CN211847977U - Automatic constant-temperature water replenishing device used in stem cell culture process - Google Patents
Automatic constant-temperature water replenishing device used in stem cell culture process Download PDFInfo
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- CN211847977U CN211847977U CN201922024810.1U CN201922024810U CN211847977U CN 211847977 U CN211847977 U CN 211847977U CN 201922024810 U CN201922024810 U CN 201922024810U CN 211847977 U CN211847977 U CN 211847977U
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Abstract
The utility model discloses an automatic constant temperature moisturizing device for stem cell culture in-process, including water bath, water charging system, water level detection device and controller. In the water replenishing system, water in a hot water pipeline is heated by a heating part and flows into an automatic thermostatic valve together with cold water in a cold water pipeline; the automatic thermostatic valve ensures that the temperature of the water flowing out is at the set temperature. The water flowing out of the automatic thermostatic valve flows into the converging pipeline and then enters the automatic backflow three-way valve, and two output ends of the automatic backflow three-way valve are respectively communicated with the output pipeline and the backflow pipeline. The position of the converging pipeline close to the output end is provided with a temperature probe which is used for detecting whether the water temperature at the output end of the converging pipeline is at a set temperature or not, if so, the water flows into the output pipeline, and otherwise, the water flows into the return pipeline and reenters the hot water pipeline. The water in the output pipe finally flows into the water bath. The water level detection device in the water bath kettle is matched with the electromagnetic valve on the output pipeline to control whether the water bath kettle supplements water or not. The controller is the utility model discloses a control center.
Description
Technical Field
The utility model belongs to the laboratory glassware field especially relates to an automatic constant temperature moisturizing device for stem cell culture in-process.
Background
In the general stem cell culture process, reagents, culture solution, digestive enzymes and the like need to be preheated at a certain constant temperature to ensure the stability of the experiment and the cell culture experiment, and a common heating device is a water bath kettle.
The existing water bath is generally used after adding a proper amount of water before use and then heating to a preset temperature. However, when the constant temperature heating time is long or continuous use is required, the water in the water bath kettle is gradually reduced due to evaporation, so that the water level is reduced, the experimental result is influenced, and water needs to be supplemented in time.
The existing water bath kettle can automatically supplement water at constant temperature, and the water to be heated can directly flow into the water bath kettle only by heating the water to be heated to a specified temperature at constant temperature. However, the water flowing from the heating point to the water bath may pass through a pipe at one end, where the water may dissipate heat and may not be at the set temperature when entering the water bath.
Disclosure of Invention
In order to solve the problems, the to-be-solved technical problem of the utility model is to provide an automatic constant temperature moisturizing device for stem cell culture in-process for solve the current constant temperature problem that the temperature fluctuation is big that adds water of water device.
The technical scheme of the utility model is that:
an automatic constant-temperature water supplementing device used in a stem cell culture process is characterized by comprising a water bath, a water supplementing system, a water level detection device and a controller;
at least one water inlet and a corresponding flow dividing pipeline are arranged on the periphery of the water bath kettle, the setting height of the water inlet is lower than the lowest limit water level height of the water bath kettle, and each water inlet is communicated with the output end of the corresponding flow dividing pipeline; the water level detection device is arranged in the water bath;
the water charging system includes cold water pipeline, hot water pipeline, joins pipeline, backflow pipeline and output pipeline, still includes:
the first input end of the automatic thermostatic valve is communicated with the output end of the cold water pipeline, the second input end of the automatic thermostatic valve is communicated with the output end of the hot water pipeline, and the output end of the automatic thermostatic valve is communicated with the input end of the converging pipeline; the input end of the cold water pipeline and the input end of the hot water pipeline are both communicated with an external water pipe, a heating section is arranged on the hot water pipeline, and a heating part is arranged on the heating section;
an input end of the automatic backflow three-way valve is communicated with an output end of the confluence pipeline, and a first output end of the automatic backflow three-way valve is communicated with an input end of the output pipeline; the second output end of the automatic backflow three-way valve is communicated with the input end of the backflow pipeline, and the output end of the backflow pipeline is communicated with the hot water pipeline; a temperature probe is arranged on the output end of the converging pipeline;
the output end of the output pipeline is a total water inlet, and the input ends of all the shunt pipelines are communicated with the total water inlet; an electromagnetic valve is arranged on the output pipeline close to the automatic backflow three-way valve;
the water level detection device, the heating part, the temperature probe, the automatic backflow three-way valve and the electromagnetic valve are respectively in electric signal connection with the controller;
when the water level detection device detects that the water level in the water bath is lower than a set lowest water level, the controller controls the electromagnetic valve to be opened, and after the water level detection device detects that the water level in the water bath is higher than a set highest water level, the controller controls the electromagnetic valve to be closed;
when the temperature probe detects that the temperature of joining the pipeline accords with the settlement temperature, the controller control the first output of automatic backward flow three-way valve is opened and the second output is closed, works as the temperature probe detects the temperature deviation of joining the pipeline is when setting for the temperature, the controller control the first output of automatic backward flow three-way valve is closed and the second output is opened.
