CN212232863U

CN212232863U - Immune cell preservation device

Info

Publication number: CN212232863U
Application number: CN202020721688.3U
Authority: CN
Inventors: 林俊堂
Original assignee: Henan Intercell Biology Technology Co ltd
Current assignee: Henan Intercell Biology Technology Co ltd
Priority date: 2020-05-06
Filing date: 2020-05-06
Publication date: 2020-12-29
Anticipated expiration: 2030-05-06

Abstract

The utility model relates to an immune cell preservation device, including box and the case lid that is used for sealed box of setting at the box top, one side of case lid is articulated mutually with the box, fixed connection can be dismantled with the box to the opposite side of case lid, the inside platform of placing that is used for placing the cryopreserved pipe that is equipped with of box, fixed connection can be dismantled on the inner wall of bottom half to the bottom of placing the platform, place the bench and set up the ascending standing groove that is used for placing the cryopreserved pipe of a plurality of openings, the vertical setting of standing groove is placing the platform inside and extending to the lower part of placing the platform, fixedly connected with prevents shaking the movable coil on the inner wall on standing groove upper portion, the cross incision has been seted up to the centre of preventing shaking the movable coil, the inside of placing the platform is equipped with a plurality of. The device can fix the cryopreserved pipe, and has fine cushioning effect, can prevent that the cryopreserved pipe from rocking, avoids the cryopreserved pipe to make a round trip to rock in the box when bumping with box inner wall or cold source and breakage.

Description

Immune cell preservation device

Technical Field

The utility model belongs to the technical field of the cell is stored, especially relate to an immune cell save set.

Background

Immune cells refer to cells involved in or associated with an immune response, including lymphocytes, dendritic cells, monocytes/macrophages, granulocytes, mast cells, and the like. The research on immune cells has become a hot spot in the technical field of biological treatment, and the immune cells play an important role in the research on disease pathology and the development of biological cell therapy. The immune cells are not stored at low temperature regardless of research or treatment, and after the immune cells are collected, the immune cells need to be placed into a freezing tube, and are placed in a refrigerator at 80 ℃ below zero after being cooled to 80 ℃ below zero in a programmed manner for storage, or the samples are placed in the refrigerator at 80 ℃ below zero for storage for 24 hours and then transferred to a liquid nitrogen tank at 196 ℃ below zero for storage. When immune cells need to be transported, in order to ensure that the immune cells still keep a frozen state in the transportation process, a low-temperature preservation device needs to be adopted for transportation. However, the existing immune cell preservation device is mostly a box body with a simple structure, the structural design is unreasonable, the freezing tube cannot be well fixed in the preservation device, the freezing tube swings back and forth in the box body when the box body is bumped, and the freezing tube is easily broken due to collision with the inner wall of the box body or a cold source.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing an immune cell preservation device to it is unreasonable to solve current immune cell preservation device structural design, freezes in the transportation and deposits the pipe and make a round trip to rock in the preservation device, leads to freezing to deposit the pipe easily and takes place broken technical problem.

In order to solve the technical problem, the utility model discloses the technical scheme who adopts is:

the utility model provides an immune cell preservation device, includes the box and sets up the case lid that is used for sealed box at the box top, one side of case lid is articulated mutually with the box, and fixed connection can be dismantled with the box to the opposite side of case lid, and the inside platform of placing that is used for placing the cryopreserved pipe that is equipped with of box, fixed connection can be dismantled on the bottom of placing the platform on the inner wall of bottom half, places the bench and offers the ascending standing groove that is used for placing the cryopreserved pipe of a plurality of openings, the standing groove is vertical to be set up and extends to the lower part of placing the platform inside placing the platform, fixedly connected with prevents shaking the movable coil on the inner wall on standing groove upper portion, the cross incision has been seted up to the centre of preventing shaking the movable coil, the inside of placing the platform is equipped with a plurality of.

