CN211847968U - Temperature and humidity adjustable biological cell culture device - Google Patents
Temperature and humidity adjustable biological cell culture device Download PDFInfo
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- CN211847968U CN211847968U CN202020326596.5U CN202020326596U CN211847968U CN 211847968 U CN211847968 U CN 211847968U CN 202020326596 U CN202020326596 U CN 202020326596U CN 211847968 U CN211847968 U CN 211847968U
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Abstract
The utility model relates to a temperature and humidity adjustable biological cell culture device, include: the box body comprises a first chamber, a second chamber, a third chamber, at least one circulating pipe, at least one humidifying pipe, a heating plate, a connecting pipe, an exhaust device, a humidity detection element and a temperature detection element. The box body has the advantages that the interior of the box body is sequentially provided with the first chamber, the second chamber and the third chamber from top to bottom, the third chamber heats the second chamber, the first chamber is subjected to indirect temperature regulation and direct humidity regulation by utilizing the evaporation effect in the second chamber, and the uniformity of the regulation of the temperature and the humidity is ensured; the circulating pipe is connected with the second chamber, so that the whole box body is heated in all directions; the plurality of humidifying pipes are respectively connected with the circulating pipe and the first chamber and used for carrying out multi-point input on water vapor, and the problem of overlarge local humidity caused by single-point input is solved.
Description
Technical Field
The utility model relates to a cell culture technical field especially relates to a temperature and humidity adjustable biological cell culture device.
Background
One cell can be cultured into a simple single cell or a few differentiated multiple cells using cell culture, and thus, cell culture is a fundamental and central technology in biotechnology.
However, when cell culture is performed, most of culture devices are relatively simple and cannot be used for effective temperature and humidity adjustment, so that the temperature and humidity of the culture environment are constantly changed, cell culture is not facilitated, and the efficiency and success rate of cell culture are reduced.
Most conventional culture devices are directly heated, and the problems of local over-fast temperature rise, uneven temperature and the like are easy to occur. When the humidity is adjusted, it is simply connected to a humidifier, or a container of water is placed in the culture apparatus. The humidity adjusting method is simple and rough, and is easy to cause the problems of overhigh local humidity, uneven humidity and the like.
Therefore, a temperature and humidity adjustable culture apparatus is needed to avoid the problems of local over-high temperature and humidity, uneven temperature and humidity, and the like.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a temperature and humidity adjustable biological cell culture device aiming at the defects in the prior art.
In order to achieve the purpose, the utility model adopts the technical proposal that:
the utility model provides a temperature humidity adjustable biological cell culture device, includes:
the box body is sequentially provided with a first chamber, a second chamber and a third chamber from top to bottom, and the front part of the box body is provided with a control panel;
the circulating pipe is in an inverted U shape and is arranged on the inner wall of the box body, and two ends of the circulating pipe are respectively connected with the second chamber;
the humidifying pipe is arranged in the inner wall of the box body, the first end of the humidifying pipe is connected with the circulating pipe, and the second end of the humidifying pipe is communicated with the first chamber;
the heating plate is arranged in the first cavity and is electrically connected with the control panel;
a first end of the connecting pipe is connected with the second chamber, and a second end of the connecting pipe is connected with the box body;
the exhaust device is arranged at the upper part of the box body in a penetrating way;
the humidity detection element is positioned in the first cavity and is arranged on the inner wall of the box body, and the humidity detection element is electrically connected with the control panel;
the temperature detection element is located in the first cavity and mounted on the inner wall of the box body, and the temperature detection element is electrically connected with the control panel.
Preferably, the method further comprises the following steps:
the first one-way filter screen is arranged at the upper end and/or the lower end of the exhaust device.
Preferably, the first one-way filter net positioned at the upper end of the exhaust device is conical or semicircular.
Preferably, the method further comprises the following steps:
and the air inlet device is penetratingly arranged on the side part of the box body, and the second end of the air inlet device is connected with the circulating pipe.
Preferably, the method further comprises the following steps:
the second one-way filter screen is arranged at the first end of the air inlet device.
Preferably, the second end of the exhaust device is positioned outside the box body, and the outer diameter of the second end of the exhaust device is larger than that of the exhaust device;
the second end of the air inlet device is located on the outer side of the box body, and the outer diameter of the second end of the air inlet device is larger than that of the air inlet device.
Preferably, the box body comprises an outer wall, an interlayer and an inner wall, the interlayer is arranged between the outer wall and the inner wall, and at least one of the interlayer and the inner wall is a heat insulation layer.