In an embodiment of the present invention, the heating section is a spiral pipe, and the heating portion is attached to the spiral pipe.
The utility model discloses an in the embodiment, be equipped with temperature controller and flow controller on the automatic thermostatic valve, temperature controller is used for setting for and control the temperature of automatic thermostatic valve output rivers, flow controller is used for control the size of automatic thermostatic valve output rivers.
The utility model discloses an embodiment, be equipped with one-way stagnant water valve on the backflow pipeline.
The utility model discloses an in the embodiment, each all be equipped with one-way stagnant water valve on the reposition of redundant personnel pipeline.
The utility model discloses an in the embodiment, the output of cold water pipeline is equipped with first filter screen, hot water pipeline's output is equipped with the second filter screen.
In an embodiment of the present invention, each of the water inlets is provided with a partition plate, and the partition plate is provided with a plurality of through holes.
In an embodiment of the present invention, the number of the water inlets is four or six, and the water inlets are uniformly distributed around the water bath.
In an embodiment of the present invention, the total water inlet is higher than the maximum limit water level of the water bath.
In an embodiment of the present invention, the device further comprises a temperature detection device, an alarm indicator light and a communication module; the temperature detection device is arranged in the water bath kettle, and the setting height of the temperature detection device is lower than the lowest limit water level height of the water bath kettle; the temperature detection device, the alarm indicator light and the communication module are respectively in electric signal connection with the controller.
The utility model discloses owing to adopt above technical scheme, make it compare with prior art and have following advantage and positive effect:
(1) the water in the hot water pipeline is heated by the heating part to become hot water, and the cold water in the cold water pipeline respectively flows into the automatic thermostatic valve, the automatic thermostatic valve is provided with a specified temperature range, and when the mixed temperature of the input hot water and the input cold water reaches the specified temperature range, the mixed water flows into the converging pipeline. In order to prevent the water temperature from changing in the converging pipeline and exceeding a specified temperature range, a temperature probe is arranged at the output end of the converging pipeline, when the temperature probe detects that the water temperature in the converging pipeline is in the specified temperature range, the automatic backflow three-way valve opens a switch leading to the output pipeline and closes the switch leading to the backflow pipeline, and constant-temperature water is output to the output pipeline; when the water temperature deviates from the designated temperature range, the automatic backflow three-way valve opens the switch leading to the backflow pipeline and closes the switch leading to the output pipeline, so that the water with the temperature not reaching the designated temperature range flows back to the hot water pipeline through the backflow pipeline and flows through the thermostatic valve again. Wherein, the water reaching the designated temperature passes through the output pipeline and enters the water bath from the water inlet through the shunt pipeline from the total water inlet of the output pipeline. Make the embodiment of the utility model discloses a have the function of constant temperature moisturizing, supply the temperature of the water in the water bath from the external world promptly always for setting for the temperature, realized the effect of the temperature of the constant temperature water that the accurate control added, solved the current constant temperature and added the undulant big problem of temperature of water installation.
