CN212341174U - Large-traffic tail gas analysis device of binary channels for microorganism high density culture - Google Patents
Large-traffic tail gas analysis device of binary channels for microorganism high density culture Download PDFInfo
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- CN212341174U CN212341174U CN202021437501.3U CN202021437501U CN212341174U CN 212341174 U CN212341174 U CN 212341174U CN 202021437501 U CN202021437501 U CN 202021437501U CN 212341174 U CN212341174 U CN 212341174U
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Abstract
The utility model relates to a two-channel large-flow tail gas analysis device for high-density culture of microorganisms, a tail gas purification sampling module comprises a tail gas inlet pipe, an inlet valve, a filter inlet valve, a dewatering filter, a filter outlet valve, a sampling gas switching valve, a large-flow air pump and a gas mass flowmeter which are sequentially connected in series; the gas mass flow meters of the tail gas purification sampling modules are respectively connected with a tail gas parameter detection module; a channel switching valve is connected between the outlet ends of the air inlet valves of the two tail gas purification sampling modules; the fan is connected with the heating bag, and the heating bag is respectively connected with a water removal filter of a tail gas purification sampling module; the drainage pipeline interface of the water removal filter is connected with the filter exhaust valve and the filter drain valve through the drainage pipeline interface. The utility model discloses a microorganism high density is cultivateed with large-traffic tail gas analysis device response speed of binary channels is fast, long service life, can realize that single channel or binary channels detect and carry out fermentation metabolism regulation and control for a long time, complicated composition on line.
Description
Technical Field
The utility model relates to a microorganism high density is cultivateed with large-traffic tail gas analysis device of binary channels belongs to fermentation tail gas detection and analysis field.
Background
In the process of high-density aerobic biological fermentation production, when bioengineering bacteria carry out production metabolism in a fermentation tank, a large amount of tail gas is generated, and partial fermentation metabolites are discharged along with the tail gas and accompanied with special unpleasant gas. CO in microbial exhaled breath2、O2The change of the odorous gas content of heterocyclic compounds such as volatile aldehydes, amines, alcohols, indole and furans can reflect the change of thallus metabolism, particularly in the high-density culture process, the real-time display of tail gas analysis is particularly important, and the method has very important significance in fermentation metabolism regulation.
At present, fermentation tank tail gas analyzers circulating in the market have the following problems for the conditions of complex fermentation tail gas components and high humidity: 1. the parameter detection accuracy is not sufficient due to the fact that a tail gas condensation method is commonly adopted for water removal. Because fermentation tail gas humidity is great, if direct detection, can form the condensation on the probe guard shield of sensor, influence detection effect. The condensing method is adopted to remove the moisture in the fermentation tail gas, and the defect is that CO2, O2 and various secondary odor compounds in the tail gas are dissolved in condensed water in the low-temperature condensing process, so that the detected parameters are low, and the fermentation regulation and control judgment is influenced. 2. The tail gas drying method removes water, and the gas flow is insufficient. The silica gel drying agent method for removing tail gas moisture is commonly used in gas pretreatment of a gas phase mass spectrometer, but the drying agent method is not suitable for removing water from high-flow tail gas, the maximum treatment flow of a conventional drying tube with the diameter of 38mm and the length of 240mm is only 5L/Min, and the industrial requirement is far not met. 3. The real-time performance is insufficient. A traditional fermentation tail gas analyzer adopts a multi-channel serial detection method, and after the detection of a channel 1 is finished, the detection is switched to a channel 2 and a channel 3 for detection. The detection can be started only when the tail gas of other channels is completely replaced and the flow is stable, so that the detection time is long, the channel response is slow, the detection parameters are delayed, and the method is not suitable for large-scale culture of high-density microorganisms.
Disclosure of Invention
The utility model aims to solve the technical problem that a simple structure, convenient operation, large-traffic tail gas dewatering are effectual, response speed is fast, long service life, can realize that single channel or binary channels detect and carry out fermentation metabolism regulation and control's tail gas analysis and control system for a long time, complicated composition on line.
