CN212184892U - Double-layer pail assembly and biological sample cryopreserving device - Google Patents

Abstract

The utility model provides a double-deck pail subassembly, including upper pail and lower floor's pail, upper pail includes upper staving and upper jib, and lower floor's pail includes lower floor's staving and lower floor's jib, and lower floor's jib includes long upper boom, long kink and long lower beam, and the projection of long kink passes the center of lower floor's staving to form a concave part with lower floor's staving, long lower beam, the minimum height that highly is greater than the upper staving of concave part, long lower beam are fixed in the lateral barrel wall of lower floor's staving. The utility model discloses simple structure, the integration degree is high, and is simple and convenient safe in utilization, can get the assigned position pail of putting accessible.

Description

Double-layer pail assembly and biological sample cryopreserving device

Technical Field

The utility model relates to a save field, the supplementary reproduction field among the medical science especially, in particular to a biological sample cryopreserving device that is used for double-deck pail subassembly of liquid nitrogen container and uses this subassembly.

Background

The biological sample of the present invention mainly refers to germ cells and tissues such as sperm, ovum, embryo, ovary tissue, and testis tissue, and is described herein mainly by taking semen and frozen sperm (hereinafter, referred to as "sperm") as an example. Sperm freezing refers to the application of certain technical means to preserve sperm at an ultra-low temperature of-196 ℃ and reduce the metabolic level for future use, preventing future fertility risks, also known as male fertility preservation, or reproductive insurance. The biological sample can recover biological activity after being taken out from the freezing device and recovered, and the method is a mature method for preserving fertility.

At present, there are two main forms of the common liquid nitrogen tank hoisting container for storing samples by freezing with liquid nitrogen. One is a multi-layer drawer type hanging box used for storing shorter freezing storage pipes in a cellular manner; the other is a single-layer cylindrical lifting barrel used for storing a plurality of longer freezing pipes.

The biological sample freezing and storing device is operated by placing the biological sample on a vitrification freezing carrying rod, placing the vitrification freezing carrying rod loaded with the biological sample into a freezing bracket, and then placing the freezing bracket into a lifting barrel; the tank opening of the liquid nitrogen tank is provided with a plurality of clamping grooves which are uniformly distributed along the circumferential direction of the tank opening and are radial; at last, the lifting bucket is hoisted in the liquid nitrogen tank, the lifting bucket comprises a bucket body and a hanging rod connected to the bucket body, a clamping hook is arranged at the upper end of the hanging rod, the bucket body extends into the liquid nitrogen tank, and the clamping hook can be clamped in a clamping groove to hoist the lifting bucket.

The current biological sample freezing device has defects in actual use: a plurality of buckets that the cooperation liquid nitrogen container used are the same bucket of specification in fact, that is to say, when a plurality of buckets were hoisted in the liquid nitrogen container through the cooperation of trip and draw-in groove respectively, the staving all was located same liquid level department, only can deposit the one deck bucket in the liquid nitrogen container, and the low-usage, the biological sample quantity that can deposit is very limited.

For a larger assisted reproduction center, if the average number of ova taking cycles exceeds 500 per month, the number of liquid nitrogen tanks required for freezing biological samples increases continuously per month, which causes large space occupation and can not meet large-scale sample freezing storage and development, and the liquid nitrogen tanks are expensive and high in use cost, which limits the development of an assisted reproduction center.

The pail is used more often in embryo carrier bar freezing, but in practice the carrier bar does not require a very high space, which is wasteful of space. Although there are multi-layer pail designs to increase the capacity of liquid nitrogen container, these designs mostly adopt coaxial rotary structure, if need to extract the sample in a certain pail, it needs to extract several layers of pails arranged on the same hanger bar at the same time, then take out the needed pail by rotation. This is inconvenient and delays time, and may also cause the risk of repeated freezing and thawing of the sample by passing the sample in and out of the liquid nitrogen for many times.

