CN211871976U - Manganese oxidizing bacteria and biological manganese oxide culture device - Google Patents
Manganese oxidizing bacteria and biological manganese oxide culture device Download PDFInfo
- Publication number
- CN211871976U CN211871976U CN202020258900.7U CN202020258900U CN211871976U CN 211871976 U CN211871976 U CN 211871976U CN 202020258900 U CN202020258900 U CN 202020258900U CN 211871976 U CN211871976 U CN 211871976U
- Authority
- CN
- China
- Prior art keywords
- oxidation reaction
- reaction tank
- tank
- gas
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a manganese oxidizing bacteria and biological manganese oxide's culture apparatus, including expanding the culture tank, oxidizing reaction jar, the liquid detection system who is connected with expanding the culture tank and oxidizing reaction jar and the gas circulation system who communicates with expanding the culture tank and oxidizing reaction jar, be provided with the oxygen flow distribution plate in the expanding culture tank, be provided with the draft tube that has the sieve mesh in the oxidizing reaction jar, be provided with bubble cap formula drainage tube in the draft tube, the bottom is provided with the aeration head that communicates with the oxidizing reaction jar air inlet in the draft tube; in the reaction process, the whole device is in a closed state, the problem of strain pollution caused by invasion of mixed bacteria in the culture and oxidation reaction processes can be avoided, the culture operation is carried out by using the device, the culture time is low, the strain aging rate is low, the strain quality is high, the reaction energy consumption is low, the gas-liquid mixing effect and the circulation rate are improved, the gas-liquid distribution is uniform, the gas-liquid mass transfer performance is higher, and the operation cost can be effectively reduced.
Description
Technical Field
The utility model belongs to the technical field of environmental science and engineering, concretely relates to manganese oxidizing bacteria and biological manganese oxide's culture apparatus.
Background
The manganese oxidizing bacteria exist in various environments in a wide variety of ways, but the mechanisms of manganese (II) oxide of different manganese oxidizing bacteria are different, and particularly, the generated biological manganese oxide has different structural characteristics and physicochemical properties. The biological manganese oxide is an important component of manganese oxide in nature, and has the characteristics of weak crystallization, small particle size, high oxidation-reduction potential, large specific surface area and the like. It is an important strong oxidant in the environment and oxidation of organic pollutants is believed to significantly affect the environmental behavior of organic pollutants. At present, researches on biological manganese oxide oxidized organic pollutants mainly focus on the aspect of sewage treatment, the oxidation mechanism of different manganese oxidizing bacteria on manganese (II) is explored, and the theoretical system for producing the biological manganese oxide by the manganese oxidizing bacteria is continuously supplemented and perfected, so that the important significance is realized on optimizing the sewage treatment by the biological manganese oxide oxidized organic pollutants. Since the provision of stable manganese oxidizing bacteria and biological oxides of manganese is a prerequisite for research, research on culture devices for manganese oxidizing bacteria and biological oxides of manganese is urgent.
At present, research on the generation of biological manganese oxide by manganese oxidizing bacteria is mainly carried out in laboratories, but experimental devices such as shake flasks, beakers, conical flasks and the like are mostly adopted, and due to the lack of corresponding matching devices for sampling and other operations, the continuity of culture is poor, the operation is complex, the pollution probability is high, and the accuracy of experimental results is greatly influenced. The airlift reactor is a multiphase flow reactor which realizes the circular flow of water by taking gas as driving force and does not need mechanical stirring and pump lifting. The airlift reactor has the characteristics of simple structure, easy maintenance, low energy consumption, good mixing performance, low shearing stress, high interphase mass transfer and heat transfer efficiency and the like, is widely applied to various fields of bioengineering, energy chemical engineering, environmental protection and the like, and in addition, the airlift reactor avoids shaft sealing, realizes the circular flow of gas-liquid-solid mixture only by the pushing of air flow, and is widely applied to the culture and biodegradation experiments of various single floras.
For example, chinese patent publication No. CN 202039060U discloses a reactor for the amplified culture of mineral leaching bacteria, which uses the gas lift action to drive the liquid to flow, realizes the automatic circulation of the bacterial liquid, and is suitable for the culture of various mineral leaching bacteria, but does not consider the problem of solid formation in the mineral leaching microorganism system, neglects the bacteria aging problem and the balance relationship between cell loss and cell growth in the continuous culture process, and has the problem of too large height-diameter ratio (H/D).
Patent CN 101016584A provides a leaching mode suitable for a microorganism metallurgy tank, which is characterized in that a plurality of guide cylinders are adopted in an airlift reactor, the problem of overlarge height-diameter ratio (H/D) of the airlift reactor is solved, the ore leaching bioreactor has the advantages of low abrasion degree to microorganism thallus and easy large-scale production, can be used for culturing biological ore leaching and ore leaching bacteria, but does not consider the problems of discharge and blockage caused by easy solid formation in an ore leaching microorganism system.
Patent CN 107881081A provides a leaching microorganism continuous enlarging culture device and culture method for maintaining high-concentration bacteria liquid continuous production and simultaneously effectively relieving the loss of strains in a reactor. The method is characterized in that the detachable multilayer filler is arranged in the airlift reactor, a multilayer fluidized bed system can be formed, and the problem of microorganism loss is solved by immobilized culture of microorganisms in a culture device. However, this device involves the problem of replacing the packing, and directly discharges air into the environment, is liable to cause contamination of the bacterial species, and is only suitable for bacteria forming a biofilm.
The patent CN 106430599A discloses a method for treating dye wastewater by using marine aspergillus niger mycelium pellets and an airlift reactor, wherein the reactor can intermittently treat multiple batches of dye wastewater, so that the mycelium pellets are fully utilized, the treatment cost is reduced, and the treatment effect of the dye wastewater is improved. However, the method needs to add mycelium pellets in each batch of treatment process to treat the next batch of dye wastewater after the mycelium pellets recover the decoloring capacity, and the treatment efficiency is low.