A double-channel large-flow tail gas analysis device for high-density culture of microorganisms comprises two tail gas purification sampling modules, two tail gas parameter detection modules, a fan and a heating bag, wherein each tail gas purification sampling module comprises a tail gas inlet pipe, an inlet valve, a filter inlet valve, a dewatering filter, a filter outlet valve, a sampling gas switching valve, a large-flow gas pump and a gas mass flowmeter which are sequentially connected in series; the gas mass flowmeters of the two tail gas purification sampling modules are respectively connected with a tail gas parameter detection module; a channel switching valve is connected between the outlet ends of the air inlet valves of the two tail gas purification sampling modules; the air outlet port of the fan is connected with the heating bag through a pipeline, and the heating bag is respectively connected with a water removal filter of a tail gas purification sampling module through two reverse drying air inlet valves; the drainage pipeline interface of the water removal filter is connected with the filter exhaust valve and the filter drain valve through the drainage pipeline interface.
The double-channel large-flow tail gas analysis device for the high-density culture of the microorganisms is further designed in that the water removal filter is a membrane filter.
A microorganism high density is cultivateed with large-traffic tail gas analysis device of binary channels, its further design lies in, membrane filter includes filter housing and the fixed filter core that sets up in filter housing inside, the casing is connected with the filter intake pipe, the filter core is connected with the filter outlet duct that extends to the casing outside.
The utility model provides a big flow-rate tail gas analytical equipment of binary channels for microorganism high density culture, its further design lie in, the outer wall of filter housing is provided with the soft cover of silica gel electrical heating.
The double-channel large-flow tail gas analysis device for the high-density culture of the microorganisms is further designed in that the filter element is a PTFE waterproof membrane, a plurality of small holes are uniformly and densely distributed in the membrane, and the pore diameter of each small hole is smaller than that of water molecules, so that the circulation of water and particles can be prevented.
The double-channel large-flow tail gas analysis device for the high-density culture of the microorganisms is further designed in that the filter shell is provided with a temperature interface, and the temperature interface is provided with a temperature sensing element.
A microorganism high density is cultivateed with large-traffic tail gas analytical equipment of binary channels, its further design lies in, tail gas parameter detection module is including detecting the box and setting up electrochemical oxygen sensor, infrared ray carbon dioxide sensor, smell sensor group and the detection chamber trap in detecting the box, it is provided with the tail gas vent to detect the box.
The two-channel large-flow tail gas analysis device for high-density culture of microorganisms is further designed in that the odor sensor group comprises an H2S hydrogen sulfide sensor, an H20 humidity sensor, an NH3 ammonia sensor and an SO2 sulfur dioxide sensor.
The double-channel large-flow tail gas analysis device for the high-density culture of the microorganisms is further designed in such a way that a plurality of partition plates are arranged in the detection box body in a staggered mode, and gas flow channels are formed among the partition plates; the distance between the clapboards positioned in the middle of the detection box body is larger than the distance between the clapboards positioned at other parts of the detection box body, so that an airflow deceleration area is formed inside the detection box body, and the electrochemical oxygen sensor, the infrared carbon dioxide sensor and the odor sensor group are arranged in the airflow deceleration area.
The utility model discloses a microorganism high density is cultivateed with large-traffic tail gas analysis device of binary channels simple structure, convenient operation, response speed are fast, long service life, can realize that single channel or binary channels detect and carry out fermentation metabolism regulation and control for a long time, complicated composition on line.
Drawings
Fig. 1 is a block diagram 1 of an application state structure according to an embodiment of the present invention.
