CN212016810U

CN212016810U - Nanofiber membrane for chromatography and chromatography device using nanofiber membrane

Info

Publication number: CN212016810U
Application number: CN202020233712.9U
Authority: CN
Inventors: 贾建东; 陈选; 卢红星
Original assignee: Hangzhou Kebaite Technology Co ltd
Current assignee: Hangzhou Kebaite Technology Co ltd
Priority date: 2020-02-29
Filing date: 2020-02-29
Publication date: 2020-11-27
Anticipated expiration: 2030-02-28

Abstract

The utility model relates to a nanofiber membrane for chromatography, including a plurality of filter unit, range upon range of setting between the filter unit, every filter unit includes thick nanofiber layer of one deck and the thin nanofiber layer of one deck at least, the thick nanofiber layer of adjacent conflict forms thick nanofiber group, the thin nanofiber layer of adjacent conflict forms thin nanofiber group, the unilateral or both sides of thin nanofiber layer are provided with thick nanofiber layer or thick nanofiber group/the unilateral or both sides of thin nanofiber group are provided with thick nanofiber layer or thick nanofiber group. The utility model discloses still relate to a chromatography device who uses this membrane preparation. The utility model aims to achieve the purpose of providing a nanofiber chromatographic membrane and the chromatography device of using this membrane that the structure is more stable, more durable, efficiency is higher.

Description

Nanofiber membrane for chromatography and chromatography device using nanofiber membrane

Technical Field

The utility model relates to a chromatographic device of chromatographic membrane and applied this membrane, especially a chromatographic device that is used for the nanofiber membrane of chromatography and applied this membrane.

Background

In the existing field of purification and separation, chromatography is a common method. Wherein the chromatography method comprises column chromatography, paper chromatography, membrane chromatography, thin layer chromatography, high performance liquid chromatography, etc. In all of these methods, the performance of the chromatographic carrier is a main factor determining the chromatographic effect, and therefore, the chromatographic carrier needs to be optimized to achieve a better chromatographic effect.

Nanofiber membranes, with the potential for high specific surface area and high mass transfer rate, have the potential to offer significant advantages in flux over flat sheet membranes while still having high binding capacity, and thus have attracted some researchers' attention. In addition, in the research, we also find that after the fine nano-fiber raw membrane without the stable structure is subjected to chromatography functionalization modification, the fine nano-fiber chromatographic membrane is easy to swell due to poor mechanical strength when a target liquid passes through the chromatographic membrane, so that staggered pores between fibers are enlarged, the target liquid cannot be well in complete contact with the surface of the chromatographic membrane, only less than 20% of the area of the chromatographic membrane can be utilized, and the effective utilization rate is greatly reduced.

SUMMERY OF THE UTILITY MODEL

The utility model aims to achieve the purpose of providing a nanofiber chromatographic membrane and the chromatography device of using this membrane that the structure is more stable, more durable, efficiency is higher.

In order to achieve the above purpose, the utility model adopts the following technical scheme: the utility model provides a nanofiber membrane for chromatography, includes a plurality of filter unit, range upon range of setting between the filter unit, every filter unit includes thick nanofiber layer of one deck and the thin nanofiber layer of one deck at least, the thick nanofiber layer of adjacent conflict forms thick nanofiber group, the thin nanofiber layer of adjacent conflict forms thin nanofiber group, the unilateral or both sides on thin nanofiber layer are provided with thick nanofiber layer or thick nanofiber group/the unilateral or both sides of thin nanofiber group are provided with thick nanofiber layer or thick nanofiber group.

Further, the loading capacity of the nanofiber membrane is between 30mg/ml and 200mg/ml, and the flow rate is between 1CV/min and 340 CV/min.

Furthermore, the thick nanofiber layer, the thin nanofiber layer, the thick nanofiber group and the thin nanofiber group in the filtering unit are arranged in a stacked mode.

Further, the diameter of the fiber in the coarse nanofiber layer is between 1000nm and 8000nm, and the diameter of the fiber in the fine nanofiber layer is between 10nm and 1000 nm.

Further, the total thickness of the coarse nanofiber layer or coarse nanofiber group is set between 50 μm and 9600 μm; the total thickness of the fine nanofiber layer or fine nanofiber group is set between 20 μm and 5000 μm.

Further, the thickness ratio of the fine nanofiber layer and the coarse nanofiber layer, or the fine nanofiber layer and the coarse nanofiber group, or the fine nanofiber group and the coarse nanofiber layer, or the fine nanofiber group and the coarse nanofiber group is between 1/150 and 60.

