CN211847885U

CN211847885U - RNA draws kit fast

Info

Publication number: CN211847885U
Application number: CN202020039065.8U
Authority: CN
Inventors: 谢严
Original assignee: Shanghai Yishan Biotechnology Co ltd
Current assignee: Shanghai Yishan Biotechnology Co ltd
Priority date: 2020-01-09
Filing date: 2020-01-09
Publication date: 2020-11-03
Anticipated expiration: 2030-01-09

Abstract

The utility model discloses a kit is drawed fast to RNA, including drawing the case, the joint of top outside department of drawing the case has lower plate body, and the top outer wall of lower plate body is the matrix and distributes and arrange the hole with the ware body, the plate body is fixed with the plasmid extractor through the hole of arranging of the ware body down, and the top outer wall joint of plasmid extractor has the upper plate body, the top outer wall of upper plate body is the matrix and distributes and has ware body fixed orifices, and the top outer wall joint of plasmid extractor is on the inner wall of ware body fixed orifices. The utility model discloses an upper and lower plate body is the individuality that the plasmid extractor of checkered structure will be experimented divide into the different position of moving about freely and quickly, during the record of every check extract product in the extraction drawer, is only location, reduces and obscures and the deviation, reduces the accidental error in the experimental data record, utilizes the plasmid extractor to replace traditional test tube or kit, adopts classic silica gel membrane adsorption technology, can be specific, adsorb RNA high-efficiently to obtain the high-purity plasmid.

Description

RNA draws kit fast

Technical Field

The utility model relates to an extract kit technical field, especially relate to a RNA draws kit fast.

Background

Ribonucleic acid (abbreviated as RNA, RibonucleicAcid), a genetic information carrier present in biological cells and in parts of viruses, viroids. RNA is a long chain molecule formed by the condensation of ribonucleotides via phosphodiester bonds. One ribonucleotide molecule consists of a phosphate, a ribose and a base. RNA has 4 kinds of bases, namely A adenine, G guanine, C cytosine, U uracil, wherein U (uracil) replaces T in DNA.

The existing RNA is extracted quickly through a kit, but the extraction effect is not high in purity, and meanwhile, a series of substances extracted cannot be well contrasted and distinguished in the extraction process, so that the data record in the experimental result is influenced.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing a rapid RNA extraction kit.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a rapid RNA extraction kit comprises an extraction box, a lower plate body is clamped at the outer edge of the top of the extraction box, and the outer wall of the top of the lower plate body is distributed with device body arrangement holes in a matrix shape, the lower plate body is fixed with a plasmid extractor through the device body arrangement holes, and the top outer wall of the plasmid extractor is clamped with an upper plate body, the top outer wall of the upper plate body is distributed with device body fixing holes in a matrix form, the top outer wall of the plasmid extractor is clamped on the inner wall of the device body fixing hole, the bottom inner wall of the extraction box is clamped with a slot, and an extraction drawer is inserted in the inner wall of the slot, the extraction drawer comprises a drawing body, two inner baffles, a first target body and three second target bodies, the two inner baffles are distributed on the inner wall of the drawing body in parallel at equal intervals, the first mark body is positioned in the middle of the outer wall of the end part of the drawing body, and the three second mark bodies are distributed on the outer wall of one side of the drawing body, which is close to the outer wall divided by the two inner partition plates.

As the utility model discloses further scheme, two fixed slots of the top outer wall symmetric distribution of upper plate body, and two equal joints of fixed slot inner wall have the contrast baffle, the upper plate body divide into the trisection structure through two contrast baffles.

As a further proposal of the utility model, the plasmid extractor comprises a collecting pipe, an adsorption column, a sieve plate and an attached membrane pressing ring, and the adsorption column is clamped on the inner wall of the bottom end of the collecting pipe.

As a further proposal of the utility model, the membrane pressing ring is integrally arranged on the outer wall of the bottom end of the collecting pipe, and the sieve plate is connected with the joint of the membrane pressing ring and the collecting pipe.

