CN212213708U - Endoscopic probe and imaging system suitable for two-photon fluorescence imaging - Google Patents
Endoscopic probe and imaging system suitable for two-photon fluorescence imaging Download PDFInfo
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- CN212213708U CN212213708U CN201922218344.0U CN201922218344U CN212213708U CN 212213708 U CN212213708 U CN 212213708U CN 201922218344 U CN201922218344 U CN 201922218344U CN 212213708 U CN212213708 U CN 212213708U
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Abstract
The utility model discloses an peep probe and imaging system in suitable for two-photon fluorescence formation of image. The endoscopic probe comprises a double-envelope optical fiber, a first micro lens, an MEMS micro mirror and a second micro lens which are sequentially arranged along an incident light path; the double-envelope optical fiber comprises a fiber core, a first envelope layer and a second envelope layer which are sequentially arranged from inside to outside, wherein the fiber core is used for transmitting femtosecond laser, and the first envelope layer is used for transmitting two-photon fluorescence generated by a sample. The system comprises the endoscopic probe, the femtosecond laser, a first lens, a plane reflector, a plane dichroic reflector, a second lens, a third lens and a detector. The utility model adopts the double-envelope optical fiber to overcome the difficulty that the prior art adopts a double-path optical fiber to respectively transmit femtosecond laser and collect fluorescent signals, which can not further reduce the size; by adopting a flexible design scheme of MEMS micro-mirror scanning, the limitation that the PZT driven optical fiber scanning can only realize forward scanning is overcome.
Description
Technical Field
The utility model relates to a two-photon endoscopic microscopic imaging field, in particular to an endoscopic probe and an imaging system suitable for two-photon fluorescence imaging.
Background
Two-photon imaging was first reported in 1990 by doctor's Denk, professor Webb, and is a nonlinear optical imaging method. Since two-photon imaging utilizes near-infrared light with low scattering and absorption in biological tissues, this technique has the advantages of low photobleaching and phototoxicity, superior tissue penetration, resolution at the sub-cellular level, and inherent chromatographic power, thus providing a powerful tool for label-free, in vivo imaging. In recent years, two-photon imaging technology has been greatly developed in many aspects, and not only has great success in meeting new requirements of neuroscience research, but also has a rapid development trend in clinical application research.
In the field of brain neuroscience, researchers hopefully observe brain nerve cells in a natural physiological state for a long time with cell resolution, and effectively record nerve network activities of animals in a waking free behavior state. Therefore, to better meet the demand of neuroscience research, two-photon imaging devices are currently developing towards miniaturization and portability of animal heads. Such as a 2.15 gram miniature two-photon microscope developed by the Beijing university program and academician team. The miniature two-photon microscope consists of an electrostatic driving MEMS scanner and a high-digital-aperture miniature objective lens, wherein laser and fluorescence transmission respectively use one optical fiber and are carried on the head of a mouse, and clear images of dynamic neural network movement are obtained when the mouse freely moves.
In clinical applications, researchers hope more that two-photon fluorescence imaging of endogenous (NADH and FAD) optical markers can be used to diagnose various tumor diseases such as digestive tract tumors, brain tumors, and skin cancers. However, the application research of two-photon fluorescence imaging in tumor diagnosis is mainly carried out on isolated or living small animal tissues, and a long way is left from the real clinical application. Therefore, the development of endoscopic two-photon imaging technology will be a key step for realizing noninvasive, in-vivo and in-situ tumor diagnosis. Meanwhile, the development of the desktop two-photon imaging technology into the endoscopic two-photon imaging technology is also clarified in the ' objective lens group for two-photon fluorescence endoscope ', and the utility model CN201810015806.6 ', which will greatly promote the application of the two-photon imaging technology in the medical fields of tumor detection, cancer and the like in the operation. For example, the Li Xingde team at the university of John Hopkins [ Light: Science & Applications (2017)6, e17082 ] in the U.S. provides a two-photon endoscope based on a piezoelectric ceramic tube (PZT) driven forward scanning fiber, using endogenous (NADH and FAD) optical markers, in vivo two-photon fluorescence imaging of an unlabeled mouse renal ischemia reperfusion model. The Lixing de group indicates that on one hand, the two-photon endoscopic probe can realize in-vivo, in-situ and real-time pathological imaging on a lumen organ, and opens up a new path for disease diagnosis in clinic; on the other hand, the two-photon endoscopic probe can be used as a powerful tool for basic research of brain functions, is carried on the head of a freely moving animal, and acquires the dynamic neural network moving process with high resolution.
