CN212328341U - Pipette of supplementary bacterial colony mixture - Google Patents

Abstract

The utility model provides a pipette of supplementary bacterial colony mixture, aims at solving among the prior art and carries out the operation cost of bacterial colony mixing higher and consuming time longer in the pipette, is difficult to popularize to the actual technical problem of production. The utility model discloses a first body, the one end of first body is equipped with the first mouth of getting liquid, the cover is equipped with the second body in the first body, the second body is kept away from the first one end of getting liquid mouth and is used for connecting the aurilave, the other end of second body is equipped with the second and gets liquid mouth, the open end of the first liquid mouth of getting and the open end looks parallel and level of the second liquid mouth of getting, the outer wall of second body constitutes first cavity with the inner wall of first body, the inside of second body is the second cavity, be equipped with the overflow punch combination of intercommunication first cavity and second cavity on the second body.

Description

Pipette of supplementary bacterial colony mixture

Technical Field

The utility model relates to a pipette technical field especially relates to a pipette of supplementary bacterial colony mixture.

Background

Rapid and accurate processing of hospital patient samples is critical to the diagnosis of disease and the use of correct bactericidal drugs. Currently, microbiologists collect patient samples and place them in petri dishes filled with media, then incubate the dishes in a temperature controlled environment, remove some or all of the colonies from the petri dishes with a cotton swab, metal stick, or other instrument when they become visible in the petri dishes, and insert them into tubes filled with saline solution, then mix the colonies with the saline solution in the tubes, usually by hand, then place the mixture in the instrument, incubate and detect bacterial growth at various drug types and concentrations, and then find the most effective drug. Because bacterial colony inhomogeneous distribution in the culture dish, after using traditional pipette to carry out bacterial colony transfer, the bacterial colony in the test tube is inhomogeneous distribution equally, because many bacterial colonies have oily composition and only partially dissolve, so the bacterial colony after with the salt solution contact is difficult to carry out the mixing operation, and based on this, the mixing operation of bacterial colony needs to be carried out in culture dish or pipette, but because the environment of culture dish is the natural growth environment of simulation bacterial colony, it is difficult to realize to carry out the mixing operation in the culture dish, and based on this, it is very necessary to provide a pipette that can assist the bacterial colony to mix.

Chinese patent application No. CN102123786A discloses a mixing pipette, comprising: a generally cylindrical, elongated tubular body comprising: an internal passageway having a tube inner diameter, a first opening at a first end of the tube body and a second opening at a second end of the tube body; wherein the tubular body further comprises an inward deformation between the first end and the second end to form a narrow slit within the internal passage of the pipette, one side of the slit having an inclined inlet and the other side of the slit having an inclined outlet, the inclined inlet and the inclined outlet forming a transition between the tube inner diameter and the narrow slit.

Above-mentioned patent can assist the mixture of realizing the bacterial colony, and its problem lies in, above-mentioned pipette is when carrying out the mixture of bacterial colony, actually is through the bacterial colony in the plus air supply disturbance pipette, though the bacterial colony is mixable abundant, the operating cost of bacterial colony mixing is higher and consuming time longer, is difficult to popularize to production reality.

Disclosure of Invention

The utility model discloses an overcome among the prior art that the operating cost who carries out the bacterial colony mixing in the pipette is higher and consuming time longer, is difficult to popularize to the technical problem of production reality, provides a pipette that supplementary bacterial colony mixes, and this pipette can realize the mixing of bacterial colony in the pipette conveniently high-efficiently, need not additional auxiliary assembly, and the operating cost of bacterial colony mixing is lower and can promote to the production reality.

In order to achieve the above purpose, the present invention adopts the following technical solution.

The utility model provides a pipette of supplementary bacterial colony mixture, including first body, the one end of first body is equipped with first liquid mouth of getting, the cover is equipped with the second body in the first body, the one end that first liquid mouth of getting is kept away from to the second body is used for connecting the aurilave, the other end of second body is equipped with the second and gets the liquid mouth, the open end of first liquid mouth of getting and the open end looks parallel and level of second liquid mouth of getting, the outer wall of second body constitutes first cavity with the inner wall of first body, the inside of second body is the second cavity, be equipped with the overflow punch combination of intercommunication first cavity and second cavity on the second body. As described in the background art, the prior pipette capable of mixing bacterial colonies in a tube requires the assistance of external equipment, and has high operation complexity and operation cost, in the utility model, the pipette comprises an outer first tube and an inner second tube, a first chamber is arranged between the first tube and the second tube, a second chamber is arranged in the second tube, a spillway hole group for communicating the first chamber and the second chamber is arranged on the second tube, because the tube diameter of the second tube is far smaller than that of the first tube, and an ear washing ball is connected on the second tube, when the liquid is taken by the pipette, the liquid level in the second tube rises before the liquid level in the first tube rises, when the liquid level in the second tube flows through the spillway hole group, the liquid in the second chamber flows into the first chamber through the spillway hole group, the flow direction of the part of the liquid faces to the first liquid taking port, and the flow direction of the liquid sucked in the first chamber under the action of pressure difference is far away from the first liquid taking port, therefore, the two parts of liquid flowing in opposite directions can be fully mixed, the liquid level in the first chamber is flush with the liquid level in the second chamber after a period of time, at the moment, the liquid taking is finished, as the liquid amount in the second chamber is far smaller than that in the first chamber, the liquid in the first chamber is intermittently and reversely mixed in the completed bacterial colony for uniform mixing, and when the liquid is discharged by the pipette, the liquid in the second chamber is mixed with the liquid in the first chamber, so that the bacterial colony in the two chambers can be uniformly mixed. The pipette can realize the mixing of bacterial colony in the pipette conveniently high-efficiently, need not additional auxiliary assembly, the operating cost of bacterial colony mixing is lower and can promote to production reality.

