CN212275777U - Enzyme-linked immunoassay kit for detecting olaquindox - Google Patents

Abstract

The utility model discloses an enzyme-linked immunoassay kit for detecting olaquindox, which comprises a plane layer, a built-in layer and a placing layer, wherein the built-in layer is arranged on the placing layer, and the plane layer is arranged on the built-in layer; the built-in layer comprises a filtrate column, a reaction column, an activation column, a crushing tank, a discharge port, a movable door, a coarse liquid pipe, a coarse liquid valve, a limiter, a test tube clamp, a crushing knife, a swing motor, a transmission pipe, a waste liquid tank and a filtrate tank. The utility model belongs to the technical field of food safety, specifically indicate an ELISA kit for detecting olaquindox, improved the utilization efficiency of gained experimental materials, reduced the cost of labor, improved work efficiency, the protection experiment environment makes solution, equipment integration, makes the experiment more swiftly convenient, has effectively solved current kit inefficiency on the existing market, and self structure is complicated, maintains comparatively inconvenient problem.

Description

Enzyme-linked immunoassay kit for detecting olaquindox

Technical Field

The utility model belongs to the technical field of food safety, in particular to an enzyme-linked immunoassay kit for detecting olaquindox.

Background

Olaquindox, also known as quinamide alcohol, is banned as a feed additive in both the united states and the european union because olaquindox has moderate to significant cumulative toxicity, has significant teratogenic effects on most animals, and also has teratogenic, mutagenic, and carcinogenic effects on humans. The use of olaquindox in poultry and aquaculture is prohibited as specified in the pharmacopoeia of the Chinese veterinary pharmacopoeia. At present, some farms in the market add olaquindox as an additive to the feed, so a kit for rapidly detecting whether the feed contains olaquindox is necessary.

SUMMERY OF THE UTILITY MODEL

To the above situation, for overcoming the defects of the prior art, the utility model provides an detect enzyme-linked immunoassay kit of olaquindox has improved the utilization efficiency of gained experimental materials, has reduced the cost of labor, improves work efficiency, and the protection experiment environment makes solution, equipment integration, makes the experiment more swiftly convenient, has effectively solved current kit inefficiency on the existing market, and self structure is complicated, maintains comparatively inconvenient problem.

The utility model adopts the following technical scheme: the utility model relates to an enzyme-linked immunoassay kit for detecting olaquindox, which comprises a plane layer, an internal layer and a placing layer, wherein the internal layer is arranged on the placing layer, and the plane layer is arranged on the internal layer; the planar layer comprises a 5% methanol solution storage pipe orifice, a 40% methanol solution storage pipe orifice, a 0.02mol/L hydrochloric acid solution storage pipe orifice, a 0.01mol/L hydrochloric acid solution storage pipe orifice, an ultrapure water storage pipe orifice, a fan, an SPE small column air drying groove, a crushing button, a crude liquid collection button, a shaking button, a waste liquid discharge button, a filtrate collection button and an air blowing button, wherein the 5% methanol solution storage pipe orifice is arranged on one side edge of the planar layer, the 40% methanol solution storage pipe orifice is arranged on the left side of the 5% methanol solution storage pipe orifice and is close to the side edge of the planar layer, the 0.02mol/L hydrochloric acid solution storage pipe orifice is arranged on the left side of the 40% methanol solution storage pipe orifice and is close to the side edge of the planar layer, the 0.01mol/L hydrochloric acid solution storage pipe orifice is arranged on the left side of the 0.02mol/L hydrochloric acid solution storage pipe orifice and is, the ultrapure water storage pipe mouth of pipe is located 0.01mol/L hydrochloric acid solution storage pipe mouth of pipe left side and is close to the side of plane layer, the opposite side edge on plane layer is located to the fan, smash the button and locate ultrapure water storage pipe mouth of pipe one side and press close to the ultrapure water storage pipe mouth of pipe, thick liquid is collected the button and is located and smash button one side and press close to crushing button, it locates thick liquid and collects button one side and press close to thick liquid and collect the button to rock the button, the side edge that waste liquid discharge button and fan all pressed close to is located to the button is collected to filtrating is collected to the blast air button one side, SPE pillar air drying groove is located fan and filtrating and is collected between.

