CN212293533U

CN212293533U - Organ perfusion equipment

Info

Publication number: CN212293533U
Application number: CN202021615509.4U
Authority: CN
Inventors: 欧映廷; 周明
Original assignee: Liver Biotechnology Shenzhen Co ltd
Current assignee: Liver Biotechnology Shenzhen Co ltd
Priority date: 2020-08-06
Filing date: 2020-08-06
Publication date: 2021-01-05
Anticipated expiration: 2030-08-06

Abstract

The utility model relates to the technical field of biomedicine, in particular to organ perfusion equipment.A water outlet of a constant-temperature water bath is communicated with a water inlet of a snakelike condenser pipe through a pipeline, and a water outlet of the snakelike condenser pipe is communicated with a constant-temperature water inlet of the constant-temperature water bath through a pipeline; be equipped with the filling bottle in the constant temperature water bath, fill the bottle and communicate with first peristaltic pump, snakelike condenser tube through the pipeline in proper order, the upper end delivery port of the inner tube of snakelike condenser tube is used for through the pipeline with establish the inferior cavity venous catheter or the hepatic portal venous catheter intercommunication of the liver in buchner funnel's upper end, connect the liquid cup to establish in buchner funnel's vertical below. The utility model has the advantages that: the utility model utilizes the constant temperature water bath to connect the snakelike condenser tube to maintain the constant temperature of the perfusate flowing in the pipeline; the peristaltic pump replaces manual propulsion of the perfusate, the flow rate of the perfusate can be stably and accurately controlled, and the perfusion operation can be stably and continuously operated for a long time; the poured enzyme solution can be recycled, and the pouring operation cost is reduced.

Description

Organ perfusion equipment

Technical Field

The utility model relates to a biomedical technical field especially relates to an organ fills and equips.

Background

There are two common methods of cell separation, namely mechanical separation and perfusion. Mechanical separation methods are less used because they compromise cell integrity and activity, and yield is extremely low; currently, cells are isolated primarily by an ex vivo or in situ two-step collagenase perfusion method. The perfusion method mostly depends on manually pushing perfusate, is troublesome, has unstable perfusion speed and low separation and extraction efficiency, and although the extracted cells are better than mechanical separation, the cells cannot achieve ideal effect, cannot keep constant temperature, cannot recover enzyme liquid and cannot treat the liver of a large organ.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the technical problem that an organ fills and equips is provided, solve and fill the in-process and need last manual propulsion perfusate, speed is unstable, fills the problem that is not continuous, solves and fills inefficiency, the problem that the productivity is low.

The utility model provides an above-mentioned technical problem's technical scheme as follows: an organ perfusion device comprises a Buchner funnel, a constant-temperature water bath kettle, a first peristaltic pump, a snakelike condenser pipe and a liquid receiving cup, wherein a constant-temperature water outlet of the constant-temperature water bath kettle is communicated with a bottom water inlet of an outer pipe of the snakelike condenser pipe through a pipeline, and a top water outlet of the outer pipe of the snakelike condenser pipe is communicated with a constant-temperature water inlet of the constant-temperature water bath kettle through a pipeline; be equipped with the filling bottle that is used for storing the perfusate in the constant temperature water bath, filling bottle pass through the pipeline with the input intercommunication of first peristaltic pump, the output of first peristaltic pump pass through the pipeline with the lower extreme water inlet intercommunication of the inner tube of snakelike condenser pipe, the upper end delivery port of the inner tube of snakelike condenser pipe is used for passing through the pipeline and establishes the lower chamber venous duct or the hepatic portal venous duct intercommunication of the liver of buchner funnel's upper end, connect the liquid cup to establish buchner funnel's vertical below is used for collecting buchner funnel bottom exhaust perfusate.

The utility model has the advantages that: the utility model utilizes the constant temperature water bath to connect the snakelike condenser tube to maintain the constant temperature of the perfusate flowing in the pipeline; the peristaltic pump replaces manual propulsion of perfusion fluid, can stably control the flow rate, and is suitable for various flow rate requirements; the flow rate of the perfusate can be stably and accurately controlled, the perfusion operation can be stably and continuously operated for a long time, the influence of human factors in the perfusion process is small, the enzyme solution is recycled, the large liver can be perfused, and the perfusion continuity is maintained.

