CN212293603U - Double-channel oxygen supply device and bioreactor - Google Patents
Double-channel oxygen supply device and bioreactor Download PDFInfo
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- CN212293603U CN212293603U CN202020854950.1U CN202020854950U CN212293603U CN 212293603 U CN212293603 U CN 212293603U CN 202020854950 U CN202020854950 U CN 202020854950U CN 212293603 U CN212293603 U CN 212293603U
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Abstract
The utility model provides a double-path oxygen supply device, which belongs to the technical field of biology, and comprises a first air inlet pipeline connected with a common oxygen supply device and a second air inlet pipeline connected with a standby oxygen supply device, wherein the other ends of the two air inlet pipelines are mutually connected and connected with an inlet of the oxygen pipeline, the oxygen pipeline is provided with a pressure sensor, an outlet of the oxygen pipeline is connected with a tank body of a bioreactor, and a mass flow meter is arranged between the outlet of the oxygen pipeline and the pressure sensor; the controller receives the air pressure in the oxygen pipeline detected by the pressure sensor, the opening degree of the mass flow meter and the current flow of the oxygen pipeline, and can judge whether the common oxygen supply device is abnormal or not, if the common oxygen supply device is abnormal, the common oxygen supply device is automatically switched to the standby oxygen supply device to supply oxygen to the bioreactor, so that the oxygen supply is stable and uninterrupted, the gas saturation in the bioreactor tank is kept to be larger than a preset value, and cells can keep normal growth.
Description
Technical Field
The application relates to the technical field of biology, in particular to a double-path oxygen supply device and a bioreactor.
Background
When the bioreactor is in a normal culture state, sterile air or oxygen needs to be continuously input into the deep layer of culture solution in the tank for the cells to breathe; when the cell density is increased, the input air can not normally meet the cell breathing requirement, and oxygen needs to be input to ensure the normal breathing of the cells; if the air supply is interrupted in the middle, the normal growth of the cells is affected and even the cells die.
The air supply system of the bioreactor generally comprises one path of air and one path of oxygen, wherein when the cell density is low, the air is supplied to ensure the normal respiration of the cells, and when the cell density is gradually increased, the oxygen is slowly supplied to ensure the normal respiration of the cells; wherein, the oxygen supply equipment is generally divided into an oxygen bottle and an oxygen generator, the cell culture period is often several days, and the longer time needs to reach about 30 days. Therefore, in the cell culture period, the oxygen cylinder has the problem of limited volume, and the oxygen generator has a certain probability of failure; once the intermediate gas supply is interrupted, it may result in the cell state being affected and even dying.
The oxygen supply of the oxygen generator is difficult to avoid not breaking down in the cell culture period, the oxygen supply of the oxygen bottle is difficult to ensure the air supply endurance problem in a long period, and particularly, the problem of oxygen supply interruption can not be solved in time when workers leave work at night, so that cell death is easily caused, and more serious economic and time losses are brought.
SUMMERY OF THE UTILITY MODEL
The application provides a double-circuit oxygen supply device and a bioreactor, which are used for solving the problems of interruption of oxygen supply, insufficient stability and the like when the bioreactor is used for cell culture, and the problem that the normal growth of cells is influenced by the reduction of the saturation of gas in a tank body of the bioreactor.
In order to achieve the purpose, the following scheme is adopted in the application:
in one aspect, an embodiment of the present application provides a dual oxygen supply apparatus, which is used for a bioreactor, and includes:
the system comprises an oxygen pipeline, a first air inlet pipeline, a second air inlet pipeline and a controller;
a valve V1 is arranged on the first air inlet pipeline, and one end of the first air inlet pipeline is connected with a common oxygen supply device;
a valve V2 is arranged on the second air inlet pipeline, and one end of the second air inlet pipeline is connected with a standby oxygen supply device;
the first air inlet pipeline is connected with the other end of the second air inlet pipeline and is connected with the inlet of the oxygen pipeline;
an outlet of the oxygen pipeline is connected with a tank body of the bioreactor, a pressure sensor and a mass flowmeter are arranged on the oxygen pipeline, and the mass flowmeter is arranged between the pressure sensor and the outlet of the oxygen pipeline;
the pressure sensor, the mass flow meter, the valve V1 and the valve V2 are respectively connected with the controller.
Optionally, the mass flow meter includes:
the flow control valve, the flow sensor and the opening controller;
the flow control valve comprises a gear adjusting position and a signal receiving and sending device, and the signal receiving and sending device is connected with the opening controller;
the flow sensor and the opening controller are respectively connected with the controller.