Further, the built-in layer comprises a filtrate column, a reaction column, an activation column, a crushing tank, a discharge port, a movable door, a crude liquid pipe, a crude liquid valve, a limiter, a test tube clamp, a crushing knife, a swing motor, a transmission pipe, a waste liquid tank and a filtrate tank, the movable door is arranged on the left side wall of the built-in layer, the crushing tank is arranged on the top of the built-in layer and embedded in the built-in layer, the discharge port is arranged on the top of the crushing tank, the crushing knife is arranged in the crushing tank, the crude liquid pipe is arranged at the bottom end of the left side wall of the crushing tank, the crude liquid valve is arranged on the crude liquid pipe, the swing motor is arranged below the crushing tank, the limiter is arranged on the left side of the swing motor, the test tube clamp is arranged on the left side of the limiter, the waste liquid tank is arranged on the bottom wall of the built-in layer, the filtrate tank is arranged on the right, the reaction column is arranged on the right side of the activation column, the filtrate column is arranged on the right side of the reaction column, three transmission pipes are arranged, one end of each transmission pipe is respectively arranged on the bottom ends of the activation column, the reaction column and the filtrate column, the other ends of the transmission pipes below the activation column and the reaction column are connected with the waste liquid tank, and the other end of the transmission pipe below the filtrate column is connected with the filtrate tank.

Further, the placing layer comprises a waste liquid valve, a waste liquid pipe, a filtrate valve, a filtrate pipe, a beaker, a device groove, an SPE small column groove, a spare groove, a filter membrane groove, a spare test tube groove and a culture dish groove, one end of the waste liquid pipe is connected with the bottom end of the waste liquid groove, the other end of the waste liquid pipe is arranged outside the placing layer, the waste liquid valve is arranged at one end of the waste liquid pipe and is close to the waste liquid groove, the other end of the filtrate pipe is arranged outside the placing layer, one end of the filtrate pipe is arranged at the bottom end of the filtrate tank, the filtrate valve is arranged at one end of the filtrate pipe and is close to the filter membrane groove, the beaker is arranged at the left side wall edge of the placing layer, the device groove is arranged at the right side of the beaker and is attached to the outer wall of the placing layer, the filter membrane groove is arranged at the right side of the beaker and is close to the inner side wall of the placing layer, the SPE small, the culture dish groove is arranged on the right side of the standby test tube groove.

Further, smash the button and establish and smash the sword electricity and be connected, the thick liquid is collected the button and is established and be connected with thick liquid valve electricity, it establishes to rock the button and is connected with swing motor electricity, waste liquid discharge button establishes and is connected with the waste liquid valve electricity, filtrating is collected the button and is established and is connected with filtrating valve electricity, the fan electricity is connected is located to the blast air button.

Adopt above-mentioned structure the utility model discloses the beneficial effect who gains as follows: the utility model provides an detect ELISA kit of olaquindox, under the experimental materials that the correspondence outdoor was gathered leads to the material deactivation to influence experimental data's the condition because of the time overlength, adopt the utility model discloses can improve the on-the-spot treatment effeciency of material, handle and preserve the experimental materials rapidly, utilize the function in the kit, can reduce artifical consumeing during experimental verification, can effectively retrieve the waste solution that the experiment produced, play certain help to the environmental protection, make the experiment more convenient, effectively solved the current market on the kit inefficiency, and self structure is complicated, maintain comparatively inconvenient problem.

