CN212321237U

CN212321237U - Cell nucleus staining device

Info

Publication number: CN212321237U
Application number: CN202021041932.8U
Authority: CN
Inventors: 宋驰; 张宇晨; 肖湘月; 冀宏; 丁燕; 蔡小龙; 陈业虎
Original assignee: Changshu Institute of Technology
Current assignee: Changshu Institute of Technology
Priority date: 2020-06-09
Filing date: 2020-06-09
Publication date: 2021-01-08
Anticipated expiration: 2030-06-09

Abstract

A device for cell nucleus dyeing belongs to the technical field of cell nucleus dyeing equipment. The box comprises a box body, wherein a placing chamber is formed at intervals in the length direction of the box body, and the bottom of the placing chamber is hollowed out; the plug board is in plug fit with the lower part of the box body in the length direction and is in sealing fit with the bottom of the placing chamber or is released from the sealing fit; a cover glass supporting mechanism located within the placing chamber; and one side of the box body cover in the length direction is hinged with the upper edge of the rear side of the box body, and the other side of the box body cover is locked or unlocked with the upper part of the front side of the box body. The dyeing method is beneficial to fully contacting the cell nucleus with various dropwise added reagents in the cell nucleus dyeing process so as to improve the dyeing effect; operation links are reduced, and convenience of operation is reflected; the economy and operability are embodied; reduce the probability of contamination of cell nuclei and improve staining efficiency.

Description

Cell nucleus staining device

Technical Field

The utility model belongs to the technical field of the cell nucleus dyeing equipment, concretely relates to device is used in cell nucleus dyeing.

Background

Cell nucleus staining is an important technical means for observing the physiological state of cells, and factors such as avoiding the waste of staining solution and reducing the operation steps in the staining process are generally related to the structure of a cell nucleus beam staining device. However, the cell nucleus staining apparatus in the prior art has the following disadvantages: the consumption of the dyeing liquid is large; the operation steps of the cell nucleus dyeing process are complicated, and the reagent needs to be repeatedly dripped into the dyeing container, so that the convenience in adding the dyeing solution cannot be reflected in the process of switching different steps; the staining solution added to the cell nucleus beam color has a high probability of contamination and relatively low cell nucleus staining efficiency. Based on the foregoing, there is a need for reasonable improvements, and the solutions described below are made in this context.

Disclosure of Invention

The task of the utility model is to provide a help making the cell nucleus fully contact with the various reagents of dropwise add and can improve dyeing effect, be favorable to reducing and external contact and can avoid polluting, be of value to saving the reagent quantity and improving dyeing efficiency and can embody good economic nature and maneuverability's device for cell nucleus dyeing to the in-process of cell nucleus dyeing.

The utility model aims to realize the task that the device for dyeing the cell nucleus comprises a box body, wherein a placing chamber is formed at intervals in the length direction of the box body, and the bottom of the placing chamber is hollowed; the plug board is in plug fit with the lower part of the box body in the length direction and is in sealing fit with the bottom of the placing chamber or is released from the sealing fit; a cover glass supporting mechanism located within the placing chamber; and one side of the box body cover in the length direction is hinged with the upper edge of the rear side of the box body, and the other side of the box body cover is locked or unlocked with the upper part of the front side of the box body.

In a specific embodiment of the present invention, a board sliding fit groove is respectively formed in a lower portion of one side of the box body facing to the length direction, and both sides of the board are in plug-in fit with the board sliding fit groove.

In another specific embodiment of the present invention, a storage chamber gasket groove is formed in the bottom of the box body and around the storage chamber, a storage chamber gasket is embedded in a position corresponding to the storage chamber gasket groove, and the upward side of the insertion plate is in sealing fit with or free of the storage chamber gasket.

In another specific embodiment of the present invention, a fastening cavity is formed on the left end surface of the insertion plate.

In another specific embodiment of the present invention, the box cover is hinged to the rear side of the box body along one side of the length direction of the box body by a hinge member, and the other side of the box cover is locked or unlocked to the upper portion of the front side of the box body by a locking hook.

