CN212134464U - Light-activated chemiluminescence micro-fluidic device and instant detection system - Google Patents
Light-activated chemiluminescence micro-fluidic device and instant detection system Download PDFInfo
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- CN212134464U CN212134464U CN201922462738.0U CN201922462738U CN212134464U CN 212134464 U CN212134464 U CN 212134464U CN 201922462738 U CN201922462738 U CN 201922462738U CN 212134464 U CN212134464 U CN 212134464U
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Abstract
The utility model relates to a detect technical field, relate to a light-activated chemiluminescence micro-fluidic device and instant detecting system for the technical problem that the detection device simple operation nature that exists is not enough and mechanism in the detecting system is in large quantity, its volume is difficult to further reduce among the solution prior art. The utility model discloses a light-activated chemiluminescence micro-fluidic device includes the sample cell, reagent unit and reaction unit, because sample cell and reagent unit are linked together with reaction unit controlled respectively, consequently can realize that the sample that awaits measuring in the sample cell and the acceptor granule and the donor granule in the reagent unit directly get into the reaction unit and carry out the homogeneous reaction when needs, thereby the transfer of material no longer relies on extra sampling mechanism and/or transfer mechanism in each unit, its convenience has obtained great improvement.
Description
Technical Field
The utility model relates to a detect technical field, relate to a light-activated chemiluminescence micro-fluidic device and instant detecting system especially.
Background
The Point-of-care Testing (POCT) technique has the characteristics of high sensitivity, high precision and wide range of the chemiluminescence immunoassay technique, and the POCT detection technique is rapid and portable, so that the POCT detection technique is widely concerned.
The detection device used in the current chemiluminescence instant test is generally a reagent card, for example, Chinese patent CN208568604U, which discloses a homogeneous chemiluminescence POCT detection device, wherein a plurality of holes are arranged on the reagent card of the device and are respectively used for bearing samples, reagents and the like, and because the holes are independent and not communicated with each other, if the reagents need to be added, a series of complex mechanisms such as a sampling mechanism, a transfer mechanism and the like need to be operated simultaneously to transfer substances in the holes, so that the operation mode is not convenient enough; further, due to the presence of these sampling mechanisms and transfer mechanisms, the number of complex mechanisms in the detection system is large, and the complex mechanisms further become bottlenecks that restrict the reduction of the volume of the detection system.
SUMMERY OF THE UTILITY MODEL
The utility model provides a light-activated chemiluminescence micro-fluidic device and instant detection system for solve the detection device simple operation nature that exists among the prior art not enough and detection system in mechanism quantity many, its volume be difficult to the technical problem who further reduces.
According to a first aspect of the present invention, there is provided a light-activated chemiluminescent microfluidic device comprising:
a sample unit for carrying a sample to be tested,
the reagent unit is used for bearing acceptor particles and donor particles required for homogeneous chemiluminescent reaction, wherein the donor particles can generate active oxygen after being excited, and the acceptor particles can react with the received active oxygen to generate chemiluminescence; and
the reaction unit is used for carrying out homogeneous reaction on a sample to be detected, the acceptor particles and the donor particles;
wherein the sample unit and the reagent unit are in controlled communication with the reaction unit, respectively.
In one embodiment, the sample unit and the reagent unit are respectively in communication with the reaction unit via microfluidic channels.
In one embodiment, the microfluidic channel is provided with a valve for controlling the on-off of the microfluidic channel.
In one embodiment, the valve is a pneumatically controlled microvalve.
In one embodiment, the sample unit comprises a pretreatment device and a sample pool which are connected in sequence, and the output end of the sample pool is connected with the reaction unit.
In one embodiment, the pretreatment device is provided with a sample outlet and a waste liquid outlet, the sample outlet is connected with the sample cell through the microfluidic channel, and the waste liquid outlet is connected with the sample waste liquid cell through the microfluidic channel.
In one embodiment, the pretreatment apparatus is provided with a separation apparatus, and the sample outlet is provided below the separation apparatus.
In one embodiment, the separation device is a blood filtration membrane.
In one embodiment, the reagent unit comprises a first reagent reservoir and a second reagent reservoir, which are connected to the reaction unit via the microfluidic channels, respectively.
In one embodiment, the reaction unit comprises a reaction cell, which is further connected to a common liquid bath.
According to the second aspect of the present invention, an instant detection system is provided, which comprises the above-mentioned photo-activated chemiluminescent microfluidic device, and further comprises a detection module for photo-activating and photo-detecting the substance in the reaction unit.