Furthermore, a coarse nanofiber layer is arranged on the liquid inlet side of the filtering unit.

Furthermore, the filtering unit comprises a fine nanofiber layer, and a coarse nanofiber group is arranged on the liquid inlet side of the filtering unit.

Furthermore, the filtering unit comprises a fine nanofiber layer, and two coarse nanofiber layers are respectively arranged on two sides of the fine nanofiber layer.

Furthermore, the filtering unit comprises a fine nanofiber group, and thick nanofiber groups are respectively arranged on two sides of the fine nanofiber group.

The utility model also provides an use chromatography device of above-mentioned nanofiber membrane preparation, including casing and nanofiber membrane, the casing includes casing and lower casing, go up the casing and form the accommodation space who holds the nanofiber membrane down between the casing, the surface of going up the casing is provided with the feed liquor mouth of pipe, the surface of casing is provided with out the liquid mouth of pipe down, feed liquor mouth of pipe, accommodation space and go out the intercommunication setting between the liquid mouth of pipe.

Furthermore, a plurality of annular grooves are formed in the inner side wall of the upper shell, and a plurality of annular grooves are also formed in the corresponding positions of the inner side wall of the lower shell.

Furthermore, the adjacent annular grooves are uniformly distributed at equal intervals and have the same width.

Furthermore, a flow channel is radially arranged on the inner side wall of the upper shell, and the flow channel is also arranged at the position corresponding to the inner side wall of the lower shell.

Furthermore, the flow passages are uniformly distributed on the inner surfaces of the upper shell and the lower shell in a radial shape.

Further, the depth of the flow channel on the upper shell is the same as that of the annular groove on the upper shell; the depth of the flow channel on the lower shell is the same as that of the annular groove on the lower shell.

Furthermore, the outer edges of the upper shell and the lower shell are also provided with covered edges for fixing the upper shell and the lower shell.

The utility model discloses a nanofiber membrane for chromatography compares prior art, and the advantage that has lies in: 1. the membrane structure is stable and is not easy to swell. 2. Even if some swelling occurs, the chromatographic effect on the whole membrane is very slight. 3. When the chromatography liquid enters the chromatography membrane, the liquid is distributed more uniformly, so that the utilization rate of the surface area of the membrane is higher. The utility model provides a chromatography device compares prior art, and the advantage that has lies in: 1. the service life is longer. 2. The chromatography efficiency is higher.

Drawings

The present invention will be further explained with reference to the accompanying drawings:

FIG. 1 is a schematic structural view of a middle chromatography device according to the present invention;

FIG. 2 is a schematic view of the combination of the upper and lower casings of the middle chromatography device of the present invention;

fig. 3 is a bottom view of the upper case of the present invention;

fig. 4 is a top view of the lower housing of the present invention;

fig. 5 is a schematic view of a first embodiment of the present invention;

fig. 6 is a schematic view of a second embodiment of the present invention;

fig. 7 is a schematic diagram of a deformation situation in a first embodiment of the present invention.

In the figure: 1. an upper housing; 2. a lower housing; 3. edge covering; 4. an accommodating space; 5. a liquid inlet pipe orifice; 6. a liquid outlet pipe orifice; 7. an annular groove; 8. a flow channel; 9. a coarse nanofiber layer; 10. a fine nanofiber layer.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention more clearly understood, the present invention will be described in further detail with reference to the accompanying drawings and detailed description. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those specifically described herein, and therefore the scope of the present invention is not limited by the specific embodiments disclosed below.

The first embodiment is as follows:

a nanofiber membrane for chromatography as shown in fig. 5, comprising a filtration unit. The filter unit comprises a thick nanofiber layer 9 and a thin nanofiber layer 10 which are laminated. It should be noted that all references to stacking herein and hereinafter to the specification are to be taken as merely physical stacking and do not refer to heat staking, adhesives, and the like. In some other embodiments, a certain variation may be performed on the scheme of this embodiment, a plurality of coarse nanofiber layers 9 may be stacked adjacent to each other to form a coarse nanofiber group, and then stacked on one side of the fine nanofiber layer 10; or a plurality of thick nanofiber layers 9 are adjacently stacked to form a thick nanofiber group, and then are stacked on one side of a thin nanofiber group formed by adjacently stacking a plurality of thin nanofiber layers 10; or one thick nanofiber layer 9 is laminated on one side of a group of thin nanofibers formed by adjacently laminating a plurality of thin nanofiber layers 10. Fig. 7 shows a modification of the first embodiment.

