CN212025373U - Stem cell culture perfusion device - Google Patents
Stem cell culture perfusion device Download PDFInfo
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- CN212025373U CN212025373U CN202020325600.6U CN202020325600U CN212025373U CN 212025373 U CN212025373 U CN 212025373U CN 202020325600 U CN202020325600 U CN 202020325600U CN 212025373 U CN212025373 U CN 212025373U
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Abstract
The utility model relates to a stem cell culture perfusion device, including box, reducing mechanism, reaction unit, filter equipment, centrifugal device, washing device, culture apparatus, water storage device, stock solution device, first connecting pipe, second connecting pipe, third connecting pipe, fourth connecting pipe, fifth connecting pipe and sixth connecting pipe. The method has the advantages that the stem cell separation, purification and continuous perfusion are integrated, so that the material waste and material pollution of the treatment process are reduced; through the arrangement of the water storage device, the problem that the crushing device is not easy to clean is solved, and the problem that the humidity of the culture device cannot be controlled is also solved; and the stem cells after centrifugation are washed by using the washing device and centrifuged again, so that the purity of the stem cells is improved.
Description
Technical Field
The utility model relates to the field of biotechnology, especially, relate to a stem cell culture perfusion device.
Background
The extraction process of the placental stem cells comprises crushing, reaction, filtration, centrifugation and culture, and finally obtains a large amount of placental stem cells.
However, in the existing placental stem cell preparation equipment, the purity of the centrifuged placental stem cells is low due to impurities, which affects the subsequent culture. In addition, the humidity of the culture process is not easy to adjust, and the culture efficiency is affected.
Therefore, a stem cell culture perfusion device which can improve the purity of the placenta stem cells after centrifugation and can adjust the humidity in the culture process is needed.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a stem cell culture perfusion device aiming at the defects in the prior art.
In order to achieve the purpose, the utility model adopts the technical proposal that:
a stem cell culture perfusion device, comprising:
a box body;
the crushing device is arranged inside the box body and comprises a first feeding pipe and a first drainage pipe, the first end of the first feeding pipe is communicated with the crushing device, the second end of the first feeding pipe is positioned outside the box body, the first end of the first drainage pipe is communicated with the crushing device, and the second end of the first drainage pipe is positioned outside the box body;
the reaction device is arranged inside the box body and comprises a second feeding pipe, the first end of the second feeding pipe is communicated with the reaction device, and the second end of the second feeding pipe is positioned on the outer side of the box body;
the filtering device is arranged inside the box body;
the centrifugal device is arranged inside the box body and comprises a second drain pipe, the first end of the second drain pipe is communicated with the centrifugal device, and the second end of the second drain pipe is positioned outside the box body;
a washing device disposed inside the cabinet;
the culture device is arranged inside the box body and comprises a discharge pipe, a first end of the discharge pipe is communicated with the culture device, and a second end of the discharge pipe is positioned outside the box body;
the water storage device is arranged inside the box body and comprises a first water inlet pipe, a first water outlet pipe, a second water outlet pipe and a third water outlet pipe, wherein the first end of the first water inlet pipe is communicated with the water storage device, the second end of the first water inlet pipe is positioned on the outer side of the box body, the first end of the first water outlet pipe is communicated with the water storage device, the second end of the first water outlet pipe is communicated with the smashing device, the first end of the second water outlet pipe is communicated with the water storage device, the second end of the second water outlet pipe is communicated with the washing device, the first end of the third water outlet pipe is communicated with the water storage device, and the second end of the third water outlet pipe is communicated with the culturing device;
the liquid storage device is arranged inside the box body and comprises a liquid inlet pipe and a liquid outlet pipe, the first end of the liquid inlet pipe is communicated with the liquid storage device, the second end of the liquid inlet pipe is positioned on the outer side of the box body, the first end of the liquid outlet pipe is communicated with the liquid storage device, and the second end of the liquid outlet pipe is communicated with the culture device;
a first connecting pipe, a first end of which is communicated with the crushing device, and a second end of which is communicated with the reaction device;
a first end of the second connecting pipe is communicated with the reaction device, and a second end of the second connecting pipe is communicated with the filtering device;
a first end of the third connecting pipe is communicated with the filtering device, and a second end of the third connecting pipe is communicated with the centrifugal device;
a first end of the fourth connecting pipe is communicated with the centrifugal device, and a second end of the fourth connecting pipe is communicated with the washing device;
a fifth connecting pipe, a first end of which is communicated with the washing device and a second end of which is communicated with the centrifugal device;
and a first end of the sixth connecting pipe is communicated with the centrifugal device, and a second end of the sixth connecting pipe is communicated with the culture device.