Drawings

FIG. 1 is a perspective view of an ELISA kit for the detection of olaquindox according to the present invention;

FIG. 2 is a top view of the ELISA kit for detecting olaquindox of the present invention;

FIG. 3 is a side sectional view of an ELISA kit for detecting olaquindox of the present invention;

FIG. 4 is the bottom inside view of the ELISA kit for detecting olaquindox of the present invention.

Wherein, 1, a plane layer, 2, a built-in layer, 3, a placing layer, 4, 5% methanol solution storage pipe orifice, 5, 40% methanol solution storage pipe orifice, 6, 0.02mol/L hydrochloric acid solution storage pipe orifice, 7, 0.01mol/L hydrochloric acid solution storage pipe orifice, 8, ultrapure water storage pipe orifice, 9, a fan, 10, SPE small column air drying groove, 11, crushing button, 12, crude liquid collection button, 13, shaking button, 14, waste liquid discharge button, 15, filtrate collection button, 16, air blowing button, 17, filtrate column, 18, reaction column, 19, activation column, 20, crushing groove, 21, discharge port, 22, movable door, 23, crude liquid pipe, 24, crude liquid valve, 25, limiter, 26, test tube clamp, 27, crushing knife, 28, swing motor, 29, transmission tube, 30, waste liquid groove, 31, filtrate groove, 32, waste liquid valve, 33, waste liquid valve, 32, waste valve, A waste liquid pipe, 34, a filtrate valve, 35, a filtrate pipe, 36, a beaker, 37, an equipment groove, 38, an SPE small column groove, 39, a standby groove, 40, a filter membrane groove, 41, a standby test tube groove, 42 and a culture dish groove.

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments; based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

As shown in fig. 1-4, the enzyme-linked immunoassay kit for detecting olaquindox of the present invention comprises a planar layer 1, a built-in layer 2 and a placing layer 3, wherein the built-in layer 2 is disposed on the placing layer 3, and the planar layer 1 is disposed on the built-in layer 2; plane layer 1 includes 5% methanol solution storage tube mouth of pipe 4, 40% methanol solution storage tube mouth of pipe 5, 0.02mol/L hydrochloric acid solution storage tube mouth of pipe 6, 0.01mol/L hydrochloric acid solution storage tube mouth of pipe 7, ultrapure water storage tube mouth of pipe 8, fan 9, SPE columella air-drying groove 10, smashes button 11, thick liquid collection button 12, rock button 13, waste liquid discharge button 14, filtrating collection button 15 and air- blast button 16, 5% methanol solution storage tube mouth of pipe 4 is located on one side of plane layer 1, 5% methanol solution storage tube mouth of pipe 5 is located on 5% methanol solution storage tube mouth of pipe 4 left side and is close to the side of plane layer 1, 0.02mol/L hydrochloric acid solution storage tube mouth of pipe 6 is located on 40% methanol solution storage tube mouth of pipe 5 left side and is close to the side of plane layer 1, 0.01mol/L hydrochloric acid solution storage tube mouth of pipe 7 is located on 0.02mol/L hydrochloric acid solution storage tube mouth of pipe 6 left side and is close to plane layer Side, ultrapure water storage tube mouth of pipe 8 locates 0.01mol/L hydrochloric acid solution storage tube mouth of pipe 7 left side and is close to the side of plane layer 1, fan 9 is located on the opposite side of plane layer 1, smash button 11 and locate ultrapure water storage tube mouth of pipe 8 one side and press close to ultrapure water storage tube mouth of pipe 8, button 12 is collected to thick liquid locates and smashes button 11 one side and press close to and smash button 11, it locates thick liquid and collects button 12 one side and presses close to thick liquid and collects button 12 to rock button 13 to rock button 14, waste liquid discharges button 14 and locates and rock button 13 one side and rock and press close to button 13, the side edge that waste liquid discharge button 14 and fan 9 all pressed close to is located to filtrate discharge button 15 to filtrating is collected to filtrating button 15, SPE pillar air drying groove 10 is located between fan 9 and filtrating collection button 15.

