CN212051441U

CN212051441U - Biological incubator

Info

Publication number: CN212051441U
Application number: CN202020507957.6U
Authority: CN
Inventors: 曾小明; 王朝东
Original assignee: Shanghai Meiyue Biotech Development Co Ltd
Current assignee: Shanghai Meiyue Biotech Development Co Ltd
Priority date: 2020-04-09
Filing date: 2020-04-09
Publication date: 2020-12-01
Anticipated expiration: 2030-04-09

Abstract

The utility model provides a biological incubator, including the box, the box includes: a control unit forming a control command for adjusting the biological incubator; a temperature control unit receiving a control command to adjust an internal temperature of the biological incubator; the culture bin is communicated with the temperature control unit through an air channel; the culture vessels are placed in the culture bin and erected on the bottom plate of the culture bin, culture solution is contained in the culture vessels, and a magnetic element is placed in each culture vessel; the magnetic driving elements are fixedly arranged on the other side of the bottom plate opposite to the culture vessel, and each magnetic driving element corresponds to one magnetic element; each motor unit is electrically connected with a magnetic driving element; when the motor unit drives the magnetic driving element to rotate, the magnetic element is driven to move in the culture vessel so as to stir the culture solution. After the technical scheme is adopted, the biological incubator is small in size, easy to move and low in energy consumption, and the biological incubator can be accurately controlled by the controller to realize accurate culture of biological samples.

Description

Biological incubator

Technical Field

The utility model relates to a biological equipment field especially relates to a biological incubator.

Background

The biological incubator is used as a culture device of a laboratory biological sample, is widely used in many laboratories, is mainly used for a shake flask culture process parameter investigation experiment, a strain (cell) culture and a propagation small-sized experiment at the initial stage of biological sample fermentation, determines a shake flask fermentation process, and provides experimental data support for the process improvement of a fermentation pilot scale test, a pilot scale test and production. The existing biological incubator is large in volume and is usually fixed at a certain position, when the biological sample needs to be shake-cultured, the biological sample needs to be carried to the position of the biological incubator, and when the biological sample is not easy to carry and is not easy to see light, the traditional biological incubator is not suitable any more.

In addition, the traditional biological incubator adopts a three-phase high-power motor, the uniform oscillation rate and amplitude are adopted for each flask in the biological incubator, and when only part of the flasks need to oscillate, electric energy is wasted. And, the traditional biological incubator adopts the heat exchange of traditional compressor, and noise and energy consumption are too big.

Therefore, there is a need for a new type of biological incubator that can be made to have different oscillation rates for each flask by a simple operation, and that is more customized and controlled more accurately.

SUMMERY OF THE UTILITY MODEL

In order to overcome the technical defect, the utility model aims to provide a biological incubator, its is small, easily moves, and the energy consumption is low, and the accurate control of accessible controller to biological incubator realizes the accurate cultivation to the biological sample.

The utility model discloses a biological incubator, including the box, the box includes:

a control unit receiving a control operation to form a control instruction to adjust one or more of an internal temperature, an air flow rate, an air quality, an oxygen content of the biological incubator;

the temperature control unit is electrically connected with the control unit and receives a control instruction to adjust the internal temperature of the biological incubator;

the culture bin is communicated with the temperature control unit through an air channel;

at least one culture vessel which is arranged in the culture bin and is erected on the bottom plate of the culture bin, the culture solution is contained in the culture vessel, and a magnetic element is arranged in each culture vessel;

at least one magnetic driving element which is fixedly arranged on the other side of the bottom plate relative to the culture vessel, and each magnetic driving element corresponds to one magnetic element;

at least one motor unit, each motor unit is electrically connected with a magnetic driving element;

when the motor unit drives the magnetic driving element to rotate, the magnetic driving element drives the magnetic element to move in the culture vessel so as to stir the culture solution.

Preferably, the control unit includes:

the touch screen receives an interactive instruction applied to the biological incubator and forms control operation according to the interactive instruction;

and the controller is electrically connected with the touch screen, the temperature control unit and the motor unit, receives control operation and forms a corresponding control instruction, and stores one or more of the adjusted internal temperature, the air flow, the air quality, the oxygen content and the rotating speed of the motor unit in the box body.