Preferably, the second pipe is arranged coaxially with the first pipe, and the length of the second pipe is greater than that of the first pipe. The purpose is to facilitate the external ear washing ball of the second tube body.

Preferably, the first liquid extraction port and the second liquid extraction port have a gap in the radial direction of the first pipe. The purpose of arranging the clearance has two, and one of them is that the liquid that makes under the pressure differential effect in being inhaled first cavity with from the liquid reverse disturbance that flows out in the overflow hole group to realize the abundant mixing of bacterial colony, and it is second to avoid liquid level in the second body to rise at the excessive speed, leads to the liquid in the second body to exceed the upper limit of scale mark, causes and gets liquid failure.

Preferably, the outer wall surface of the first pipe body is provided with scale marks arranged along the length direction of the first pipe body.

Preferably, the overflow hole group is close to the second liquid taking port, the overflow hole group comprises a plurality of groups of circumferential overflow hole groups which are arranged at equal intervals along the length direction of the second pipe body, and the circumferential overflow hole groups comprise a plurality of overflow holes which are arranged at equal included angles along the circumferential direction of the second pipe body. The purpose of arranging a plurality of overflow holes in circumference is the convection current volume of increase liquid in the second cavity to guarantee that the bacterial colony in the first cavity can fully mix.

Preferably, the number of the circumferential overflow hole groups is N, and the circumferential overflow hole groups are a 1 st circumferential overflow hole group, a 2 nd circumferential overflow hole group, … …, and an nth circumferential overflow hole group in this order in a direction away from the second liquid-taking port, the 1 st circumferential overflow hole group being located on a side of the scale line closer to the first liquid-taking port. The purpose is to ensure that the liquid taking tube can realize the in-tube uniform mixing of bacterial colonies when the liquid amount corresponding to the minimum scale is taken.

Preferably, the hammer-shaped first liquid taking port comprises a large end and a small end, the large end is connected with the first pipe body, and the second pipe body is in a straight pipe shape.

To sum up, the utility model discloses following beneficial effect has: this pipette can realize the mixing of bacterial colony in the pipette convenient high-efficient, need not additional auxiliary assembly, and the operating cost of bacterial colony mixing is lower and can promote to the production reality.

Drawings

Fig. 1 is a schematic view of the present invention.

Fig. 2 is a different label of the same schematic as fig. 1.

Fig. 3 is a partially enlarged view of a position a in fig. 1.

In the figure:

the liquid level measuring device comprises a first pipe body 1, a first liquid taking port 2, a second pipe body 3, a second liquid taking port 4, a first cavity 5, a second cavity 6, a liquid overflowing hole group 7, a gap 8 and scale marks 9.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present invention, and should not be construed as limiting the present invention.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience of description and for simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example (b):

as shown in fig. 1 to 3, a pipette for assisting bacterial colony mixing comprises a first tube 1, one end of the first tube is provided with a first liquid taking port 2, a second tube 3 is sleeved in the first tube, one end of the second tube, which is far away from the first liquid taking port, is used for connecting an aurilave, the other end of the second tube is provided with a second liquid taking port 4, the open end of the first liquid taking port is flush with the open end of the second liquid taking port, the outer wall of the second tube and the inner wall of the first tube form a first chamber 5, a second chamber 6 is arranged in the second tube, and the second tube is provided with a liquid overflow hole group 7 for communicating the first chamber and the second chamber; the second pipe body is coaxially arranged with the first pipe body, and the length of the second pipe body is greater than that of the first pipe body; a gap 8 exists between the first liquid taking port and the second liquid taking port along the radial direction of the first pipe body; the outer wall surface of the first pipe body is provided with scale marks 9 arranged along the length direction of the first pipe body; the overflow hole group is close to the second liquid taking port, the overflow hole group comprises a plurality of groups of circumferential overflow hole groups which are arranged at equal intervals along the length direction of the second pipe body, and the circumferential overflow hole groups comprise a plurality of overflow holes which are arranged at equal included angles along the circumferential direction of the second pipe body; the number of the circumferential overflow hole groups is N, the circumferential overflow hole groups are a 1 st circumferential overflow hole group, a 2 nd circumferential overflow hole group, … … and an Nth circumferential overflow hole group in sequence towards the direction far away from the second liquid taking port, and the 1 st circumferential overflow hole group is positioned on one side of the scale mark close to the first liquid taking port; the first hammer-shaped liquid taking port comprises a large end and a small end, the large end is connected with the first pipe body, and the second pipe body is in a straight pipe shape.

