CN212255008U - Streaming application PD background light eliminating device - Google Patents
Streaming application PD background light eliminating device Download PDFInfo
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- CN212255008U CN212255008U CN201922392702.XU CN201922392702U CN212255008U CN 212255008 U CN212255008 U CN 212255008U CN 201922392702 U CN201922392702 U CN 201922392702U CN 212255008 U CN212255008 U CN 212255008U
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Abstract
The utility model discloses a PD background light remove device is used to STREAMING, including laser instrument, voltage input end, PD photodiode, transimpedance amplifier circuit, integral amplifier circuit and voltage output, the PD photodiode is connected to the voltage input end, the PD photodiode is connected to transimpedance amplifier circuit one end, and voltage output is connected to the other end, integral amplifier circuit connects transimpedance amplifier circuit, the laser instrument is just shining PD photodiode. The laser is adopted to directly irradiate the PD photoelectric receiving diode, and after the PD photoelectric receiving diode receives strong laser light source irradiation, the post-stage receiving circuit can generate direct current bias voltage, so that the useful signal receiving range is narrowed. An integrating circuit is used for integrating the direct current bias voltage, and the direct current bias voltage is fed back to an input end and subtracted from the input end to counteract background light of the laser, so that a useful signal can be detected.
Description
Technical Field
The utility model belongs to the technical field of STREAMING electronic circuit, concretely relates to PD background light remove device is used to STREAMING.
Background
A Flow cytometer (Flow cytometer) is a device that automatically analyzes and sorts cells. It can quickly measure, store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can select specified cell subsets according to the preselected parameter range. Most flow cytometers are zero resolution instruments that can only measure an index such as total nucleic acid, total protein, etc. of a cell, but cannot identify and measure the amount of nucleic acid or protein at a particular location. That is, its detail resolution is zero.
Flow cytometry consists essentially of four parts. They are: a flow chamber and a fluid flow system; a laser source and an optical system; a photoelectric tube and a detection system; a computer and an analysis system.
Photoelectric tube and detection system
The fluorescence generated by the fluorescence-stained cells after excitation with suitable light is measured by conversion into an electrical signal by a photoelectric converter. Photomultiplier tubes (PMTs) are most commonly used. The PMT has short response time which is only ns magnitude; the spectral response characteristic is good, and the light quantum yield is high in a spectral region of 200-900 nm. The gain of the photomultiplier tube is continuously adjustable from 10 to 10, and is therefore advantageous for low light measurements. During the operation of the photoelectric tube, the stability problem needs to be particularly noticed, the working voltage needs to be very stable, and the working current and the working power cannot be too large. The general power consumption is lower than 0.5W; the maximum anode current is at a few milliamps. In addition, attention is paid to dark adaptation processing of the photoelectric tube, and good magnetic shielding. In use, the PMTs are installed at different positions, which are not compatible with each other because of different spectral response characteristics. Also useful are silicon photodiodes, which are more stable than PMTs in high light.
The electrical signal output from the PMT is still weak and needs to be amplified before it can be input to the analytical instrument. Two types of amplifiers are typically provided in flow cytometry. One is that the output signal amplitude is linear with the input signal, called linear amplifier. Linear amplifiers are suitable for signals which vary over a relatively small range and also for signals which represent biological linear processes, such as DNA measurements, etc. The other is a logarithmic amplifier, with a common logarithmic relationship between the output signal and the input signal. Logarithmic amplifiers are often used in immunological measurements. Because three subgroups of negative, positive and strong positive are displayed simultaneously in immunoassay, the fluorescence intensity of the subgroups differs by 1-2 orders of magnitude; and in multicolor immunofluorescence measurement, the data collected by a logarithmic amplifier is easy to interpret. In addition, the method has the advantages of convenient adjustment, difficult influence of external working conditions on the distribution shape of cell populations and the like.
In streaming Forward (FSC) applications, the beam of a laser is typically directed at the edge of a PD receiver in order to identify the signal to be detected from strong background light. The useful light signal is irradiated on the PD receiver, which has the disadvantage that the useful signal can only be partially received, and the focusing position of the cell in the flow cell changes to affect the receiving effect.
SUMMERY OF THE UTILITY MODEL
The utility model aims to overcome among the prior art STREAMING electronic circuit and can only accept partial useful signal, the focus position of cell in STREAMING pond changes simultaneously and can influence the not enough of receiving effect, provides a PD background light remove device is used to STREAMING, can guarantee that useful signal is detected out with the background light of offsetting the laser instrument.