(2) A water level detection device is arranged in the water bath, when the water level in the water bath is lower than the lowest limit water level, the electromagnetic valve is opened, and the water supplementing system supplements water to the water bath. And when the water level in the water bath is higher than the highest limit water level, the electromagnetic valve is closed, and the water supplementing system stops supplementing water to the water bath. Make the embodiment of the utility model provides an automatic water supply's function has been realized.
(3) The water inlet of the water bath is arranged at a height lower than the minimum limit water level of the water bath, which is a gentle means for mixing the supplemented water with the water in the water bath. If the set height of the water gap is equal to or higher than the minimum limit water level height of the water bath kettle, the supplemented water can be mixed with the water in the water bath kettle in a waterfall or rain-like mode, so that originally calm water in the water bath kettle vibrates and oscillates, and the water environment in the water bath kettle is damaged. Meanwhile, the height of the water inlet of the water bath is lower than the minimum limit water level of the water bath, so that the water temperature is reduced under the influence of the external environment during water replenishing, and constant temperature is ensured.
Drawings
The following detailed description of embodiments of the present invention is provided with reference to the accompanying drawings, in which:
FIG. 1 is a schematic structural view of an automatic water supply device for stem cell culture according to the present invention;
FIG. 2 is a schematic view of a water inlet partition of an automatic water replenishing apparatus for a stem cell culturing process;
FIG. 3 is a schematic view of an operation display platform of an automatic water supply device for stem cell culture process.
Description of reference numerals:
1: a water bath kettle; 2: a diversion pipeline; 3: a cold water pipe; 4: a hot water pipe; 5: a converging duct; 6: a return line; 7: an output pipe; 8: an automatic thermostatic valve; 9: a heating section; 10: an automatic backflow three-way valve; 11: a temperature probe; 12: a total water inlet; 13: an electromagnetic valve; 14: a one-way water stop valve; 15: outputting a temperature display screen; 16: a temperature adjusting knob; 17: a flow adjustment knob; 18: a water inlet; 19: a partition plate; 20: a through hole; 21: operating the display platform; 22: operation of the input port: 23: a water level display; 24: an actual temperature display; 25: setting a temperature display; 26: and an alarm indicator light.
Detailed Description
The following will explain in detail an automatic constant temperature water replenishing device for stem cell culture process according to the present invention with reference to the accompanying drawings and specific embodiments. The advantages and features of the present invention will become more fully apparent from the following description and appended claims. It is to be noted that the drawings are in a very simplified form and are not to be construed as precise ratios as are merely intended to facilitate and distinctly illustrate the embodiments of the present invention.
Referring to fig. 1, in one embodiment, an automatic constant temperature water supplement apparatus for use in a stem cell culture process includes a water bath 1, a water supplement system, a water level detection device, and a controller. At least one water inlet 18 and the corresponding flow dividing pipeline 2 are arranged on the periphery of the water bath pot 1, the setting height of the water inlet 18 is lower than the lowest limit water level height of the water bath pot 1, and each water inlet 18 is communicated with the output end of the corresponding flow dividing pipeline 2; the water level detection device is arranged in the water bath kettle 1. The water supplementing system comprises a cold water pipeline 3, a hot water pipeline 4, a converging pipeline 5, a return pipeline 6 and an output pipeline 7, and further comprises an automatic thermostatic valve 8, an automatic return three-way valve 10 and an electromagnetic valve 13. The first input end of the automatic thermostatic valve 8 is communicated with the output end of the cold water pipeline 3, the second input end of the automatic thermostatic valve 8 is communicated with the output end of the hot water pipeline 4, and the output end of the automatic thermostatic valve 8 is communicated with the input end of the converging pipeline 5. The input of cold water pipeline 3 and the input of hot water pipeline 4 all communicate with outside water pipe, are equipped with heating