In yet another specific embodiment of the present invention, a locking hook defining flange is fixed to the upper front side of the case body at a position corresponding to the locking hook, and the locking hook is engaged with or disengaged from the locking hook defining flange; the hinged member is a hinge or a pin shaft.

In a more specific embodiment of the present invention, a locking claw is formed on the locking hook, and a locking claw fitting groove is formed on the locking hook defining flange, and the locking claw is fitted to or removed from the locking claw fitting groove.

In yet another specific embodiment of the present invention, an inclined surface inclined toward the direction of the placing chamber is formed on the case body and around the upper edge of the placing chamber, and the inclined surface has an inclination angle of 45 °.

In a more specific embodiment of the present invention, the cover glass supporting mechanism includes a first inverted t-shaped plate i and a second inverted t-shaped plate ii, the front and rear ends of the bottom of the first inverted t-shaped plate i are fixed to the opposite sides of the front and rear wall bodies of the placing chamber of the box body, and the left side of the upper portion of the first inverted t-shaped plate i is fixed to the left wall body of the placing chamber; the second inverted T-shaped plate II is located on the right side of the first inverted T-shaped plate I, the front end and the rear end of the bottom of the second inverted T-shaped plate II are fixed with one side of the front wall body and the rear wall body of the placing chamber in opposite directions, the right side of the upper portion of the second inverted T-shaped plate II is fixed with the right wall body of the placing chamber, and the space between the bottom of the first inverted T-shaped plate I and the bottom of the second inverted T-shaped plate II is formed into a liquid placing cavity of the placing chamber.

In yet another specific embodiment of the present invention, the box body is a resin box body.

One of the technical effects of the technical proposal provided by the utility model is that the placing chamber is formed at intervals in the length direction of the box body, and the cover glass supporting mechanism is arranged in the placing chamber, thereby being beneficial to fully contacting the cell nucleus with various dropwise added reagents in the process of dyeing the cell nucleus and improving the dyeing effect; secondly, the operator can directly suck the staining solution or the eluent into the needle tube and then align the needle tube with the placing chamber to add the staining solution or the eluent, and the staining solution or the eluent can be quickly discharged from the placing chamber when the plugboard is drawn out, so that the operation links can be reduced, and the convenience of operation is embodied; thirdly, because the space of the placing chamber is relatively small, the reagent is saved, the operation efficiency is improved, and the economical efficiency and the operability are embodied; fourthly, because the staining and the elution are carried out in the placing room which is close to the placing room, the probability of the cell nucleus being polluted can be effectively reduced, and the staining efficiency can be improved.

Drawings

Fig. 1 is a schematic view of an embodiment of the present invention.

Detailed Description

In order to make the technical essence and advantages of the present invention more clear, the applicant below describes in detail the embodiments, but the description of the embodiments is not a limitation of the present invention, and any equivalent changes made according to the inventive concept, which are only formal and not essential, should be considered as the technical scope of the present invention.

In the following description, all the concepts related to the directions or orientations of up, down, left, right, front and rear are directed to the position state of fig. 1, and thus, should not be interpreted as a limitation to the technical solution provided by the present invention.

Referring to fig. 1, a box body 1 is shown, a placing chamber 11 is formed at intervals in the length direction of the box body 1, and the bottom of the placing chamber 11 is hollowed out; an insertion plate 2 is shown, the insertion plate 2 is inserted into and pulled out of the lower part of the box body 1 in the length direction, and is in sealing fit with or is not in sealing fit with the bottom of the placing chamber 11 (to be described later); a cover glass supporting mechanism 3 is shown, the cover glass supporting mechanism 3 being located in the aforementioned placing chamber 11; a box cover 4 is shown, one side of the length direction of the box cover 4 is hinged with the rear upper edge of the box 1, and the other side of the box cover 4 is locked or unlocked with the front upper part of the box 1.

In the present embodiment, although two storage chambers are formed at intervals in the longitudinal direction of the box body, if the number of the storage chambers is increased to 3 or more, the technical scope of the present invention should be considered. In addition if divide into two rows around placing chamber 11, every row is two to three or more, then still belongs to the utility model discloses a technical connotation scope.