Chinese patent 205838302U discloses a novel multilayer liquid nitrogen tank short lifting bucket, referring to fig. 1, at least two grouped short lifting buckets and lifting rods with different lengths, which correspond to the short lifting buckets and are used for hanging the short lifting buckets on a liquid nitrogen tank opening, are arranged above and below each suspension area; one end of a suspender of each short lifting bucket is connected with the short lifting bucket, and the other end of the suspender forms a hook; a bent part is arranged on the suspension rod of each short lifting bucket, and the bent part is fixedly connected with a magnetic magnet; the hooks at the end parts of the hanging rods of a certain group are aligned, so that the magnets are close to each other, and the magnets are automatically combined together through magnetism, and all the short buckets of the certain group are sequentially vertically arranged in multiple layers from top to bottom to form a cylindrical shape. The combined magnets can be detached by applying force to the hooks to extract a layer of short pail. However, this kind of pail in groups shares a couple groove to adopt magnet to connect, when getting and putting arbitrary pail, two pails all need to operate together, especially when wanting to take out upper pail alone, need take out lower floor's pail earlier, waste time and energy, the interference is great between each other.

In addition, chinese patent CN208739994U discloses a biological sample cryopreservation device, referring to fig. 2, comprising a liquid nitrogen tank, a first lifting bucket and a second lifting bucket; the first lifting barrel comprises a first barrel body extending into the liquid nitrogen tank and a first lifting rod connected to the first barrel body, and a first clamping hook matched with the first lifting groove is arranged at the upper end of the first lifting rod; the second lifting barrel comprises a second barrel body extending into the liquid nitrogen tank and a second lifting rod connected to the second barrel body, and a second clamping hook matched with the second lifting groove is arranged at the upper end of the second lifting rod; the projections of the first barrel body and the second barrel body in the horizontal direction in the liquid nitrogen tank are not overlapped. However, the bucket structures of the upper layer and the lower layer of the freezing device are basically consistent, only have difference in height, and are basically consistent in the upper position and the lower position, and only are staggered by a very small amplitude. The possible movement track of the lifting barrels in the taking and placing process and the insufficient space in the liquid nitrogen tank are not considered, particularly when the lifting barrels on the upper layer are required to be taken out independently, the lifting barrels with the same structure are seriously collided and interfered with each other, an operator needs to take out the lifting barrels with special care, and the defect is particularly prominent when the small liquid nitrogen tank is used.

In addition, chinese patent CN108541703A discloses a biological sample cryopreservation device, referring to fig. 3, including a liquid nitrogen storage tank, and a cylindrical human embryo accommodating barrel located in the liquid nitrogen of the inner container and having an open top, the top surface of the annular heat-insulating inner cover is circumferentially and uniformly provided with eighteen hook accommodating grooves matched with the hooks, and each hook accommodating groove is hung with one human embryo accommodating barrel located in the liquid nitrogen of the inner container through the hook. The lifting bucket of the device also has only one structure, and when the lifting bucket at a specified position is taken and placed, the lifting buckets with the same structure are seriously collided and interfered with each other.

In view of this, in the clinical field of cryopreservation of biological samples, a double-layer pail assembly for a liquid nitrogen tank, which has a simple structure, is easy to operate, is safe to use, and can take and place a pail at a designated position without obstacles, is urgently needed.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the technical problem that a double-deck pail subassembly for liquid nitrogen container that simple structure, easy operation, safe in utilization, accessible get put assigned position pail is provided.

In order to solve the problem, the utility model provides a double-deck pail subassembly, including upper pail and lower floor's pail, the upper pail includes upper staving and upper jib, and lower floor's pail includes lower floor's staving and lower floor's jib, and lower floor's jib includes long upper boom, long kink and long lower beam, and the projection of long kink passes the center of lower floor's staving to form a concave part with lower floor's staving, long lower beam, the minimum height that highly is greater than the height of upper staving of concave part, long lower beam are fixed in the side bucket wall of lower floor's staving.

Further, the assembly is used for a liquid nitrogen tank.

Further, the upper suspender comprises a short upper rod, a short bending part and a short lower rod, and the projection of the short bending part does not enter the upper barrel body.

Furthermore, the upper end of the suspender is provided with a hook. The hook is used for being respectively matched with different clamping grooves at the opening part of the liquid nitrogen tank.

Further, the projection of the long bending part deviates by no more than an angle relative to the center of the lower-layer barrel body.

Further, when the upper layer pail and the lower layer pail are placed in the liquid nitrogen tank, the projections of the short bending part and the long bending part are evenly distributed around the center of the liquid nitrogen tank at intervals.

Further, the curved shape of the long bending part is matched with the curved shape of the wall of the liquid nitrogen tank.

Furthermore, the side wall of the pail is provided with leak holes, and the proportion of the total area occupied by the leak holes to the area of the side wall is less than 25%.