Optionally, the controller includes:
and the proportional integral derivative integral PID controller is connected with the opening controller.
In one aspect, the embodiment of the present application provides a bioreactor, the bioreactor includes any kind of double-circuit oxygen supply device, the reactor jar body that the embodiment of the present application provided and sets up the gas saturation sensor in the internal portion of reactor jar, the gas saturation sensor is connected with double-circuit oxygen supply device's controller.
Optionally, the bioreactor further comprises an air pipeline, an inlet of the air pipeline is connected with an air supply device, an outlet of the air pipeline is connected with the tank body of the bioreactor, a valve V3 is arranged on the air pipeline, and the valve V3 is connected with a controller of the two-way oxygen supply device.
The beneficial effects brought by the technical scheme provided by the embodiment of the application at least comprise:
the utility model provides a double-circuit oxygen supply device, which comprises a first air inlet pipeline connected with a common oxygen supply device and a second air inlet pipeline connected with a standby oxygen supply device, wherein the other ends of the two air inlet pipelines are mutually connected and connected with an oxygen pipeline inlet, the oxygen pipeline is provided with a pressure sensor, an oxygen pipeline outlet is connected with a bioreactor tank body, and a mass flowmeter is arranged between the oxygen pipeline outlet and the pressure sensor; the controller receives the air pressure in the oxygen pipeline detected by the pressure sensor, the opening degree of the mass flow meter and the current flow of the oxygen pipeline, and can judge whether the common oxygen supply device is abnormal or not, if the common oxygen supply device is abnormal, the common oxygen supply device is switched to the standby oxygen supply device to supply oxygen to the bioreactor, so that the oxygen supply is stable and uninterrupted, and the gas saturation in the bioreactor tank is kept.
Drawings
FIG. 1 is a schematic structural diagram of a dual oxygen supply apparatus provided in an exemplary embodiment of the present application;
FIG. 2 is a schematic structural diagram of a mass flow meter of a dual oxygen supply apparatus according to an exemplary embodiment of the present disclosure;
FIG. 3 is a schematic diagram of a bioreactor configuration provided in an exemplary embodiment of the present application;
FIG. 4 is a flow chart of a dual oxygen supply method according to an exemplary embodiment of the present disclosure;
description of the drawings:
1, an oxygen pipeline; 1.1 a first intake line; 1.2 a second air inlet pipeline;
2 a pressure sensor;
3, a mass flow meter; 3.1 flow sensor; 3.2 flow control valve; 3.3 an opening controller;
4, a controller; 5, a reactor tank body; 6 gas saturation sensor; 7 air lines.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the drawings of the embodiments of the present application. It should be apparent that the described embodiments are only some of the embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the application without making any creative effort, shall fall within the protection scope of the application.
In the description of the present application, it should be noted that if the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are used for indicating the orientation or positional relationship based on the orientation or positional relationship shown in the drawings or the orientation or positional relationship which is usually placed when the product of the application is used, the description is only for convenience and simplicity, and the indication or suggestion that the referred device or element must have a specific orientation, be constructed in a specific orientation and be operated, and thus, should not be construed as limiting the present application. Furthermore, the appearances of the terms "first," "second," and the like in the description herein are only used for distinguishing between similar elements and are not intended to be construed as indicating or implying relative importance.
In the description of the present application, it should also be noted that, unless otherwise explicitly stated or limited, the terms "disposed," "mounted," "connected," and "connected" should be interpreted broadly, for example, "connected" may be a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.
In addition, the technical features mentioned in the different embodiments of the present application described below may be combined with each other as long as they do not conflict with each other.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
Fig. 1 shows a dual oxygen supply apparatus provided in the embodiment of the present application, which is used in a bioreactor, and referring to fig. 1, the apparatus includes:
the system comprises an oxygen pipeline 1, a first air inlet pipeline 1.1, a second air inlet pipeline 1.2 and a controller 4;
a valve V1 is arranged on the first air inlet pipeline 1.1, and one end of the first air inlet pipeline 1.1 is connected with a common oxygen supply device;
a valve V2 is arranged on the second air inlet pipeline 1.2, and one end of the second air inlet pipeline 1.2 is connected with a standby oxygen supply device;
the first air inlet pipeline 1.1 is connected with the other end of the second air inlet pipeline 1.2 and is connected with the inlet of the oxygen pipeline 1;
the outlet of the oxygen pipeline 1 is connected with the tank body of the bioreactor, a pressure sensor 2 and a mass flow meter 3 are arranged on the oxygen pipeline 1, and the mass flow meter 3 is arranged between the pressure sensor 2 and the outlet of the oxygen pipeline 1;
the pressure sensor 2, the mass flow meter 3, the valve V1, and the valve V2 are connected to the controller 4, respectively.