Preferably, a first end of the first connecting pipe is connected with a lower end of the crushing device, and a second end of the first connecting pipe is connected with an upper end of the reaction device;
the first end of the second connecting pipe is connected with the lower end of the reaction device, and the second end of the second connecting pipe is connected with the upper end of the filtering device;
the first end of the third connecting pipe is connected with the lower end of the filtering device, and the second end of the third connecting pipe is connected with the upper end of the centrifugal device;
the first end of the fourth connecting pipe is connected with the lower end of the centrifugal device, and the second end of the fourth connecting pipe is connected with the upper end of the washing device;
a first end of the fifth connecting pipe is connected with the lower end of the washing device, and a second end of the fifth connecting pipe is connected with the upper end of the centrifugal device;
the first end of the sixth connecting pipe is connected with the lower end of the centrifugal device, and the second end of the sixth connecting pipe is connected with the upper end of the culture device.
Preferably, the first end of the first water outlet pipe is connected with the lower end of the water storage device, and the second end of the first water outlet pipe is connected with the upper end of the crushing device;
the first end of the second water outlet pipe is connected with the lower end of the water storage device, and the second end of the second water outlet pipe is connected with the upper end of the washing device;
the first end of the third water outlet pipe is connected with the lower end of the water storage device, and the second end of the third water outlet pipe is connected with the upper end of the culture device.
Preferably, the method further comprises the following steps:
the control device is arranged on the outer side of the box body and is respectively and electrically connected with the crushing device, the reaction device, the filtering device, the centrifugal device, the washing device, the culture device, the water storage device and the liquid storage device.
Preferably, the method further comprises the following steps:
the temperature control device is arranged inside the box body;
a temperature detection element disposed inside the case;
the control device is electrically connected with the temperature control device and the temperature detection element respectively.
Preferably, the method further comprises the following steps:
and the atomizing element is arranged at the second end of the third water outlet pipe and is electrically connected with the control device.
Preferably, the method further comprises the following steps:
the drying device comprises an air supply pipe, the first end of the air supply pipe is connected with the drying device, the second end of the air supply pipe is connected with the upper end of the crushing device, and the drying device is electrically connected with the control device.
Preferably, the method further comprises the following steps:
a first check valve disposed at a first end of the first connection pipe;
the second one-way valve is arranged at the first end of the second connecting pipe;
a third check valve disposed at a first end of the third connection pipe;
a fourth check valve disposed at a first end of the fourth connection pipe;
a fifth check valve disposed at a first end of the fifth connection pipe;
a sixth check valve disposed at a first end of the sixth connection pipe;
the seventh one-way valve is arranged at the first end of the first water outlet pipe;
the eighth one-way valve is arranged at the first end of the second water outlet pipe;
the ninth one-way valve is arranged at the first end of the third water outlet pipe;
the tenth one-way valve is arranged at the first end of the liquid outlet pipe;
an eleventh one-way valve disposed at a first end of the discharge tube.
The control device is electrically connected to the first check valve, the second check valve, the third check valve, the fourth check valve, the fifth check valve, the sixth check valve, the seventh check valve, the eighth check valve, the ninth check valve, the tenth check valve, and the eleventh check valve, respectively.
Preferably, the reaction device is arranged below the crushing device;
the filtering device is arranged below the reaction device;
the centrifugal device is arranged on the side of the filtering device;
the washing device is arranged on the side of the centrifugal device;
the culture device is arranged on the side of the washing device;
the water storage device is arranged above the crushing device;
the liquid storage device is arranged above the culture device.
Preferably, the method further comprises the following steps:
the first water pump is arranged on the third connecting pipe;
the second water pump is arranged on the fourth connecting pipe;
the third water pump is arranged on the fifth connecting pipe;
and the fourth water pump is arranged on the sixth connecting pipe.