Preferably, the case includes a viewing window provided at a front portion of the case and corresponding to the second chamber.
Preferably, the circulating pipe is provided with a plurality of circulating pipes which are arranged in parallel.
Preferably, the number of the humidifying pipes is several, one circulating pipe is connected with several humidifying pipes, and the axial directions of several humidifying pipes are mutually perpendicular and/or mutually parallel and/or mutually collinear on the same circulating pipe.
The utility model adopts the above technical scheme, compare with prior art, have following technological effect:
the utility model discloses a biological cell culture device with adjustable temperature and humidity, the inside of the box body is sequentially arranged into a first cavity, a second cavity and a third cavity from top to bottom, the third cavity heats the second cavity, the evaporation effect in the second cavity is utilized to carry out indirect temperature regulation and direct humidity regulation on the first cavity, and the uniformity of the regulation of temperature and humidity is ensured; the circulating pipe is connected with the second chamber, so that the whole box body is heated in all directions; the plurality of humidifying pipes are respectively connected with the circulating pipe and the first chamber and used for carrying out multi-point input on water vapor, and the problem of overlarge local humidity caused by single-point input is solved.
Drawings
Fig. 1 is a schematic diagram of an exemplary embodiment of the present invention.
Fig. 2 is a cross-sectional view of an exemplary embodiment of the present invention.
Fig. 3 is a schematic diagram of another embodiment of the present invention.
Fig. 4 is a cross-sectional view of another embodiment of the present invention.
Wherein the reference numerals are: the device comprises a box body 1, a first chamber 2, a second chamber 3, a third chamber 4, a box door 5, a control panel 6, a display screen 7, an observation window 8, a circulating pipe 9, a humidifying pipe 10, a heating plate 11, a connecting pipe 12, an exhaust device 13, a humidity detection element 14, a temperature detection element 15, a first one-way filter screen 16, an air inlet device 17, a second one-way filter screen 18, an outer wall 19, an interlayer 20, an inner wall 21 and a shelving plate 22.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.
It should be noted that, in the present invention, the embodiments and features of the embodiments may be combined with each other without conflict.
The present invention will be further described with reference to the accompanying drawings and specific embodiments, but the present invention is not limited thereto.
Example 1
The embodiment is an illustrative embodiment of the utility model, as shown in fig. 1-2, a temperature and humidity adjustable biological cell culture device, including box 1, exhaust apparatus 13 and air inlet unit 17, exhaust apparatus 13 sets up on the upper portion of box 1 with running through, and air inlet unit 17 sets up the lateral part at box 1 with running through, and exhaust apparatus 13 and air inlet unit 17 are used for controlling the circulation of gas in the box 1.
The front portion of the box body 1 is rotatably provided with a box door 5, the front portion of the box body 1 is provided with a control panel 6, a display screen 7 and an observation window 8, the control panel 6 and the display screen 7 are located on the side portion of the box door 5, the observation window 8 is located on the lower portion of the box door 5, and the display screen 7 is coupled with the control panel 6.
Further, the middle portion of door 5 is made of a transparent material, including but not limited to glass.
The inside of box 1 is provided with first cavity 2, second cavity 3 and third cavity 4, separates each other between first cavity 2, second cavity 3 and the third cavity 4, each other not communicates. Wherein, the first chamber 2 is a culture chamber, the second chamber 3 is a water chamber, and the third chamber 4 is a heating chamber.
In the first chamber 2, a number of resting plates 22 are provided at intervals for placing cell culture devices, such as culture dishes, culture tubes, etc.
In first cavity 2, a plurality of humidity detecting element 14 and a plurality of temperature detecting element 15 are still installed to the inner wall of box 1, are used for detecting humidity and temperature in first cavity 2 respectively, and humidity detecting element 14 and temperature detecting element 15 are coupled with power and display screen 7 respectively to carry the humidity information and the temperature information that detect the acquisition to display screen 7, supply operating personnel to carry out temperature and humidity control and refer to.
The exhaust device 13 is disposed through an inner wall of the case 1, and is configured to exhaust the gas in the first chamber 2 to the outside of the case 1, thereby improving the fluidity of the gas in the first chamber 2.
Further, a first one-way filter 16 is disposed at a lower end of the exhaust device 13 for preventing external dust from entering the first chamber 2 through the exhaust device 13.