Compared with the prior art, the utility model has the advantages of: because the sample unit and the reagent unit are respectively and controllably communicated with the reaction unit, a sample to be detected in the sample unit and acceptor particles and donor particles in the reagent unit can directly enter the reaction unit for homogeneous reaction when needed, so that the transfer of substances in each unit does not depend on an additional sampling mechanism and/or a transfer mechanism, and the convenience is greatly improved; meanwhile, the sampling mechanisms and/or the transfer mechanisms are omitted, so that a complex mechanism for executing transfer work in the detection mechanism can be omitted, the number of mechanisms is reduced, the structure is simpler, the size of the detection system can be further reduced, and medical workers can conveniently carry the detection system to enter a diagnosis and treatment site.
Drawings
The present invention will be described in more detail hereinafter based on embodiments and with reference to the accompanying drawings.
Fig. 1 is a schematic plan view of an optical-chemical luminescent microfluidic device according to an embodiment of the present invention;
fig. 2 is a plan view of the pretreatment apparatus shown in fig. 1.
FIG. 3 is a sectional view of the pretreatment device shown in FIG. 2 at A-A.
Reference numerals:
1-sample unit;
11-a pretreatment device; 101-waste port; 102-sample outlet; 103-a separation device;
12-a sample cell; 13-sample waste reservoir;
2-a reagent unit; 21-a first reagent reservoir; 22-a second reagent reservoir;
3-a reaction unit; 31-a reaction tank;
4-microfluidic channels; 41-pneumatic control micro valve;
5-general liquid pool.
Detailed Description
The present invention will be further explained with reference to the accompanying drawings.
As shown in fig. 1, according to a first aspect of the present invention, there is provided a photo-activated chemiluminescent microfluidic device comprising a sample unit 1, a reagent unit 2 and a reaction unit 3. The sample unit 1 is used for bearing a sample to be detected, the reagent unit 2 is used for bearing acceptor particles and donor particles required by homogeneous phase chemiluminescence reaction, and the reaction unit 3 is used for enabling the sample to be detected, the acceptor particles and the donor particles to perform homogeneous phase reaction.
Wherein the donor particles are capable of generating reactive oxygen species upon excitation, and the acceptor particles are capable of reacting with the received reactive oxygen species to produce chemiluminescence.
The term "acceptor particle" as used herein refers to a particle that contains a compound that reacts with reactive oxygen species to produce a detectable signal. The donor particles are induced by energy or an active compound to activate and release reactive oxygen species in a high energy state that are captured by the acceptor particles in close proximity, thereby transferring energy to activate the acceptor particles. In some embodiments of the present invention, the acceptor particle comprises a luminescent composition and a carrier, and the luminescent composition is filled in the carrier and/or coated on the surface of the carrier. In the present invention, the "luminescent composition", i.e. a composition comprising a compound called a label, may undergo a chemical reaction in order to cause luminescence, for example by being converted into another compound formed in an electronically excited state. The excited state may be a singlet state or a triplet excited state. The excited state may relax to the ground state to emit light directly, or may return to the ground state itself by transferring excitation energy to an emission energy acceptor. In this process, the energy acceptor particle will be transitioned to an excited state to emit light. The acceptor particles may be present in the reagent unit 2 as a solid or as a liquid phase.
The term "donor particles" as used herein refers to particles containing a sensitizer capable of generating a reactive intermediate, such as a reactive oxygen species, that reacts with acceptor particles upon activation by energy or a reactive compound. The donor particles may be light activated (e.g., dyes and aromatic compounds) or chemically activated (e.g., enzymes, metal salts, etc.). In some embodiments of the invention, the donor particles are polymeric microspheres filled with a photosensitizer, which may be a photosensitizer known in the art, preferably a compound that is relatively light stable and does not react efficiently with singlet oxygen, non-limiting examples of which include compounds such as methylene blue, rose bengal, porphyrins, phthalocyanines, and chlorophylls disclosed in, for example, US5709994 (which patent is incorporated herein by reference in its entirety), and derivatives of these compounds having 1-50 atom substituents that are used to render these compounds more lipophilic or more hydrophilic, and/or as a linker group to a specific binding partner member. Examples of other photosensitizers known to those skilled in the art may also be used in the present invention, such as those described in US patent 6406913, which is incorporated herein by reference. The donor particles may be present in the reagent unit 2 in a solid or liquid phase.
Specifically, the sample unit 1 and the reagent unit 2 are controllably communicated with the reaction unit 3, respectively. As shown in FIG. 1, the sample unit 1 and the reaction unit 3 are in controlled communication, so that the sample to be tested in the sample unit 1 can be directly transferred to the reaction unit 3, and similarly, the acceptor particles and the donor particles in the reagent unit 2 can be directly transferred to the reaction unit 3, so that the sample to be tested, the acceptor particles and the donor particles can perform homogeneous reaction in the reaction unit 3, and the arrangement mode cancels a sampling and transferring mechanism in the existing device, so that the substance transfer between units can be directly performed without depending on an additional operating mechanism, and the detection convenience can be greatly improved.