The utility model adopts the above technical scheme, compare with prior art, have following technological effect:
the stem cell culture perfusion device integrates stem cell separation, purification and continuous perfusion, and reduces material waste and material pollution of the treatment process; through the arrangement of the water storage device, the problem that the crushing device is not easy to clean is solved, and the problem that the humidity of the culture device cannot be controlled is also solved; and the stem cells after centrifugation are washed by using the washing device and centrifuged again, so that the purity of the stem cells is improved.
Drawings
Fig. 1 is a cross-sectional view of an exemplary embodiment of the present invention.
Wherein the reference numerals are: the device comprises a box body 1, a crushing device 2, a reaction device 3, a filtering device 4, a centrifugal device 5, a washing device 6, a culture device 7, a water storage device 8, a liquid storage device 9, a first feeding pipe 10, a first water discharge pipe 11, a second feeding pipe 12, a second water discharge pipe 13, a discharge pipe 14, a first water inlet pipe 15, a first water discharge pipe 16, a second water discharge pipe 17, a third water discharge pipe 18, a liquid inlet pipe 19, a liquid discharge pipe 20, a first connecting pipe 21, a second connecting pipe 22, a third connecting pipe 23, a fourth connecting pipe 24, a fifth connecting pipe 25, a sixth connecting pipe 26, a control device 27, a temperature control device 28, a temperature detection element 29, an atomization element 30, a drying device 31, a first check valve 32, a second check valve 33, a third check valve 34, a fourth check valve 35, a fifth check valve 36, a sixth check valve 37, a seventh check valve 38, an eighth check valve 39, a ninth check valve 40, a third check valve 33, A tenth check valve 41, an eleventh check valve 42, a first water pump 43, a second water pump 44, a third water pump 45, a fourth water pump 46, and a blower pipe 47.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that, in the present invention, the embodiments and features of the embodiments may be combined with each other without conflict.
The present invention will be further described with reference to the accompanying drawings and specific embodiments, but the present invention is not limited thereto.
An exemplary embodiment of the present invention, as shown in fig. 1, a stem cell culture perfusion apparatus includes: the box body 1, the crushing device 2, the reaction device 3, the filtering device 4, the centrifuging device 5, the washing device 6, the culture device 7, the water storage device 8, the liquid storage device 9, the control device 27, the temperature control device 28, the temperature detection element 29 and the drying device 31 are arranged in the box body 1, the crushing device 2, the reaction device 3, the filtering device 4, the centrifuging device 5, the washing device 6, the culture device 7, the water storage device 8, the liquid storage device 9, the temperature control device 28, the temperature detection element 29 and the drying device 31 are arranged in the box body 1, and the control device 27 is arranged outside the box body 1.
The crushing device 2 comprises a first feeding pipe 10 and a first water discharge pipe 11, wherein the first end of the first feeding pipe 10 is connected with the upper part of the crushing device 2, the second end of the first feeding pipe 10 is positioned on the outer side of the box body 1, the first end of the first water discharge pipe 11 is connected with the lower part of the crushing device 2, and the second end of the first water discharge pipe 11 is positioned on the outer side of the box body 1.
Further, the first drainage pipe 11 is inclined to ensure that the sewage in the crushing device 2 can be drained through the first drainage pipe 11.
The reaction unit 3 is located below the crushing unit 2 and includes a second feeding pipe 12, and a first end of the second feeding pipe 12 is connected to an upper portion of the reaction unit 3. A first connecting pipe 21 is provided between the crushing device 2 and the reaction device 3, a first end of the first connecting pipe 21 is connected to a lower end of the crushing device 2, and a second end of the first connecting pipe 21 is connected to an upper end of the reaction device 3.
Further, a first check valve 32 is provided at the first end of the first connecting pipe 21 for controlling the material in the crushing apparatus 2 to enter the reaction apparatus 3.
The filter device 4 is located below the reaction device 3. A second connection pipe 22 is provided between the reaction device 3 and the filtering device 4, a first end of the second connection pipe 22 is connected to a lower end of the reaction device 3, and a second end of the second connection pipe 22 is connected to an upper end of the filtering device 4.
Further, a second check valve 33 is disposed at a first end of the second connection pipe 22 for controlling the material in the reaction device 3 to enter the filtering device 4.
