supports the temperature in can effectual control incubator through temperature controller, thermocouple and heater strip to can improve adipose stem cell's quality, the problem in the background can effectively be solved in rethread CCD camera and the image processor mutually supporting.

The utility model provides a specific technical scheme as follows:

the utility model provides an adipose-derived stem cell exosome culture device, which comprises an incubator, wherein a thermocouple is arranged on the left side of the inner side wall of the incubator, a heating wire is arranged on the right side of the inner side wall of the incubator, the input end of the heating wire is electrically connected with the output end of the thermocouple, the outer side wall of the incubator is provided with a temperature controller, the top of the inner side wall of the incubator is provided with a fixed block, the outer side wall of the fixed block is provided with two groups of springs which are symmetrically arranged on the outer side wall of the fixed block, the other end of the spring is provided with a U-shaped block, the outer side wall of the U-shaped block is connected with a CCD camera in a clearance way, image processor is installed to the lateral wall of incubator, hinged joint is passed through with the lateral wall of baffle to the lateral wall of incubator, the couple is installed to the baffle lateral wall, the buckle is installed to the incubator lateral wall.

Optionally, the output end of the temperature controller is electrically connected with the input end of the heating wire.

Optionally, the fixed block is concave and the spring is located in the concave position of the outer side wall of the fixed block.

Optionally, the input end of the image processor is electrically connected to the CCD camera.

Optionally, the input end of the image processor and the input end of the temperature controller are both electrically connected to the output end of the external power supply.

The utility model has the advantages as follows:

1. the utility model is practical, the operation is convenient and the using effect is good, the outer side wall of the baffle is connected with the outer side wall of the incubator through a hinge, the outer side wall of the baffle is provided with a hanging buckle, the outer side wall of the incubator is provided with a buckle, the hanging buckle is arranged in the buckle, the incubator is closed through the baffle, the outer side wall of the incubator is provided with a temperature controller, the output end of the temperature controller is electrically connected with the input end of the heating wire on the right side of the inner side wall of the incubator, so that the heating wire starts to work after the temperature controller is adjusted, the heating wire is heated in the incubator, and the output end of the heating wire is electrically connected with the thermocouple input end on the left side of the inner side wall of the incubator, so that the thermocouple detects the temperature and feeds back to the temperature controller, the temperature in the incubator is displayed by, thereby ensuring the quality of the cultured adipose-derived stem cells.

2. In the utility model, a fixed block is arranged on the top of the inner side wall of the incubator, the fixed block is in a concave shape, springs are welded on the outer side wall of the concave part, two groups of springs are symmetrically arranged on the outer side wall of the concave part, U-shaped blocks are arranged on the other ends of the two groups of springs, so that a CCD camera is arranged at the gap of the two groups of U-shaped blocks, the two groups of U-shaped blocks are close to each other when the CCD camera is not arranged, the CCD camera is fixed at the gap of the two groups of U-shaped blocks due to the extrusion of the springs after the CCD camera is arranged, an image processor is arranged on the outer side wall of the incubator, the input end of the image processor is connected with the CCD camera, the output end of the image processor is connected with the input end of an external computer, so that the image taken by the CCD camera is untimel, increasing the efficiency of the study.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

Fig. 1 is a schematic overall structure diagram of an adipose-derived stem cell exosome culture device according to an embodiment of the present invention;

fig. 2 is a schematic structural diagram of a fixing block of an adipose-derived stem cell exosome culture device according to an embodiment of the present invention;

in the figure: 1. an incubator; 2. a hinge; 3. a baffle plate; 4. a thermocouple; 5. hanging and buckling; 6. an image processor; 7. buckling; 8. a temperature controller; 9. heating wires; 10. a U-shaped block; 11. a fixed block; 12. a spring; 13. a CCD camera.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the present invention will be described in further detail with reference to the accompanying drawings, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

An adipose-derived stem cell exosome culture device according to an embodiment of the present invention will be described in detail with reference to fig. 1 to 2.

As shown in fig. 1-2, an adipose-derived stem cell exosome culture device comprises an incubator 1, wherein a thermocouple 4 is installed on the left side of the inner side wall of the incubator 1, a heating wire 9 is installed on the right side of the inner side wall of the incubator 1, the input end of the heating wire 9 is electrically connected with the output end of the thermocouple 4, a temperature controller 8 is installed on the outer side wall of the incubator 1, a fixed block 11 is installed at the top of the inner side wall of the incubator 1, a spring 12 is installed on the outer side wall of the fixed block 11, two groups of the spring 12 are symmetrically installed on the outer side wall of the fixed block 11, a U-shaped block 10 is installed at the other end of the spring 12, a CCD camera 13 is connected to the gap of the outer side wall of the incubator 1, an image processor 6 is installed on the outer side wall of the, couple 5 is installed to 3 lateral walls of baffle, buckle 7 is installed to 1 lateral wall of incubator. Wherein, thereby mutually support through temperature controller, thermocouple and heater strip and can effectual control incubator in the temperature to can improve the quality of adipose stem cell, rethread CCD camera and image processor mutually support.

In this embodiment, as shown in fig. 1, the output end of the temperature controller 8 is electrically connected to the input end of the heating wire 9. Wherein, the temperature of heater strip heating is conveniently controlled.

In this embodiment, as shown in fig. 2, the fixing block 11 is concave and the spring 12 is located at the concave position of the outer sidewall of the fixing block 11. Wherein, can be effectual fix the CCD camera.

