CN212349092U

CN212349092U - Belt cleaning device of immobilized enzyme

Info

Publication number: CN212349092U
Application number: CN202020601642.8U
Authority: CN
Inventors: 陶荣盛; 原犇犇; 沈正权; 孙梁栋; 潘震华; 郑云; 胡海亮
Original assignee: Huzhou Yisheng Biotechnology Co Ltd
Current assignee: Huzhou Yisheng Biotechnology Co Ltd
Priority date: 2020-04-21
Filing date: 2020-04-21
Publication date: 2021-01-15
Anticipated expiration: 2030-04-21

Abstract

The utility model relates to an immobilized enzyme application, concretely relates to belt cleaning device of immobilized enzyme, include: the device comprises a cleaning tank body, a double-layer filter plate, an adjustable impurity separation port, an immobilized enzyme inverted port and a tank body rotating support, wherein the cleaning tank body is hinged with the tank body rotating support; the cleaning tank body is a tank body with an open top, the upper part of the cleaning tank body is a cylindrical sedimentation separation zone, the lower part of the cleaning tank body is a conical immobilized enzyme recovery zone, and the bottom of the cleaning tank body is provided with a liquid pumping pipe and a flushing pipe; the adjustable impurity separation port is arranged on the side wall of the sedimentation separation zone, and the immobilized enzyme inverted port is arranged on the side wall of the immobilized enzyme recovery zone. The utility model discloses a set up the impurity separation mouth that can slide adjusting from top to bottom, the level of layering face of finding out impurity and immobilized enzyme that can be more accurate, when accurately with impurity discharge, avoid the loss of enzyme.

Description

Belt cleaning device of immobilized enzyme

Technical Field

The utility model relates to an immobilized enzyme preparation and application, concretely relates to belt cleaning device of immobilized enzyme.

Background

An immobilized enzyme is an enzyme which has a catalytic action in a certain spatial range and can be repeatedly and continuously used. Generally, enzymatic reactions are carried out in an aqueous solution, and an immobilized enzyme is obtained by physically or chemically treating a water-soluble enzyme to render it insoluble in water but still enzymatically active. When the immobilized enzyme is prepared, crude enzyme liquid is often adopted for preparing the immobilized enzyme, and the crude enzyme liquid contains thallus fragments, foreign proteins and denatured enzymes which are not completely removed, and the thallus fragments, the foreign proteins and the denatured enzymes are generally called as solid impurities and need to be removed during cleaning; after the immobilized enzyme is used for a long time, the feed liquid contains denatured enzyme, fragments of the immobilized enzyme and carriers thereof, and some reactions are easy to breed sundry bacteria to form solid impurities, and the solid impurities also need to be removed by means of cleaning and the like.

In the prior art, manual cleaning is usually adopted, feed liquid containing immobilized enzyme is placed into a filter bag, the filter bag is shaken by a plurality of people, so that solid impurities float out from the bottom of the filter bag and the middle of the enzyme, then the shaking is stopped, and the solid impurities are quickly precipitated and slowly precipitated by the immobilized enzyme and are discharged along with water after being precipitated for a short time. The whole cleaning process needs manpower to be repeated for three or four times, and the cleaning is not thorough and needs to be frequently cleaned.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the problem in the background art and providing a cleaning device for immobilized enzyme.

The above technical purpose of the present invention can be achieved by the following technical solutions:

a washing apparatus for an immobilized enzyme, comprising: the device comprises a cleaning tank body, a double-layer filter plate, an adjustable impurity separation port, an immobilized enzyme inverted port and a tank body rotating support, wherein the cleaning tank body is hinged with the tank body rotating support; the cleaning tank body is a tank body with an open top, the upper part of the cleaning tank body is a cylindrical sedimentation separation zone, the lower part of the cleaning tank body is a conical immobilized enzyme recovery zone, and the bottom of the cleaning tank body is provided with a liquid pumping pipe and a flushing pipe; the adjustable impurity separation port is arranged on the side wall of the sedimentation separation zone, and the immobilized enzyme inverted port is arranged on the side wall of the immobilized enzyme recovery zone.

