CN212260070U

CN212260070U - Special graduated cylinder for scirpus marigolense differentiation and proliferation experiment

Info

Publication number: CN212260070U
Application number: CN202020159843.7U
Authority: CN
Inventors: 张付远; 李丹丹; 宋墩福; 李奕
Original assignee: Jiangxi Environmental Engineering Vocational College
Current assignee: Jiangxi Environmental Engineering Vocational College
Priority date: 2020-01-17
Filing date: 2020-01-17
Publication date: 2021-01-01
Anticipated expiration: 2030-01-17

Abstract

The utility model relates to a special graduated flask of scirpus marigoldianus differentiation proliferation experiment. At present, when the scirpus marigoldianus differentiation and proliferation culture medium is prepared, the adopted solution types are more, so that measurement errors can be caused, and the structure of an experiment is influenced. A special graduated cylinder for scirpus marigoldianus differentiation and proliferation experiments comprises the following components: the measuring cylinder comprises a measuring cylinder body (1) and a bottom flat plate seat (2), a circular baffle (3) is arranged below the measuring cylinder body, the circular baffle and the measuring cylinder body are of an integrated structure, a sliding piston plate (4) is arranged inside the measuring cylinder body, a sealing rubber ring (5) is sleeved on an annular concave surface of the sliding piston plate, a push rod (6) is perpendicularly connected to the circle center position of the bottom of the sliding piston plate, the bottom of the push rod is perpendicularly connected with the circle center of the bottom flat plate seat, a jack (7) is arranged at the circle center position of the circular baffle, and balance holes (8) with the same diameter are formed in two sides of the jack. The utility model discloses be applied to special graduated flask of scirpus marigoldianus differentiation proliferation experiment.

Description

Special graduated cylinder for scirpus marigolense differentiation and proliferation experiment

Technical Field

The utility model relates to a special graduated flask of scirpus marigoldianus differentiation proliferation experiment.

Background

At present, when a scirpus marigoldianus differentiation and proliferation method is tested, a culture medium needs to be prepared, and the preparation of the culture medium comprises the following steps:

(1) the formula of the culture medium is as follows: MS + BA2mg/L + ZT1mg/L + TDZ2mg/L + sugar 30g/L + agar 4.1 g/L.

(2) Preparing a mother solution: the mother liquor is divided into four categories of macroelements, microelements, iron salts and organic substances, and is prepared according to MS table parameters. Preparing mother liquor while preparing 2mg/l of 6-BA, 1mg/l of ZT and 2mg/l of TDZ, and dissolving with small amount of 95% ethanol and 1mol/l of NAOH or HCL respectively. And (5) fixing the volume, and placing the mixture in a refrigerator for later use.

(3) Preparation of a culture medium: 100ml of macroelement mother liquor with the volume of 10 times, 1ml of microelement mother liquor with the volume of 1000 times, 10ml of ferric salt element with the volume of 100 times, 10ml of inositol with the volume of 100 times, 1ml of organic 1000 times, 2ml of 6-BA element mother liquor with the volume of 1000 times, 1ml of ZT element mother liquor with the volume of 1000 times, 2ml of TDZ element mother liquor with the volume of 1000 times, 30g of white sugar, 4.1g of agar after the white sugar is fully dissolved, full stirring, and adding water to fix the volume to 1000 ml. Measuring the temperature by using a thermometer, adjusting the pH value to 5.82 by using an acid-base pH meter, and adding an NHOH solution if the solution is acidic; otherwise, add HCl solution. And subpackaging the prepared solutions into 3.5-inch culture bottles with about 30ml per bottle, placing the culture bottles into a high-temperature sterilizing pot, setting the sterilizing temperature at 121 ℃ and sterilizing for 26 min. And after the sterilization is finished, taking the culture medium out of the high-temperature sterilization pot, and putting the culture medium into a condensing type condenser to finish the preparation of the culture medium.

When carrying out the preparation of culture medium, the required solution measurement is taken out to the accurate volume of needs graduated flask, and when the culture medium was prepared, the solution kind that adopts is more, and the solution measurement of taking simultaneously is little, consequently when taking one of them solution volume back, solution can be attached to in the section of thick bamboo wall of graduated flask, when taking the measurement of next solution again through the graduated flask, can cause the volume to get the error, make this solution of weighing mix with the solution of last weighing, cause the influence to the result of experiment.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a special graduated flask of scirpus marigoldens differentiation proliferation experiment.