Further, the built-in layer 2 comprises a filtrate column 17, a reaction column 18, an activation column 19, a crushing tank 20, a discharge port 21, a movable door 22, a crude liquid pipe 23, a crude liquid valve 24, a stopper 25, a test tube clamp 26, a crushing knife 27, a swing motor 28, a transmission pipe 29, a waste liquid tank 30 and a filtrate tank 31, wherein the movable door 22 is arranged on the left side wall of the built-in layer 2, the crushing tank 20 is arranged on the wall top of the built-in layer 2 and embedded in the built-in layer 2, the discharge port 21 is arranged on the top of the crushing tank 20, the crushing knife 27 is arranged in the crushing tank 20, the crude liquid pipe 23 is arranged at the bottom end of the left side wall of the crushing tank 20, the crude liquid valve 24 is arranged on the crude liquid pipe 23, the swing motor 28 is arranged below the crushing tank 20, the stopper 25 is arranged on the left side of the swing motor 28, the test tube clamp 26 is arranged on the left side of the stopper 25, the waste liquid tank 30 is arranged on the bottom wall of the built-in, the activation column 19 is arranged on the right side of the crushing tank 20 and is arranged in the built-in layer 2, the reaction column 18 is arranged on the right side of the activation column 19, the filtrate column 17 is arranged on the right side of the reaction column 18, three transmission pipes 29 are arranged, one end of each transmission pipe 29 is arranged at the bottom end of each activation column 19, the bottom end of each reaction column 18 and the bottom end of each filtrate column 17, the other end of each transmission pipe 29 below the activation column 19 and the reaction column 18 is connected with the waste liquid tank 30, and the other end of each transmission pipe 29 below the filtrate column 17 is connected with the filtrate tank 31.

Further, the placing layer 3 comprises a waste liquid valve 32, a waste liquid pipe 33, a filtrate valve 34, a filtrate pipe 35, a beaker 36, a device groove 37, an SPE small column groove 38, a spare groove 39, a filter membrane groove 40, a spare test tube groove 41 and a culture dish groove 42, one end of the waste liquid pipe 33 is connected with the bottom end of the waste liquid groove 30, the other end of the waste liquid pipe 33 is arranged outside the placing layer 3, the waste liquid valve 32 is arranged on one end of the waste liquid pipe 33 and is close to the waste liquid groove 30, the other end of the filtrate pipe 35 is arranged outside the placing layer 3, one end of the filtrate pipe 35 is arranged on the bottom end of the filtrate groove 31, the filtrate valve 34 is arranged on one end of the filtrate pipe 35 and is close to the filter liquid groove 31, the beaker 36 is arranged on the left side wall of the placing layer 3, the device groove 37 is arranged on the right side of the beaker 36 and is attached to the outer wall of the placing layer 3, the filter membrane groove 40 is arranged on the right side of the beaker 36 and is, the spare groove 39 is arranged at the right side of the SPE small column groove 38, the spare test tube groove 41 is arranged at the right side of the filter membrane groove 40, and the culture dish groove 42 is arranged at the right side of the spare test tube groove 41.

Further, the crushing button 11 is electrically connected with the crushing cutter 27, the crude liquid collecting button 12 is electrically connected with the crude liquid valve 24, the shaking button 13 is electrically connected with the swing motor 28, the waste liquid discharging button 14 is electrically connected with the waste liquid valve 32, the filtrate collecting button 15 is electrically connected with the filtrate valve 34, and the air blowing button 16 is electrically connected with the fan 9.