This application scheme, after pouring the feed liquid that contains the immobilized enzyme into the washing tank body, through the liquid suction pipe with the extraction of unnecessary liquid to standing after pouring water into the sedimentation separation district through the flushing pipe, under the effect of subsiding, impurity and immobilized enzyme can stratify, then take out upper impurity through adjustable impurity separation mouth.

Preferably, the adjustable impurity separation port comprises an impurity discharge pipe, an impurity discharge valve, a floating hose and a lifting positioning mechanism; the impurity discharge pipe is arranged on the outer side of the bottom of the side wall of the settling separation area, and the floating hose is arranged on the inner side of the settling separation area and communicated with the impurity discharge pipe.

Preferably, the lifting positioning mechanism comprises a slide rail, a slide hose pull rod and an observation window; the slide rail is arranged on the outer side of the side wall of the sedimentation separation area, the slide hose pull rod comprises a bar-shaped sliding block and a pull rod, the bar-shaped sliding block is connected with the top of the pull rod, the bar-shaped sliding block and the pull rod are respectively arranged on two sides of the side wall of the sedimentation separation area, and the bottom of the pull rod is fixed with an opening at the top end of the floating hose.

Preferably, the double-layer filter plate comprises an upper filter plate, a lower filter plate and a connecting column which are detachably connected, the upper filter plate is arranged in the sedimentation separation zone, the lower filter plate is arranged in the immobilized enzyme recovery zone, and the immobilized enzyme inverted port is arranged between the upper filter plate and the lower filter plate; the upper filter plate and the lower filter plate are both provided with a plurality of filter holes, the diameter of each filter hole of the upper filter plate is larger than that of the immobilized enzyme to be cleaned, and the diameter of each filter hole of the lower filter plate is smaller than that of the immobilized enzyme to be cleaned.

The double-layer filter plate structure ensures that immobilized enzyme cannot run off into a pipeline in the liquid extraction process by the bottom filter plate in the liquid extraction process, and has a certain filtering effect, so that the impurity separation effect is improved.

Preferably, both ends of the connecting column are provided with threads and connecting nuts, the connecting column is connected with the upper filter plate and the lower filter plate through the threads and the connecting nuts, and the distance between the upper filter plate and the lower filter plate is adjusted through the adjustment of the nuts.

Through above-mentioned structure, the upper and lower filter plate of different apertures can be replaced to the immobilized enzyme of different apertures washs.

Preferably, the outer ring of the upper filter plate is provided with threads, the inner side of the side wall of the sedimentation separation region is provided with threads matched with the threads, and the upper filter plate is fixed in the sedimentation separation region through the threads.

Preferably, sealing rings are arranged between the upper filter plate and the cleaning tank body, and between the lower filter plate and the cleaning tank body. The sealing ring can be compacted when the upper filter plate is screwed up by screw threads, so that the sealing property of the edge of the filter plate is ensured, and meanwhile, the connecting part of the connecting column and the upper and lower filter plates can be adjusted by screw threads, so that the upper and lower filter plates can be compacted tightly.

The thread sets up the outer lane at last filter plate, the sealing washer setting of upper and lower filter plate is in filter plate below edge, thereby the compaction sealing washer through the fastening of screw thread plays sealed effect, owing to be double-deck filter plate structure, and the mounted position of two internal filter plates of wash tank is fixed, so light leans on the upper filter plate thread to screw and probably appear down the filter plate not have the compaction and lead to the sealed not enough condition of effect of filter plate down, through the adjustable screw nut structure in spliced pole two, the interval of filter plate about can finely tuning, thereby guarantee when screwing up the upper filter plate, down the filter plate also compressed tightly, obtain good sealed effect.