The above purpose is realized by the following technical scheme:

a special graduated cylinder for scirpus marigoldianus differentiation and proliferation experiments comprises the following components: a measuring cylinder body and a bottom flat plate seat, wherein a round baffle is arranged below the measuring cylinder body, the round baffle and the measuring cylinder body are of an integral structure, a sliding piston plate is arranged in the measuring cylinder body, a sealing rubber ring is sleeved on the annular concave surface of the sliding piston plate, the circle center position of the bottom of the sliding piston plate is vertically connected with a push rod, the bottom of the push rod is vertically connected with the circle center of the bottom flat plate seat, the circle center of the circular baffle is provided with an insertion hole, two sides of the insertion hole are provided with balance holes with the same diameter, the push rod is inserted into the jack, the balance rod is inserted into the balance hole, the top of the balance rod is vertically connected with the bottom of the sliding piston plate, the bottom of the balancing rod is vertically connected with the bottom flat plate seat, and a metering scale value is processed on the measuring cylinder body.

The special measuring cylinder for the scirpus marigoldianus differentiation and proliferation experiment is characterized in that an upper limiting retaining ring and a lower limiting retaining ring are respectively arranged inside the measuring cylinder body, and the diameters of the upper limiting retaining ring and the lower limiting retaining ring are the same.

The measuring cylinder special for the scirpus marigoldianus differentiation and proliferation experiment is characterized in that two strip-shaped holes or four strip-shaped holes with the same length are formed in the cylinder wall of the measuring cylinder body, and the height of each strip-shaped hole is lower than that of the upper limiting baffle ring.

When the bottom of the sliding piston plate is in contact with the lower limiting retaining ring, the top surface of the sliding piston plate and the 0-scale position of the scale value are on the same horizontal plane, and the measuring cylinder body, the circular baffle and the bottom flat plate seat are made of plastic materials.

The utility model discloses the beneficial effect who reaches is:

1. the utility model has simple structure and convenient operation, when the culture medium for the differentiation and proliferation method experiment of scirpus marigolensis is prepared, the solution to be prepared is firstly poured into the measuring cylinder body, when the required solution is poured into the measuring cylinder body according to the required metering, the solution in the measuring cylinder is poured into the culture bottle, then the bottom flat plate seat is pushed and is connected with the sliding piston plate through a push rod, when the sliding piston plate slides in the measuring cylinder body, the sliding piston plate can push the solution attached to the inner wall of the measuring cylinder body into the strip-shaped hole on the measuring cylinder body, discharging the residual solution from the strip-shaped hole, pulling the sliding piston plate to be attached to the upper surface of the lower limit baffle ring, other solutions are poured into the measuring cylinder body, the cylinder wall of the measuring cylinder body can be guaranteed to be clean, and meanwhile the accuracy of metering when other solutions are poured can be guaranteed.

2. The utility model discloses be provided with the upper limit in the graduated flask body and keep off the ring with lower limit, when the bottom surface of slip piston plate and the top surface contact of lower limit fender ring, the top surface of slip piston plate and 0 scale of scale interval are on same horizontal plane this moment, when the top surface of slip piston plate and the ground contact of upper limit fender ring, can discharge the inside solution of graduated flask body is whole from the bar hole this moment, play spacing effect.

3. The utility model discloses be provided with the balancing pole between slip piston board and bottom flat base, when promoting bottom flat base, can guarantee to install holistic stability, guaranteed the stationarity promoting the slip piston board.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. In the drawings:

fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a top view of fig. 1.

Fig. 3 is a cross-sectional view of fig. 1.

In the figure: 1. the measuring cylinder comprises a measuring cylinder body, 2, a bottom flat plate seat, 3, a circular baffle, 4, a sliding piston plate, 5, a sealing rubber ring, 6, a push rod, 7, a jack, 8, a balance hole, 9, a balance rod, 10, a metering scale value, 11, an upper limiting retaining ring, 12, a lower limiting retaining ring, 13 and a strip-shaped hole.