Further, the second end of the exhaust device 13 is located outside the box body 1, and the outer diameter of the second end is larger than the outer diameter of the exhaust device 13, that is, the longitudinal section of the exhaust device is in an inverted trapezoid shape, so that when the gas in the first chamber 2 is exhausted outwards, the flow rate of the gas is improved.
The air inlet device 17 penetrates through the inner wall of the box body 1, is used for inputting fresh air into the first chamber 2, and forms an air passage with the exhaust device 13, so that the air fluidity in the first chamber 2 is improved.
Further, a second one-way filter 18 may be provided at a first end of the air inlet means 17 for preventing external dust from entering the first chamber 2 through the air inlet means 17.
Further, the first end of the air inlet device 17 is located outside the box body 1, and the outer diameter of the first end is larger than the outer diameter of the air inlet device 17, that is, the longitudinal section of the air inlet device is trapezoidal, so that a "narrow pipe effect" is formed when gas is input into the first chamber 2, and the flow rate of the gas entering the first chamber 2 is improved.
Further, the exhaust device 13 and the intake device 17 are diagonally disposed, that is, the exhaust device 13 is located at the left side of the upper portion of the case 1, and the intake device 17 is located at the lower side of the right portion of the case 1.
Further, a sealing plug (not shown) is disposed at the connection position of the connection pipe 12 and the box body 1 for blocking the connection pipe and preventing the water vapor from overflowing from the connection pipe.
Further, the connecting pipe 12 is inclined from bottom to top, the lower end thereof is connected with the second chamber 3, and the upper end thereof is connected with the box body 1.
Wherein, the observation window 8 of box 1 is just right second chamber 3 for observe the liquid level in the second chamber 3 through observation window 8.
At least one circulation pipe 9 is arranged in the inner wall of the box body 1, the circulation pipe 9 is in an inverted U shape, two ends of the circulation pipe 9 are connected with two sides of the second chamber 3, and the circulation pipe 9 is used for enabling water vapor evaporated by the second chamber 3 to flow in the circulation pipe 9, so that the inner walls of the box body 1 on the left side, the right side and the upper side of the first chamber 2 are heated, and the first chamber 2 is heated externally.
Therefore, the upper side, the lower side, the left side, the right side and the inside of the first chamber 2 are heated by the heat sources, and the uniformity of temperature rise is ensured.
Specifically, the water vapor flows in the circulation pipe 9, and when it condenses, it flows back into the second chamber 3 by gravity, i.e., the water vapor flows upward from both sides of the circulation pipe 9, and the condensed water flows downward from both sides of the circulation pipe 9.
The circulation pipe 9 may be plural, and plural circulation pipes 9 are provided in parallel with each other for further improving the uniformity of temperature adjustment.
At least one humidifying pipe 10 is provided in the inner wall of the cabinet 1, and has a first end connected to the circulation pipe 9 and a second end communicating with the first chamber 2, for inputting the water vapor in the circulation pipe 9 into the first chamber 2.
Further, the humidifying pipe 10 may be plural, and each circulation pipe 9 may be connected to plural humidifying pipes 10 for inputting water vapor into the first chamber 2 from different positions.
Wherein, on the same circulating pipe 9, the axial directions of the plurality of humidifying pipes 10 connected with the same circulating pipe can be vertical to each other, parallel to each other and collinear with each other.
Specifically, a first humidifying pipe 10 is disposed perpendicular to the horizontal direction, a second humidifying pipe 10 is disposed parallel to the horizontal direction, and a third humidifying pipe 10 is disposed parallel to the horizontal direction but not in line with the second humidifying pipe 10.
Further, a solenoid valve (not shown) is provided at the second end of each humidifying pipe 10, and the solenoid valve is coupled to the control panel 6 for adjusting the amount of water vapor discharged from the humidifying pipe 10 under the action of the control panel 6.
For the plurality of circulation pipes 9 and the plurality of humidifying pipes 10, the circulation pipes 9 and the humidifying pipes 10 are in a ratio of 1: n, wherein n can be dynamically adjusted.
In particular, the humidifying pipes 10 are provided at intervals for the adjacent circulation pipes 9, i.e. on the first circulation pipe 9, the left side, the upper side and the right side of the water tank are respectively provided with a humidifying pipe 10, and on the second circulating pipe 9 at the adjacent side, the left, upper and right sides thereof are provided with two humidifying pipes 10, respectively, in the order of a first humidifying pipe 10 on the left side of the second circulation pipe 9, a humidifying pipe 10 on the left side of the first circulation pipe 9, a second humidifying pipe 10 on the left side of the second circulation pipe 9, a first humidifying pipe 10 on the upper side of the second circulation pipe 9, a humidifying pipe 10 on the upper side of the first circulation pipe 9, a second humidifying pipe 10 on the upper side of the second circulation pipe 9, a first humidifying pipe 10 on the right side of the second circulation pipe 9, a humidifying pipe 10 on the right side of the first circulation pipe 9, and a second humidifying pipe 10 on the right side of the second circulation pipe 9, as viewed from the front.
