CN212065494U

CN212065494U - Cell cryopreserving box

Info

Publication number: CN212065494U
Application number: CN202020039600.XU
Authority: CN
Inventors: 陈廷涛
Original assignee: Nanchang University
Current assignee: Nanchang University
Priority date: 2020-01-09
Filing date: 2020-01-09
Publication date: 2020-12-04
Anticipated expiration: 2030-01-09

Abstract

The utility model discloses a cell cryopreserving box, including lid and box body, hinge swing joint box body is passed through to lid one end, and the last buckle of the lid other end and the lower buckle of box body cooperate, and lid upper end middle part fixed connection handle installs the backup pad in the box body, evenly be provided with 5 a 5 through-holes in the backup pad, be provided with two shell fragments in the through-hole, the shell fragment sets up along through-hole axis symmetry, places between the shell fragment and cryopreserved the pipe, and box body left side middle part is provided with the glass pipe, be provided with the scale mark on the glass pipe, glass pipe and box body internal portion communicate. This cell cryopreserved box utilizes the elasticity of shell fragment, places the cryopreserved pipe of different pipe diameters, through observing quartz glass pipe liquid level height, need not open the cryopreserved box, just can in time add isopropanol, guarantees the volume of isopropanol in the box body.

Description

Cell cryopreserving box

Technical Field

The utility model relates to a cell biology field, concretely relates to cell cryopreserving box.

Background

There are two common ways of freezing cells: the traditional freezing storage mode is that the cells are placed at 4 ℃ for 30-60 minutes, transferred to-20 ℃ for 30-60 minutes, then transferred to-80 ℃ for 16-18 hours, and finally placed in liquid nitrogen for long-term storage, and the traditional freezing storage mode has multiple steps, long time consumption, difficult control of the time of placing the cells at 4 ℃ and-20 ℃, non-uniform cooling rate, relatively large damage to the cells and low survival rate after recovery; the other type is a program cooling box sold in the market, cells added with freezing liquid are placed in the box, the cells are directly placed in liquid nitrogen for long-term storage at minus 80 ℃ overnight, good slow cooling rate can be provided, isopropanol is required to be added into the program cooling box, the isopropanol is volatile, in order to play a good program cooling effect, the amount of the isopropanol is required to be guaranteed and cannot be lower than a certain amount, the existing program cooling box generally adopts a low-temperature-resistant PE material, the transparency is poor, the liquid level height in the cooling box is not easy to observe, the liquid level height needs to be checked by opening a box cover, so that cold air in the freezing box is quickly lost to cause unfreezing of sample cells, the survival rate of the recovered cells is influenced, in addition, the existing program cooling box is single in function, only freezing tubes with a single structure can be placed, and the freezing tubes with.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a cell cryopreserving pipe to solve the problem that proposes in the above-mentioned background art. In order to achieve the above object, the utility model provides a following technical scheme:

a cell cryopreservation box comprises a box cover and a box body, wherein one end of the box cover is movably connected with the box body through a hinge, an upper buckle at the other end of the box cover is matched with a lower buckle of the box body, a handle is fixedly connected with the middle part of the upper end of the box cover, an annular sealing gasket is arranged at the lower end of the box cover, the annular sealing gasket is matched with the annular table at the upper part of the box body, a supporting plate is arranged in the box body, 5-5 through holes are uniformly arranged on the supporting plate, two elastic sheets are arranged in the through hole, the elastic sheets are symmetrically arranged along the axis of the through hole, the cryopreservation tube is arranged between the elastic sheets, the label paper is arranged beside the through hole, the middle part of the left side of the box body is provided with the glass tube, the glass tube is provided with scale marks, the glass tube is communicated with the interior of the box body, the bottom of the left side of the box body is provided with a liquid outlet tube, the liquid outlet pipe is provided with a liquid outlet valve, the right side of the box body is provided with a liquid inlet pipe, and the liquid inlet pipe is provided with a liquid inlet valve.

In this embodiment: the number of the upper buckles and the lower buckles is two.

In this embodiment: the number of the hinges is two.

In this embodiment: the label paper is sub-silver label paper.

In this embodiment: the material of the glass tube is quartz glass.

Compared with the prior art, the beneficial effects of the utility model are that: (1) according to the cell freezing storage box, one end of the box cover is movably connected with the box body through the hinge, the upper buckle at the other end of the box cover is matched with the lower buckle of the box body, and compared with the threaded connection in the prior art, the cell freezing storage box is easy to open after freezing; (2) label paper is arranged beside the through hole to record the information of the cryopreserved pipe, so that a user can conveniently and quickly search the information; (3) the spring plate is arranged in the through hole, so that on one hand, the spring plate plays a role in fixing and supporting, and on the other hand, the cryopreserved pipes with different pipe diameters can be placed by utilizing the elasticity of the spring plate; (4) set up the quartz glass pipe, quartz glass pipe and freeze and deposit the box inside intercommunication, through observing quartz glass pipe liquid level height, need not open and freeze and deposit the box, just can in time add the isopropyl alcohol, guarantee the volume of box body internal isopropyl alcohol.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a top view of the box body of the present invention;

reference numerals: handle 1, lid 2, annular sealing pad 3, hinge 4, go up buckle 5, lower buckle 6, glass pipe 7, feed liquor pipe 8, feed liquor valve 9, drain pipe 10, drain valve 11, box body 12, freeze and deposit pipe 13, backup pad 14, shell fragment 15, annular platform 16, through-hole 17, label paper 18.

