CN212232648U - Detachable rodent social ability behavior detection device - Google Patents
Detachable rodent social ability behavior detection device Download PDFInfo
- Publication number
- CN212232648U CN212232648U CN202020826334.5U CN202020826334U CN212232648U CN 212232648 U CN212232648 U CN 212232648U CN 202020826334 U CN202020826334 U CN 202020826334U CN 212232648 U CN212232648 U CN 212232648U
- Authority
- CN
- China
- Prior art keywords
- detachable
- detection device
- behavior detection
- rodent
- plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a detachable rodent social ability behavior detection device, which comprises a bottom plate and a side plate, wherein the bottom plate is provided with a plurality of grooves, and the side plate is inserted into the grooves to form a middle cavity and two side chamber cavities; and a baffle is arranged between the middle cavity and the two side chamber cavities and is inserted into the groove on the bottom plate, so that a concave canyon type channel is formed between the middle cavity and the two side chamber cavities. The device is through piecing together the connected mode of inserting the gomphosis, easily adopts suitable connected mode in order to adapt to specific experimental space requirement according to multiple action experiment requirement, pieces together promptly and uses, and the flexibility is high, and convenient operation saves space, is convenient for deposit.
Description
Technical Field
The utility model relates to a detachable rodent social ability action detection device belongs to animal action test technical field.
Background
The three-box social experiment is a mature experimental device for detecting the social behaviors of rodents at present. The principle is based on the characteristic that mice naturally love the social population and have exploration tendency on new objects. Most current three-box social behavior devices are composed of three rectangular boxes, the specification of each box is 27cm X45 cm, a separation plate between each box is made of organic glass, and a 5cm X5 cm channel is arranged in the middle of each box to enable the three boxes to be communicated. The device simple manufacture, though can accomplish the behavioural experiment, can not satisfy the demand of surveying the electrophysiology simultaneously. The classical electrophysiological recording is to implant electrodes in the mouse brain and directly detect the corresponding brain area electrical signals. The procedure requires the electrodes to be connected to the neural signal processor by a headstage front-end amplifier, in other words, the electrodes on the mouse brain bag are connected to the computer by a wire. Obviously, the electrophysiological laboratory mouse could not move freely in the three chambers because there was only one small 5cm by 5cm hole between the central chamber and the side chamber of the conventional three chambers. Some laboratories convert the traditional three boxes into linear three boxes, only allow the laboratory mouse to move in the central room, and the device is compatible with electrophysiology, but still has the defects of large floor area, inconvenience in storage and carrying, smell interference of glue and the like.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that overcome prior art's defect, provide a detachable rodent social ability action detection device, connect through the mode of piecing together the gomphosis of inserting, reach the detachable effect.
In order to solve the technical problem, the utility model provides a detachable rodent social ability behavior detection device, which comprises a bottom plate and a side plate, wherein the bottom plate is provided with a plurality of grooves, and the side plate is inserted into the grooves to form a middle cavity and two side chamber cavities; and a baffle is arranged between the middle cavity and the two side chamber cavities and is inserted into the groove on the bottom plate, so that a concave canyon type channel is formed between the middle cavity and the two side chamber cavities.
Preferably, the baffle is transparent plexiglass.
Preferably, the baffle is provided with four blocks, wherein the top right angles of the two baffles are ground into circular arcs.
Preferably, the bottom plate is formed by mutually splicing a first bottom plate and a second bottom plate.
Preferably, the side plates comprise two first side plates and two second side plates, the two first side plates are arranged in parallel, and the two second side plates are respectively arranged perpendicular to the first side plates.
Preferably, the upper portion of the first side plate is provided with a plurality of clamping grooves, the upper portions of the second side plate and the baffle are provided with clamping blocks, and the clamping blocks are respectively inserted into the clamping grooves in the upper portion of the first side plate.