Preferably, the temperature control unit includes:

one end of the circulating fan is communicated with the air channel;

the refrigeration chip is communicated with the other end, opposite to the air channel, of the circulating fan and is used for refrigerating the air in the air channel so as to reduce the temperature in the culture bin;

and the heating pipe is attached to the outer wall of the other end, opposite to the air channel, of the circulating fan, and is used for heating and refrigerating the air channel so as to raise the temperature in the culture bin.

Preferably, a first cooling fin is further arranged between the heating pipe and the outer wall to accelerate the cooling and/or heating speed of the cooling chip and/or the heating pipe to the air.

Preferably, the temperature control unit further comprises:

the second radiating fin is attached to the end face, opposite to the circulating fan, of the refrigeration chip;

the first fan is arranged on the end face, opposite to the refrigeration chip, of the second radiating fin and used for discharging heat generated by the refrigeration chip from the box body.

Preferably, the temperature control unit further comprises:

one end of the second fan is communicated with the external space of the box body, and the other end of the second fan is communicated with the air channel to guide external air into the box body;

the filter element is arranged between the heating pipe and the second fan and used for purifying the external air;

the side wall of the culture bin is provided with an air outlet which is communicated with the external space and discharges redundant air.

Preferably, the control instructions include: the expected temperature of the culture bin, the expected rotating speed and rotating time of any motor unit, the expected rotating speed of the second fan and the expected rotating speed of the first fan;

the culture cabin also comprises a sensor group which is used for detecting the temperature in the culture cabin and sending the temperature to the control unit for storage.

Preferably, the control unit further comprises an alarm unit;

when the rotation time is exhausted, the alarm unit sends out an alarm prompt.

Preferably, a first inner cavity and a second inner cavity which are arranged side by side are separated in the box body;

a first fan, a second radiating fin and a refrigerating chip are arranged in the first inner cavity;

the second inner cavity is internally provided with a second fan, a filter element, a heating pipe and a first radiating fin.

After the technical scheme is adopted, compared with the prior art, the method has the following beneficial effects:

1. the volume of the biological incubator of the utility model is only 1/4 of the traditional biological incubator, the volume is small, the weight is light, and the movement and the relocation are convenient;

2. the refrigeration chip has no time delay and buffer during refrigeration, the temperature control temperature difference of the biological sample is extremely small, and the constant temperature is accurate;

3. the rotating speed of the fan can be adjusted at will, so that the air flow is accurate;

4. the culture vessels are independently adjustable, so that the oscillation parameters of different culture vessels can be customized individually;

5. the strain process investigation period can be saved, multi-factor investigation is carried out simultaneously, and experimental data are more reliable;

6. under the condition of completely same temperature and other set conditions, the influence of different rotating speeds, different shearing forces and different dissolved oxygen (mature shake flask gauze and different shake flask loading) on the fermentation activity of the strain can be simultaneously inspected, and the inaccuracy of experimental data is reduced.

Drawings

FIG. 1 is a schematic view of a preferred embodiment of a biological incubator according to the present invention;

fig. 2 is a schematic structural diagram of a box body having a first inner cavity and a second inner cavity according to a preferred embodiment of the present invention.

Reference numerals:

1-control unit, 2: air outlet, 3: air passage, 4: culture vessel, 5: magnetic element, 6: magnetic drive element, 7: bottom plate, 8: a motor unit;

1-1: controller, 1-2: touch-sensitive screen, 1-3 second fin, 1-4: a first fan, 1-5: refrigeration chip, 1-6: a second fan, 1-7: a filter layer, 1-8: filter element, 1-9: heating pipe, 1-10: first fin, 1-11: and a circulating fan.

Detailed Description

The advantages of the present invention will be further explained with reference to the accompanying drawings and specific embodiments.