In summary, in both the basic research field of brain science and the clinical disease diagnosis field, the development of the endoscopic two-photon imaging technology will be a trend that cannot be blocked in the development process of the future two-photon imaging technology. According to different scanning modes, the endoscopic probe has two modes, namely MEMS (Micro-Electro-mechanical systems) Micro-mirror scanning (Oaku Ohio and Oaku Ching Co., Ltd.) and PZT (PZT) driven optical fiber scanning (Lixing Co Ltd.). PZT fiber scanning is suitable for forward scanning; the MEMS micro-reflector has flexible scanning design, and can realize forward scanning and lateral scanning through a simple optical element turning optical fiber. At present, the reported MEMS micro-mirror scanning endoscopic probe applied in the two-photon imaging system adopts a structural design of separating the excitation light from the signal light, and the size and structure of the probe are relatively large, which is not favorable for further development to endoscopic imaging.
SUMMERY OF THE UTILITY MODEL
The technical problem to be solved in the present invention is to provide an endoscopic probe and an imaging system suitable for two-photon fluorescence imaging, which are not enough in the prior art.
In order to solve the technical problem, the utility model discloses a technical scheme is: an endoscopic probe suitable for two-photon fluorescence imaging comprises a double-envelope optical fiber, a first micro lens, an MEMS micro mirror and a second micro lens which are sequentially arranged along an incident light path;
the femtosecond laser emitted by the double-envelope optical fiber is collimated by the first micro lens and reflected by the MEMS micro mirror in sequence and then converged on a sample by the second micro lens; two-photon fluorescence generated by the sample is collected by the second micro lens and then is converged into the double-envelope optical fiber by the first micro lens after being reflected by the MEMS micro mirror;
the double-envelope optical fiber comprises a fiber core, a first envelope layer and a second envelope layer which are sequentially arranged from inside to outside, wherein the fiber core is used for transmitting femtosecond laser, and the first envelope layer is used for transmitting two-photon fluorescence generated by a sample.
Preferably, the core and the first cladding have different refractive indices.
Preferably, the double-envelope optical fiber is coaxial with the first microlens;
the plane of the MEMS micro-mirror and the optical axes of the first micro-lens and the second micro-lens form an included angle of 45 degrees, and the center of the MEMS micro-mirror is located at the intersection point of the optical axes of the first micro-lens and the second micro-lens.
Preferably, the device further comprises a probe mounting part, wherein the probe mounting part comprises a shell, and a fiber support, a micro lens support and a micro mirror support which are arranged in the shell.
Preferably, the middle part of the optical fiber bracket is provided with an optical fiber hole for arranging the double-envelope optical fiber;
the bottom of the micro lens support is provided with an installation groove, the side surface of the micro lens support is provided with a first lens hole and a second lens hole which are perpendicular to each other and are communicated with the installation groove, the first micro lens is arranged in the first lens hole, and the second micro lens is arranged in the second lens hole;
the micro lens support is arranged on the side part of the optical fiber support, and the optical fiber hole is communicated with the first lens hole and shares a central axis;
the micro-mirror support is arranged in the mounting groove and is provided with a 45-degree inclined plane for arranging the MEMS micro-mirror.
Preferably, the housing is cylindrical, and a window through which the femtosecond laser and the two-photon fluorescence pass is formed above the second microlens on the housing.