The centrifugal device 5 is located at the side of the filtering device 4 and includes a second drain pipe 13, a first end of the second drain pipe 13 is connected with a lower end of the centrifugal device 5, and a second end of the second drain pipe 13 is located at the outer side of the case 1.
Further, the second drain pipe 13 is provided obliquely, ensuring that the sewage in the centrifugal device 5 can be discharged through the second drain pipe 13.
A third connection pipe 23 is provided between the filtering means 4 and the centrifuging means 5, a first end of the third connection pipe 23 is connected to the lower end of the filtering means 4, and a second end of the third connection pipe 23 is connected to the upper end of the centrifuging means 5.
Further, a third check valve 34 is provided at the first end of the third connection pipe 23 for controlling the material in the filtering means 4 to enter the centrifugal means 5.
Further, a first water pump 43 is provided in the third connecting pipe 23 downstream of the third check valve 34 for pumping the material in the filtering means 4 into the centrifugal means 5.
The washing device 6 is located at the side of the centrifugal device 5. A fourth connecting pipe 24 and a fifth connecting pipe 25 are provided between the centrifugal device 5 and the washing device 6, a first end of the fourth connecting pipe 24 is connected with a lower end of the centrifugal device 5, a second end of the fourth connecting pipe 24 is connected with an upper end of the washing device 6, a first end of the fifth connecting pipe 25 is connected with a lower end of the washing device 6, and a second end of the fifth connecting pipe 25 is connected with an upper end of the centrifugal device 5.
Further, a fourth check valve 35 is disposed at the first end of the fourth connection pipe 24 for controlling the material in the centrifugal device 5 to enter the washing device 6.
Further, a second water pump 44 is provided downstream of the fourth check valve 35 of the fourth connection pipe 24 for pumping the material in the centrifugal device 5 into the washing device 6.
Further, a fifth check valve 36 is provided at a first end of the fifth connection pipe 25 for controlling the material in the washing device 6 to enter the centrifugal device 5.
Further, a third water pump 45 is provided downstream of the fifth check valve 36 of the fifth connection pipe 25 for pumping the material in the washing device 6 into the centrifugal device 5.
The culture device 7 is arranged at the side of the washing device 6 and comprises a discharge pipe 14, a first end of the discharge pipe 14 is connected with the lower end of the culture device 7, and a second end of the discharge pipe 14 is positioned at the outer side of the tank body 1.
Furthermore, an eleventh non-return valve 42 is provided at the first end of the tapping pipe 14 for controlling the outward feeding of the material of the culture device 7.
A sixth connecting pipe 26 is provided between the centrifuge 5 and the culture apparatus 7, and a first end of the sixth connecting pipe 26 is connected to the lower end of the centrifuge 5 and a second end of the sixth connecting pipe 26 is connected to the upper end of the culture apparatus 7.
Further, a sixth check valve 37 is provided at the first end of the sixth connection tube 26 for controlling the material in the centrifugal device 5 to enter the culture device 7.
Further, a fourth water pump 46 is provided downstream of the sixth check valve 37 of the sixth connection pipe 26 for pumping the material in the centrifuge 5 into the culture apparatus 7.
The water storage device 8 is arranged above the smashing device 2 and comprises a first water inlet pipe 15, a first water outlet pipe 16, a second water outlet pipe 17 and a third water outlet pipe 18, wherein the first end of the first water inlet pipe 15 is connected with the upper end of the water storage device 8, the second end of the first water inlet pipe 15 is positioned on the outer side of the box body 1, the first end of the first water outlet pipe 16 is connected with the lower end of the water storage device 8, the second end of the first water outlet pipe 16 is connected with the upper end of the smashing device 2, the first end of the second water outlet pipe 17 is connected with the lower end of the water storage device 8, the second end of the second water outlet pipe 17 is connected with the upper end of the washing device 6, the first end of the third water outlet pipe 18 is connected with the lower end of the water storage device 8.
Further, a seventh one-way valve 38 is disposed at the first end of the first water outlet pipe 16, and is used for controlling water in the water storage device 8 to enter the crushing device 2, so as to clean the crushing device 2.
Furthermore, an eighth one-way valve 39 is disposed at the first end of the second water outlet pipe 17, and is used for controlling water in the water storage device 8 to enter the washing device 6, so as to clean the centrifuged stem cells.