In this embodiment, as shown in fig. 1, the input terminal of the image processor 6 is electrically connected to the CCD camera 13. Wherein, the culture degree of different time ends of the adipose-derived stem cells is convenient to observe.

In this embodiment, as shown in fig. 1-2, the input terminal of the image processor 6 and the input terminal of the temperature controller 8 are electrically connected to the output terminal of the external power source. Wherein, can effectively avoid the staff to electrocute.

It should be noted that, the utility model relates to an adipose-derived stem cell exosome culture device, in operation, switch on the external power supply, the lateral wall of baffle 3 is connected with the lateral wall of incubator 1 through hinge 2, the lateral wall of baffle 3 is installed with buckle 5, the lateral wall of incubator 1 is installed with buckle 7, install buckle 5 in buckle 7, thus seal incubator 1 through baffle 3, the lateral wall of incubator 1 is installed with temperature controller 8, the output of temperature controller 8 is electrically connected with the heater strip 9 input on the right side of the inner wall of incubator, thus adjust heater strip 9 behind temperature controller 8 and begin working, thus heater strip 9 heats in incubator 1, because the output of heater strip 9 is electrically connected with the left thermocouple 4 input of the inner wall of incubator 1, thus the thermocouple 4 feeds back to temperature controller 8 after detecting the temperature, the temperature in the incubator is displayed by the temperature controller 8, so that the temperature in the incubator 1 can be ensured to be constant, and the temperature of a culture medium can be ensured, so that the quality of cultured adipose-derived stem cells can be ensured, the fixed block 11 is installed at the top of the inner side wall of the incubator 1, the fixed block 11 is in a concave shape, the outer side wall of the concave part is welded with the springs 12, two groups of the springs 12 are symmetrically installed on the outer side wall of the concave part, the U-shaped block 10 is installed at the other end of each group of the springs 12, so that the CCD camera 13 is installed at the gap of the two groups of the U-shaped blocks 10, because the two groups of the U-shaped blocks 10 are close to each other when the CCD camera 13 is not installed, the CCD camera 13 is fixed at the gap of the two groups of the U-shaped blocks 10 due to the extrusion of the springs 12 after the CCD camera 13 is installed, the image processor 6 is installed on the outer side wall, therefore, the CCD camera 13 is used for shooting at random, the image processor 6 is used for displaying the pictures shot by the CCD camera 13 through the computer, comparison in different time periods can be carried out, the research efficiency is improved, and the external power supply is turned off after the use is finished so as to be used next time. The specific type of the component is a Rui-Gao CR21AL4 heating wire; a temperature controller for the YT-26 in the middle, west and far areas; platinum rhodium WRP-130 thermocouple; a Lingyun JC image processor; the image processing method comprises the following steps of (1) forming a cloud BFLY-U3-20S4C-CSCCD camera (light reflected by an object to be detected is refracted by an industrial lens to be on a photosensitive sensor (CCD or CMOS) to generate an analog current signal, the signal is converted into a digital signal through an analog-to-digital converter and then is transmitted to an image processor to obtain an image, and the digital signal is transmitted into a computer through an industrial camera communication interface to facilitate subsequent image processing and analysis).

The utility model relates to an adipose-derived stem cell exosome culture device 1 and a culture box; 2. a hinge; 3. a baffle plate; 4. a thermocouple; 5. hanging and buckling; 6. an image processor; 7. buckling; 8. a temperature controller; 9. heating wires; 10. a U-shaped block; 11. a fixed block; 12. a spring; 13. the CCD camera components are all standard or known to those skilled in the art, and their structure and principle are known to those skilled in the art through technical manuals or through routine experimentation.

It is apparent that those skilled in the art can make various changes and modifications to the embodiments of the present invention without departing from the spirit and scope of the embodiments of the present invention. Thus, if such modifications and variations of the embodiments of the present invention fall within the scope of the claims and their equivalents, the present invention is also intended to include such modifications and variations.

Claims

1. The utility model provides an adipose-derived stem cell exosome culture device, includes incubator (1), its characterized in that, thermocouple (4) are installed in incubator (1) inside wall left side, heater strip (9) are installed on the inside wall right side of incubator (1), the input of heater strip (9) and the output electric connection of thermocouple (4), temperature controller (8) are installed to the lateral wall of incubator (1), fixed block (11) are installed at the inside wall top of incubator (1), spring (12) are installed to the lateral wall of fixed block (11), spring (12) have two sets of be the lateral wall of symmetry installation at fixed block (11), U type piece (10) are installed to the other end of spring (12), the lateral wall gap connection of U type piece (10) has CCD camera (13), image processor (6) are installed to the lateral wall of incubator (1), the lateral wall of incubator (1) passes through hinge (2) with the lateral wall of baffle (3) and is connected, couple (5) are installed to baffle (3) lateral wall, buckle (7) are installed to incubator (1) lateral wall.

2. An adipose stem cell exosome culturing device according to claim 1, characterized in that the output end of the temperature controller (8) is electrically connected with the input end of the heating wire (9).

3. An adipose-derived stem cell exosome culturing device according to claim 1, characterized in that the fixed block (11) is concave in shape and the spring (12) is located at the concave position of the outer side wall of the fixed block (11).

4. An adipose-derived stem cell exosome culture device according to claim 1, wherein the input end of the image processor (6) is electrically connected with the CCD camera (13).

5. The adipose-derived stem cell exosome culture device according to claim 4, wherein the input end of the image processor (6) and the input end of the temperature controller (8) are both electrically connected with the output end of an external power supply.