On the basis of the technical scheme, the utility model discloses can also do following improvement.

Further, still include the bubble remover, the bubble remover is established and is connected on the pipeline of the upper end delivery port of the inner tube of snakelike condenser pipe and liver, the upper end delivery port of the inner tube of snakelike condenser pipe passes through the pipeline intercommunication the water inlet intercommunication of bubble remover, the delivery port of bubble remover pass through the pipeline with the lower vena cava duct or the hepatic portal vein pipe intercommunication of liver.

The beneficial effect of adopting the further scheme is that: the bubble remover can remove bubbles carried in the pipeline, and prevents the bubbles from entering the liver to form air embolism and influence the perfusion effect.

The liquid receiving cup is communicated with the input end of the second peristaltic pump through a pipeline, and the output end of the second peristaltic pump is communicated with the filling bottle through a pipeline.

The beneficial effect of adopting the further scheme is that: through setting up the second peristaltic pump, to carrying back the perfusate to the filling bottle, realize the cyclic utilization of perfusate, practice thrift the cost.

Further, still include the iron stand platform, be equipped with on the iron stand platform and be used for supporting respectively snakelike condenser pipe with the support of bubble remover, snakelike condenser pipe with the bubble remover is hung respectively and is held on the support.

The beneficial effect of adopting the further scheme is that: the arrangement of the iron stand platform can facilitate the arrangement of the snakelike condensation pipe and the bubble remover.

Further, still include and be used for supporting buchner funnel's foot rest, buchner funnel establishes on the foot rest, connect the liquid cup to establish buchner funnel's bottom.

The beneficial effect of adopting the further scheme is that: the setting of foot rest is convenient for buchner funnel's placing.

Further, the foot rest includes the ring body, establishes go up the lower ring body of the vertical below of ring body and be used for fixed connection go up the ring body with the branch of ring body down, it is the annuliform to go up the ring body, the quantity of branch is more than three, more than three branch is evenly established go up the ring body with down between the ring body, just the both ends of branch respectively with go up the ring body with lower ring body fixed connection, buchner funnel shelve with go up on the ring body.

The beneficial effect of adopting the further scheme is that: on table surface was arranged in to the lower ring body, supported branch and the last ring body, the internal diameter of going up the ring body was less than buchner funnel upper portion's external diameter, when preventing buchner funnel, passed down buchner funnel's bottom and stretched out the ring body, goes up the ring body and supports with buchner funnel's upper portion outer wall, will connect the liquid cup to arrange down that the ring body is inboard and be located buchner funnel's the vertical below of bottom export and be used for collecting the perfusate.

Further, the pipelines are all silicone tubes.

The beneficial effect of adopting the further scheme is that: the pipelines are all silica gel tubes which are soft tubes, so that the dissolved substances are few and harmless, the components can be conveniently assembled and placed, and the high-pressure steam sterilization can be repeatedly carried out.

Drawings

FIG. 1 is a schematic structural view of the present invention, in which arrows indicate the flow direction of liquid during use;

in the drawings, the components represented by the respective reference numerals are listed below:

1. buchner funnel, 2, constant temperature water bath, 3, first peristaltic pump, 4, snakelike condenser tube, 5, connect the liquid cup, 6, bubble remover, 7, second peristaltic pump, 8, iron stand platform, 9, foot rest, 91, go up the ring body, 92, lower ring body, 93, branch, 10, fill the bottle.

Detailed Description

The principles and features of the present invention are described below in conjunction with the following drawings, the examples given are only intended to illustrate the present invention and are not intended to limit the scope of the present invention.