FIG. 2 is a schematic view of the membrane filtration dehydrator
FIG. 3 is a schematic view of the structure of the filter element seat of the membrane filter of the present invention
FIG. 4 is a schematic view of a filter element structure of a membrane filter of the present invention
FIG. 5 is a schematic view showing the flow direction of the exhaust gas and the flow direction of the dry hot gas during the operation of the No. 1 membrane filter in mode 1
FIG. 6 is a schematic view showing the flow direction of the exhaust gas and the flow direction of the drying hot gas during the operation of the 2# membrane filter in mode 1
FIG. 7 is a schematic view of the exhaust gas flow during operation of the membrane filter in mode 2
FIG. 8 is a schematic view of a structure of a tail gas detection chamber
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
A double-channel large-flow tail gas analysis device for high-density culture of microorganisms comprises two tail gas purification and sampling modules, two tail gas parameter detection modules, a fan and a heating bag; the system comprises two tail gas purification sampling modules, namely a 1# tail gas purification sampling module and a 2# tail gas purification sampling module, wherein the 1# tail gas purification sampling module comprises a tail gas inlet pipe 1, a pressure-sensitive element 2, an inlet valve 3, a filter inlet valve 4, a dewatering filter 11, a filter outlet valve 16, a sampling gas switching valve 25, a large-flow gas pump 10 and a gas mass flowmeter 17 which are sequentially connected in series; the 2# tail gas purification sampling module comprises a tail gas inlet pipe 5, a pressure sensing element 6, an inlet valve 7, a filter inlet valve 8, a dewatering filter 11 ', a filter outlet valve 16', a sampling gas switching valve 25, a high-flow gas pump 10 'and a gas mass flowmeter 17' which are sequentially connected in series; specifically, the water removal filter is a membrane filter.
The gas mass flow meters of the two tail gas purification sampling modules are respectively connected with a tail gas parameter detection module, and the two tail gas layer number detection modules are respectively arranged in the detection box body 24 and the detection box body 24'; a channel switching valve 9 is connected between the outlet ends of the air inlet valves of the two tail gas purification sampling modules; the blower is a Roots blower 21, the air outlet port of the Roots blower is connected with the heating bag 18 through a pipeline, and the heating bag is respectively connected with a water removal filter of a tail gas purification sampling module through two reverse drying air inlet valves 15 (and 15'); the drain line of the water removal filter 11 is connected to a filter exhaust valve 13 and a filter drain valve 14. Accordingly, the drain line connection of the water removal filter 11 ' is connected via this drain line connection to the filter outlet valve 13 ', the filter drain valve 14 '.
The embodiment has 2 working modes: the mode 1 is that the single-channel air inlet and inlet channels 1 and 2 can be selected, and the membrane filter switches the filtration. Mode 2 is two-pass individual inlet air, with continuous filtration through individual membrane filters.
Specifically, mode 1: the single channel is admitted air, and the membrane filter switches the filtration, and reserve filter reverse hot-blast stoving has two states.
State 1: when starting, the default is to use 'fermentation tail gas inlet 1 #' as an air inlet, a 1# membrane filter and a detection cavity to work, and the flow direction of the fermentation tail gas and the flow direction of the drying tail gas are shown in figure 5. The large flow air pump 10 is started, the intake valve 3, the intake valve 4, and the 1# passage detection intake valve 16 are opened, and the passage switching valve 25 and the 1# filter exhaust valve 13 are closed. Fermentation tail gas enters the membrane filter 11 through the inlet 1# gas port 1 and the 1# pressure sensing element 2 along the valve opening channel, and the tail gas enters the 1# detection box body 24 through the gas mass flowmeter 17 after being purified and filtered by the membrane filter 11. The small water molecules intercepted by the No. 1 membrane filter are collected into water drops, and the water drops flow out through a filter drainage trap. Meanwhile, the heating bag 18 and the Roots blower 21 are started, the membrane filter 11 ' is opened, the air inlet valve 15 ' and the 2# membrane filter exhaust valve 13 ' are dried in the reverse direction, and hot air passes through the 2# membrane filter element and is exhausted from the filter exhaust valve.
When the gas mass flow meter detects that the current tail gas inlet flow is smaller than the flow set value, the gas inlet and the detection cavity are unchanged, the tail gas is switched to the mold filter 11' for filtering, the mold filter 11 is dried reversely, and the state 2 is entered.