For example, in some embodiments, the common oxygen supply device connected to one end of the first air inlet pipeline 1.1 may be an oxygen cylinder or an oxygen generator; the spare oxygen supply device connected to one end of the second air inlet line 1.2 may be an oxygen cylinder.
For example, in some embodiments, the valves V1 and V2 may be solenoid valves, and the opening and closing of the valves V1 and V2 are controlled by the controller 4.
For example, in some embodiments, the controller 4 may use a Programmable Logic (PLC) controller.
Exemplarily, the above-mentioned two-way oxygen supply device possesses two air inlet pipelines, be connected to conventional oxygen gas supply device and reserve oxygen gas supply device respectively, detect the atmospheric pressure state of oxygen pipeline through pressure sensor, detect the flow state that the oxygen pipeline is close to the gas outlet end through mass flow meter, the controller is according to the information that pressure sensor and mass flow meter monitored, judge whether conventional oxygen gas supply device appears unusually, valve V1 is closed to the unusual appearance, open valve V2 and switch to being supplied oxygen by reserve oxygen gas supply device to bioreactor, report to the police to the oxygen gas supply device unusually commonly used simultaneously.
The oxygen supply device is respectively connected with the common oxygen supply device and the standby oxygen supply device through two air inlet pipelines, and in combination with real-time detection of the pressure sensor and the mass flow meter, the controller generates a control instruction through receiving detection information and sends the control instruction to related equipment, so that the oxygen supply of the bioreactor can be continued and stabilized, the stability of oxygen saturation in the reactor tank is maintained, and the probability of cell culture failure caused by oxygen supply problem is reduced; meanwhile, the standby oxygen supply device and the pipeline are arranged, so that personnel do not need to be arranged to watch at night or manually pay attention to whether the oxygen supply end has a problem or not for a long time; the oxygen supply apparatus is left to the maintenance personnel with sufficient time to handle the problem.
Alternatively, referring to fig. 2, the mass flow meter 3 includes:
a flow sensor 3.1, a flow control valve 3.2 and an opening controller 3.3;
the flow sensor 3.1 and the opening controller 3.3 are respectively connected with the controller;
the flow control valve 3.2 comprises a gear adjusting and signal receiving and sending device which is connected with the opening controller 3.3.
Illustratively, as shown in fig. 2, the flow sensor 3.1 is connected to the controller 4, and sends the detected gas flow in the oxygen pipeline to the controller 4 in real time; the opening controller 3.3 acquires the state information of the flow control valve 3.2 and sends the state information of the flow control valve 3.2 to the controller 4, and the opening controller 3.3 acquires the instruction information of the controller 4 and sends the instruction information to the flow control valve 3.2 to adjust the opening of the mass flowmeter.
The flow sensor in the mass flow meter can detect the gas flow of the current oxygen pipeline, the flow control valve can control the opening of the current metering flow meter, the current opening state is received and sent by the opening controller, and the opening state of the mass flow meter is adjusted by receiving an instruction from the controller, so that the opening state of the mass flow meter can be adjusted in real time to enable oxygen supply to be more stable, and the gas saturation in the tank body of the bioreactor is stable.
Optionally, the controller 4 includes:
and the proportional integral derivative integral PID controller is connected with the opening controller 3.3.
Illustratively, the controller 4 includes a PID controller, and analyzes and calculates information received by the controller 4 through the PID controller to generate a PID parameter, and sends the PID parameter to the opening controller 3.3, and the opening controller 3.3 sends an opening adjustment command to control the opening adjustment of the flow control valve 3.2.
The proportional-integral-derivative-integral PID controller is used for adjusting the opening degree of the flow control valve of the mass flow meter, so that the flow control is more accurate, the requirement for oxygen in the tank body of the bioreactor is met, and the stability of the gas saturation is kept.
Fig. 3 shows that the embodiment of the present application provides a bioreactor, the bioreactor comprises any one of the two-way oxygen supply devices provided by the embodiment of the present application, a reactor tank 5 and a gas saturation sensor 6 arranged inside the reactor tank, and the gas saturation sensor 6 is connected with the controller 4.