Detailed Description

It is following to combine the attached drawing to be right the utility model discloses going on in detail, as shown in fig. 1 ~ 2, a cell cryopreserved box, including lid 2 and box body 12, 4 swing joint box bodies 12 of hinge are passed through to 2 one ends of lid, and the last buckle 5 of the 2 other ends of lid cooperatees with the lower buckle 6 of box body 12, compares prior art's threaded connection, opens easily after freezing, and lid 2 upper end middle part fixed connection handles 1 conveniently take the cell cryopreserved box.

In this embodiment: the lower end of the box cover 2 is provided with an annular sealing gasket 3, the annular sealing gasket 3 is matched with an annular table 16 on the upper part of the box body 12, the sealing performance of the cell cryopreservation box is guaranteed, and impurities are prevented from entering the cell cryopreservation box.

In this embodiment: install backup pad 14 in the box body 12, evenly be provided with 5 on the backup pad 14 by 5 through-holes 17, be provided with two shell fragments 15 in the through-hole 17, shell fragment 15 sets up along through-hole 17 axis symmetry, and shell fragment 15 plays the fixed stay effect on the one hand, and on the other hand can utilize the elasticity of shell fragment 15, places the cryopreserved pipe 13 of different pipe diameters, and the other label paper 18 that is provided with of through-hole 17, label paper 18 is used for the record cryopreserved pipe 13's information.

In this embodiment: 12 left sides of box body middle parts are provided with glass pipe 7, be provided with the scale mark on glass pipe 7,

glass pipe

7 and 12 inside being linked together of box body, through observing 7 liquid level heights of glass pipe, need not open the cryopreserved box, just can in time add the isopropyl alcohol, guarantee the volume of isopropyl alcohol in the box body, 12 left sides of box body bottom is provided with drain pipe 10, be provided with drain valve 11 on

drain pipe

10, 12 right sides of box body are provided with feed liquor pipe 8, be provided with feed liquor valve 9 on feed liquor pipe 8, drain pipe 10 and feed liquor pipe 8 are convenient to be changed the isopropyl alcohol to be convenient for control the height of the liquid in the box body 12.

In this embodiment: the number of the upper buckles 5 and the lower buckles 6 is two, and the number of the hinges 4 is two.

In this embodiment: the label paper 18 is a sub-silver label paper.

In this embodiment: the glass tube 7 is made of quartz glass, has small linear expansion coefficient and good chemical stability, and is suitable for the ultralow temperature environment.

The utility model discloses a theory of operation is: through shell fragment 15 in the extrusion through-hole 17, can place freezing of different pipe diameters and deposit pipe 13, shell fragment 15 has the effect of fixed stay to freezing and depositing pipe 13, through-hole 17's label paper can record freezing and deposit pipe 13 information, open feed liquor valve 9, pour into the isopropanol into to box body 12 through feed liquor pipe 8, glass pipe 7 and the inside of box body 12 are linked together, guarantee the injection volume of isopropanol through observing 7 liquid level heights of glass pipe, it is not enough to result in the volume when the isopropanol volatilizees, need not open and freeze and deposit the box, directly observe 7 liquid level heights of glass pipe, use more than 5 times when the isopropanol, open drain valve 11, through drain pipe 10 exhaust isopropanol.

The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.

Claims

1. A cell cryopreservation box is characterized in that: comprises a box cover (2) and a box body (12), one end of the box cover (2) is movably connected with the box body (12) through a hinge (4), an upper buckle (5) at the other end of the box cover (2) is matched with a lower buckle (6) of the box body (12), the middle part of the upper end of the box cover (2) is fixedly connected with a handle (1), an annular sealing gasket (3) is arranged at the lower end of the box cover (2), the annular sealing gasket (3) is matched with an annular table (16) at the upper part of the box body (12), a supporting plate (14) is installed in the box body (12), 5 x 5 through holes (17) are uniformly arranged on the supporting plate (14), two elastic sheets (15) are arranged in the through holes (17), the elastic sheets (15) are symmetrically arranged along the axis of the through holes (17), a freezing storage tube (13) is arranged between the elastic sheets (15), label paper (18) is arranged beside, be provided with the scale mark on glass pipe (7), glass pipe (7) and box body (12) are inside to be linked together, and box body (12) left side bottom is provided with drain pipe (10), be provided with drain valve (11) on drain pipe (10), box body (12) right side is provided with feed liquor pipe (8), be provided with feed liquor valve (9) on feed liquor pipe (8).

2. The cell cryopreservation kit of claim 1, wherein: the number of the upper buckles (5) and the lower buckles (6) is two.

3. The cell cryopreservation kit of claim 1, wherein: the number of the hinges (4) is two.

4. The cell cryopreservation kit of claim 1, wherein: the label paper (18) is a sub-silver label paper.

5. The cell cryopreservation kit of claim 1, wherein: the material of the glass tube (7) is quartz glass.