Reference will now be made in detail to the exemplary embodiments, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, like numbers in different drawings represent the same or similar elements unless otherwise indicated. The implementations described in the exemplary embodiments below are not intended to represent all implementations consistent with the present disclosure. Rather, they are merely examples of apparatus and methods consistent with certain aspects of the present disclosure, as detailed in the appended claims.

The terminology used in the present disclosure is for the purpose of describing particular embodiments only and is not intended to be limiting of the disclosure. As used in this disclosure and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any and all possible combinations of one or more of the associated listed items.

It is to be understood that although the terms first, second, third, etc. may be used herein to describe various information, such information should not be limited to these terms. These terms are only used to distinguish one type of information from another. For example, first information may also be referred to as second information, and similarly, second information may also be referred to as first information, without departing from the scope of the present disclosure. The word "if" as used herein may be interpreted as "at … …" or "when … …" or "in response to a determination", depending on the context.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience of description and for simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

In the description of the present invention, unless otherwise specified and limited, it is to be noted that the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, mechanically or electrically connected, or may be connected between two elements through an intermediate medium, or may be directly connected or indirectly connected, and specific meanings of the terms may be understood by those skilled in the art according to specific situations.

In the following description, suffixes such as "module", "part", or "unit" used to indicate elements are used only for the convenience of description of the present invention, and have no specific meaning in itself. Thus, "module" and "component" may be used in a mixture.

Referring to fig. 1, a schematic diagram of a preferred embodiment of a biological incubator according to the present invention includes:

-a box body

The box body forms the outline of the biological incubator, and in order to reduce the volume of the biological incubator and make the biological incubator easy to move, the length, the width and the height of the box body are set to be 500 x 550 x 700mm, so that the volume of the box body is only 1/4 of the traditional biological incubator.

Control unit 1

A control unit 1 is arranged in the box body, and when a user needs to adjust the parameters of the biological incubator, the control unit 1 or an operation part connected with the control unit 1 can be operated to apply control operation. When the control unit 1 receives a control operation, the control unit 1 will form control instructions to adjust one or more of the internal temperature, air flow, air quality, oxygen content, respectively, of the biological incubator, the control instructions being distributed by the control unit 1 to the devices controlling the above parameters.

-a temperature control unit

The temperature control unit is electrically connected with the control unit 1, receives a control instruction formed by the control unit 1, and adjusts the internal temperature of the biological incubator according to the requirement of the control instruction, so that different temperatures are adjusted according to the requirements of the oscillation environment of different biological samples.

-a culture chamber

The cultivation storehouse in the box forms a cavity for placing biological sample, cultivates the storehouse and communicates through an air passage 3 with the temperature control unit, then cultivates the temperature in the storehouse and is detected by the temperature control unit along with the conduction of air passage 3, otherwise the temperature change operation of temperature control unit also through the change to the air in the air passage 3, and then changes the temperature in cultivateing the storehouse. And the biological sample placed in the culture bin will be shaken or rocked in the culture bin to be sufficiently contacted with oxygen for culture.

Culture vessel 4

At least one culture vessel 4 is vertically arranged in the culture bin and is arranged on a bottom plate 7 of the culture bin, a culture solution is filled in the culture vessel 4, the culture solution comprises a biological sample, and the biological sample is oscillated or shaken in the culture vessel 4. In the preferred embodiment, a magnetic element 5, such as a magnetic ring, is further disposed in the culture vessel 4, and the magnetic element 5 is immersed in the culture solution or partially exposed from the culture solution.

A magnetic drive element 6

At least one magnetic driving element 6, such as a magnetic disc, is fixedly installed on the other side of the bottom plate 7 of the culture chamber, and the magnetic driving element 6 is opposite to or opposite to the culture vessel 4, i.e. the two sides of the bottom plate 7, the culture vessel 4 and the magnetic driving element 6 are respectively arranged, more preferably, the magnetic element 5 in the culture vessel 4 is opposite to the magnetic driving element 6, and the two are attracted by magnetism, so that when the magnetic driving element 6 is displaced, the magnetic element 5 is driven by magnetism to follow the magnetic driving element 6.