Detailed Description

The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are presented herein only to illustrate and explain the present invention, and not to limit the present invention.

Example 1:

a special graduated cylinder for scirpus marigoldianus differentiation and proliferation experiments comprises the following components: the measuring cylinder comprises a measuring cylinder body 1 and a bottom flat plate seat 2, wherein a circular baffle 3 is arranged below the measuring cylinder body, the circular baffle and the measuring cylinder body are of an integral structure, a sliding piston plate 4 is arranged inside the measuring cylinder body, a sealing rubber ring 5 is sleeved on an annular concave surface of the sliding piston plate, a push rod 6 is vertically connected with the circle center of the bottom of the sliding piston plate, the bottom of the push rod is vertically connected with the circle center of the bottom flat plate seat, a jack 7 is arranged at the circle center of the circular baffle, balance holes 8 with the same diameter are arranged at two sides of the jack, the push rod is inserted into the jack, a balance rod 9 is inserted into the balance hole, the top of the balance rod is vertically connected with the bottom of the sliding piston plate, and the bottom of the balance rod is vertically connected with the bottom flat plate seat, the measuring cylinder body is processed with a metering scale value 10.

Example 2:

according to the measuring cylinder special for scirpus marigoldianus differentiation and proliferation experiments in embodiment 1, the measuring cylinder body is internally provided with an upper limiting baffle ring 11 and a lower limiting baffle ring 12 respectively, and the diameters of the upper limiting baffle ring and the lower limiting baffle ring are the same.

Example 3:

according to the measuring cylinder special for scirpus marigoldianus differentiation and proliferation experiments in

embodiment

1 or 2, two strip-shaped holes or four strip-shaped holes 13 with the same length are formed in the cylinder wall of the measuring cylinder body, and the height of each strip-shaped hole is lower than that of the upper limiting baffle ring.

Example 4:

according to the measuring cylinder special for scirpus marigoldens differentiation and proliferation experiments in the

embodiment

1, 2 or 3, when the bottom of the sliding piston plate is in contact with the lower limiting baffle ring, the top surface of the sliding piston plate and the 0-scale position of the scale value are on the same horizontal plane, and the measuring cylinder body, the circular baffle and the bottom flat plate seat are made of plastic materials.

Claims

1. A special graduated cylinder for scirpus marigoldianus differentiation and proliferation experiments comprises the following components: graduated flask body and bottom flat seat, characterized by: a circular baffle is arranged below the measuring cylinder body, the circular baffle and the measuring cylinder body are of an integral structure, a sliding piston plate is arranged in the measuring cylinder body, a sealing rubber ring is sleeved on the annular concave surface of the sliding piston plate, the circle center position of the bottom of the sliding piston plate is vertically connected with a push rod, the bottom of the push rod is vertically connected with the circle center of the bottom flat plate seat, the circle center of the circular baffle is provided with an insertion hole, two sides of the insertion hole are provided with balance holes with the same diameter, the push rod is inserted into the jack, the balance rod is inserted into the balance hole, the top of the balance rod is vertically connected with the bottom of the sliding piston plate, the bottom of the balancing rod is vertically connected with the bottom flat plate seat, and a metering scale value is processed on the measuring cylinder body.

2. The special measuring cylinder for scirpus marigolense differentiation and proliferation experiments as claimed in claim 1, wherein: the measuring cylinder is characterized in that an upper limiting retaining ring and a lower limiting retaining ring are respectively arranged in the measuring cylinder body, and the diameters of the upper limiting retaining ring and the lower limiting retaining ring are the same.

3. The special measuring cylinder for scirpus marigolense differentiation and proliferation experiments as claimed in claim 2, wherein: the measuring cylinder is characterized in that two strip-shaped holes with the same length or four strip-shaped holes with the same length are formed in the wall of the measuring cylinder body, and the height of each strip-shaped hole is lower than that of the upper limiting baffle ring.

4. The special measuring cylinder for scirpus marigolense differentiation and proliferation experiments as claimed in claim 2, wherein: when the bottom of the sliding piston plate is in contact with the lower limiting baffle ring, the top surface of the sliding piston plate and the 0-scale position of the scale value are on the same horizontal plane, and the measuring cylinder body, the circular baffle and the bottom flat plate seat are made of plastic materials.