CN212247082U - 3D cell culture dish - Google Patents

3D cell culture dish Download PDF

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Publication number
CN212247082U
CN212247082U CN202020835121.9U CN202020835121U CN212247082U CN 212247082 U CN212247082 U CN 212247082U CN 202020835121 U CN202020835121 U CN 202020835121U CN 212247082 U CN212247082 U CN 212247082U
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China
Prior art keywords
culture dish
cell culture
dish
arc
fixed bolster
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CN202020835121.9U
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Chinese (zh)
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刘逸宁
林建宁
梁晓东
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Shenzhen Andikang Biotechnology Co ltd
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Shenzhen Andikang Biotechnology Co ltd
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Abstract

The utility model relates to a cell culture technical field, concretely relates to 3D cell culture dish. The specific technical scheme is as follows: the utility model provides a 3D cell culture dish, includes the culture dish that has the dish lid and is used for placing the base plate of a plurality of culture dish, be provided with the fixed bolster in the culture dish, the top edge of fixed bolster is provided with the crotch that the several caught on the culture dish edge and buckle outward to the culture dish, the symmetry is provided with two arc walls on the culture dish inner wall, be provided with on the lateral wall of fixed bolster with the arch of arc wall looks adaptation. The utility model provides an among the prior art can't carry out the problem of cell culture to different cell types.