As a further aspect of the present invention, the attached membrane pressing ring includes a pressing ring and a silicone membrane, and the silicone membrane is connected to the inner wall of the pressing ring.

As a further scheme of the utility model, it has the draw-in groove to draw the top outside of case to open, and draw-in groove inner wall joint has the sealing washer, the plate body passes through the sealing washer down and draws case looks joint.

The utility model has the advantages that:

1. the RNA rapid extraction kit is divided into a three-layer structure, an experimental plasmid extractor is divided into individuals at different longitudinal and transverse positions by the upper plate body and the lower plate body in a grid-shaped structure, and the plasmids are uniquely positioned when recording of each grid of extracts in an extraction drawer in the bottom, so that confusion and deviation are reduced, and accidental errors in experimental data recording are reduced;

2. the RNA rapid extraction kit utilizes the plasmid extractor to replace a traditional test tube or a traditional kit, the plasmid extractor consists of five parts, namely a collecting tube, an adsorption column, a sieve plate, a silica gel membrane and a clamping ring, and the RNA can be adsorbed specifically and efficiently by adopting the classical silica gel membrane adsorption technology, so that high-purity plasmids are obtained.

Drawings

FIG. 1 is a schematic diagram of the general structure of a rapid RNA extraction kit according to the present invention;

FIG. 2 is a schematic diagram of a plasmid extractor of the rapid RNA extraction kit according to the present invention;

fig. 3 is a schematic diagram of the structure of the extraction drawer of the RNA rapid extraction kit according to the present invention.

In the figure: 1 extraction box, 2 extraction drawer, 3 lower plate body, 4 plasmid extractor, 5 device body arrangement holes, 6 device body fixing holes, 7 contrast baffle, 8 upper plate body, 9 collecting pipe, 10 adsorption column, 11 sieve plate, 12 membrane attaching pressing ring, 13 first mark body, 14 second mark body and 15 inner baffle.

Detailed Description

The technical solution of the present patent will be described in further detail with reference to the following embodiments.

Reference will now be made in detail to embodiments of the present patent, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present patent and are not to be construed as limiting the present patent.

In the description of this patent, it is to be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the patent and for the simplicity of description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the patent.

In the description of this patent, it is noted that unless otherwise specifically stated or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly and can include, for example, fixedly connected, disposed, detachably connected, disposed, or integrally connected and disposed. The specific meaning of the above terms in this patent may be understood by those of ordinary skill in the art as appropriate.

Referring to fig. 1-3, a kit for rapid extraction of RNA comprises an extraction box 1, a lower plate 3 is clamped at the outer edge of the top of the extraction box 1, device body arrangement holes 5 are distributed on the outer wall of the top of the lower plate 3 in a matrix form, a plasmid extractor 4 is fixed on the lower plate 3 through the device body arrangement holes 5, an upper plate 8 is clamped on the outer wall of the top of the plasmid extractor 4, device body fixing holes 6 are distributed on the outer wall of the top of the upper plate 8 in a matrix form, the outer wall of the top of the plasmid extractor 4 is clamped on the inner wall of the device body fixing holes 6, a slot is clamped on the inner wall of the bottom of the extraction box 1, an extraction drawer 2 is inserted into the inner wall of the slot, the extraction drawer 2 comprises a drawer, two inner baffles 15, a first target 13 and three second target 14, the two inner baffles 15 are equidistantly and parallelly distributed on the inner wall of the drawer, the first target 13 is located in the middle of the, and three second mark body 14 distributes on the outer wall that one side outer wall of the pull body is close to two inner baffles 15 and cuts apart, two fixed slots of top outer wall symmetric distribution of upper plate body 8, and two fixed slot inner walls all joint have contrast baffle 7, upper plate body 8 divide into the trisection structure through two contrast baffles 7, plasmid extractor 4 includes collecting pipe 9, adsorption column 10, sieve 11 and attaches membrane clamping ring 12, and adsorption column 10 joint is on the bottom inner wall of collecting pipe 9, attach membrane clamping ring 12 an organic whole and establish on the bottom outer wall of collecting pipe 9, and sieve 11 joint is in the junction that attaches membrane clamping ring 12 and collecting pipe 9, attach membrane clamping ring 12 includes clamping ring and pellosil, and the pellosil joint is on the inner wall of clamping ring, the outside of the top of extraction case 1 is opened there is the draw-in groove, and draw-in groove inner wall joint has the sealing washer, lower plate body 3 through sealing washer and extraction case 1 looks joint.