Clamping mechanism sets up the splint that are used for pressing from both sides tight cryopreserving pipe inside the standing groove including the symmetry, place the inside guide way that is the font that sets up of symmetry, sliding connection has the guide bar in the guide way, the one end of guide bar is located the guide way, the other end of guide bar extend to the standing groove in and with splint fixed connection, the guide bar periphery cover is equipped with compression spring, compression spring's one end and splint fixed connection, compression spring's the other end and the inner wall fixed connection of standing groove, the stopper that is used for preventing the guide bar from breaking away from inside the guide way that is located two symmetries of fixedly connected with setting on the guide bar of inside of guide way.

Four angle departments fixedly connected with longitudinal section of placing the platform bottom are the fixture block of T shape, fixedly connected with on the inner wall of bottom half with the mount pad of fixture block one-to-one, set up in the mount pad with fixture block matched with draw-in groove, place the platform and pass through the fixture block can dismantle fixed connection on the inner wall of bottom half.

The longitudinal section of mount pad is trapezoidal, equal first damping spring of fixedly connected with on two sides about the fixture block, first damping spring deviates from the one end of fixture block and the inner wall fixed connection of mount pad, has all laid the rubber shock pad on the bottom surface of mount pad inside and the bottom of standing groove.

And a second damping spring is fixedly connected between the periphery of the outside of the placing table and the inner side wall of the box body.

The standing table is cylindrical, and the standing groove is the circular recess, and the standing groove divide into three groups, includes the aperture bank of cells, mesopore bank of cells, the macropore bank of cells that set gradually from the intermediate position of standing table to its edge, and the aperture bank of cells includes a plurality of aperture grooves, and mesopore bank of cells includes a plurality of mesopore grooves, and macropore bank of cells includes a plurality of macropore grooves, and the internal diameter of mesopore groove is greater than the internal diameter of aperture groove and is less than the internal diameter of macropore groove, and the degree of depth of mesopore groove is greater than the degree of depth of aperture groove and is less than the degree of depth of macropore groove, and the even interval setting in circumference of standing table top surface is all followed.

The box body is of a composite layer structure and comprises a heat preservation layer, a buffer layer and a waterproof layer which are sequentially arranged from inside to outside.

The anti-shaking ring is made of an elastic material.

The top of the box cover is fixedly connected with a lifting handle.

The limiting blocks and the clamping blocks are made of elastic rubber.

The utility model has the advantages that: (1) the freezing and storing pipe is fixed by arranging the anti-shaking ring and the clamping mechanism, the freezing and storing pipe is inserted into the placing groove from the cross-shaped incision on the anti-shaking ring, because the anti-shaking ring has elasticity, the anti-shaking ring becomes a four-petal structure which is inclined downwards under the extrusion of the freezing storage pipe, the four-petal structure is enclosed into a cone, the freezing tube is wrapped in the cone structure, the cone structure extrudes the outer wall of the freezing tube under the action of self resilience force, thereby playing a role of fixing, the freezing tube extrudes the clamping plate to enable the guide rod to move towards the direction back from the clamping plate, thereby extruding the compression spring which can apply resilience force to the clamping plate to enable the clamping plate to clamp the freezing tube, the compression spring can buffer the clamping plate, so that the freezing pipe can be prevented from shaking, a certain buffering effect is achieved, and the freezing pipe is prevented from shaking to cause damage; (2) the device is subjected to damping treatment by arranging the first damping spring, the second damping spring and the rubber damping pad, so that the vibration of the freezing storage pipe can be reduced, and the shaking of the freezing storage pipe is further reduced; (3) the placing grooves are three groups with different inner diameters and depths, so that the freezing pipes with different specifications can be fixed at the same time, the application range is improved, and the cost is saved; (4) the box is the composite bed structure for the box has waterproof, shockproof and heat retaining function of buffering concurrently, improves the save effect.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a rear view of FIG. 1;

FIG. 3 is a cross-sectional view of FIG. 1;

FIG. 4 is an enlarged view taken at A in FIG. 3;

FIG. 5 is an enlarged view at B in FIG. 3;

fig. 6 is a schematic structural view of the placement stage.

