CN211847895U - Electrode - Google Patents

Abstract

The utility model discloses an electrode, include: the electrode plate is designed into a U shape and is convenient for people to take, when the electrode plate is used, the electrode plate is connected with the electrotransfer instrument by the electric lead, then the electrotransfer instrument is started, the electrode plate is pressed on the muscle or the body surface of a living animal by hands, the exogenous DNA is introduced into the living animal body by a handheld syringe from an injection hole by the syringe, and the electrode plate with the U-shaped structure is simple in design structure and higher in operability; the device can be conveniently operated by one person, and can also conveniently conduct exogenous DNA introduction to the living animal by using the electrode plate, thereby greatly saving the labor cost in the exogenous DNA introduction work.

Description

Electrode

Technical Field

The utility model relates to a bioengineering technical field, concretely relates to electrode.

Background

In the research of DNA vaccines and gene therapy in the world today, a very important technical difficulty is how to improve the efficiency of gene introduction into living cells of animals. Research and development of instruments capable of safely and efficiently introducing exogenous DNA into tissue cells is the key to solving this problem. The living cell is in pulse electric field by using specific electric field, and the permeability of cell membrane can be obviously enhanced, so that exogenous DNA molecules can enter into cell in large quantity, and the effect of DNA vaccine and gene therapy can be improved. The electrode is an important accessory which determines the convenience of operation, so that an electrode with simple structure and strong operability is lacked.

SUMMERY OF THE UTILITY MODEL

The utility model provides an electrode for realize that the operability is strong, simple structure's electrode carries out the purpose of the leading-in experiment of exogenous DNA.

The utility model provides an electrode, include: the device comprises an electrode plate, an electric lead and an electric converter, wherein one end of the electric lead is connected with the electrode plate, and the other end of the electric lead is connected with the electric converter; the electrode plate is of a U-shaped sheet structure, a connecting sheet is arranged at the U-shaped opening end of the electrode plate, and an injection hole is formed in the connecting sheet; and one end of the electrode plate, which is far away from the U-shaped opening, is used for connecting an electric lead.

Preferably, electrode pins are symmetrically arranged along the central symmetry line of the electrode plate and are arranged on the connecting plate.

Preferably, the injection hole is provided between the two electrode needles.

Preferably, two ends of the electric lead are respectively connected with a first plug terminal; the electrode plate is provided with a first plug terminal, a second plug terminal, a third plug terminal, a first plug terminal and a second plug terminal, wherein the first plug terminal is connected with the second plug terminal, and the first plug terminal is connected with the third plug terminal.

Preferably, the method further comprises the following steps: get the device, it includes dead lever, slide bar and chuck to get the device, the dead lever is circular shape member, the chuck includes: a first clip piece and a second clip piece; the fixed rod is connected with the first clamping piece, and a sliding groove is formed in the fixed rod; the opening end of the sliding groove faces the first clamping piece; the left end of the sliding rod is arranged in the sliding groove in a sliding mode, and the right end of the sliding rod extends out of the sliding groove and is connected with a second clamping piece; a crack is arranged between the second clamping piece and the first clamping piece and used for clamping the electrode plate; a spring is further arranged at one end of the sliding rod, which is far away from the second clamping piece, and the other end of the spring is fixed at the bottom of the sliding groove; the outer circumferential wall of the sliding rod is also provided with a deflector rod, a penetrating movable groove is arranged between the outer circumferential wall of the fixed rod and the inner wall of the sliding groove, and the deflector rod extends to the outer circumferential wall of the fixed rod through the movable groove and is used for reciprocating along the axial central line direction of the sliding groove; the first clamping piece is of an L-shaped structure, one end of the L-shaped structure is fixed with the fixed rod, a wire walking hole is formed between the fixed rod and the first clamping piece, and the wire walking hole is used for the wire to penetrate through the gap between the first clamping piece and the second clamping piece.

Preferably, one end of the fixing rod, which is far away from the chuck, is provided with an anti-slip sleeve.

Preferably, the shaft of the fixation rod has a plurality of straps for binding the electrical conductors to the circumferential outer wall of the fixation rod.