The working principle is as follows: when the RNA rapid extraction kit is used, extracts to be extracted are injected into a plasmid extractor 4, after standing, the extracts are fixed on a lower plate body 3 and then pressed into an upper plate body 8, the plasmid extractor 4 is divided into I, II and III groups which are longitudinally arranged through two comparison partition plates 7, the plasmids are extracted through the plasmid extractor 4, the plasmid extractor 4 consists of a collecting pipe 9, an adsorption column 10, a sieve plate 11 and an attached membrane pressing ring 12, the RNA can be specifically and efficiently adsorbed by adopting the classical silica gel membrane adsorption technology, so that high-purity plasmids are obtained and collected into an extraction drawer 2, the extraction drawer is divided into 1-N transverse groups, the corresponding position of the extract of each group is determined by the longitudinal position and the transverse position during positioning, for example, the I-3 is the extract of the plasmid extractor 4 in the third row hole of the I line.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The utility model provides a kit for rapidly extracting RNA, includes and draws case (1), its characterized in that, draw the top outside department joint of case (1) to have lower plate body (3), and the top outer wall of lower plate body (3) is the matrix and distributes and arrange hole (5) with the ware body, lower plate body (3) is fixed with plasmid extractor (4) through arranging hole (5) with the ware body, and the top outer wall joint of plasmid extractor (4) has upper plate body (8), the top outer wall of upper plate body (8) is the matrix and distributes ware body fixed orifices (6) with the ware body fixed orifices, and the top outer wall joint of plasmid extractor (4) is on the inner wall of ware body fixed orifices (6), the bottom inner wall joint that draws case (1) has the slot, and the slot inner wall is pegged graft and is had and draws drawer (2), it includes the drawing body to draw drawer (2), two inner baffles (15), first mark body (13) and three second mark body (14), the two inner baffles (15) are distributed on the inner wall of the drawing body in parallel at equal intervals, the first mark body (13) is positioned in the middle of the outer wall of the end part of the drawing body, and the three second mark bodies (14) are distributed on the outer wall of one side of the drawing body, which is close to the outer wall divided by the two inner baffles (15).

2. The rapid RNA extraction kit of claim 1, wherein the top outer wall of the upper plate body (8) is symmetrically provided with two fixing grooves, the inner walls of the two fixing grooves are clamped with contrast partition plates (7), and the upper plate body (8) is divided into three equal parts by the two contrast partition plates (7).

3. The kit for rapidly extracting RNA as defined in claim 1, wherein the plasmid extractor (4) comprises a collection tube (9), an adsorption column (10), a sieve plate (11) and a membrane-attached pressing ring (12), and the adsorption column (10) is clamped on the inner wall of the bottom end of the collection tube (9).

4. The kit for rapidly extracting RNA as defined in claim 3, wherein the membrane-attached pressing ring (12) is integrally arranged on the outer wall of the bottom end of the collection tube (9), and the sieve plate (11) is clamped at the joint of the membrane-attached pressing ring (12) and the collection tube (9).

5. The kit for rapidly extracting RNA as defined in claim 4, wherein the membrane-attached pressing ring (12) comprises a pressing ring and a silicone membrane, and the silicone membrane is clamped on the inner wall of the pressing ring.

6. The kit for rapidly extracting RNA as defined in claim 1, wherein a clamping groove is formed at the outer edge of the top of the extraction box (1), a sealing ring is clamped on the inner wall of the clamping groove, and the lower plate body (3) is clamped with the extraction box (1) through the sealing ring.