Further, the utility model also provides a biological sample cryopreserving device, including the liquid nitrogen container, its use is according to preceding an arbitrary double-deck pail subassembly. The clamping grooves are uniformly distributed at the opening part of the liquid nitrogen tank.

The utility model has the advantages that:

(1) a concave part is designed below the bending part of the lower layer pail, the concave parts between the adjacent lower layer pails are mutually communicated, a huge and communicated space is formed above the lower layer pail and used for accommodating the upper layer pail, and the limited inner space of the pail and the space above the lower layer pail are ingeniously utilized. Meanwhile, the position of the long lower rod is fixed at a position close to the wall of the liquid nitrogen tank, and the taking and the placing of the upper layer lifting barrel are not influenced.

(2) When upper pail and lower floor's pail were placed in the liquid nitrogen container simultaneously, the projection of all kinks was around the center evenly distributed of liquid nitrogen container has guaranteed when getting and put the pail, no matter upper strata or lower floor's pail all can not receive the independent of any interference to take out or put into. The upper pail can be put into from vertical direction earlier under the condition that lower pail has taken one's place, then radial movement pail gets into final placement position again, and in this process, the staving part of upper pail can get into final placement position through the recess of two lower layer pails unimpededly, and the jib part also can follow the space between two lower layer pails long upper boom and the long kink and move radially outwards unimpededly. The phenomenon of mutual collision and interference between the upper lifting bucket and the lower lifting bucket is greatly reduced, and any specific lifting bucket can be taken and placed.

(3) As long as upper and lower buckets with reasonable sizes are designed according to the size and the shape of the used liquid nitrogen tank, the utilization rate of the liquid nitrogen tank can be improved to the highest.

(4) The structure of the upper layer pail and the lower layer pail is very simple, firm and durable, the shape of the upper layer pail and the lower layer pail is basically consistent with that of the pail in use at present, the upper layer pail and the lower layer pail completely accord with the use habit of a freezing work operator, and the taking and placing operation does not need to be adjusted and adapted.

(5) Each hook corresponds to a clamping groove, the operation is simple, all technical effects can be simply realized as long as the upper lifting barrel and the lower lifting barrel are arranged at intervals, and the error probability is low.

(6) The upper layer of pail and the lower layer of pail, the projection in vertical direction is only partly overlapping, and upper and lower sight is unshielded, can observe the condition that holds the sample in the pail easily.

Drawings

FIG. 1 is a schematic view of a prior art pail construction;

FIG. 2 is a schematic diagram of a prior art cryopreservation apparatus;

FIG. 3 is a schematic diagram of a prior art liquid nitrogen storage tank;

FIG. 4 is a schematic view of the structure of the pail assembly of the present invention;

fig. 5 is a schematic structural diagram of an embodiment of the cryopreservation apparatus of the present invention;

fig. 6 is a schematic top view of an embodiment of the pail assembly according to the invention.

Detailed Description

The following will explain in detail the embodiments of the double-layer pail assembly and the biological sample cryopreservation device provided by the present invention with reference to the accompanying drawings.

Referring to fig. 4-6, the utility model discloses a double-deck pail subassembly 1, including upper pail 2 and lower floor pail 3, upper pail 2 includes upper staving 4 and upper jib 5, lower floor pail 3 includes lower floor staving 9 and lower floor jib 10, lower floor jib 10 includes long upper boom 11, long kink 12 and long lower beam 13, the projection of long kink 12 passes the center of lower floor staving 9 to with lower floor staving 9, long lower beam 13 forms a concave part 14, the minimum height that highly is greater than upper staving 4 of concave part 14, long lower beam 13 is fixed in the side bucket wall of lower floor staving 7. The assembly is for a liquid nitrogen tank 15.