Preferably, a locking screw and a position pointer are arranged on the strip-shaped sliding block, and the top of the position pointer is arranged at the observation window and is aligned with the top end of the floating hose.

Preferably, a liquid distributor is further arranged at the joint of the flushing pipe and the cleaning tank body, and the liquid distributor comprises a plurality of liquid spraying pipes with upward openings and communicated with the flushing pipe.

Preferably, the bottom of the cleaning tank body is also provided with an aeration pipe, and an outlet at the top of the aeration pipe is communicated with the liquid distributor, so that the flushing separation effect is enhanced.

Preferably, a rotating motor is arranged between the cleaning tank body and the tank body rotating support, so that the cleaning tank body can automatically rotate to discharge materials through an electric control system when the cleaning tank body discharges materials.

To sum up, the utility model has the advantages that:

1. a belt cleaning device of immobilized enzyme, through setting up the impurity separation mouth that can slide from top to bottom and adjust, can be more accurate find out the level-stepping face of impurity and immobilized enzyme, when accurately with impurity discharge, avoid the loss of enzyme.

2. A belt cleaning device of immobilized enzyme, further strengthen the separation effect through double-deck filter screen structure, have and go up to arrange miscellaneous, the function of filterable dual separation down.

Drawings

Fig. 1 is a schematic structural diagram of the present invention;

FIG. 2 is a schematic perspective exploded view of the present invention;

fig. 3 is a schematic cross-sectional view of the present invention.

Detailed Description

The following specific embodiments are merely illustrative of the present invention, and are not intended to limit the present invention, and those skilled in the art can make modifications of the present embodiments without inventive contribution as required after reading the present specification, but all the embodiments are protected by patent laws and protection within the scope of the present invention.

The present invention will be described in detail by way of examples with reference to the accompanying drawings.

Example 1:

referring to FIGS. 1 to 3, an apparatus for washing an immobilized enzyme comprises: the device comprises a cleaning tank body 1, a double-layer filter plate 2, an adjustable impurity separation port 3, an immobilized enzyme inverted port 4 and a tank body rotating support 6, wherein the cleaning tank body 1 is hinged with the tank body rotating support 6, and a rotating motor is arranged between the cleaning tank body 1 and the tank body rotating support 6; the cleaning tank body 1 is a tank body with an open top, the upper part of the cleaning tank body is a cylindrical sedimentation separation zone 11, the lower part of the cleaning tank body is a conical immobilized enzyme recovery zone 12, and the bottom of the cleaning tank body 1 is provided with a liquid pumping pipe 13 and a flushing pipe 14; the adjustable impurity separation port 3 is arranged on the side wall of the sedimentation separation zone 11, and the immobilized enzyme dumping port 4 is arranged on the side wall of the immobilized enzyme recovery zone 12.

The adjustable impurity separation port 3 comprises an impurity discharge pipe 31, an impurity discharge valve 32, a floating hose 33 and a lifting positioning mechanism 5; the impurity discharge pipe 31 is arranged outside the bottom of the side wall of the sedimentation separation zone 11, and the floating hose 33 is arranged inside the sedimentation separation zone 11 and communicated with the impurity discharge pipe 31.

The lifting positioning mechanism 5 comprises a slide rail 51, a slide hose pull rod 52 and an observation window 53; the slide rail 51 is arranged on the outer side of the side wall of the sedimentation separation area 11, the slide hose pull rod 52 comprises a strip-shaped slide block 521 and a pull rod 522, the strip-shaped slide block 521 is connected with the top of the pull rod 522 and is respectively arranged on two sides of the side wall of the sedimentation separation area 11, and the bottom of the pull rod 522 is fixed with an opening at the top end of the floating hose 33.