As shown in FIG. 1, the embodiment of the present invention includes Buchner funnel 1 (Buchner funnel 1 is a ceramic instrument used in laboratory, and also made of plastic, and is used for filtering by vacuum or negative pressure suction, and is shaped as flat cylinder, many small holes are opened on the bottom surface of the cylinder, and a long and narrow cylinder outlet is connected below the small holes), thermostatic water bath 2, first peristaltic pump 3, serpentine condenser tube 4 (the serpentine condenser tube 4 includes an outer tube and an inner tube arranged in the outer tube, the outer tube is provided with a water inlet and a water outlet which are communicated with the inside of the outer tube, the inner tube is spirally arranged in the outer tube, and the two ends of the inner tube are respectively extended out of the outer tube) and liquid receiving cup 5. in this embodiment, the thermostatic water bath 2 adopts super thermostatic water bath 2 (super thermostatic water bath 2 is constructed by a metal plate shell, a control box is directly mounted on the water tank, and two thermostatic water inlet and outlet nozzles are arranged beside the thermostatic water pipe, and a high quality water pump is The structure solves the defect of uneven warm water, and ensures that the temperature control precision and the water uniformity of the instrument have higher quality. ) A constant-temperature water outlet of the constant-temperature water bath 2 is communicated with a bottom water inlet of an outer pipe of the snake-shaped condensation pipe 4 through a pipeline, and a top water outlet of the outer pipe of the snake-shaped condensation pipe 4 is communicated with a constant-temperature water inlet of the constant-temperature water bath 2 through a pipeline; be equipped with the filling bottle that is used for storing the perfusate in the constant temperature water bath 2, filling bottle pass through the pipeline with the input intercommunication of first peristaltic pump 3, the output of first peristaltic pump 3 pass through the pipeline with the lower extreme water inlet intercommunication of the inner tube of snakelike condenser tube 4, the upper end delivery port of the inner tube of snakelike condenser tube 4 is used for passing through the pipeline and establishes the inferior chamber venous duct or the hepatoportal venous duct intercommunication of the liver of buchner funnel 1, connect liquid cup 5 to establish buchner funnel 1's vertical below is used for collecting 1 bottom exhaust perfusate of buchner funnel, in this embodiment, the pipeline preferentially adopts the silicone tube, and the pipeline all adopts the silicone tube, and the silicone tube is soft pipe, and the equipment of being convenient for between the part is placed, dissolves out the thing fewly, and harmless to can relapse high-pressure steam sterilization.

The constant temperature water bath 2 is connected with the snakelike condenser tube 4 to maintain the constant temperature of the perfusate flowing in the pipeline; the peristaltic pump replaces manual propulsion of perfusion fluid, can stably control the flow rate, and is suitable for various flow rate requirements; the flow rate of the perfusate can be stably and accurately controlled, the perfusion operation can be stably and continuously operated for a long time, the influence of human factors in the perfusion process is small, the enzyme solution is recycled, the large liver can be perfused, and the perfusion continuity is maintained.

Preferably, the Bubble remover comprises a Bubble remover 6 (Bubble removing device 6) for removing bubbles existing in the liquid, wherein the Bubble remover 6 is provided with a conical inner cavity, a liquid inlet pipe, a liquid outlet pipe and an exhaust pipe, the liquid inlet pipe is connected with the large end of the conical inner cavity in a tangential direction, the small end of the conical inner cavity of the liquid outlet pipe is connected, the inlet of the exhaust pipe is positioned at the inner side of the conical top end in the cavity, the outlet of the exhaust pipe is communicated with the atmosphere, when pressurized liquid is input in a tangential direction, namely, the pressurized liquid flows in the conical cavity in a rotating manner, centrifugal separation occurs between gas and liquid, the gas is gathered towards the center and causes axial gas pressure difference along with the increase of the flow rate of the liquid, so that the gas flows towards the conical top and is exhausted from the exhaust pipe, and quick degassing is realized), the Bubble remover 6 is arranged on a pipeline connecting the upper end water outlet of the inner pipe of the snakelike condensation pipe 4 and the liver, and the upper end water outlet of the inner pipe of The water outlet of the bubble remover 6 is communicated with the lower cavity venous duct or the hepatic portal venous duct of the liver through a pipeline, and the bubble remover 6 can remove bubbles carried in the pipeline and prevent the bubbles from entering the liver to form air embolism to influence the perfusion effect.

The utility model discloses an in the embodiment, still include second peristaltic pump 7, connect liquid cup 5 through the pipeline with the input intercommunication of second peristaltic pump 7, the output of second peristaltic pump 7 pass through the pipeline with fill the bottle intercommunication, through setting up second peristaltic pump 7, to carrying back the perfusate to filling the bottle, realize the cyclic utilization of perfusate, practice thrift the cost.