For solving the prior art problem, the utility model discloses a PD background light remove device is used to STREAMING, including laser instrument, voltage input end, PD photodiode, transimpedance amplifier circuit, integral amplifier circuit and voltage output end, the PD photodiode is connected to the voltage input end, transimpedance amplifier circuit connects PD photodiode one end, and voltage output end is connected to the other end, integral amplifier circuit connects transimpedance amplifier circuit, the laser instrument is just shining PD photodiode.
Further, the transimpedance amplifier circuit comprises a transimpedance amplifier U1, wherein the inverting input terminal of the transimpedance amplifier U1 is connected with the PD photodiode, the forward input terminal is grounded, and the output terminal is connected with the voltage output terminal.
Further, the transimpedance amplifier circuit further includes a resistor R1, one end of the resistor R1 is connected to the inverting input terminal of the transimpedance amplifier U1, and the other end is connected to the output terminal of the transimpedance amplifier U1.
Further, the integrating and amplifying circuit comprises an integrating amplifier U2, wherein the positive input end of the integrating amplifier U2 is connected with the output end of the transimpedance amplifier U1, the negative input end is grounded, and the output end is connected with the PD photodiode.
Further, the low-pass filter comprises a resistor R2 and a capacitor C1, one end of the resistor R2 is connected with the output end of the transimpedance amplifier U1, the other end of the resistor R2 is connected with the positive input end of the integrating amplifier U2, one end of the capacitor C1 is connected with the resistor R2, and the other end of the capacitor C1 is grounded.
Furthermore, the integrating and amplifying circuit further comprises a resistor R3, a resistor R4 and a capacitor C2, one end of the resistor R3 is connected with the reverse input end of the integrating amplifier U2, the other end of the resistor R3 is grounded, one end of the capacitor C2 is connected with the reverse input end of the integrating amplifier U2, the other end of the capacitor C2 is connected with the output end of the integrating amplifier U2, one end of the resistor R4 is connected with the PD photodiode, and the other end of the resistor R4 is connected with the output end of the integrating.
The utility model discloses beneficial effect who has:
the laser is adopted to directly irradiate the PD photoelectric receiving diode, and after the PD photoelectric receiving diode receives strong laser light source irradiation, the post-stage receiving circuit can generate direct current bias voltage, so that the useful signal receiving range is narrowed. An integrating circuit is used for integrating the direct current bias voltage, and the direct current bias voltage is fed back to an input end and subtracted from the input end to counteract background light of the laser, so that a useful signal can be detected.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Reference numerals: the photoelectric detector comprises a PD photodiode, a trans-impedance amplifier U1, an integrating amplifier U2, a resistor R1, a resistor R2, a resistor R3, a resistor R4, a capacitor C1 and a capacitor C2.
Detailed Description
The present invention will be further described with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
As shown in fig. 1, the utility model discloses a PD background light remove device is used to STREAMING, a serial communication port, including voltage input end, PD photodiode, transimpedance amplifier circuit, integral amplifier circuit and voltage output end, voltage input end connects PD photodiode's positive pole, transimpedance amplifier circuit one end connects PD photodiode's negative pole, and voltage output end is connected to the other end, integral amplifier circuit connects transimpedance amplifier circuit. The transimpedance amplifier circuit and the integral amplifier circuit are rear-stage receiving circuits, a laser is adopted to directly irradiate the PD photoelectric receiving diode, and after the PD photoelectric receiving diode receives strong laser light source irradiation, the rear-stage receiving circuit can generate direct current bias voltage, so that the useful signal receiving range is reduced.
The transimpedance amplification circuit comprises a transimpedance amplifier U1, the reverse input end of the transimpedance amplifier U1 is connected with the cathode of the PD photodiode, the forward input end is grounded, and the output end is connected with the voltage output end. The transimpedance amplifier circuit further comprises a resistor R1, wherein one end of the resistor R1 is connected with the reverse input end of the transimpedance amplifier U1, and the other end of the resistor R1 is connected with the output end of the transimpedance amplifier U1. The integrating and amplifying circuit comprises an integrating amplifier U2, wherein the forward input end of the integrating amplifier U2 is connected with the output end of the transimpedance amplifier U1, the reverse input end of the integrating amplifier U2 is grounded, and the output end of the integrating amplifier U2 is connected with the cathode of the PD photodiode. The low-pass filter comprises a resistor R2 and a capacitor C1, one end of the resistor R2 is connected with the output end of the transimpedance amplifier U1, the other end of the resistor R2 is connected with the positive input end of the integrating amplifier U2, one end of the capacitor C1 is connected with the resistor R2, and the other end of the capacitor C1 is grounded. The integrating and amplifying circuit further comprises a resistor R3, a resistor R4 and a capacitor C2, one end of the resistor R3 is connected with the reverse input end of the integrating amplifier U2, the other end of the resistor R3 is grounded, one end of the capacitor C2 is connected with the reverse input end of the integrating amplifier U2, the other end of the capacitor C2 is connected with the output end of the integrating amplifier U2, one end of the resistor R4 is connected with the cathode of the PD photodiode, and the other end of the resistor R4 is connected with the output end.