SUMMERY OF THE UTILITY MODEL
The utility model aims to overcome the problems and provide a manganese oxidizing bacteria and biological manganese oxide culturing device. The device realizes the culture of manganese oxidizing bacteria and biological manganese oxide by using series airlift reactors, and has the characteristics of high efficiency, low power consumption and simple operation
In order to achieve the above purpose, the technical scheme of the utility model is that:
a manganese oxidizing bacteria and biological manganese oxide culture device comprises an expansion culture tank, an oxidation reaction tank, a liquid detection system connected with the expansion culture tank and the oxidation reaction tank, and a gas circulation system communicated with the expansion culture tank and the oxidation reaction tank;
the enlarged culture tank is a hollow hemispherical tank body, the top and the bottom of the enlarged culture tank are hollow hemispheres, the middle part of the enlarged culture tank is a hollow cylindrical tank body, the top of the enlarged culture tank is provided with a No. 1 pressure gauge, a No. 1 safety valve and an air outlet of the enlarged culture tank, the upper part of the cylindrical part of the enlarged culture tank is provided with an enlarged culture tank feed liquid port with a No. 4 valve for injecting culture medium into the enlarged culture tank, the lower part of the feed liquid port of the enlarged culture tank is provided with an enlarged culture tank microorganism inlet with a No. 7 valve for injecting strains into the enlarged culture tank, the upper part of the cylindrical part of the enlarged culture tank is also provided with a microorganism discharge port for conveying bacteria liquid into the oxidation reaction tank, the bottom of the enlarged culture tank is provided with an air inlet of the enlarged culture tank with a No. 1 one-way valve, the lower part of the cylindrical part of the enlarged culture tank is provided with, a No. 1 heating and heat-insulating outer layer is arranged between the microorganism inlet of the expanded culture tank and the liquid outlet of the expanded culture tank and is wrapped on the outer surface of the tank body of the expanded culture tank.
The oxidation reaction tank is a hollow cylindrical tank body with a plane top surface, a hollow cylindrical upper part and a hollow conical lower part, the top of the oxidation reaction tank is provided with a No. 2 pressure gauge, a No. 2 safety valve, an oxidation reaction tank air outlet and an oxidation reaction tank feed liquid port with a No. 8 valve and used for injecting a culture medium into the oxidation reaction tank, the upper part of the cylindrical part of the oxidation reaction tank is provided with an oxidation reaction tank microorganism inlet with a No. 6 valve and used for inputting bacterial liquid output by a microorganism discharge port of an expanded culture tank into the oxidation reaction tank, the lower part of the cylindrical part of the oxidation reaction tank is provided with an oxidation reaction tank liquid discharge port with a No. 3 valve and used for discharging the liquid in the oxidation reaction tank, the lower part of the oxidation reaction tank is provided with an oxidation reaction tank air inlet with a No. 2 one-way valve, and the oxidation reaction tank air inlet is communicated with the inner cavity of the oxidation reaction tank, the tip of the conical part at the lower part of the oxidation reaction tank is provided with a solid-liquid discharge pipe with a No. 1 valve, and a No. 2 heating and heat-preserving outer layer is arranged between a microorganism inlet of the oxidation reaction tank and a liquid discharge port of the oxidation reaction tank and is wrapped on the outer surface of the tank body of the oxidation reaction tank;
the microorganism outlet of the enlarged culture tank is communicated with the microorganism inlet of the oxidation reaction tank through a pipeline with a microorganism supply pump and a No. 6 valve, the microorganism supply pump is close to one side of the microorganism outlet of the enlarged culture tank, and the No. 6 valve is close to one side of the microorganism inlet of the oxidation reaction tank;
the liquid detection system comprises a data acquisition box, two groups of electrode groups and two electromagnetic contactors, wherein each group of electrode group comprises an anticorrosion temperature probe, a pH electrode, an oxidation-reduction electrode and a dissolved oxygen electrode, four test holes are respectively arranged above the middle part of the enlarged culture tank and the upper part of the oxidation reaction tank, the anticorrosion temperature probes of the two groups of electrode groups, the pH electrode, the redox electrode and the dissolved oxygen electrode are respectively inserted into four testing holes of the corresponding expansion culture tank or four testing holes of the oxidation reaction tank to be contacted with liquid in the tank, two electromagnetic contactors are respectively connected with the No. 1 heating and heat-preserving outer layer and the No. 2 heating and heat-preserving outer layer, the two electromagnetic contactors are respectively connected with the data acquisition box through cables, and the anti-corrosion temperature probe, the pH electrode, the redox electrode and the dissolved oxygen electrode in each group of electrode groups are all connected with the data acquisition box through cables;
the gas circulation system comprises a hollow gas-liquid separation bottle and an air compressor, wherein the top of the hollow gas-liquid separation bottle is provided with a gas inlet, a gas outlet, a No. 3 pressure gauge and a No. 3 safety valve, the bottom of the gas-liquid separation bottle is provided with a gas-liquid separation bottle liquid outlet with a No. 5 valve, the gas outlet of the gas-liquid separation bottle is communicated with the gas inlet of the air compressor through a pipeline, the gas outlet of the air compressor is communicated with a No. 1 one-way valve of the gas inlet of the expanded culture tank and a No. 2 one-way valve of the gas inlet of the oxidation reaction tank through a gas flowmeter and a pipeline, the gas inlet of the gas-liquid separation bottle is communicated with the gas outlet of the expanded culture tank and the gas outlet of the oxidation reaction tank through a gas circulation pipe, and the pipeline between the gas inlet of, a No. 4 one-way valve is arranged on a pipeline of the gas circulating pipe between the gas outlet of the amplification culture tank and the gas outlet of the oxidation reaction tank.
The further technical scheme comprises the following steps:
the gas outlet of the amplification culture tank is communicated with the gas circulation pipe through a No. 1 condensing pipe, and the gas outlet of the oxidation reaction tank is communicated with the gas circulation pipe through a No. 2 condensing pipe.
The expansion culture tank is an airlift reaction tank, a disc-shaped oxygen flow distribution plate is horizontally arranged at the bottom of an inner cavity of the expansion culture tank, and a plurality of flow distribution holes with uniformly distributed through hole structures are formed in the oxygen flow distribution plate.