Preferably, gaskets are arranged on one surfaces, close to the crack, of the first clamping piece and the second clamping piece.

The utility model has the advantages as follows:

the electrode plate is designed into a U shape, which is convenient for people to take, when in use, the electrode plate is firstly connected with an electric rotating instrument by an electric lead, then the electric rotating instrument is started, the electrode plate is pressed on the muscle or the body surface of a living animal by hands, the injector is held by one hand, exogenous DNA is introduced into the living animal body by the injector from the injection hole, the electrode plate with the U-shaped structure has simple design structure and stronger operability; the device can be conveniently operated by one person, and can also conveniently conduct exogenous DNA introduction to the living animal by using the electrode plate, thereby greatly saving the labor cost in the exogenous DNA introduction work.

Other features and advantages of the present invention will be set forth in the description that follows; and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the apparatus particularly pointed out in the written description and drawings thereof.

The technical solution of the present invention is further described in detail by the accompanying drawings and examples.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention.

In the drawings:

fig. 1 is a schematic view of an electrode plate structure provided by the present invention;

fig. 2 is a schematic view of a connection structure of the clamping device and the electrode plate provided by the present invention;

fig. 3 is a schematic cross-sectional view of the clamping device according to the present invention;

fig. 4 is a schematic view of the chuck structure provided by the present invention;

fig. 5 is a schematic diagram of a cushion block structure provided by the present invention;

reference numerals:

the electrode plate comprises 1-electrode plates, 2-electrode pins, 3-injection holes, 4-second plug terminals, 5-connecting pieces, 6-first plug terminals, 7-electric leads, 8-third plug terminals, 9-electric rotating instruments, 10-fixing rods, 11-anti-skidding sleeves, 12-sliding rods, 13-second clamping pieces, 14-first clamping pieces, 15-wire running holes, 16-movable grooves, 17-deflector rods, 18-sliding grooves, 19-springs, 20-clamping heads, 21-clamping seams and 22-cushion blocks.

Detailed Description

The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are presented herein only to illustrate and explain the present invention, and not to limit the present invention.

According to fig. 1-4, an embodiment of the present invention provides an electrode, comprising: the electrode plate 1, the electric lead 7 and the electric converter 9, wherein one end of the electric lead 7 is connected with the electrode plate 1, and the other end is connected with the electric converter 9; the electrode plate 1 is of a U-shaped sheet structure, a connecting sheet 5 is arranged at the U-shaped opening end of the electrode plate 1, and an injection hole 3 is formed in the connecting sheet 5; and one end of the electrode plate 1, which is far away from the U-shaped opening, is used for connecting an electric lead 7.

The working principle and the beneficial effects of the technical scheme are as follows: the electrode plate 1 is designed into a U shape, which is convenient for people to take, when in use, the electrode plate 1 is firstly connected with an electrotransformation instrument 9 by an electric lead 7, then the electrotransformation instrument 9 is started, the electrode plate 1 is pressed on the muscle or the body surface of a living animal by hands, a hand-held syringe is used for introducing exogenous DNA into the living animal body from an injection hole 3 by the syringe, and the electrode plate 1 with the U-shaped structure has simple design structure and stronger operability; the device can be operated by one person conveniently, and can also conveniently conduct exogenous DNA introduction to the living animal by using the electrode plate 1, thereby greatly saving the labor cost in the exogenous DNA introduction work.

In one embodiment, electrode pins 2 are symmetrically arranged along the central symmetry line of the electrode plate 1, and the electrode pins 2 are arranged on the connecting piece 5.

The working principle and the beneficial effects of the technical scheme are as follows: the electrode needles arranged in the central symmetrical structure can generate electric pulses through the conductivity meter 9, and when exogenous DNA is introduced into living animals, the exogenous DNA can be effectively activated, so that the survival rate and the introduction efficiency of the exogenous DNA are improved.

In one embodiment, the injection hole 3 is provided between two electrode needles 2.

