CN211999734U - Escherichia coli detection device in environmental sample - Google Patents
Escherichia coli detection device in environmental sample Download PDFInfo
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- CN211999734U CN211999734U CN202020239589.1U CN202020239589U CN211999734U CN 211999734 U CN211999734 U CN 211999734U CN 202020239589 U CN202020239589 U CN 202020239589U CN 211999734 U CN211999734 U CN 211999734U
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- escherichia coli
- environmental sample
- detection cup
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Abstract
The utility model discloses a can realize a plurality of environment sample mesocolibacillus detection device that detect technology step goes on same equipment. The device for detecting the escherichia coli in the environmental sample comprises a base, a rotary table and a fixed block; a detection cup is arranged on the fixed block; a heating device for heating the detection cup is arranged in the fixed block; a top plate is arranged above the detection cup; a top cover is arranged below the top plate; a lifting rod is arranged between the top plate and the base; a sterile water storage box and a culture medium storage box are arranged above the top plate; a first liquid adding pipe communicated with the sterile water storage tank and a second liquid adding pipe communicated with the culture medium storage tank are arranged below the top plate; a stirring device is arranged below the top plate; a first driving motor for driving the stirring device is arranged above the top plate; the base is provided with a detection box. The device for detecting the escherichia coli in the environmental sample can prevent the sample from being polluted in the detection process, and can simplify the operation.
Description
Technical Field
The utility model relates to an environment sampling test equipment, especially an escherichia coli detection device in environmental sample.
Background
It is well known that: escherichia coli, also known as Escherichia coli, was discovered in 1885. Escherichia coli is a conditional pathogen, and can cause gastrointestinal tract infection or urinary tract infection of various local tissues and organs of human and various animals under certain conditions.
Escherichia coli in the environment can be hidden in soil for a long time and can enter vegetables, so that the health of human beings is affected. Therefore, the escherichia coli in the environmental sample needs to be detected, so that the pollution of the escherichia coli on vegetables and grain crops is avoided.
The existing escherichia coli detection device generally needs to be detected through several steps, so that the detected sample is easily polluted in the interval process of the two steps, and the detection accuracy is reduced.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that a can realize that a plurality of detection technology steps go on same equipment is provided, avoid the sample to receive the pollution in the testing process to escherichia coli detection device in the environmental sample that can the simplified operation.
The utility model provides a technical scheme that its technical problem adopted is: the device for detecting the escherichia coli in the environmental sample comprises a base, wherein a rotary table is arranged on the base; a fixed block is fixedly arranged on the rotary table; a detection cup is arranged on the fixed block;
a heating device for heating the detection cup is arranged in the fixed block; a clamping device for clamping the detection cup is arranged on the fixed block; a top plate is arranged above the detection cup; a top cover is arranged below the top plate; the top cover is fixedly connected with the top plate;
a lifting rod is arranged between the top plate and the base; a locking bolt is arranged on the lifting rod; a sterile water storage tank and a culture medium storage tank are arranged above the top plate;
a first liquid adding pipe communicated with the sterile water storage tank and a second liquid adding pipe communicated with the culture medium storage tank are arranged below the top plate;
a first valve is arranged on the first liquid adding pipe; a second valve is arranged on the second liquid adding pipe;
the first liquid adding pipe and the second liquid adding pipe penetrate through the top cover; the top cover is positioned right above the detection cup;
a stirring device extending into the detection cup is arranged below the top plate; a first driving motor for driving the stirring device is arranged above the top plate; a second driving motor for driving the rotary table is arranged at the bottom of the base; the base is provided with a detection box.
Specifically, the clamping device comprises a fixing plate, an adjusting bolt and a clamping block; the adjusting bolt penetrates through the fixing plate to tightly push the clamping block on the detection cup.
Preferably, the heating device is a resistance heating device.
Further, the stirring device adopts a stirring rod; and a temperature sensor is arranged on the stirring rod.
Preferably, the first valve and the second valve are both solenoid valves.
Furthermore, the detection cup is a transparent cup and is provided with scale marks.
