CN212321627U

CN212321627U - Novel antibody is hatched device

Info

Publication number: CN212321627U
Application number: CN202021313536.6U
Authority: CN
Inventors: 陈昶舟; 赵海潮
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-07-07
Filing date: 2020-07-07
Publication date: 2021-01-08
Anticipated expiration: 2030-07-07

Abstract

The utility model belongs to the technical field of reaction kits, in particular to a novel antibody incubation device, which comprises a box cover, a box body and a wax block, wherein the box cover is connected with the upper end of the box body through screw threads, the wax block is arranged in the box body, and the diameter of the wax block is 0.3-0.7cm smaller than the inner diameter of the box body; the utility model has the advantages of simple overall structure, convenient operation, the antibody demand is few, incubates efficiently, repeatedly usable for the experiment is high-efficient, go on smoothly providing the guarantee.

Description

Novel antibody is hatched device

Technical Field

The utility model belongs to the technical field of the antibody is hatched, concretely relates to novel antibody is hatched device.

Background

When antibody incubation is performed in an experiment, the traditional antibody incubation method has a plurality of problems:

(1) placing the membrane and the antibody in a small box, and incubating in a shaking table, wherein the method needs a large amount of antibody each time, is difficult to incubate multiple antibodies at the same time, and is incomplete in antibody recovery;

(2) the membrane and the antibody are placed in a small bag made of a disposable plastic packaging membrane, the plastic packaging membrane can be sealed only by using a sealing machine, but the plastic packaging membrane is wetted by liquid and is difficult to be sealed by the sealing machine, so that the antibody diluent is easy to leak out of the plastic packaging membrane, and the antibody waste is caused; secondly, air bubbles in the plastic film cannot be sufficiently excluded when the antibody incubation is performed, and the presence of air bubbles makes the antibody not sufficiently contact with the film, thereby affecting the experimental results. The method causes great waste of time and antibody in sample adding and antibody recovery, and causes resource waste and environmental pollution due to the use of a disposable plastic packaging film;

(3) when carrying out the antibody and hatching, often need carry out the shading operation, but lack supporting shading instrument, need materials such as laboratory staff with tinfoil to carry out the parcel operation, make the antibody landing easily, cause the waste of antibody, and the parcel operation is wasted time and energy, and the operation is inconvenient.

Therefore, it is necessary to provide an incubation cassette that is simple to operate, stable in effect and cost-effective.

SUMMERY OF THE UTILITY MODEL

The utility model provides a novel antibody is hatched device to above-mentioned problem.

In order to achieve the purpose, the utility model adopts the following technical proposal:

the utility model provides a novel antibody is hatched device, includes lid, box body and wax stone, lid threaded connection is in the upper end of box body, the wax stone sets up in the box body, the diameter ratio of wax stone the internal diameter of box body is little 0.3-0.7 cm.

Further, a shading device is arranged above the box cover and consists of a rotating shaft arranged horizontally and two semicircular shading plates arranged on the rotating shaft, the two semicircular shading plates are symmetrically arranged about the axis of the rotating shaft and can rotate around the rotating shaft, a plug-in column is fixedly connected to the middle of the rotating shaft, and a socket used for placing the plug-in column is arranged in the middle of the upper surface of the box cover.

Still further, the up end of box body is provided with the sealing washer, so that the lid prevents liquid seepage or volatilizees with the box body laminating closely.

Furthermore, a cross-shaped groove is formed in the bottom of the box body.

Still further, be provided with the inlet opening in the side middle part of box body the side bottom of box body is provided with the apopore, the apopore sets up the arbitrary one end at the cross recess, so that the apopore is swift smooth when the water is gone out be provided with No. one hole stopper and No. two hole stoppers on inlet opening and the apopore respectively, No. one hole stopper and No. two hole stoppers are the toper hole stopper.

Furthermore, the diameter of the water inlet hole is smaller than that of the water outlet hole.

Furthermore, a connecting band is fixedly connected to the first hole plug and the second hole plug, and the other end of the connecting band is fixedly connected with the outer surface of the box body.

Furthermore, the box cover is made of transparent plastic materials, and the box body is made of non-transparent plastic materials.