Need explain, still include iron stand platform 8, be equipped with on the iron stand platform 8 and be used for supporting respectively snakelike condenser pipe 4 with the support of bubble remover 6, snakelike condenser pipe 4 with bubble remover 6 is hung respectively and is held on the support, the setting of snakelike condenser pipe 4 and bubble remover 6 of being convenient for of iron stand platform 8's setting.

The embodiment of the utility model discloses an embodiment is still including being used for supporting buchner funnel 1's foot rest 9, buchner funnel 1 establishes on the foot rest 9, connect liquid cup 5 to establish buchner funnel 1's bottom, foot rest 9's setting is convenient for placing of buchner funnel 1. Specifically, foot rest 9 includes ring body 91, establishes go up ring body 92 and be used for fixed connection down of the vertical below of ring body 91 go up ring body 91 with the branch 93 of ring body 92 down, it is the annuliform to go up ring body 91, the quantity of branch 93 is more than three, more than three branch 93 evenly establishes go up ring body 91 with down between the ring body 92, just the both ends of branch 93 respectively with go up ring body 91 with ring body 92 fixed connection down, buchner funnel 1 shelve with go up on ring body 91. Lower ring body 92 is arranged on table surface, supports branch 93 and upper ring body 91, and the internal diameter of upper ring body 91 is less than buchner funnel 1 upper portion's external diameter, when preventing buchner funnel 1, passes buchner funnel 1's bottom downwards and stretches out upper ring body 91, and upper ring body 91 supports with buchner funnel 1's upper portion outer wall, will connect liquid cup 5 to arrange in the vertical below that the bottom export of lower ring body 92 is inboard and lie in buchner funnel 1 is used for collecting the perfusate.

The working principle is as follows:

1. the water outlet of the super constant temperature water bath 2 is connected with the water inlet at the lower end of the outer pipe of the snakelike condensation pipe 4 by the silica gel pipe, the water outlet at the upper end of the outer pipe of the snakelike condensation pipe 4 is connected with the water inlet of the super constant temperature water bath 2, the temperature is set, and the water circulation is opened.

2. The bottle filled with the perfusate is placed in a super constant-temperature water bath 2 for preheating, a silicon rubber tube is used for connecting a water inlet at the lower end of an inner tube of the snakelike condenser tube 4 with the perfusate through a peristaltic pump, a water outlet at the upper end of the inner tube of the snakelike condenser tube 4 is connected with a water inlet of the bubble remover 6, and a silicon rubber tube is used for connecting a water outlet at the lower end of the bubble remover 6 with a lower cavity venous tube or a portal venous tube of the liver.

3. The liver was placed in buchner funnel 1, buchner funnel 1 was placed on homemade tripod 9. During perfusion, the silicone tube can be fixed on the blood vessel of the liver for liquid feeding by using hemostatic forceps or suture.

4. The water outlet at the lower end of the Buchner funnel 1 is used for receiving perfusate after passing through the liver by a liquid receiving cup 5, if the perfusate can be reused, the perfusate of the liquid receiving cup 5 is discharged into a bottle filled with the perfusate in the super constant temperature water bath kettle 2 by a silicon hose through a peristaltic pump, and the perfusate is recycled.

5. The device is firstly tested before formal operation, the peristaltic pump is started to fill the whole pipeline system with perfusion liquid, after the condition that the pipeline has no water leakage is checked, the flow speed of the peristaltic pump is regulated to be the lowest after air in the pipeline is exhausted, and then the device is formally connected with the liver to start perfusion operation. Connecting the perfusion system with the insertion tube of the hepatic portal vein, such as no hepatic portal vein insertion tube (insertion tube completed by a doctor), and clamping the connective tissue of the hepatic portal vein by using hemostatic forceps when inserting the connector of the perfusion system to prevent leakage; the bubbles are not generated, and the generated bubbles are eliminated as much as possible. And (3) opening the peristaltic pump and the timer, slowly adjusting the rotating speed to the target rotating speed, observing whether the liver perfusion is normal or not, and whether the inferior vena cava outflow is normal or not, clamping the outlet by using forceps, observing whether the liver is enlarged or not, and if the liver is enlarged, indicating that the perfusion is normal.