When the device is used specifically, a user needs to put needed tools and solutions into corresponding positions in a kit in a laboratory, when materials are collected outdoors, the user opens the movable door 22 first, takes out a test tube and puts the test tube into the test tube clamp 26, the upper part of the tube orifice of the test tube is opposite to the outlet of the crude liquid tube 23, the movable door 22 is closed, then the user puts the obtained experimental materials into the discharge hole 21 first, the experimental materials enter the crushing groove 20, then 5% methanol solution is added into the discharge hole 21, the user presses the crushing button 11, the crushing knife 27 rotates to crush the experimental materials, presses the crushing button 11 again, the crushing knife 27 stops rotating, presses the crude liquid collecting button 12, the crude liquid valve 24 is opened, the experimental crude liquid in the crushing groove 20 flows out and falls into the test tube, after a certain volume is taken, the crude liquid collecting button 12 is pressed, the crude liquid valve 24 is closed, the user presses the shaking button 13, and the swing motor 28 rotates, the motor swings in the positive direction and the negative direction to rotate between the limiting devices 25, the test tube is taken out after the solution in the test tube is gradually even, a user takes out a filter membrane to cover the filter membrane on the beaker 36, the solution in the test tube is poured into the beaker 36 to obtain a filtrate, then takes out an SPE small column to be placed into the activation column 19, 2ml of methanol solution and 2ml of ultrapure water are injected into the SPE small column to activate the SPE small column, the generated waste liquid flows into the waste liquid tank 30 along the transmission pipe 29, then takes out the activated SPE small column to be placed into the reaction column 18, 2ml of the filtrate is added into the SPE small column, then 2ml of 0.02mol/L hydrochloric acid, 0.01mol/L hydrochloric acid and 5% formaldehyde solution are respectively used for leaching the SPE small column, after leaching is finished, the user takes out the SPE small column to place the SPE small column in the SPE small column air drying tank 10, the blower button 16 is pressed to dry the SPE small column, take off air-dried SPE columella and put into filtrate column 17, the user uses 2 ml's 40% formaldehyde solution to drench into the SPE columella, filtrating passes through transmission pipe 29 and flows in filtrate groove 31, take out a culture dish and put in the export of filtrate pipe 35, press filtrating collection button 15 again, filtrating valve 34 is opened, the filtrating flows out, draw after a certain amount, press filtrating collection button 15 after, filtrating valve 34 closes, put into culture dish groove 42 well with the culture dish lid again, so that guarantee the material activity, it uses liquid phase spectrometer to detect to take back the laboratory, can press waste liquid discharge button 14 after getting back to the laboratory, waste liquid valve 32 opens, waste liquid discharges totally the back, press waste liquid discharge button 14 again, waste liquid valve 32 closes, it is above that the utility model discloses holistic work flow, repeat this step when using next time can.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

The present invention and the embodiments thereof have been described above, but the description is not limited thereto, and the embodiment shown in the drawings is only one of the embodiments of the present invention, and the actual structure is not limited thereto. In summary, those skilled in the art should understand that they should not be limited to the embodiments described above, and that they can design the similar structure and embodiments without departing from the spirit of the invention.

Claims (4)

1. An enzyme-linked immunoassay kit for detecting olaquindox, which is characterized in that: the device comprises a plane layer, a built-in layer and a placing layer, wherein the built-in layer is arranged on the placing layer, and the plane layer is arranged on the built-in layer; the planar layer comprises a 5% methanol solution storage pipe orifice, a 40% methanol solution storage pipe orifice, a 0.02mol/L hydrochloric acid solution storage pipe orifice, a 0.01mol/L hydrochloric acid solution storage pipe orifice, an ultrapure water storage pipe orifice, a fan, an SPE small column air drying groove, a crushing button, a crude liquid collection button, a shaking button, a waste liquid discharge button, a filtrate collection button and an air blowing button, wherein the 5% methanol solution storage pipe orifice is arranged on one side edge of the planar layer, the 40% methanol solution storage pipe orifice is arranged on the left side of the 5% methanol solution storage pipe orifice and is close to the side edge of the planar layer, the 0.02mol/L hydrochloric acid solution storage pipe orifice is arranged on the left side of the 40% methanol solution storage pipe orifice and is close to the side edge of the planar layer, the 0.01mol/L hydrochloric acid solution storage pipe orifice is arranged on the left side of the 0.02mol/L hydrochloric acid solution storage pipe orifice and is, the ultrapure water storage pipe mouth of pipe is located 0.01mol/L hydrochloric acid solution storage pipe mouth of pipe left side and is close to the side of plane layer, the opposite side edge on plane layer is located to the fan, smash the button and locate ultrapure water storage pipe mouth of pipe one side and press close to the ultrapure water storage pipe mouth of pipe, thick liquid is collected the button and is located and smash button one side and press close to crushing button, it locates thick liquid and collects button one side and press close to thick liquid and collect the button to rock the button, the side edge that waste liquid discharge button and fan all pressed close to is located to the button is collected to filtrating is collected to the blast air button one side, SPE pillar air drying groove is located fan and filtrating and is collected between.