Compared with the prior art, the utility model has the advantages of it is following:

1. the utility model discloses lid and box body threaded connection have prevented that lid and box body drop each other when taking the utility model discloses;

2. the box body of the utility model is provided with the sealing ring, so that the box cover is tightly attached to the box body, and the liquid is prevented from leaking or volatilizing;

3. the utility model has the function of moisturizing the wax block, and provides a moist environment for the antibody;

4. the surface of the wax block in the utility model is smooth, which can prevent the incubated hybridization membrane from being damaged;

5. the utility model discloses well wax stone insoluble water can carry out the antibody on the wax stone surface and hatch the operation, has made things convenient for hatching the recovery of accomplishing the back antibody.

6. The wax block in the utility model can float on a small amount of water, so that the wax block can keep horizontal, the level of a hybridization membrane in the whole antibody incubation process is ensured, and the phenomenon of uneven incubation is prevented;

7. the utility model is provided with the shading device which is inserted on the box cover and is inserted when the shading is needed and is taken down when the shading is not needed, thereby meeting different requirements when incubating antibodies;

8. the utility model is composed of a rotating shaft and two turnable semicircular light shields, which is convenient for experimenters to fold one of the semicircular light shields to observe the incubation condition of the antibody at any time in the hybridization process;

9. the cross-shaped groove is arranged at the bottom of the box body, so that the water storage capacity is increased, excessive water at the bottom of the box can not cause the wax block to incline, the humidity in the box is kept, and the volatilization of the over-dry antibody is prevented;

10. in the utility model, the water outlet hole is arranged at any end of the cross groove, so that the water outlet hole can quickly and smoothly discharge water;

11. the connecting belts are fixedly connected on the hole plugs, so that the hole plugs are prevented from being lost in the practical process;

12. the utility model discloses well lid and box body are the plastics material and make, have made things convenient for the experimenter to wash, have reduced the damage, cause the waste of resource.

Drawings

Fig. 1 is a schematic structural diagram of the present invention;

fig. 2 is a schematic structural view of the box cover of the present invention;

fig. 3 is a schematic structural view of the light shielding device of the present invention;

FIG. 4 is a schematic structural view of the cross-shaped groove of the present invention;

fig. 5 is a schematic structural view of the external thread, the water inlet hole, the water outlet hole, the sealing ring, the first hole plug and the second hole plug of the present invention;

FIG. 6 is a schematic view of the position structure of the wax block of the present invention;

in the drawing, a box cover-1, a box body-2, a wax block-3, a socket-4, a sealing ring-5, a semicircular light screen-6, a rotating shaft-7, a plug column-8, a cross groove-9, a connecting belt-10, a water inlet hole-11, a water outlet hole-12, a first hole plug-13 and a second hole plug-14 are arranged.

Detailed Description

In order to further illustrate the technical solution of the present invention, the present invention is further explained by the following embodiments.

As shown in fig. 1 to 6, a novel antibody incubation device comprises a box cover 1, a box body 2 and a wax block 3, wherein the box cover 1 is in threaded connection with the upper end of the box body 2, the box cover 1 is made of transparent plastic material, the box body 2 is made of non-transparent plastic material, a shading device is arranged above the box cover 1, the shading device comprises a rotating shaft 7 arranged horizontally and two semicircular shading plates 6 arranged on the rotating shaft 7, the two semicircular shading plates 6 are symmetrically arranged about the axis of the rotating shaft 7, the two semicircular shading plates 6 can rotate around the rotating shaft 7, a plug-in post 8 is fixedly connected to the middle of the rotating shaft 7, a socket 4 for placing the plug-in post 8 is arranged in the middle of the upper surface of the box cover 1, a sealing ring 5 is arranged on the upper end surface of the box body 2, so that the box cover 1 and the box body 2 are tightly attached to prevent, the bottom of the box body 2 is provided with a cross groove 9, the middle part of the side surface of the box body 2 is provided with a water inlet 11, the bottom of the side surface of the box body 2 is provided with a water outlet 12, the water outlet 12 is arranged at any end of the cross groove 9, so that the water outlet 12 can rapidly and smoothly discharge water, the diameter of the water inlet 11 is smaller than that of the water outlet 12, the water inlet 11 and the water outlet 12 are respectively provided with a first hole plug 13 and a second hole plug 14, the first hole plug 13 and the second hole plug 14 are both conical hole plugs, the minimum diameter of the first hole plug 13 is equal to that of the water inlet 11, the minimum diameter of the second hole plug 14 is equal to that of the water outlet 12, the maximum diameter of the first hole plug 13 is larger than that of the water inlet 11, and the maximum diameter of the second hole plug 14 is larger than that of the water outlet 12, equal fixedly connected with connecting band 10 on a hole stopper 13 and No. two hole stoppers 14, the other end of connecting band 10 with the surface fixed connection of box body 2, wax stone 3 sets up in the box body 2, the diameter ratio of wax stone 3 the internal diameter of box body 2 is little by 0.3-0.7 cm.