Description

3D cell culture dish
Technical Field
The utility model relates to a cell culture technical field, concretely relates to 3D cell culture dish.
Background
The cell culture is to take out the tissue or organ from animal body by aseptic technique, simulate the physiological condition in animal body, culture in vitro, and make it continuously grow and reproduce, so that people can observe the life phenomena of the processes of cell growth, reproduction, cell differentiation, cell aging, etc.
In the prior art, when a fixed support is used for culturing cells, the fixed support is generally placed at the bottom of a culture dish. However, the placement of the fixation scaffold varies for different cell types. For example, in the case of culturing a cell type growing at the gas-liquid interface, the culture medium is immersed on the lower surface of the fixed support; when the cell types with low metabolic activity and proliferation rate are cultured conventionally, the culture medium is immersed on the upper surface and the lower surface of the fixed support; when the cell types with higher metabolic activity and proliferation rate are cultured conventionally, the whole fixed support is immersed in the culture medium, and the culture mediums on the upper surface and the lower surface are communicated. Therefore, it is necessary to provide a cell culture dish capable of flexibly adjusting the height of a fixing frame when culturing different types of cells.
Disclosure of Invention
Not enough to prior art, the utility model provides a 3D cell culture dish has solved among the prior art and can't carry out cell culture's problem to different cell types.
In order to achieve the above purpose, the utility model discloses a following technical scheme realizes:
the utility model discloses a 3D cell culture dish, including the culture dish that has the ware lid and the base plate that is used for placing a plurality of culture dish, be provided with the fixed bolster in the culture dish, the top edge of fixed bolster is provided with the crotch that the several caught on the culture dish edge and buckle outward to the culture dish, the symmetry is provided with two arcs on the culture dish inner wall, be provided with on the lateral wall of fixed bolster with the arch of arc groove looks adaptation.
Preferably, the base plate is provided with a plurality of grooves for placing the culture dishes, the surface of the base plate is transversely provided with a plurality of supporting tables extending towards the center of the groove, the bottom of the groove is transversely provided with a supporting plate, and the supporting plate and the groove are fixed through a telescopic rod.
Preferably, fixing columns which are mutually crossed are fixed at the bottom of the culture dish, fixing grooves are formed in two ends of each fixing column along the length direction of the fixing column, a Z-shaped pressing rod is arranged in two fixing grooves of one fixing column, and the other end of each pressing rod extends out of the groove; when the culture dish is placed on the supporting plate, the bottom surface of the hook is just abutted to the table top of the supporting table.
Preferably, the height of the bottom edge of the dish cover is lower than the length of the hook on the outer wall of the culture dish.
Preferably, the inner top surface of the dish cover is provided with a notch, so that the hook positioned on the edge of the top of the culture dish is arranged in the notch.
Preferably, each row of arc-shaped grooves are sequentially provided with a plurality of arc-shaped grooves from top to bottom.
Preferably, when the bottom of the fixing support is in contact with the bottom of the culture dish, the protrusion is placed in the arc-shaped groove close to the bottom of the culture dish.
Preferably, the end face of the support platform is arc-shaped and is attached to the outer wall of the culture dish.
The utility model discloses possess following beneficial effect:
the utility model discloses be provided with the adjustable fixed bolster of height from top to bottom in the culture dish, specifically through setting up symmetrical two arc walls on the inner wall of culture dish, the symmetry is provided with two archs on the lateral wall of fixed bolster, when the bottom of culture dish is arranged in to the fixed bolster, the arch is arranged in the arc wall near the culture dish bottom, the culture dish is removed to the accessible, arrange the arch in the arc wall of co-altitude not, thereby adjust the height of fixed bolster in the culture dish, at this moment, when arranging the culture dish in the recess of base plate, the culture dish has certain distance apart from the bottom of recess.
If the height of the fixing support is adjusted in the process of culturing cells, fixing columns with fixing grooves at two ends are arranged at the bottom of the culture dish in a crossed mode, and pressure rods are inserted into the fixing grooves. Simultaneously, connect the backup pad through a plurality of telescopic links in the bottom of recess, arrange the culture dish in the backup pad on, make the culture dish downstream through the depression bar that pushes down, and the fixed bolster keeps motionless. Thus, different heights of the fixed support within the culture dish are achieved by moving the culture dish. Moreover, in the culture process, the height of the fixed support in the culture dish can be adjusted at any time according to needs, the flexibility is good, and when the fixed support is adjusted, the dish cover does not need to be taken, so that external pollutants are prevented from entering the culture dish, and the growth environment of cells is ensured.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of a capsule structure;
FIG. 3 is a schematic view of a cross arrangement structure of two fixing posts;
FIG. 4 is a schematic view of the structure of the pressure lever;
FIG. 5 is a schematic structural view showing the present invention disposed in a groove when only one groove is formed on the substrate;
FIG. 6 is a top view of FIG. 5;
FIG. 7 is a schematic view of a substrate having a plurality of grooves;
in the figure: dish lid 1, culture dish 2, base plate 3, fixed bolster 4, crotch 5, recess 6, brace table 7, backup pad 8, telescopic link 9, fixed column 10, fixed slot 11, depression bar 12, breach 13.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Unless otherwise indicated, the technical means used in the examples are conventional means well known to those skilled in the art.