Reference numerals: the box comprises a box body 1, a box cover 2, a box cover 3, a heat insulation layer 3, a buffer layer 4, a waterproof layer 5, a handle 6, a hinge 7, a lock catch 8, a placing table 9, a clamping block 10, a mounting seat 11, a clamping groove 12, a first damping spring 13, a rubber damping pad 14, a second damping spring 15, a placing groove 16, a shaking prevention moving ring 18, a cross notch 19, a clamping mechanism 20, a small hole groove 20, a middle hole groove 21, a big hole groove 22, a clamping plate 23, a guide rod 24, a compression spring 25, a guide groove 26, a limiting block 27 and a freezing storage pipe 28.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, embodiments of the present invention are described in further detail below with reference to the accompanying drawings.

As shown in fig. 1 and 2, the utility model discloses a box 1 and the case lid 2 that is used for sealed box 1 of setting at 1 top of box, box 1 are composite layer structure, include heat preservation 3, buffer layer 4, the waterproof layer 5 that sets gradually from inside to outside, and the top fixedly connected with handle 6 of case lid 2 makes things convenient for portable, and one side of case lid 2 is articulated mutually through hinge 7 with box 1, and the opposite side of case lid 2 passes through hasp 8 with box 1 and is connected. As shown in fig. 3 and 6, a placing table 9 for placing the freezing tube 28 is provided inside the box body 1, and the placing table 9 is cylindrical. As shown in fig. 5, four corners of the bottom of the placing table 9 are fixedly connected with a fixture block 10 having a T-shaped longitudinal section, the fixture block 10 is made of elastic rubber, the inner wall of the bottom of the box 1 is fixedly connected with mounting seats 11 corresponding to the fixture blocks 10 one by one, the longitudinal section of the mounting seat 11 is trapezoidal, the mounting seat 11 is internally provided with a clamping groove 12 matching with the fixture block 10, the fixture block 10 has elasticity, when in installation, the end of the fixture block 10 is deformed by pressing the fixture block 10 to smoothly enter the clamping groove 12, the placing table 9 is detachably and fixedly connected to the inner wall of the bottom of the box 1 through the fixture block 10, the left and right side surfaces of the fixture block 10 are both fixedly connected with first damping springs 13, one end of the first damping spring 13 departing from the fixture block 10 is fixedly connected with the inner wall of the mounting seat 11, the first damping spring 13 has a good buffering effect on the, further, the shaking of the freezing tube 28 placed in the placing table 9 is reduced, the preservation effect is improved, and the rubber shock-absorbing pad 14 is laid on the bottom surface inside the mounting seat 11, so that the shock-absorbing effect is further improved. As shown in fig. 2, a second damping spring 15 is fixedly connected between the periphery of the outside of the placing table 9 and the inner side wall of the box body 1, a plurality of placing grooves 16 with upward openings are formed in the placing table 9 and used for placing the cryopreservation tubes 28, the placing grooves 16 are vertically arranged in the placing table 9 and extend towards the lower portion of the placing table 9, rubber damping cushions 14 are laid at the bottom of the placing grooves 16, the inner diameter of each placing groove 16 is slightly larger than the outer diameter of each cryopreservation tube 28, an anti-shaking moving ring 17 is fixedly connected to the inner wall of the upper portion of each placing groove 16, a cross-shaped notch 18 is formed in the middle of each anti-shaking moving ring 17, each anti-shaking moving ring 17 is made of an elastic material, and if elastic rubber, elastic plastic and the like can be selected, a plurality of clamping mechanisms 19 for clamping the outer side walls of the cryopreservation. As shown in fig. 6, the placement grooves 16 are divided into three groups, including a small hole groove group, a middle hole groove group, and a large hole groove group, which are sequentially arranged from the middle position of the placement table 9 to the edge thereof, the small hole groove group includes a plurality of small hole grooves 20, the middle hole groove group includes a plurality of middle hole grooves 21, the large hole groove group includes a plurality of large hole grooves 22, the inner diameter of the middle hole groove 21 is greater than the inner diameter of the small hole grooves 20 and is less than the inner diameter of the large hole grooves 22, the depth of the middle hole grooves 21 is greater than the depth of the small hole grooves 20 and is less than the depth of the large hole grooves 22, and each group of placement grooves 16 is uniformly arranged along the circumferential direction of the. As shown in fig. 4, the clamping mechanism 19 includes a clamp plate 23 symmetrically disposed inside the placement groove 16 for clamping the cryopreservation tube 28, a convex-shaped guide groove 26 symmetrically disposed inside the placement table 9 is formed, that is, the diameter of the opening of the guide groove 26 is smaller than the diameter of the inside of the guide groove, a guide rod 24 is slidably connected in the guide groove 26, one end of the guide rod 24 is located in the guide groove 26, the other end of the guide rod 24 extends into the placement groove 16 and is fixedly connected with the clamp plate 23, a compression spring 25 is sleeved on the outer periphery of the guide rod 24, one end of the compression spring 25 is fixedly connected with the clamp plate 23, the other end of the compression spring 25 is fixedly connected with the inner wall of the placement groove 16, two symmetrically disposed stop blocks 27 for preventing the guide rod 24 from being disengaged from the inside of the guide groove 26 are fixedly connected to the guide rod 24 located inside the guide groove 26, the stop blocks, when the guide rod 24 is fitted into the guide groove 26, the stopper 27 is deformed by being pressed, so that the guide rod 24 smoothly enters the guide groove 26.