The working principle and the beneficial effects of the technical scheme are as follows: the electric field generated by utilizing the principle of electromagnetism generation acts on the syringe needle head between the electrode needles, when exogenous DNA enters a living animal, electric pulse is generated between the two electrode needles which are detained in the body, the permeability of the cell membrane of the living animal can be effectively activated, and the survival rate and the introduction efficiency of the exogenous DNA are improved.

In one embodiment, the two ends of the electric leads 7 are respectively connected with the first plug terminals 6; the electrode plate 1 is provided with the second and inserts terminal 4 far away from the one end of U type opening, be provided with the third on the electricity converter 9 and insert terminal 8, the first of them end of electric lead 7 connects second and inserts terminal 4, the first of the other end of electric lead 7 connects third and inserts terminal 8 and insert terminal 6.

The working principle and the beneficial effects of the technical scheme are as follows: before use, the first plug terminal 6 at one end of the electric lead 7 is connected with the third plug terminal 8 of the electric converter 9, the first plug terminal 6 at the other end of the electric lead is connected with the second plug terminal 4 of the electrode plate 1, and the first plug terminals 6 at the two ends of the electric lead 7 are in a detachable structure design, so that the electric lead 7 with corresponding length can be selected according to the distance of a living animal for introducing exogenous DNA, and the situation that experiments cannot be carried out due to the fact that the electric lead 7 is not specific can be reduced; if the electric lead 7 has poor contact, a new electric lead 7 can be replaced at any time, so that the experimental operability is improved.

In one embodiment, further comprising: the clamping device comprises a fixed rod 10, a sliding rod 12 and a chuck 20, wherein the fixed rod 10 is a round rod, and the chuck 20 comprises: a first clip piece and a second clip piece; the fixing rod 10 is connected with a first clamping piece 14, and a sliding groove 18 is formed in the fixing rod 10; the open end of the slide slot 18 faces the first jaw 14; the left end of the sliding rod 12 is slidably arranged in the sliding groove 18, and the right end of the sliding rod 12 extends out of the sliding groove 18 and is connected with the second clamping piece 13; a gap 21 is arranged between the second clamping piece 13 and the first clamping piece 14, and the gap 21 is used for clamping the electrode plate 1; a spring 19 is further arranged at one end of the sliding rod 12, which is far away from the second clamping piece 13, and the other end of the spring 19 is fixed at the bottom of the sliding groove 18; the circumferential outer wall of the sliding rod 12 is further provided with a deflector rod 17, a penetrating movable groove 16 is arranged between the circumferential outer wall of the fixed rod 10 and the inner wall of the sliding groove 18, and the deflector rod 17 extends to the circumferential outer wall of the fixed rod 10 through the movable groove 16 and is used for reciprocating motion of the deflector rod 17 along the axial central line direction of the sliding groove 18; the first clamping piece 14 is of an L-shaped structure, one end of the L-shaped structure is fixed to the fixing rod 10, a wire passing hole 15 is formed between the fixing rod 10 and the first clamping piece 14, and the wire passing hole 15 is used for enabling the electric lead 7 to penetrate through a gap 21 between the first clamping piece 14 and the second clamping piece 13.

The working principle of the technical scheme is as follows: the deflector rod, the sliding rod and the second clamping piece are integrated, so that the spring 19 is in a stretching state in an initial state; the elastic force of the spring 19 springs the slide bar open and enables the chuck 20 to be used for clamping the electrode sheet 1; under the condition that the electrode plate 1 is not placed, the first clamping piece 14 is contacted with the second clamping piece 13, one end of the sliding rod 12 is connected with the spring 19, and the spring 19 is compressed in the sliding groove 18 of the fixed rod 10 when being stressed;

according to the fig. 2 and 3, in operation, one hand holds one end of the fixed rod 10, the other hand carries the shift lever 17 leftwards, the shift lever 17 is pushed leftwards by hand to move in the movable groove 16, and simultaneously drives the sliding rod 12 to move leftwards in the sliding groove 18, when the sliding rod 12 moves leftwards, the gap 21 between the second clamping piece 13 of the sliding rod 12 and the first clamping piece 14 at one end of the fixed rod 10 gradually increases; at this time, the electrode plate 1 is put in from the slit 21, and when the electrode plate 1 is put in, one end of the second plug-in terminal 4 of the electrode plate 1 is put in towards the wiring hole of the first clamping piece 14; then, the driving lever is released, the spring 19 springs to the right, the sliding rod is pushed out to the right in the sliding groove, the gap between the second clamping piece and the first clamping piece gradually becomes smaller, and the electrode plate is clamped.