Further, since the prior art uses a sampling and transferring mechanism to complete a series of operations such as sampling, transferring and sample adding, the sampling needle and the wells of the reagent card need to be aligned to each other in each operation to be operated accurately, which increases the complexity of the mechanism undoubtedly. And the utility model provides a detection device, because the material in sample unit 1 and the reagent unit 2 can only be shifted to reaction unit 3, consequently need not to consider the one-to-one relation between each unit to the complexity of mechanism has been reduced.
In one embodiment, as shown in fig. 1, a passage is provided between the sample unit 1 and the reaction unit 3 and between the reagent unit 2 and the reaction unit 3 to achieve mutual communication thereof. Specifically, the sample unit 1 and the reagent unit 2 are respectively communicated with the reaction unit 3 through microfluidic channels 4.
Furthermore, the microfluidic channel 4 is provided with a valve for controlling the on-off state thereof, so that the transfer of substances among the units can be conveniently controlled. Preferably, the valve is a pneumatically controlled microvalve 41. The pneumatic control micro valve 41 can be connected with a control unit, so that the micro-fluidic channel 4 can be opened or closed according to instructions, and the substances in each unit can be transferred to the designated direction according to the instructions.
In one embodiment, the microfluidic channel 4 is arranged obliquely to the reaction unit 3. Specifically, the microfluidic channel 4 is inclined toward the reaction unit 3, in other words, the upstream side of the microfluidic channel 4 is slightly higher than the downstream side thereof, so that the speed of transferring the substances in the sample unit 1 and the reagent unit 2 into the reaction unit 3 can be increased.
In a specific embodiment, the sample unit 1 comprises a pretreatment device 11 and a sample cell 12 connected in series, and the output end of the sample cell 12 is connected with the reaction unit 3. The pretreatment device 11 is used for a treatment before a test sample is detected, for example, analysis of serum or the like. Specifically, the pretreatment device 11 is a sample addition cell.
As shown in fig. 2, the pretreatment device 11 is provided with a sample outlet 102 and a waste liquid outlet 101, respectively, the sample outlet 102 is connected to the sample cell 12 through the microfluidic channel 4, and the waste liquid outlet 101 is connected to the sample waste liquid cell 13 through the microfluidic channel 4.
Further, the pretreatment device 11 is provided with a separation device 103, and the sample outlet 102 is provided below the separation device 103.
Preferably, as shown in fig. 3, the separation device 103 is a blood filtration membrane. In the existing device, before the detection, the original sample to be detected must be separated into the upper layer serum or plasma and the lower layer blood cells by the centrifugal action of the centrifugal device, and then the separated sample to be detected (serum or plasma) must be sucked by the sample conveying mechanism so as to perform the detection operation. And the utility model discloses in separator 103 who adopts for straining the blood membrane, it can fall into upper serum or plasma with original await measuring sample fast in the short time, and need not to set up centrifugal device and the supporting sample conveying mechanism who uses, consequently can further reduce and reduce detection device's part quantity and the complexity of mechanism to the realization is to the direct detection of whole blood.
After a sample to be tested is placed in the pretreatment device 11 and whole blood is separated by the separation device 103, serum or plasma flows into the sample cell 12 through the sample outlet 102 for detection, and waste liquid flows into the sample waste liquid cell 13 through the waste liquid outlet 101 for recovery.
The pneumatic micro valve 41 may be disposed in the microfluidic channel 4 between the pretreatment device 11 and the waste sample solution reservoir 13 and between the pretreatment device 11 and the sample reservoir 12, or the pneumatic micro valve 41 may not be disposed.
In a specific embodiment, the reagent unit 2 includes a first reagent cell 21 and a second reagent cell 22, and the first reagent cell 21 and the second reagent cell 22 are respectively connected to the reaction unit 3 through the microfluidic channel 4. As shown in fig. 1, the reagents in the first reagent reservoir 21 and the second reagent reservoir 22 respectively flow into the reaction unit 3 through the microfluidic channel 4 to perform a homogeneous reaction with the sample to be measured.
In a specific embodiment, the reaction unit 3 comprises a reaction tank 31, and the reaction tank 31 is further connected with the general liquid tank 5. As shown in fig. 1, the universal liquid in the universal liquid pool 5 can flow into the reaction unit 3 through the microfluidic channel 4 to react with the sample to be measured.
According to the second aspect of the present invention, an instant detection system is provided, which comprises the above-mentioned photo-activated chemiluminescent micro-fluidic device, and the instant detection system further comprises a detection module for adjusting the photo-activation and optical detection of the substance in the reaction unit 3. The detection module may adopt a structure in the prior art, and is not described herein again.