When in use:

1) injecting double-distilled water into a cross groove 9 at the bottom of the box body 2 through a water inlet hole 11 until the double-distilled water overflows the cross groove by 0.1-0.3 cm;

2) placing the wax block 3 at the bottom of the box body 2, checking whether the wax block 3 horizontally floats in the box body 2, and if the wax block 3 is uneven in density and unbalanced in center of gravity, so that the wax block 3 cannot horizontally float in the box body 2, replacing the wax block 3 until the wax block can horizontally float in the box body 2;

3) pumping the double distilled water in the groove 9 through the water outlet hole 12 to prevent the wax block from moving in the operation process;

4) after the hybrid membrane is subjected to membrane conversion and rinsing, one surface containing protein of the hybrid membrane is horizontally paved on a plane of the wax block 3 away from double distilled water upwards;

5) dripping an antibody diluent on the hybrid membrane to soak the hybrid membrane in the antibody diluent;

6) repeating the step 1), injecting water into the bottom of the box body 2 to enable the wax block 3 to be horizontal, so that the level of a hybridization membrane in the whole antibody incubation process is ensured, and the phenomenon of uneven incubation is prevented;

7) the box cover 1 is covered on the box body 2 and locked by screw threads;

8) inserting a shading device on the socket 4, unfolding the two semicircular shading plates 6, and shading and protecting the antibodies in the box body;

9) placing the operated antibody incubation device in an environment of 4 ℃ for hybridization reaction;

10) during the hybridization process, one of the semicircular light shielding plates 6 can be closed to observe the condition of the antibody.

The essential features and advantages of the invention have been shown and described above, it will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, but may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. A novel antibody incubation device is characterized in that: including lid (1), box body (2) and wax stone (3), lid (1) threaded connection be in the upper end of box body (2), wax stone (3) set up in box body (2), the diameter ratio of wax stone (3) the internal diameter of box body (2) is little by 0.3-0.7 cm.

2. A novel antibody incubation device according to claim 1, characterized in that: be provided with shade above lid (1), shade comprises axis of rotation (7) that the level set up and two semi-circular light screen (6) of setting on axis of rotation (7), and two semi-circular light screen (6) set up about axis of rotation (7) axis symmetry, and two semi-circular light screen (6) all can revolute axis of rotation (7) and rotate the middle part fixedly connected with plug column (8) of axis of rotation (7) the upper surface middle part of lid (1) is provided with socket (4) that are used for placing plug column (8).

3. A novel antibody incubation device according to claim 1, characterized in that: the upper end face of the box body (2) is provided with a sealing ring (5) so that the box cover (1) is tightly attached to the box body (2) to prevent liquid leakage or volatilization.

4. A novel antibody incubation device according to claim 1, characterized in that: the bottom of the box body (2) is provided with a cross-shaped groove (9).

5. A novel antibody incubation device according to claim 1, characterized in that: the side middle part of box body (2) is provided with inlet opening (11) the side bottom of box body (2) is provided with apopore (12), apopore (12) set up the arbitrary one end in cross recess (9), so that apopore (12) are swift smooth when going out water be provided with No. one hole stopper (13) and No. two hole stoppers (14) on inlet opening (11) and apopore (12) respectively, No. one hole stopper (13) and No. two hole stoppers (14) are the toper hole stopper.

6. A novel antibody incubation device according to claim 5, characterized in that: the diameter of the water inlet hole (11) is smaller than that of the water outlet hole (12).

7. A novel antibody incubation device according to claim 5, characterized in that: equal fixedly connected with connecting band (10) on a hole stopper (13) and No. two hole stoppers (14), the other end of connecting band (10) with the surface fixed connection of box body (2).

8. A novel antibody incubation device according to claim 1, characterized in that: the box cover (1) is made of transparent plastic materials, and the box body (2) is made of non-transparent plastic materials.