The oxidation reaction tank is an airlift reaction tank, the oxidation reaction tank adopts a multi-guide-cylinder air circulation type structure, at least 3 guide cylinders which are uniformly distributed at intervals are arranged in an inner cavity of the oxidation reaction tank, the guide cylinders are fixed in the inner cavity of the upper part of the oxidation reaction tank through a plurality of support rods which are horizontally and fixedly arranged on the inner wall of the upper part of the oxidation reaction tank, the height of each guide cylinder is 70 percent of the height of the cylindrical part of the oxidation reaction tank and is lower than the height of the liquid level in the cylindrical part of the oxidation reaction tank, the ratio of the height to the diameter of the cylindrical part of the oxidation reaction tank is 1-1.5, the area of the cross section of the cylindrical part of the oxidation reaction tank is 1.5-2 times of the sum of the cross sections of all the guide cylinders, the ratio of the height to the diameter of the guide cylinders is greater than 3, a sieve hole group is arranged on the wall surface, the sieve hole group consists of 1-8 sieve hole layers, each sieve hole layer consists of sieve holes with through hole structures which are the same in number and are uniformly distributed at intervals along the circumferential direction of the guide cylinder, the diameters of the sieve holes are 5-15 mm, the bottom of an inner cavity of the guide cylinder is provided with an aeration head, all the aeration heads are communicated with an air inlet of the oxidation reaction tank through a pipeline, a bubble cap type drainage tube is arranged between the guide cylinder and the aeration head and arranged in the bottom of the inner cavity of the guide cylinder for enhancing gas-liquid mixing, the bubble cap type drainage tube is integrally welded by a cylindrical hollow tube and a baffle cover which is 20-50 mm above the cylindrical hollow tube through a fulcrum, a gap of 20-50 mm is formed between the cylindrical hollow tube and the baffle cover for gas-liquid flowing, the aeration head is communicated with an air tube, the height of the bubble cap type drainage tube is 1/4-1/2 of the height of the cylindrical part of the oxidation reaction tank, and the, the bubble cap type drainage tube is fixed in an inner cavity of the guide cylinder through a plurality of support rods which are horizontally and fixedly arranged on the inner wall of the guide cylinder, the bottom end of the bubble cap type drainage tube is 10-50mm higher than the bottom end of the guide cylinder during installation, the baffle is of a hollow conical structure, the conical angle of the baffle is 60-120 degrees, and the conical angle of the conical structure at the lower part of the oxidation reaction tank is 45-55 degrees.
The structure of the No. 1 heating heat preservation outer layer is the same as that of the No. 2 heating heat preservation outer layer, the No. 1 heating heat preservation outer layer and the No. 2 heating heat preservation outer layer are formed by a radiating metal foil, a self-temperature-limiting heating belt and a porous foam heat preservation belt from the inner part to the outer part of a tank wall which is tightly attached to the corresponding tank wall in sequence, and the electromagnetic contactor is connected with the self-temperature-limiting heating belt.
The utility model has the advantages that:
1. the process is more optimized, and the whole device is in a closed state, so that the problem of strain pollution caused by invasion of mixed bacteria in the culture and oxidation reaction processes can be avoided;
2. the oxidation reaction of the cultured strains and the strain is separated, the culture time is reduced, the strain aging rate is slow, the liquid strain obtained by culture can be recycled, and the strain quality is high;
3. the method adopts a mode of aeration and stirring, compressed air is used as liquid to promote power to promote the material macroscopic mixing and mass transfer processes, gas-liquid substance diffusion exchange is utilized, stirring equipment is omitted, the energy consumption of a reactor is reduced, and the abrasion of thalli is reduced. Meanwhile, because the system maintains pressure slightly higher than normal standard atmospheric pressure, the oxygen content is further improved;
4. the multi-guide-cylinder type gas lift reactor is adopted, the height-diameter ratio (H/D) of the tank body is reduced, the multi-guide-cylinder is improved, the sieve pores and the bubble cap type drainage tubes are added, the gas-liquid mixing effect and the circulation rate are improved, the gas-liquid distribution is uniform, and the gas-liquid mass transfer performance is higher; in addition, the existence of solids in the reaction process is fully considered, the bubble cap type drainage tube is arranged to protect the aeration head, and the bottom of the tank body is conical to facilitate the sedimentation of the solids during standing;
5. the gas circulation system is adopted, so that the tail gas can be recycled, the sealing effect of the device is facilitated, and the operation cost is reduced;
6. the self-temperature-limiting electric heating belt is adopted, the temperature during heating can be automatically limited, and the output power can be automatically adjusted along with the temperature of a heated system;
7. and a data acquisition system is adopted, so that the monitoring of liquid properties in the culture and oxidation reaction processes is facilitated, and the mechanism of biological manganese oxide generated by manganese oxidizing bacteria is further known.
Drawings
FIG. 1 is a schematic structural diagram of the present invention
In the figure: 100. an expanded culture tank, 101, a feed liquid port of the expanded culture tank, No. 102.1 heating and heat-preserving outer layer, 103, an oxygen splitter plate, 104, a liquid discharge port of the expanded culture tank, 105, a microorganism supply pump, No. 106.1 pressure gauge, No. 107.1 safety valve, 108, an air inlet of the expanded culture tank, No. 109.1 condenser pipe, 110, an air outlet of the expanded culture tank, 111, a microorganism inlet of the expanded culture tank, 112, a microorganism discharge port, 200, an oxidation reaction tank, 201, a feed liquid port of the oxidation reaction tank, No. 202.2 heating and heat-preserving outer layer, 203, 204, a liquid discharge port of the oxidation reaction tank, 205, a bubble-cap type drainage tube, No. 206.2 pressure gauge, No. 207.2 safety valve, 208, an air inlet of the oxidation reaction tank, No. 209.2 condenser pipe, 210, an air outlet of the oxidation reaction tank, 211, an inlet of the oxidation reaction tank, 212, a solid-liquid discharge pipe, 213, a, 300. the liquid detection system comprises a liquid detection system, 301 an electrode group, 302 a data acquisition box, 303 an electromagnetic contactor, 400 an air circulation system, 402 an air circulation pipe, 403 a gas-liquid separation bottle, 404 a gas-liquid separation bottle liquid discharge port, 405 an air compressor, 406.3 a pressure gauge, 407.3 a safety valve, 408 a flowmeter, V1.1 a check valve, V2.2 a check valve, V3.1 a valve, V4.2 a valve, V5.3 a valve, V6.4 a valve, V7.5 a check valve, V8.3 a check valve, V9.4 a check valve, V10.6 a valve, V11.7 a valve and V12.8 a valve.