Before freezing and depositing pipe 28 and not inserting standing groove 16, distance between two splint 23 is less than the external diameter that freezes and deposits pipe 28, when freezing and depositing pipe 28 inserts standing groove 16, it extrudees to splint 23 and makes guide bar 24 move to the direction of keeping away from splint 23 to freezing and depositing pipe 28, thereby extrude compression spring 25, compression spring 25 can exert the resilience force to splint 23, make splint 23 press from both sides tight freezing and depositing pipe 28, set up the side that splint 23 and freezing and depositing pipe 28 contacted into the rough surface, take place to slide when preventing splint 23 extrusion freezing and depositing pipe 28.

When the utility model is used, dry ice or liquid nitrogen is added into a gap between the box body 1 and the placing platform 9 in advance, when the temperature in the box body 1 is reduced to about 80 ℃ below zero, a proper placing groove 16 is selected according to the pipe diameter and the length of the freezing tube 28, the freezing tube 28 is inserted into the placing groove 16 from a cross cut 18 on the anti-shaking ring 17, the bottom of the freezing tube 28 is contacted with the rubber shock pad 14, because the anti-shaking ring 17 has elasticity, the upper part of the anti-shaking ring 17 becomes a four-flap structure of downward inclination under the extrusion of the freezing tube 28, the four-flap structure encloses a cone, the freezing tube 28 is wrapped in the cone structure, the cone structure extrudes the outer wall of the freezing tube 28 under the action of self resilience force, thereby fixing the upper part of the freezing tube 28, because the freezing tube 28 is not inserted into the placing groove 16, the distance between the two clamping plates 23 is smaller than the outer diameter of the freezing tube 28, when freezing and depositing pipe 28 and insert standing groove 16, freezing and depositing pipe 28 extrudees splint 23 and makes guide bar 24 move to the direction that leaves splint 23 away from to extrude compression spring 25, compression spring 25 can exert resilience force to splint 23, makes splint 23 press from both sides the tight tube 28 that freezes of depositing, avoids freezing and deposits the tube 28 and rock and cause the damage.