Preferably, the bottoms of the first side plate, the second side plate and the baffle are respectively provided with a protrusion matched with the groove on the bottom plate, and the protrusion is inserted into the groove on the bottom plate.
Preferably, a metal cage for limiting the moving range of the rodent is arranged in the side chamber cavity, and a cylindrical kettle filled with water is arranged above the metal cage.
The utility model discloses the beneficial effect who reaches:
(1) the device is through piecing together the connected mode of inserting the gomphosis, easily adopts suitable connected mode in order to adapt to specific experimental space requirement according to multiple action experiment requirement, pieces together promptly and uses, and the flexibility is high, and convenient operation saves space, is convenient for deposit.
(2) An unsealed concave canyon type channel is formed between the middle cavity and the two side cavity, wired electric signal records can be allowed to pass through the channel, the function of the device for detecting the animal social behaviors and the electroencephalogram physiological signals simultaneously is realized, and the intracerebral electric signal activities of the mice can be monitored in real time in the social process of the mice.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic structural diagram of the bottom plate of the present invention;
fig. 3 is a schematic structural view of the first side plate of the present invention;
fig. 4 is a schematic structural view of a second side plate of the present invention;
fig. 5 is a schematic structural diagram of the baffle of the present invention.
Detailed Description
The present invention will be further described with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
As shown in fig. 1, a detachable rodent social ability behavior detection device comprises a bottom plate and a side plate, wherein a plurality of grooves 1 are formed in the bottom plate, the side plate is inserted into the grooves 1 to form a middle cavity 2 (25 cm × 48cm × 40 cm) and two side chamber cavities 3 (20 cm × 48cm × 40 cm); a baffle 4 is arranged between the middle cavity 2 and the two side chamber cavities 3, and the baffle 4 is inserted into the groove 1 on the bottom plate, so that a concave canyon type channel is formed between the middle cavity 2 and the two side chamber cavities 3. The device can allow a mouse with an embedded electrode in the brain to freely move in the device after being connected with the wire, and the normal record of electrophysiological signals cannot be influenced.
As shown in fig. 2 to 5, the baffle 4 is four pieces of transparent organic glass. In order to avoid the influence on the normal movement of animals caused by the winding or knotting of the wires for recording signals, the top right angles of the two baffles 4 are ground into circular arcs, so that the possibility of interference on the movement of mice is greatly reduced. The bottom plate is formed by mutually splicing a first bottom plate 5 and a second bottom plate 6. The curb plate includes two first curb plates 7 and two second curb plates 8, and two first curb plates 7 parallel arrangement, two second curb plates 8 set up with first curb plate 7 perpendicular respectively. The upper portion of the first side plate 7 is provided with a plurality of clamping grooves 9, the upper portions of the second side plate 8 and the baffle plate 4 are provided with clamping blocks 10, and the clamping blocks 10 are respectively inserted into the clamping grooves 9 in the upper portion of the first side plate 7. The bottom of the first side plate 7, the bottom of the second side plate 8 and the bottom of the baffle 4 are respectively provided with a protrusion 11 matched with the groove 1 on the bottom plate, and the protrusion 11 is inserted into the groove 1 on the bottom plate. As shown in fig. 2, the positions of the two first side plates 7, the two second side plates 8 and the four baffle plates 4 inserted into the bottom plate groove 1 are respectively E, C, D.
In the experiment, the test mouse was allowed to enter the lateral chamber cavity 3 and directly contact the social mouse in the metal cage. In the side chamber, the metal cage is a metal pen container with the diameter of 9cm and the height of 10.5cm, the metal pen container is reversely buckled with a social mouse to limit the moving range of the social mouse, and a cylindrical kettle filled with water is placed on the pen container to be pressed and fixed.