Through the arrangement, a three-dimensional water vapor input network can be formed, and the uniformity of temperature and humidity adjustment is ensured.
Further, the air inlet device 17 is connected to the circulating pipe 9, and functions to heat the gas input from the air inlet device 17 by using water vapor, so as to prevent the gas with lower temperature from entering the first chamber 2 and affecting the temperature in the first chamber 2.
Inside the third chamber 4 there is fixedly mounted a heating plate 11, which is coupled to the power supply and control panel 6, respectively, for heating the water inside the second chamber 3.
Further, the box body 1 is composed of an outer wall 19, an interlayer 20 and an inner wall 21, and the outer wall 19, the interlayer 20 and the inner wall 21 form a sandwich structure for reducing temperature loss in the box body 1.
Further, the interlayer 20 is an insulation layer made of an insulation material.
Further, the inner wall 21 may also be a thermal insulation layer made of a thermal insulation material, whose thermal conductivity is smaller than that of the interlayer 20.
Further, the thickness of the inner wall 21 is greater than the thickness of the interlayer 20.
Further, the outer wall 19, the interlayer 20 and the inner wall 21 may be directly connected, such as by bonding, or indirectly connected, such as by providing a vacuum layer.
When the vacuum layers are arranged between the outer wall 19 and the interlayer 20 and between the interlayer 20 and the inner wall 21, the heat insulation capability of the box body 1 can be improved to the greatest extent, and the temperature loss in the box body 1 is reduced.
Further, the circulation pipe 9 is disposed in the interlayer 20, and the humidifying pipe 10 is communicated with the first chamber 2 after passing through the interlayer 20 and the inner wall 21 in sequence.
The method of use of this example is as follows: removing the sealing plug, inputting water into the third chamber 4 through the connecting pipe 12, and after inputting a certain volume of water, using the sealing plug to block the connecting pipe 12; the heating plate 11 is controlled to work by the control panel 6 on the box body 1; at this time, under the action of the heating plate 11, the water in the second chamber 3 starts to evaporate; the working power of the heating plate 11 is adjusted through the humidity information and the temperature information which are transmitted to the display screen 6 by the humidity detection element 14 and the temperature detection element 15, the liquid level in the second chamber 3 is observed according to the observation window 8, and the water is supplemented at all times; the culture apparatus is put into the resting plate 22, and then the door 5 is closed; in order to control the humidity in the cabinet 1, a large amount of water may be added to the second chamber 3, or the water in the second chamber 3 may be pumped out to ensure that the first chamber 2 of the cabinet 1 is in a constant temperature and humidity state.
The embodiment has the advantages that the second chamber is heated through the third chamber, and the first chamber is subjected to indirect temperature regulation and direct humidity regulation by utilizing the evaporation effect in the second chamber, so that the uniformity of temperature and humidity regulation is ensured; the circulating pipe is connected with the second chamber, so that the whole box body is heated in all directions; the plurality of humidifying pipes are respectively connected with the circulating pipe and the first chamber and used for carrying out multi-point input on water vapor, and the problem of overlarge local humidity caused by single-point input is solved.
Example 2
The utility model discloses a another embodiment, as shown in fig. 3 ~ 4, a biological cell culture device of adjustable humiture, including box 1, exhaust apparatus 13 and air inlet unit 17, exhaust apparatus 13 sets up on the upper portion of box 1 with running through, and air inlet unit 17 sets up the lateral part at box 1 with running through, and exhaust apparatus 13 and air inlet unit 17 are used for controlling the circulation of gas in the box 1.
The structure and connection relationship of the box 1, the exhaust device 13 and the intake device 17 are substantially the same as those of embodiment 1, and are not described herein again.
The present embodiment is different from embodiment 1 in the number of exhaust devices 13 and intake devices 17, and the first one-way filter 16 is further provided at the upper end of the exhaust device 13.
The exhaust devices 13 are symmetrically disposed at left and right sides of an upper portion of the case 1. The air intake devices 17 are symmetrically disposed on the left and right sides of the case 1. By this arrangement the gas flow rate in the first chamber 2 is further increased.