section 9 on the hot water pipeline 4, are equipped with heating portion on the heating section 9. And an input end of the automatic backflow three-way valve 10 is communicated with an output end of the converging pipeline 5, and a first output end of the automatic backflow three-way valve 10 is communicated with an input end of the output pipeline 7. The second output end of the automatic backflow three-way valve 10 is communicated with the input end of the backflow pipeline 6, and the output end of the backflow pipeline 6 is communicated with the hot water pipeline 4. The output end of the converging pipeline 5 is provided with a temperature probe 11. The output end of the output pipeline 7 is a total water inlet 12, and the input ends of the shunt pipelines 2 are communicated with the total water inlet 12. An electromagnetic valve 13 is arranged on the output pipeline 7 and close to the automatic backflow three-way valve 10. The water level detection device, the heating part, the temperature probe 11, the automatic backflow three-way valve 10 and the electromagnetic valve 13 are respectively connected with the controller through electric signals. When the water level detection device detects that the water level in the water bath kettle 1 is lower than the set lowest water level, the controller controls the electromagnetic valve 13 to be opened, and when the water level detection device detects that the water level in the water bath kettle 1 is higher than the set highest water level, the controller controls the electromagnetic valve 13 to be closed. When the temperature probe 11 detects that the temperature of the converging pipeline 5 accords with the set temperature, the controller controls the first output end of the automatic backflow three-way valve 10 to be opened and the second output end to be closed, and when the temperature probe 11 detects that the temperature of the converging pipeline 5 deviates from the set temperature, the controller controls the first output end of the automatic backflow three-way valve 10 to be closed and the second output end to be opened.
In the present embodiment, the water in the hot water pipe 4 is changed into hot water by heating of the heating part, and the cold water in the cold water pipe 3 is respectively flowed into the automatic thermostatic valve 8, the automatic thermostatic valve 8 is provided with a designated temperature range, and when the temperature of the inputted hot water and cold water mixed reaches the designated temperature range, it is flowed into the merging pipe 5. In order to prevent the water temperature from changing in the merging pipe 5 beyond a specified temperature range, a temperature probe 11 is arranged at the output end of the merging pipe 5, when the temperature probe 11 detects that the water temperature in the merging pipe 5 is within the specified temperature range, an automatic backflow three-way valve 10 opens a switch leading to the output pipe 7 and closes a switch leading to the backflow pipe 6, and constant-temperature water is output to the output pipe 7; when the temperature of the water deviates from the specified temperature range, the automatic return three-way valve 10 opens the switch to the return pipe 6 and closes the switch to the output pipe 7, so that the water having a temperature not reaching the specified temperature range flows back into the hot water pipe 4 through the return pipe 6 and flows through the thermostatic valve again. Wherein water reaching a prescribed temperature passes through the output pipe 7, enters the water bath 1 from the water inlet 18 through the branch pipe 2 from the total water inlet 12 of the output pipe 7. Make the embodiment of the utility model discloses a have the function of constant temperature moisturizing, supply the temperature of the water in the water bath 1 from the external world promptly for setting for the temperature always, realized the effect of the temperature of the constant temperature water that the accurate control added, solved the current constant temperature and added the undulant big problem of temperature of water installation.
This embodiment can also automatic water supply, has water level detection device in the water bath 1, and when the water level in the water bath 1 was less than minimum restriction water level height, open solenoid valve 13, water charging system carries out the moisturizing to water bath 1. When the water level in the water bath 1 is higher than the highest limit water level, the electromagnetic valve 13 is closed, and the water supplementing system stops supplementing water to the water bath 1. Wherein, the lowest limit water level height and the lowest limit water level height are determined according to the water level height required in the experiment.