The utility model has the advantages that: environmental sample in escherichia coli detection device, owing to can realize that the solution of sample in the testing process dilutes, the mixture of solvent and culture medium, cultivates and heating temperature's control, consequently can make a plurality of processes in the testing process go on same equipment to can simplify the detection, improve detection efficiency, can simplify the detection operation simultaneously, environmental sample in escherichia coli detection device, simple structure, it is easy and simple to handle, with low costs.
Drawings
FIG. 1 is a schematic structural diagram of an apparatus for detecting Escherichia coli in an environmental sample according to an embodiment of the present invention;
the following are marked in the figure: 1-base, 2-rotary table, 3-fixed block, 4-heating device, 5-lifting rod, 6-locking bolt, 7-fixed plate, 8-adjusting bolt, 9-clamping block, 10-detection cup, 11-stirring device, 12-top cover, 13-first liquid adding pipe, 14-second liquid adding pipe, 15-top plate, 16-sterile water storage box, 17-culture medium storage box, 18-first driving motor, 19-first valve, 20-second valve, 21-detection box and 22-second driving motor.
Detailed Description
The present invention will be further explained with reference to the drawings and examples.
As shown in fig. 1, the device for detecting escherichia coli in an environmental sample of the present invention comprises a base 1, wherein a turntable 2 is arranged on the base 1; a fixed block 3 is fixedly arranged on the rotary table 2; a detection cup 10 is arranged on the fixed block 3;
a heating device 4 for heating the detection cup 10 is arranged in the fixed block 3; a clamping device for clamping the detection cup 10 is arranged on the fixed block 3; a top plate 15 is arranged above the detection cup 10; a top cover 12 is arranged below the top plate 15; the top cover 12 is fixedly connected with the top plate 15;
a lifting rod 5 is arranged between the top plate 15 and the base 1; the lifting rod 5 is provided with a locking bolt 6; a sterile water storage tank 16 and a culture medium storage tank 17 are arranged above the top plate 15;
a first liquid adding pipe 13 communicated with a sterile water storage tank 16 and a second liquid adding pipe 14 communicated with a culture medium storage tank 17 are arranged below the top plate 15;
a first valve 19 is arranged on the first liquid adding pipe 13; a second valve 20 is arranged on the second filling pipe 14;
the first liquid adding pipe 13 and the second liquid adding pipe 14 both penetrate through the top cover 12; the top cover 12 is positioned right above the detection cup 10;
a stirring device 11 extending into the detection cup 10 is arranged below the top plate 15; a first driving motor 18 for driving the stirring device 11 is arranged above the top plate 15; a second driving motor 22 for driving the rotary table 2 is arranged at the bottom of the base 1; the base 1 is provided with a detection box 21.
In the application process: the top plate 15 is first raised by the lifting lever 5 so that the stirring device 11 leaves the detection cup 10; then adding a sample into the detection cup; fixing the detection cup 10 on the fixing block 3 through a clamping device; the lifting rod 5 is lowered, so that the stirring device 11 extends into the detection cup 10, the top cover 12 is positioned right above the detection cup 10, and then sterile water is adjusted into the detection cup 10 through the first liquid adding pipe 13 to dilute the sample; and the detection cup 10 is heated by the heating device 4 at the bottom, and simultaneously, the culture medium is added through the second liquid adding pipe 14; and starting the stirring device 11 to realize stirring and mixing of the sample solution and the culture medium.
Boiling in water for 10min, standing at normal temperature for 30-40 min for sufficient renaturation, and centrifuging for 1min by starting a rotary table to obtain supernatant; the supernatant is put into the cartridge 21 for detection.
To sum up, environmental sample in escherichia coli detection device, owing to can realize the control of solution dilution, the mixture of solvent and culture medium, cultivation and heating temperature of sample in the testing process, consequently can make a plurality of processes in the testing process go on same equipment to can simplify the detection, improve detection efficiency, can simplify the detection operation simultaneously, environmental sample in escherichia coli detection device, simple structure, easy and simple to handle, it is with low costs.
In order to simplify the structure, specifically, the clamping device comprises a fixing plate 7, an adjusting bolt 8 and a clamping block 9; the adjusting bolt 8 penetrates through the fixing plate 7 to tightly press the clamping block 9 on the detection cup 10.
In order to facilitate the control of the heating device, it is preferable that the heating device 4 is a resistance heating device.