Detailed Description
The experimental novel features will be described in further detail with reference to the accompanying drawings so that those skilled in the art can implement the invention with reference to the description:
in fig. 1, the manganese oxidizing bacteria and biological manganese oxide culturing apparatus according to the present invention includes an amplification culture tank 100, an oxidation reaction tank 200, a liquid detection system 300 connected to the amplification culture tank 100 and the oxidation reaction tank 200, and a gas circulation system 400 communicating with the amplification culture tank 100 and the oxidation reaction tank 200.
At present, it is considered that Mn (II) may have toxicity to some microorganisms, the microorganisms oxidize Mn (II) to prevent toxicity accumulation, and continuous subculture in a nutrient solution containing Mn (II) is easy to cause changes of physiological and biochemical properties of strains and aging of the strains, and the like, and a tandem device of an enlarged culture tank 100 and an oxidation reaction tank 200 is arranged in the embodiment for culture. Wherein the enlarged culture tank 100 contains Mn (II) -free culture medium and enlarged culture manganese oxidizing bacteria, and is connected with the oxidation reaction tank 200, after the bacteria in the enlarged culture tank 100 are in logarithmic growth phase, the bacteria in logarithmic growth phase are injected into the oxidation reaction tank 200 through a communicating pipe between the enlarged reaction tank 100 and the oxidation reaction tank 200, the culture medium containing Mn (II) is added into the oxidation reaction tank 200, after reaction for a period of time, biological manganese oxide is obtained, solid and liquid are discharged, and then sterile buffer solution is injected to flush the oxidation reaction tank 200.
The amplification culture tank 100 is a hollow hemispherical tank body with a hollow top and a hollow bottom, the middle part is a cylindrical barrel shape, the top of the amplification culture tank 100 is provided with a No. 1 pressure gauge 106, a No. 1 safety valve 107 and an amplification culture tank air outlet 110, the upper part of the cylindrical barrel shape part of the amplification culture tank 100 is provided with an amplification culture tank feed liquid port 101 with a No. 4 valve V6 for injecting culture medium into the amplification culture tank 100, the lower part of the amplification culture tank feed liquid port 101 is provided with an amplification culture tank microorganism inlet 111 with a No. 7 valve V11 for injecting strains into the amplification culture tank 100, the upper part of the cylindrical barrel shape part of the amplification culture tank 100 is also provided with a microorganism discharge port 112 for delivering bacteria liquid into the oxidation reaction tank 200, the bottom of the amplification culture tank 100 is provided with an amplification culture tank air inlet 108 with a No. 1 check valve V1, and the lower part of the cylindrical barrel shape part of the amplification culture tank 100 is provided with amplification culture tank liquid with a No. 2 valve V4 A No. 1 heating and heat-preserving outer layer 102 is arranged between the microorganism inlet 111 of the expanded culture tank and the liquid outlet 104 of the expanded culture tank and is wrapped on the outer surface of the expanded culture tank 100.
The expansion culture tank 100 is an airlift reaction tank, a disc-shaped oxygen distribution plate 103 is horizontally arranged at the bottom of an inner cavity of the expansion culture tank 100, and a plurality of uniformly distributed through hole structure distribution holes are formed in the oxygen distribution plate 103, so that gas can be uniformly distributed, larger bubbles can be eliminated, the gas-liquid mixing efficiency can be improved, and the stirring effect can be enhanced.
The oxidation reaction tank 200 is a hollow cylindrical tank body with a plane top surface, a hollow upper part and a hollow lower part, the oxidation reaction tank 200 is provided with a No. 2 pressure gauge 206 at the top part, a No. 2 safety valve 207, an oxidation reaction tank air outlet 210 and an oxidation reaction tank feed liquid port 201 with a No. 8 valve V12 for injecting culture medium into the oxidation reaction tank 200, the oxidation reaction tank 200 is provided with an oxidation reaction tank microorganism inlet 211 with a No. 6 valve V10 for inputting bacterial liquid output from a microorganism discharge port 112 of the expanded culture tank 100 into the oxidation reaction tank 200 at the upper part of the cylindrical part, the oxidation reaction tank 200 is provided with an oxidation reaction tank liquid discharge port 204 with a No. 3 valve V5 for discharging liquid in the oxidation reaction tank 200 at the lower part of the cylindrical part, the oxidation reaction tank 200 is provided with an oxidation reaction tank air inlet 208 with a No. 2 check valve V2 at the lower part, an air inlet 208 of the oxidation reaction tank is communicated with an inner cavity of the oxidation reaction tank 200, a tip part of a conical part at the lower part of the oxidation reaction tank 200 is provided with a solid-liquid discharge pipe 212 with a No. 1 valve V3, and a No. 2 heating and heat-preserving outer layer 202 is arranged between a microorganism inlet 211 of the oxidation reaction tank and a liquid discharge port 204 of the oxidation reaction tank and is wrapped on the outer surface of the tank body of the oxidation reaction tank 200;
the oxidation reaction tank 200 is an airlift reaction tank, the oxidation reaction tank 200 adopts a multi-guide-cylinder air circulation type structure, the reactor takes air as liquid lifting power to promote the material macro-mixing and mass transfer processes, and because the aeration head is positioned under the guide cylinder, almost all air enters the guide cylinder, so that the average density of fluid inside the guide cylinder is greatly lower than that of fluid outside the guide cylinder, and the existence of the pressure difference generates the power of fluid circulation flow.
At least 3 guide cylinders 213 which are uniformly distributed at intervals are arranged in an inner cavity of the oxidation reaction tank 200, the guide cylinders 213 are fixed in the inner cavity of the upper part of the oxidation reaction tank 200 through a plurality of support rods which are horizontally and fixedly arranged on the inner wall of the upper part of the oxidation reaction tank 200, the height of each guide cylinder 213 is 70 percent of the height of the cylindrical part of the oxidation reaction tank 200 and is lower than the liquid level height in the cylindrical part of the oxidation reaction tank 200, the ratio of the height to the diameter of the cylindrical part of the oxidation reaction tank 200 is 1-1.5, the area of the cross section of the cylindrical part of the oxidation reaction tank 200 is 1.5-2 times of the sum of the cross sections of all the guide cylinders 213, and the ratio of the height to the diameter of.