Preferably, the middle part of the optical fiber support is further provided with a tapered hole, a first end of the tapered hole is communicated with the optical fiber hole, and a second end of the tapered hole is communicated with the first lens hole; the diameter of the first end of the tapered hole is equal to the diameter of the optical fiber hole, and the diameter of the second end of the tapered hole is equal to the diameter of the first lens hole.
The utility model also provides an imaging system suitable for two-photon fluorescence imaging, including peeping the probe as above.
Preferably, the laser further comprises a femtosecond laser, a first lens, a plane mirror, a plane dichroic mirror, a second lens, a third lens and a detector.
Preferably, the femtosecond laser emitted by the femtosecond laser device is collimated by the first lens, then reflected by the plane reflector and the plane dichroic reflector in sequence, and then converged into the double-light envelope layer optical fiber by the second lens, the double-light envelope layer optical fiber guides the femtosecond laser into the endoscopic probe, and the endoscopic probe scans a sample to be detected; two-photon fluorescence generated after a sample is excited by femtosecond laser is guided into the two-photon enveloping layer optical fiber through the endoscopic probe, the two-photon fluorescence emitted by the two-photon enveloping layer optical fiber is collimated by the second lens, then transmits the plane dichroic reflector, and then is converged by the third lens and then enters the detector.
The utility model has the advantages that: the utility model adopts the double-envelope optical fiber to overcome the difficulty that the prior art adopts a double-path optical fiber to respectively transmit femtosecond laser and collect fluorescent signals, which can not further reduce the size; the flexible design scheme of MEMS micro-mirror scanning is adopted, so that the limitation that the PZT driving optical fiber scanning can only realize forward scanning is overcome; the endoscopic probe of the utility model can realize the scanning view field size of 450 mu m multiplied by 450 mu m, and compared with the scanning view field of the existing miniature two-photon probe of the electrostatic driving MEMS micro-reflector for brain science research, the scanning view field is improved by 12 times; compared with the optical fiber scanning endoscopic probe driven by PZT, the size of the field of view is improved by 26 times.
Drawings
Fig. 1 is a schematic structural diagram of an imaging system suitable for two-photon fluorescence imaging in embodiment 2 of the present invention;
fig. 2 is a schematic structural diagram of an endoscopic probe suitable for two-photon fluorescence imaging according to embodiment 1 of the present invention;
fig. 3 is a schematic structural view of a probe mounting member according to embodiment 1 of the present invention;
fig. 4 is an end face structure view of a double clad optical fiber according to embodiment 1 of the present invention;
fig. 5 is an optical configuration diagram of an endoscopic probe according to embodiment 1 of the present invention;
fig. 6 is an optical configuration diagram of a forward scanning endoscopic probe according to embodiment 1 of the present invention.
Description of reference numerals:
10-a femtosecond laser, 11-a first lens, 12-a plane reflector, 13-a plane dichroic reflector, 14-a second lens, 15-a third lens, 16-a double-envelope optical fiber, 17-a detector, 160-a fiber core, 161-a first envelope and 162-a second envelope;
2-endoscopic probe, 20-first microlens, 21-second microlens, 22-MEMS micro-mirror, 23-flexible electrode, 24-first cemented lens, 25-second cemented lens, 26-reflector;
3-a biological sample;
4-probe mounting; 40, a shell; 41-optical fiber support, 42-microlens support, 43-micromirror support.
Detailed Description
The present invention is further described in detail below with reference to examples so that those skilled in the art can implement the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
As shown in fig. 2, an endoscopic probe 2 suitable for two-photon fluorescence imaging according to the present embodiment includes a double-clad optical fiber 16, a first micro lens 20, a MEMS micro-mirror 22, and a second micro lens 21, which are sequentially disposed along an incident light path;
the femtosecond laser emitted by the double-envelope layer optical fiber 16 is collimated by the first micro lens 20 and reflected by the MEMS micro mirror 22 in sequence and then converged on the biological sample 3 by the second micro lens 21; the two-photon fluorescence generated by the biological sample 3 is collected by the second micro lens 21, reflected by the MEMS micro mirror 22, converged by the first micro lens 20, and then coupled into the double-envelope layer optical fiber 16; the double-envelope optical fiber 16 is transmitted outwards again for fluorescent signal detection.