An electric motor unit 8

The motor units 8 are fixedly installed at the lower part of the bottom plate 7, i.e. the other side of the bottom plate 7 relative to the culture dish 4, and the number of the motor units 8 can be consistent with that of the magnetic driving elements 6, so that each magnetic driving element 6 is driven by one motor unit 8 to rotate or move.

When the motor unit 8 drives the magnetic driving element 6 to rotate, the magnetic element 5 is driven to move in the culture vessel 4 due to the magnetic force matching of the magnetic element 5 and the magnetic driving element 6, so that the culture solution in the culture vessel 4 is stirred, thallus in the culture solution can fully contact oxygen, and the change of the fermentation activity of the strain is observed.

It will be appreciated that the number of motor units 8 in the preferred embodiment may be one-to-many, one-to-one or one-to-many with the magnetic drive elements 6, i.e. fine adjustment of the direction of movement of the magnetic elements 5 may be made when each magnetic drive element 6 is provided with a motor unit 8 greater than one, whereas individual magnetic drive elements 6 are independently adjusted by a single motor unit 8 when each magnetic drive element 6 is provided with a motor unit 8 in one piece. Naturally, when only part of the culture vessels 4 need to be subjected to shaking culture, only the corresponding motor units 8 can be started, and the energy can be saved while independent and personalized adjustment can be made on different shaking requirements of different culture vessels 4.

In addition, through magnetic element 5 and the setting of magnetism drive element 6, abandoned former biological incubator completely and adopted the cultivation mode to the culture dish vibration so that the culture solution in it fully contacts oxygen to under the completely static application scene of culture dish, also can make the culture solution in it fully contact oxygen, the noise when having eliminated the culture dish vibration plays the key role to experimental environment's optimization.

Referring to fig. 2, in a preferred embodiment, the control unit 1 specifically includes:

touch screens 1-2

And a touch screen 1-2 capable of receiving interactive instructions applied to the biological incubator is arranged on the surface of the incubator body facing the user, and corresponding control operations such as temperature regulation, oscillation rate regulation, air volume regulation and the like are formed according to different interactive instructions applied to the touch screen 1-2 by the user. It can be understood that the touch screen 1-2 can be a liquid crystal touch screen 1-2, a touch screen 1-2 with physical key input, etc., thereby providing a concise and easy-to-understand biological incubator with quick operation for users.

Controller 1-1

The control unit 1 is internally provided with a controller 1-1 which is specially used for forming a control instruction, can be a single chip microcomputer and the like, and is respectively and electrically connected with the touch screen 1-2, the temperature control unit and the motor unit 8, after receiving the control operation, the controller 1-1 processes the control instruction to form a corresponding control instruction, and sends the control instruction to the temperature control unit and the motor unit 8, the internal temperature, the air flow, the air quality, the oxygen content, the rotating speed of the motor unit 8 and the like of the box body can be adjusted, and after each adjustment, the set parameters and the parameter abnormal change in the experimental process are stored, namely, for the output side, the control instruction formed by the controller 1-1 changes the internal parameters of the biological incubator, and for the input side, when the internal parameters of the biological incubator have unnecessary and irregular changes, the controller 1-1 detects and stores the internal parameters.

In a further preferred or alternative embodiment, the temperature control unit comprises:

circulating fans 1 to 11

The circulating fans 1-11 are arranged in the biological incubator, one end of each circulating fan is communicated with the air channel 3, and the circulating fans play roles in fresh air, purification and oxygen supply for the air in the air channel 3. Upon powering up the biological incubator, the circulation fans 1-11 may be configured to start operation to provide air to the incubation well.

Refrigeration chip 1-5

The refrigeration chip 1-5 is arranged at the other end of the circulating fan 1-11, namely the other end opposite to the air channel 3, when the circulating fan 1-11 starts to circulate fresh air in the culture bin, the air passing through the refrigeration chip 1-5 refrigerates the refrigeration chip 1-5, namely, after external air enters the biological incubator, the temperature of the external air is reduced by the refrigeration chip 1-5 and then is sent into the culture bin by the circulating fan 1-11, so that the temperature in the culture bin is reduced. Through the use of the refrigeration chips 1-5, the noise and the energy consumption of the compressor can be reduced, the control of the refrigeration chips 1-5 is more refined, and the control of the constant temperature in the culture bin is more stable.