The upper end wall surface of the guide cylinder 213 is provided with a sieve hole group, and the sieve holes can improve the flow track and enhance the gas-liquid mixing effect. The height of sieve pore group is 1/5 to 1/8 of draft tube height, and sieve pore group comprises 1 ~ 8 layers of sieve pore layer, and every layer of sieve pore layer comprises the same and along the sieve pore 203 of the through-hole structure of draft tube 213 circumferencial direction interval evenly distributed of quantity, and the diameter of sieve pore 203 is 5 ~ 15 mm.
The bottom of the inner cavity of the guide cylinder 213 is provided with an aeration head 214, all the aeration heads 214 are communicated with an oxidation reaction tank air inlet 208 through pipelines, a bubble-cap type drainage pipe 205 is arranged in the bottom of the inner cavity of the guide cylinder 213 between the guide cylinder 213 and the aeration heads 214 for enhancing gas-liquid mixing, and the bubble-cap type drainage pipe 205 can prevent the aeration heads from being blocked by solids when the aeration is stopped for sedimentation. The bubble cap type drainage tube 205 is formed by welding a cylindrical hollow tube and a baffle cover 20-50 mm above the cylindrical hollow tube into a whole through a supporting point, a gap 20-50 mm is formed between the cylindrical hollow tube and the baffle cover to allow gas and liquid to flow, an aeration head 214 is communicated with a gas tube 215, the height of the bubble cap type drainage tube 205 is 1/4-1/2 of the height of the cylindrical barrel-shaped part of the oxidation reaction tank 200, the diameter of the bubble cap type drainage tube 205 is 3/11-7/11 of the diameter of the guide barrel 213, the bubble cap type drainage tube 205 is fixed in the inner cavity of the guide barrel 213 through a plurality of supporting rods horizontally and fixedly arranged on the inner wall of the guide barrel 213, the bottom end of the bubble cap type drainage tube 205 is 10-50mm higher than the bottom end of the guide barrel 213 during installation, the baffle cover is of a hollow conical structure.
In order to collect the biological manganese oxide generated in the oxidation reaction tank 200, the taper angle of the conical structure at the lower part of the oxidation reaction tank 200 is 45-55 degrees.
The microorganism outlet 112 of the amplification culture tank 100 is communicated with the microorganism inlet 211 of the oxidation reaction tank 200 through a pipeline with a microorganism supply pump 105 and a No. 6 valve V10, wherein the microorganism supply pump 105 is close to one side of the microorganism outlet 112 of the amplification culture tank 100, and the No. 6 valve V10 is close to one side of the microorganism inlet 211 of the oxidation reaction tank 200.
The liquid detection system 300 comprises a data acquisition box 302, two groups of electrode sets 301 and two electromagnetic contactors 303, wherein each group of electrode sets 301 comprises an anticorrosion temperature probe, a pH electrode, an oxidation-reduction electrode and a dissolved oxygen electrode, four test holes (only two test holes are shown in figure 1) are arranged above the middle part of the amplification culture tank 100 and above the upper part of the oxidation reaction tank 200, the anticorrosion temperature probes, the pH electrodes, the oxidation-reduction electrodes and the dissolved oxygen electrodes of the two groups of electrode sets 301 are respectively inserted into the corresponding four test holes (only two test holes are shown in figure 1) of the amplification culture tank 100 or the four test holes (only two test holes are shown in figure 1) of the oxidation reaction tank 200 to be contacted with liquid in the tank, the two electromagnetic contactors 303 are respectively connected with a No. 1 heating and heat-preserving outer layer 102 and a No. 2 heating and heat-preserving outer layer 202, the two electromagnetic contactors 303 are respectively connected with the data acquisition box 302 through cables, the anti-corrosion temperature probe, the pH electrode, the oxidation-reduction electrode and the dissolved oxygen electrode in each group of electrode groups 301 are all connected with the data acquisition box 302 through cables.
The structure of the No. 1 heating and heat-preserving outer layer 102 is the same as that of the No. 2 heating and heat-preserving outer layer 202, the No. 1 heating and heat-preserving outer layer 102 and the No. 2 heating and heat-preserving outer layer 202 are sequentially composed of a heat-dissipating metal foil, a self-temperature-limiting heating belt and a porous foam heat-preserving belt from inside to outside from the close adhesion to the corresponding tank wall, and the electromagnetic contactor 303 is connected with the self-temperature-limiting heating belt. The electromagnetic contactor can receive a command from the data acquisition box to realize the adjustment of the temperature of the self-temperature-limiting heating belt.
The gas circulation system 400 comprises a hollow gas-liquid separation bottle 403 with a gas inlet at the top, a gas outlet, a No. 3 pressure gauge 406 and a No. 3 safety valve 407, and an air compressor 405, wherein the bottom of the gas-liquid separation bottle 403 is provided with a gas-liquid separation bottle liquid outlet 404 with a No. 5 valve V7, the gas outlet of the gas-liquid separation bottle 403 is communicated with the gas inlet of the air compressor 405 through a pipeline, at the moment, the air is humid air which can further reduce the volatilization of the liquid in the tank, the gas outlet of the air compressor 405 is communicated with the gas inlet 108 of the expanded culture tank through a pipeline, the gas outlet of the air compressor 405 is communicated with a No. 1 one-way valve V1 of the gas inlet 108 of the expanded culture tank and a No. 2 one-way valve V2 of the gas inlet 208 of the oxidation reaction tank through a gas flow meter 408 and a pipeline, and the gas, and the gas circulation pipe 402 introduced into the gas inlet of the gas-liquid separation bottle 403 should be immersed in the liquid in the gas-liquid separation bottle 403 which is filled with part of sterile water in advance, so that a small amount of gas-liquid mixture in the gas circulation pipe can realize gas-liquid separation, a No. 3 one-way valve V8 is arranged on the pipeline of the gas circulation pipe 402 between the gas inlet of the gas-liquid separation bottle 403 and the gas outlet 110 of the amplification culture tank, and a No. 4 one-way valve V9 is arranged on the pipeline of the gas circulation pipe 402 between the gas outlet 110 of the amplification culture tank and the gas outlet 210 of the oxidation reaction tank, so that the liquid in the gas-liquid separation bottle can be prevented from flowing back to the. The gas outlet 110 of the amplification culture tank is communicated with the gas circulation pipe 402 through a No. 1 condensing pipe 109, and the gas outlet 210 of the oxidation reaction tank is communicated with the gas circulation pipe 402 through a No. 2 condensing pipe 209, so that the volatilization of liquid in the reaction process can be prevented, the liquid is condensed and flows back into the bodies of the oxidation reaction tank 200 and the amplification reaction tank 100, and the contamination of mixed bacteria can be prevented.