The double-cladding optical fiber 16 comprises a fiber core 160, a first cladding 161 and a second cladding 162 which are sequentially arranged from inside to outside, wherein the fiber core 160 and the first cladding 161 have different refractive indexes, the fiber core 160 is used for transmitting femtosecond laser, and the first cladding 161 is used for transmitting two-photon fluorescence generated by a sample. The refractive index difference between the first cladding 161 and the core 160 determines that only single-mode laser light can be transmitted in the core 160, i.e. single-mode femtosecond laser light can be transmitted in the core 160; while the difference in refractive index between the first and second coatings 161, 162 determines that a multi-mode fluorescence signal is transmitted in the first coating 161, i.e. the two-photon fluorescence signal generated by the biological sample 3 is collected by the first coating 161. The double clad fiber 16 preferably used mainly includes two types, one is a pure fiber structure and the other is a photonic crystal structure. The present invention is further described by the following embodiments based on the general concept of the present invention.
Example 1
Referring to fig. 2-5, in the present embodiment, the dual-clad optical fiber 16 and the first microlens 20 have the same optical axis; the plane of the MEMS micro-mirror 22 and the optical axes of the first micro-lens 20 and the second micro-lens 21 form an included angle of 45 °, and the center of the MEMS micro-mirror 22 is located at the intersection point of the optical axes of the first micro-lens 20 and the second micro-lens 21.
The MEMS micro-mirror 22 can be driven by four driving methods, such as electrothermal, electromagnetic, electrostatic and piezoelectric, so as to achieve the volume miniaturization of the endoscopic probe 2, and the MEMS micro-mirror 22 driven by electrothermal is preferably selected. For example, the MEMS micro-mirror can be electrically driven by tin-free micro-optics provided by the company of the uko, and the performance parameters of the MEMS micro-mirror are shown in table 1.
In the present embodiment, the probe mounting device 4 is further included, and the probe mounting device 4 includes a housing 40, and a fiber holder 41, a microlens holder 42, and a micromirror holder 43 disposed in the housing 40.
Wherein, the middle part of the optical fiber bracket is provided with an optical fiber hole for arranging the double-envelope optical fiber 16; the bottom of the micro-lens support 42 is provided with a mounting groove, the side surface of the micro-lens support 42 is provided with a first lens 11 hole and a second lens 14 hole which are perpendicular to each other and are communicated with the mounting groove, the first micro-lens 20 is arranged in the first lens 11 hole, and the second micro-lens 21 is arranged in the second lens 14 hole; thereby ensuring that the optical axes of the first microlens 20 and the second microlens 21 are perpendicular to each other;
the micro-lens support 42 is arranged at the side part of the optical fiber support 41, and the optical fiber hole is communicated with the first lens 11 hole and shares a central axis; to ensure that the optical axes of the double-envelope fiber 16 and the first microlens 20 are coaxial. Further, the middle part of the optical fiber bracket 41 is also provided with a tapered hole, the first end of which is communicated with the optical fiber hole and the second end of which is communicated with the first lens 11 hole; the diameter of the first end of the tapered hole is equal to the diameter of the fiber hole and the diameter of the second end of the tapered hole is equal to the diameter of the first lens 11 hole.