Example two:

a nanofiber membrane for chromatography as shown in fig. 6, comprising a filtration unit. The filter unit comprises two thick nanofiber layers 9 and one thin nanofiber layer 10 which are laminated. In example two, the fine nanofiber layer 10 is disposed between two coarse nanofiber layers 9. In some other embodiments, a certain variation may be made to the present embodiment, which may be a fine nanofiber layer 10, on both sides of which a coarse nanofiber layer 9 is stacked, or a coarse nanofiber group formed by stacking a plurality of coarse nanofiber layers 9; alternatively, a plurality of fine nanofiber layers 10 may be stacked adjacent to each other to form a fine nanofiber group, and a coarse nanofiber layer 9 or a coarse nanofiber group formed by stacking a plurality of coarse nanofiber layers 9 adjacent to each other may be stacked adjacent to each other on both sides of the fine nanofiber group.

Example three:

a nanofiber membrane for chromatography comprises a plurality of filtering units, and each adjacent filtering unit is stacked. The plurality of filter units mentioned in this embodiment may be any one or more of embodiments one to three. Preferably, at least one of the two surfaces of the entire nanofiber membrane is a layer 9 of coarse nanofibers or a group of coarse nanofibers.

The difference between the first and third embodiments is that only the coarse nanofiber layer 9 and the fine nanofiber layer 10 are stacked differently to form different nanofiber membranes for chromatography. In all examples, the loading of nanofiber membrane was between 30mg/ml and 200mg/ml and the flow rate was between 1CV/min and 340 CV/min. It should be noted here that the flow rate for chromatographic membranes is related to the membrane thickness, so we say that the nanofiber membrane flow rate is measured at a chromatographic membrane thickness of 2 mm. Further, we define the diameter of the thick nanofibers that make up the nanofiber layer and the diameter of the thin nanofibers that make up the thin nanofiber layer 10, the diameter of the thick nanofibers is defined between 1000nm and 8000nm, and the diameter of the thin nanofibers is defined between 10nm and 1000 nm. It should be noted here that the nanofiber membrane is a broad concept and may include cellulose acetate nanocellulose membrane, regenerated cellulose nanocellulose membrane, polyester nanocellulose membrane, polysulfone nanocellulose membrane, and other derivatives and mixtures thereof.

Further, the total thickness of the coarse nanofiber layer 9 or coarse nanofiber group is set between 50 μm and 9600 μm; the total thickness of the fine nanofiber layer 10 or the fine nanofiber group is set between 20 μm and 5000 μm. Here we make a relevant explanation of this total thickness, where the total thickness of the coarse nanofiber layer 9 or coarse nanofiber group should be the sum of the thickness of all coarse nanofiber layers 9 plus the thickness of all coarse nanofiber groups in the finished film as a whole; the same is true for the total thickness of the fine nanofiber layer 10 or fine nanofiber group. And on the basis of the above thickness, the thickness ratio of the fine nanofiber layer 10 and the coarse nanofiber layer 9, or the fine nanofiber layer 10 and the coarse nanofiber group, or the fine nanofiber group and the coarse nanofiber layer 9, or the fine nanofiber group and the coarse nanofiber group is further between 1/150 and 60. It should be noted that the thickness ratio referred to herein is a ratio of the total thickness of the fine nanofiber layer 10 to the total thickness of the coarse nanofiber layer 9, or is a ratio of the total thickness of the fine nanofiber layer 10 to the total thickness of the coarse nanofiber group, or is a ratio of the total thickness of the fine nanofiber group to the total thickness of the coarse nanofiber layer 9, or is a ratio of the total thickness of the fine nanofiber group to the total thickness of the coarse nanofiber group.