For example, as shown in fig. 3, an outlet of an oxygen pipeline 1 of the dual oxygen supply apparatus is connected to a reactor tank 5, a gas saturation sensor 6 is arranged in the reactor tank 5, and a controller 4 can receive monitoring information of the gas saturation sensor 6, so that the dual oxygen supply apparatus is adjusted in time through the gas saturation in the reactor tank.
Optionally, the bioreactor further comprises an air pipeline 7, an inlet of the air pipeline is connected with an air supply device, an outlet of the air pipeline is connected with a tank body of the bioreactor, a valve V3 is arranged on the air pipeline, and a valve V3 is connected with a controller of the two-way oxygen supply device.
The controller can control the first air inlet pipeline valve V1, the second air inlet pipeline valve V2 and the air pipeline valve V3, and the gas is supplied to the bioreactor tank body through controlling the 3 valves, so that the gas saturation in the bioreactor tank body is stable.
The above-mentioned dual-channel oxygen supply device and bioreactor will be further described with reference to fig. 1, 2 and 3 for the specific usage of the dual-channel oxygen supply device provided in the present application:
putting cells to be cultured, a culture solution and the like into a reactor tank body 5, determining the gas saturation required by the cell culture type according to the presetting, closing the reactor tank body 5, and starting cell culture;
it should be noted that the gas saturation refers to the oxygen saturation in the reactor tank, for example, the oxygen proportion in the air is 21%, when the reaction starts, only the air is input into the reactor tank, the oxygen pipeline is closed, the oxygen content in the reactor decreases as the oxygen is continuously consumed as the reaction proceeds, and for the convenience of adjusting and measuring the control parameters, the gas saturation sensor 6 uses the oxygen content of 21% in the air as the gas saturation of 100% in the reactor tank.
The controller 4 controls the valve V3 on the air pipeline 7 to open, air is supplied to the reactor tank 5, oxygen consumed by cells is gradually increased along with the progress of the reaction, only the air supply begins to meet the requirement of cell culture, the gas saturation in the reactor tank 5 is reduced from the initial 100% to a preset value (for example, the gas saturation in the reactor tank is preset to be more than 40%), and thus the gas saturation in the reactor tank 5 is 40%, the controller 4 controls the V1 to open, and the opening of the mass flow meter 3 is adjusted according to the current flow and opening states detected by the pressure sensor 2 and the mass flow meter 3 and the oxygen consumption speed in the reactor tank 5, so that the gas saturation in the reactor tank 5 is kept to be more than 40%.
The pressure sensor 2 and the mass flow meter 3 keep real-time detection, when the current flow is detected to be smaller than the current opening state and the current air pressure in the oxygen pipeline is less than 1bar (unit of air pressure: hectopa), the controller closes the valve V1, opens the valve V2, adjusts the opening of the mass flow meter to keep the saturation degree of the gas in the reactor tank to be more than 40 percent, and carries out fault alarm on the common oxygen pipeline; if the current flow is detected to be smaller than the current opening state and the current oxygen pipeline internal air pressure is larger than or equal to 1bar, the controller does not adjust the valve V1 and/or the valve V2, and only adjusts the opening of the mass flow meter to keep the gas saturation in the reactor tank body to be larger than 40%.
FIG. 4 is a schematic flow chart of a dual oxygen supply method provided in the embodiments of the present application, which is applied to a bioreactor provided in the embodiments of the present application, and the method includes:
wherein, the gas saturation in the bioreactor tank body is obtained by a gas saturation sensor arranged in the bioreactor tank body and is sent to a controller, and a first air inlet pipeline valve V1 is controlled by the controller;
Wherein the current opening state QkAcquiring and sending the data to a controller by an opening controller in the mass flowmeter;
Wherein, the current flow state Q of the oxygen pipelineyAcquiring and sending the data to a controller by a flow sensor in the mass flowmeter;
the current pressure value P of the oxygen pipeline is obtained by a pressure sensor on the oxygen pipeline and is sent to the controller;
wherein, the controller controls the opening Q of the mass flowmeter according to the preset and received informationkAdjusting to keep the gas saturation in the bioreactor tank to be more than or equal to the preset saturation;
notably, the current traffic state Qy< Current opening State QkWhen, the following conditions exist:
one is that the valve V1 or the valve V2 is just opened, oxygen does not fill the whole oxygen pipeline, and the opening degree of the mass flow meter is larger than the current flow rate of the oxygen pipeline, so in this case, the air pressure at the inlet of the oxygen pipeline needs to be detected, if the previous air pressure value P is larger than or equal to 1bar, the air inlet pipeline has no fault, the state of the valve V1 or the valve V2 does not need to be adjusted, and only the oxygen needs to wait for filling the whole oxygen pipeline, and the opening degree of the mass flow meter is adjusted, so that the gas saturation in the bioreactor tank can be kept to be larger than or equal to the preset saturation degree;
the other condition is that the common oxygen supply device fails to supply air normally through the first air inlet pipeline, the air pressure at the inlet of the oxygen pipeline is detected, if the front air pressure value P is less than 1bar, the controller confirms that the common oxygen supply device fails, the common oxygen supply device needs to be switched to the standby oxygen supply device for supplying oxygen, the controller controls the valve V1 to be closed, the valve V2 to be opened, and the opening degree of the mass flowmeter is adjusted to keep the gas saturation in the bioreactor tank body to be more than or equal to the preset saturation degree;
the fault alarm is controlled by the controller, and the controller can give an alarm through alarm equipment connected with the controller or equipment such as a monitoring station and the like.