In the embodiment, the water inlet 18 of the water bath 1 is arranged at a height lower than the minimum limit water level of the water bath 1, which is a gentle means for mixing the supplementary water with the water in the water bath 1. If the setting height of the water gap is equal to or higher than the lowest limit water level height of the water bath kettle 1, the supplemented water can be mixed with the water in the water bath kettle 1 in a waterfall or rain-like mode, so that the originally calm water in the water bath kettle 1 vibrates and vibrates, and the water environment of stem cell culture in the water bath kettle 1 is damaged. Meanwhile, the height of the water inlet 18 of the water bath 1 is lower than the minimum limit water level of the water bath 1, so that the water temperature is reduced under the influence of the external environment during water replenishing, and the constant temperature is ensured.
This example is used for stem cell culture experiments and is a heating device in the experiments. According to the characteristics of the stem cells, the temperature of the heating device needs to be set to a temperature suitable for the survival of the stem cells, namely 37 ℃. In this embodiment, the heating device is the water bath 1, and simultaneously, this embodiment still has moisturizing system and some control detection device, ensures that this embodiment can realize can automatic detection and add the water that accords with the temperature setting when water bath 1 lacks water. Therefore, the temperature of the water bath 1 is 37 degrees, and the temperature of the water added from the outside is also 37 degrees.
In this embodiment, the water level detecting device sends a signal indicating that the water level in the water bath 1 is detected to the controller, and the controller determines that the water level in the water bath 1 is lower than the minimum limit water level, and sends a signal to the electromagnetic valve 13 and the heating part, so that the electromagnetic valve 13 is opened and the heating part starts heating the heating section 9. Then, if the controller receives the water level signal and judges that the water level in the water bath 1 reaches the highest limit water level gauge, the controller sends a signal to the electromagnetic valve 13 and the heating part, the electromagnetic valve 13 is closed, and the heating part stops heating. In this process, the water level detecting means may be an infrared sensing means. The low water level infrared sensing device is arranged at the lowest limit water level in the water bath 1, and the high water level infrared sensing device is arranged at the highest limit water level in the water bath 1. When the water in the water bath 1 does not overflow the low-water-level infrared sensing device any more, the low-water-level infrared sensing device sends a signal to the controller, and a subsequent water supplementing system such as an electromagnetic valve 13 is started to supplement constant-temperature water to the water bath 1; when the water level in the water bath 1 overflows the high-water-level infrared sensing device in the water replenishing process, the high-water-level infrared sensing device sends a signal to the controller, and the electromagnetic valve 13 and other water replenishing systems are closed.
In the present embodiment, the specified temperatures of the automatic thermostat valve 8 and the temperature probe 11 are both 37 degrees. The automatic thermostat valve 8 controls the temperature of water flowing out of the automatic thermostat valve 8 to 37 degrees. The temperature probe 11 detects whether the water temperature in the converging pipeline 5 is kept at 37 ℃, if so, the automatic return valve opens the first input end to close the second input end, and constant-temperature water flows into the output pipeline 7; if not, the automatic thermostatic valve 8 opens the second input end and closes the first input end, and water with the water temperature not reaching the standard flows into the return pipeline 6 and then returns to the hot water pipeline 4 again to flow into the automatic thermostatic valve 8.
Further, in the embodiment of the present invention, there is also provided an operation input port 22, which can be used to set various data after cooperating with the controller, such as the setting of the temperature in the water bath 1, the temperature setting selected by the two output terminals of the temperature probe 11 connected to the automatic backflow three-way valve 10, the setting of the minimum limit water level and the maximum limit water level for determining the switch solenoid valve 13, and the like.
Further, in the embodiment of the present invention, the electromagnetic valve 13 may also be an electromagnetic valve 13 having a flow control function. The speed of the water flow is controlled during the opening of the solenoid valve 13 for water replenishment. Although the temperature of the water replenishing water is set to be 37 degrees, and the temperature in the water bath kettle 1 is also set to be 37 degrees, a certain temperature difference still exists between the water replenishing water and the water bath kettle. The requirement of the stem cell culture environment on the temperature is very strict, and if a large amount of supplementing water enters at one time, the water temperature in the water bath 1 is necessarily changed greatly, and the large water temperature change is of course relative to the stem cell culture experiment. Therefore, the electromagnetic valve 13 with the flow control function has unique benefits when used in stem cell culture experiments.