2. The enzyme-linked immunoassay kit for detecting olaquindox of claim 1, wherein: the built-in layer comprises a filtrate column, a reaction column, an activation column, a crushing tank, a discharge hole, a movable door, a coarse liquid pipe, a coarse liquid valve, a limiter, a test tube clamp, a crushing knife, a swing motor, a transmission pipe, a waste liquid tank and a filtrate tank, the movable door is arranged on the left side wall of the built-in layer, the crushing tank is arranged on the top of the built-in layer and embedded in the built-in layer, the discharge hole is arranged on the top of the crushing tank, the crushing knife is arranged in the crushing tank, the coarse liquid pipe is arranged at the bottom end of the left side wall of the crushing tank, the coarse liquid valve is arranged on the coarse liquid pipe, the swing motor is arranged below the crushing tank, the limiter is arranged on the left side of the swing motor, the test tube clamp is arranged on the left side of the limiter, the waste liquid tank is arranged on the bottom wall of the built-in layer, the filtrate tank is arranged on the right side of the waste liquid tank and arranged, the filter liquid column is arranged on the right side of the reaction column, three transmission pipes are arranged, one end of each transmission pipe is arranged at the bottom ends of the activation column, the reaction column and the filter liquid column respectively, the other ends of the transmission pipes below the activation column and the reaction column are connected with the waste liquid tank, and the other end of the transmission pipe below the filter liquid column is connected with the filter liquid tank.

3. The enzyme-linked immunoassay kit for detecting olaquindox of claim 2, wherein: the placing layer comprises a waste liquid valve, a waste liquid pipe, a filtrate valve, a filtrate pipe, a beaker, a device groove, an SPE small column groove, a standby groove, a filter membrane groove, a standby test tube groove and a culture dish groove, one end of the waste liquid pipe is connected with the bottom end of the waste liquid groove, the other end of the waste liquid pipe is arranged outside the placing layer, the waste liquid valve is arranged on one end of the waste liquid pipe and is close to the waste liquid tank, the other end of the filtrate pipe is arranged outside the placing layer, one end of the filtrate pipe is arranged on the bottom end of the filtrate tank, the filtrate valve is arranged on one end of the filtrate pipe and is close to the filtrate tank, the beaker is arranged on the left side wall of the placing layer, the device groove is arranged on the right side of the beaker and is attached to the outer wall of the placing layer, the membrane filtering groove is arranged at the right side of the beaker and is close to the inner side wall of the placing layer, the SPE small column groove is arranged at the right side of the equipment groove, the spare groove is arranged on the right side of the SPE small column groove, the spare test tube groove is arranged on the right side of the filter membrane groove, and the culture dish groove is arranged on the right side of the spare test tube groove.

4. The ELISA kit for detecting olaquindox of claim 3, wherein: smash the button and establish and smash the sword electricity and be connected, thick liquid is collected the button and is established and be connected with thick liquid valve electricity, it establishes to rock the button and is connected with swing motor electricity, waste liquid discharge button establishes and is connected with the waste liquid valve electricity, filtrating is collected the button and is established and is connected with filtrating valve electricity, the fan electricity is connected to the blast air button.

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