Heating pipes 1 to 9

The circulating fan 1-11 is also provided with a heating pipe 1-9 on the outer wall side opposite to the other end of the air channel 3, and after the heating pipe 1-9 is electrified, the air in the air channel 3 is heated, so that the temperature in the culture bin is raised by utilizing the connectivity between the air channel 3 and the culture bin.

Through the design of the refrigerating chips 1-5 and the heating pipes 1-9, air in the air channel 3 can be respectively refrigerated or sent to the culture bin by the circulating fans 1-11, so that the temperature in the culture bin is ensured to be constant, and the temperature in the culture bin can be controlled to be 0-50 ℃, and more preferably set at any temperature between 10 ℃ and 40 ℃. In combination with the control of the control unit 1, its accuracy and precision is guaranteed.

Preferably or optionally, a first heat sink 1-10 is further disposed between the heating tube 1-9 and the outer wall, so as to increase the contact area when the cooling chip 1-5 or the heating tube 1-9 operates, thereby accelerating the cooling and/or heating speed of the cooling chip 1-5 and/or the heating tube 1-9 to the air.

Still further, the temperature control unit may further include:

second heat sink 1-3

The second cooling fin 1-3 is attached to the end face, opposite to the circulating fan 1-11, of the refrigeration chip 1-5, and because one end (the end facing the circulating fan 1-11) of the refrigeration chip 1-5 is a cold end, the end facing away from the circulating fan 1-11 is a hot end, and when the heat of the hot end is excessively deposited, the refrigeration capacity of the refrigeration chip is reduced. Therefore, the second radiating fins 1-3 are arranged to discharge the energy of the hot end to the outside of the box body.

First fan 1-4

The end face, namely the end part closer to the outside, of the second cooling fin 1-3 opposite to the refrigerating chip 1-5 is provided with a first fan 1-4, the first fan 1-4 can be a cooling fan, when the first fan is started, the heat conduction on the second cooling fin 1-3 is accelerated, and when the heat on the second cooling fin 1-3 is discharged out of the box body through the first fan 1-4, the second cooling fin 1-3 can absorb the heat at the hot end of the refrigerating new fin more, so that the refrigerating effect of the refrigerating chip 1-5 is accelerated. It is understood that the first fan 1-4 can be linked with the refrigeration chip 1-5, and when the refrigeration chip 1-5 is started, the first fan 1-4 is started along with the refrigeration chip.

Most preferably, the temperature control unit further comprises:

second fan 1-6

The second fan 1-6 is also arranged in the box body, one end of the second fan is communicated with the external space of the box body, for example, the second fan directly faces the outside of the box body, the other end of the second fan is communicated with the air channel 3, and after the second fan is started, the external air is guided into the box body, so that the oxygen increasing effect is achieved on the culture bin. In addition, the second fan 1-6 is connected with the control unit 1, the rotating speed of the second fan can be adjusted at will, so that the air flow in the culture bin can be accurately counted, and the second fan can be adaptively adjusted according to the oxygen demands of different thalli.

Filter elements 1 to 8

Between the second fan 1-6 and the heating pipe 1-9, there are filter elements 1-8, such as drawer type filter, filter layer 1-7 with PM2.5 and parts, etc. to purify the outside air, so as to ensure the quality of the air supplied to the culture chamber.

Meanwhile, the side wall of the culture bin is provided with an air outlet 2, the air outlet 2 is communicated with the culture bin and the external space, when the air supplied to the culture bin is excessive, the air is discharged outwards through the air outlet 2,

with the above configuration, the operation instructions that can be input on the touch panel 1-2 side include operation instructions that can form control instructions such as a desired temperature of the incubation chamber, a desired rotation speed and rotation time of any of the motor units 8, a desired rotation speed of the second fan 1-6, a desired rotation speed of the first fan 1-4, and the like. For a user, the accurate control can save a strain process investigation period, the investigation under multivariable and multifactor enables experimental data to be more reliable, namely, the influence of different rotating speeds, different shearing forces and different dissolved oxygen on strain fermentation activity is investigated under the condition that certain set conditions are completely the same, compared with the traditional biological incubator under the same temperature, one biological incubator can only set one rotating speed, different biological incubators are required to be used for comparison, or the investigation conditions of batch times are carried out, the investigation period is greatly reduced, and system errors and operation errors generated in different incubators and different batches of experiments are avoided.