The pail assembly 1 is an assembly adapted to be used with a liquid nitrogen container 15 which provides only a low temperature environment in which all biological samples are to be taken or placed into the liquid nitrogen container 15 by means of the pail assembly 1. The traditional operation process is that the lower layer pail is put in firstly, and then the upper layer pail is put in. And the utility model discloses then need not to get according to the order and put upper and lower layer pail, can get the pail of putting arbitrary fixed position alone, because in whole operation process, structural innovation design makes can not take place to interfere and collide between each part under the normal operating. For example, in the case of having placed two adjacent upper buckets 2, when wanting to place the lower bucket 3 below the middle of the two upper buckets 2, it is only necessary to align the lower bucket 3 with the slot corresponding to the tank opening, vertically place the lower bucket 3 down from the tank opening to the lower side of the upper bucket 2, then move the boom outward in the radial direction, and then snap the hook 16 into the slot 17 to complete the sample placement. In this case, the recess 14 is actually a shape of a letter of a Chinese character 'ao' which is open to the side of the center of the liquid nitrogen tank 15. A concave portion 14 is formed together with the upper surface of the lower tub 9 and the long underbar 13. In the space formed by the recess 14, the upper pail 2 can be placed and, in the correct operation, the long bend 12 and the long lower bar 13 of the lower pail 3 will move outwards along the gap between the upper booms 5 of the two upper pails 2, without parts of the upper pail 2 colliding with parts of the lower pail 3. The utility model is different from the prior art, and simply realizes the upper and lower layered storage without interference. In some cases where the height of the tank is sufficient, the height of the well 14 may be suitably increased to increase the well space for more flexible operation of the pail assembly 1. The projection of long kink 12 passes the center of lower floor staving 9, can keep all being the design of a symmetry between each pail, and unified specification and size are convenient to make, but need to explain a bit, the utility model discloses there are some clearances between each part, because after all not the pail of computer control gets puts the operation, operating personnel how much all can have some operating errors, can guarantee like this that the operation of the overwhelming majority is carried out in the error range that we can accept, and the probability that also the collision takes place is very low. Therefore, in this case, if the projection does not pass through the center of the barrel body, but slightly deflects by an angle, the design object of the present invention can be achieved.

The upper layer suspender 5 comprises a short upper rod 6, a short bending part 7 and a short lower rod 8, and the projection of the short bending part 7 does not enter the upper layer barrel body 4. Generally speaking, the upper layer hanger rod 5 is not a vertical rod which is straight up and straight down, because most of liquid nitrogen tanks have tank openings with areas obviously smaller than the area of the tank bottom, and an obvious outward expansion inclined plane is arranged below the tank opening, which is also suitable for the sealing requirement of the liquid nitrogen tank 15. Therefore, in order to make the most effective use of the space inside the tank, the actual position of the pail is set as close as possible to the outer wall of the tank, i.e. below the outwardly flared ramp, thus making use of the space outside the lower part of the tank, while leaving a considerable amount of public free space in the center of the tank for access to the pail.

The upper ends of the hanging rods 5 and 10 are provided with hooks 16. The hooks 16 are respectively matched with different clamping grooves 17 at the opening part of the liquid nitrogen tank 15. When the liquid nitrogen storage tank is used, the number of the clamping grooves needs to be designed, and the number has a direct relation with the volume of the liquid nitrogen tank 15, the diameter of the tank body and the size of the opening. If can only hold the one deck pail during the design, then use the utility model discloses a during the pail subassembly, then can hold two-layerly, it is corresponding, the quantity of draw-in groove 17 is in order to be many times more, just so can guarantee that every couple 16 can both correspond an independent draw-in groove 17, and this has also ensured when the pail is got and is put, can not bump and disturb at a jar mouth position.

When the upper and lower buckets 2 and 3 are placed in the liquid nitrogen tank 15, the projections of the upper hanger rods 5 (including the short bends 7) and the long bends 12 are evenly spaced around the center of the liquid nitrogen tank 15. Because the utility model discloses a draw-in groove 17 distribution density is fairly big, has gone out one time even in the application of certain model liquid nitrogen container, for example MVE XC 47-10 liquid nitrogen container, every liquid nitrogen container standard is furnished with ten stainless steel buckets, jar body oral area also corresponds and is provided with 10 draw-in grooves, and when we reformed transform it, will increase draw-in groove quantity one time and introduce the notion of upper and lower layer bucket, at this moment, just require upper and lower layer bucket to require the interval evenly to set up, otherwise adjacent bucket will be because diameter direction's interference and can't normally put into. In fact, the interval arrangement is very meaningful for placing and taking out, can utilize the space in the can to the maximum extent, and leaves enough space, so that operators can conveniently take and place the small barrels.

The curved shape of the bent portions 7, 12 matches the shape of the liquid nitrogen tank 15. When the pail is put into liquid nitrogen container 15, the pail position is very important, and this just as accomodate article in the case, and the space between the reduction object that can furthest of putting orderly article, the space is less means that the space is compacter between the article, and the space of wasting is also less. A conventional liquid nitrogen tank, as shown in FIGS. 2 and 3, has a smaller mouth portion for sealing and maintaining temperature, and a larger lower portion for holding more articles and liquid nitrogen.