Referring to fig. 1-4, the utility model discloses a 3D cell culture dish, including culture dish 2 that has ware lid 1 and the base plate 3 that is used for placing a plurality of culture dish 2, be provided with fixed bolster 4 in the culture dish 2, the top edge of fixed bolster 4 is provided with the crotch 5 that the several caught on culture dish 2 edge and outside the bend of culture dish 2, the symmetry is provided with two arcs on the 2 inner walls of culture dish, be provided with the arch with arc groove looks adaptation on the lateral wall of fixed bolster 4, the arc setting leans on the position at culture dish 2 middle parts, avoids the culture medium to arrange in the arc. It should be understood that: a plurality of arc-shaped grooves are sequentially arranged on each row of arc-shaped grooves from top to bottom at equal intervals, and the arc-shaped grooves on each row are symmetrical. When the bottom of the fixed support 4 is contacted with the bottom of the culture dish 2, namely, the fixed support 4 is completely arranged at the bottom of the culture dish 2; the bulges are arranged in the arc-shaped grooves close to the bottom of the culture dish 2, namely, only two bulges are arranged on the outer side wall of the fixed support 4 and are symmetrically arranged. The bulge is made of plastic, the diameter of the bulge is smaller than that of the arc-shaped groove, and when the fixing support 4 is lifted upwards, the bulge can be moved out of the arc-shaped groove and enters the next arc-shaped groove. Moreover, a certain gap is formed between the outer side wall of the fixing bracket 4 and the inner wall of the culture dish 2, so that the fixing bracket 4 can move up and down conveniently.
Referring to fig. 5-6, when only one groove 6 for placing the culture dish 2 is formed in the substrate 3, a plurality of supporting bases 7 extending toward the center of the groove 6 are transversely formed on the surface of the substrate 3, and the end surfaces of the supporting bases 7 are arc-shaped and attached to the outer wall of the culture dish 2. The bottom of recess 6 transversely is provided with backup pad 8, and is fixed through telescopic link 9 between backup pad 8 and the recess 6. It should be understood that: the telescopic link 9 can set up a plurality ofly, and the setting of backup pad 8 and telescopic link 9 is convenient for adjust the height of culture dish 2 in recess 6 to the height of adjustment fixed bolster 4 in culture dish 2, thereby the cell culture of adaptation different grade type.
Further, in order to adjust the height of the fixing bracket 4 in the culture dish 2 during the process of culturing the cells, fixing columns 10 are fixed at the bottom of the culture dish 2 and are arranged to intersect with each other, the two fixing columns 10 have a certain angle and are arranged according to actual conditions, and in order to avoid the fixing columns 10 from shaking on the support plate 8, the fixing columns 10 are arranged to be rectangular, the length of the fixing columns is greater than half of the diameter of the culture dish 2, and therefore the stability of the culture dish 2 is improved. Fixing grooves 11 are formed in two ends of each fixing column 10 along the length direction of the fixing column, and the sum of the lengths of the two fixing grooves 11 is smaller than that of the fixing column 10. Two fixing grooves 11 of one fixing column 10 are internally provided with a Z-shaped pressing rod 12, the other end of the pressing rod 12 extends out of the groove 6, and the length of a cross rod below the pressing rod 12 is larger than that of a cross rod above the pressing rod; when the culture dish 2 is placed on the support plate 8, the bottom surface of the hook 5 is just abutted against the table surface of the support table 7. It should be understood that: the height of the bottom edge of the dish cover 1 is lower than the length of the hook 5 on the outer wall of the culture dish 2, namely the hook 5 extends out of the dish cover 1, so that the height of the fixed support 4 in the culture dish 2 is adjusted without moving the dish cover 1. The pressing rod 12 penetrates out of the space between the two adjacent supporting platforms 7, and the cross rod above the pressing rod 12 is far away from the plate surface of the substrate 3. Moreover, when the projection is positioned in the arc-shaped groove close to the opening of the culture dish 2, the cross rod above the pressure rod 12 is just arranged on the plate surface of the base plate 3.
Further, in order to avoid the dish lid 1 to move on the culture dish 2 or drop because of the touching, be provided with breach 13 at the interior top surface of dish lid 1, make the crotch 5 that is located on 2 top edges of culture dish just arrange in breach 13, and culture dish 2 is except contacting with top surface and the interior top surface contact of dish lid 1 with crotch 5 except, thereby place dish lid 1 stably on culture dish 2, even adjust the height of fixed bolster 4, dish lid 1 also can be stabilized on crotch 5 of card on fixed bolster 4.
Referring to FIG. 7, a plurality of grooves 6 for holding culture dishes 2 are formed on a substrate 3, so that cells of different types or the same type can be cultured simultaneously in a large scale.
When using the utility model discloses the time, adjust the bottom of culture dish 2 with fixed bolster 4 in advance, put culture medium and cell lid good dish lid 1. Then, a zigzag pressing rod 12 is inserted into two fixing grooves 11 of one of the fixing posts 10, or a pressing rod 12 is inserted into each fixing groove 11, and the arrangement is performed according to actual conditions. Then, the press lever 12 is lifted to move the culture dish 2 into the groove 6 on the substrate 3, and the culture dish 2 is placed in the support plate 8, and at this time, the bottom surface of the hook 5 located outside the culture dish 2 is placed on the outside of the support table 7, thereby performing 3D culture of the cells.
If at the in-process of carrying out the cultivation to the cell, need adjust the height of fixed bolster 4, only need push down depression bar 12, telescopic link 9 is compressed, backup pad 8 moves down, depression bar 12 takes culture dish 2 downstream, because the crotch 5 of fixed bolster 4 offsets with a supporting bench 7, therefore, only culture dish 2 downstream, the arch moves towards the arc wall of 2 openings parts of culture dish gradually, adjust suitable position until fixed bolster 4, simultaneously, the arc wall is arranged in to the arch, thereby fix fixed bolster 4 in culture dish 2, until accomplishing the cultivation of cell.
In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are merely for convenience of description of the present invention, and do not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
The above-mentioned embodiments are only intended to describe the preferred embodiments of the present invention, but not to limit the scope of the present invention, and those skilled in the art should also be able to make various modifications and improvements to the technical solution of the present invention without departing from the spirit of the present invention, and all such modifications and improvements are intended to fall within the scope of the present invention as defined in the appended claims.