Further, a ninth one-way valve 40 is disposed at the first end of the third water outlet pipe 18, and is used for controlling water in the water storage device 8 to enter the culture device 7, so as to adjust the humidity in the culture device 7.
Further, an atomizing element 30 is disposed at the second end of the third water outlet pipe 18 and inside the culture device 7 for atomizing the water delivered by the third water outlet pipe 18 to uniformly humidify the culture device 7.
The liquid storage device 9 is arranged on the lateral portion of the water storage device 8 and located above the culture device 7, and comprises a liquid inlet pipe 19 and a liquid outlet pipe 20, the first end of the liquid inlet pipe 19 is connected with the upper end of the liquid storage device 9, the second end of the liquid inlet pipe 19 is located on the outer side of the box body 1, the first end of the liquid outlet pipe 20 is connected with the lower end of the liquid storage device 9, and the second end of the liquid outlet pipe 20 is connected with the upper end of the culture device 7.
Further, a tenth check valve 41 is disposed at the first end of the liquid outlet tube 20 for controlling the culture liquid in the liquid storage device 9 to enter the culture device 7.
A temperature control device 28 is provided above the cabinet 1 for adjusting the temperature inside the cabinet 1, such as heating and cooling operations.
A temperature detecting element 29 is provided at a side of the temperature control device 28 for detecting the temperature inside the cabinet 1 for the operator to make a temperature adjustment reference.
The drying device 31 is arranged above the crushing device 2 and comprises an air supply pipe 47, a first end of the air supply pipe 47 is connected with the lower end of the drying device 31, and a second end of the air supply pipe 47 is connected with the upper end of the crushing device 2.
The control device 27 is disposed outside the tank 1, and is electrically connected to the pulverizing device 2, the reaction device 3, the filtration device 4, the centrifugation device 5, the washing device 6, the culture device 7, the water storage device 8, the liquid storage device 9, the temperature control device 28, the temperature detection element 29, the atomizing element 30, the drying device 31, the first check valve 32, the second check valve 33, the third check valve 34, the fourth check valve 35, the fifth check valve 36, the sixth check valve 37, the seventh check valve 38, the eighth check valve 39, the ninth check valve 40, the tenth check valve 41, the eleventh check valve 42, the first water pump 43, the second water pump 44, the third water pump 45, and the fourth water pump 46, respectively, so as to control the above devices, valves, or pumps.
The utility model discloses a use method as follows: firstly, the placenta is conveyed into a crushing device 2 through a first feeding pipe 10, and the crushing device 2 crushes the placenta; after the crushing is finished, the control device 27 starts the first one-way valve 32, and the placenta in the crushing device 2 is conveyed into the reaction device 3 through the first connecting pipe 21; conveying the reaction enzyme into the reaction device 3 through the second feeding pipe 12, so that the reaction enzyme reacts with the placenta; after the reaction, the control device 27 starts the second one-way valve 33, and the reaction liquid in the reaction device 3 is conveyed into the filtering device 4 through the second connecting pipe 22, and is filtered to obtain suspended matters; the control device 27 starts the third one-way valve 34 and the first water pump 43, suspended matters in the filtering device 4 are conveyed into the centrifugal device 5 through the third connecting pipe 23, centrifugation is carried out, and supernatant is discharged from the second water discharge pipe 13; the control device 27 starts the fourth one-way valve 35 and the second water pump 44, and the centrifuged solid is conveyed into the washing device 6 through the fourth connecting pipe 24; the control device 27 starts the eighth one-way valve 39, and the water in the water storage device 8 is conveyed into the washing device 6 through the second water outlet pipe 17 to wash the solid; the control device 27 starts the fifth one-way valve 36 and the third water pump 45, the suspension in the washing device 6 is conveyed into the centrifugal device 5, the suspension is centrifuged, and the supernatant is discharged from the second water discharge pipe 13; the control device 27 starts the sixth one-way valve 37 and the fourth water pump 44, and the solid in the centrifugal device 5 is conveyed into the culture device 7; the control device 27 starts the tenth one-way valve 41, and the culture solution in the liquid storage device 9 is conveyed into the culture device 7 through the liquid outlet pipe 20; according to the humidity condition in the culture device 7, the control device 27 starts the ninth one-way valve 40 and the atomizing element 30, water in the water storage device 8 is conveyed into the culture device 7 through the third water outlet pipe 18, and is atomized through the atomizing element 30, so that the humidity in the culture device 7 is adjusted; after the culture is finished, the control device 27 starts the eleventh check valve 42, and the stem cells in the culture device 7 are conveyed to the outside through the discharge pipe 14; when the temperature detecting element 29 detects that the temperature in the box body 1 is too low, the control device 27 starts the temperature control device 28 to heat the box body 1; when the temperature detecting element 29 detects that the temperature in the box body 1 is too high, the control device 27 starts the temperature control device 28 to perform the refrigeration operation on the box body 1;
when the crushing device 2 needs to be cleaned, the control device 27 starts the seventh one-way valve 37, water in the water storage device 8 is conveyed into the crushing device 2 through the first water outlet pipe 16, and the crushing device 2 is cleaned; the control device 27 activates the drying device 31, and the drying device 31 blows air into the pulverizing device 2 through the air blowing pipe 47 to dry the cleaned pulverizing device 2.