As for the first one-way filters 16, they are provided at the upper and lower ends of the exhaust device 13 to perform a double filtering function, and the mesh aperture of the first one-way filter 16 located at the lower end of the exhaust device 13 is smaller than the mesh aperture of the first one-way filter 16 located at the upper end of the exhaust device 13.
Further, the first one-way filter 16 positioned at the upper end of the exhaust device 13 is tapered or semicircular, and when impurities and the like fall on the upper end of the exhaust device 13, the impurities are rapidly dropped from the upper end of the exhaust device 13 through the inclined surface of the first one-way filter 16.
The advantage of this embodiment is that the probability of dust entering the first chamber is further reduced by double filtering of the exhaust; the gas flow rate in the first chamber is further increased by the symmetrically arranged exhaust and intake devices.
The above description is only an example of the preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and those skilled in the art should be able to realize the equivalent alternatives and obvious variations of the present invention.
Claims (10)
1. The utility model provides a temperature humidity adjustable biological cell culture device which characterized in that includes:
the box body is sequentially provided with a first chamber, a second chamber and a third chamber from top to bottom, and the front part of the box body is provided with a control panel;
the circulating pipe is in an inverted U shape and is arranged on the inner wall of the box body, and two ends of the circulating pipe are respectively connected with the second chamber;
the humidifying pipe is arranged in the inner wall of the box body, the first end of the humidifying pipe is connected with the circulating pipe, and the second end of the humidifying pipe is communicated with the first chamber;
the heating plate is arranged in the first cavity and is electrically connected with the control panel;
a first end of the connecting pipe is connected with the second chamber, and a second end of the connecting pipe is connected with the box body;
the exhaust device is arranged at the upper part of the box body in a penetrating way;
the humidity detection element is positioned in the first cavity and is arranged on the inner wall of the box body, and the humidity detection element is electrically connected with the control panel;
the temperature detection element is located in the first cavity and mounted on the inner wall of the box body, and the temperature detection element is electrically connected with the control panel.
2. The temperature and humidity adjustable biological cell culture apparatus according to claim 1, further comprising:
the first one-way filter screen is arranged at the upper end and/or the lower end of the exhaust device.
3. The temperature and humidity adjustable biological cell culture device according to claim 2, wherein the first one-way filter screen located at the upper end of the exhaust device is conical or semicircular.
4. The temperature and humidity adjustable biological cell culture apparatus according to claim 1, further comprising:
and the air inlet device is penetratingly arranged on the side part of the box body, and the second end of the air inlet device is connected with the circulating pipe.
5. The temperature and humidity adjustable biological cell culture apparatus according to claim 4, further comprising:
the second one-way filter screen is arranged at the first end of the air inlet device.
6. The temperature and humidity adjustable biological cell culture device according to claim 4, wherein the second end of the exhaust device is located outside the box body, and the outer diameter of the second end of the exhaust device is larger than that of the exhaust device;
the second end of the air inlet device is located on the outer side of the box body, and the outer diameter of the second end of the air inlet device is larger than that of the air inlet device.
7. The temperature and humidity adjustable biological cell culture device according to claim 1, wherein the box body comprises an outer wall, an interlayer and an inner wall, the interlayer is arranged between the outer wall and the inner wall, and at least one of the interlayer and the inner wall is a heat insulation layer.
8. The temperature and humidity adjustable biological cell culture device according to claim 1, wherein the box body comprises a viewing window, and the viewing window is arranged at the front part of the box body and corresponds to the second chamber.
9. The temperature and humidity adjustable biological cell culture device according to claim 1, wherein the number of the circulation pipes is several, and the circulation pipes are arranged in parallel.
10. The temperature and humidity adjustable biological cell culture device according to claim 9, wherein the number of the humidifying pipes is plural, one of the circulating pipes is connected to the plural humidifying pipes, and the axial directions of the plural humidifying pipes are perpendicular to each other and/or parallel to each other and/or collinear with each other on the same circulating pipe.
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CN202020326596.5U CN211847968U (en) | 2020-03-16 | 2020-03-16 | Temperature and humidity adjustable biological cell culture device |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN113089370A (en) * | 2021-03-25 | 2021-07-09 | 深圳市康达安生物科技有限公司 | Cervical brush encapsulation dialyzing paper humidification device |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN113089370A (en) * | 2021-03-25 | 2021-07-09 | 深圳市康达安生物科技有限公司 | Cervical brush encapsulation dialyzing paper humidification device |
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