The above-mentioned embodiment is right the utility model discloses an explanation, it is not right the utility model discloses a limited, any right the scheme after the simple transform of the utility model all belongs to the protection scope of the utility model.

Claims

1. An immune cell preservation device, characterized in that: the box cover comprises a box body and a box cover arranged at the top of the box body and used for sealing the box body, wherein one side of the box cover is hinged to the box body, the other side of the box cover is detachably and fixedly connected with the box body, a placing table used for placing a cryopreservation pipe is arranged inside the box body, the bottom of the placing table is detachably and fixedly connected to the inner wall of the bottom of the box body, a plurality of placing grooves with upward openings and used for placing the cryopreservation pipe are formed in the placing table, the placing grooves are vertically arranged inside the placing table and extend towards the lower part of the placing table, a shaking-proof moving coil is fixedly connected to the inner wall of the upper part of the placing groove, a cross-shaped notch is formed in the middle of the shaking-proof moving coil, and a plurality of clamping mechanisms.

2. The immune cell preservation device of claim 1, wherein: clamping mechanism sets up the splint that are used for pressing from both sides tight cryopreserving pipe inside the standing groove including the symmetry, place the inside guide way that is the font that sets up of symmetry, sliding connection has the guide bar in the guide way, the one end of guide bar is located the guide way, the other end of guide bar extend to the standing groove in and with splint fixed connection, the guide bar periphery cover is equipped with compression spring, compression spring's one end and splint fixed connection, compression spring's the other end and the inner wall fixed connection of standing groove, the stopper that is used for preventing the guide bar from breaking away from inside the guide way that is located two symmetries of fixedly connected with setting on the guide bar of inside of guide way.

3. The immune cell preservation device of claim 2, wherein: four angle departments fixedly connected with longitudinal section of placing the platform bottom are the fixture block of T shape, fixedly connected with on the inner wall of bottom half with the mount pad of fixture block one-to-one, set up in the mount pad with fixture block matched with draw-in groove, place the platform and pass through the fixture block can dismantle fixed connection on the inner wall of bottom half.

4. The immune cell preservation device of claim 3, wherein: the longitudinal section of mount pad is trapezoidal, equal first damping spring of fixedly connected with on two sides about the fixture block, first damping spring deviates from the one end of fixture block and the inner wall fixed connection of mount pad, has all laid the rubber shock pad on the bottom surface of mount pad inside and the bottom of standing groove.

5. The immune cell preservation device of claim 2, wherein: and a second damping spring is fixedly connected between the periphery of the outside of the placing table and the inner side wall of the box body.

6. The immune cell preservation device of claim 2, wherein: the placing table is cylindrical, the placing grooves are divided into three groups, the placing table comprises a small hole groove group, a middle hole groove group and a large hole groove group which are sequentially arranged from the middle position of the placing table to the edge of the placing table, the small hole groove group comprises a plurality of small hole grooves, the middle hole groove group comprises a plurality of middle hole grooves, the large hole groove group comprises a plurality of large hole grooves, the inner diameter of each middle hole groove is larger than the inner diameter of each small hole groove and smaller than the inner diameter of each large hole groove, the depth of each middle hole groove is larger than the depth of each small hole groove and smaller than the depth of each large hole groove, and the placing grooves of each group are uniformly arranged along the circumferential direction.

7. The immune cell preservation device of any one of claims 1-6 wherein: the box body is of a composite layer structure and comprises a heat preservation layer, a buffer layer and a waterproof layer which are sequentially arranged from inside to outside.

8. The immune cell preservation device of any one of claims 1-6 wherein: the anti-shaking ring is made of an elastic material.

9. The immune cell preservation device of any one of claims 1-6 wherein: the top of the box cover is fixedly connected with a lifting handle.

10. An immune cell preservation device according to any one of claims 3 to 6 wherein: the limiting blocks and the clamping blocks are made of elastic rubber.