The micro-mirror bracket 43 is arranged in the mounting groove, and the micro-mirror bracket 43 is provided with a 45-degree inclined plane for arranging the MEMS micro-mirror 22; the plane of the MEMS micro-mirror 22 is ensured to form an angle of 45 degrees with the optical axes of the first micro-lens 20 and the second micro-lens 21, and meanwhile, the center of the MEMS micro-mirror 22 is located at the intersection point of the optical axes of the first micro-lens 20 and the second micro-lens 21. Further, a flexible electrode 23 is positioned between the MEMS micro-mirror 22 and the micro-mirror support 43 so as to introduce an external control signal to the electrode of the MEMS micro-mirror 22 for controlling the rotation of the MEMS micro-mirror 22.
The housing 40 is cylindrical, and a window through which the femtosecond laser and the two-photon fluorescence pass is formed above the second microlens 21 on the housing 40.
In a further preferred embodiment, the specific optical parameters of the endoscopic probe 2 are also designed.
According to the document [ Nature Biotechnology,21(11): 1369-.
ωxyIs representative of the lateral resolution, ωzRepresenting the axial resolution, NA is the numerical aperture of the image space. In the optical design of the MEMS scanning endoscopic probe 2, a telecentric optical path design scheme is adopted. The femtosecond laser from the double-envelope fiber 16 is collimated into parallel light by the first microlens 20, and then the parallel light is converged by the second microlens 21 and then incident on the biological sample 3. The MEMS micro-mirror 22 is located in a parallel optical path between the two micro-lenses and in the focal position of the second micro-lens 21 in order to reduce the non-linearity of the scan. The focusing power of the MEMS scanning endoscopic probe 2 is determined by the NA of the double clad fiber 16, the effective focal length of the two microlenses, and the size of the MEMS micro-mirror.
In the present embodiment, the mirror sizes of the MEMS micro-mirrors 22 are 0.52mm × 0.52 mm; the first micro lens 20 is an aspheric lens 354140-B of Thorlabs, the lens material is D-ZK7, the diameter is 1 mm; the second microlens 21 was made of Edmund' S achromatic double cemented lens NT65-564, and the first and second cemented lenses 24 and 25 of the second microlens 21 were made of S-PHM52 and N-LASF9, respectively, and had a diameter of 2.4 mm. Considering the factors of minimizing the volume of the whole endoscopic probe 2, packaging and fixing the MEMS micro-mirror 22, and the like, the final optimization determines that the optical structure of the MEMS micro-mirror scanning endoscopic probe 2 is shown in fig. 5, and the structural parameters of the optical system are shown in table 2.
In this embodiment, when the mechanical rotation angle of the MEMS micro-mirror 22 is ± 5 degrees, the size of the field of view scanned by the MEMS micro-mirror 22 on the focal plane of the scanning endoscopic probe 2 is about 450 μm × 450 μm, which is about 12 times higher than the field of view (130 μm × 130 μm) of the MEMS scanning dual-channel micro two-photon probe based on electrostatic driving reported so far, and about 25 times higher than the PZT driving optical fiber scanning (diameter about 100 μm) for clinical disease diagnosis. In this embodiment, the image-side numerical aperture NA is about 0.1, and the equation (1) is substituted, so that the actual lateral resolution of the central position of the endoscopic probe 2 provided by the present invention is calculated to be about 2 μm, and the wavelength is 920 nm. The focal position is located about 774 μm behind the second microlens 21. The diameter of the whole endoscopic probe 2 can be controlled within 3.5 mm.
Referring to fig. 6, an optical structure of the endoscopic probe 2 in another embodiment is shown. It is improved on the basis of side scanning, and a simple mirror 26 is added between the first micro-mirror and the MEMS micro-mirror 22, so that the forward scanning can be conveniently realized, and the whole optical performance is consistent with the side scanning performance. Compared with lateral scanning, the size of the forward scanning two-photon endoscopic probe 2 is increased to a certain extent, and the diameter can be controlled within 4.5 mm.