The lower part of the opposite side of the length direction of the box body 1 is respectively provided with an inserting plate sliding matching groove 12, and the two sides of the length direction of the inserting plate 2 are in inserting and pulling matching with the inserting plate sliding matching grooves 12, namely, the inserting plate 2 has a drawer or drawing plate effect for the box body 1.

A placing chamber sealing gasket groove 13 is arranged at the bottom of the box body 1 and on the periphery corresponding to the placing chamber 11, a placing chamber sealing gasket 131 is embedded at the position corresponding to the placing chamber sealing gasket groove 13, and the upward side of the inserting plate 2 is in sealing fit with or is not in sealing fit with the placing chamber sealing gasket 131. Specifically, when the drawer 2 is in the state shown in fig. 1, the bottom of the storage chamber 11 is closed by the upward surface thereof being well fitted to the storage chamber gasket 131, and when the drawer 2 is pulled out (pulled out) to the left, the fitting to the storage chamber gasket 131 is released, and the staining solution or the eluent in the storage chamber 11 is discharged.

Preferably, a locking cavity 21 is formed on the left end surface of the insertion plate 2.

One side of the box body cover 4 in the length direction of the box body 1 is hinged with the rear upper edge of the box body 1 through a hinge 41, and the other side of the box body cover 4 is locked or unlocked with the front upper part of the box body 1 through a locking hook 42.

As shown in fig. 1, a locking hook defining flange 14 is fixed to the upper front side of the case 1 at a position corresponding to the locking hook 42, and the locking hook 42 is engaged with or disengaged from the locking hook defining flange 14; in the present embodiment, the hinge 41 is a hinge, but a pin may be used.

A locking claw 421 is formed on the locking hook 42, a locking claw engagement groove 141 is formed on the locking hook defining flange 14, and the locking claw 421 is engaged with or disengaged from the locking claw engagement groove 141. Specifically, when the locking hook 42 is pulled to separate the locking claw 421 from the locking claw engagement groove 141, the cassette cover 4 may be opened, and vice versa.

Preferably, an inclined surface 15 inclined toward the placing chamber 11 is formed on the case 1 at a circumference around the upper edge of the placing chamber 11, and the inclined surface 15 has an inclination angle of 45 °, so that the cleaning effect of the upper portion of the case 1 can be improved.

Continuing to refer to fig. 1, the cover glass supporting mechanism 3 comprises a first inverted-T-shaped plate I31 and a second inverted-T-shaped plate II 32, the front end and the rear end of the bottom of the first inverted-T-shaped plate I31 are fixed with one opposite side of the front wall body and the rear wall body of the placing chamber 11 of the box body 1, and the left side of the upper part of the first inverted-T-shaped plate I31 is fixed with the left wall body of the placing chamber 11; the second T-shaped plate II 32 is located on the right side of the first T-shaped plate I31, the front end and the rear end of the bottom of the second T-shaped plate II 32 are fixed to one side, opposite to the front wall body and the rear wall body of the placing chamber 11, of the right side of the upper portion of the second T-shaped plate II 32 is fixed to the right wall body of the placing chamber 11, and the space between the bottom of the first T-shaped plate I31 and the bottom of the second T-shaped plate II 32 forms a placing chamber liquid discharging cavity 111. In practical use, a cover glass is respectively placed on the front side and the back side of the first reverse T-shaped plate I31 and the second reverse T-shaped plate II 32. The first T-shaped plate I31 and the second T-shaped plate II 32 are T-shaped plates with cross sections.

In this embodiment, the case 1 is a resin case, that is, a resin material is used to make the case.

In operation, the plate 2 is inserted, i.e. in the position shown in fig. 1, and two cover slips are placed in the same chamber 11 as described above, the sides of the two cover slips having nuclei are placed opposite to each other, and the staining solution is sucked up by a needle or a pipette and added to the two chambers 11 until the cover slips are completely immersed. After dyeing, the inserting plate 2 is drawn out, the dyeing solution is discharged, then the eluent is dripped to wash the cover glass, and the dyeing step can be repeated after the inserting plate 2 is cleaned. After the drying is finished, the inserting plate 2 can be pulled out, the box body cover 4 is opened, the box body can be placed in an oven for drying or natural drying, and after the drying is finished, the cover glass is taken out by using tweezers and placed under a microscope for observing the physiological state of the cells.