The flow direction of the fermentation tail gas and the drying tail gas is shown in figure 6. The large flow air pump 10 is started, the 1# channel valve 3, the 1# channel air inlet switching valve 9 and the 2# channel air inlet control valve 8 are opened, the channel switching valve 25 and the 1# channel detection air inlet valve 16 are opened, and the 1# filter air inlet valve 14, the 2# membrane filter exhaust valve 13' and the 2# membrane filter reverse drying air inlet valve 16 are closed. The fermentation tail gas enters a No. 2 membrane filter 11' through a No. 1 gas inlet 1 and a No. 1 pressure sensing element 2 along a valve opening channel, and the fermentation tail gas enters a No. 1 detection box body 24 through a No. 1 mass gas mass flowmeter 17 after being purified and filtered by the No. 2 membrane filter. And small water molecules intercepted by the No. 2 membrane filter are collected into water drops, and flow out through a filter drainage trap. Meanwhile, the heating bag 18 and the Roots blower 21 are started, the 1# membrane filter reverse drying air inlet valve 15' and the 1# membrane filter exhaust valve 13 are opened, the 1# membrane filter air outlet control valve 12 is closed, and hot air passes through the 1# membrane filter element and is exhausted from the filter exhaust valve.
When the gas mass flow meter detects that the current tail gas inlet flow is smaller than a flow set value, the gas inlet and the detection cavity are unchanged, the tail gas is switched to the 1# mode filter for filtering, the 2# mode filter is dried reversely, and the state is entered into 1.
The mode 1 is suitable for industrial, large-flow, long-time and large-tail gas dewatering and filtering, and can fundamentally solve the problem of membrane filter blockage.
Mode 2 is two-pass individual inlet air, with continuous filtration through individual membrane filters. The flow direction of fermentation tail gas is shown in FIG. 7
"fermentation tail gas intake 1 #" as the air intake: the large flow air pump 10 is started, the 1# channel valve 3, the 1# channel intake valve 4, and the 1# channel detection intake valve 1216 are opened, and the channel switching valve 25, the 1# filter exhaust valve 13, and the 1# filter reverse drying intake valve 15 are closed. Fermentation tail gas enters a No. 1 membrane filter 11 through a No. 1 gas inlet 1 and a No. 1 pressure sensing element 2 along a valve opening channel, and the fermentation tail gas enters a No. 1 detection box body 24 through a No. 1 mass gas mass flowmeter 17 after being purified and filtered by the No. 1 membrane filter. The small water molecules intercepted by the No. 1 membrane filter are collected into water drops and discharged through the No. 1 membrane filter drainage trap 14.
"fermentation tail gas inlet 2 #" is used as an air inlet: and starting the large-flow air pump 10 ', opening the 2# channel valve 7, the 2# channel air inlet valve 8 and the 2# channel detection air inlet valve 16', and closing the channel switching valve 25, the 2# filter exhaust valve 13 'and the 1# filter reverse drying air inlet valve 15'. The fermentation tail gas enters a No. 2 membrane filter 11 ' through a No. 2 gas inlet 5 and a No. 2 pressure sensing element 6 along a valve opening channel, and the tail gas is purified and filtered by the No. 2 membrane filter and then enters a No. 2 detection box body 24 ' through a No. 2 mass gas mass flowmeter 17 '. The small water molecules intercepted by the No. 2 membrane filter are collected into water drops and discharged through the No. 2 membrane filter drainage trap 14'.
As shown in fig. 2-4, the filter casing is composed of a stainless steel cylindrical upper casing F and a lower casing a, a stainless steel large clamp groove is arranged at the joint of the upper casing and the lower casing, the stainless steel large clamp groove is clamped and locked by a heavy large clamp, and a silica gel sealing ring C is arranged in the filter casing. Taking the membrane filter 11 as an example, the bottom of the lower shell is provided with a drainage pipeline interface, and a filter exhaust valve 13 and a drain valve 14 are connected below the drainage pipeline interface. The left side of the lower shell is provided with a filter air inlet pipe B which is communicated with the inside of the filter. The filter outlet pipe G is arranged on the right side of the lower shell and communicated with the filter element base D inside the filter, the filter element base is provided with a groove-shaped filter element clamping groove, a waist-shaped notch is cut in the upper end face of the clamping groove, and a waist-shaped protruding fixing piece of the matched filter element is arranged. The top of the upper shell is provided with a temperature interface which is matched with a temperature sensing element 12.