Through analysis, the nanofiber membrane formed by laminating the coarse nanofiber layer 9 (or group) and the fine nanofiber layer 10 (or group) has better strength compared with the traditional pure fine nanofiber membrane; compared with the traditional pure crude nanofiber membrane, the membrane has better chromatography effect. The coarse nanofiber layer 9 has more stable mechanical strength than the fine nanofiber layer 10, i.e., is not easily deformed, and is not easily swollen when a liquid passes through or is soaked (i.e., the pore diameter on the chromatographic membrane is increased); the mechanical strength of the fine nanofiber layer 10 is lower than that of the coarse nanofiber layer 9, and the fine nanofiber layer is easily deformed and swells when a liquid passes through or is soaked (namely, the pore diameter on the chromatographic membrane is increased, so that the contact area between a target liquid and the surface of the membrane is reduced, and the effect is influenced). Furthermore, the thick nanofiber layer 9 and the thin nanofiber layer 10 are mixed to form the nanofiber membrane in the scheme, and the nanofiber membrane equivalently combines the advantages of the thick nanofiber layer 9 and the thin nanofiber layer 10. When the thin nanofiber layer 10 is deformed and swelled by passing a liquid through the thin nanofiber layer 10 during use, the thick nanofiber layer 9 is supported on one side or both sides thereof, and the space in which the thin nanofiber layer 10 swells can be restricted. The performance effect of the nanofiber membrane in the present solution is further defined by defining the thickness, relative thickness, etc. of the coarse nanofiber layer 9 and the fine nanofiber layer 10. On the other hand, if the thick nanofiber layer 9 is provided on one side or both sides, even if the thin nanofiber layer 10 is slightly swollen, the thin fibers of the thin nanofiber layer may extend into the thick nanofiber layer 9 (i.e., the thin fibers of the thin nanofiber layer 10 are filled in the gaps of the thick nanofiber layer 9), and thus, from the overall viewpoint, the overall nanofiber membrane still has a relatively small pore size, so that the nanofiber membrane can still be sufficiently contacted with the target liquid, and the effective contact area is not significantly reduced.

Further, a coarse nanofiber layer 9 is arranged on the liquid inlet side of the filtering unit. When the nanofiber membrane is subjected to chromatography, a chromatography liquid enters from one surface of the membrane, then passes through the membrane body, and flows out from the other surface of the membrane, and a chromatography substance is left on the membrane body. Due to the structure of the chromatographic apparatus, the inlet port is typically a small area orifice that is directed against the surface of the chromatographic membrane. During the liquid entering process, due to the pressure, the liquid entering the membrane cannot be uniformly distributed on the whole surface of the membrane, but quickly passes through the chromatographic membrane in a small range, so that the chromatographic effect of the part of the liquid is poor. The coarse nanofiber layer 9 has a slightly larger pore size than the fine nanofiber layer 10, so that the chromatography liquid can be more rapidly distributed on the whole surface of the membrane after entering the coarse nanofiber layer, and the coarse nanofiber layer is arranged on the liquid inlet side of the filtering unit, so that the coarse nanofiber layer 9 plays a role in diffusion and flow guiding compared with the chromatography liquid before entering the fine nanofiber layer 10. The chromatography liquid can be distributed on the surface of the chromatographic membrane more quickly and uniformly.

As shown in fig. 1 to 4, a chromatography device using the nanofiber membrane is also included in the present application. The chromatography device comprises a shell and a nanofiber membrane, wherein the shell comprises three parts: go up casing 1, lower casing 2 and set up in last casing 1 and lower casing 2 outward flange and be used for fixing in the bordure 3 of an organic whole with last casing 1 and lower casing 2. The upper casing 1 and the lower casing 2 are substantially disc-shaped, when the upper casing 1 is matched with the lower casing 2, a containing space 4 is formed between the upper casing 1 and the lower casing 2, and a nanofiber membrane is arranged in the containing space 4. The middle of the disc-shaped upper shell 1 is provided with a liquid inlet pipe orifice 5, the middle of the disc-shaped lower shell 2 is provided with a liquid outlet pipe orifice 6, and after the assembly is completed, the liquid inlet pipe orifice 5, the liquid outlet pipe orifice 6 and the accommodating space 4 inside the shell are communicated.

In this embodiment, we make relevant settings for the structure of the accommodating space 4, and have a plurality of annular grooves 7 opened on the inner side wall of the upper housing 1, and also have a plurality of annular grooves 7 opened at the corresponding positions on the inner side wall of the lower housing 2. The volume in the accommodating space 4 can be increased by the arrangement of the annular groove 7, so that a nanofiber membrane with a larger surface area can be placed in the accommodating space, and the chromatography efficiency is improved. And the width of the annular grooves 7 at two places is the same, is concentric distribution between every annular groove 7 simultaneously, further sets up the interval between every annular groove 7 on the upper casing 1 or lower casing 2 to the equidistance for annular groove 7 evenly distributed is in whole accommodation space 4, and make full use of whole accommodation space 4 makes chromatography liquid evenly fully distribute in being located the nanofiber membrane in accommodation space 4 more. Meanwhile, radial flow channels 8 are radially formed in the accommodating space 4, and the depth of each flow channel 8 is kept to be the same as that of the corresponding annular groove 7, so that uniform flow of chromatography liquid is facilitated.