Further, in the embodiment of the present invention, the heating section 9 on the hot water pipeline 4 is set to be a spiral pipeline, and the heating sheet is attached to the outside of the spiral pipeline. The setting of helical tube can make heating tube have heating section 9 as far as possible in limited laboratory, and the laminating sets up the water in the heating plate ability faster heating helical tube of helical tube outside the helical tube, and heating efficiency is higher. In other specific embodiments, the heating portion may be a heating device such as a heating wire disposed in the heating section 9.
Furthermore, in the embodiment of the present invention, the automatic thermostatic valve 8 has a temperature adjusting knob 16, a set output temperature display screen 15 and a flow rate adjusting knob 17. The output end of the automatic thermostatic valve 8 is provided with a thermosensitive element, a special wax/copper mixture is used as a temperature sensing medium, the thermosensitive element is arranged at the mixing position of hot water and cold water in the automatic thermostatic valve 8 to sense the temperature of the mixed water, and the mixing ratio of the hot water and the cold water is automatically adjusted by driving a valve rod according to the temperature set value on the valve. After the temperature adjusting knob 16 sets a certain temperature, the ratio of the cold water and the hot water entering the water outlet changes with the change of the inlet temperature and the pressure of the cold water and the hot water, so that the temperature of the water flowing out of the output end is always constant, namely the temperature of the water flowing into the converging pipeline 5 is controlled. The above-mentioned internal arrangement of the automatic thermostat valve 8 corresponds to a temperature control device. In other embodiments, the temperature control device may be other devices that can ensure that the water temperature at the output of the thermostat 8 is at the set value. In the present embodiment, the output water temperature of the automatic thermostatic valve 8 can be set to 37 degrees for the culture experiment of stem cells. And a liquid flow meter is arranged at the output end of the automatic thermostatic valve 8 and used for controlling the water flow speed flowing out of the automatic thermostatic valve 8 and further controlling the water flow speed in the whole water replenishing system. In other embodiments, the flow controller may be other devices that achieve a flow rate at the output of the thermostat valve 8. The specific flow value of the flow controller is set by the flow adjustment knob 17. The temperature adjusting knob 16 and the flow rate adjusting knob 17 are shaped to conform to the shape grasped by a human hand.
Further, the embodiment of the utility model provides an in, can set up one-way check valve 14 in backflow pipeline 6, prevent that the phenomenon of rivers backward flow in backflow pipeline 6 from appearing in the water charging system. A one-way water stop valve 14 may also be provided in each of the diversion pipes 2 to prevent water in the water bath 1 from flowing back into the water replenishment system.
Further, in the embodiment of the utility model provides an in, the output of cold water pipeline 3 and the output of hot water pipeline 4 all can set up the filter screen for impurity in the external rivers of filtration, incrustation scale etc. prevent that it from getting into 1 cultivation environment that pollutes the stem cell of water bath. The output end of the cold water pipeline 3 and the output end of the hot water pipeline 4 can be detached from the two input ends of the automatic thermostatic valve 8, and then the filter screen in the automatic thermostatic valve is taken out, so that the filter screen is detached and replaced. In other embodiments, the filter screen may be installed at the input end of the cold water pipe 3 and the input end of the hot water pipe 4, and the input end of the cold water pipe 3 and the input end of the hot water pipe 4 may be conveniently detached when being connected to an external water pipe, so as to facilitate replacement of the filter screen.
Referring to fig. 2, further, in the embodiment of the present invention, a plurality of water inlets 18, for example, four or six, may be disposed on the water bath 1, and these water inlets 18 are uniformly distributed around the water bath 1. All water inlets 18 are provided with partition plates 19, the partition plates 19 are provided with a plurality of through holes 20, and the plurality of through holes 20 can enable the constant-temperature water added into the water bath kettle 1 to be output in a plurality of water flows, so that the constant-temperature water can be mixed with the water in the water bath kettle 1 more quickly. The plurality of water inlets 18 and the water inlets 18 are provided with perforated baffles 19 to provide more rapid mixing of the replenished water with the water in the water bath 1.