The above description is only an example of the preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and those skilled in the art should be able to realize the equivalent alternatives and obvious variations of the present invention.
Claims (10)
1. A stem cell culture perfusion device, comprising:
a box body;
the crushing device is arranged inside the box body and comprises a first feeding pipe and a first drainage pipe, the first end of the first feeding pipe is communicated with the crushing device, the second end of the first feeding pipe is positioned outside the box body, the first end of the first drainage pipe is communicated with the crushing device, and the second end of the first drainage pipe is positioned outside the box body;
the reaction device is arranged inside the box body and comprises a second feeding pipe, the first end of the second feeding pipe is communicated with the reaction device, and the second end of the second feeding pipe is positioned on the outer side of the box body;
the filtering device is arranged inside the box body;
the centrifugal device is arranged inside the box body and comprises a second drain pipe, the first end of the second drain pipe is communicated with the centrifugal device, and the second end of the second drain pipe is positioned outside the box body;
a washing device disposed inside the cabinet;
the culture device is arranged inside the box body and comprises a discharge pipe, a first end of the discharge pipe is communicated with the culture device, and a second end of the discharge pipe is positioned outside the box body;
the water storage device is arranged inside the box body and comprises a first water inlet pipe, a first water outlet pipe, a second water outlet pipe and a third water outlet pipe, wherein the first end of the first water inlet pipe is communicated with the water storage device, the second end of the first water inlet pipe is positioned on the outer side of the box body, the first end of the first water outlet pipe is communicated with the water storage device, the second end of the first water outlet pipe is communicated with the smashing device, the first end of the second water outlet pipe is communicated with the water storage device, the second end of the second water outlet pipe is communicated with the washing device, the first end of the third water outlet pipe is communicated with the water storage device, and the second end of the third water outlet pipe is communicated with the culturing device;
the liquid storage device is arranged inside the box body and comprises a liquid inlet pipe and a liquid outlet pipe, the first end of the liquid inlet pipe is communicated with the liquid storage device, the second end of the liquid inlet pipe is positioned on the outer side of the box body, the first end of the liquid outlet pipe is communicated with the liquid storage device, and the second end of the liquid outlet pipe is communicated with the culture device;
a first connecting pipe, a first end of which is communicated with the crushing device, and a second end of which is communicated with the reaction device;
a first end of the second connecting pipe is communicated with the reaction device, and a second end of the second connecting pipe is communicated with the filtering device;
a first end of the third connecting pipe is communicated with the filtering device, and a second end of the third connecting pipe is communicated with the centrifugal device;
a first end of the fourth connecting pipe is communicated with the centrifugal device, and a second end of the fourth connecting pipe is communicated with the washing device;
a fifth connecting pipe, a first end of which is communicated with the washing device and a second end of which is communicated with the centrifugal device;
and a first end of the sixth connecting pipe is communicated with the centrifugal device, and a second end of the sixth connecting pipe is communicated with the culture device.