The utility model discloses following beneficial effect has:

1. the flow rate of the perfusate can be stably and accurately controlled; 2. the perfusion operation can be stably and continuously operated for a long time; 3. the influence of human factors in the perfusion process is small; 4. the perfusate can be recycled, so that the cost is saved; 4. the application range is wide, and the method is basically suitable for extracting all kinds of liver cells; 5. the perfusion effect is obvious, the liver digestion is full, the cell yield is high, and the vitality is high; 6. the modular construction is realized, all parts which directly contact perfusate and the liver can be directly sterilized by high-temperature high-pressure steam, and other parts can be sterilized by ultraviolet rays, so that the aseptic requirements are met, and the parts can be recycled.

The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.

Claims

1. The organ perfusion equipment is characterized by comprising a Buchner funnel (1), a constant-temperature water bath (2), a first peristaltic pump (3), a snake-shaped condensation pipe (4) and a liquid receiving cup (5), wherein a constant-temperature water outlet of the constant-temperature water bath (2) is communicated with a bottom water inlet of an outer pipe of the snake-shaped condensation pipe (4) through a pipeline, and a top water outlet of the outer pipe of the snake-shaped condensation pipe (4) is communicated with a constant-temperature water inlet of the constant-temperature water bath (2) through a pipeline; be equipped with in constant temperature water bath (2) and be used for storing filling bottle (10) of perfusate, fill bottle (10) through the pipeline with the input intercommunication of first peristaltic pump (3), the output of first peristaltic pump (3) pass through the pipeline with the lower extreme water inlet intercommunication of the inner tube of snakelike condenser pipe (4), the upper end delivery port of the inner tube of snakelike condenser pipe (4) is used for through the pipeline with establish the lower chamber venous catheter or the hepatic portal venous catheter intercommunication of the liver of buchner funnel (1) upper end, connect liquid cup (5) to establish the vertical below of buchner funnel (1) is used for collecting buchner funnel (1) bottom discharge's perfusate.

2. An organ perfusion apparatus according to claim 1, further comprising a bubble remover (6), wherein the bubble remover (6) is provided on a pipeline connecting an upper end water outlet of the inner tube of the serpentine condenser tube (4) and the liver, the upper end water outlet of the inner tube of the serpentine condenser tube (4) is communicated with a water inlet of the bubble remover (6) through a pipeline, and a water outlet of the bubble remover (6) is communicated with a lower vena cava or a portal vein of the liver through a pipeline.

3. An organ perfusion kit according to claim 1, further comprising a second peristaltic pump (7), the drip cup (5) being in communication with an input of the second peristaltic pump (7) via tubing, and an output of the second peristaltic pump (7) being in communication with the perfusion bottle (10) via tubing.

4. An organ perfusion apparatus according to claim 2, further comprising an iron stand (8), wherein the iron stand (8) is provided with a support for supporting the serpentine condenser tube (4) and the bubble remover (6), respectively, and the serpentine condenser tube (4) and the bubble remover (6) are respectively hung on the support.

5. An organ perfusion apparatus according to any one of claims 1 to 4, further comprising a foot rest (9) for supporting the buchner funnel (1), the buchner funnel (1) being provided on the foot rest (9), the liquid-receiving cup (5) being provided at the bottom of the buchner funnel (1).

6. An organ perfusion apparatus according to claim 5, characterized in that the foot rest (9) comprises an upper ring (91), a lower ring (92) arranged vertically below the upper ring (91), and struts (93) for fixedly connecting the upper ring (91) and the lower ring (92), the upper ring (91) is a circular ring, the number of struts (93) is more than three, the struts (93) are uniformly arranged between the upper ring (91) and the lower ring (92), both ends of the struts (93) are fixedly connected with the upper ring (91) and the lower ring (92), respectively, and the buchner funnel (1) rests on the upper ring (91).

7. An organ perfusion apparatus according to any one of claims 1 to 4, wherein the conduits are silicone tubing.