CN211861625U

CN211861625U - Storage device of cell freezing protective agent

Info

Publication number: CN211861625U
Application number: CN201922232686.8U
Authority: CN
Inventors: 徐念沁; 付松锋; 姜强
Original assignee: Nanjing Sansheng Biotechnology Co ltd
Current assignee: Nanjing Sansheng Biotechnology Co ltd
Priority date: 2019-12-12
Filing date: 2019-12-12
Publication date: 2020-11-06
Anticipated expiration: 2029-12-12

Abstract

The utility model discloses a storage device of cell cryoprotectant, including cup, bowl cover, extrusion structure, extrusion pipe, bracing piece, discharge pipe, piston, pipe, second spring, sealing plug, egress opening and stirring structure. The utility model has the advantages that: push-and-pull through bracing piece and the piston that is located the extrusion intraduct, make the piston aerify to the cup inside, make the inside atmospheric pressure increase of cup and then extrude inside cell freezing protection liquid by pipe and discharge pipe, and when cell freezing protection liquid is extruded to the sealing plug position, because internal pressure crescent, the sealing plug is promoted gradually, remove to discharge pipe opening direction, finally make the egress opening switch on, cell freezing protection agent can flow through the egress opening, and after internal pressure descends, under the second spring action, the sealing plug replies again, come up the discharge pipe resealing, effectually prevent that discharge pipe department from remaining liquid, avoid causing the pollution to inside solution.

Description

Storage device of cell freezing protective agent

Technical Field

The utility model relates to a storage device specifically is a storage device of cell cryoprotectant, belongs to cell storage technical field.

Background

Cells are the basic structural and functional units of an organism. All organisms except viruses are composed of cells, but the life activities of the viruses must be embodied in the cells, the cell bodies are tiny and can be observed under a microscope, and the shapes of the cells are various. Mainly composed of nucleus and cytoplasm, and has cell membrane on the surface. Higher plants have cell walls outside the cell membrane, plastids in the cytoplasm, chloroplasts and vacuoles in the body, and mitochondria. Animal cells have no cell wall, usually centrosomes in the cytoplasm, and higher plant cells. The cells have the functions of movement, nutrition, reproduction and the like. The cryopreservation of western gymnasium is a main way for preserving cells for a long time, is an important means for researching cells in biology and medicine, and the cells need to be protected by a cell cryoprotectant when being preserved by freezing.

However, when the cell freezing protective solution is stored and taken, the cell freezing protective solution is required to be extracted according to the requirement, the pouring mode is inconvenient to operate, and when the cell freezing protective solution is extruded through an extrusion type vessel, an extrusion port is directly exposed in the air, so that the solution components can be polluted, and the cell freezing storage is influenced.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a storage device of cell freezing protective agent just in order to solve above-mentioned problem, conveniently save and take cell freezing protective agent, can seal extruding the mouth simultaneously, prevent that the protection liquid at extruding the mouth position from receiving the pollution.

The utility model realizes the above purpose by the following technical proposal, a storage device of cell freezing protective agent, which comprises a cup body, one side of the cup body is connected with a cup cover by screw thread, the cup cover is connected with an extrusion structure, the extrusion structure comprises an extrusion pipe, a support rod, a discharge pipe, a piston, a conduit, a second spring, a sealing plug and an outflow port, the extrusion pipe runs through the cup cover, the extrusion pipe is fixedly connected with the cup cover, the interior of the extrusion pipe is connected with the support rod in a sliding way, one end of the support rod is fixedly connected with the piston, the cross section of the piston is in an arc structure, the convex surface of the piston is fixedly connected with one end of the support rod, the discharge pipe is fixedly connected with the cup cover, one end of the discharge pipe is communicated with the discharge pipe, the other end of the guide pipe is located at the bottom of the cup body, the other end of the discharge pipe is connected with the sealing plug in a sliding mode, one end of the sealing plug is fixedly connected with the second spring, the other end of the second spring is fixedly connected to the inner side wall of the discharge pipe, the outflow port is further formed in one end side face of the discharge pipe, one side of the sealing plug is connected with the discharge pipe in a sliding mode through the outflow port, and the middle of the cup cover is provided with a stirring structure.

Preferably, in order to enable the supporting rod to automatically return, multiple times of extrusion can be conveniently carried out, and the extruding structure further comprises a first spring, the first spring is sleeved on the supporting rod, one end of the first spring is fixedly connected with the extruding pipe, and the other end of the first spring is fixedly connected with the supporting rod.

Preferably, in order to press out the solution inside conveniently, the stirring of the solution inside can be carried out through the stirring structure as required simultaneously, the extrusion structure still includes the clamp plate, one side of clamp plate with the one end of bracing piece is rotated and is connected.

Preferably, in order to settle the stirring block and limit the conduit for discharging the solution, the stirring structure comprises a fixing ring, the fixing ring is mounted on the inner side wall of the cup body, and the conduit is clamped with one side of the fixing ring.

Preferably, in order to conveniently stir the internal solution, the solution is always kept in a uniformly stirred state in the extrusion process, the stirring structure further comprises a sliding rod, a screw rod and a stirring block, the sliding rod is connected with the cup cover in a sliding manner, one end of the sliding rod is fixedly connected with the screw rod, the other end of the sliding rod is abutted to the pressing plate, and the screw rod is in threaded connection with the middle of the stirring block.

Preferably, in order to ensure the sealing performance of the overall structure in the sliding process of the sliding rod and enable the sliding rod to reset and be convenient to stir, the stirring structure further comprises a sealing ring and a third spring, the sealing ring is fixedly connected with the cup cover, the sliding rod is connected with the sealing ring in a sliding manner, the sliding rod is further sleeved with the third spring, one end of the third spring is fixedly connected with the cup cover, and the other end of the third spring is fixedly connected with the sliding rod.

Preferably, for the user can say that the slide bar block is on the clamp plate for can carry out the stirring to inside solution when pressing the clamp plate, the stirring structure still includes the draw-in groove, one side of clamp plate still is equipped with the draw-in groove, the draw-in groove be with the circular structure that the slide bar sectional area is the same, just a breach is seted up to one side of draw-in groove, the one end of slide bar is passed through the draw-in groove with clamp plate looks block.

The utility model has the advantages that: the piston is inflated to the inner part of the cup body by pushing and pulling the supporting rod and the piston which are positioned in the extruding pipe, so that the air pressure in the cup body is increased to further press out the cell freezing and protecting liquid in the cup body from the guide pipe and the discharge pipe, and when the cell freezing and protecting liquid is pressed to the sealing plug part, because the internal pressure is gradually increased, the sealing plug is gradually pushed to move towards the opening direction of the discharge pipe, finally the outflow port is conducted, the cell cryoprotectant can flow out through the outflow port, and when the internal pressure is reduced, under the action of the second spring, the sealing plug moves back again to reseal the discharge pipe, effectively prevent the liquid from remaining at the discharge pipe to cause pollution to the solution inside, simultaneously can also stir the solution of inside through the stirring structure that sets up on the bowl cover for the cell refrigeration protection liquid of extruding mixes more evenly.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view of a connection structure of the extrusion structure and the stirring structure shown in FIG. 1;

FIG. 3 is a schematic diagram of the extruded structure shown in FIG. 1;

fig. 4 is a schematic view of a connection structure of the pressing plate and the stirring structure shown in fig. 1.

In the figure: 1. the cup comprises a cup body, 2, a cup cover, 3, an extrusion structure, 31, an extrusion pipe, 32, a support rod, 33, a pressure plate, 34, a discharge pipe, 35, a first spring, 36, a piston, 37, a guide pipe, 38, a second spring, 39, a sealing plug, 39a, an outflow port, 4, a stirring structure, 41, a sliding rod, 42, a sealing ring, 43, a spiral rod, 44, a stirring block, 45, a fixing ring, 46, a third spring, 47 and a clamping groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, a storage device for a cell freezing protective agent comprises a cup body 1, a cup cover 2 is connected to one side of the cup body 1 through a thread, an extrusion structure 3 is connected to the cup cover 2, the extrusion structure 3 comprises an extrusion tube 31, a support rod 32, a discharge tube 34, a piston 36, a guide tube 37, a second spring 38, a sealing plug 39 and an outflow port 39a, the extrusion tube 31 penetrates through the cup cover 2, the extrusion tube 31 is fixedly connected to the cup cover 2, the support rod 32 is slidably connected to the inside of the extrusion tube 31, one end of the support rod 32 is fixedly connected to the piston 36, the cross section of the piston 36 is in an arc structure, the convex surface of the piston 36 is fixedly connected to one end of the support rod 32, the discharge tube 34 is fixedly connected to the cup cover 2, and one end of the discharge tube 34 is fixedly connected to the guide tube 37, one end of the guide pipe 37 is communicated with the discharge pipe 34, the other end of the guide pipe 37 is located at the bottom of the cup body 1, the other end of the discharge pipe 34 is connected with the sealing plug 39 in a sliding manner, one end of the sealing plug 39 is fixedly connected with the second spring 38, the other end of the second spring 38 is fixedly connected to the inner side wall of the discharge pipe 34, the outflow port 39a is further formed in the side face of one end of the discharge pipe 34, one side of the sealing plug 39 is connected with the discharge pipe 34 in a sliding manner through the outflow port 39a, and the middle of the cup cover 2 is provided with the stirring structure 4.

As a technical optimization scheme of the utility model, extrusion structure 3 still includes first spring 35, first spring 35 cup joint in on the bracing piece 32, first spring 35's one end with extrude pipe 31 fixed connection, first spring 35's the other end with bracing piece 32 fixed connection.

As a technical optimization scheme of the utility model, extrusion structure 3 still includes clamp plate 33, one side of clamp plate 33 with the one end of bracing piece 32 is rotated and is connected.

As a technical optimization scheme of the utility model, stirring structure 4 includes retainer plate 45, retainer plate 45 install in the inside wall of cup 1, pipe 37 with one side looks block of retainer plate 45.

As a technical optimization scheme of the utility model, stirring structure 4 still includes slide bar 41, hob 43 and stirring piece 44, slide bar 41 with 2 sliding connection of bowl cover, slide bar 41's one end with hob 43 fixed connection, slide bar 41's the other end with clamp plate 33 is inconsistent, hob 43 with the middle part threaded connection of stirring piece 44.

As a technical optimization scheme of the utility model, stirring structure 4 still includes sealing washer 42 and third spring 46, sealing washer 42 fixed connection in bowl cover 2, slide bar 41 with sealing washer 42 sliding connection, slide bar 41 has still cup jointed third spring 46, third spring 46's one end with bowl cover 2 fixed connection, third spring 46's the other end with slide bar 41 fixed connection.

As a technical optimization scheme of the utility model, stirring structure 4 still includes draw-in groove 47, one side of clamp plate 33 still is equipped with draw-in groove 47, draw-in groove 47 be with the same circular structure of slide bar 41 sectional area, just jaggedly is seted up to one side of draw-in groove 47, slide bar 41's one end is passed through draw-in groove 47 with clamp plate 33 looks block.