In summary, since the substance in the photo-activated chemiluminescent microfluidic device of the present invention can be directly transferred without any additional transfer mechanism, the sample adding mechanism and the transfer mechanism for transferring samples and reagents in the prior art are omitted, and therefore the number of mechanisms in the detection system and the complexity of the mechanisms can be reduced; on the other hand, the utility model discloses a separator among the light-activated chemiluminescence micro-fluidic device can directly separate original sample that awaits measuring to the centrifugal device who is used for separating original sample that awaits measuring among the prior art and the sample conveying mechanism who is used for shifting the centrifugal sample that awaits measuring etc. have been omitted, consequently, the quantity of mechanism and the complexity of reduction mechanism in the detecting system can further be reduced, thereby the bottleneck that detecting system's volume is difficult to reduce has been broken, make detecting system's structure simpler, it is more convenient to operate, it is more swift to detect.
While the invention has been described with reference to a preferred embodiment, various modifications may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention. In particular, the technical features mentioned in the embodiments can be combined in any way as long as there is no structural conflict. The present invention is not limited to the particular embodiments disclosed herein, but encompasses all technical solutions falling within the scope of the claims.
Claims (11)
1. A light-activated chemiluminescent microfluidic device, comprising:
a sample unit for carrying a sample to be tested,
a reagent unit for carrying acceptor particles and donor particles required for a homogeneous chemiluminescent reaction to occur; wherein the donor particles are capable of generating reactive oxygen species upon excitation, and the acceptor particles are capable of reacting with the received reactive oxygen species to generate chemiluminescence; and
the reaction unit is used for carrying out homogeneous reaction on a sample to be detected, the acceptor particles and the donor particles;
wherein the sample unit and the reagent unit are in controlled communication with a reaction unit, respectively.
2. The photoexcited chemiluminescent microfluidic device of claim 1 wherein the sample cell and the reagent cell are each in communication with the reaction cell via a microfluidic channel.
3. The optically activated chemiluminescent microfluidic device of claim 2 wherein the microfluidic channel is provided with a valve for controlling the on/off of the microfluidic channel.
4. The optically activated chemiluminescent microfluidic device of claim 3 wherein the valve is a pneumatically controlled microvalve.
5. The optically activated chemiluminescent microfluidic device according to any one of claims 2-4 wherein the sample cell comprises a pretreatment device and a sample cell connected in series, the output end of the sample cell being connected to the reaction cell.
6. The photoluminescence micro-fluidic device according to claim 5, wherein the pre-processing device is provided with a sample outlet and a waste liquid outlet, the sample outlet is connected with the sample cell through the micro-fluidic channel, and the waste liquid outlet is connected with the sample waste liquid cell through the micro-fluidic channel.
7. The photoluminescence microfluidic device according to claim 6, wherein a separation device is disposed in the pre-treatment device, and the sample outlet is disposed below the separation device.
8. The photostimulated chemiluminescent microfluidic device according to claim 7 wherein said separation device is a blood filter membrane.
9. The optically activated chemiluminescent microfluidic device according to any one of claims 2-4 wherein the reagent unit comprises a first reagent reservoir and a second reagent reservoir, the first reagent reservoir and the second reagent reservoir being connected to the reaction unit via the microfluidic channels, respectively.
10. The optically activated chemiluminescent microfluidic device of any one of claims 2-4 wherein the reaction cell comprises a reaction cell, the reaction cell further connected to a common liquid reservoir.
11. A point-of-care detection system comprising the photoluminescence microfluidic device of any one of claims 1-10, further comprising a detection module for photoexcitation and optical detection of the substances in the reaction cell.
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| CN201922462738.0U CN212134464U (en) | 2019-12-31 | 2019-12-31 | Light-activated chemiluminescence micro-fluidic device and instant detection system |
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| CN201922462738.0U CN212134464U (en) | 2019-12-31 | 2019-12-31 | Light-activated chemiluminescence micro-fluidic device and instant detection system |
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Address after: 200131 3rd and 5th floors, building 1, No.88 Cailun Road, Pudong New Area pilot Free Trade Zone, Shanghai Patentee after: Kemei Boyang diagnostic technology (Shanghai) Co.,Ltd. Patentee after: Kemei Diagnostic Technology Co., Ltd Address before: 201210 the third and fifth floors of Building 1, No. 88, Cailun Road, Pudong New Area, Shanghai Patentee before: BEYOND DIAGNOSTICS (SHANGHAI) Co.,Ltd. Patentee before: Kemei Diagnostic Technology Co., Ltd |
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