The specific experimental process is as follows:
a) before the experiment begins, the mouse is placed in a behavior detection device to adapt for half an hour;
b) placing the test mouse into the middle cavity 2 to adapt for 5 minutes;
c) turning on the camera device and recording the relevant parameters
d) The experiment is divided into two stages, in the first stage, strange rats (marked as S1) are randomly placed in the metal cages of the left side chamber cavity 3 or the right side chamber cavity 3, the metal cage of the other side chamber cavity is empty, and the record is carried out for 10 min; the second stage is to place a second strange mouse of the same species (labeled as S2) in a metal cage with the other side of the cage empty, and record the same for 10 min.
When electrophysiological data is acquired, the electrode at the head of the mouse is connected with a multichannel neural signal recording system, and parameters are set to record electrophysiological data for 30min under the condition that the mouse is awake and quiet. And repeating the performed ethology, collecting the electrophysiological data in the whole process and recording the video to obtain the electrophysiological data combined with the ethology.
And (3) data analysis: the behavioral data are expressed as mean + -SEM and statistically analyzed using the one-way ANOVA and Tukey test in GraphPad Prism 7.00. The behavior was recorded by the camera and the sniff time was analyzed uniformly by the anymaze software. Electrophysiological data were analyzed by classification of single cells using the offline sorter, and analysis of continuous signals using spike2 and neuroexplore ev 5. And for the electrophysiological data combined with the behavioristics, the video is analyzed to divide the experimental mouse into time segments of different behaviors, and the electrophysiological data is analyzed according to the time segments to compare the difference of corresponding brain area electric signals of the experimental mouse in different behaviors.
The relevant parameters include:
1. testing the contact frequency and the contact duration of the rat and strange rats S1 and S2 or an empty metal cage, wherein the contact range is defined as 3-5 cm around the metal cage;
2. the number of times and duration of entry of the mouse into each chamber was tested and when both the head and four paws of the mouse entered one chamber it was considered to be in that chamber.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be considered as the protection scope of the present invention.
Claims (8)
1. The detachable rodent social ability behavior detection device is characterized by comprising a bottom plate and side plates, wherein a plurality of grooves are formed in the bottom plate, and the side plates are inserted into the grooves to form a middle cavity and two side chamber cavities; and a baffle is arranged between the middle cavity and the two side chamber cavities and is inserted into the groove on the bottom plate, so that a concave canyon type channel is formed between the middle cavity and the two side chamber cavities.
2. The detachable rodent sociability behavior detection device of claim 1, wherein the barrier is clear perspex.
3. The detachable rodent social ability behavior detection device of claim 1, wherein the baffles are four, wherein the top right angles of the two baffles are ground into a circular arc.
4. The detachable rodent social performance behavior detection device of claim 1, wherein the base plate is formed by a first base plate and a second base plate being joined to one another.
5. The detachable rodent social ability behavior detection device of claim 1, wherein the side plates comprise two first side plates and two second side plates, the two first side plates being arranged in parallel and the two second side plates being arranged perpendicular to the first side plates, respectively.
6. The detachable rodent social ability behavior detection device according to claim 5, wherein the first side plate is provided with a plurality of slots at an upper portion thereof, and the second side plate and the baffle are provided with blocks at upper portions thereof, and the blocks are respectively inserted into the slots at the upper portion of the first side plate.
7. The detachable rodent social ability behavior detection device of claim 5, wherein the bottom of the first side plate, the bottom of the second side plate and the bottom of the baffle are respectively provided with a protrusion which is matched with the groove on the bottom plate, and the protrusions are inserted into the grooves on the bottom plate.