The double-layer filter plate 2 comprises an upper filter plate 21, a lower filter plate 22 and a connecting column 23 which are detachably connected, wherein the upper filter plate 21 is arranged in the sedimentation separation zone 11, the lower filter plate 22 is arranged in the immobilized enzyme recovery zone 12, and the immobilized enzyme inverted port 4 is arranged between the upper filter plate 21 and the lower filter plate 22; the upper filter plate 21 and the lower filter plate 22 are both provided with a plurality of filter holes, the diameter of the filter holes of the upper filter plate 21 is larger than that of the immobilized enzyme to be cleaned, and the diameter of the filter holes of the lower filter plate 22 is smaller than that of the immobilized enzyme to be cleaned.

The connecting column 23 is provided with threads and connecting nuts at both ends, the connecting column 23 is connected with the upper filter plate 21 and the lower filter plate 22 through the threads and the connecting nuts, and the distance between the upper filter plate 21 and the lower filter plate 22 is adjusted through the adjustment of the nuts.

The outer ring of the upper filter plate 21 is provided with screw threads, the inner side of the side wall of the sedimentation separation zone 11 is provided with screw threads matched with the screw threads, the upper filter plate 21 is fixed in the sedimentation separation zone 11 through the screw threads, and sealing rings are arranged between the upper filter plate 21 and the lower filter plate 22 and the cleaning tank body 1.

The bar-shaped sliding block 521 is provided with a locking screw 523 and a position pointer 524, and the top of the position pointer 524 is arranged at the observation window 53 and is aligned with the top end of the floating hose 33.

The specific process of cleaning the immobilized enzyme by adopting the device is that the immobilized enzyme feed liquid to be cleaned is poured into the cleaning tank body 1. Then the liquid extracting pipe 13 is opened to extract the redundant liquid except the immobilized enzyme, and the immobilized enzyme cannot be extracted by the pipeline because the pore diameter of the lower filter plate 22 is small. Then, the flushing pipe 14 is opened to inject water into the cleaning tank body 1 and then the cleaning tank body is kept still, because the volume of impurities is relatively small, under the action of sedimentation, the impurities and immobilized enzyme can be layered, at the moment, a layered surface is observed through an observation window 53 made of glass (an illuminating lamp can be arranged in the observation window to assist in observation), the strip-shaped sliding block 521 is slid, so that the top of the floating hose 33 is positioned at an interface, then the impurity discharge valve 32 is opened to discharge upper impurity liquid from the sedimentation separation area 11, and after the impurity liquid at the inlet at the top of the floating hose 33 is completely discharged, the liquid suction pipe 13 is opened again to suck out redundant liquid. At this time, the immobilized enzyme falls into the immobilized enzyme recovery area 12 from the filtration pores of the upper filter plate 21, and finally the cleaning tank 1 is rotated and the sealing door plate at the immobilized enzyme pouring port 4 is opened, so that the solid immobilized enzyme cleaned in the immobilized enzyme recovery area 12 is poured out from the immobilized enzyme pouring port 4 and collected.

Example 2:

the difference from the embodiment 1 is that, according to fig. 3, a liquid distributor 141 is further provided at the connection between the flushing pipe 14 and the cleaning tank 1, and the liquid distributor includes a plurality of liquid spraying pipes with upward openings and communicated with the flushing pipe 14 to improve the flushing effect.

Example 3:

the difference from the embodiment 2 is that the bottom of the cleaning tank body 1 is also provided with an aeration pipe 15, the outlet at the top of the aeration pipe 15 is communicated with the liquid distributor 141, and when the liquid is used for washing, aeration is added simultaneously, so that the washing effect can be improved, and the separation capacity can be enhanced.