Of course, the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, and the changes, modifications, additions or replacements made by those skilled in the art within the scope of the present invention also belong to the protection scope of the present invention.

Claims

1. A device for staining cell nucleuses is characterized by comprising a box body (1), wherein a placing chamber (11) is formed at intervals in the length direction of the box body (1), and the bottom of the placing chamber (11) is hollowed out; the plug board (2) is in plug fit with the lower part of the box body (1) in the length direction, and is in sealing fit with the bottom of the placing chamber (11) or is released from the sealing fit; a cover glass supporting mechanism (3), wherein the cover glass supporting mechanism (3) is positioned in the placing chamber (11); and one side of the box body cover (4) in the length direction is hinged with the upper edge of the rear side of the box body (1), and the other side of the box body cover (4) is locked or unlocked with the upper part of the front side of the box body (1).

2. The device for dyeing cell nucleus according to claim 1, characterized in that the lower part of the opposite side of the box body (1) in the length direction is respectively provided with an inserting plate sliding matching groove (12), and the two sides of the inserting plate (2) in the length direction are inserted into and pulled out of the inserting plate sliding matching groove (12).

3. The device for staining cell nuclei according to claim 1, wherein a chamber gasket groove (13) is formed at the bottom of the case body (1) and around the chamber (11), a chamber gasket (131) is inserted in a position corresponding to the chamber gasket groove (13), and the upward side of the insert plate (2) is in sealing engagement with or out of sealing engagement with the chamber gasket (131).

4. The apparatus for nuclear staining according to claim 3, wherein a hand-clasping chamber (21) is formed on the left end surface of the insert plate (2).

5. The apparatus for nuclear staining according to claim 1, wherein one side of the case cover (4) in the length direction of the case (1) is hinged to the upper edge of the rear side of the case (1) by a hinge (41), and the other side of the case cover (4) is locked to or unlocked from the upper portion of the front side of the case (1) by a locking hook (42).

6. The apparatus for nuclear staining according to claim 5, wherein a locking hook defining flange (14) is fixed to the upper front side of the case body (1) at a position corresponding to the locking hook (42), the locking hook (42) being engaged with or disengaged from the locking hook defining flange (14); the hinged part (41) is a hinge or a pin shaft.

7. The apparatus for cell nucleus staining according to claim 6, wherein a locking claw (421) is formed on the locking hook (42), a locking claw fitting groove (141) is formed on the locking hook defining flange (14), and the locking claw (421) is fitted to or released from the locking claw fitting groove (141).

8. The apparatus for cell nucleus staining according to claim 1, wherein an inclined surface (15) inclined in a direction toward the placing chamber (11) is formed on the case (1) and around an upper edge of the placing chamber (11), and the inclined surface (15) is inclined at an angle of 45 °.

9. The device for staining nuclei according to claim 1, wherein the cover glass supporting mechanism (3) comprises a first n-shaped plate I (31) and a second n-shaped plate II (32), the front and rear ends of the bottom of the first n-shaped plate I (31) are fixed to the opposite sides of the front and rear walls of the placing chamber (11) of the box body (1), and the left side of the upper portion of the first n-shaped plate I (31) is fixed to the left wall of the placing chamber (11); the second T-shaped plate II (32) is located on the right side of the first T-shaped plate I (31), the front end and the rear end of the bottom of the second T-shaped plate II (32) are fixed to the opposite side of the front wall body and the rear wall body of the placing chamber (11), the right side of the upper portion of the second T-shaped plate II (32) is fixed to the right wall body of the placing chamber (11), and the space between the bottom of the first T-shaped plate I (31) and the bottom of the second T-shaped plate II (32) is formed into the placing chamber liquid placing cavity (111).

10. The device for nuclear staining according to any of the claims 1 to 9, characterized in that the cartridge (1) is a resin cartridge.