In order to facilitate monitoring and detecting the temperature in the cup 10, further, the stirring device 11 adopts a stirring rod; and a temperature sensor is arranged on the stirring rod.
For the convenience of control, it is preferable that the first valve 19 and the second valve 20 are solenoid valves.
In order to facilitate observation, the amount of the sterile water and the culture medium is added, and further, the detection cup 10 is a transparent cup and is provided with scale marks.
Claims (6)
1. Escherichia coli detection device in environmental sample, its characterized in that: the device comprises a base (1), wherein a rotary table (2) is arranged on the base (1); a fixed block (3) is fixedly arranged on the rotary table (2); a detection cup (10) is arranged on the fixed block (3);
a heating device (4) for heating the detection cup (10) is arranged in the fixed block (3); a clamping device for clamping the detection cup (10) is arranged on the fixed block (3); a top plate (15) is arranged above the detection cup (10); a top cover (12) is arranged below the top plate (15); the top cover (12) is fixedly connected with the top plate (15);
a lifting rod (5) is arranged between the top plate (15) and the base (1); a locking bolt (6) is arranged on the lifting rod (5); a sterile water storage tank (16) and a culture medium storage tank (17) are arranged above the top plate (15);
a first liquid adding pipe (13) communicated with a sterile water storage tank (16) and a second liquid adding pipe (14) communicated with a culture medium storage tank (17) are arranged below the top plate (15);
a first valve (19) is arranged on the first liquid adding pipe (13); a second valve (20) is arranged on the second liquid adding pipe (14);
the first liquid adding pipe (13) and the second liquid adding pipe (14) both penetrate through the top cover (12); the top cover (12) is positioned right above the detection cup (10);
a stirring device (11) extending into the detection cup (10) is arranged below the top plate (15); a first driving motor (18) for driving the stirring device (11) is arranged above the top plate (15); a second driving motor (22) for driving the rotary table (2) is arranged at the bottom of the base (1); the base (1) is provided with a detection box (21).
2. The apparatus for detecting Escherichia coli in an environmental sample according to claim 1, wherein: the clamping device comprises a fixing plate (7), an adjusting bolt (8) and a clamping block (9); the adjusting bolt (8) penetrates through the fixing plate (7) to tightly push the clamping block (9) on the detection cup (10).
3. The apparatus for detecting Escherichia coli in an environmental sample according to claim 2, wherein: the heating device (4) adopts a resistance heating device.
4. The apparatus for detecting Escherichia coli in an environmental sample according to claim 2, wherein: the stirring device (11) adopts a stirring rod; and a temperature sensor is arranged on the stirring rod.
5. The apparatus for detecting Escherichia coli in an environmental sample according to claim 2, wherein: the first valve (19) and the second valve (20) both adopt electromagnetic valves.
6. The apparatus for detecting Escherichia coli in an environmental sample according to claim 2, wherein: the detection cup (10) is a transparent cup and is provided with scale marks.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020239589.1U CN211999734U (en) | 2020-02-27 | 2020-02-27 | Escherichia coli detection device in environmental sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020239589.1U CN211999734U (en) | 2020-02-27 | 2020-02-27 | Escherichia coli detection device in environmental sample |
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| Publication Number | Publication Date |
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| CN211999734U true CN211999734U (en) | 2020-11-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020239589.1U Active CN211999734U (en) | 2020-02-27 | 2020-02-27 | Escherichia coli detection device in environmental sample |
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| CN (1) | CN211999734U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112763302A (en) * | 2020-12-24 | 2021-05-07 | 黄文锋 | Sample quantification and quick detection equipment for food quality detection |
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2020
- 2020-02-27 CN CN202020239589.1U patent/CN211999734U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112763302A (en) * | 2020-12-24 | 2021-05-07 | 黄文锋 | Sample quantification and quick detection equipment for food quality detection |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: G7-5, science and technology innovation center, Liangjiang New Area, 74 Yunhe Road, Beibei District, Chongqing Patentee after: Zhongke Testing Technology Service (Chongqing) Co.,Ltd. Address before: G7-5, science and technology innovation center, Liangjiang New Area, 74 Yunhe Road, Beibei District, Chongqing Patentee before: CHONGQING CAS TESTING TECHNOLOGY SERVICES Co.,Ltd. |
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| CP01 | Change in the name or title of a patent holder |