As shown in fig. 1 and fig. 2, the pipette for assisting bacterial colony mixing includes, from outside to inside, a first tube and a second tube, an upper end of the second tube protrudes out of an upper end surface of the first tube, an upper end of the first tube is a sealed end, a lower end of the first tube is connected to a first liquid taking port, a lower end of the second tube is connected to a second liquid taking port, the first liquid taking port is conical, a large end of the first liquid taking port is connected to a lower end of the first tube, the second tube is straight tube, in this embodiment, the second liquid taking port is also straight tube, so that no obvious distinction is made in the figure, as long as an installation position is known, the second tube is provided with an overflow hole group, in this embodiment, a cross-sectional view of the second tube is given, it can be seen that the overflow holes are symmetrically arranged along a central axis of the second tube, the overflow hole group includes a plurality of circumferential overflow hole groups arranged at intervals along a length direction of the second tube, the circumferential overflow hole group comprises a plurality of overflow holes which are arranged at equal included angles, and a gap exists between the first liquid taking port and the second liquid taking port.

When liquid is taken, the ear washing ball is held to exhaust, the open end of the first liquid taking port is immersed below the liquid level, the open end of the second liquid taking port is flush with the open end of the first liquid taking port, so that the open end of the second liquid taking port is immersed below the liquid level synchronously, the ear washing ball is loosened, the liquid moves upwards along the first chamber and the second chamber, the liquid level in the second chamber is higher than the liquid level in the first chamber due to the small gap between the first liquid taking port and the second liquid taking port and the small pipe diameter of the second pipe body, when the liquid in the second chamber flows through the overflow hole, the liquid in the second chamber flows into the first chamber and is in convection with the liquid in the first chamber, so that the bacterial colonies are fully mixed, and meanwhile, the liquid discharge amount of the overflow hole is smaller than the liquid suction amount in the second chamber, so that the liquid column in the second chamber is still higher than the liquid column in the first chamber, when the liquid column in the second chamber does not rise any more, the liquid column in the second chamber gradually descends, when the heights of the liquid columns in the second chamber and the first chamber are the same, liquid taking is completed, and in the process, the opening end of the first liquid taking port is required to be always positioned below the liquid level; because the liquid amount in the second chamber is far less than the liquid amount in the first chamber, and the liquid in the first chamber is mixed in the completed bacterial colony in an intermittent reverse fluid manner, when the liquid is discharged by the pipette, the liquid in the second chamber is mixed with the liquid in the first chamber, and therefore the bacterial colony mixing in the two chambers can be realized. The pipette can realize the mixing of bacterial colony in the pipette conveniently high-efficiently, need not additional auxiliary assembly, the operating cost of bacterial colony mixing is lower and can promote to production reality.

Claims (7)

1. The utility model provides a pipette of supplementary bacterial colony mixture, a serial communication port, including first body, the one end of first body is equipped with first liquid mouth of getting, the cover is equipped with the second body in the first body, the one end that first liquid mouth of getting is kept away from to the second body is used for connecting the aurilave, the other end of second body is equipped with the second and gets the liquid mouth, the open end of first liquid mouth of getting and the open end looks parallel and level of second liquid mouth of getting, the outer wall of second body constitutes first cavity with the inner wall of first body, the inside of second body is the second cavity, be equipped with the overflow punch combination that communicates first cavity and second cavity on the second body.

2. The pipette of claim 1 wherein the second tube is disposed coaxially with the first tube, the second tube having a length greater than the length of the first tube.

3. The pipette for assisting colony mixing according to claim 1, wherein the first liquid taking port and the second liquid taking port have a gap in a radial direction of the first pipe body.

4. The pipette for assisting colony mixing according to claim 1, wherein the outer wall surface of the first tube body is provided with scale marks arranged along the length direction of the first tube body.

5. The pipette for assisting colony mixing according to claim 4, wherein the overflow hole set is close to the second liquid taking port, the overflow hole set comprises a plurality of circumferential overflow hole sets arranged at equal intervals along the length direction of the second pipe body, and the circumferential overflow hole set comprises a plurality of overflow holes arranged at equal included angles along the circumference of the second pipe body.

6. The pipette for assisting colony mixing according to claim 5, wherein the number of the circumferential overflow hole groups is N, and the circumferential overflow hole groups are a 1 st circumferential overflow hole group, a 2 nd circumferential overflow hole group, … … and an Nth circumferential overflow hole group in sequence in the direction away from the second liquid taking port, and the 1 st circumferential overflow hole group is positioned on the side of the scale mark close to the first liquid taking port.

7. The pipette for assisting colony mixing according to claim 1, 2, 3 or 4, wherein the first liquid taking port in the shape of a hammer comprises a large end and a small end, the large end is connected with the first pipe body, and the second pipe body is in the shape of a straight pipe.

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