The outer wall of the shell of the filter is provided with a silica gel electric heating soft sleeve. The filter core adopts PTFE material water proof membrane, and the balanced density of epimembranal several apertures, this aperture of aperture is less than the hydrone, can prevent the circulation of water and granule. The filter housing is provided with a temperature interface, which, as shown in connection with fig. 1, is provided with a temperature sensitive element 12.
A tail gas parameter detection module is including detecting box 24 and setting up electrochemistry oxygen sensor 19, infrared ray carbon dioxide sensor 20, smell sensor group 22 and detection chamber trap 23 in detecting the box, it is provided with the tail gas vent to detect the box. The other tail gas parameter detection module comprises a detection box body 24 ', an electrochemical oxygen sensor 19 ', an infrared carbon dioxide sensor 20 ', a smell sensor group 22 ' and a detection cavity drain valve 23 ' which are arranged in the detection box body, and the detection box body is provided with a tail gas exhaust port.
The odor sensor group comprises a H2S hydrogen sulfide sensor, an H20 humidity sensor, an NH3 ammonia sensor and an SO2 sulfur dioxide sensor.
As shown in fig. 8, a plurality of partition plates are arranged in the detection box body in a staggered manner, and a gas flow channel is formed between the partition plates; the distance between the clapboards in the middle of the detection box body is larger than that between the clapboards in other parts of the detection box body, so that an airflow deceleration area is formed in the detection box body, and the electrochemical oxygen sensor 19, the infrared carbon dioxide sensor 20 and the odor sensor group 22 are arranged in the airflow deceleration area.
In the application of the embodiment, the digital control system is connected in a matching way and comprises a fermentation tank detection/control unit, a fermentation tail gas detection control unit, a biomass operation unit, an alarm processing unit and a remote expert service system. The hardware system of the system consists of a signal preprocessing circuit, a human-computer interface, a CPU (central processing unit), a signal acquisition module, a valve group control module, a flow regulation module and a network communication module. The signal input end of the signal preprocessing circuit is connected with various sensors in the detection system, the signal acquisition module is in butt joint with the signal output end of the signal preprocessing circuit, the output end of the power supply circuit is connected with the power supply end of the system, and the CPU central processing unit is connected with the modules through buses. The CPU is provided with a fermentation tail gas detection control unit, a biomass operation unit and an alarm processing unit. The network communication module is connected with the fermentation tank detection/control unit and the remote expert service system.
The high-flow air pump is started, fermentation tail gas is filtered and purified through the air inlet pipe, the air inlet channel control valve and the membrane filter, then enters the detection cavity, electric signals of various gases are detected through the detection system, the electric signals are accessed into the digital control system, and are sent to the touch display screen and the remote server system after being subjected to signal standardization processing.
The digital control system is used for detecting electric signals of various sensors, filtering and amplifying the electric signals into standard signals, accessing the standard signals into a signal acquisition module, displaying tail gas parameters on a human interface after software engineering conversion and parameter compensation processing, and simultaneously reading parameter measurement values of the fermentation tank through a network interface and carrying out biomass parameter operation. Meanwhile, the operating parameters of the fermentation tank such as sugar supplement, alkali supplement and stirring speed are finely adjusted according to the biomass material parameters.
The biomass operation unit reads the physical parameters related to the biomass operation participated in the fermentation tank culture process from the fermentation tank detection/control unit through a system communication interface: ventilation, volume, dissolved oxygen value, temperature, pH, stirring speed, etc. And combining the contents of fermentation tail gas metabolism parameters O2 and CO2, and obtaining Oxygen Uptake Rate (OUR), CO2 release rate CER, specific growth rate u, respiratory quotient RQ, oxygen transfer coefficient Kla1 and respiratory intensity QO2 by soft computing. The device has the advantages of quick response to the parameter regulation and control of the fermentation tank high-density culture process, accurate and reliable data, good stability and realization of continuous online measurement of fermentation tail gas components during high-density culture of microorganisms.