The gas circulation system 400 may employ an air supply or an oxygen supply. At present, the research considers that most of manganese oxidizing bacteria belong to aerobic bacteria, the whole system for culturing the liquid strains maintains the pressure of 0.1-0.4Mpa, so that the oxygen content of water can be improved, and the dissolved oxygen concentration (DO) in the oxidation reaction tank 200 and the amplification reaction tank 100 is more than 2ppm, which is beneficial to improving the activity of the liquid strains. Although pure oxygen condition culture can make the liquid spawn active, the oxygen supply is expensive, and the air compressor 405 is selected for air supply in the example.
The culture method of the utility model is as follows:
(1) preparation work: the connection of the culture device is confirmed to be complete, each valve is ensured to be in a normally closed state, the pressure gauge works normally, and the whole system is ensured to be kept in a closed state.
(2) And (3) amplification culture:
opening a No. 7 valve V11 to inject strains to be expanded from an expanded culture tank microorganism inlet hole 111 of an expanded reaction tank 100, opening a No. 4 valve V6 to inject corresponding Mn (II) -free culture medium from a feed liquid port 101 of the expanded culture tank, opening an air compressor 405 and a No. 1 one-way valve V1, leading air to enter the expanded culture tank 100 through a flow meter 408, an expanded culture tank air inlet 108 and an oxygen flow distribution plate 103 to start ventilation and stirring, simultaneously opening a No. 3 one-way valve V8, leading tail gas to enter a gas circulation pipe 402 through an expanded reaction tank air outlet 110 and a No. 1 condenser 109, and leading the tail gas to enter the air compressor 405 again after gas-liquid separation in a gas-liquid separation bottle 403.
The liquid detection system 300 detects the liquid properties (pH, temperature, dissolved oxygen, oxidation-reduction potential), and supplements the culture medium or the acid-base adjusting solution from the feed liquid port 101 of the expansion culture tank at any time to make the culture solution in the optimal state for bacterial growth all the time.
After culturing for a period of time, the No. 2 valve V4 is opened to collect a small part of liquid by enlarging the liquid discharge port 104 of the culture tank to detect the bacterial OD600Value (absorbance of bacteria liquid when wavelength of spectrophotometer is 600 nm), after confirming culture is in logarithmic growth phase, discharging 20% bacteria liquid from microorganism discharge port 112 to oxidation reaction tank 200 for continuous oxidation reaction, opening No. 2 valve V4 to discharge 20% bacteria liquid through liquid discharge port 104 of amplification reaction tank for other property study, opening No. 4 valve V6 to reinject Mn (II) -free culture medium from liquid port 101 of amplification culture tank and continuous amplification culture with the rest bacteria liquid.
(3) And (3) oxidation reaction:
opening a No. 6 valve V10 on a communication pipeline between the oxidation reaction tank 200 and the expansion reaction tank 100, introducing a bacterial liquid from a microorganism discharge port 112 of the expansion reaction tank 100 to an oxidation reaction tank microorganism inlet 211 through a microorganism supply pump 105, and opening a No. 8 valve V12 to inject a corresponding Mn (II) -containing culture medium from an oxidation reaction tank feed liquid port 201. Opening a No. 2 one-way valve V2 to enable air to enter the oxidation reaction tank 200 from an oxidation reaction tank air inlet 208, enabling the air to enter the bubble-cap type drainage tube 205 through the aeration head 214, enabling the air to flow into the guide cylinder 213 after the bubble-cap type drainage tube 205 is subjected to advanced gas-liquid mixing, and generating power for fluid circulating flow by utilizing the average density difference of fluid inside and outside the guide cylinder 213. Meanwhile, the guide cylinder 213 is also provided with sieve holes 203 to improve the flow path and enhance the gas-liquid mixing effect. Meanwhile, a No. 4 one-way valve V9 is opened, so that the tail gas enters the gas circulation pipe 402 through the gas outlet 210 of the oxidation reaction tank and the No. 2 condenser 209.
The liquid properties (pH, temperature, dissolved oxygen, oxidation-reduction potential) are detected by the liquid detection system 300
(4) And (3) precipitation: and (3) generating biological manganese oxide after reacting for a period of time, closing a No. 2 check valve V2 and a No. 4 check valve V9 to stop ventilation, standing for a period of time, performing solid-liquid separation, precipitating solids to the bottom of a cone, opening a No. 1 valve V3 to discharge the solids through a solid-liquid discharge pipe 212, closing a No. 1 valve V3 and opening a No. 3 valve V5 to discharge the liquids through an oxidation reaction tank liquid discharge port 204, and closing a No. 1 valve V3 and a No. 3 valve V5 after discharging the liquids.
(5) Cleaning: the valve V12 No. 8 is reopened, and sterile buffer solution is injected into the oxidation reaction tank 200 from the oxidation reaction tank inlet 201 for washing, and the valve V3 No. 1 is reopened to discharge waste liquid through the solid-liquid discharge pipe 212.
(6) And (3) recycling: and (5) repeating the processes (3) to (5).