8. The detachable rodent social ability behavior detection device according to claim 1, wherein a metal cage limiting the rodent range of motion is arranged in the side chamber cavity, and a cylindrical kettle filled with water is arranged above the metal cage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020826334.5U CN212232648U (en) | 2020-05-18 | 2020-05-18 | Detachable rodent social ability behavior detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020826334.5U CN212232648U (en) | 2020-05-18 | 2020-05-18 | Detachable rodent social ability behavior detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212232648U true CN212232648U (en) | 2020-12-29 |
Family
ID=74001001
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020826334.5U Active CN212232648U (en) | 2020-05-18 | 2020-05-18 | Detachable rodent social ability behavior detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212232648U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113142146A (en) * | 2021-05-14 | 2021-07-23 | 云南大学 | Device and method for establishing insect social barrier model |
| WO2023102797A1 (en) * | 2021-12-09 | 2023-06-15 | 中国科学院深圳先进技术研究院 | Electrode protection device |
-
2020
- 2020-05-18 CN CN202020826334.5U patent/CN212232648U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113142146A (en) * | 2021-05-14 | 2021-07-23 | 云南大学 | Device and method for establishing insect social barrier model |
| CN113142146B (en) * | 2021-05-14 | 2023-08-18 | 云南大学 | Device and method for establishing insect social obstacle model |
| WO2023102797A1 (en) * | 2021-12-09 | 2023-06-15 | 中国科学院深圳先进技术研究院 | Electrode protection device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN212232648U (en) | Detachable rodent social ability behavior detection device | |
| Griffin et al. | Spatial representations of hippocampal CA1 neurons are modulated by behavioral context in a hippocampus-dependent memory task | |
| Komorowski et al. | Robust conjunctive item–place coding by hippocampal neurons parallels learning what happens where | |
| Nakazawa et al. | Hippocampal CA3 NMDA receptors are crucial for memory acquisition of one-time experience | |
| CA2573049C (en) | Honey bee acoustic recording and analysis system for monitoring hive health | |
| Fiscella et al. | Recording from defined populations of retinal ganglion cells using a high-density CMOS-integrated microelectrode array with real-time switchable electrode selection | |
| Chang et al. | Neurophysiological correlates of object recognition in the dorsal subiculum | |
| CN109566428B (en) | Experimental device and experimental method for animal instinct fear behavior | |
| Titiz et al. | Cognitive impairment in temporal lobe epilepsy: role of online and offline processing of single cell information | |
| Troisi et al. | Environmental and social influences on autogrooming behaviour in a captive group of Java monkeys | |
| Honda-Sumi | Difference in calling song of three field crickets of the genus Teleogryllus: the role in premating isolation | |
| Plakke et al. | Coding of vocalizations by single neurons in ventrolateral prefrontal cortex | |
| CN109588341A (en) | A kind of dedicated rodent conditioned fear reaction detection cage box of behaviouristics detection | |
| Alisch et al. | MAPLE (modular automated platform for large-scale experiments), a robot for integrated organism-handling and phenotyping | |
| Bartlett et al. | The effect of new recruits on the flock specific call of budgerigars (Melopsittacus undulatus) | |
| Garcia-Cairasco et al. | Neuroethological study of status epilepticus induced by systemic pilocarpine in Wistar audiogenic rats (WAR strain) | |
| Hom et al. | Echolocation behavior in big brown bats is not impaired after intense broadband noise exposures | |
| Kotila et al. | Large‐scale long‐term passive‐acoustic monitoring reveals spatio‐temporal activity patterns of boreal bats | |
| Todt et al. | The vocal behavior of Barbary macaques (Macaca sylvanus): Call features and their performance in infants and adults | |
| CN212232649U (en) | Rodent social ability behavior detection device | |
| Bick et al. | Dynamics of pollen beetle (Brassicogethes aeneus) immigration and colonization of oilseed rape (Brassica napus) in Europe | |
| da Silva Guerra et al. | Semi-automatic behavior analysis using robot/insect mixed society and video tracking | |
| Gary et al. | Tracheal mite (Acari: Tarsonemidae) infestation effects on foraging and survivorship of honey bees (Hymenoptera: Apidae) | |
| Kim et al. | Olfactory discrimination ability of South African fur seals (Arctocephalus pusillus) for enantiomers | |
| CN113142145B (en) | Device and method for observing social habits among insect individuals |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |