CN211856485U - Chromatographic column with annular stirring paddle - Google Patents
Chromatographic column with annular stirring paddle Download PDFInfo
- Publication number
- CN211856485U CN211856485U CN201922476048.0U CN201922476048U CN211856485U CN 211856485 U CN211856485 U CN 211856485U CN 201922476048 U CN201922476048 U CN 201922476048U CN 211856485 U CN211856485 U CN 211856485U
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- chromatographic column
- stirring paddle
- column
- inlet
- stationary phase
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- 238000003756 stirring Methods 0.000 title claims abstract description 22
- 238000000926 separation method Methods 0.000 claims abstract description 14
- 239000000945 filler Substances 0.000 claims abstract description 8
- 238000012856 packing Methods 0.000 claims abstract description 8
- 229910003460 diamond Inorganic materials 0.000 claims abstract description 4
- 239000010432 diamond Substances 0.000 claims abstract description 4
- 230000005526 G1 to G0 transition Effects 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 4
- 238000002156 mixing Methods 0.000 abstract description 3
- 238000005191 phase separation Methods 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 12
- 239000000356 contaminant Substances 0.000 description 9
- 239000012491 analyte Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 150000007824 aliphatic compounds Chemical class 0.000 description 1
- 150000001491 aromatic compounds Chemical class 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 239000002285 corn oil Substances 0.000 description 1
- 235000005687 corn oil Nutrition 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004237 preparative chromatography Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
Images
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- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model relates to a chromatographic column with an annular stirring paddle, which comprises a chromatographic column and a separation plate, wherein the separation plate divides the chromatographic column into two parts, namely an upper chromatographic column and a lower chromatographic column; the upper end of the upper chromatographic column is provided with an inlet, the inlet is provided with a stirring rod, the lower end of the stirring rod is connected with a stirring paddle, and the packing of the upper chromatographic column is in a diamond shape; the filler of the lower chromatographic column is in a pentagram shape. The utility model discloses a mixing system can make sample and stationary phase fully contact, very big enrichment. The upper stationary phase can be replaced without affecting the effect of the lower stationary phase separation.
Description
[ technical field ] A method for producing a semiconductor device
The utility model belongs to the technical field of the chromatographic column technique and specifically relates to a chromatographic column with annular stirring rake.
[ background of the invention ]
Chromatography columns are the most critical components for performing chromatographic separation operations, determining the functionality of the constructed chromatographic system and the effectiveness of the developed separation methods. The research on chromatographic column type and chromatographic packing is always a hot problem in the technical field of chromatography. In recent years, with the rapid development of life sciences and pharmaceutical industries, novel preparative chromatography techniques have also been developed rapidly.
In actual research, sample matrixes such as corn oil, higher aromatic compounds and wax exist, adhere to the surface of the packing material and change the properties of the packing material, biological samples containing protein components are adsorbed on the surface of the packing material particles, and even if a sample pretreatment method is adopted to prevent foreign substances from being polluted to protect a chromatographic column, the matrixes of the substances to be tested are adsorbed on the surface of the chromatographic column finally. The chromatographic behavior of a column after being contaminated will be different from that of an uncontaminated column. Typically, the sample matrix will contain some contaminants that are not associated with the target analyte. Such as salts, esters, aliphatic compounds, organic acids, hydrophobic proteins and other biological components, which are substances that may interact with the stationary phase. The retention capacity of these contaminants may be greater than the target analyte. Once these unexpected contaminants are detected by the detector, they appear as a bifurcation of the chromatographic peak on the chromatogram, with baseline fluctuations and even negative peaks. If the contaminants are retained on the column sufficiently and the mobile phase is not sufficiently mobile to flush out the contaminants, the contaminants that have adsorbed on the column accumulate after multiple injections. When the contaminants accumulate sufficiently, they behave like modified fillers and the interaction of the target analyte with these contaminants affects their original separation mechanism, resulting in retention time fluctuations and peak tailing. If the contaminants are more, the column pressure may rise to a very high level, even beyond the upper column pressure limit. An object of the utility model is to overcome prior art not enough, provide a chromatographic column with annular stirring rake, separation these pollutants of ability furthest makes it not enter the interior separation effect that influences the post of post.
[ Utility model ] content
The purpose of the utility model is realized through the following technical scheme:
a chromatographic column with an annular stirring paddle comprises a chromatographic column and a separation plate, wherein the separation plate divides the chromatographic column into two parts, namely an upper chromatographic column and a lower chromatographic column; the upper end of the upper chromatographic column is provided with an inlet, the inlet is provided with a stirring rod, the lower end of the stirring rod is connected with a stirring paddle, and the packing of the upper chromatographic column is in a diamond shape; the filler of the lower chromatographic column is in a pentagram shape.
The separating plate is uniformly provided with round and triangular holes.
An outlet is provided at the lower end of the column and the sample is connected to the sample reservoir by a conduit.
Compared with the prior art, the utility model has the positive effects that:
(1) the chromatographic column designed by the utility model is equivalent to the improved FLASH chromatograph; the upper filler has the function of sample mixing; the effects of continuous enrichment and sample introduction and separation are achieved;
(2) the utility model discloses a mixing system can make sample and stationary phase fully contact, very big enrichment. The upper stationary phase can be replaced, and the effect of the lower stationary phase separation is not influenced;
(3) the utility model has the advantages of simple preparation process and low cost.
[ description of the drawings ]
FIG. 1 is a schematic structural diagram of the present application;
the reference numbers in the figures are respectively:
the chromatographic column comprises an upper chromatographic column 1, a filler of an upper chromatographic column 2, a separation plate 3, a lower chromatographic column 4, a filler of a lower chromatographic column 5, a stirring rod 6, a liquid inlet pipe 7, a stirring paddle 8, a circular hole 9, a triangular hole 10, an outlet 11 and a sample groove 12.
[ detailed description ] embodiments
The following provides a specific embodiment of the utility model for preparing a special chromatographic column for trace components with large sample loading amount.
Example 1
A chromatographic column with an annular stirring paddle comprises a chromatographic column and a separation plate, wherein the separation plate 3 divides the chromatographic column into two parts, namely an upper chromatographic column 1 and a lower chromatographic column 4; the upper end of the upper chromatographic column is provided with an inlet, the inlet is provided with a stirring rod 6, the lower end of the stirring rod is connected with a stirring paddle 8, and the filler 2 of the upper chromatographic column is in a diamond shape; the packing 5 of the lower chromatographic column is in the shape of a pentagram.
The inlet of the upper chromatographic column is also provided with a liquid inlet pipe 7.
The separating plate is uniformly provided with a circle 9 and a triangular hole 10.
At the lower end of the column an outlet 11 is provided through which the sample is connected to a sample holder 12.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of improvements and decorations can be made without departing from the concept of the present invention, and these improvements and decorations should also be considered as within the protection scope of the present invention.
Claims (1)
1. A chromatographic column with an annular stirring paddle is characterized by comprising a chromatographic column and a separation plate, wherein the separation plate divides the chromatographic column into two parts, namely an upper chromatographic column and a lower chromatographic column; the upper end of the upper chromatographic column is provided with an inlet, the inlet is provided with a stirring rod, the lower end of the stirring rod is connected with a stirring paddle, and the packing of the upper chromatographic column is in a diamond shape; the filler of the lower chromatographic column is in a pentagram shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922476048.0U CN211856485U (en) | 2019-12-31 | 2019-12-31 | Chromatographic column with annular stirring paddle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922476048.0U CN211856485U (en) | 2019-12-31 | 2019-12-31 | Chromatographic column with annular stirring paddle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211856485U true CN211856485U (en) | 2020-11-03 |
Family
ID=73209944
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922476048.0U Active CN211856485U (en) | 2019-12-31 | 2019-12-31 | Chromatographic column with annular stirring paddle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211856485U (en) |
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2019
- 2019-12-31 CN CN201922476048.0U patent/CN211856485U/en active Active
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