Claims (5)
1. The manganese oxidizing bacteria and biological manganese oxide culture device is characterized by comprising an expansion culture tank (100), an oxidation reaction tank (200), a liquid detection system (300) connected with the expansion culture tank (100) and the oxidation reaction tank (200), and a gas circulation system (400) communicated with the expansion culture tank (100) and the oxidation reaction tank (200);
the expanded culture tank (100) is a hollow hemispherical tank body with a hollow hemispherical top and a hollow cylindrical middle part, the top of the expanded culture tank (100) is provided with a No. 1 pressure gauge (106), a No. 1 safety valve (107) and an expanded culture tank air outlet (110), the upper part of the cylindrical part of the expanded culture tank (100) is provided with an expanded culture tank feed liquid port (101) which is provided with a No. 4 valve (V6) and is used for injecting culture medium into the expanded culture tank (100), the lower part of the expanded culture tank feed liquid port (101) is provided with an expanded culture tank microorganism inlet (111) which is provided with a No. 7 valve (V11) and is used for injecting strains into the expanded culture tank (100), the upper part of the cylindrical part of the expanded culture tank (100) is also provided with a microorganism discharge port (112) for conveying bacterium liquid into the oxidation reaction tank (200), and the bottom of the expanded culture tank (100) is provided with an expanded culture tank (108) with a No. 1 one-way valve (V1) ) The lower part of the cylindrical part of the expanded culture tank (100) is provided with an expanded culture tank liquid outlet (104) with a No. 2 valve (V4), and a No. 1 heating and heat-preserving outer layer (102) is arranged between a microorganism inlet (111) of the expanded culture tank and the expanded culture tank liquid outlet (104) and is wrapped on the outer surface of the tank body of the expanded culture tank (100);
the oxidation reaction tank (200) is a hollow cylindrical tank body with a plane top surface, a hollow cylindrical upper part and a hollow conical lower part, the top of the oxidation reaction tank (200) is provided with a No. 2 pressure gauge (206), a No. 2 safety valve (207), an oxidation reaction tank air outlet (210) and an oxidation reaction tank feed liquid port (201) with a No. 8 valve (V12) for injecting a culture medium into the oxidation reaction tank (200), the upper part of the cylindrical part of the oxidation reaction tank (200) is provided with a No. 6 valve (V10) for inputting bacterial liquid output by a microorganism discharge port (112) of the enlarged culture tank (100) into an oxidation reaction tank microorganism inlet (211) in the oxidation reaction tank (200), the lower part of the cylindrical part of the oxidation reaction tank (200) is provided with an oxidation reaction tank liquid discharge port (204) with a No. 3 valve (V5) for discharging liquid in the oxidation reaction tank (200), the lower part of the oxidation reaction tank (200) is provided with an oxidation reaction tank air inlet (208) with a No. 2 check valve (V2), the oxidation reaction tank air inlet (208) is communicated with the inner cavity of the oxidation reaction tank (200), the tip of the conical part at the lower part of the oxidation reaction tank (200) is provided with a solid-liquid discharge pipe (212) with a No. 1 valve (V3), and a No. 2 heating and heat-preserving outer layer (202) is arranged between a microorganism inlet (211) of the oxidation reaction tank and a liquid discharge port (204) of the oxidation reaction tank and wraps the outer surface of the tank body of the oxidation reaction tank (200);
the microorganism discharge port (112) of the expanded culture tank (100) is communicated with the microorganism inlet (211) of the oxidation reaction tank (200) through a pipeline with a microorganism supply pump (105) and a No. 6 valve (V10), the microorganism supply pump (105) is close to one side of the microorganism discharge port (112) of the expanded culture tank (100), and the No. 6 valve (V10) is close to one side of the microorganism inlet (211) of the oxidation reaction tank (200);
the liquid detection system (300) comprises a data acquisition box (302), two groups of electrode groups (301) and two electromagnetic contactors (303), wherein each group of electrode groups (301) comprises an anticorrosion temperature probe, a pH electrode, an oxidation-reduction electrode and a dissolved oxygen electrode, four test holes are respectively arranged above the middle part of the amplification culture tank (100) and above the upper part of the oxidation reaction tank (200), the anticorrosion temperature probes, the pH electrodes, the oxidation-reduction electrodes and the dissolved oxygen electrodes of the two groups of electrode groups (301) are respectively inserted into the four test holes of the corresponding amplification culture tank (100) or the four test holes of the oxidation reaction tank (200) to be contacted with liquid in the tank, the two electromagnetic contactors (303) are respectively connected with the No. 1 heating and heat-preserving outer layer (102) and the No. 2 heating and heat-preserving outer layer (202), and the two electromagnetic contactors (303) are respectively connected with the data acquisition box (302) through cables, the anti-corrosion temperature probe, the pH electrode, the oxidation-reduction electrode and the dissolved oxygen electrode in each group of electrode groups (301) are all connected with a data acquisition box (302) through cables;
the gas circulation system (400) comprises a hollow gas-liquid separation bottle (403) and an air compressor (405), wherein the top of the hollow gas-liquid separation bottle is provided with a gas inlet, a gas outlet, a No. 3 pressure gauge (406) and a No. 3 safety valve (407), the bottom of the gas-liquid separation bottle (403) is provided with a gas-liquid separation bottle liquid discharge port (404) with a No. 5 valve (V7), the gas outlet of the gas-liquid separation bottle (403) is communicated with the gas inlet of the air compressor (405) through a pipeline, the gas outlet of the air compressor (405) is communicated with a No. 1 one-way valve (V1) of the gas inlet (108) of the expanded culture tank and a No. 2 one-way valve (V2) of the gas inlet (208) of the oxidation reaction tank through a gas flowmeter (408) and a pipeline, the gas inlet of the gas-liquid separation bottle (403) is communicated with the gas outlet (110) of the expanded culture tank and the gas outlet (210) of the oxidation, a No. 3 one-way valve (V8) is arranged on a pipeline of the gas circulation pipe (402) between the gas inlet of the gas-liquid separation bottle (403) and the gas outlet (110) of the amplification culture tank, and a No. 4 one-way valve (V9) is arranged on a pipeline of the gas circulation pipe (402) between the gas outlet (110) of the amplification culture tank and the gas outlet (210) of the oxidation reaction tank.
2. The apparatus for culturing Mn oxidizing bacteria and biological oxides of manganese according to claim 1, wherein the outlet (110) of the expanded culture vessel is connected to the gas circulation tube (402) via a No. 1 condenser tube (109), and the outlet (210) of the oxidation reaction vessel is connected to the gas circulation tube (402) via a No. 2 condenser tube (209).
3. The apparatus for culturing Mn oxidizing bacteria and biological Mn oxides as claimed in claim 1, wherein the expanded culture tank (100) is an airlift reaction tank, a disc-shaped oxygen distribution plate (103) is horizontally disposed at the bottom of the inner cavity of the expanded culture tank (100), and the oxygen distribution plate (103) has a plurality of distribution holes with a uniformly distributed through hole structure.