The utility model provides a two-photon endoscopic probe 2 based on MEMS micro-reflector 22 scanning, which adopts the double-envelope optical fiber 16 to overcome the difficulty that the prior art adopts double-path optical fibers to respectively transmit femtosecond laser and collect fluorescent signals, which can not further reduce the size; the flexible design scheme of scanning by the MEMS micro-mirror 22 is adopted, so that the limitation that forward scanning can only be realized by PZT driven optical fiber scanning is overcome. The two-photon endoscopic probe 2 based on the scanning of the MEMS micro-reflector 22 in the utility model can realize the scanning view field size of 450 mu m multiplied by 450 mu m, and compared with the existing micro two-photon probe used for the electrostatic driving MEMS micro-reflector 22 for brain science research, the scanning view field is improved by 12 times; compared with the optical fiber scanning endoscopic probe 2 for PZT driving, the size of the field of view is improved by 26 times.
Example 2
Referring to fig. 1, an imaging system suitable for two-photon fluorescence imaging includes a femtosecond laser 1, a first lens 11, a plane mirror 12, a plane dichroic mirror 13, a second lens 14, a third lens 15, a detector 17, and the endoscopic probe 2 in embodiment 1.
The femtosecond laser emitted by the femtosecond laser 1 is collimated by the first lens 11, then reflected by the plane reflector 12 and the plane dichroic reflector 13 in sequence, and then converged into the double-light envelope layer optical fiber by the second lens 14, the double-light envelope layer optical fiber guides the femtosecond laser into the endoscopic probe 2, and the endoscopic probe 2 scans a sample to be detected; two-photon fluorescence generated after a sample is excited by femtosecond laser is guided into a two-photon enveloping layer optical fiber through the endoscopic probe 2, the two-photon fluorescence emitted by the two-photon enveloping layer optical fiber is collimated by the second lens 14, then is transmitted through the plane dichroic reflector 13, is converged by the third lens 15, and then enters the detector 17 (specifically, the photomultiplier detector 17) to be converted into an electric signal.
In this embodiment, the tunable femtosecond laser 1 or the single-wavelength femtosecond laser 1 can be selected and used according to the operating wavelength range of the double-envelope fiber 16. For example, the tunable femtosecond laser 1 can select Chameleon Vision II (680-1080 nm, 140fs, 80MHz, 1W-2W) of Coherent in the United states; the single wavelength femtosecond laser 1 can be selected from FemtoFiber ultra920(920nm, <100fs, 80MHz, 1W-1.5W) of Toptica, Germany, and ALCOR920(920nm, 100fs, 80MHz, 1W-2W) of RPMC, Fascial. Since the working communication bands of the common dual-clad optical fiber 16 are 1310nm and 1550nm and the working wavelength ranges of 680nm to 1080nm are few, the dual-clad optical fiber 16 can be specially customized by an optical fiber manufacturer for a two-photon system using the femtosecond laser 1 (tunable 680nm to 1080nm and single wavelength of 920 nm).
The imaging system of the embodiment solves the problem of large size caused by adopting two paths of light to collect excitation light and signal light respectively in the prior technical scheme; the method can meet the requirement of in-vivo diagnosis of early tumor, and can be hung on the head of a freely moving mouse to obtain clear images of dynamic neural network activities of the mouse during free movement.
While the embodiments of the invention have been disclosed above, it is not limited to the applications listed in the description and the embodiments, which are fully applicable in all kinds of fields where the invention is suitable, and further modifications may readily be made by those skilled in the art, and the invention is therefore not limited to the specific details without departing from the general concept defined by the claims and the scope of equivalents.
Claims (10)
1. An endoscopic probe suitable for two-photon fluorescence imaging is characterized by comprising a double-envelope optical fiber, a first micro lens, an MEMS micro mirror and a second micro lens which are sequentially arranged along an incident light path;
the femtosecond laser emitted by the double-envelope optical fiber is collimated by the first micro lens and reflected by the MEMS micro mirror in sequence and then converged on a sample by the second micro lens; two-photon fluorescence generated by the sample is collected by the second micro lens and then is converged into the double-envelope optical fiber by the first micro lens after being reflected by the MEMS micro mirror;
the double-envelope optical fiber comprises a fiber core, a first envelope layer and a second envelope layer which are sequentially arranged from inside to outside, wherein the fiber core is used for transmitting femtosecond laser, and the first envelope layer is used for transmitting two-photon fluorescence generated by a sample.