Further, in the embodiment of the present invention, the total water inlet 12 is disposed at a height higher than the maximum limit water level. This is advantageous in preventing the water in the water bath 1 from flowing back into the water replenishing system.
In the whole water replenishing system, when the water bath 1 does not need to be replenished, the pipeline between the electromagnetic valve 13 and the automatic return three-way valve 10 becomes a closed space. When the water bath kettle 1 does not need to be supplemented with water, the water temperature in the pipeline can be reduced, and the problem that the water in the pipeline cannot reach the specified temperature possibly occurs when the water is required to be supplemented next time. Aiming at the problem, a drain valve can be arranged on the section of pipeline, and before water is supplemented each time, the controller sends a signal to the drain valve to enable the drain valve to work to drain the section of water.
Further, the embodiment of the utility model provides a still include temperature-detecting device, alarm indicator 26 and communication module. The temperature detection device is arranged in the water bath kettle 1, and the set height is lower than the lowest limit water level height of the water bath kettle 1. The temperature detection device is used for detecting the water temperature in the water bath 1. The temperature detection device, the alarm indicator lamp 26 and the communication module are respectively connected with the controller through electric signals. The alarm indicator lamp 26 and the communication module are triggered if the water bath 1 has a system failure or other abnormal conditions. The alarm indicator lamp 26 is used for reminding people in the laboratory that the water bath 1 has problems, and the communication module is used for sending information to related people. System faults or abnormal situations are, for example: the water bath kettle 1 is provided with a safe water level range and a safe water temperature range, and when the water level detection device detects that the water level in the water bath kettle 1 is not in the safe water level range, the water level detection device triggers the alarm indicator lamp 26 and the communication module to send out alarm prompt and inform related personnel; when the temperature detection device detects that the water temperature in the water bath 1 is not in the safe water temperature range, the alarm indicator lamp 26 and the communication module are triggered to give an alarm prompt and inform related personnel; the water bath 1 is provided with an emergency power supply which is started and triggers the alarm indicator lamp 26 and the communication module to give an alarm prompt and inform related personnel and the like once the conventional power supply is powered off. The operator input port 22 may be used to set a safe water level range and a safe water temperature range. The alarm indicator lamp 26, the communication module and the corresponding supporting facilities are arranged to facilitate timely finding and quickly solving problems after the water bath 1 has problems.
Referring to fig. 3, further, the embodiment of the present invention further includes an operation display platform 21, and the operation input port 22 and the alarm indicator 26 are located on the operation display platform 21. A water level display 23, an actual temperature display 24, a set temperature display 25, and the like may be further provided on the operation display platform 21. The water level detection device detects the actual water level height in the water bath 1 and sends a signal to the controller, and the controller controls the water level display 23 to display the actual water level numerical value. If a device such as an infrared sensor device which cannot detect a specific water level is used as the water level detecting device, a water level detecting device which can detect a specific water level needs to be installed in the water bath 1 if the water level display 23 is required.
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments. Even if various changes are made to the present invention, the changes are still within the scope of the present invention if they fall within the scope of the claims and their equivalents.