In a Forward (FSC) application of a streaming circuit, a PD photodiode is used as a sensor for converting an optical signal into an electrical signal, and is used for detecting the forward optical signal, and strong light of a laser is irradiated onto the PD photodiode, so that the PD photodiode generates a large photocurrent, and the photocurrent is saturated after passing through a transimpedance amplifier U1, and thus the forward signal cannot be detected. The resistor R2 and the capacitor C1 sample the output of the transimpedance amplifier U1, low-frequency and direct-current signals directly pass through a low-pass filter formed by the resistor R2 and the capacitor C1 and enter an integrating and amplifying circuit, and the integrating and amplifying circuit integrates the low-frequency and direct-current signals. The long-time integral signal level of the alternating current signal is 0, only the direct current signal is amplified and fed back to the inverting input end of the transimpedance amplifier U1, and therefore the direct current signal is removed, and the influence of background laser is eliminated.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be considered as the protection scope of the present invention.
Claims (6)
1. A streaming application PD background light eliminating device is characterized by comprising a laser, a voltage input end, a PD photodiode, a transimpedance amplification circuit, an integral amplification circuit and a voltage output end, wherein the voltage input end is connected with the PD photodiode, one end of the transimpedance amplification circuit is connected with the PD photodiode, the other end of the transimpedance amplification circuit is connected with the voltage output end, the integral amplification circuit is connected with the transimpedance amplification circuit, and the laser just irradiates the PD photodiode.
2. A streaming PD backlight elimination device according to claim 1, characterized in that said transimpedance amplifier circuit comprises a transimpedance amplifier (U1), the inverting input of said transimpedance amplifier (U1) being connected to the PD photodiode, the forward input being connected to ground, and the output being connected to the voltage output.
3. A streaming PD backlight elimination device according to claim 2, characterized in that said transimpedance amplifier circuit further comprises a resistor (R1), said resistor (R1) being connected at one end to the inverting input of the transimpedance amplifier (U1) and at the other end to the output of the transimpedance amplifier (U1).
4. A streaming PD backlight elimination device according to claim 1, characterized by that, said integrating and amplifying circuit comprises an integrating amplifier (U2), the forward input terminal of said integrating amplifier (U2) is connected to the output terminal of the transimpedance amplifier (U1), the reverse input terminal is connected to ground, and the output terminal is connected to the PD photodiode.
5. A streaming PD background light elimination apparatus according to claim 4, further comprising a low-pass filter, said low-pass filter comprising a resistor (R2) and a capacitor (C1), said resistor (R2) having one end connected to the output terminal of the transimpedance amplifier (U1) and the other end connected to the positive input terminal of the integrating amplifier (U2), said capacitor (C1) having one end connected to the resistor (R2) and the other end connected to ground.
6. A streaming PD background light elimination device according to claim 5, characterized in that the integrating and amplifying circuit further comprises a resistor (R3), a resistor (R4) and a capacitor (C2), wherein one end of the resistor (R3) is connected with the inverting input terminal of the integrating amplifier (U2), the other end is grounded, one end of the capacitor (C2) is connected with the inverting input terminal of the integrating amplifier (U2), the other end is connected with the output terminal of the integrating amplifier (U2), one end of the resistor (R4) is connected with the PD photodiode, and the other end is connected with the output terminal of the integrating amplifier (U2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922392702.XU CN212255008U (en) | 2019-12-27 | 2019-12-27 | Streaming application PD background light eliminating device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922392702.XU CN212255008U (en) | 2019-12-27 | 2019-12-27 | Streaming application PD background light eliminating device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212255008U true CN212255008U (en) | 2020-12-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922392702.XU Active CN212255008U (en) | 2019-12-27 | 2019-12-27 | Streaming application PD background light eliminating device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212255008U (en) |
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2019
- 2019-12-27 CN CN201922392702.XU patent/CN212255008U/en active Active
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Effective date of registration: 20231218 Address after: 230000, West Half Floor, 2nd Floor, Building 2, Phase II, Intelligent Science and Technology Park, No. 3959 Susong Road, Hefei Economic and Technological Development Zone, Anhui Province Patentee after: Zhongsheng Medical Technology (Hefei) Co.,Ltd. Address before: 215163 Room 101, building 3, No.8 Jinfeng Road, high tech Zone, Suzhou City, Jiangsu Province Patentee before: ZHONGSHENG (SUZHOU) MEDICAL TECHNOLOGY CO.,LTD. |
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