The preferred embodiments of the present invention have been described in detail, but it should be understood that various changes and modifications can be made by those skilled in the art after reading the above teaching of the present invention. Such equivalents are intended to fall within the scope of the claims appended hereto.

Claims

1. A nanofiber membrane for chromatography comprising a plurality of filtration units, characterized in that: range upon range of setting between the filter unit, every filter unit includes thick nanofiber layer of one deck and the thin nanofiber layer of one deck at least, adjacent conflict thick nanofiber layer forms thick nanofiber group, adjacent conflict thin nanofiber layer forms thin nanofiber group, the unilateral or both sides on thin nanofiber layer are provided with thick nanofiber layer or thick nanofiber group/the unilateral or both sides of thin nanofiber group are provided with thick nanofiber layer or thick nanofiber group.

2. The nanofiber membrane for chromatography as claimed in claim 1, wherein: the loading capacity of the nanofiber membrane is between 30mg/ml and 200mg/ml, and the flow rate is between 1CV/min and 340 CV/min.

3. The nanofiber membrane for chromatography as claimed in claim 1, wherein: the thick nanofiber layer, the thin nanofiber layer, the thick nanofiber group and the thin nanofiber group in the filtering unit are arranged in a stacked mode.

4. The nanofiber membrane for chromatography according to claim 1, wherein the diameter of the fiber in the coarse nanofiber layer is between 1000nm and 8000nm, and the diameter of the fiber in the fine nanofiber layer is between 10nm and 1000 nm.

5. The nanofiber membrane for chromatography according to claim 1, wherein the total thickness of the coarse nanofiber layer or coarse nanofiber group is set between 50 μ ι η -9600 μ ι η; the total thickness of the fine nanofiber layer or fine nanofiber group is set between 20 μm and 5000 μm.

6. The nanofiber membrane for chromatography according to claim 4, wherein the thickness ratio of the fine nanofiber layer and the coarse nanofiber layer, or the fine nanofiber layer and the coarse nanofiber group, or the fine nanofiber group and the coarse nanofiber layer, or the fine nanofiber group and the coarse nanofiber group is between 1/150-60.

7. The nanofiber membrane for chromatography as claimed in claim 2, wherein a coarse nanofiber layer is disposed on the liquid inlet side of the filtration unit.

8. The nanofiber membrane for chromatography as claimed in claim 2, wherein the filtration unit comprises a fine nanofiber layer, and the filtration unit is provided with a coarse nanofiber group on a liquid inlet side.

9. The nanofiber membrane for chromatography as claimed in claim 2, wherein the filtration unit comprises a fine nanofiber layer, and a coarse nanofiber layer is respectively disposed on both sides of the fine nanofiber layer.

10. The nanofiber membrane for chromatography as claimed in claim 2, wherein the filtration unit comprises a fine nanofiber group, and a coarse nanofiber group is respectively disposed at both sides of the fine nanofiber group.

11. A chromatography device using the nanofiber membrane for chromatography as claimed in claim 1, comprising a housing and the nanofiber membrane, wherein: the casing includes casing and lower casing, go up the casing and form the accommodation space who holds the nanofiber membrane down between the casing, the surface of going up the casing is provided with the feed liquor mouth of pipe, the surface of casing is provided with out the liquid mouth of pipe down, feed liquor mouth of pipe, accommodation space and play intercommunication setting between the liquid mouth of pipe.

12. The chromatography device of claim 11, wherein: a plurality of annular grooves are formed in the inner side wall of the upper shell, and a plurality of annular grooves are also formed in the corresponding positions of the inner side wall of the lower shell.

13. The chromatography device of claim 12, wherein: equal-distance evenly distributed and annular groove width are the same between adjacent annular groove.

14. The chromatography device of claim 12, wherein: the inner side wall of the upper shell is radially provided with a flow channel, and the position, corresponding to the inner side wall of the lower shell, of the lower shell is also provided with the flow channel.

15. The chromatography device of claim 14, wherein: the flow passages are uniformly distributed on the inner surfaces of the upper shell and the lower shell in a radial shape.

16. The chromatography device of claim 14, wherein: the depth of the flow channel on the upper shell is the same as that of the annular groove on the upper shell; the depth of the flow channel on the lower shell is the same as that of the annular groove on the lower shell.

17. The chromatography device of claim 11, wherein: the outer edges of the upper shell and the lower shell are also provided with covered edges for fixing the upper shell and the lower shell.