The remote expert service system acquires the measured values of the fermentation tank parameters and the tail gas parameters through the network interface, performs multiple analysis on the data, judges the working condition interval where the fermentation is located through comparison of a large amount of data of the model database, and gives a diagnosis result and a metabolic regulation and control suggestion to the client, and an operator only needs to finely regulate and control the fermentation operation parameters through the fermentation control unit according to the suggestion.
Claims (9)
1. A dual-channel large-flow tail gas analysis device for high-density culture of microorganisms is characterized by comprising two tail gas purification sampling modules, two tail gas parameter detection modules, a fan and a heating bag, wherein each tail gas purification sampling module comprises a tail gas inlet pipe, an air inlet valve (3), a filter air inlet valve (4), a dewatering filter (11), a filter air outlet valve (16), a sampling gas switching valve (25), a large-flow air pump (1) and a gas mass flowmeter (17) which are sequentially connected in series; the gas mass flowmeters of the two tail gas purification sampling modules are respectively connected with a tail gas parameter detection module; a channel switching valve is connected between the outlet ends of the air inlet valves of the two tail gas purification sampling modules; the air outlet port of the fan is connected with the heating bag through a pipeline, and the heating bag is respectively connected with a water removal filter of a tail gas purification sampling module through two reverse drying air inlet valves; the drainage pipeline interface of the water removal filter is connected with a filter exhaust valve (13) and a filter drain valve (14) through the drainage pipeline interface.
2. The dual-channel high-flow exhaust gas analyzer for high-density cultivation of microorganisms according to claim 1, wherein the water removal filter is a membrane filter.
3. The dual-channel large-flow tail gas analysis device for the high-density culture of the microorganisms according to claim 2, wherein the membrane filter comprises a filter shell and a filter element fixedly arranged inside the filter shell, the shell is connected with a filter inlet pipe, and the filter element is connected with a filter outlet pipe extending to the outside of the shell.
4. The dual-channel high-flow tail gas analysis device for the high-density culture of the microorganisms according to claim 3, wherein a silica gel electric heating soft sleeve is arranged on the outer wall of the filter shell.
5. The dual-channel large-flow tail gas analysis device for high-density culture of microorganisms according to claim 3, wherein the filter element is a PTFE waterproof membrane, a plurality of small holes are uniformly and densely distributed on the membrane, and the pore diameter of the small holes is smaller than that of water molecules, so that the circulation of water and particles can be prevented.
6. The dual-channel high-flow exhaust gas analysis device for high-density culture of microorganisms according to claim 3, wherein the filter housing is provided with a temperature interface provided with a temperature sensing element.
7. The dual-channel large-flow tail gas analysis device for the high-density culture of the microorganisms according to claim 3, wherein the tail gas parameter detection module comprises a detection box body (24), and an electrochemical oxygen sensor (19), an infrared carbon dioxide sensor (20), an odor sensor group (22) and a detection cavity drain valve (23) which are arranged in the detection box body, and the detection box body is provided with a tail gas exhaust port.
8. The dual-channel large-flow tail gas analysis device for high-density culture of microorganisms according to claim 7, wherein the odor sensor group comprises a H2S hydrogen sulfide sensor, an H20 humidity sensor, an NH3 ammonia sensor and an SO2 sulfur dioxide sensor.
9. The dual-channel high-flow tail gas analysis device for high-density culture of microorganisms according to claim 7, wherein a plurality of partition plates are arranged in the detection box body in a staggered manner, and a gas flow channel is formed between the partition plates; the distance between the partition boards positioned in the middle of the detection box body is larger than that between the partition boards positioned in other parts of the detection box body, so that an airflow deceleration area is formed inside the detection box body, and the electrochemical oxygen sensor (19), the infrared carbon dioxide sensor (20) and the odor sensor group (22) are arranged in the airflow deceleration area.
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