When the utility model is used, firstly, the proportioned cell freezing protection liquid is placed in the cup body 1, the cup cover 2 is rotated to enable the sealing between the cup cover 2 and the cup body 1 to be tight, when the cell freezing protection liquid needs to be taken, the upper pressing plate 33 of the cup cover 2 is extruded to enable the supporting rod 32 to slide the piston 36 downwards, due to the arc-shaped structure of the piston 36, the good sealing performance of the piston 36 is ensured in the moving process of the piston 36, along with the movement of the piston 36, the air pressure in the cup body 1 is gradually increased, the cell freezing protection liquid flows to the discharge pipe 34 through the guide pipe 37, along with the gradual increase of the pressure, the piston 36 finally positioned at the end part of the discharge pipe 34 is ejected, the side surface of the piston 36 is positioned on the discharge pipe 34 and is provided with the outflow port 39a, when the bottom end of the piston 36 slides to the position of the outflow, compared with a manual pouring mode, the extrusion amount is easier to control in the extrusion mode, meanwhile, when the pressing plate 33 presses to the bottommost part, a user releases the pressing plate 33, under the restoring action of the first spring 35, the supporting rod 32 and the piston 36 restore to the original position, and under the action of the second spring 38, the sealing plug 39 also restores to the original position to seal the discharge pipe 34, so that residual liquid is prevented from remaining at the pipe orifice, the solution is polluted when the pipe orifice is used next time, in the process of restoring the piston 36, because the air pressure in the cup body 1 is small, and the piston 36 is of an arc-shaped structure, the piston 36 deforms when sliding along the extrusion pipe 31, so that the edge part of the piston 36 bends towards the middle part, external air enters the cup body 1 through the extrusion pipe 31 and the edge of the piston 36, finally, the air pressure inside and outside the cup body 1 is balanced, and the pressing plate 33 can rotate in, make slide bar 41 tip block in draw-in groove 47, press the in-process that clamp plate 33 got the cell freezing protection liquid this moment, slide bar 41 can with bracing piece 32 synchronous motion for the stirring piece 44 that is located the installation on the inside retainer plate 45 of cup 1 rotates under the motion of hob 43, in order to stir inside solution, make the solution degree of mixing of extruding higher, when need not getting liquid in-process and stir, then only need rotate clamp plate 33, it can to remove clamp plate 33 and slide bar 41's block state.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. A storage device for cell cryoprotectants, comprising a cup body (1), characterized in that: one side of the cup body (1) is in threaded connection with the cup cover (2), the cup cover (2) is connected with an extrusion structure (3), the extrusion structure (3) comprises an extrusion pipe (31), a support rod (32), a discharge pipe (34), a piston (36), a guide pipe (37), a second spring (38), a sealing plug (39) and an outflow port (39a), the extrusion pipe (31) penetrates through the cup cover (2), the extrusion pipe (31) is fixedly connected with the cup cover (2), the support rod (32) is in sliding connection with the inside of the extrusion pipe (31), one end of the support rod (32) is fixedly connected with the piston (36), the cross section of the piston (36) is of an arc-shaped structure, the convex surface of the piston (36) is fixedly connected with one end of the support rod (32), the discharge pipe (34) is fixedly connected with the cup cover (2), one end fixedly connected with of discharge tube (34) pipe (37), the one end of pipe (37) with discharge tube (34) is linked together, the other end of pipe (37) is located the bottom of cup (1), the other end sliding connection of discharge tube (34) has sealing plug (39), the one end fixedly connected with of sealing plug (39) second spring (38), the other end fixed connection of second spring (38) in the inside wall of discharge tube (34), the one end side of discharge tube (34) has still been seted up egress opening (39a), one side of sealing plug (39) is passed through egress opening (39a) with discharge tube (34) sliding connection, the middle part of bowl cover (2) is equipped with stirring structure (4).

2. A cell cryoprotectant storage device according to claim 1, wherein: the extrusion structure (3) further comprises a first spring (35), the first spring (35) is sleeved on the supporting rod (32), one end of the first spring (35) is fixedly connected with the extrusion pipe (31), and the other end of the first spring (35) is fixedly connected with the supporting rod (32).

3. A cell cryoprotectant storage device according to claim 1, wherein: the extrusion structure (3) further comprises a pressing plate (33), and one side of the pressing plate (33) is rotatably connected with one end of the supporting rod (32).

4. A cell cryoprotectant storage device according to claim 1, wherein: stirring structure (4) include retainer plate (45), retainer plate (45) install in the inside wall of cup (1), pipe (37) with one side looks block of retainer plate (45).

5. A cell cryoprotectant storage device according to claim 3, wherein: stirring structure (4) still include slide bar (41), hob (43) and stirring piece (44), slide bar (41) with bowl cover (2) sliding connection, the one end of slide bar (41) with hob (43) fixed connection, the other end of slide bar (41) with clamp plate (33) are inconsistent, hob (43) with the middle part threaded connection of stirring piece (44).

6. A cell cryoprotectant storage device according to claim 5, wherein: stirring structure (4) still include sealing washer (42) and third spring (46), sealing washer (42) fixed connection in bowl cover (2), slide bar (41) with sealing washer (42) sliding connection, slide bar (41) still cup jointed third spring (46), the one end of third spring (46) with bowl cover (2) fixed connection, the other end of third spring (46) with slide bar (41) fixed connection.

7. A cell cryoprotectant storage device according to claim 5, wherein: the stirring structure (4) further comprises a clamping groove (47), the clamping groove (47) is further formed in one side of the pressing plate (33), the clamping groove (47) is of a circular structure with the same sectional area as that of the sliding rod (41), a notch is formed in one side of the clamping groove (47), and one end of the sliding rod (41) is clamped with the pressing plate (33) through the clamping groove (47).