And repeating the steps to keep the gas saturation in the bioreactor tank body to be more than or equal to the preset saturation.
Optionally, adjusting the opening Q of the mass flow meterkThe method comprises the following steps:
according to a preset PID control rule, based on the current gas saturation in the bioreactor tank and the current flow state Q of the oxygen pipelineyDetermining a current PID parameter;
controlling the opening Q of the flow control valve according to the current PID parameterk。
Illustratively, the PID control rule is obtained by a worker through multiple tests and debugging according to different cell culture requirements, and then is preset in the controller, and when cell culture is carried out, the PID control rule can be obtained according to the current gas saturation in the bioreactor tank and the current flow state Q of the oxygen pipelineyThe PID parameters appropriate for the current adjustment being made are determined.
Can make bioreactor jar internal gas saturation more stable through PID control, and acquire corresponding PID control rule and predetermine in the controller through test and debugging many times, can make originally when actually carrying out cell culture, the simplified operation, standardized flow alleviates staff's work load, improves work efficiency and cell culture's stability.
Optionally, based on current gas saturation and oxygen in the bioreactor tankCurrent flow state Q of the gas lineyDetermining the current PID parameter includes:
according to the time t, the gas saturation degree alpha in the tank body of the bioreactortCalculating the oxygen consumption speed v in the reactor tank at the time tt；
According to the oxygen consumption rate vtCalculating the target flow Q of the oxygen pipeline at the time tt；
According to the target flow QtWith the current flow state Q of the oxygen lineyAnd determining the current PID parameters.
Illustratively, the controller is capable of controlling the rate of consumption v of oxygen in the reactor vessel at time ttCalculating to obtain the oxygen flow rate required by the current oxygen pipeline to meet the oxygen consumption speed of the cells in the current bioreactor tank, wherein the oxygen flow rate is the target flow rate QtAccording to the target flow rate QtWith the current flow state Q of the oxygen lineyCan adjust the opening state Q of the mass flowmeterkIn the process, the target flow is continuously corrected through PID control, so that PID parameters are adjusted, and the current flow state Q is ensuredyMore rapid and accurate approach to target flow Qt；
It is particularly noted that as the progress of cell culture advances, the rate of oxygen consumption by the cells changes, so that the target flow rate QtThe change of the PID parameters along with the time is needed to be continuously updated and corrected to keep the oxygen saturation in the bioreactor tank body at a temperature higher than or equal to a preset value, so that the double-path oxygen supply method provided by the application adopts a PID control mode to continuously correct the target flow QtAnd a better control effect is achieved.
Optionally, based on the current gas saturation in the bioreactor tank and the current flow state Q of the oxygen lineyDetermining the current PID parameter further comprises:
according to the type of cell culture carried out in the bioreactor tank at the time t and the oxygen consumption speed v in the bioreactor tanktPredicting the current reaction stage;
according to the current reaction stage and the oxygen consumption speed v in the bioreactor tank bodytPredicting the oxygen consumption speed v in the bioreactor tank at the t +1 momentt+1；
According to the oxygen consumption speed v in the bioreactor tank bodyt+1And adjusting the current PID parameters.