Before clamping the electrode plate 1 by the first clamping piece 14 and the second clamping piece 13, firstly, inserting the first plug terminal 6 at one end of the electric lead 7 into the wire running hole 15, and then inserting the first plug terminal 6 on the electric lead 7 into the second plug terminal 4 of the electrode plate 1, so that the electric converter 9 can conduct the electrode plate 1 through the electric lead 7; finally, the electrode plate 1 is clamped by the first clamping piece 14 and the second clamping piece 13, and the fixing rod 10 is further held by a hand to place the electrode plate 1 at a required position for use;

the first clamping piece 14 and the second clamping piece 13 are used for clamping the U-shaped sheet end of the electrode plate 1, a connecting piece 5 arranged on the electrode plate 1 does not need to be clamped, and the connecting piece 1 is used for being attached to a test body to conduct electrode leading-in work.

The beneficial effects of the above technical scheme are that: the clamping device can effectively avoid that the introduced living cells are large animals or sensitive and dangerous living animal parts, such as the neck of the living animal or the claw of the carnivorous animal and the like, and can be operated by a fixed rod, thereby not only ensuring the efficiency of introducing the exogenous DNA, but also avoiding the condition of being injured by the living animal and effectively saving the labor cost which can be completed by multi-person assistance during injection; the problem of electrode slice 1 that current equipment was equipped with design complicacy and operability is relatively poor is solved.

In one embodiment, the end of the fixing rod 10 remote from the chuck 20 is provided with an anti-slip sleeve 11.

The working principle and the beneficial effects of the technical scheme are as follows: when the electrode is used, one end of the fixed rod is held by hands, the friction force between the hands and the rod piece can be increased by the anti-skidding sleeve, the rod piece is not easy to slide, hand feeling and adhesive force are increased, and operability of the electrode is indirectly improved.

In one embodiment, the shaft of the fixation rod 10 has a plurality of straps for binding the electrical leads 7 to the circumferential outer wall of the fixation rod 10.

The working principle and the beneficial effects of the technical scheme are as follows: during the use, wear the first spigot terminal 6 of electric lead 7 to in the crack 21 of chuck 20 for the first spigot terminal of electric lead 7 is connected with the second spigot terminal 4 of electrode slice 1, then binds electric lead 7 in proper order on dead lever 10 circumference outer wall with the bandage, guarantees at the in-process that uses electrode slice 1, reduces to pull because of electric lead 7 and causes the condition that appears the pine between electrode slice 1 and the chuck 20 and take off, further reduces the staff and is by the winding condition of electric lead 7, can indirectly improve the operating efficiency of electrode slice 7.

In one embodiment, the first jaw 14 and the second jaw 13 are each provided with a pad on a side thereof adjacent to the slit 21.

The working principle and the beneficial effects of the technical scheme are as follows: the gasket is preferably made of a silica gel material; the gasket can play a role in buffering and friction and has elasticity; the gasket can increase the friction force between the first clamping piece 14, the second clamping piece 13 and the electrode plate 1, so that the position of the electrode plate 1 is stable, and the elasticity of the gasket can ensure that the electrode plate 1 is not damaged by a clamp.

As shown in fig. 4 and 5, a cushion block 22 is further arranged on one surface of the connecting piece 5 of the electrode plate 1, and the cushion block 22 and the right side of the first clamping piece 14 are arranged on the same plane, so that the first clamping piece 14 and the connecting piece 5 can be in contact with the surface of a test body at the same time; the first clamping piece 14, the second clamping piece 14 and the cushion block 22 are made of metal materials. The cushion blocks 22 are symmetrically arranged on the connecting sheet 5, and the cushion blocks 22 are positioned between the injection hole 3 and the electrode needle 2. According to the figure 4, the length of the electrode needle 2 is larger than the height dimension of the cushion block 22, namely, one end of the electrode needle 2 far away from the connecting sheet 5 is protruded out of the right side surface of the cushion block 22.