2. The endoscopic probe adapted for two-photon fluorescence imaging according to claim 1, wherein the core and the first cladding layer have different refractive indices.
3. The endoscopic probe suitable for two-photon fluorescence imaging according to claim 2, wherein the double-envelope fiber is coaxial with the first microlens;
the plane of the MEMS micro-mirror and the optical axes of the first micro-lens and the second micro-lens form an included angle of 45 degrees, and the center of the MEMS micro-mirror is located at the intersection point of the optical axes of the first micro-lens and the second micro-lens.
4. The endoscopic probe suitable for two-photon fluorescence imaging according to claim 3, further comprising a probe mount comprising a housing and a fiber optic mount, a microlens mount and a micromirror mount disposed within the housing.
5. The endoscopic probe suitable for two-photon fluorescence imaging according to claim 4, wherein the optical fiber support has an optical fiber hole formed in the middle thereof for disposing the double-envelope optical fiber;
the bottom of the micro lens support is provided with an installation groove, the side surface of the micro lens support is provided with a first lens hole and a second lens hole which are perpendicular to each other and are communicated with the installation groove, the first micro lens is arranged in the first lens hole, and the second micro lens is arranged in the second lens hole;
the micro lens support is arranged on the side part of the optical fiber support, and the optical fiber hole is communicated with the first lens hole and shares a central axis;
the micro-mirror support is arranged in the mounting groove and is provided with a 45-degree inclined plane for arranging the MEMS micro-mirror.
6. The endoscopic probe suitable for two-photon fluorescence imaging according to claim 5, wherein the housing is cylindrical, and a window for passing the femtosecond laser and the two-photon fluorescence is formed on the housing above the second microlens.
7. The endoscopic probe suitable for two-photon fluorescence imaging according to claim 5, wherein the optical fiber holder further defines a tapered hole in the middle thereof, the tapered hole having a first end communicating with the optical fiber hole and a second end communicating with the first lens hole; the diameter of the first end of the tapered hole is equal to the diameter of the optical fiber hole, and the diameter of the second end of the tapered hole is equal to the diameter of the first lens hole.
8. An imaging system suitable for two-photon fluorescence imaging, comprising an endoscopic probe according to any of claims 1 to 7.
9. The imaging system suitable for two-photon fluorescence imaging of claim 8, further comprising a femtosecond laser, a first lens, a planar mirror, a planar dichroic mirror, a second lens, a third lens, and a detector.
10. The imaging system suitable for two-photon fluorescence imaging according to claim 9, wherein the femtosecond laser emitted by the femtosecond laser is collimated by the first lens, reflected by the plane reflector and the plane dichroic reflector in sequence, and then converged into the dual-optical envelope fiber by the second lens, the dual-optical envelope fiber guides the femtosecond laser into the endoscopic probe, and the endoscopic probe scans a sample to be measured; two-photon fluorescence generated after a sample is excited by femtosecond laser is guided into the two-photon enveloping layer optical fiber through the endoscopic probe, the two-photon fluorescence emitted by the two-photon enveloping layer optical fiber is collimated by the second lens, then transmits the plane dichroic reflector, and then is converged by the third lens and then enters the detector.
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Address after: 215163 Suzhou 88 high tech Zone, Jiangsu science and Technology City Patentee after: Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences Patentee after: Nanjing Guoke Precision Medical Technology Co.,Ltd. Address before: 215163 Suzhou 88 high tech Zone, Jiangsu science and Technology City Patentee before: Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences Patentee before: NANJING GUOKE MEDICAL TECHNOLOGY DEVELOPMENT Co.,Ltd. |
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