4. The apparatus for culturing Mn oxidizing bacteria and biological Mn oxides according to claim 1, wherein the oxidation reaction tank (200) is an airlift reaction tank, the oxidation reaction tank (200) has a multi-draft-tube air circulation structure, at least 3 draft tubes (213) are uniformly distributed at intervals in an inner cavity of the oxidation reaction tank (200), the draft tubes (213) are fixed in the inner cavity of the upper part of the oxidation reaction tank (200) through a plurality of support rods horizontally and fixedly arranged on the inner wall of the upper part of the oxidation reaction tank (200), the height of the draft tubes (213) is 70% of the height of the cylindrical part of the oxidation reaction tank (200) and lower than the liquid level in the cylindrical part of the oxidation reaction tank (200), the ratio of the height to the diameter of the cylindrical part of the oxidation reaction tank (200) is 1-1.5.2 times of the sum of the cross-sectional areas of all the draft tubes (213), the ratio of the height to the diameter of the guide cylinder (213) is more than 3, the upper end wall surface of the guide cylinder (213) is provided with a sieve hole group, the height of the sieve hole group is 1/5-1/8 of the height of the guide cylinder, the sieve hole group consists of 1-8 sieve hole layers, each sieve hole layer consists of sieve holes (203) with the same number and through hole structures which are uniformly distributed along the circumferential direction of the guide cylinder (213) at intervals, the diameter of each sieve hole (203) is 5-15 mm, the bottom of the inner cavity of the guide cylinder (213) is provided with an aeration head (214), all the aeration heads (214) are communicated with an air inlet (208) of an oxidation reaction tank through a pipeline, a bubble cap type drainage tube (205) is arranged between the guide cylinder (213) and the aeration head (214) and is arranged at the bottom of the inner cavity of the guide cylinder (213) for enhancing gas-liquid mixing, the bubble cap type drainage tube (205) is formed by welding a cylindrical hollow tube and a, a gap of 20-50 mm is formed between the cylindrical hollow pipe and the baffle cover to allow gas and liquid to flow, an aeration head (214) is communicated with a gas pipe (215), the height of the bubble-cap type drainage pipe (205) is 1/4-1/2 of the height of the cylindrical part of the oxidation reaction tank (200), the diameter of the bubble-cap type drainage pipe (205) is 3/11-7/11 of the diameter of the guide cylinder (213), the bubble-cap type drainage pipe (205) is fixed in the inner cavity of the guide cylinder (213) through a plurality of support rods horizontally and fixedly arranged on the inner wall of the guide cylinder (213), the bottom end of the bubble-cap type drainage pipe (205) is 10-50mm higher than the bottom end of the baffle cylinder (213) during installation, the baffle cover is of a hollow conical structure, the conical angle of the baffle cover is 60-120 degrees, and the conical angle of the lower conical structure of the oxidation reaction tank (200.
5. The device for culturing the manganese oxidizing bacteria and the biological manganese oxides as claimed in claim 1, wherein the No. 1 heating and heat preserving outer layer (102) and the No. 2 heating and heat preserving outer layer (202) have the same structure, the No. 1 heating and heat preserving outer layer (102) and the No. 2 heating and heat preserving outer layer (202) sequentially comprise a heat dissipation metal foil, a self-temperature-limiting heating belt and a porous foam heat preserving belt from inside to outside from the close contact with the corresponding tank wall, and the electromagnetic contactor (303) is connected with the self-temperature-limiting heating belt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020258900.7U CN211871976U (en) | 2020-03-05 | 2020-03-05 | Manganese oxidizing bacteria and biological manganese oxide culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020258900.7U CN211871976U (en) | 2020-03-05 | 2020-03-05 | Manganese oxidizing bacteria and biological manganese oxide culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211871976U true CN211871976U (en) | 2020-11-06 |
Family
ID=73254658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020258900.7U Expired - Fee Related CN211871976U (en) | 2020-03-05 | 2020-03-05 | Manganese oxidizing bacteria and biological manganese oxide culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211871976U (en) |
-
2020
- 2020-03-05 CN CN202020258900.7U patent/CN211871976U/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5114780B2 (en) | Anaerobic treatment method and apparatus | |
| CN204310864U (en) | The circulation pulling flow type immobilization bioreactor that the pool, a kind of lake water body in-situ is repaired | |
| CN107915320B (en) | Air-floating type half-short-cut nitrification-anaerobic ammonia oxidation reactor | |
| CN105060472B (en) | anaerobic membrane bioreactor | |
| CN101981174A (en) | Method for producing biogas | |
| CN103922468B (en) | Low-energy consumption aeration-free domestic sewage recycling apparatus | |
| CN207684971U (en) | Aerobic inner circulation bio-separation reactor | |
| CN104355400B (en) | Vertical vertical-flow multimedium biological respinse circulation stirring system and device thereof | |
| CN106630147A (en) | Device and method for removing nitrate of water body based on biological membrane reactor | |
| CN105565489A (en) | Sludge circulation efficient hydrolytic reactor | |
| CN109292967B (en) | SBBR (sequencing batch reactor) process-based high-ammonia nitrogen rural biochemical sewage treatment device and method | |
| CN211871976U (en) | Manganese oxidizing bacteria and biological manganese oxide culture device | |
| CN205953996U (en) | Bioreactor | |
| CN112358039A (en) | Moving bed biofilm reactor and sewage treatment system | |
| CN204874012U (en) | Anaerobic membrane bioreactor | |
| CN101428904B (en) | Gas winding cycle stream stirring system for open type oxygen lack pool | |
| CN216639051U (en) | Short-range coupling anaerobic ammonia oxidation denitrification reactor and integrated equipment | |
| CN211521737U (en) | Anaerobic membrane bioreactor | |
| CN209367935U (en) | Adaptive intelligent ACMBR reactor | |
| CN105330017B (en) | A kind of anaerobic reactor and culturing wastewater processing system and method | |
| CN204490680U (en) | A kind of pharmacy wastewater treatment device | |
| CN208577510U (en) | A kind of microkinetic combined film bioreactor | |
| CN201850177U (en) | Vortex flow, laminar flow and pulse flow type anaerobic bioreactor | |
| CN217947837U (en) | Ozone catalytic oxidation treatment device for pig-raising wastewater advanced treatment process | |
| CN212246654U (en) | Breed circulating water treatment and retrieval and utilization integration system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201106 |