The cushion block 22 can enable the electric energy of the electrode slice 1 to contact with the living animal when the electrode slice 1 conducts the exogenous DNA introduction to the living animal, and the exogenous DNA introduction efficiency is improved.

It will be apparent to those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims and their equivalents, the present invention is also intended to include such modifications and variations.

Claims (8)

1. An electrode, comprising: the electrode plate (1), the electric lead (7) and the electric converter (9), wherein one end of the electric lead (7) is connected with the electrode plate (1), and the other end of the electric lead is connected with the electric converter (9);

the electrode plate (1) is of a U-shaped sheet structure, a connecting sheet (5) is arranged at the U-shaped opening end of the electrode plate (1), and an injection hole (3) is formed in the connecting sheet (5); and one end of the electrode plate (1) far away from the U-shaped opening is used for connecting an electric lead (7).

2. An electrode according to claim 1, characterized in that the electrode pins (2) are symmetrically arranged along the central symmetry line of the electrode sheet (1), and the electrode pins (2) are arranged on the connecting sheet (5).

3. An electrode according to claim 2, characterized in that the injection hole (3) is provided between two electrode needles (2).

4. An electrode according to claim 1, characterized in that the electrical conductor (7) is connected at both ends to the first connector terminal (6); the electrode slice (1) is provided with a second plug terminal (4) far away from one end of the U-shaped opening, a third plug terminal (8) is arranged on the electric converter (9), the first plug terminal (6) at one end of the electric lead (7) is connected with the second plug terminal (4), and the first plug terminal (6) at the other end of the electric lead (7) is connected with the third plug terminal (8).

5. An electrode according to claim 1, further comprising: the clamping device comprises a fixed rod (10), a sliding rod (12) and a chuck (20), the fixed rod (10) is a round rod piece,

the chuck (20) comprises: a first jaw (14) and a second jaw (13); the fixing rod (10) is connected with a first clamping piece (14), and a sliding groove (18) is formed in the fixing rod (10); the open end of the sliding groove (18) faces the first clamping piece (14);

the left end of the sliding rod (12) is arranged in the sliding groove (18) in a sliding mode, and the right end of the sliding rod (12) extends out of the sliding groove (18) and is connected with the second clamping piece (13);

a crack (21) is arranged between the second clamping piece (13) and the first clamping piece (14), and the crack (21) is used for clamping the electrode plate (1); a spring (19) is further arranged at one end, away from the second clamping piece (13), of the sliding rod (12), and the other end of the spring (19) is fixed to the bottom of the sliding groove (18);

a shifting lever (17) is further arranged on the circumferential outer wall of the sliding rod (12), a penetrating movable groove (16) is formed between the circumferential outer wall of the fixed rod (10) and the inner wall of the sliding groove (18), the shifting lever (17) extends to the circumferential outer wall of the fixed rod (10) through the movable groove (16), and the shifting lever (17) reciprocates along the axial central line direction of the sliding groove (18);

the wire fixing device is characterized in that the first clamping piece (14) is of an L-shaped structure, one end of the L-shaped structure is fixed to the fixing rod (10), a wire passing hole (15) is formed between the fixing rod (10) and the first clamping piece (14), and the wire passing hole (15) is used for a wire (7) to penetrate through a gap (21) between the first clamping piece (14) and the second clamping piece (13).

6. An electrode according to claim 5, characterized in that the end of the fixing rod (10) remote from the clamping head (20) is provided with an anti-slip sleeve (11).

7. An electrode according to claim 5, characterised in that the shaft of the fixation rod (10) has a plurality of straps for binding the electrical lead (7) to the circumferential outer wall of the fixation rod (10).

8. An electrode according to claim 5, wherein the first (14) and second (13) jaws are provided with a gasket on their faces adjacent the gap (21).

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