Claims (8)

1. The utility model provides a 3D cell culture dish, includes culture dish (2) that have dish lid (1) and base plate (3) that are used for placing a plurality of culture dish (2), its characterized in that: be provided with fixed bolster (4) in culture dish (2), the top edge of fixed bolster (4) is provided with crotch (5) that the several caught on culture dish (2) edge and buckled outward to culture dish (2), the symmetry is provided with two arcs on culture dish (2) inner wall, be provided with on the lateral wall of fixed bolster (4) with the arch of arc groove looks adaptation.
2. A 3D cell culture dish according to claim 1, wherein: be provided with a plurality of on base plate (3) and place recess (6) of culture dish (2), transversely be provided with the orientation on base plate (3) face recess (6) center extension's several brace table (7), the bottom of recess (6) transversely is provided with backup pad (8), it is fixed through telescopic link (9) between backup pad (8) and recess (6).
3. A 3D cell culture dish according to claim 2, wherein: fixing columns (10) which are mutually crossed are fixed at the bottom of the culture dish (2), fixing grooves (11) are formed in two ends of each fixing column (10) along the length direction of the fixing column, Z-shaped pressing rods (12) are arranged in the two fixing grooves (11) of one fixing column (10), and the other ends of the pressing rods (12) extend out of the grooves (6); when the culture dish (2) is placed on the supporting plate (8), the bottom surface of the hook (5) is just propped against the table top of the supporting table (7).
4. A 3D cell culture dish according to claim 1, wherein: the height of the bottom edge of the dish cover (1) is lower than the length of the hook (5) on the outer wall of the culture dish (2).
5. A 3D cell culture dish according to claim 1, wherein: and a notch (13) is formed in the inner top surface of the dish cover (1), so that the hook (5) positioned on the top edge of the culture dish (2) is arranged in the notch (13).
6. A 3D cell culture dish according to claim 1, wherein: each row of arc-shaped grooves are sequentially provided with a plurality of arc-shaped grooves from top to bottom.
7. A3D cell culture dish according to claim 6, wherein: when the bottom of the fixed support (4) is contacted with the bottom of the culture dish (2), the bulge is arranged in the arc-shaped groove close to the bottom of the culture dish (2).
8. A 3D cell culture dish according to claim 2, wherein: the end face of the supporting platform (7) is arc-shaped and is attached to the outer wall of the culture dish (2).
CN202020835121.9U 2020-05-19 2020-05-19 3D cell culture dish Active CN212247082U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202020835121.9U CN212247082U (en) 2020-05-19 2020-05-19 3D cell culture dish

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202020835121.9U CN212247082U (en) 2020-05-19 2020-05-19 3D cell culture dish

Publications (1)

Publication Number Publication Date
CN212247082U true CN212247082U (en) 2020-12-29

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ID=74001255

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202020835121.9U Active CN212247082U (en) 2020-05-19 2020-05-19 3D cell culture dish

Country Status (1)

Country Link
CN (1) CN212247082U (en)

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