2. The stem cell culture perfusion apparatus of claim 1, wherein a first end of the first connection tube is connected to a lower end of the comminution device and a second end of the first connection tube is connected to an upper end of the reaction device;
the first end of the second connecting pipe is connected with the lower end of the reaction device, and the second end of the second connecting pipe is connected with the upper end of the filtering device;
the first end of the third connecting pipe is connected with the lower end of the filtering device, and the second end of the third connecting pipe is connected with the upper end of the centrifugal device;
the first end of the fourth connecting pipe is connected with the lower end of the centrifugal device, and the second end of the fourth connecting pipe is connected with the upper end of the washing device;
a first end of the fifth connecting pipe is connected with the lower end of the washing device, and a second end of the fifth connecting pipe is connected with the upper end of the centrifugal device;
the first end of the sixth connecting pipe is connected with the lower end of the centrifugal device, and the second end of the sixth connecting pipe is connected with the upper end of the culture device.
3. The stem cell culture and perfusion device according to claim 1, wherein a first end of the first water outlet pipe is connected with a lower end of the water storage device, and a second end of the first water outlet pipe is connected with an upper end of the crushing device;
the first end of the second water outlet pipe is connected with the lower end of the water storage device, and the second end of the second water outlet pipe is connected with the upper end of the washing device;
the first end of the third water outlet pipe is connected with the lower end of the water storage device, and the second end of the third water outlet pipe is connected with the upper end of the culture device.
4. The stem cell culture perfusion apparatus of claim 1, further comprising:
the control device is arranged on the outer side of the box body and is respectively and electrically connected with the crushing device, the reaction device, the filtering device, the centrifugal device, the washing device, the culture device, the water storage device and the liquid storage device.
5. The stem cell culture perfusion apparatus of claim 4, further comprising:
the temperature control device is arranged inside the box body;
a temperature detection element disposed inside the case;
the control device is electrically connected with the temperature control device and the temperature detection element respectively.
6. The stem cell culture perfusion apparatus of claim 4, further comprising:
and the atomizing element is arranged at the second end of the third water outlet pipe and is electrically connected with the control device.
7. The stem cell culture perfusion apparatus of claim 4, further comprising:
the drying device comprises an air supply pipe, the first end of the air supply pipe is connected with the drying device, the second end of the air supply pipe is connected with the upper end of the crushing device, and the drying device is electrically connected with the control device.
8. The stem cell culture perfusion apparatus of claim 4, further comprising:
a first check valve disposed at a first end of the first connection pipe;
the second one-way valve is arranged at the first end of the second connecting pipe;
a third check valve disposed at a first end of the third connection pipe;
a fourth check valve disposed at a first end of the fourth connection pipe;
a fifth check valve disposed at a first end of the fifth connection pipe;
a sixth check valve disposed at a first end of the sixth connection pipe;
the seventh one-way valve is arranged at the first end of the first water outlet pipe;
the eighth one-way valve is arranged at the first end of the second water outlet pipe;
the ninth one-way valve is arranged at the first end of the third water outlet pipe;
the tenth one-way valve is arranged at the first end of the liquid outlet pipe;
the eleventh one-way valve is arranged at the first end of the discharge pipe;
the control device is electrically connected to the first check valve, the second check valve, the third check valve, the fourth check valve, the fifth check valve, the sixth check valve, the seventh check valve, the eighth check valve, the ninth check valve, the tenth check valve, and the eleventh check valve, respectively.
9. The stem cell culture perfusion apparatus of claim 1, wherein the reaction device is disposed below the size reduction device;
the filtering device is arranged below the reaction device;
the centrifugal device is arranged on the side of the filtering device;
the washing device is arranged on the side of the centrifugal device;
the culture device is arranged on the side of the washing device;
the water storage device is arranged above the crushing device;
the liquid storage device is arranged above the culture device.
10. The stem cell culture perfusion apparatus of claim 9, further comprising:
the first water pump is arranged on the third connecting pipe;
the second water pump is arranged on the fourth connecting pipe;
the third water pump is arranged on the fifth connecting pipe;
and the fourth water pump is arranged on the sixth connecting pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020325600.6U CN212025373U (en) | 2020-03-16 | 2020-03-16 | Stem cell culture perfusion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020325600.6U CN212025373U (en) | 2020-03-16 | 2020-03-16 | Stem cell culture perfusion device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212025373U true CN212025373U (en) | 2020-11-27 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020325600.6U Active CN212025373U (en) | 2020-03-16 | 2020-03-16 | Stem cell culture perfusion device |
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