Illustratively, when the type of cell culture currently performed in the bioreactor tank is a cell, and the culture period is 30 days, the relationship between the oxygen consumption rate v and the culture progress when the cell culture is performed in the bioreactor provided herein is v (t) maq(mat) Where t is the time at which the reaction is carried out, maInitial amount of cultured A cells, q (m)at) Is a function of the oxygen consumption at time t of the culture of an initial amount m of A cells;
at a time from the tenth day of the reaction, the oxygen consumption rate in the reactor was vt10According to the function of the oxygen consumption of the cells A at the current reaction moment, the controller can estimate the reaction process of the cells A and predict the oxygen consumption at the next moment, so that the current PID parameters are adjusted, the gas saturation in the bioreactor tank can be kept to be more than or equal to the preset value, and the condition that the gas saturation is reduced to be below the preset value due to the adjustment delay of the mass flow meter in the reaction stage to influence the normal growth of the cells is avoided.
The two-way oxygen supply method provided by the embodiment of the application can be applied to the bioreactor provided by the embodiment of the application, the gas saturation in the tank body of the bioreactor is monitored in real time from the beginning of the reaction, and when the gas saturation in the tank body of the bioreactor is detected to be smaller than a preset value for the first time, the control valve V1 is opened to supply oxygen to the tank body of the bioreactor; when oxygen supply is carried out, the current opening state Q of the mass flowmeter is monitored in real timekWith the current flow state Q of the oxygen lineyWhen Q isy＜QkWhen the air pressure of the oxygen pipeline is less than 1bar, the controller confirms that the common air supply device is abnormal, closes the valve V1, opens the valve V2 and gives a fault alarm; in other cases, thenThe states of the valves V1 and V2 are not adjusted;
the double-path oxygen supply method provided by the embodiment of the application can automatically adjust the oxygen supply state in the bioreactor, when the common oxygen supply device is abnormal, the common oxygen supply device is automatically switched to the standby oxygen supply device and alarms to remind workers to maintain the oxygen supply equipment, the gas saturation in the bioreactor tank body and the state of the double-path oxygen supply device are monitored in real time, and the gas saturation in the bioreactor tank body is kept stable and is more than or equal to the preset value in a PID control mode, so that cells can normally grow, and the problem of culture failure caused by unstable or interrupted oxygen supply is avoided.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications of this invention are intended to be covered by the scope of the invention as expressed herein.
Claims (5)
1. A dual oxygen supply apparatus for use in a bioreactor, comprising:
the system comprises an oxygen pipeline, a first air inlet pipeline, a second air inlet pipeline and a controller;
a valve V1 is arranged on the first air inlet pipeline, and one end of the first air inlet pipeline is connected with a common oxygen supply device;
a valve V2 is arranged on the second air inlet pipeline, and one end of the second air inlet pipeline is connected with a standby oxygen supply device;
the first air inlet pipeline is connected with the other end of the second air inlet pipeline and is connected with the inlet of the oxygen pipeline;
an outlet of the oxygen pipeline is connected with a tank body of the bioreactor, a pressure sensor and a mass flowmeter are arranged on the oxygen pipeline, and the mass flowmeter is arranged between the pressure sensor and the outlet of the oxygen pipeline;
the pressure sensor, the mass flow meter, the valve V1 and the valve V2 are respectively connected with the controller.
2. The dual oxygen supply apparatus of claim 1, wherein the mass flow meter comprises:
the flow control valve, the flow sensor and the opening controller;
the flow control valve comprises a gear adjusting position and a signal receiving and sending device, and the signal receiving and sending device is connected with the opening controller;
the flow sensor and the opening controller are respectively connected with the controller.
3. The dual oxygen supply apparatus of claim 2, wherein the controller comprises: and the proportional integral derivative integral PID controller is connected with the opening controller.
4. A bioreactor comprising a dual oxygen supply apparatus according to any one of claims 1 to 3, a reactor vessel and a gas saturation sensor disposed within the reactor vessel and connected to a controller of the dual oxygen supply apparatus.
5. The bioreactor of claim 4, further comprising an air line, wherein the air line is provided with a valve V3, the inlet of the air line is connected with an air supply device, the outlet of the air line is connected with the tank body of the bioreactor, and the valve V3 is connected with the controller of the two-way oxygen supply device.
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| CN113699039A (en) * | 2020-05-20 | 2021-11-26 | 天信和(苏州)生物科技有限公司 | Two-way oxygen supply device, bioreactor and method |
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| CN113699039A (en) * | 2020-05-20 | 2021-11-26 | 天信和(苏州)生物科技有限公司 | Two-way oxygen supply device, bioreactor and method |
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