The side walls of the lifting buckets 2 and 3 are provided with leakage holes 18, and the proportion of the total area occupied by the leakage holes 18 to the area of the side walls is less than 25%. The provision of a small aperture in the side wall at a certain height allows a portion of the liquid nitrogen to remain in the keg as it is removed, and allows the temperature of the sample to be maintained at a lower level, allowing sufficient operating time for the operator.

As shown in FIG. 5, the present invention also provides a biological sample cryopreservation device, comprising a liquid nitrogen tank 15, which uses any one of the double-layer pail assemblies 1 described above. The clamping grooves 17 are uniformly distributed at the opening part of the liquid nitrogen tank 15.

The utility model discloses still provide another embodiment, the projection of long kink 12 is skew for the center of lower floor's staving 9 and is not more than 45 degrees angles. In this case, although each of the lower buckets 3 is fixed at the predetermined position and tilted to one side in a top plan view, the angle of the deviation does not affect the radially outward movement of the lower hanger bar 10 throughout the operation, and the lower hanger bar 10 does not collide with the upper hanger bar 5 during the operation, but the angle is not too large depending on the distance between the adjacent buckets, and otherwise the buckets are excessively tilted to one side, which may cause new interference and discomfort in operation. According to the embodiment, the long bending part 12 has small influence on taking and placing of the biological sample of the lower-layer barrel body 9, the opening area of operability is large, shielding in the vertical direction is small, occupied middle public taking and placing space can be correspondingly increased in operation, and the mode has certain positive significance under the condition that the tank body is wide.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of improvements and decorations can be made without departing from the principle of the present invention, and these improvements and decorations should also be regarded as the protection scope of the present invention.

Claims (10)

1. The utility model provides a double-deck pail subassembly, includes upper pail (2) and lower floor pail (3), upper pail (2) include upper staving (4) and upper jib (5), and lower floor pail (3) include lower floor staving (9) and lower floor jib (10), its characterized in that, lower floor jib (10) are including long upper boom (11), long kink (12) and long lower beam (13), and the projection of long kink (12) passes the center of lower floor staving (9) to form a concave part (14) with lower floor staving (9), long lower beam (13), the minimum height that highly is greater than upper staving (4) of concave part (14), long lower beam (13) are fixed in the side barrel wall of lower floor staving (9).

2. The double-decker pail assembly according to claim 1, characterized in that the recess (14) is in the shape of a recess that is open towards the side of the centre when the assembly is used for a liquid nitrogen tank (15), and in the space formed by the recess (14) the upper pail (2) can be placed, and the long bent part (12) and the long lower bar (13) of the lower pail (3) can be moved outwards along the gap between the upper hanger bars (5) of the two upper pails (2).

3. The double-deck bucket lifting assembly according to claim 1, wherein the upper boom (5) further comprises a short upper rod (6), a short bend (7), and a short lower rod (8), the projection of the short bend (7) not entering the projection of the upper bucket body (4).

4. The double-layer pail assembly according to claim 1, characterized in that the upper ends of the upper layer hanger bar (5) and the lower layer hanger bar (10) are provided with hooks (16), and the hooks (16) are used for respectively matching with different clamping grooves (17) at the mouth of the liquid nitrogen tank (15).

5. The double-deck pail assembly of claim 1, wherein the projection of the long fold (12) deviates by no more than 45 degrees from the centre of the lower bucket body (9).

6. The double-decker pail assembly of claim 1, characterized in that the projections of the upper boom (5) and the long bend (12) are evenly spaced around the centre of the liquid nitrogen tank (15) when the upper pail (2) and lower pail (3) are placed in the liquid nitrogen tank (15).

7. The double-decker pail assembly of claim 1, characterized in that the curved shape of the long bend (12) matches the curved shape of the pail wall of the liquid nitrogen container (15).

8. Double-decker pail assembly according to claim 1, characterized in that the side walls of the pail (2, 3) are provided with weep holes (18), the total area occupied by the weep holes (18) accounting for less than 25% of the side wall area.

9. A biological specimen cryopreservation device comprising a liquid nitrogen tank (15) using a double-layer pail assembly according to any one of claims 1-8.

10. The biological specimen cryopreservation device of claim 9, wherein the clamping grooves (17) for matching with the pail assembly are evenly distributed at the mouth of the liquid nitrogen tank (15).

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