Claims

1. A washing apparatus for an immobilized enzyme, comprising: the device comprises a cleaning tank body (1), a double-layer filter plate (2), an adjustable impurity separation port (3), an immobilized enzyme inverted port (4) and a tank body rotating support (6), wherein the cleaning tank body (1) is hinged with the tank body rotating support (6);

the cleaning tank body (1) is a tank body with an open top, the upper part of the cleaning tank body is a cylindrical settling separation zone (11), the lower part of the cleaning tank body is a conical immobilized enzyme recovery zone (12), and the bottom of the cleaning tank body (1) is provided with a liquid pumping pipe (13) and a flushing pipe (14);

the adjustable impurity separation port (3) is arranged on the side wall of the sedimentation separation zone (11), and the immobilized enzyme inverted port (4) is arranged on the side wall of the immobilized enzyme recovery zone (12).

2. The washing device of immobilized enzyme according to claim 1, wherein the adjustable impurity separation port (3) comprises an impurity discharge pipe (31), an impurity discharge valve (32), a floating hose (33) and a lifting positioning mechanism (5); the impurity discharge pipe (31) is arranged on the outer side of the bottom of the side wall of the settling separation area (11), and the floating hose (33) is arranged on the inner side of the settling separation area (11) and communicated with the impurity discharge pipe (31).

3. The washing device of immobilized enzyme according to claim 2, wherein the lifting and positioning mechanism (5) comprises a slide rail (51), a slide hose pull rod (52) and an observation window (53); slide rail (51) are established the subside separation region (11) lateral wall outside, slip hose pull rod (52) include, bar slider (521) and pull rod (522), bar slider (521) with both tops of pull rod (522) link to each other, and set up respectively the both sides of subside separation region (11) lateral wall, pull rod (522) bottom with the flexible pipe (33) top opening part of floating is fixed mutually.

4. The washing device of immobilized enzyme according to claim 1, wherein the double-layer filter plate (2) comprises an upper filter plate (21), a lower filter plate (22) and a connecting column (23) which are detachably connected, the upper filter plate (21) is arranged in the sedimentation separation zone (11), the lower filter plate (22) is arranged in the immobilized enzyme recovery zone (12), and the immobilized enzyme pouring port (4) is arranged between the upper filter plate (21) and the lower filter plate (22); the upper filter plate (21) and the lower filter plate (22) are both provided with a plurality of filter holes, the diameter of the filter hole of the upper filter plate (21) is larger than that of the immobilized enzyme required to be cleaned, and the diameter of the filter hole of the lower filter plate (22) is smaller than that of the immobilized enzyme required to be cleaned.

5. The washing device of immobilized enzyme according to claim 4, wherein the connecting column (23) is provided with screw threads and connecting nuts at two ends, the connecting column (23) is connected with the upper filter plate (21) and the lower filter plate (22) through the screw threads and the connecting nuts, and the distance between the upper filter plate (21) and the lower filter plate (22) is adjusted through the adjustment of the nuts.

6. The device for cleaning the immobilized enzyme according to claim 5, wherein the outer ring of the upper filter plate (21) is provided with screw threads, the inner side of the side wall of the sedimentation separation zone (11) is provided with screw threads matched with the inner side, and the upper filter plate (21) is fixed in the sedimentation separation zone (11) through the screw threads.

7. The washing device for immobilized enzyme according to claim 6, wherein sealing rings are arranged between the upper filter plate (21) and the lower filter plate (22) and the washing tank (1).

8. The apparatus for washing an immobilized enzyme according to claim 3, wherein the bar-shaped slider (521) is provided with a locking screw (523) and a position pointer (524), and the top of the position pointer (524) is arranged at the observation window (53) and is aligned with the top end of the floating hose (33).

9. The washing device of immobilized enzyme according to claim 1, wherein a liquid distributor (141) is further provided at the junction of the washing tube (14) and the washing tank (1), and the liquid distributor comprises a plurality of liquid spraying tubes with upward openings and communicated with the washing tube (14).

10. The washing device for immobilized enzyme according to claim 9, wherein the bottom of the washing tank (1) is further provided with an aeration pipe (15), and the top outlet of the aeration pipe (15) is communicated with the liquid distributor (141).