Claims (10)
1. An automatic constant-temperature water supplementing device used in a stem cell culture process is characterized by comprising a water bath, a water supplementing system, a water level detection device and a controller;
at least one water inlet and a corresponding flow dividing pipeline are arranged on the periphery of the water bath kettle, the setting height of the water inlet is lower than the lowest limit water level height of the water bath kettle, and each water inlet is communicated with the output end of the corresponding flow dividing pipeline; the water level detection device is arranged in the water bath;
the water charging system includes cold water pipeline, hot water pipeline, joins pipeline, backflow pipeline and output pipeline, still includes:
the first input end of the automatic thermostatic valve is communicated with the output end of the cold water pipeline, the second input end of the automatic thermostatic valve is communicated with the output end of the hot water pipeline, and the output end of the automatic thermostatic valve is communicated with the input end of the converging pipeline; the input end of the cold water pipeline and the input end of the hot water pipeline are both communicated with an external water pipe, a heating section is arranged on the hot water pipeline, and a heating part is arranged on the heating section;
an input end of the automatic backflow three-way valve is communicated with an output end of the confluence pipeline, and a first output end of the automatic backflow three-way valve is communicated with an input end of the output pipeline; the second output end of the automatic backflow three-way valve is communicated with the input end of the backflow pipeline, and the output end of the backflow pipeline is communicated with the hot water pipeline; a temperature probe is arranged on the output end of the converging pipeline;
the output end of the output pipeline is a total water inlet, and the input ends of all the shunt pipelines are communicated with the total water inlet; an electromagnetic valve is arranged on the output pipeline close to the automatic backflow three-way valve;
the water level detection device, the heating part, the temperature probe, the automatic backflow three-way valve and the electromagnetic valve are respectively in electric signal connection with the controller;
when the water level detection device detects that the water level in the water bath is lower than a set lowest water level, the controller controls the electromagnetic valve to be opened, and after the water level detection device detects that the water level in the water bath is higher than a set highest water level, the controller controls the electromagnetic valve to be closed;
when the temperature probe detects that the temperature of joining the pipeline accords with the settlement temperature, the controller control the first output of automatic backward flow three-way valve is opened and the second output is closed, works as the temperature probe detects the temperature deviation of joining the pipeline is when setting for the temperature, the controller control the first output of automatic backward flow three-way valve is closed and the second output is opened.
2. The automatic constant-temperature water replenishing device for the stem cell culture process according to claim 1, wherein the heating section is a spiral pipeline, and the heating part is attached to the spiral pipeline.
3. The automatic constant temperature water replenishing device for the stem cell culture process according to claim 1, wherein a temperature controller and a flow controller are arranged on the automatic constant temperature valve, the temperature controller is used for setting and controlling the temperature of the water flow output by the output end of the automatic constant temperature valve, and the flow controller is used for controlling the size of the water flow output by the output end of the automatic constant temperature valve.
4. The automatic constant-temperature water replenishing device for the stem cell culture process according to claim 1, wherein a one-way water stop valve is arranged on the return pipeline.
5. The automatic constant-temperature water replenishing device for the stem cell culture process according to claim 1, wherein each of the diversion pipelines is provided with a one-way water stop valve.
6. The automatic constant-temperature water replenishing device for the stem cell culture process according to claim 1, wherein a first filter screen is arranged at the output end of the cold water pipeline, and a second filter screen is arranged at the output end of the hot water pipeline.
7. The automatic constant temperature water replenishing device for stem cell culture process according to claim 1, wherein each water inlet is provided with a partition plate, and the partition plate is provided with a plurality of through holes.
8. The automatic constant-temperature water replenishing device for the stem cell culture process according to claim 1, wherein the number of the water inlets is four or six, and the water inlets are uniformly distributed on the peripheral side of the water bath.
9. The automatic thermostatic water replenishing device for stem cell culture process according to claim 1, wherein the total water inlet is set at a height higher than the highest limit water level of the water bath.
10. The automatic constant-temperature water replenishing device for the stem cell culture process according to claim 1, further comprising a temperature detection device, an alarm indicator light and a communication module; the temperature detection device is arranged in the water bath kettle, and the setting height of the temperature detection device is lower than the lowest limit water level height of the water bath kettle; the temperature detection device, the alarm indicator light and the communication module are respectively in electric signal connection with the controller.
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| CN201922024810.1U CN211847977U (en) | 2019-11-21 | 2019-11-21 | Automatic constant-temperature water replenishing device used in stem cell culture process |
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| CN201922024810.1U CN211847977U (en) | 2019-11-21 | 2019-11-21 | Automatic constant-temperature water replenishing device used in stem cell culture process |
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