Preferably or optionally, the control unit 1 further includes an alarm unit, and on the side of the control unit 1, the operating time of the motor unit 8 may be set, and when the rotation time of the motor unit 8 is exhausted, the alarm unit issues an alarm prompt, and the alarm prompt may be maintained for a period of time, such as 20 seconds, to notify the user that the current experiment has ended.

Because the first fan 1-4 and the second fan 1-6 have different functions, when the first fan 1-4 and the second fan 1-6 are arranged, a first inner cavity and a second inner cavity which are arranged side by side are formed by separating the inside of the box body, the first inner cavity is internally provided with the first fan 1-4, the second radiating fin 1-3 and the refrigerating chip 1-5, namely, the refrigeration control in the culture bin is finished in the first inner cavity, and the second inner cavity is internally provided with the second fan 1-6, the filtering element 1-8, the heating pipe 1-9 and the first radiating fin 1-10.

It should be noted that the embodiments of the present invention have better practicability and are not intended to limit the present invention in any way, and any person skilled in the art may change or modify the technical contents disclosed above to equivalent effective embodiments, but all the modifications or equivalent changes and modifications made to the above embodiments according to the technical spirit of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A biological incubator, comprising a housing, characterized in that the housing comprises:

a control unit receiving control operations to form control instructions to adjust one or more of the internal temperature, air flow, air quality, oxygen content of the biological incubator;

the temperature control unit is electrically connected with the control unit and receives the control instruction to adjust the internal temperature of the biological incubator;

at least one culture vessel which is arranged in the culture bin, is erected on the bottom plate of the culture bin, contains culture solution and is internally provided with a magnetic element;

2. The biological incubator as recited in claim 1,

the control unit includes:

the touch screen receives an interactive instruction applied to the biological incubator and forms a control operation according to the interactive instruction; and the controller is electrically connected with the touch screen, the temperature control unit and the motor unit, receives control operation, forms a corresponding control instruction, and stores one or more of the adjusted internal temperature, air flow, air quality, oxygen content and rotating speed of the motor unit in the box body.

3. The biological incubator as recited in claim 1,

the temperature control unit includes:

one end of the circulating fan is communicated with the air channel;

and the heating pipe is attached to the outer wall of the other end, opposite to the air channel, of the circulating fan and is used for heating the air channel so as to raise the temperature in the culture bin.

4. The biological incubator as recited in claim 3,

and a first radiating fin is arranged between the heating pipe and the outer wall to accelerate the cooling and/or heating speed of the cooling chip and/or the heating pipe to air.

5. The biological incubator as recited in claim 3,

the temperature control unit further includes:

the second cooling fin is attached to the end face, opposite to the circulating fan, of the refrigeration chip;

6. The biological incubator as recited in claim 5,

the temperature control unit further includes:

7. The biological incubator as recited in claim 6,

the control instructions include: the expected temperature of the culture bin, the expected rotating speed and rotating time of any motor unit, the expected rotating speed of the second fan and the expected rotating speed of the first fan;

the culture bin also comprises a sensor group which is used for detecting the temperature in the culture bin and sending the temperature to the control unit for storage.

8. The biological incubator as recited in claim 7,

the control unit also comprises an alarm unit;

and when the rotation time is exhausted, the alarm unit sends out an alarm prompt.

9. The biological incubator as recited in claim 6,

a first inner cavity and a second inner cavity which are arranged side by side are separated in the box body;

the first fan, the second cooling fin and the refrigeration chip are arranged in the first inner cavity;

the second inner cavity is internally provided with the second fan, the filter element, the heating pipe and the first radiating fin.